KR102481556B1 - Nozzle cap for specimen test container, and specimen test container and specimen test kit using same - Google Patents

Abstract

A nozzle cap for a sample test container that eliminates the complexity of manufacturing and is freed from the risk that the sample solution is used for measurement without being filtered during use due to removal of the retaining member or filter or misalignment of the filter, and sample test using the same Container and sample test kits are provided. The nozzle cap for a sample test container of the present invention can be connected to the open end of the container base to form a sample test container. a nozzle body having a cap portion connectable to the open end of the base of the container and a nozzle pipe for discharging the sample liquid from the dripping port; and a filter accommodated in the nozzle body, wherein the nozzle body has a tubular filter receiving portion inside the cap portion, and the filter is folded of a plurality of ribs provided at the open end of the filter receiving portion. It is accommodated and held in the filter accommodating portion by.

Description

Nozzle cap for sample test container, and sample test container and sample test kit using the same

The present invention relates to a nozzle cap for a specimen test container, and a specimen test container and specimen test kit using the nozzle cap.

A simple examination test capable of detecting infection of a pathogen such as a virus or bacteria, pregnancy, blood glucose level, etc. in a short time has been developed. Such simple inspection test kits do not require special equipment and can measure desired items at low cost through relatively simple operation.

In order to carry out such an inspection test, for example, a sample liquid collected from the pharynx or nasal cavity of a patient using a sampling tool, secretions such as nasal mucus, or part of excrement such as urine or feces are suspended in a buffer solution or the like in a sample test container. Prepare a sample sample solution. Analysis is performed by dropping the sample sample solution in the sample test container onto a predetermined position of the test plate included in the kit. For example, in order to remove unnecessary substances in the sample sample solution, the sample sample solution is placed in the sample sample container. filtered through a filter. The performance of this filtration greatly influences the performance of the analysis.

Here, an example of a conventional sample test container is shown. 9 is a diagram schematically showing an example of a specimen test container included in a conventional specimen test kit.

A conventional specimen test vessel 900 includes a container base 920 for accommodating the specimen sample liquid and a nozzle cap 940 for filtering and discharging the specimen sample liquid from the drip port 942 . The container base 920 is open at its upper side and has a threaded connection 924 on its outer surface. The connecting portion 924 is set in a shape capable of screwing with a screw thread (not shown) provided on the inner surface of the cap 944 below the nozzle cap 940 . Both the container base 920 and the nozzle cap 940 are molded from a thermoplastic resin such as polypropylene.

10 is a view schematically showing a nozzle cap 940 constituting the conventional sample test container 900 shown in FIG. 9, (a) is a longitudinal cross-sectional view of the nozzle cap 940, and (b) ) is an exploded longitudinal cross-sectional view of the nozzle cap 940.

As shown in (a) of FIG. 10 , the nozzle cap 940 is composed of a nozzle body 941 having a nozzle pipe 943 communicating with a drip port 942 and a cap portion 944 . The lower end of the nozzle body 941 is integrally molded with the cap portion 944, and the base end portion 948 of the cap portion 944 provided in a direction substantially perpendicular to the axial direction of the nozzle body 941 includes the cap portion ( 944), a filter 950 is inserted into a tubular filter accommodating portion 946 extending in a direction substantially perpendicular to the base end portion 948. In the filter accommodating portion 946, an inner cap 960 is also fitted or screwed from the outside, and the filter accommodating portion 946 and the inner wall 964 of the inner cap 960 are fixed. there is. In addition, a filter hole 962 designed to be slightly smaller than the outer diameter of the filter 950 is provided at the lower end of the inner cap 960 . In this way, the filter 950 is held and fixed by the proximal end 948 in the nozzle cap 940, the filter accommodating portion 946, and the inner cap 960. When the sample sample solution is dropped, the sample sample solution in the sample test container passes through the filter 950 and the nozzle tube 943 from the filter hole 962, and out through the drip port 942, for example, droplets. (液滴) is discharged in the form.

However, several problems remain in the conventional specimen test container 900 . For example, as shown in (b) of FIG. 10 , since the inner cap 960 is required as a holding member in addition to the filter 950, only the nozzle cap 940 is composed of at least three members. that needs to be configured. Also, for example, it is configured to hold the outer periphery of the filter 950 with the inner cap 960 of the retaining member. It is a point to avoid the risk that the sample liquid is used for measurement without being filtered during use due to omission of the inner cap 960 and/or filter 950 or misalignment of the filter 950 .

