KR102453269B1 - Cosmetic composition containing starfish extract and hydrolyzed collagen as active ingredients to improve skin wrinkles, promote collagen synthesis, and moisturize - Google Patents

Abstract

The present invention provides a cosmetic composition containing a starfish extract and hydrolyzed collagen as active ingredients. The cosmetic composition according to the present invention has a skin wrinkle improvement effect, a collagen synthesis promoting effect, and a moisturizing effect.

Description

Cosmetic composition containing starfish extract and hydrolyzed collagen as active ingredients to improve skin wrinkles, promote collagen synthesis , and moisturize}

The present invention relates to a cosmetic composition containing a starfish extract and hydrolyzed collagen as active ingredients, which has a skin wrinkle improvement effect, a collagen synthesis promoting effect, and a moisturizing effect.

Human skin undergoes various physical and chemical changes during the aging process, and the causes are largely divided into intrinsic aging and photo-aging, and studies on this have been actively conducted. Free radicals are activated and caused by UV rays, stress, disease states, environmental factors, wounds, and aging. and accelerate aging.

That is, lipids, proteins, polysaccharides, nucleic acids, etc., which are major constituents of the skin, are oxidized, and skin cells and tissues are destroyed, resulting in skin aging. In particular, the oxidation of proteins cuts the connective tissues of the skin, such as collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, causing severe hyperinflammatory reactions and disruption of skin elasticity. And if it gets worse, it leads to a situation of mutation, cancer induction, and decreased immune function due to DNA mutation. Therefore, it protects the cell membrane by removing free radicals generated during the body's metabolic process, UV irradiation, and free radicals mediated by inflammatory reactions. You can recover and maintain healthy skin.

In aging, not only free radicals but also enzymes called MMPs are involved. In vivo, the synthesis and degradation of extracellular matrix such as collagen is properly regulated, but as aging progresses, its synthesis decreases, and matrix metalloproteinase, an enzyme that breaks down collagen ( MMP) expression is promoted, the elasticity of the skin is reduced and wrinkles are formed. In addition, these decomposing enzymes are also activated by UV irradiation.

Therefore, studies on substances with anti-aging and anti-wrinkle properties have been actively conducted, but existing chemical substances are limited in their use due to various problems such as toxicity, low activity, limitations in use, and side effects due to overdose. Research on extracting active ingredients from natural plants is active as part of the development of less likely materials. However, even in the case of natural products, in terms of safety, stability, discoloration, etc., there are many problems when used in cosmetics or pharmaceuticals at an effective concentration or more, and it does not produce satisfactory effects.

Patent Document 1: Korean Patent Publication No. 10-2009-0025422

The present invention has been devised to solve the above problems, and an object of the present invention is to provide a cosmetic composition having a skin wrinkle improvement effect, a collagen synthesis promoting effect, and a moisturizing effect.

The problems of the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the above object, the present invention

It provides a cosmetic composition containing a starfish extract and hydrolyzed collagen as active ingredients.

In addition, the cosmetic composition comprises: 1-5 parts by weight of the first extract comprising a starfish extract, a sea cucumber extract and a sea urchin extract in a weight ratio of 4:3:3; The second extract 1-5 comprising pine needle extract, lotus leaf extract, lotus seed extract, honeysuckle flower extract, propolis extract, centella asiatica extract and gilkyung extract in a weight ratio of 2.5:2:1.5:1:1:1:1 parts by weight; 0.1-1 parts by weight of hydrolyzed collagen; 4-8 parts by weight of hyaluronic acid; 3-7 parts by weight of butylene glycol; 1-5 parts by weight of glycerin; 1-5 parts by weight of shea butter; 1-5 parts by weight of propanediol; 0.1-1 parts by weight of tris(hydroxymethyl)aminomethane; Cholesterol 0.1-1 parts by weight; 0.1-1 parts by weight of jojoba oil; 0.1-1 parts by weight of rosemary leaf oil; 0.05-0.5 parts by weight of hydrogenated poly(C6-14 olefin); 0.05-0.5 parts by weight of carbomer; 0.1-1 parts by weight of polysorbate 80; 2-6 parts by weight of dicapryl carbonate; 0.5-4 parts by weight of 1,2-hexanediol; 3-7 parts by weight of dipropylene glycol; and 58-62 parts by weight of purified water.

