KR102480821B1 - Food composition for prevention of respiratory diseases from particulate matter - Google Patents

Abstract

The present invention is a respiratory disease from fine dust, containing bellflower and deodeokgwa, red ginseng, astragalus, angelica, soja, gaeja, pulmondong, golden, dermis, bokryeong, gyeji, peony, baekchul, locust, jujube, health and licorice as active ingredients. It relates to a food composition for prevention, and a method for preparing the same.
The food composition of the present invention facilitates the airway, so that waste is well communicated and breathes evenly, reinforces lung sound to facilitate the discharge and removal of phlegm, and reduces coughing, thereby reducing air pollution such as fine dust, cigarette smoke, fumes, etc. It is a food that improves and strengthens the protection of the respiratory system against pollutants. The present invention is safe and effective while helping to suppress the occurrence of respiratory diseases by increasing the resistance to fine dust or air pollutants in the respiratory tract and strengthening the body's own immunity by taking it like a vitamin supplement rather than post-mortem respiratory disease treatment. It is useful as an agent for improving and reinforcing respiratory protection.

Description

Food composition for preventing respiratory diseases from fine dust {FOOD COMPOSITION FOR PREVENTION OF RESPIRATORY DISEASES FROM PARTICULATE MATTER}

The present invention relates to a food composition, and more particularly to a food composition for preventing respiratory diseases from fine dust.

Recently, due to the development of industrial economy, environmental pollution and changes in dietary life, respiratory diseases are rapidly increasing. The increase in mortality and morbidity due to air pollution is one of the major risk factors for human health, and the World Health Organization has reported that 3 million people die every year due to air pollution (about 5% of all deaths), It is known that these effects on the human body are mainly caused by fine dust generated during the combustion of fossil fuels.

Fine dust (particulate matter) is a particulate complex contaminant having various sizes, compositions, and origins. It is generally dust suspended in the air. Dust with a diameter of 10 ㎛ or less is called fine dust (PM ₁₀ ), and 2.5 ㎛ or less is called fine dust (PM 10). Dust is referred to as ultra-fine dust (PM _2.5 ), and smaller than 0.1 ㎛ is referred to as ultra-fine dust (PM _0.1 ). The causes can be divided into artificial sources emitted during the combustion of fossil fuels such as coal or oil, and sources caused by the natural environment itself, such as yellow sand, volcanic ash, and forest fires. It is known that the components of fine dust are very complex and diverse, and the risk to health varies depending on the size, surface area and chemical composition of the particles.

In particular, fine dust in the earth is highly related to respiratory diseases, and causes pulmonary inflammatory reactions and worsening respiratory symptoms in the short term, increased drug use, hospital admissions and increased mortality, and in the long term, increased lower respiratory tract symptoms, decreased lung function, and asthma. and exacerbations and lung cancer in patients with chronic obstructive pulmonary disease (COPD).

In recently published literature on fine dust, it was confirmed that fine dust deteriorates lung function, and the rate of decline in lung function increases with long-term exposure. In addition, as the concentration of fine dust PM ₁₀ increases for chronic obstructive pulmonary disease, the number of patients hospitalized with COPD increases, proving the relevance. As for asthma, significant results were confirmed in the concentration of fine dust PM _2.5 , and statistical significance was confirmed in the occurrence of asthma in adults for long-term exposure. As the relationship between fine dust and various diseases such as interstitial lung disease and idiopathic pulmonary fibrosis is continuously studied, there is also a need for materials that can alleviate respiratory and lung damage and inflammation caused by fine dust. is believed to increase

Unlike the respiratory and lung damage caused by the inflow of bacteria or temporary poisoning and other components of the respiratory tract, fine dust cannot be eradicated by human immunity, and there is no way to forcibly discharge fine dust when it enters through the respiratory tract. Since it is not known exactly whether a problem will occur, new treatments and conventionally There is an urgent need for a food composition that can be used by the general public.

Examples of respiratory diseases include colds, bronchitis, pneumonia, tuberculosis, lung cancer, and chronic bronchitis. A cold is a disease in which mucus and nasal congestion suddenly occur in all strata, bronchitis is an acute bronchitis when a viral infection in the upper respiratory tract spreads to the lower respiratory tract, tuberculosis is an infectious disease caused by infection with Mycobacterium tuberculosis, and pneumonia is Chronic bronchitis is an inflammatory disease that occurs in the lung tissue, and chronic bronchitis refers to a disease in which the bronchial mucosa is narrowed by a fibrotic inflammatory reaction due to inflammation of the capillary bronchi and the airway is closed due to increased secretion of mucus from mucosal cells.

