KR102516049B1 - Vulvar cleaning composition that targets vaginitis bacteria - Google Patents

Abstract

The present invention relates to a vulvar cleaning composition for targeting antibacterial vaginitis.
According to one embodiment, in the vulvar cleaner composition for selectively targeting antibacterial vaginitis causing vaginitis, the vulvar cleaner composition containing octenidine, a naturally-derived phenolic compound and a foaming agent as active ingredients can be provided. .

Description

Vulva cleaning composition targeting vaginitis bacteria {VULVAR CLEANING COMPOSITION THAT TARGETS VAGINITIS BACTERIA}

The present invention relates to a vulvar cleaning composition for targeting antibacterial vaginitis.

Lactobacillus, which maintains weak acidity, is present in the female genitourinary system to prevent the growth of harmful bacteria. However, in addition to physical damage and hormonal imbalance, various diseases are easily caused by unclean environment and bacterial or sexually transmitted diseases. .

In order to prevent these various diseases, various vulvar cleaning compositions are used, and most of these vulvar cleaning compositions are natural extracts, natural materials containing lactic acid bacteria, and bactericidal ingredients including 1,2 hexanediol, ethylhexylglycerin, and sodium benzoate It is made by mixing the materials of

However, existing vulvar cleaning compositions have weak bactericidal power and emphasize stability, so it is difficult to see practical effects.

On the other hand, in order to increase the sterilizing power of the existing vulva cleaning composition, some studies are being conducted to apply octenidine hydrochloride used in products in the skin cleaning field, but when mixing octenidine components, there are limitations in the manufacturing process. There was a problem in that solvents with high skin irritation, such as ethanol and phenoxyethanol, had to be used together.

Accordingly, the development of a safe and fast sterilizing vulvar cleaner composition without using irritating substances is attracting attention, and more specifically, it does not kill beneficial bacteria such as vaginal lactic acid bacteria that produce lactic acid in the vagina and causes vaginitis. It is necessary to develop a detergent composition capable of selectively killing only harmful bacteria.

The vulvar cleaner composition for target antibacterial vaginitis according to an embodiment of the present invention is proposed to solve the above problems, and even if it contains octenidine, it has low skin irritation and also eliminates odor and inflammation through cleanliness. To prevent, and to provide a vulvar cleaning composition having a selective antibacterial activity.

According to one embodiment, in the vulvar cleaner composition for selectively targeting antibacterial vaginitis causing vaginitis, the vulva cleaner composition containing octenidine, a naturally-derived phenolic compound and a foaming agent as active ingredients can be provided. .

In addition, the octenidine is octenidine hydrochloride (Octenidine dihydrochloride), the vulva cleaning composition may be provided.

In addition, the vulva cleaner composition containing one or more substances selected from the group consisting of thymol, menthol, and isopropylmethylphenol as the naturally-derived phenolic compound may be provided.

In addition, the foam forming agent is any one material or one or more materials selected from cocamidopropyl betaine, coco betaine, sodium lauroyl oat amino acid, lauryl sulfate, polysorbate and lauramine oxide. A vulvar cleanser composition comprising 　 can be provided.

In addition, the vulva cleaning composition may be provided with a 　 vulva cleaning composition further containing a bubble retaining agent and a deodorant as auxiliary components.

In addition, the foam retaining agent may be provided with glycerin in a vulva cleaning composition.

In addition, the bubble-retaining agent is contained in the vulva cleaning composition only when the vulvar cleaning composition contains the cocamidopropyl betaine as an active ingredient, the vulvar cleaning composition may be provided.

In addition, the deodorant may be provided with a vulva cleaning composition containing any one material selected from sugar cane extract, cypress extract, lemon extract, eucalyptus extract, and zeolite.

In addition, the vulva cleaning composition includes 0.01 to 5% by weight of octenidine, 0.01 to 1% by weight of natural phenolic compounds, 1 to 10% by weight of foam forming agent, 0 to 1.5% by weight of foam retaining agent, and 0.05 to 0.15% by weight of deodorant. % and 90 to 97% by weight of purified water may be provided.