Also, the present invention

Washing the starfish, cut into appropriate sizes, freeze-drying, and pulverizing to prepare a starfish powder; Preparing an extract by mixing the starfish powder, Tris buffer solution, and a protein hydrolysis inhibitor and stirring at a temperature of 3-7° C. for 90-150 minutes; and centrifuging the extract to separate the supernatant and filtering it with a filter paper; preparing a starfish extract comprising;

Obtaining and washing sea cucumber intestines from sea cucumbers, cut into appropriate sizes, freeze-drying, and pulverizing to prepare sea cucumber intestines powder; extracting a solution containing the active ingredient by mixing the sea cucumber intestine powder with ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure; preparing a sea cucumber extract comprising;

Obtaining sea urchin roe from sea urchin, washing, cutting to an appropriate size, freeze-drying, and pulverizing to prepare sea urchin powder; extracting a solution containing an active ingredient by mixing the sea urchin powder with ethanol; and concentrating and lyophilizing the extracted solution under reduced pressure; preparing a sea urchin extract comprising;

Preparing a first mixture comprising pine needles, lotus leaves and lotus seeds in a weight ratio of 2.5:2:1.5 by washing pine needles, lotus leaves and lotus seeds, cut into appropriate sizes, freeze-dried, and pulverized; The first mixture, a mixed solvent of ethanol and acetone mixed in a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMIm][TFSI], 1 Extracting a solution containing an active ingredient by mixing -Ethyl-3-methylimidazolium bis (trifluoromethylsulfylimide); and concentrating and freeze-drying the extracted solution under reduced pressure; pine needle extract, lotus leaf extract and lotus seed extract, including Preparing a first natural extract comprising;

Honeysuckle flower, propolis, centella asiatica and gilts are washed, cut into appropriate sizes, freeze-dried, and pulverized to give honeysuckle flower powder, propolis powder, centella asiatica powder and gilkyong powder in a weight ratio of 1:1:1:1. Preparing a second mixture comprising; mixing the second mixture and water, performing agitation, and performing primary hot water extraction at a temperature of 115-125° C. for 6-12 hours; After performing the first hot water extraction, filtration with filter paper, and then freeze-drying the filtrate to prepare a first mixed extract; After performing the primary hot water extraction, filtering with filter paper, and then mixing the remaining solid with water again, performing secondary hot water extraction at a temperature of 85-95° C. for 20-28 hours; After the secondary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a second mixed extract; and mixing the first mixed extract and the second mixed extract; preparing a second natural extract including honeysuckle flower extract, propolis extract, centella asiatica extract and gilgyeong extract;

preparing a first extract by mixing the starfish extract, sea cucumber extract and sea urchin extract in a weight ratio of 4:3:3;

preparing a second extract by mixing the first natural extract and the second natural extract in a weight ratio of 3:2; and

1-5 parts by weight of the first extract; 1-5 parts by weight of the second extract; 0.1-1 parts by weight of hydrolyzed collagen; 4-8 parts by weight of hyaluronic acid; 3-7 parts by weight of butylene glycol; 1-5 parts by weight of glycerin; 1-5 parts by weight of shea butter; 1-5 parts by weight of propanediol; 0.1-1 parts by weight of tris(hydroxymethyl)aminomethane; Cholesterol 0.1-1 parts by weight; 0.1-1 parts by weight of jojoba oil; 0.1-1 parts by weight of rosemary leaf oil; 0.05-0.5 parts by weight of hydrogenated poly(C6-14 olefin); 0.05-0.5 parts by weight of carbomer; 0.1-1 parts by weight of polysorbate 80; 2-6 parts by weight of dicapryl carbonate; 0.5-4 parts by weight of 1,2-hexanediol; 3-7 parts by weight of dipropylene glycol; And 58-62 parts by weight of purified water; mixing; provides a method for preparing a cosmetic composition comprising a.

The cosmetic composition according to the present invention has a skin wrinkle improvement effect, a collagen synthesis promoting effect, and a moisturizing effect.

1 is a flowchart showing a method for preparing a cosmetic composition provided in one aspect according to the present invention.