Chronic bronchitis is characterized by chronic cough, phlegm, and dyspnea during exercise. The sputum is usually white or slightly yellow in mucus and tends to come out a lot in the morning. If the disease progresses, shortness of breath gradually worsens over several months to years, and you feel shortness of breath even with slight activity. Causes include allergic, infectious, and traumatic, and pathologically, there are catarrhal, purulent, ulcerative, and infiltrative. The cause is most often caused by infection with bacteria, viruses, fungi, etc., and even people who are not normally infected are susceptible to infection when the resistance of the whole body is weakened.

The allergic reaction of allergic rhinitis is a kind of antigen and antibody hypersensitivity reaction, histamine is released from the cell membrane of mast cells and basophils, arachidonic acid is released, and cyclooxygenase (COX) and 5-lipoxygenase are released. (5-lipoxygenase, 5-L0) produces prostaglandins and leukotrienes, mediating the early response between 2 and 90 minutes after antigen exposure and the late response after 4 to 8 hours. The initial response is mainly caused by mediators, and the late reaction is mainly caused by cell invasion. In addition, both allergic and non-allergic rhinitis act as risk factors for the development of asthma. Allergic rhinitis is treated with desensitization when the antigen is clear, and other drug therapies, surgical therapies, physiochemical therapies, etc., but it is difficult to completely heal.

As described above, respiratory diseases such as asthma, allergic rhinitis, cough, sputum, acute and chronic bronchitis have some differences in their causes and symptoms, but have a common aspect in terms of inflammatory diseases, and currently used drugs Because airway dilators are not effective for inflammation that aggravates the disease and simply relieve symptoms, drug resistance occurs when used for a long time, and there is a risk of worsening the disease.

Therefore, it is necessary to develop a new composition capable of fundamentally preventing the cause and effectively improving symptoms by overcoming the limitations of these current drug treatments.

In the following, the prior art for food compositions for preventing respiratory diseases from fine dust is described.

Korean Patent Registration No. 10-2058618 relates to a composition for preventing or treating respiratory diseases induced by fine dust using plant extracts as an active ingredient, comprising a mixture containing black bean, pogongyeong, gold silver flower, sambaekcho, Cassia tora, and licorice. It relates to a composition for preventing or treating respiratory diseases that contains the extracted extract as an active ingredient, thereby alleviating inflammation of the respiratory tract and lungs caused by fine dust.

Korean Patent Publication No. 10-2019-0109278 relates to a herbal composition for preventing or treating respiratory diseases, comprising a mixture of two or more extracts selected from the group consisting of ginseng extract, ginseng extract, honeysuckle extract, and perilla japonica extract. It relates to a pharmaceutical composition for preventing or treating respiratory diseases and a food composition for preventing or improving respiratory diseases, including as an active ingredient.

Korean Patent Registration No. 10-1943475 relates to a food composition for preventing and improving respiratory diseases, containing as an active ingredient a mixed extract of a mixture of bellflower, deodeok, red ginseng, and other medicinal herbs, breathing through lung synergism. Enhances strength, prevents dry cough and phlegm due to lack of lung sound due to fine dust, abnormal climate and environmental factors, increases energy when taken when the body is emaciated and has no energy, and prevents chronic respiratory diseases by supplementing the lungs and compositions that can be treated.

Korean Patent Publication No. 10-2017-0115852 relates to a pharmaceutical composition for preventing or treating respiratory diseases containing a mixed herbal medicine extract, and more specifically, contains a mixed extract of citron, ginseng, bellflower and pear as active ingredients, It relates to a pharmaceutical composition exhibiting an effect of inhibiting respiratory inflammation and fibrosis.

Korean Patent Registration No. 10-0970739 relates to a composition for the prevention and treatment of respiratory diseases containing Barberry extract as an active ingredient, and in detail, by confirming that it exhibits expectorant activity, antitussive activity and antihistamine activity, It relates to a composition that can be used as a pharmaceutical composition useful for the prevention and treatment of and a health functional food.

However, in the prior art, from fine dust with bellflower and deodeokgwa, red ginseng, astragalus, angelica, soja, gaeja, mulberry, gold, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice as active ingredients. There is no teaching about food compositions for the prevention of respiratory diseases.

Accordingly, the present inventors smooth the airway to ensure that waste is well communicated and breathe evenly, reinforces lung sound to facilitate discharge and removal of phlegm, and reduces coughing, thereby reducing air pollution such as fine dust, cigarette smoke, and soot In response to the substance, the present invention was completed by confirming a food composition that protects, improves and strengthens the respiratory system.

An object of the present invention is to prevent respiratory diseases from fine dust containing only natural materials such as bellflower and deodeok as active ingredients, which protect, improve and strengthen the respiratory system in response to air pollutants such as fine dust, cigarette smoke, and fumes. It is for providing a food composition.