The vulvar cleaner composition for target antibacterial vaginitis according to an embodiment of the present invention has low skin irritation even if it contains octenidine, prevents odor and inflammation through cleanliness, and has selective antibacterial activity.

1 is a view showing octenidine and isopropylmethylphenol, which are main components of a vulva cleaner composition for targeting antibacterial vaginitis according to embodiments of the present invention.
Figure 2 is a view comparing the deodorizing power of Examples 1 and 2 according to the present invention and Comparative Example 1.
3 is a view showing the results of testing the bactericidal power on Gram-positive bacteria and Gram-negative bacteria of Example 1 in the present invention.
Figure 4 is a view for explaining the principle of selective antibacterial activity of the vulva cleaning composition for targeting antibacterial vaginitis according to embodiments of the present invention.
5 is a view showing the results of testing the bactericidal power against the herpes virus of Example 1 in the present invention.

Other advantages and features of the present invention, and methods for achieving them, will become clear with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below and may be implemented in various different forms, but only the present embodiments make the disclosure of the present invention complete, and the common knowledge in the art to which the present invention belongs It is provided to fully inform the holder of the scope of the invention, and the present invention is only defined by the scope of the claims.

Even if not defined, all terms (including technical or scientific terms) used herein have the same meaning as generally accepted by common technology in the prior art to which this invention belongs. Terms defined by general dictionaries may be interpreted to have the same meaning as they have in the related art and/or the text of the present application, and are not conceptualized or overly formalized, even if not expressly defined herein. won't

Terms used in this specification are for describing embodiments and are not intended to limit the present invention. In this specification, singular forms also include plural forms unless specifically stated otherwise in a phrase. As used in the specification, 'comprise' and/or various conjugations of this verb, such as 'comprise', 'comprising', 'comprising', 'comprising', etc., refer to a mentioned composition, ingredient, component, Steps, acts and/or elements do not preclude the presence or addition of one or more other compositions, ingredients, components, steps, acts and/or elements. In this specification, the term 'and/or' refers to each of the listed elements or various combinations thereof.

Hereinafter, specific embodiments of the present invention will be described in detail with reference to the drawings.

In addition, in describing the present invention, if it is determined that a detailed description of a related known configuration or function may obscure the gist of the present invention, the detailed description will be omitted.

In the present invention, octenidine is prepared to include at least one naturally occurring phenolic compound and at least one foam forming agent, but further include a foam retaining agent and a deodorant, thereby maximizing the bactericidal power, which is an advantage of octenidine, A vulvar cleaner composition designed to alleviate skin irritation, which is a disadvantage of octenidine, prevent odor and inflammation, and selectively antibacterial vaginitis was secured.

In addition, the vulvar cleaning composition of the present invention can be used as a human body cleaning agent classified as cosmetics, and is specifically a cleaning agent used for the purpose of cleaning the vulva.

In addition, the vulva cleaning composition of the present invention can be used as a vulvar disinfectant classified as a quasi-drug, and is specifically a disinfectant used for sterilization and disinfection of the skin to prevent infection.

That is, the present invention can be used as a feminine cleanser product for the purpose of cleaning and beautifying the vulva and having a slight action on the human body, and furthermore, the present invention is aimed at treating and preventing inflammation of the vulva and has a stronger action than cosmetics It can be used as a female disinfectant product with weaker effects on the human body than pharmaceuticals.

1 is a view showing octenidine and isopropylmethylphenol, which are main components of a vulva cleaner composition for targeting antibacterial vaginitis according to embodiments of the present invention.

Figure 1a) shows the structure of octenidine according to the present invention, Figure 1b) shows the structure of isopropylmethylphenol according to the present invention.

Therefore, the vulvar cleaning composition for targeting antibacterial vaginitis according to embodiments of the present invention relates to a vulvar cleaning composition comprising octenidine, a naturally-derived phenolic compound, a foam forming agent, a foam holding agent, a deodorant, and purified water.