Hereinafter, various embodiments will be described in more detail with reference to the accompanying drawings. The embodiments described herein may be variously modified. Certain embodiments may be depicted in the drawings and described in detail in the detailed description. However, specific embodiments disclosed in the accompanying drawings are only provided to facilitate understanding of various embodiments. Therefore, the technical spirit is not limited by the specific embodiments disclosed in the accompanying drawings, and it should be understood to include all equivalents or substitutes included in the spirit and scope of the invention.

Terms including ordinal numbers such as first, second, first, second, etc. may be used to describe various elements, but these elements are not limited by the above-described terms. The above terminology is used only for the purpose of distinguishing one component from another component.

In this specification, terms such as 'comprising' or 'having' are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It is to be understood that this does not preclude the possibility of the presence or addition of numbers, steps, operations, components, parts, or combinations thereof. When it is said that an element is 'connected' or 'connected' to another element, it is understood that it may be directly connected or connected to the other element, but other elements may exist in between. it should be On the other hand, when it is mentioned that a certain element is 'directly connected' or 'directly connected' to another element, it should be understood that the other element does not exist in the middle.

In addition, in describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be abbreviated or omitted.

The present invention provides a cosmetic composition containing a starfish extract and hydrolyzed collagen as active ingredients.

Hereinafter, the cosmetic composition according to the present invention will be described in detail.

The cosmetic composition comprises 1-5 parts by weight of a first extract comprising a starfish extract, a sea cucumber extract and a sea urchin extract in a weight ratio of 4:3:3; The second extract 1-5 comprising pine needle extract, lotus leaf extract, lotus seed extract, honeysuckle flower extract, propolis extract, centella asiatica extract and gilkyung extract in a weight ratio of 2.5:2:1.5:1:1:1:1 parts by weight; 0.1-1 parts by weight of hydrolyzed collagen; 4-8 parts by weight of hyaluronic acid; 3-7 parts by weight of butylene glycol; 1-5 parts by weight of glycerin; 1-5 parts by weight of shea butter; 1-5 parts by weight of propanediol; 0.1-1 parts by weight of tris(hydroxymethyl)aminomethane; Cholesterol 0.1-1 parts by weight; 0.1-1 parts by weight of jojoba oil; 0.1-1 parts by weight of rosemary leaf oil; 0.05-0.5 parts by weight of hydrogenated poly(C6-14 olefin); 0.05-0.5 parts by weight of carbomer; 0.1-1 parts by weight of polysorbate 80; 2-6 parts by weight of dicapryl carbonate; 0.5-4 parts by weight of 1,2-hexanediol; 3-7 parts by weight of dipropylene glycol; and 58-62 parts by weight of purified water.

The cosmetic composition more preferably contains 2-4 parts by weight of the first extract comprising a starfish extract, a sea cucumber extract and a sea urchin extract in a weight ratio of 4:3:3.

The starfish has a flat, star-shaped body, and is one of the marine animals commonly encountered in coastal waters. As a marine benthic species belonging to the echinoderm, it is distributed and inhabited in most oceans. It has been reported in academia that there are about 1,800 living species of these starfish, and it is reported that there are more than 200 species inhabiting the waters of Korea. Most of these starfish are about 20-30 cm in diameter, sometimes less than 1 cm, and sometimes as high as 65 cm. By including the starfish extract in the cosmetic composition, it has the effect of improving skin wrinkles, promoting collagen synthesis, and moisturizing.

The sea cucumber is a marine invertebrate belonging to the echinoderm family (holothuroidea), and 14 species of 4 families, including blue sea ginseng, black sea ginseng, and red sea ginseng, inhabit in Korea. Sea cucumber, along with ginseng, has been the subject of food and nutritional research since ancient times as a representative health food. Jasaneobo, Bonchogangmok, Donguibogam, etc., have been widely used since ancient times because of their effects on rejuvenation and nerve stabilization. The present invention provides a cosmetic composition comprising a sea cucumber extract, and the sea cucumber extract is preferably a sea cucumber intestine extract. By including the sea cucumber gut extract as a cosmetic composition, it has the effect of improving skin wrinkles, promoting collagen synthesis, and moisturizing.