The present invention aims to solve the above problems, and its object is to eliminate the complexity of manufacturing, and to measure without filtering the specimen sample solution during use due to the removal of the holding member or filter or the filter deviation. It is to provide a nozzle cap for a specimen test container freed from the risk of being used, and a specimen test container and specimen test kit using the same.

The present invention is a nozzle cap that can be connected to the open end of the base of the container to form a sample test container,

a nozzle body having a cap portion connectable to the open end of the base of the container and a nozzle pipe for discharging the sample liquid from the dripping port; and

A filter accommodated in the nozzle body is provided;

here,

The nozzle body has a tubular filter receiving portion inside the cap portion, and

The filter is a nozzle cap that is accommodated and retained in the filter accommodating portion by folding a plurality of ribs provided at the open end of the filter accommodating portion.

In one embodiment, the plurality of ribs are provided at substantially equal intervals on the open end of the filter accommodating portion.

In one embodiment, the number of the plurality of ribs is 2 to 16.

In one embodiment, the nozzle body is made of a thermoplastic resin.

In another embodiment, retention of the filter by folding of the plurality of ribs is performed by ultrasonic treatment.

The present invention also provides a container for accommodating a specimen sample solution for performing a specimen test, comprising: the nozzle cap; and a container base for accommodating the specimen sample solution.

The present invention also relates to a container for accommodating the sample sample solution;

A specimen test kit including a test plate for analyzing the characteristics of an object to be measured included in the specimen sample solution.

In one embodiment, the sample test kit of the present invention further includes a sampling tool for collecting a sample.

ADVANTAGE OF THE INVENTION According to this invention, the complexity of manufacturing can be eliminated and manufacturing cost can be reduced. According to the present invention, the sample sample solution is freed from the risk of being used for measurement without being filtered during use, for example, due to omission of the holding member or filter or deviation of the filter, so that at the time of analysis with the sample sample kit, It is also possible to avoid erroneous dropping of unnecessary substances in the sample solution onto the test plate of the specimen sample kit. This makes it possible to detect the measured object more accurately in a short time.

1 is a diagram for explaining an example of a nozzle cap of the present invention, and is a schematic cross-sectional view of the cap.
Figure 2 is a bottom view of the nozzle cap of the present invention shown in Figure 1;
3 is a diagram schematically showing a nozzle cap constituting the sample test container of the present invention, and is a cross-sectional view of the nozzle cap and filter to explain a state before attaching the filter.
Fig. 4 is a schematic bottom view of the nozzle cap before attaching the filter shown in Fig. 3;
FIG. 5 is a view schematically showing some examples of the shape of the lip provided on the nozzle cap before attaching the filter shown in FIG. 3;
6 is a diagram schematically showing an example of a sample test container of the present invention, wherein (a) is a front view of the sample test container after attaching the nozzle cap shown in FIG. 1 to the base of the container, and (b) is a front view of the container It is a front view of the specimen test container explaining an example of a state before attaching the nozzle cap to the base.
Fig. 7 is a cross-sectional view of the sample test container of the present invention shown in Fig. 6 (b), schematically showing cross sections of the container base and the nozzle cap, respectively.
Fig. 8 is a diagram showing an example of a sample test kit of the present invention, in which (a) is a diagram schematically showing how a collected sample is added to a diluent at the base of a container, and (b) is a diagram showing the state in which a sample is added to a diluted solution at the base of a container. (c) is a diagram schematically showing how the sample test solution filtered through the sample test container in (b) is dropped onto the dropping portion of the desired test plate. .
9 is a diagram schematically showing an example of a specimen test container included in a conventional specimen test kit.
10 is a view schematically showing a nozzle cap constituting the conventional sample test container shown in FIG. 9, where (a) is a longitudinal cross-sectional view of the nozzle cap, and (b) is an exploded longitudinal cross-sectional view of the nozzle cap. .

Hereinafter, the present invention will be described using drawings.

(Nozzle cap for sample test container)

The nozzle cap for a sample test container (hereinafter referred to as "nozzle cap") of the present invention can be connected to the open end of the container base to form a sample test container. Here, the term "specimen test container" used in this specification refers to a container for accommodating various specimen sample solutions for conducting a specimen test.

