KR102469550B1 - Cosmetic composition with uv protection, whitening and wrinkle improvement effects - Google Patents

Abstract

The present invention relates to a cosmetic composition having UV protection, skin whitening and anti-wrinkle effects. According to one embodiment of the technical concept of the present invention, the cosmetic composition having UV protection, skin whitening and anti-wrinkle effects includes 1 to 5 wt% of zinc oxide, 2 to 5 wt% of niacinamide, 0.01 to 0.1 wt% of adenosine, and 2 to 6 wt% of Artemisia vulgaris oil as active ingredients. By including zinc oxide, niacinamide, adenosine and Artemisia vulgaris oil according to various embodiments of the technical idea of the present invention as active ingredients as in the above configuration, the composition has excellent UV protection, skin whitening, anti-wrinkle and skin barrier-improving effects and can enhance skin health.

Description

Cosmetic composition with UV protection and whitening and wrinkle improvement {COSMETIC COMPOSITION WITH UV PROTECTION, WHITENING AND WRINKLE IMPROVEMENT EFFECTS}

The present invention relates to a cosmetic composition having sunscreen and whitening and wrinkle-improving effects, and more particularly, by constructing a cosmetic composition containing zinc oxide, niacinamide, adenosine and vulgaris mugwort oil as active ingredients, thereby providing UV protection, whitening, It relates to a cosmetic composition having excellent skin wrinkle improvement and skin barrier improvement effects and UV protection and whitening wrinkle improvement effects that can improve skin health.

In modern society, a large amount of active oxygen is generated due to ultraviolet rays and water pollution due to exhaust gas, harmful chemicals, and destruction of the ozone layer. These active oxygen penetrate the human body and cause various side effects, especially pigment change and skin aging. are known to be the main causes of

In addition, in recent years, outdoor leisure activities such as golf are increasing in order to relieve excessive social and work stress. and skin aging are unavoidable, negatively affecting the trend to enjoy outdoor leisure activities more actively and comfortably.

In general, the skin is in charge of the primary function of preventing harm from various external factors that impede the homeostasis of our body through basic metabolic processes. In other words, as the skin is located at the outermost part of the body, it performs various roles such as protection through physical, chemical, and biological barrier functions, temperature regulation, sensation, secretion and excretion, absorption, storage, and regeneration. However, it is most susceptible to damage when subjected to sustained external stress.

Major causes of these external stresses include exposure to ultraviolet rays, exhaust gas, yellow dust, environmental pollutants, heavy metals, temperature changes, and microorganisms. These are mainly reactive oxygen species through oxidative stress. , ROS), thereby disrupting the antioxidant defense system and causing cell damage and death.

In general, skin wrinkles due to weakened elasticity and contractility of skin cells appear due to various causes, but it is widely known that skin aging reduces skin contraction and convergence.

Skin aging is largely divided into intrinsic aging and extrinsic aging. Intrinsic aging is a naturally occurring aging phenomenon in which the physiological function of the skin gradually deteriorates with age. Clinically, it is characterized by a decrease in elasticity, rough skin texture, deep wrinkles, and pigmentation.

In addition, aging caused by external factors refers to an aging phenomenon caused by extrinsic factors such as ultraviolet rays, reactive oxygen species, and stress. Aging of the skin progresses rapidly due to these factors, and as a result, rough wrinkles are formed on the skin surface and elasticity is significantly reduced.

Various studies have been conducted to identify the aging mechanism of the skin as described above, and various causes of skin aging have been identified through these studies. Aging is accelerated as the physiological activity of the skin as a barrier to defense decreases, and when the skin is exposed to harmful reactive oxygen species or free radicals, lipid peroxides are produced by oxidation in the body and various proteins constituting the skin are transformed, resulting in skin damage. Aging proceeds.

Such skin aging and loss of elasticity are caused by deterioration and destruction of collagen and elastin production due to weakening of skin cells (fibroblasts) by ultraviolet rays and the like. Collagenase and Elastase are expressed in the skin by ultraviolet rays, etc., and these enzymes decompose normal collagen and elastin in the skin, resulting in a decrease in skin elasticity.

On the other hand, the destruction of the ozone layer along with the rapid industrialization of modern society causes an increase in the amount of UV to generate strong oxidizing substances such as reactive oxygen species, and damages cell membranes and DNA, thereby accelerating skin aging. Furthermore, melanin, which is produced as a defense material against oxidants such as reactive oxygen species, induces pigmentation and causes melasma and freckles, and lipid peroxide due to cell membrane destruction is also a factor inducing skin trouble.

Therefore, since antioxidant activity is the most basic effect of protecting the skin, various antioxidants such as vitC, tocopherol, and coenzyme Q10 are used in cosmetics manufacturing to protect the skin from various oxidants.

Accordingly, the inventors of the present invention construct a cosmetic composition containing zinc oxide, niacinamide, adenosine, and vulgaris mugwort oil as active ingredients, so that the effects of sunscreen, whitening, skin wrinkle improvement, and skin barrier improvement are excellent and skin health can be improved. It was confirmed that there was, and the present invention was completed.