In addition, an object of the present invention is to provide a method for preparing a food composition for preventing respiratory diseases from fine dust containing only natural ingredients such as bellflower and deodeok as active ingredients.

In order to achieve the above object, the present invention uses bellflower and deodeokgwa, red ginseng, astragalus, angelica, soja, gaeja, pulmondong, golden, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice as active ingredients. To provide a food composition for preventing respiratory diseases from fine dust, and a method for preparing the same.

The food composition of the present invention facilitates the airway, so that waste is well communicated and breathes evenly, reinforces lung sound to facilitate the discharge and removal of phlegm, and reduces coughing, thereby reducing air pollution such as fine dust, cigarette smoke, fumes, etc. It is a food that improves and strengthens the protection of the respiratory system against pollutants. The present invention is safe and effective while helping to suppress the occurrence of respiratory diseases by increasing the resistance to fine dust or air pollutants in the respiratory tract and strengthening the body's own immunity by taking it like a vitamin supplement rather than post-mortem respiratory disease treatment. It is useful as an agent for improving and reinforcing respiratory protection.

One aspect of the present invention is fine dust, containing bellflower and deodeokgwa, red ginseng, astragalus, angelica, soja, gaeja, mulberry, gold, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice as active ingredients Provides a food composition for preventing respiratory diseases from. More specifically, the present invention is steamed and dried bellflower and deodeokgwa, red ginseng, astragalus, angelica, soja, gaeja, pulmondong, golden, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice. Prevention of respiratory diseases from fine dust, characterized in that obtained by mixing medicinal materials, adding the same amount of pears to the mixture, adding distilled water thereto and extracting, recovering the supernatant with filter paper, and preparing an extract by concentrating it under reduced pressure. It provides a food composition for

The food composition of the present invention may be, for example, blue. Cheong is obtained by mixing the obtained extract with materials such as honey, and is a form of food composition that can be consumed in a certain amount using a spoon or the like and is easy to store and take.

In addition, another aspect of the present invention has bellflower and deodeokgwa, red ginseng, astragalus, angelica, soja, gaeja, pulmondong, golden, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice as active ingredients, It provides a method for preparing a food composition for preventing respiratory diseases from fine dust.

More specifically, the manufacturing method of the present invention

Finely pulverize steamed and dried bellflower and deodeok, and dry herbs including red ginseng, astragalus, angelica, soja, gaeja, mulberry, golden, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice 6: 4:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1 to 10:5:1:1:1:1:1:1: preparing a mixture by mixing in a weight ratio of 1:1:1:1:1:1:1:1:1:1;

Add the same amount of pears to the mixture, add 5 to 10 times the amount (w/v) of distilled water to the pears and the mixture, extract at 80 to 140 ° C for 10 to 24 hours, repeat twice, filter paper and recovering the supernatant and concentrating it under reduced pressure to obtain a plant composition.

First, the active ingredient of the food composition of the present invention will be described as follows.

Bellflower ( Platycodon grandiflorum A. de Candolle (Campanulaceae)) is a natural food high in fiber and rich in vitamins and minerals. It has a unique bitter taste and aroma and is used in various dishes. It is a natural food known to help relieve asthma.

Deodeok ( Codonopsis lanceolata ) is rich in saponin, so it has a bitter taste and has a very strong inherent scent. It is a plant that has various effects such as relieving bronchial diseases, preventing arteriosclerosis, and controlling blood sugar.

Red ginseng (紅蔘) is a herbal medicine recipe made by steaming and drying ginseng (人蔘, wild ginseng). , it is a natural ingredient known to be beneficial for nourishment and tonic and has antioxidant activity.

Astragalus ( Astragalus membranaceus ) is a plant belonging to the legume family and is one of the herbs widely used in oriental medicine. It is known to be effective in chronic fatigue, loss of appetite, anemia, wound healing, fever, allergy, uterine bleeding, and uterine prolapse. It is a material.

Angelica gigas is the root of the perennial aromatic herb Angelica gigas belonging to the Apiaceae family and is a representative medicinal plant.

Soja is perilla frutescens ) as a medicinal material from oriental medicine, which is known to be effective in removing phlegm and treating respiratory tract diseases such as cough.

Gae is mustard of the cruciferous family ( Brassica juncea Czern. et Coss : 芥子), a medicinal material known to have gastric mucosal stimulation, local skin stimulation, and skin fungus inhibition.

McMoondong ( Liriope platyphylla ) is a monocotyledonous perennial plant of the liliaceae family, and is known to have antioxidant action, blood flow promotion, heart contraction force increase, sedation, immunity enhancement, blood sugar lowering, and antibacterial action.