First, referring to FIG. 1, the vulva cleaning composition contains octenidine, a naturally-derived phenolic compound, and a foaming agent as active ingredients.

Here, the octenidine is octenidine dihydrochloride, which is a geminisurfactant having a Bolaamphiphiles structure that is strongly adsorbed on the cell surface of the cathode to form a phospholipid of the cell membrane. and reacts with the anionic polysaccharide of the cell wall of microorganisms, interferes with the enzyme system, interferes with cell function, resulting in leakage of the plasma membrane, and as a result, inhibits the function of mitochondria to exhibit excellent microbial sterilization effect. It shows synergistic effect with the forming agent to provide an effect of selectively sterilizing the gram-negative bacteria among gram-positive bacteria and gram-negative bacteria.

At this time, Gram-negative bacteria have a feature of having a thin peptide glycan cell wall between the inner cytoplasmic membrane and the outer membrane like a sandwich, and vaginitis, which is a general term for bacteria that cause vaginitis, corresponds to the Gram-negative bacteria.

Compared with the gram-negative bacteria, gram-positive bacteria have a characteristic of not having an outer membrane, and vaginal lactic acid bacteria that produce lactic acid in the vagina correspond to the gram-positive bacteria.

In addition, the naturally-derived phenol compound may include any one material or one or more materials selected from the group consisting of thymol, menthol, and isopropylmethylphenol.

The naturally occurring phenolic compounds may be prepared synthetically by known methods within the ability of a skilled person, or may be obtained from plant oil extracts.

In preferred embodiments of the present invention, the naturally-derived phenolic compounds may be obtained from plant extracts or commercially available products.

These naturally-derived phenolic compounds are characterized by being able to control the sterilizing range of the octenidine, and specifically, lower the sterilizing power of the octenidine against Gram-positive bacteria.

The foam forming agent includes one or more materials selected from among cocamidopropyl betaine, cocobetaine, sodium lauroyl oat amino acid, lauryl sulfate, polysorbate, and lauramine oxide.

The bubble forming agent is a natural surfactant and has a mild property that does not irritate the skin mucous membrane, gives skin irritation and increases viscosity. In addition, it has excellent cleaning power, foaming power, and thickening power, and has characteristics of having an antistatic effect and a conditioning effect.

The foam-forming agent can activate the sterilization effect of the octenidine and thus enhance the sterilization and disinfection effect. Bubbles are formed for a predetermined time so that

At this time, the preset time may be a holding time that satisfies a range of 10 to 20 minutes.

Specifically, the foam former serves to activate the sterilizing action of the octenidine, while inhibiting the sterilizing power of the octenidine, and more specifically, 100% by weight of the vulva cleaner composition With respect to, by exceeding 1% by weight, the bactericidal action against gram-positive bacteria is not activated, and only the bactericidal action against gram-negative bacteria can be activated.

Next, the vulva cleaning composition may further contain a foam retaining agent and a deodorant as auxiliary components.

The cell-retaining agent is glycerin, which is characterized by strong moisturizing properties and viscosity, and is widely used in general cosmetics.

The foam retaining agent is a colorless, transparent, and viscous liquid that has strong hygroscopicity and can homogeneously disperse the mixture. In addition, it can absorb moisture in the air, supply it to the skin, and protect the skin from external stimuli.

That is, the bubble retaining agent has the characteristics of suppressing the emission of water of the vulva cleaner, stabilizing the hardness and viscosity, and also serves to maintain the bubbles formed by the bubble forming agent for a long time.

Specifically, the bubble retaining agent prevents bubbles from quickly disappearing even when the bubble forming agent forms bubbles and the bactericidal action of octenidine against gram-negative bacteria is activated, thereby activating the bactericidal action of octenidine. keep for a long time

On the other hand, in the embodiments of the present invention, the bubble retaining agent may be contained in the vulva cleaning composition only when the vulvar cleaning composition contains the cocamidopropyl betaine as an active ingredient.