Korean Registered Patent No. 10-1477891 (registered on December 23, 2014) Domestic Patent No. 10-2052511 (registered on November 29, 2019) Domestic Patent No. 10-0966835 (registered on June 22, 2010)

The problem to be solved by the present invention is to construct a cosmetic composition containing zinc oxide, niacinamide, adenosine, and vulgaris mugwort oil as active ingredients, so that UV protection, whitening, skin wrinkle improvement and skin barrier improvement effects are excellent, and the skin It is to provide a cosmetic composition having UV protection and whitening and anti-wrinkle effects that can improve health.

In addition, another problem to be solved by the present invention is to minimize skin irritation, to make dry and dry skin moist and smooth, to have excellent skin absorption effect lasting for a long time, to have excellent formulation stability of cosmetics, to have excellent UV protection and whitening wrinkle improvement effect It is to provide a cosmetic composition with.

Various problems to be solved by the present invention are not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In one embodiment of the technical idea of the present invention, a cosmetic composition having UV protection and whitening and anti-wrinkle effects is disclosed.

The cosmetic composition having sunscreen and whitening and wrinkle-improving effects includes zinc oxide, niacinamide, adenosine, and vulgaris mugwort oil as active ingredients, and 1 to 5% by weight of the zinc oxide, 2 to 5% by weight of niacinamide, and 0.01 adenosine to 0.1% by weight and 2 to 6% by weight of vulgaris mugwort oil.

Details of other embodiments are included in the detailed description.

The cosmetic composition having sunscreen and whitening and wrinkle-improving effects according to various embodiments of the technical concept of the present invention contains zinc oxide, niacinamide, adenosine and vulgaris mugwort oil as active ingredients, thereby blocking ultraviolet rays, whitening, and improving skin wrinkles And it is excellent in skin barrier improvement effect and can improve skin health.

In addition, the cosmetic composition having UV protection and whitening and wrinkle improvement effects according to various embodiments of the technical idea of the present invention can minimize skin irritation, make dry and dry skin moist and smooth, and has an excellent skin absorption effect that lasts for a long time The formulation stability of cosmetics is excellent.

It will be fully understood that various embodiments of the technical idea of the present invention can provide various effects not specifically mentioned.

1 is a graph showing the skin brightness improvement effect of the cosmetic compositions according to Examples 1, 2 and 3 and the cosmetic compositions according to Comparative Examples 1 and 2.
Figure 2 is a graph showing the skin wrinkle improvement effect of the cosmetic compositions according to Examples 1, 2, and 3 and the cosmetic compositions according to Comparative Examples 1 to 4.

The advantages and features of the present invention, and how to achieve them, will become clear with reference to the detailed description of the embodiments below. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed content will be thorough and complete and the spirit of the present invention will be sufficiently conveyed to those skilled in the art.

Terms used in this application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having meanings consistent with the meanings in the context of the related art, and unless explicitly defined in this application, they should not be interpreted in ideal or excessively formal meanings. don't

Hereinafter, a cosmetic composition having UV protection and whitening and anti-wrinkle effects according to an embodiment of the technical idea of the present invention will be described in detail with a preferred embodiment.

According to one embodiment of the technical concept of the present invention, the cosmetic composition having sunscreen and whitening and wrinkle-improving effects includes zinc oxide, niacinamide, adenosine, and vulgaris mugwort oil as active ingredients.

In addition, the cosmetic composition having sunscreen and whitening and wrinkle-improving effects according to an embodiment of the technical idea of the present invention contains 1 to 5% by weight of zinc oxide, 2 to 5% by weight of niacinamide, 0.01 to 0.1% by weight of adenosine and vulgaris mugwort. Oil may be included in a weight ratio of 2 to 6% by weight.

The zinc oxide is a compound of oxygen and zinc made from powder purified by burning and heating an ore called zinc oxide in a natural state. It is insoluble in water and is a white inorganic oxide (zinc oxide) inactive mineral component. It is often used as a thickening agent, whitening agent, lubricant, and sunscreen agent.

The zinc oxide blocks UVA and UVB at the same time by reflecting sunlight, and has a function of preventing skin cell damage and preventing skin aging by scattering visible rays. When used on the skin, it has an immediate blocking effect and has the advantage of being more stable than chemical sunscreens.

Niacinamide is a vitamin B complex that is essential to the human body and is included in foods such as green vegetables and grains. In addition, it is stable to heat and light and is suitable for use in cosmetics. The niacinamide is known as water-soluble vitamin B3 or vitamin PP. In foods, it can be present as the vitamin itself or as tryptophan, where 60 mg of tryptophan is converted to 1 mg of niacinamide.

The niacinamide promotes skin health and mental health, energy regeneration of cells, and helps keep the digestive system healthy. Lack of absorption of the niacinamide can lead to a vitamin deficiency called pellagra or erythema. Pellagra is a pigmented rash of sun-exposed skin, inflammation of the mucous membranes of the digestive tract, skin cancer, digestive tract disorders such as vomiting, constipation or diarrhea, bright red tongue, depression, apathy, headache, and fatigue. , and neurological disorders such as memory loss.