Golden ( Scutellariae Radix ) is a rotten grass ( Scutellariae It is the root of baicalensis Georgi (Lamiaceae Labiatae)) as it is or with the pericarp removed.

The dermis is an orange of the rue family ( Citrus unshiu Markovich ) or the mature rind of a related plant of the same genus, essential oils stimulate digestive organs, promote digestion, expectorate, anti-ulcer, anti-gastric secretion, cardiac, increase blood pressure, anti-allergy, promote bile secretion, inhibit uterine smooth muscle, and have antibacterial action It is a natural material known to have

Bokryeong is a medicinal material made by removing almost the outer layer with the sclerotium of Poria cocos Wolf , a parasitic species on the roots of pine trees. It is a medicinal substance known to have , immune enhancing action and antitumor action.

Gyeji is a medicinal material made using the young branches of Cinnamomum cassia Blume or other plants related to the same genus. It is a known medicinal herb.

Peony ( Paeonia lactiflora ) is a dicotyledonous perennial plant belonging to the genus Peony and peony. It shows analgesic effect on gastroenteritis and spastic pain in the stomach, and is effective when there is abdominal pain, diarrhea, or flatulence due to digestive disorders. It is a medicinal herb that is known to be effective even when it is present.

Baekchul is a shovel of Asteraceae ( Atractylodes japonica Koidzumi ) or Baekchul ( Atractylodes macrocephala Koidzumi ) rhizome or pericarp is removed and dried, and it is excellent for chronic indigestion, enteritis, diarrhea, and appetite enhancement because it has stomach and digestive effects. It is effective, and is known to be widely used in combination with Wiryeongseon (威靈仙) and Banggi (防己) for chronic joint rheumatism.

Nabokja is a cruciferous radish ( Raphanus sativus L.) or the seeds of plants of the same genus, and is known to have staphylococcus aureus suppression action, skin fungus inhibitory action, blood pressure lowering action, and anti-inflammatory action.

Jujube is the fruit of the jujube tree and is a natural material used as a diuretic, tonic, and laxative.

Health is Ginger officinale Roscoe ), which refers to the dried rhizome, which promotes secretion of gastric juice, activates intestinal peristalsis, promotes digestion, relieves vomiting, excites the heart, increases blood pressure, and promotes blood circulation. It is a natural ingredient known to have anti-inflammatory, analgesic and antibacterial properties.

Licorice ( Glycyrrhiza uralensis ) is a dicotyledonous perennial plant belonging to the rose order legume family, and is the most used medicinal herb in oriental medicine.

Here, the active ingredients of the food composition, bellflower, deodeok, red ginseng, astragalus, angelica, soja, gaeja, pulse, golden, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice are 6:4:1: 1:1:1:1:1:1:1:1:1:1:1:1:1:1:1 to 10:5:1:1:1:1:1:1:1:1: Mixed in a weight ratio of 1:1:1:1:1:1:1:1, more preferably 6:4:1:1:1:1:1:1:1:1:1:1: It is mixed in a weight ratio of 1:1:1:1:1:1. The mixing ratio is the optimal ratio to exhibit the effect of the food composition of the food composition of the present invention, that is, the effect of preventing respiratory diseases from fine dust. represents a minor disadvantage.

In the present invention, the extract is preferably, the same amount of pears based on this mixture is added to the mixture, 5 to 10 times the amount (w / v) of distilled water is added to the mixture based on the pears and the mixture, and 80 to 140 It was obtained by heating at ° C. for 10 to 24 hours and extracting twice or more. When extracted at a temperature higher than this, the active ingredient may be destroyed, and when extracted at such a temperature, an extract can be obtained without destroying the active ingredient.

On the other hand, as described above, dry medicinal materials including red ginseng, astragalus, angelica, soja, gaija, mamoondong, golden, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice are used as fermented dry medicinal materials. it is desirable to be Alternatively, the fermented dry medicinal material is characterized in that it is obtained by pulverizing the medicinal material and fermenting it at a temperature of 36 ° C. for 72 hours in a fermentation broth consisting of rice water, sugar, fermented yeast and sun salt. This fermentation is performed together with fermenting bacteria, and it is preferable to use fermenting bacteria including Lactobacillus acidophilus, Bulgaricus fermentum, and Lactococcus lactis as the fermenting bacteria used at this time. As an example, a bacterial complex product developed by GL Bio Co., Ltd. can be used as such a fermenting bacteria. It does not use any chemical additives other than these fermenting bacteria, can shorten the fermentation start time by 1/3 compared to general fermenting bacteria, can shorten the total fermentation time by more than 50%, and the ratio of fermenting bacteria is 0.1% of the total amount. When used in a ratio of, the extraction yield of the active ingredient can be greatly increased from about 50% to 80% or more, and it is expected that the efficacy will be further increased when taking a product that has undergone a fermentation process.