1 is a view for explaining an example of a nozzle cap of the present invention, and is a schematic cross-sectional view of the cap.

As shown in FIG. 1, the nozzle cap 140 of the present invention is composed of a nozzle body 141 and a filter 150. In addition, the nozzle body 141 is composed of a nozzle pipe 143 and a cap portion 144. In the present invention, it is preferable that the nozzle tube 143 and the cap portion 144 are integrally molded for the purpose of eliminating the complexity of the manufacturing process.

The nozzle pipe 143 has a drip port 142 for discharging (eg, dripping) the filtered specimen sample solution. The nozzle tube 143 has a taper shape so that the diameter expands from the upper part to the lower part in the drawing, for example. The lower end of the nozzle pipe 143 may be in contact with the filter 150 or may be arranged at a predetermined interval. The shape of the drip port 142 is not necessarily limited, but is, for example, circular, and its outer diameter is preferably 1 mm to 5 mm, more preferably 2 mm to 4 mm. The length of the nozzle tube 143 is preferably 8 mm to 20 mm, more preferably 10 mm to 15 mm.

Inside the cap portion 144, there is a tubular filter accommodating portion 146 extending along the axial direction of the nozzle tube 143 (eg, substantially parallel to the axial direction of the nozzle tube 143). It is provided. The filter accommodating portion 146 preferably has a cylindrical shape. In this way, the drip port 142, the nozzle pipe 143, and the filter accommodating portion 146 communicate, and the sample sample liquid in the specimen test vessel is passed from the filter accommodating portion 146 through the nozzle pipe 143 to the drip inlet 142. ) can be released. In addition, the filter 150 is accommodated inside the filter accommodating portion 146 . The inner diameter of the filter accommodating portion 146 is preferably designed to match the outer diameter of the filter used or to be slightly smaller than the outer diameter of the filter. The inner diameter of the filter accommodating portion 146 is, for example, 2 mm to 15 mm, preferably 4 mm to 12 m. It is also preferable that the filter accommodating portion 146 is designed so that its diameter is slightly reduced toward the upper side of the drawing (ie, the side where the nozzle tube 143 is provided). This is to further improve the adhesion between the accommodated filter 150 and the inner wall of the filter accommodating portion 146.

The filter 150 is accommodated and held in the filter accommodating portion 146 by folding a plurality of ribs 149 provided at the open end 147 of the filter accommodating portion 146 . That is, in one embodiment of the present invention, the plurality of ribs 149 are integrally molded with the filter accommodating portion 146 on the open end 147 side of the filter accommodating portion 146, and the lip 149 As the end of the filter is directed toward the central axis of the filter accommodating portion 146, the filter 150 is accommodated and held in the filter accommodating portion 146 by the plurality of ribs 149. Here, the term “folding of a plurality of ribs” used in this specification means that, as shown in FIG. e.g., at an angle of approximately 90°); and a state in which all or part of the plurality of ribs integrally molded with the filter accommodating portion are melted to form a convex portion toward the central axis of the filter accommodating portion (for example, a plurality of ribs are melted and formed within the filter accommodating portion). the condition of holding the filter); And it says including the state in which these are combined. In the present invention, the filter 150 includes a filter accommodating portion 146, a plurality of lips 149, and the other end of the nozzle pipe 143 of the nozzle body 141 (an end opposite to the drip port 142) Corresponding to, it is preferably fixed between the base end 148 of the cap portion 144, and in the fixing, the filter 150, the filter receiving portion 146, the plurality of lips 149, and the base end It is more preferable that they are in contact with each other so that no gap is formed between them and (148).

Figure 2 is a bottom view of the nozzle cap of the present invention shown in Figure 1;

In the nozzle cap 140, a plurality of ribs 149 are preferably provided at approximately equal intervals on the open end 146 of the filter receiving portion 146. In the present invention, in a state where the lips 149 are disposed around the filter 150 at approximately equal intervals, preferably, they are folded toward the center of the filter accommodating portion 146, so that the periphery of the filter 150 is It can be held more evenly, and the stability of the filter 150 in the filter accommodating part 146 can be increased. Further, in the present invention, the number of ribs 149 provided on one nozzle cap 140 is not necessarily limited as long as they do not overlap and interfere with each other by folding, but is preferably 2 to 16. If the number of the lips 149 is one, there is a possibility that the filter 150 may not be properly held, and the stability of the filter 150 in the filter accommodating portion 146 may not be maintained. If the number of ribs 149 exceeds 16, adjacent ribs overlap and interfere with each other, resulting in not only obstructing the holding of the filter 150, but also causing more ribs 149 around the filter 150. will be covered When the filtration area of the filter 150 decreases, the filtration efficiency may decrease.