The niacinamide component increases moisture retention, softens the skin, and forms a sense of volume in the skin. Fatty acids and ceramides that form the skin barrier on the skin do not perform their functions properly when the skin loses moisture. At this time, the niacinamide can help to form and strengthen the skin barrier by maintaining skin moisture. In addition, it is effective in skin whitening by helping to uniformize the overall skin tone by softening or suppressing melanin pigmentation.

The adenosine is a white needle crystal, has a melting point of 235 ° C, is very stable to heat, light, and sunlight, and is widely used as a wrinkle functional cosmetic raw material, but has low solubility, so it is difficult to sufficiently show its efficacy in cosmetic formulations. It is 0.7% soluble in water at room temperature, but its solubility is very low at low temperatures, and it precipitates over time, and it is hardly soluble in alcohol, so it is difficult to find cosmetics containing more than 1.0%.

The adenosine is a nucleoside involved in signal transduction outside the cell, and serves to promote DNA synthesis and increase protein synthesis in skin fibroblasts. In addition, according to a recent study, adenosine is effective in preventing hair loss and growing hair. There is a report that adenosine is rather effective compared to minoxidil (Minoxidil) as a representative ingredient for preventing hair loss and hair growth. Minoxidil has hair loss and hair growth effects, but there may be side effects such as hormonal changes and blood pressure fluctuations, but adenosine has no known side effects.

The vulgaris mugwort oil (Artemisia Vulgaris Oil) can be prepared from vulgaris mugwort, a perennial herbaceous plant belonging to the Asteraceae family. , it can help prevent oxidative damage to the skin by being involved in removing the generated active oxygen.

Components contained in the Bulgarian mugwort include terpene compounds such as artemisinin and artemisinic acid, and it is reported that the components contained in the Bulgarian mugwort differ depending on the collection species and cultivation method. However, since the vulgaris mugwort warms the blood and meridians, it shows a hemostatic response to uterine bleeding, bleeding during pregnancy, vomiting blood, nosebleeds, and hemoptysis caused by weak and cold uterus and lower abdomen. In addition, it is used for symptoms such as weakness and cold, cold feeling and pain in the abdomen, menstrual irregularity, dysmenorrhea, and pruritus, and is also effective for eczema and skin itching. It is known that pharmacological actions include hemostasis, antibacterial action, bronchial smooth muscle relaxation, antitussive expectoration, sleep action, uterine excitation induction, and hypersensitivity shock protection.

On the other hand, the cosmetic composition having UV protection and whitening and wrinkle improvement effects according to an embodiment of the technical idea of the present invention includes complex nanoliposomes, macadamia oil, and moringa seed oil in addition to the zinc oxide, niacinamide, adenosine, and vulgaris mugwort oil. , black pine needle fermented extract, ethanol, 1,2-hexanediol, centella asiatica extract, allantoin, ethylhexyl glycerin, glyceryl caprylate and purified water may be further included.

In addition, the cosmetic composition having UV protection and whitening and wrinkle improvement effects according to an embodiment of the technical idea of the present invention contains 1 to 5% by weight of zinc oxide, 2 to 5% by weight of niacinamide, 0.01 to 0.1% by weight of adenosine, and vulgaris mugwort. 2 to 6% by weight of oil, 1 to 3% by weight of complex nanoliposome, 0.5 to 2.5% by weight of macadamia oil, 0.5 to 2.5% by weight of moringa seed oil, 0.3 to 0.7% by weight of fermented extract of black pine needles, 8 to 12% by weight of ethanol , 1 to 3% by weight of 1,2-hexanediol, 0.1 to 1% by weight of centella asiatica extract, 0.15 to 0.35% by weight of allantoin, 1 to 3% by weight of ethylhexylglycerin, 0.1 to 1% by weight of glyceryl caprylate and the remaining amount of purified water can include

The complex nanoliposome may be prepared by including salmon testis protein extract and camellia oil. The salmon testis protein extract is an extract derived from salmon testicular protein and contains a large amount of nucleic acid and protein. The nucleic acids of the salmon testis protein extract include both DNA and RNA, and the protein is a basic protein containing a large amount of basic amino acids such as arginine. It has been reported to have microbial effects and electron donating ability.

In particular, nucleic acids and protamine, which are abundantly distributed in salmon testis, are essential proteins for our body and are important components of antioxidant enzymes that protect cells from free radicals generated during normal oxygen metabolism.

For example, salmon testicular protein extract contains a large amount of nucleic acids, and shows excellent effects on skin improvement or immunity improvement. Specifically, adenylic acid (AMP), thymidylic acid (TMP), and guanylic acid (GMP) , Cytidine monophosphate (CMP) component promotes differentiation and proliferation of cells and induces suicide of cancer cells. In addition, nucleic acids activate NK cells, expel malignant cells and senescent cells, and promote the production of normal cells.

The salmon testis protein extract is a known substance, and the configuration of preparing the salmon testis protein extract from the salmon testis protein is a known technique, so a detailed description thereof will be omitted for convenience of description and clarity of the technical idea of the present invention. do it with

In addition, the camellia oil can be extracted from the seeds of camellia. Camellia oil is known to contain a lot of unsaturated fatty acids such as omega-3 fatty acids and omega-6 fatty acids, and especially contains a lot of oleic acid. may have been The oleic acid helps to remove active oxygen, and can act as an effect of preventing dehydration of moisture in the skin and forming a protective film.