The present invention is not limited to the above extraction method, but includes, for example, cold extraction, ultrasonic extraction, reflux cooling extraction, and the like.

The mixed extracts of bellflower, deodeok, red ginseng, astragalus, angelica, soja, gaeja, corms, gold, dermis, bokryeong, gyeji, peony, baekchul, locust, jujube, health and licorice are extracted, fractionated, or purified (separated). , fractions) may be all extracts, fractions, purified products obtained in each step, their dilutions, concentrates, or dried products.

In a preferred embodiment of the present invention, the extract may be included in an amount of 0.00001 to 15.0% by weight, more preferably 0.0001 to 10% by weight, and most preferably 0.0001 to 5% by weight based on the total composition.

The food composition of the present invention is a mixed extract of bellflower, deodeok, red ginseng, astragalus, angelica, soja, gaeja, pulse, golden, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice, It includes ingredients commonly added during food preparation, and includes, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavors. Examples of the aforementioned carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides such as common sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents (thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

For example, when the food composition of the present invention is prepared as a drink, bellflower, deodeok, red ginseng, astragalus, angelica, soja, gaeja, macmundong, golden, dermis, bokryeong, gyeji, peony, baekchul, lobokja, jujube In addition to extracts of , health and licorice, citric acid, high fructose corn syrup, sugar, glucose, acetic acid, malic acid, fruit juice, Eucommia extract, jujube extract, licorice extract, etc. may be further included.

As described in the following examples, the food composition of the present invention facilitates the airway, so that the waste is well communicated and breathes evenly, reinforces the lung sound to facilitate the discharge and removal of phlegm, and subsides cough, thereby reducing fine It is a food that protects, improves and strengthens the respiratory system in response to air pollutants such as dust, cigarette smoke and soot. The present invention is safe and effective while helping to suppress the occurrence of respiratory diseases by increasing the resistance to fine dust or air pollutants in the respiratory tract and strengthening the body's own immunity by taking it like a vitamin supplement rather than post-mortem respiratory disease treatment. It is useful as an agent for improving and reinforcing respiratory protection.

On the other hand, in the embodiment of the present invention, the cytotoxicity of the mixed extracts of bellflower, deodeok, red ginseng, astragalus, angelica, soja, gaeja, pulmondong, golden, dermis, bokryeong, gyeji, peony, baekchul, nubokja, jujube, health and licorice As a result of the confirmation, it was found that the extract was harmless to the human body. Therefore, the extract of the present invention has little toxicity and side effects, so it can be safely used even for long-term use, so it can be safely applied to food compositions.

Hereinafter, the present invention will be described in detail by the following examples.

However, the following examples are only to specifically illustrate the present invention, and the content of the present invention is not limited by these examples.

< Example 1> Preparation of the food composition of the present invention

The bellflower and deodeok were thoroughly washed with water and dried, and the bellflower and deodeok were steamed and dried for 12 hours.

The steamed bellflower root and deodeokgwa, red ginseng, astragalus, angelica root, soja, gaeja, mulberry, gold, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice 6: 4: 1: 1 by weight :1:1:1:1:1:1:1:1:1:1:1:1:1:1 was weighed, milled, and mixed to prepare a mixture.

The same amount of pears was added to the mixture, and 5 times the amount (w/v) of distilled water was added to the pears and the mixture, and extracted twice at 95° C. for 24 hours. The obtained composite extract was transferred to a concentrator, concentrated until it was in the form of starch syrup, honey was added and re-concentrated to make a food composition of the present invention according to 50 brix.

< comparative example 1>

A mixed extract was prepared in the same manner as in Example 1, except that the bellflower seeds were not used.

< comparative example 2>

A mixed extract was prepared in the same manner as in Example 1, except that the deodeok was not used.

< comparative example 3>

The mixing ratio of the bellflower deodeok red ginseng and other medicinal materials was 1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1 in a weight ratio of A mixed extract was prepared in the same manner as in Example 1 except that.

< comparative example 4>

The mixing ratio of bellflower, deodeok, red ginseng and other herbs was 5:5:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1 A mixed extract was prepared in the same manner as in Example 1, except for the weight ratio.

< Experimental example 1> Expectorant activity test

The present invention using the method of Engler et al. (Engler H, Szelenyi I, J. Pharmacol. Moth., 1984, 11, 151-157; Jian-Hua Shang et al, J. Ethnopharmacology, 2010, 129, 293-298) The expectorant activity of the mixed extracts of bellflower, deodeok, red ginseng, and other medicinal herbs was measured.