The nozzle cap 140 of the present invention is preferably made of, for example, a transparent material so that the presence or absence of the sample liquid in the contents can be visually recognized from the outside. Also, the nozzle cap 140 is preferably made of a plastic material such as a thermoplastic resin.

Examples of the thermoplastic resin include, but are not necessarily limited to, polypropylene, polyethylene, polyvinyl chloride, polyethylene terephthalate, and polystyrene. Polyethylene or polypropylene is preferable in terms of excellent chemical resistance and versatility. In the present invention, by using the above thermoplastic resin for the nozzle cap 140, it can be molded by, for example, injection molding, and the filter 150 is fixed by the plurality of lips 149, For example, it can be achieved by sonication as described below.

The material of the filter 150 accommodated in the nozzle cap 140 is not particularly limited, and a filter material employed in a specimen test container included in a conventional inspection test kit may be used.

A filter material is a material that can be used, for example, in microfiltration. Examples of the filter material include cellulose, glass fiber, silica fiber, nitrocellulose, cellulose ester, polyethersulfone, polysulfone, ethylene tetrafluoride resin, vinylidene fluoride resin, polycarbonate, polypropylene, polyamide, nylon 6,6, Polyester. cotton, and stainless steel fibers, and combinations thereof. The thickness of the filter 150 is not particularly limited, and any thickness may be selected by a person skilled in the art.

3 is a diagram schematically showing a nozzle cap constituting the sample test container of the present invention, and is a cross-sectional view of the nozzle cap and filter to explain a state before attaching the filter.

A drip port 242, a nozzle pipe 243, and a filter accommodating portion 246 are formed in advance by injection molding on the nozzle cap 240 before attaching the filter. The lip 249 is provided along the axial direction of the nozzle tube 243 above the open end 247 of the filter accommodating portion 246 (that is, in a direction substantially parallel to the axial direction of the nozzle tube 243). The open end 247 of the filter accommodating portion 246 preferably has a plane substantially perpendicular to the lip 249 (ie, a direction orthogonal to the axial direction of the nozzle tube 243).

FIG. 4 is a schematic bottom view of the nozzle cap 240 shown in FIG. 3 .

On the bottom surface of the nozzle cap 240, the base end 248 has, for example, a substantially flat plane in a direction orthogonal to the axial direction of the nozzle tube 243, and is in close contact with the filter accommodated, so that the filter is contained in the filter housing portion. can be fixed more appropriately. Further, the base end portion 248 is provided with a plurality of grooves 252 from the periphery of the filter accommodating portion 246 toward the end of the central nozzle tube 243 . The sample test solution filtered through the filter from the base of the container is collected in the nozzle pipe 243 through these grooves 252 and discharged to the outside through the dripping port 242. In addition, FIG. 4 describes a structure in which the base end 248 is flat so as to be in close contact with the filter and appropriately provided with grooves 252, but the present invention is not necessarily limited to this form. For example, it may have a structure in which a portion corresponding to the base end 248 slopes toward the nozzle tube 243 and a portion corresponding to one groove 252 becomes a rib and contacts and fixes the filter.

Fig. 5 is a diagram schematically showing several examples of the shape of the lip provided on the nozzle cap before attaching the filter shown in Fig. 3;

FIG. 5(a) is an enlarged view of the rib 249 circled by a broken line in FIG. 3 . The lip 249 is preferably formed in advance so that the angle 254 has an appropriately rounded shape in order to prevent interference due to overlap with an adjacent lip when holding the filter. The width (W ₁ ) and length (L ₁ ) of the lip 249 may be appropriately selected by a person skilled in the art according to the size (eg, diameter) of a filter to be used. For example, when using a filter with a diameter of 7 mm, the width W ₁ of the lip 249 is preferably 1 mm to 3 mm. The length L ₁ of the lip 249 is preferably between 1 mm and 3.5 mm. In the present invention, the shape of the lip 249 is not limited to that shown in FIG. 5 (a), and may have any shape, for example, as shown in FIG. 5 (b) to (d). can For example, (b) of FIG. 5 is an example of a lip 261 having a semicircular end, (c) is an example of a lip 262 having a trapezoidal shape that is short toward the end, and (d) is an example of a lip 263 having an approximately parallelogram shape.