In addition, the camellia oil has a very excellent moisturizing effect, is effective in treating atopy, and has an excellent conditioning effect, so it has been used to care for hair and skin since ancient times. It has the effect of suppressing the oxidation of sebum and is widely used for aging skin and dry skin.

The composite nano-liposome may be a composite nano-liposome prepared by the following method.

That is, the composite nanoliposome containing the salmon testis protein extract and camellia oil as active ingredients, (A) 1 to 10 parts by weight of the peptide mixture, ethyl 8 to 12 parts by weight of hexylglycerin, 1 to 10 parts by weight of salmon testis protein extract, 1 to 10 parts by weight of behenyl alcohol, 10 to 20 parts by weight of 1,2-butylene glycol, panthenyltriacetate (Panthenyl preparing an aqueous phase by mixing 3 to 7 parts by weight of triacetate and 0.1 to 1 part by weight of isethionate with 40 to 60 parts by weight of purified water at a temperature of 60 to 70 ° C;

(B) 10 to 20 parts by weight of phospholipids, 5 to 15 parts by weight of dimethicone, 5 to 10 parts by weight of camellia oil, 0.5 to 1.5 parts by weight of fatty acids, Preparing an oil phase by mixing 0.5 to 3.0 parts by weight of cholesterol, 1 to 10 parts by weight of ceramide complex, 1 to 5 parts by weight of lecithin (Hydrogenated Lecithin) and 0.1 to 1 part by weight of sodium methyl cocoyl taurate at a temperature of 60 to 70 ° C. ;

(C) After heating the water phase prepared in step (A) at a temperature of 50 to 60 ° C, adding the oil phase prepared in step (B) and using a homomixer at 4,000 to 5,000 rpm for 20 to 40 minutes After emulsification, cooling to room temperature and preparing a water-in-oil type (W / O type) complex nanoliposome through high-pressure emulsification using a microfluidizer to permeate 3 to 5 times under 900 to 1,100 Bar conditions; and

(D) The water-in-oil type (W/O type) complex nanoliposome prepared in step (C) is mixed again with the water phase part from which the active ingredient is removed from the water phase part of step (A) and the oil phase part of step (B). and emulsifying using a homomixer at 50 to 60 ° C. at 4,000 to 5,000 rpm for 10 to 30 minutes to prepare a multi-emulsion type (W / O / W type) complex nanoliposome emulsion.

In the step (D), the water-in-oil (W/O type) complex nanoliposome may be included in an amount of 10 to 20% by weight based on the total weight of the complex nanoliposome emulsion.

The peptide mixture refers to a mixture of 2 to less than 30 amino acids, which are structural units constituting a protein, and includes, for example, human oligopeptide-1, RH-polypeptide-1, acetylhexapeptide-8, copper At least one selected from the group consisting of tripeptide-1, palmitoyl tripeptide-5, and caffeoyl tripeptide-1 may be used.

The salmon testis protein extract is an extract derived from salmon testis protein and contains a large amount of nucleic acid and protein.

The phospholipid is an ester compound of phosphoric acid, and includes natural phospholipids and synthetic phospholipids, and both saturated and unsaturated types can be used, preferably having a fatty acid chain having 12 to 30 carbon atoms. Contains phospholipids. For example, the phospholipids include phosphatidylcholine (PC; natural or synthetic lecithin), dipalmitoylphosphatidylcholine, phosphatidic acid (PA), lysophosphatidylcholine (LPC), phosphatidylserin (PS) At least one selected from the group consisting of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylinositol (PI) may be used.

The camellia oil may be oil extracted from camellia seeds.

The fatty acid is a saturated fatty acid having 10 to 24 carbon atoms or an unsaturated fatty acid having a carbon chain that is one or more unsaturated bonds. For example, the fatty acid is linoleic acid, oleic acid, lauric acid, palmitic acid, capric acid, hepta At least one selected from the group consisting of decanoic acid, pentadecanoic acid, arachidic acid, arachidonic acid and erucic acid may be used.

The ceramide refers to a compound composed of sphingosine and fatty acids, and is also referred to as N-acylsphingosine. The ceramide is a main component of the stratum corneum of the epidermal layer of the skin. For example, the ceramide may include at least one selected from the group consisting of ceramide EOP, ceramide NS, ceramide NP, ceramide AS, and ceramide AP. The ceramide can fill between phospholipids in the particle walls of one aspect of the nanoemulsion and make the particle walls of the nanoemulsion hard.

The macadamia oil can be prepared by extracting oil from macadamia nuts. The macadamia fruit is composed of 75% of natural edible oil, 9% of protein, 9% of carbohydrates and 2% of dietary fiber, and the rest is human nutrition It contains calcium, phosphorus, sodium, vitamins E, B1, B2, niacin, iron, etc., which are essential elements in the body, and contains selenium, an essential trace element in the body known to be effective in delaying the onset of AIDS and promoting growth in children, in addition to anticancer. has been

The vitamins and unsaturated fatty acids contained in the macadamia add moisture to dry skin, help blood circulation, and increase the vitality of skin cells. In addition, it contains unsaturated fatty acids, proteins, and minerals to protect the skin from active oxygen and has an anti-aging effect, helping to maintain smooth and healthy skin.