To this end, 10 male ICR mice (Orient Bio, Korea) weighing 30 to 33 g were used as experimental animals. The extract of the present invention and comparative drug ambroxol (250 mg/kg, ambroxol, Sigma) and ammonium chloride (500 mg/kg, ammonium chloride) were orally administered to mice fasted the previous day for each experimental group, and after 30 minutes, 5% Phenol red was injected intraperitoneally. After 30 minutes, the abdominal aorta was exsanguinated by cervical dislocation and the entire trachea was resected. The separated organs were placed in 1 ml of physiological saline and stored in a refrigerator. After 24 hours, the refrigerated organ was centrifuged at 3000 rpm for 10 minutes, and 1N caustic soda (NaOH) was added to the supernatant (1N NaOH 0.1 ml per 1 ml of supernatant), and then the absorbance was measured at 546 nm to obtain the concentration of phenol red. Expectorant activity was measured and the results are shown in Table 1 below.

Oral dose (mg/kg) Sputum discharge capacity (%) Example 1 100 10 200 28 400 45 Comparative Example 1 100 5 200 9 400 13 Comparative Example 2 100 4 200 8 400 14 Comparative Example 3 100 6 200 11 400 23 Comparative Example 4 100 4 200 12 400 18 Ambroxol 250 29 Ammonium Chloride 500 37

As shown in Table 1, the mixed extract of bellflower, deodeok, red ginseng, and other medicinal herbs of the present invention was found to have excellent expectorant activity in a dose-dependent manner, and the mixture without bellflower (Comparative Example 1) and deodeok In the case of the mixture (Comparative Example 2) that did not contain it, it was confirmed that the effect was very low, and even when the weight ratio of bellflower, deodeok, red ginseng, and other herbs was out of the scope of the present invention (Comparative Example 3 and Comparative Example 4) It was confirmed that the effect was not excellent.

< Experimental Example 2> Antitussive activity test

In order to evaluate the antitussive activity of the mixed extract of bellflower, deodeok, red ginseng and other medicinal herbs of the present invention (American Journal of Respiratory and Critical Care Medicine, 1998, 158 [1], 42-48), the extract of Example 1 was After administration to guinea pigs, citric acid was sprayed to induce coughing, and then the number of coughs was measured. Citric acid is a substance widely used to study airway sensory nerve activity, and when inhaled, bronchoconstriction, nasal irritation, coughing, bronchial hypersensitivity, etc. occur.

As experimental animals, 10 to 12 male Hartely guinea pigs (Orient Bio, Korea) weighing 350 to 400 g were used in each group. The mixed extract prepared in Example 2 and the comparative drug theobromine (50 mg/kg, theobromine, Sigma) were orally administered to guinea pigs fasted the previous day for each experimental group, and 1 hour later, 0.3M citric acid, a cough-inducing substance, was administered to the animals for 10 minutes. Spraying caused coughing. The number of coughs generated for 15 minutes from the start of exposure to cough-inducing substances was measured using a Whole Body Plethysmometer (BUXCO), and the suppression rate (%) was calculated based on the number of coughs in the negative control group (1% CMC administration group). It was calculated and shown in Table 2 below. Theobrobin was used as a comparative drug.

Oral dose (mg/kg) Cough suppression rate (%) Example 1 100 20 200 49 400 60 Comparative Example 1 100 2 200 5 400 13 Comparative Example 2 100 3 200 10 400 20 Comparative Example 3 100 4 200 9 400 15 Comparative Example 4 100 4 200 12 400 15 Theobrobin 100 15

As shown in Table 2, the mixed extract of bellflower, deodeok, red ginseng, and other medicinal herbs of the present invention was found to have excellent cough suppression rate in a dose-dependent manner, and the mixture without bellflower (Comparative Example 1) and deodeok In the case of the mixture (Comparative Example 2) that did not contain it, it was confirmed that the effect was very low, and even when the weight ratio of bellflower, deodeok, red ginseng, and other herbs was out of the scope of the present invention (Comparative Example 3 and Comparative Example 4) It was confirmed that the effect was not excellent.

< Experimental example 3> airway contraction inhibition test (in vivo )

It was confirmed whether the extracts of the herbal medicine mixtures prepared in Example 1 and Comparative Examples 1 to 4 had airway contraction inhibitory activity (Experiment method-respiratory volume measurement confirmation: European Journal of Pharmacology, 2000, 403, 169-179; Br. J (Pharmac., 1983, 78, 67-74).