Referring back to FIG. 3 , the filter 150 is inserted into the filter accommodating portion 246 provided with the plurality of ribs 249 until the end portion comes into contact with the proximal end portion 248 . After that, for example, ultrasonic treatment is performed on the portion of the lip 249 of the filter accommodating portion 246, and each lip 249 is folded to hold the filter 150. This ultrasonic treatment is performed using means known to those skilled in the art, and specific conditions necessary for ultrasonic treatment can be appropriately selected by those skilled in the art. Note that such ultrasonic treatment refers to an operation of melting the thermoplastic resin by applying ultrasonic vibration, for example, when the portion of the lip 249 is made of the thermoplastic resin.

In this way, the filter 150 is reliably accommodated and fixed in the filter accommodating portion 246 only by the nozzle cap 240 without using a holding member, thereby preventing, for example, the holding member or filter from falling off or the filter being misaligned. In case of use, the sample solution is not filtered and the risk of being used for measurement can be eliminated.

(sample test container)

6 is a diagram schematically showing an example of a sample test container of the present invention, where (a) is a front view of the sample test container after attaching the nozzle cap shown in FIG. 1 to the base of the container, and (b) is a front view of the container It is a front view of the sample test container explaining an example of a state before attaching the nozzle cap to the base.

As shown in (a) of FIG. 6, the specimen test container 100 of the present invention includes a container base 120 and a nozzle cap 140 for accommodating the specimen sample solution. When in use, the container base 120 and the nozzle cap 140 are combined with each other as shown in FIG. 6(a). In addition, the container base 120 is not particularly limited as long as it can be attached to the nozzle cap 140 .

6(b) is a front view of the specimen test vessel 100 of the present invention for explaining an example of a state before attaching the nozzle cap 140 to the vessel base 120. For example, the container base 120 has a cylindrical shape or a tapered shape so that the diameter decreases very slightly toward the bottom, and can accommodate, for example, 0.1 mL to 2 mL, preferably 0.4 mL to 1 mL of the sample sample solution. there is. The container base 120 also has a connection 124 that is open on its upper side and is threaded on its outer surface. The connecting portion 124 is set in a shape capable of being screwed with a screw thread (not shown) provided on the inner surface of the cap portion 144 at the bottom of the nozzle cap 140 .

Fig. 7 is a cross-sectional view of the specimen test container of the present invention shown in Fig. 6 (b), schematically showing cross sections of the container base and the nozzle cap, respectively.

As shown in FIG. 7, in the specimen test container 100 of the present invention, the container base 120 and the nozzle cap 140 are, for example, screw threads provided in the connection portion 124 of the container base 120, and the nozzle The cap portion 144 of the cap 140 is detachable by screwing with the screw thread of the connecting portion 145 provided on the inner surface of the cap portion 144 . In addition, when the nozzle cap 140 is attached to the base of the container 120, there is no leakage from the attached portion, and the sample test solution accommodated in the container 100 is discharged from only the drip port 142. It is preferable that sufficient adhesion is maintained between the connecting portion 124 of the base 120 and the connecting portion 145 of the nozzle cap 140 .

In addition, in the present embodiment, as described above, the case where the fixing form of the container base 120 and the nozzle cap 140 is by screw coupling between the connection part 124 and the connection part 145 has been described, but the present invention It is not limited to these forms. For example, it may be fixed by fitting the container base 120 and the nozzle cap 140 by a snap fit.

In the specimen test container 100 of the present invention, the container base 120 is also preferably made of, for example, a transparent material so that the presence or absence of the specimen sample liquid in the contents can be visually recognized from the outside. In addition, it is preferable that the container base 120 is also made of the same plastic material as, for example, the nozzle cap, such as a thermoplastic resin.