The Moringa seed oil can be prepared by extracting oil from Moringa Seed. The Moringa Seed is a seed of the Moringa tree, and the Moringa tree belongs to the Moringaceae Family, As a plant of the Moringa genus, its scientific name is Moringa Oleifera (another name Moringa Pterygosperma), and it is also called Murunggai in Tamil, where the scientific name was derived. Moringa is a leguminous plant, a tropical tree whose leaves and fruits as well as the whole tree are edible, and contains more than 90 nutrients such as a large amount of amino acids, minerals, and vitamins.

In particular, the water-soluble protein contained in Moringa seeds plays a role in purifying pollutants in water. When these water-soluble proteins are applied to the skin, they show a detoxifying effect that cleanses the skin by purifying pollutants on the skin. In addition, since it contains a large amount of carotenoid components such as beta-carotene and rutin, and vitamins and minerals, it can brighten dull skin and realize a whitening effect.

The black pine needle fermented extract can be prepared using pine needles. The pine needles are the leaves of pine trees (Pinus densiflora Siebold et Zuccarini), and the leaves of trees with a long history in Korea, China, and Japan in Northeast Asia. and new order Fresh pine needles contain a large amount of ascorbic acid, vitamins A, B, K, bitter substances, flavonoids, anthocyanins, 7 to 12% of resin (rosin), 5% of tannins, carbohydrates, essential oil, etc. contains this.

In addition, the whole pine tree contains components that discharge wastes from the body such as alcohol and esters and promote metabolism, phenolic compounds, quinine, terpentine, vitamins A, C, and chlorotyl as main components, as well as glycoginin and apietic acid. It is also rich in iron, and 24 kinds of amino acids contained in the leaves of red pine, and 19 kinds of amino acids composed of proteins were also confirmed.

The pine needles are a raw material and source of vitamin C, and the pine needles have a much darker green color, so rich carotene can be consumed. In the case of pine needles, the pharmacological value can refer to the general effects of terpenes, phenolic compounds, and tannins. In addition, high blood pressure, stroke, diabetes, dementia and aging prevention, anticancer effect, liver and stomach, constipation and anemia, chronic alcoholism and hangover relief, nicotine removal, skin care and constitution improvement, diet men's stamina, various stresses, excessive smoking and drinking When your head is heavy and your eyes are blurry, it is especially good for students or examinees who are studying by clearing their heads.

The black pine leaf fermentation extract prepared by the following method may be used.

In order to prepare the black pine needle fermented extract, first, pine needles may be prepared and then washed with purified water.

In the above step, dust or foreign substances attached to the pine needles can be removed by preparing the pine needles and then washing them in purified water at a temperature of 20 to 30 ° C. The prepared pine needles may be pine needles containing pine needle sprouts.

Next, the washed pine needles may be immersed in rice water.

In the above step, the rosin remaining in the pine needles can be removed by immersing the washed pine needles in rice water, and 400 to 600 parts by weight of rice water is mixed and immersed in a weight ratio of 400 to 600 parts by weight with respect to 100 parts by weight of the total washed pine needles. After that, it may proceed by maintaining for 2 to 4 days at a temperature of 8 to 12 ° C.

Next, the pine needles immersed in the rice water may be separated and then dried.

In the above step, the pine needles immersed in the rice water may be separated and then dried at a temperature of 20 to 30 ° C. for 20 to 40 hours.

Subsequently, black pine needles can be prepared by steaming the dried pine needles.

In the above step, the black pine needles may be prepared by steaming the dried pine needles at a temperature of 75 to 80 ° C. for 5 to 7 days.

Next, after mixing the steamed black pine needles and the yeast culture solution, a fermented black pine needle solution may be prepared by fermentation.

In the above step, 10 to 20 parts by weight of the yeast culture solution is mixed in a weight ratio with respect to 100 parts by weight of the steamed black pine needles, and then fermented at a temperature of 43 to 47 ° C. for 5 to 7 days to prepare a fermented black pine needle solution.

In addition, the yeast culture solution may be a Moringa extract yeast ( Saccharomyces spp. ) fermentation broth, the Moringa extract yeast ( Saccharomyces spp. ) fermentation broth, Moringa ( Moringa oleifera L. ) leaves at 30 to 40 ℃ Dried Moringa leaves obtained by drying for 5 to 10 days at temperature are immersed in 40 times the volume of distilled water, extracted at 85 to 87 ° C and 1.5 Pa, concentrated by 50%, and then the residue is removed to prepare a Moringa extract. Yeast ( Saccharomyces spp. ) was inoculated into the Ringa extract at a concentration of 5 × 1.0 ⁶ ~ 10 × 1.0 ⁶ CFU / mL, incubated at 38 to 42 ° C for 30 hours, and centrifuged at 10,000 to 15,000 rpm for 10 to 15 minutes Moringa extract yeast fermentation broth prepared by taking the supernatant and sterilizing it at 120 to 140 ° C. for 30 to 50 minutes may be used.

Next, a black pine needle fermented extract can be prepared by extracting the black pine needle fermented liquid.