If the vulva cleaning composition contains sodium lauroyl oat amino acid by replacing comamidopropyl betaine as an active ingredient, it is preferable that the foam retaining agent is not contained.

The deodorant may include any one material selected from sugar cane extract, cypress tree extract, lemon extract, eucalyptus extract, and zeolite.

The deodorant can significantly reduce the fundamental cause of deteriorating marketability due to the irritating scent of the vulva cleaner through an excellent deodorizing effect.

In preferred embodiments of the present invention, sugarcane extract is used as the deodorant, and the sugarcane extract contains not only polycosanol, but also magnesium, calcium, vitamin B, and potassium, so it has anticancer action, moisturizing action, and moisture. Replenishment and aging skin vitality improvement effect is excellent. In addition, since the sugarcane extract reacts with odorous substances such as organic acids, flavone-based compounds, phenolic compounds and unsaturated aldehydes, and has an excellent chemical deodorizing effect, the unpleasant odor possessed by the vulva cleaning composition can be reduced.

As described above, the vulva cleaning composition for target antibacterial vaginitis according to embodiments of the present invention contains 0.01 to 5% by weight of octenidine, 0.01 to 1% by weight of a natural phenolic compound, and 1 to 10% by weight of a foaming agent. %, it may consist of 0 to 1.5% by weight of a bubble retaining agent, 0.05 to 0.15% by weight of a deodorant, and 90 to 97% by weight of purified water.

More specifically, when the content of the naturally-derived phenolic compound is increased, the bactericidal power of the octenidine is increased to kill both gram-positive bacteria and gram-negative bacteria. Natural-derived phenolic compounds should not exceed 1% by weight.

In addition, if the content of the foam-forming agent is lowered, the bactericidal power of the octenidine is greatly increased to kill even Gram-positive bacteria. to exceed %.

Hereinafter, the present invention will be described in more detail through examples, but it is obvious that the present invention is not limited by the following examples.

Examples 1 to 4: Preparation of vulva cleaning composition

In order to verify the effect of the weak skin irritation, odor and inflammation prevention and selective antibacterial activity of the vulvar cleaning composition, a vulvar cleaning composition was prepared, and the contents thereof are specifically shown in Table 1.

Example 1 Example 2 Example 3 Example 4 weight(%) weight(%) weight(%) weight(%) octenidine hydrochloride 0.2 0.2 0.2 0.2 thymol 0.1 0 0 0 menthol 0 0.1 0 0 isopropylmethylphenol 0 0 0.1 0.1 Cocamidopropyl Betaine 3 3 3 0 Sodium Lauroyl Oat Amino Acid 0 0 0 3 deodorant 0.1 0.1 0.1 0.1 glycerin One One One 0 Purified water 95.6 95.6 95.6 96.6

Comparative Example 1: Preparation of commercially available vulvar cleaning composition

A commercially available vulvar cleaner composition was prepared that is commercially available, does not contain octenidine, and contains only natural extracts and bactericidal components such as sodium benzoate.

Experimental Example 1: Sensory evaluation

In order to evaluate the efficacy of the vulvar cleaning compositions of Examples 1 to 3 and Comparative Example 1 of the present invention, sensory evaluation was performed on 20 female applicants regardless of age, and the results are shown in Table 2.

Women who agreed to this efficacy test usually felt a peculiar smell, but did not have any physical abnormalities, and they applied the compositions of Examples 1 to 3 and Comparative Example 1 twice a day, morning and evening, each time inside the vagina with their hands. After applying it externally, it was used for 1 week, respectively, by rinsing thoroughly.