In the sample test container of the present invention, for example, when the container base is made of a thermoplastic resin, the container base can also be molded by injection molding.

In the sample test container of the present invention, a sample sample collected through a sampling device such as a cotton swab is added to a diluent such as a buffer solution in the base of the container, and a sample sample solution is prepared by mixing these. In the present invention, the diluent may be previously added to the base of the container and the opening sealed with an aluminum sheet or the like, which is opened during use, and the sample is added thereto. After that, the nozzle cap is tightly coupled to the container base by, for example, screwing. The sample sample solution prepared in this way is dropped into a test plate included in a sample test kit, for example, in a required amount through a drip port.

Examples of samples that can be accommodated in the sample test container of the present invention include collection fluids such as nasal cavity collection fluid and pharyngeal collection fluid; feces such as runny nose, sputum, feces, and urine; blood; Organization; and cells. In addition, examples of objects to be measured included in the specimen are not necessarily limited, but influenza virus, RS (Respiratory Synsytial) virus, adenovirus, group A hemolytic streptococcus, mycoplasma pneumoniae, legionella, human metapneumovirus, norovirus, rotavirus Pathogens such as viruses, sapoviruses, and adenoviruses for diarrhea, or substances such as proteins derived from the pathogens, or antibodies to them, peptide hormones, steroids, bioactive amines, vitamins, prostaglandins, tetracyclines, etc. Antibiotics, toxins produced by bacteria, etc., various tumor markers, pesticides, clinical markers such as nucleotides complementary to nucleic acid components derived from the pathogenic microorganisms, and allergic substances in foods and the like.

The sample test container of the present invention reliably accommodates and fixes the filter without using a holding member, so that the sample is not filtered during use and is used for measurement, for example, when the holding member or filter falls off or the filter is misaligned. It is a sample test container that can eliminate the risk of

(sample test kit)

The specimen test kit of the present invention includes a container for accommodating the specimen sample solution, and a test plate for analyzing the characteristics of the measured object included in the specimen sample.

The test plate constituting the present invention contains, for example, a capture substance and a detection reagent, and may be, for example, an immunochromatographic plate known in the art.

The capture material is, for example, when the specimen is a bacterium, virus, hormone, or other clinical marker, an antibody (eg, polyclonal antibody and monoclonal antibody) that specifically reacts and binds to them. can be heard This capture material may be immobilized on a predetermined membrane surface provided on a test plate by a method known to those skilled in the art. Immobilization of the trapping substance on the membrane is performed, for example, by adsorbing a solution obtained by diluting the trapping substance in a buffer or the like to the membrane, followed by drying. The capture material may be immobilized on the test plate, for example in the shape of a line.

For example, when the specimen test kit of the present invention is used as a kit for diagnosing disease caused by adenovirus and RS virus pathogens, anti-adenovirus antibody or anti-RS virus antibody is immobilized on the test plate as a capture material. . Alternatively, for example, when the sample test kit of the present invention is used as a detection kit for detecting a clinical marker or an allergic substance in food, the test plate has an antibody against the clinical marker or the allergic substance as a capture material. is fixed. The type of antibody that can be immobilized on one test plate is not particularly limited, and one or a plurality of antibodies may be immobilized.

The detection reagent is capable of specifically binding to an object to be measured and forming a complex with the object to be measured. For example, when the subject to be measured is an antigenic substance such as a virus, the detection reagent is an antibody against the virus, and a labeled antibody is exemplified. Examples of the antibody before labeling are the same as those that can be used for the above capture reagent. Examples of substances used for labeling include enzymes, fluorescent/chemiluminescent labels, magnetic labels, radioactive isotopes, colloidal gold, beads, and colored latex.

These detection reagents are incorporated in the test plate in advance by a method well known to those skilled in the art. For example, when the sample test kit of the present invention is used as a kit for diagnosing the onset of adenovirus or RS virus, colloidal gold-labeled anti-adenovirus antibody or colloidal gold-labeled anti-RS virus antibody is included on the test plate as a detection reagent. is built in. Alternatively, for example, when the sample test kit of the present invention is used as a detection kit for detecting a clinical marker or an allergic substance in food, the test plate contains a detection reagent for the clinical marker or the allergic substance. Antibodies are built in. Whether one or a plurality of measurement items are provided in one test plate is not limited.