In the above step, the extraction of the black pine needle fermented liquid may be performed using various extraction methods such as hot water extraction, organic solvent extraction, ultrasonic extraction, and supercritical extraction. For clarity of the technical idea of , a detailed description thereof will be omitted.

Hereinafter, with reference to the accompanying drawings, an example of a cosmetic composition having UV protection and whitening and wrinkle improvement effects according to an embodiment of the technical idea of the present invention will be described in more detail.

<Example 1>

Zinc oxide 3% by weight, niacinamide 3.5% by weight, adenosine 0.04% by weight, vulgaris mugwort oil 4% by weight, complex nanoliposome 2% by weight, macadamia oil 1.5% by weight, moringa seed oil 1.5% by weight, black pine needle fermented extract 0.5% by weight % by weight, 10% by weight of ethanol, 2% by weight of 1,2-hexanediol, 0.5% by weight of centella asiatica extract, 0.25% by weight of allantoin, 2% by weight of ethylhexylglycerin, 0.5% by weight of glyceryl caprylate and the remaining amount of purified water were mixed. A cosmetic composition was prepared.

At this time, the complex nano-liposome is (A) 5 parts by weight of the peptide mixture, 10 parts by weight of ethylhexyl glycerin, 5 parts by weight of salmon testis protein extract, 5 parts by weight of behenyl alcohol, based on the total weight of the water phase and the oil phase Preparing an aqueous phase by mixing 15 parts by weight of 1,2-butylene glycol, 5 parts by weight of Panthenyl Triacetate and 0.5 parts by weight of isethionate with 50 parts by weight of purified water at 65 ° C; (B) 15 parts by weight of phospholipid, 10 parts by weight of dimethicone, 7 parts by weight of camellia oil, 1 part by weight of fatty acid, 1.5 parts by weight of cholesterol, 5 parts by weight of ceramide complex, lecithin (Hydrogenated Lecithin) ) preparing an oil phase by mixing 3 parts by weight and 0.5 parts by weight of sodium methyl cocoyl taurate at a temperature of 65 ° C; (C) After warming the water phase prepared in step (A) at a temperature of 55 ° C, adding the oily phase prepared in step (B) and emulsifying using a homomixer at 4,500 rpm for 30 minutes, then returning to room temperature Preparing a water-in-oil type (W / O type) complex nanoliposome through high-pressure emulsification by cooling and permeating 4 times under 1,000 Bar conditions using a microfluidizer; And (D) the water-in-oil type (W / O type) complex nanoliposome prepared in step (C) is mixed with the water phase part in which the active ingredient is removed from the water phase part in step (A), and the oil phase part in step (B). It was prepared by mixing and emulsifying using a homomixer at 55 ° C. at 4,500 rpm for 20 minutes to prepare a multi-emulsion type (W / O / W type) complex nanoliposome emulsion. The water type (W/O type) complex nanoliposome was included in an amount of 15% by weight based on the total weight of the complex nanoliposome emulsion.

<Example 2>

A cosmetic composition was prepared using the same composition as in Example 1. In Example 2, 4% by weight of zinc oxide, 2.5% by weight of niacinamide, 0.02% by weight of adenosine, 5% by weight of vulgaris mugwort oil, 2.5% by weight of complex nanoliposomes, Macadamia oil 1% by weight, moringa seed oil 2% by weight, black pine needle fermented extract 0.4% by weight, ethanol 11% by weight, 1,2-hexanediol 1.5% by weight, centella asiatica extract 0.9% by weight, allantoin 0.2% by weight, ethylhexyl A cosmetic composition was prepared by mixing 1.5% by weight of glycerin, 0.2% by weight of glyceryl caprylate, and the remaining amount of purified water.

<Example 3>

A cosmetic composition was prepared using the same composition as in Example 1. In Example 3, 2% by weight of zinc oxide, 4.5% by weight of niacinamide, 0.8% by weight of adenosine, 3% by weight of vulgaris mugwort oil, 1.5% by weight of complex nanoliposomes, Macadamia oil 2% by weight, moringa seed oil 1% by weight, black pine needle fermented extract 0.6% by weight, ethanol 9% by weight, 1,2-hexanediol 2.5% by weight, centella asiatica extract 0.3% by weight, allantoin 0.3% by weight, ethylhexyl A cosmetic composition was prepared by mixing 2.5% by weight of glycerin, 0.8% by weight of glyceryl caprylate and the remaining amount of purified water.

<Comparative Example 1>

4% by weight of bulgaris mugwort oil, 1.5% by weight of macadamia oil, 1.5% by weight of moringa seed oil, 0.5% by weight of fermented extract of black pine needles, 10% by weight of ethanol, 2% by weight of 1,2-hexanediol, 0.5% by weight of centella asiatica extract, A cosmetic composition was prepared by mixing 0.25% by weight of allantoin, 2% by weight of ethylhexyl glycerin, 0.5% by weight of glyceryl caprylate, and the remaining amount of purified water.

<Comparative Example 2>

Zinc oxide 3 wt%, complex nanoliposome 2 wt%, macadamia oil 1.5 wt%, moringa seed oil 1.5 wt%, ethanol 10 wt%, 1,2-hexanediol 2 wt%, centella asiatica extract 0.5 wt%, allantoin 0.25 wt% A cosmetic composition was prepared by mixing 2 wt% of ethylhexyl glycerin, 0.5 wt% of glyceryl caprylate, and the remaining amount of purified water.