To this end, Hartley male guinea pigs (400-450 g, SLC, Japan) were sensitized by intravenous injection of anti-ovalbumin antiserum at a volume of 1.5 ml/kg. The lyophilisate (400 mg/kg) of each extract was orally administered to guinea pigs 48 hours after sensitization. 30 minutes after oral administration, pretreatment was performed by subcutaneous injection of pyrilamine maleate (0.5 mg/kg) and propranolol (0.05 mg/kg). It was placed in a double chamber plethysmograph box (type 855, HSE, German) equipped with a plethysmometer, and basic airway resistance (RxV) was measured. After 30 minutes of pretreatment, 1% ovalbumin was made into an aerosol using high-pressure compressed air, sprayed for 2 minutes, and then air containing albumin was blown out of the chamber for 30 seconds. Airway resistance was measured in the plethysmometer box for 15 minutes, and airway resistance was calculated by AUC (area under the curve), and compared to the solvent control group, shown in Table 3 as airway contraction inhibition (%) .

Inhibition of airway contraction (%) Example 1 62 Comparative Example 1 15 Comparative Example 2 14 Comparative Example 3 20 Comparative Example 4 21

As shown in Table 3, the mixed extracts of bellflower, deodeok, red ginseng, and other medicinal herbs of the present invention were found to be significantly superior in airway contraction inhibitory activity compared to the extracts of Comparative Examples 1 to 4.

< Experimental example 4> Bronchial inflammation inhibition test

In order to confirm whether the extracts of the herbal mixtures prepared in Example 1 and Comparative Examples 1 to 4 have an inhibitory effect on lung bronchial inflammation in asthma and chronic obstructive respiratory disease models, the effect of exposing sensitized mice to antigens in the lung bronchus induced The leukocyte increase response was confirmed (Pharmacological Research, 2010, 61, 288~297; Biochemical Pharmacology, 2010, 79, 888~896).

To this end, BALB/c female mice (6.5 weeks old, SLC, Japan) were treated with 10 μg of ovalbumin (OVA, sigma) and aluminum hydroxide (4 mg, aluminum hydroxide, Alum, Pierce) 1:1. 0.2 ml of the mixed solution was intraperitoneally administered on days 0, 7 and 14 to sensitize. 8 and 10 days after the final sensitization day, 1.0% ovalbumin was made into an aerosol using high-pressure compressed air and sprayed for 50 minutes to induce airway inflammation. Administration of each test substance (400 mg/kg of lyophilisate of each extract, b.i.d.) was inoculated by oral administration twice a day in the morning and afternoon from the 21st to the 23rd after the first sensitization. 24 hours after the last inoculation, bronchoalveolar lavage was performed using 1.5 ml of phosphate buffer (pH 7.2), and the bronchoalveolar lavage fluid was collected. The number of leukocytes in the washing solution was counted using a hematology analyzer (Drew Scientific Inc., HEMAVET = HV950FS, M-950HV). The results are shown in Table 4 below by calculating the bronchial inflammation inhibition rate (%) based on the number of white blood cells in the negative control group (1% CMC [carboxymethyl cellulose] administration group).

Bronchial inflammation inhibition rate (%) Example 1 32 Comparative Example 1 5 Comparative Example 2 4 Comparative Example 3 10 Comparative Example 4 12

As shown in Table 4, it was found that the mixed extract of bellflower, deodeok, red ginseng, and other medicinal herbs of the present invention had an excellent effect of inhibiting the number of white blood cells in bronchoalveolar lavage fluid, and as a result, the extracts suppressed inflammation. I was able to confirm that it was excellent.

< Experimental example 5> Confirm asthma treatment

Each extract of Example 1 and Comparative Examples 1 to 4 was administered to patients in their 20s to 50s diagnosed with asthma at the hospital from May 2019, 7 people in each group, 110 ml 3 times a day for 20 days after meals 2 After taking it for 20 days, 5 points in Table 5 below (5 points: improvement effect of 80% or more, 4 points: improvement effect of 60% or more, 3 points: improvement effect of 40% or more, 2 points) : Improvement effect of 20% or more, 1 point: Improvement effect of less than 10%, 0 point: No improvement effect), indicating the improvement effect of cough and phlegm according to asthma symptoms.

Bronchial inflammation inhibition rate (%) Example 1 4.5 Comparative Example 1 1.0 Comparative Example 2 1.2 Comparative Example 3 2.0 Comparative Example 4 2.3 non-administration group 0

As shown in Table 5, the asthma treatment effect of the mixed extract of bellflower, deodeok, red ginseng and other medicinal herbs of the present invention was found to be excellent.