The sea urchin belongs to the echinoderm, Class Echinoidea, and is mainly distributed from the coast to the deep water. Worldwide, it is distributed in the East Sea of Korea, the coast of China and Japan. Among the sea urchins produced in the seas near Korea, the sea urchins used for food are purple sea urchin (Anthocidaris crassispina), horse dung sea urchin (Hemicentrotus pulcherrimus), and pink sea urchin (Pseudocentrotus depressus). ), and the northern horse dung urchin (Strongylocentrotus internmedius). Sea urchin contains water, protein, fat, B vitamins and vitamin C, iron, magnesium and calcium, and contains more protein than sea cucumber. In the case of sea urchins, about 20% of the edible gonadal (egg) part is made up of sea urchin shells, and the remaining 80% is made up of sea urchin shells. Most of the degradable shells are left as they are. Furthermore, in recent years, sea algae is being depleted due to over-breeding of sea urchins, and bleaching occurs, which is emerging as an environmental problem that devastates the marine ecosystem. The present invention provides a cosmetic composition comprising a sea urchin extract, and the sea urchin extract is preferably a sea urchin roe extract. By including the sea urchin roe extract as a cosmetic composition, it has the effect of improving skin wrinkles, promoting collagen synthesis, and moisturizing.

The cosmetic composition comprises a pine needle extract, lotus leaf extract, lotus seed extract, honeysuckle flower extract, propolis extract, centella asiatica extract and gilgyeong extract in a weight ratio of 2.5:2:1.5:1:1:1:1. It is more preferable to include 2-4 parts by weight of the extract.

The pine needles are rich in terpene-based phytoncides to generate negative ions as such, and are a material containing a large amount of vitamins and minerals beneficial to the human body. In addition, pine needles provide a pine scent to the final composition to improve user satisfaction. The pine needle extract clears the skin by lowering the heat of the skin and has a soothing effect due to its unique sterilizing action.

The lotus leaf is also called a lotus (Nelumbo nucifera) as a perennial aquatic plant of the Lepidoptera family. It contains vitamin C and vitamin B12, as well as amino acids such as asparagine, arginine and tyrosine, which are effective in relieving fatigue and improving skin beauty. It has been known to be effective in bronchial asthma, gonorrhea, tonicity, indigestion, as well as snake and poisonous insect bites in folklore.

The lotus seed (Nelumbo Nucifera Seed) refers to the seed of the lotus flower. The lotus is a perennial plant of the Lepidoptera family, and the flowers bloom in July-August, are red or white, hang one at the end of the stalk, and there are seeds in the hole of the flower stalk. The safety of lotus has been proven as various parts of it have been used as food or herbal medicine for a long time. When this lotus seed extract is used as a cosmetic ingredient, it contains asparagine and arginine, so it can remove free radicals that are fatal to skin aging, and helps to make dull skin tone clear and bright. It can also help reduce oxidative stress on the skin by calming the skin and inhibiting peroxidation of the skin.

The honeysuckle flower (Lonicera Japonica Flower) refers to the flower of honeysuckle. It is a semi-evergreen broad-leaved vine that grows in mountains all over Korea. This honeysuckle has been generally used medicinally for high fever, acute hepatitis, and inflammation. When used as a cosmetic ingredient, this honeysuckle flower extract contains ingredients such as ginnol, sterols, glycosides, chlorogenic acid, flavonoid compounds, tannins, isochlorogenic acid, caffeic acid, and quercetin to enhance antioxidant activity and prevent skin aging.

The propolis is already a dark grayish-brown powder with no odor and a unique flavor, and the bees mix their saliva and enzymes with resin-like substances extracted from various plants for their survival and reproduction. As a manufactured material, organic substances and minerals (inorganic salts) are the most common ingredients. Minerals, vitamins, amino acids, fats, organic acids, flavonoids, etc. play an important role in cell metabolism, and terpenes have an anticancer effect. Its main effects include anti-inflammatory, antioxidant, immune enhancement, and moisturizing. When used as a cosmetic ingredient, this propolis extract contains more than 20 different flavonoids, maximizing its antioxidant role in protecting the skin from free radicals.