1. Evaluation of skin brightness improvement

The cosmetic compositions according to Examples 1, 2 and 3 and the cosmetic compositions according to Comparative Examples 1 and 2 were evaluated for their skin brightness improvement effects.

1 is a graph showing the skin brightness improvement effect of the cosmetic compositions according to Examples 1, 2 and 3 and the cosmetic compositions according to Comparative Examples 1 and 2.

The skin brightness improvement effect was measured by using Chromameter CR-400 for 35 selected women between the ages of 20 and 40, and then measuring the skin brightness before use, and then using the cosmetic composition according to Examples 1, 2, and 3 , Skin brightness was remeasured using the cosmetic compositions according to Comparative Examples 1 and 2 individually.

The measurement results are shown in [Table 1] and FIG. 1 below, and the measured values are shown as the average value of three times excluding the maximum and minimum values of L*.

[Skin brightness (L* Arbitrary unit)] division Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 before use 60.12 60.16 60.21 61.35 61.27 after use 67.84 67.58 67.81 64.97 64.01 Increase (%) 12.84% 12.3% 12.6% 5.9% 4.47%

Referring to [Table 1] and FIG. 1, it can be confirmed that the skin brightness of the cosmetic compositions according to Examples 1, 2, and 3 was significantly improved compared to the cosmetic compositions according to Comparative Examples 1 and 2.

2. Evaluation of skin wrinkle improvement effect

Skin wrinkle improvement effects of the cosmetic compositions according to Examples 1, 2 and 3 and the cosmetic compositions according to Comparative Examples 1 and 2 were evaluated.

Figure 2 is a graph showing the skin wrinkle improvement effect of the cosmetic compositions according to Examples 1, 2, and 3 and the cosmetic compositions according to Comparative Examples 1 to 4.

The skin wrinkle improvement effect was measured for skin wrinkle improvement in 50 women aged 30 years or older.

The cosmetic compositions according to Examples 1, 2, and 3 and the cosmetic compositions according to Comparative Examples 1 and 2 were applied around the eyes twice a day (morning and evening) for 10 weeks, using an ANTERA 3D skin wrinkle improvement measuring instrument The skin wrinkle improvement effect of the subject was measured.

The test results were described as A1 (A1 (10 weeks) - A1 (0 weeks)) value of the measuring instrument. The A1 value represents the property of skin wrinkle viscoelasticity, and the results are shown in [Table 2] and FIG. 2 below.

division Skin wrinkle improvement effect Example 1 1.02 Example 2 0.98 Example 3 0.94 Comparative Example 1 0.67 Comparative Example 2 0.63

Referring to [Table 2] and FIG. 2, it can be confirmed that the cosmetic compositions according to Examples 1, 2 and 3 have significantly better skin wrinkle improvement effects than the cosmetic compositions according to Comparative Examples 1 and 2.

3. Measure the concealment rate

In order to find out the UV blocking effect of the cosmetic composition, the part that must be considered is the cloudiness. In order to confirm the cloudiness, the coverage of the cosmetic compositions according to Examples 1, 2 and 3 and the cosmetic compositions according to Comparative Examples 1 and 2 were measured and described in [Table 3] below. The concealment ratio was calculated using Equation 1 below, and the closer to 1, the higher the concealment ratio (= opacity, covering power).

The concealment rate was measured by applying the same amount of cosmetics according to Examples 1, 2 and 3 and cosmetics according to Comparative Examples 1 and 2 with an applicator for forming a coating film on black and white paper to form a coating film, drying for 1 hour, and then using a colorimeter (Coloremeter NR-60CP). ) was measured.

[Equation 1]

division Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 concealment rate 1.51 1.64 1.70 3.64 4.01

Referring to [Table 3], as a result of measuring the coverage, it can be confirmed that the coverage of the cosmetic composition according to Examples 1, 2 and 3 is superior to the cosmetic composition according to Comparative Examples 1 and 2.

4. Experiments on whitening improvement

Melanin pigment is the main cause of skin aging phenomena such as freckles and age spots, and tyrosinase is a major enzyme that generates melanin pigment. The enzyme activity inhibitory ability of the test substance was measured by comparing the amount of DOPA (dihydroxyphenylalanine) or dopachrome produced by Naze.

The test method used tyrosine as a substrate, put it into the enzyme reaction solution, reacted for a certain time, and measured the absorbance at 475 nm, which is the absorption wavelength of the reaction product, dopachrome.

500 μl of L-tyrosine (0.3 mg/ml) and 450 μl of the cosmetics according to Example 1 and Comparative Example 1 were added to 500 μl of 0.1M Potassium phosphate buffer solution (pH 6.8), followed by reaction on a 37°C water bath for 10 minutes. The reaction is quenched by quickly placing in the refrigerator for 5 minutes. After adding 50 μl of tyrosinase (1,250 units/ml) and incubating for 10 minutes, absorbance was measured at 475 nm. As a control, 450 μl of the sample solvent is added instead of the test solution. For the color correction blank, 50 μl of potassium phosphate buffer solution (pH 6.8, 0.1 M) was added instead of tyrosinase, and a sample of the same concentration as the experimental group was added. Tyrosinase inhibitory activity was calculated using the following equation and shown in [Table 4].