Further, in the specimen test kit of the present invention, the detection reagent may be added to the test plate after the specimen test solution is added, instead of being incorporated in the test plate in advance.

The sample test kit of the present invention may further include a sampling tool for collecting a sample. Examples of such sampling tools include cotton swabs, sampling tools, sampling containers, brushes, and the like. It is preferable that the sampling tool is sterilized. The sample test kit of the present invention may further contain, if necessary, a reaction stopper for stopping the reaction between the enzyme and the substrate, for example. As such a reaction stop liquid, citric acid, sulfuric acid, etc. are mentioned, for example. In addition, the sample test kit of the present invention may contain handling/use instructions and the like as needed.

Next, as an example of a method of using the sample test kit of the present invention, when the kit is an influenza kit, and a nasal cavity sample is used as a sample, an example of a method of use will be described.

First, as shown in (a) of FIG. 8 , for example, a cotton swab 410 (collection tool) wiping the nasal cavity of a test subject in a predetermined pattern is immersed in a container base 120 containing a buffer solution 412. do. After that, the cotton swab 410 is moved up and down in the buffer solution 10 times or more, for example, and finally taken out by sweeping. In this way, a specimen sample solution is prepared in the container base 120. Then, as shown in (b) of FIG. 8, the container base 120 and the nozzle cap 140 are screwed together firmly while the specimen sample liquid 420 remains in the container base 120. . Then, as shown in (c) of FIG. 8, the container base 120 is pressed from both sides, and the specimen sample solution filtered from the dripping port 142 of the specimen test container 100 to a filter (not shown) ( A predetermined amount (eg, 3 drops) of 422 is dropped onto the drop portion 432 of the test plate 430 . Thereafter, the test plate 430 is left in a horizontal state, and after a predetermined time elapses, the display window 434 of the test plate 430 is visually determined by doctors. Through a predetermined line displayed on the display window 434, doctors can be positive or negative for various influenza viruses (eg, positive for influenza A virus antigen, positive for influenza B virus antigen, influenza A virus antigen and A determination of positive for either of the influenza B virus antigens, negative for both of the influenza A virus antigens and influenza B virus antigens, or retesting without appropriate display) can be made.

According to the present invention, for example, it is useful in the medical field where rapid and efficient simple examination is required without reducing the sensitivity and performance of analysis.

100 sample test container
120 Courage donation
140, 240 nozzle cap
141 nozzle body
142, 242 loading port
143, 243 nozzle tube
144 cap part
146, 246 filter receiving part
147, 247 open end
148, 248 base
149, 249 lip
150 filter
252 home
410 cotton swabs
412 buffer
420 sample sample solution
422 Filtered Sample Sample Solution
430 test plate
432 unloading
434 display

Claims (8)

A nozzle cap that can be connected to the open end of the base of the container to form a sample test container,
a nozzle body having a cap portion connectable to the open end of the base of the container and a nozzle pipe for discharging the sample liquid from the dripping port; and
And a filter accommodated in the nozzle body,
Here, the nozzle body has a tubular filter accommodating portion having a proximal end provided with a plurality of grooves extending from the periphery toward the end of the nozzle tube inside the cap portion,
the filter is accommodated and held between the lip in the filter receiving portion and the proximal end by folding a plurality of ribs provided at the open end of the filter receiving portion;
The ribs are provided at equal intervals on the open end of the filter accommodating portion,
The nozzle body is made of a thermoplastic resin,
Retention of the filter by folding of the lip is performed by ultrasonic treatment,
By folding the lip, the filter is fixed so that the end of the filter and the proximal end of the filter receiving portion are aligned on the same plane,
The lip and the groove are arranged to face each other with the filter interposed therebetween along a vertical cross-sectional direction of the nozzle body, and
The nozzle cap is designed so that the width of the lip is wider than the width of the groove. delete The nozzle cap according to claim 1, wherein the number of the plurality of ribs is 2 to 16. delete delete A container for accommodating a sample sample solution for performing a sample test,
The nozzle cap according to claim 1 or 3; and
A container having a container base for accommodating a specimen sample solution. A container for accommodating the specimen sample solution according to claim 6;
A specimen test kit comprising a test plate for analyzing the characteristics of an object to be measured included in the specimen sample. The sample test kit according to claim 7, further comprising a sampling tool for collecting a sample.

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