< Experimental example 6> Toxicity test

When an excessive amount of the extract (freeze-dried product) of bellflower, deodeok, red ginseng and other medicinal herbs of Example 1 of the present invention was consumed in a short period of time, the acute (within 24 hours) toxicity to the animal body was investigated, and the mortality rate was investigated. This experiment was conducted to determine. Thirty ICR mouse strains, which are common mice, were assigned to the control group and the experimental group, 10 each. In the control group, only PEG-400/Tween-80/EtOH (8/1/1, v/v/v) was administered, and in the experimental group, the extract of the crude drug mixture of Example 1 of the present invention was administered to the PEG-400/Tween-80 / EtOH (8/1/1, v/v/v) was dissolved and orally administered. As a result of examining each mortality rate 24 hours after administration, it was confirmed that all mice survived in the control group and the experimental group administered with the extract of the herbal medicine mixture of Example 1 of the present invention at a concentration of 2 g/kg/day.

Formulation examples for the composition of the present invention are exemplified below.

< formulation example 1> of health food Produce

Mixed extracts of bellflower, deodeok, red ginseng and other medicinal herbs of Example 1. 200 mg

Vitamin Blend ................................................ Moderate amount

Vitamin A Acetate ................... 70 μg

Vitamin E ........................................... 1.0 mg

Vitamin B1 ................................... 0.13 mg

Vitamin B2 ................................... 0.15 mg

Vitamin B6 ................................... 0.5 mg

Vitamin B12 ........................................... 0.2 μg

Vitamin C ................................................ 10 mg

Biotin ................................................ 10 μg

Nicotinamide ................................... 1.7 mg

Folic acid ................................................ 50 µg

Calcium Pantothenate ................................... 0.5 mg

Mineral Mixture ................................................ Moderate amount

Ferrous Sulphate ........................................... 1.75 mg

Zinc Oxide ................................. 0.82 mg

Magnesium Carbonate ................................... 25.3 mg

Potassium Phosphate Monobasic ................................... 15 mg

Dibasic Calcium Phosphate ................................... 55 mg

Potassium Citrate ................................... 90 mg

Calcium Carbonate ................................................ 100 mg

Magnesium Chloride ................................... 24.8 mg

Although the composition ratio of the above vitamin and mineral mixture was prepared by mixing ingredients suitable for relatively healthy food in a preferred embodiment, the mixing ratio may be arbitrarily modified. , Granules can be prepared and used in the preparation of health food compositions according to conventional methods.

< formulation example 2> Manufacture of health drinks

Mixed extract of bellflower, deodeok, red ginseng and other medicinal herbs of Example 1 ... 200 mg

Citric acid ................................................ 1000 mg

Oligosaccharides ................................................ 100 g

Plum concentrate .................................. 2 g

Taurine ................................................ 1 g

Add purified water ................... Total 900 ml

After mixing the above components according to the usual health drink manufacturing method, stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2-liter container, sealed and sterilized, and then refrigerated according to the present invention. It is used in the manufacture of health beverage compositions.

Although the composition ratio is a mixture of ingredients suitable for a relatively favorite beverage in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as the class of demand, the country of demand, and the purpose of use.

Claims (6)

As a food composition for preventing respiratory diseases from fine dust,
The food composition
Steamed and dried bellflower and deodeok, and
Forming a mixture by mixing fermented products obtained from dry medicinal materials including red ginseng, astragalus, angelica, soja, gaija, maekmundong, golden, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice;
add an equal amount of pears to the mixture;
distilled water in an amount (w/v) 5 times the amount of the mixture was added thereto;
It was extracted twice for 24 hours at 95°C;
recover the supernatant using filter paper;
preparing an extract by concentrating the recovered supernatant under reduced pressure until it becomes a syrup-like form;
The extract was mixed with honey and re-concentrated to obtain a sugar content of 50 Brix in the form of
Fermented products obtained from dry medicinal materials including red ginseng, astragalus, angelica, soja, gaeja, macmundong, golden, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice
Red ginseng, Astragalus, Angelica, Soja, Gaeja, Maekmundong, Hwanggeum, Dermis, Bokryeong, Gyeji, Peony, Baekchul, Nabokja, Jujube, Health and Licorice are respectively pulverized to obtain pulverized dry medicinal materials;
These pulverized dry medicinal materials were mixed with fermenting bacteria including Lactobacillus acidophilus, Bulgaricus fermentum, and Lactococcus lactis in a fermentation broth composed of rice water, sugar, fermented yeast, and sea salt at a temperature of 36° C. for 72 hours. It is obtained by fermentation
The weight ratio of the steamed and dried bellflower and deodeok, and dry medicinal materials including red ginseng, astragalus, angelica, soja, gaeja, mulberry, gold, dermis, bokryeong, gyeji, peony, baekchul, nabokja, jujube, health and licorice is 6 :4:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1 Food composition, characterized in that.
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