Example 1 Example 2 Example 3 comparative example skin irritation no stimulation with mild irritation no stimulation slightly irritating smell weakness doesn't exist doesn't exist strong bubble holding time more than 15 minutes more than 15 minutes more than 20 minutes more than 5 minutes

In the sensory evaluation, referring to Table 2, Example 3 containing isopropylmethylphenol had no irritation and odor, and it was confirmed that bubbles were maintained the longest. In addition, Example 1 had a weak odor, and Example 2 had no odor, but it was confirmed that there was a slight irritation. In addition, looking at Examples 1 to 3, it can be seen that the retention time of bubbles is three times or more compared to Comparative Example 1.

Experimental Example 2: Evaluation of deodorizing power

In order to evaluate the deodorizing power of Examples 3, 4 and Comparative Example 1 of the present invention, 1 g of each of the compositions of Examples 3, 4 and Comparative Example 1 was administered to 5 g of amylase, and the results are shown in FIG. was

Figure 2 is a view comparing the deodorizing power of Examples 3 and 4 according to the present invention and Comparative Example 1.

As shown in FIG. 2, it can be confirmed that there is a difference in deodorizing power depending on the type of bubble forming agent, and it was confirmed that Examples 3 and 4 have improved deodorizing power than Comparative Example 1.

Experimental Example 3: Deodorization test evaluation

Examples 1 and 3 of the present invention were subjected to a deodorization test according to the KSI 2218 test method, and the results are shown in Table 3.

Referring to Table 3, it was confirmed that Example 3 showed a 95% odor reduction rate.

Experimental Example 4: Selective target antibacterial test evaluation

In order to examine whether Example 3 of the present invention selectively sterilizes Gram-negative bacteria including vaginitis, the ASTM E2315-16 test was conducted, and the results are shown in Table 4 and Figure 3.

At this time, Staphylococcus aureus was used as a substitute for Gram-positive bacteria, and Escherichia coli, which has a similar structure to vaginitis, was cultured and tested as a substitute for Gram-negative bacteria.

Gram-positive bacteria Initial bacterial count (bacterial count/mL) 2.6×10 ⁶ Number of germs after 15 seconds of contact (number of bacteria/mL) 1.2×10 ⁵ Common logarithmic (log) reduction 1.34 Bacterial reduction rate (%) 95.385 Gram-negative bacteria Initial bacterial count (bacterial count/mL) 1.9×10 ⁶ Number of germs after 15 seconds of contact (number of bacteria/mL) <10 Common logarithmic (log) reduction 5.28 Bacterial reduction rate (%) 99.999 ASTM E2315-16

Referring to Table 4 and FIG. 3, in Example 3, it can be confirmed that 99.99% of Gram-negative bacteria were killed within 15 seconds of contact time.

More specifically, after culturing (control) gram-positive bacteria and gram-negative bacteria in a beaker, the Example 3 was added and contacted for 15 seconds, and when the gram-positive bacteria and gram-negative bacteria were cultured again, the gram-positive bacteria were about 5% survived and re-divided to increase their number, and 99% of Gram-negative bacteria were eradicated.

This means that Gram-negative bacteria are selectively sterilized with the selective antibacterial activity of Example 3, in other words, that Example 3 has the efficacy of target antibacterial vaginitis. explained through 4.

Figure 4 is a view for explaining the principle of selective antibacterial activity of the vulva cleaning composition for targeting antibacterial vaginitis according to embodiments of the present invention.

4a) is a diagram showing the contact structure between Example 3 and Gram-positive bacteria, and FIG. 4b) is a diagram showing the contact structure between Example 3 and Gram-negative bacteria.

Referring to FIG. 4, isopropylmethylphenol, a naturally-derived phenolic compound of Example 3, has a limitation in sterilization because the octenidine hydrochloride is attached to it due to its OH- group and interferes with sterilizing the peptide glycan layer of Gram-positive bacteria. there is. However, since octenidine hydrochloride of Example 3 reacts with phospholipids of cell membranes without hindrance of naturally occurring phenolic compounds, only Gram-negative bacteria can be selectively and efficiently eradicated through this principle.