% inhibition = [(A-B)/A] x 100

A: Absorbance after reaction of a reaction solution (control) that does not contain a test sample (inhibitor)

B: Absorbance after reaction of the reaction solution containing the test sample.

division Tyrosinase inhibitory activity rate (%) Example 1 36.37 Comparative Example 1 15.11 Positive control (Arbutin) 41.27

Referring to [Table 4], it was confirmed that the cosmetic composition according to Example 1 showed a tyrosinase inhibition rate of 36.37%, whereas the cosmetic composition according to Comparative Example 1 had a tyrosinase inhibition rate of 15.11%.

In addition, the cosmetic composition according to Example 1 showed a slightly smaller inhibition rate than the control group, Arbutin. Therefore, the cosmetic composition according to Example 1 can be said to be effective in inhibiting tyrosinase.

5. Measurement of moisturizing effect

A capacitance measurement method using a Corneometer CM820 (Courage, Germany) was used, and the moisturizing effect was compared and measured using the cosmetic composition according to Example 1 and the cosmetic composition according to Comparative Example 1 as samples.

15 women between the ages of 20 and 50 were allowed to adapt to the inner surface of the lower arm in a constant temperature and humidity room for 20 minutes just before the start of the test, and then the skin moisture was kept constant. Then, after measuring the initial moisture content of each sample application site using Corneometa C820, the cosmetic composition according to Example 1 and the cosmetic composition according to Comparative Example 1 were applied.

After 20 minutes of application, the moisture change rate was first measured, and the moisture change rate was measured again after 2 hours. Each measurement site was repeatedly measured three times, and the skin moisture change rate was calculated according to the following [Equation 2] as the average value, and it is shown in [Table 5].

[Equation 2]

Moisture change rate (%) = (Tdi-Tdo)-(Ntdi-NTdo) / ((Ntdi-Ntdo) + Tdo) * 100

Tdi: nth measured value of the sample application area

Tdo: measured value before sample application

Ntdi: nth measured value of the area where the sample is not applied

NTdo: measured value before application of the area where the sample was not applied

division Example 1 Comparative Example 1 20 minutes 33.23 8.02 120 minutes 22.78 2.17

Referring to [Table 5], it was confirmed that the cosmetic composition according to Example 1 was superior to the cosmetic composition according to Comparative Example 1 in moisturizing effect not only in the initial stage but also over time.

Although the preferred embodiment of the present invention has been described above, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. You will be able to. Therefore, one embodiment described above should be understood as illustrative in all respects and not limiting.

Claims (6)

Contains zinc oxide, niacinamide, adenosine and vulgaris mugwort oil as active ingredients,
1 to 5% by weight of the zinc oxide, 2 to 5% by weight of niacinamide, 0.01 to 0.1% by weight of adenosine, and 2 to 6% by weight of vulgaris mugwort oil,
In addition to the above zinc oxide, niacinamide, adenosine, and vulgaris mugwort oil, complex nanoliposomes, macadamia oil, moringa seed oil, black pine needle fermented extract, ethanol, 1,2-hexanediol, centella asiatica extract, allantoin, ethylhexylglycerin, glycerin A cosmetic composition having UV protection and whitening and anti-wrinkle effects, further comprising lycaprylate and purified water.
delete delete According to claim 1,
1 to 5% by weight of the zinc oxide, 2 to 5% by weight of niacinamide, 0.01 to 0.1% by weight of adenosine, 2 to 6% by weight of vulgaris mugwort oil, 1 to 3% by weight of complex nanoliposomes, 0.5 to 2.5% by weight of macadamia oil, Moringa seed oil 0.5 to 2.5% by weight, black pine needle fermented extract 0.3 to 0.7% by weight, ethanol 8 to 12% by weight, 1,2-hexanediol 1 to 3% by weight, centella asiatica extract 0.1 to 1% by weight, allantoin 0.15 to 12% by weight 0.35% by weight, 1 to 3% by weight of ethylhexyl glycerin, 0.1 to 1% by weight of glyceryl caprylate and the remaining amount of purified water A cosmetic composition with UV protection and whitening and wrinkle improvement effects.
According to claim 4,
The composite nano-liposome is a cosmetic composition with UV protection and whitening and anti-wrinkle effects, characterized in that prepared by including salmon testis protein extract and camellia oil.
According to claim 5,
The zinc oxide 3% by weight, niacinamide 3.5% by weight, adenosine 0.04% by weight, vulgaris mugwort oil 4% by weight, complex nanoliposome 2% by weight, macadamia oil 1.5% by weight, moringa seed oil 1.5% by weight, black pine needle fermented extract 0.5% by weight, 10% by weight of ethanol, 2% by weight of 1,2-hexanediol, 0.5% by weight of centella asiatica extract, 0.25% by weight of allantoin, 2% by weight of ethylhexyl glycerin, 0.5% by weight of glyceryl caprylate and the remaining amount of purified water A cosmetic composition having UV protection and whitening and wrinkle improvement effects, characterized in that.

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