Experimental Example 5: fungicide test

To evaluate the fungal activity against Candida of Example 3 according to the present invention, the ASTM E2315-16 test was conducted, and the results are shown in Table 5.

fungicide test
(C. albicans) Bacterial reduction rate after 15 seconds of contact (%) 99.9 Bacterial reduction rate after 30 seconds of contact (%) 99.9 Bacterial reduction rate after contact for 60 seconds (%) 99.99 ASTM E2315-16

Referring to Table 5, in Example 3, it can be confirmed that Candida bacteria are 99.99% sterilized in 15 seconds.

Experimental Example 6: Antiviral test

For antiviral evaluation against herpes virus 1 of Example 3 according to the present invention, the ASTM E1052 test was conducted, and the results are shown in Table 6.

antiviral
(herpes virus 1) Concentration of inoculum solution 1.0×10 ⁵ Bacterial reduction value after 30 seconds of contact (log) 4.7 Bacterial reduction rate after 30 seconds of contact (%) 99.99 ASTM　E1052

Referring to Table 6, Example 3 was confirmed to have high bactericidal power against the herpes virus that causes herpes as a sexually transmitted infection.

Although the vulvar cleaner composition for targeting antibacterial vaginitis according to an embodiment of the present invention has been described as a specific embodiment, this is only an example and the present invention is not limited thereto, and the widest scope according to the basic idea disclosed herein should be interpreted as having A person skilled in the art may implement an embodiment that is not indicated by combining or substituting the disclosed embodiments, but this also does not deviate from the scope of the present invention. In addition, those skilled in the art can easily change or modify the disclosed embodiments based on this specification, and it is clear that such changes or modifications also fall within the scope of the present invention.

Claims (9)

In the vulvar cleaning composition for selectively targeting antibacterial vaginitis causing vaginitis,
It contains a naturally-derived phenolic compound composed of octenidine and isopropylmethylphenol and a bubble forming agent composed of cocamidopropyl betaine as active ingredients, and contains a bubble retaining agent and a deodorant as auxiliary ingredients,
The vulva cleaning composition,
Octenidine 0.01 to 5% by weight, natural phenolic compounds 0.01 to 1% by weight, foam former 2 to 9% by weight, foam retaining agent 0 to 1.5% by weight, deodorant 0.05 to 0.15% by weight and purified water 90 to 97% by weight made up of,
As the octenidine exhibits a synergistic effect with the naturally-derived phenolic compound and the foam-forming agent, it is attached to the outer membrane of the gram-negative bacteria among the gram-positive bacteria and gram-negative bacteria, which are the vaginitis bacteria, and selectively sterilizes the gram-negative bacteria,
The naturally-derived phenolic compound lowers the bactericidal power of the octenidine against the gram-positive bacteria to control the sterilization range of the octenidine,
The bubble forming agent forms bubbles so that the bactericidal action of the octenidine can be activated,
The bubble-retaining agent suppresses the dissipation of water of the vulvar cleaner composition and at the same time maintains the bubbles formed by the bubble-forming agent, the vulva cleaner composition.
According to claim 1,
The octenidine is
A vulvar cleaning composition that is octenidine dihydrochloride.
According to claim 2,
The naturally-derived phenolic compound,
A vulva cleaner composition comprising any one material or one or more materials selected from the group consisting of thymol and menthol.
According to claim 3,
The foam forming agent,
A vulva cleaner composition comprising any one or more substances selected from cocobetaine, sodium lauroyl oat amino acid, lauryl sulfate, polysorbate and lauramine oxide.
delete According to claim 4,
The foam retaining agent,
A vulvar cleanser composition that is glycerin.
According to claim 6,
The foam retaining agent,
The vulva cleaning composition contained in the vulvar cleaning composition only when the vulvar cleaning composition contains the cocamidopropyl betaine as an active ingredient.
According to claim 7,
The deodorant,
A vulva cleaner composition comprising any one material selected from sugar cane extract, cypress tree extract, lemon extract, eucalyptus extract, and zeolite.
delete

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