The Gilgyeong (Platycodon Grandiflorus), also called Gilgyeonggeun, is a medicinal material made by removing the root or epidermis of bellflower and bellflower, and is known to have a slight odor, bitter taste, and flat properties. Gilgyeong acts on the lungs to treat the symptoms of a lot of seawater and phlegm and difficulty breathing, and has the effect of stopping coughing and removing phlegm. It is also used when there is general edema due to inability to urinate and the amount of urine is small. Therefore, it is prescribed and used for sore throat, cough due to cold, phlegm, nasal congestion, asthma, bronchitis, pleurisy, headache, chills, and tonsillitis. As pharmacological action, expectorant action, cholesterol lowering action, and antibacterial inhibitory action have been reported. When used as a cosmetic ingredient, this gilkyung extract contains ingredients such as 3''-O-acetylplaticonic acid A, 3''-O-acetylpolygalaxin D, and polygalaxin D3 to inhibit the oxidation of cosmetics. Prevents deterioration, spoilage, and deterioration of cosmetics, and prevents the activity of external substances harmful to the skin.

The Centella asiatica is a slender perennial vine that grows wild in the tropics. It is also called tiger grass because it is said that tigers used to roll in the grass when their body was injured. In particular, the leaves and stems contain active ingredients such as asiaticoside, saponin, and matecassic acid, which helps to keep the skin smooth. give When this Centella asiatica extract is used as a cosmetic composition, it suppresses melanin, soothes irritated skin, prevents pigmentation, and aids in moisturizing effect.

The cosmetic composition more preferably contains 0.3-0.7 parts by weight of hydrolyzed collagen.

Hydrolyzed collagen is a collagen hydrolyzate obtained by treating or hydrolyzing raw pig or cow bones and skin with acids and enzymes, and consists of water-soluble peptides of various molecular weights. These peptides are rich in amino acids found in collagen, including L-glycine, L-proline and L-hydroxyproline. In general, it is known that when applied to hair preparations, it gives shine to the hair and increases elasticity, thereby improving the hair to be soft and full. In addition, as it is effective for bone and joint health, it is prescribed as a health functional food and distributed in the market. In the present invention, it is applied as a cosmetic composition to give a moisturizing effect to the skin, thereby reducing the occurrence of dead skin cells and restoring elasticity, thereby showing an effect on wrinkle improvement.

The cosmetic composition more preferably contains 5-7 parts by weight of hyaluronic acid.

Hyaluronic acid is one of glucosamine glycans (glycosaminoglycan). Hyaluronic acid, which consists of N-acetyl glucosamine and glucuronic acid, exists in the extracellular matrix and is involved in tissue moisture maintenance, cell growth factors and storage and diffusion of nutrients. It is known to be synthesized by keratinocytes and fibroblasts. A decrease in hyaluronic acid in the skin is known to be the cause of a decrease in skin elasticity and an increase in wrinkles. Therefore, maintaining the hyaluronic acid content in the skin plays an important role in moisturizing and maintaining skin elasticity, as well as improving wrinkles. In the present invention, it is applied as a cosmetic composition to exhibit skin moisturizing, skin elasticity maintenance and wrinkle improvement effects.

The cosmetic composition comprises 4-6 parts by weight of butylene glycol; 2-4 parts by weight of glycerin; 2-4 parts by weight of shea butter; 2-4 parts by weight of propanediol; 0.3-0.7 parts by weight of tris(hydroxymethyl) aminomethane; cholesterol 0.3-0.7 parts by weight; 0.3-0.7 parts by weight of jojoba oil; 0.3-0.7 parts by weight of rosemary leaf oil; 0.1-0.3 parts by weight of hydrogenated poly(C6-14 olefin); 0.1-0.3 parts by weight of carbomer; 0.3-0.7 parts by weight of polysorbate 80; 3-5 parts by weight of dicapryl carbonate; 1-3 parts by weight of 1,2-hexanediol; 4-6 parts by weight of dipropylene glycol; And 59-61 parts by weight of purified water; it is more preferable to include.

Also, the present invention

Washing the starfish, cut into appropriate sizes, freeze-drying, and pulverizing to prepare a starfish powder; Preparing an extract by mixing the starfish powder, Tris buffer solution, and a protein hydrolysis inhibitor and stirring at a temperature of 3-7° C. for 90-150 minutes; and centrifuging the extract to separate the supernatant and filtering it with a filter paper; preparing a starfish extract comprising;

Obtaining and washing sea cucumber intestines from sea cucumbers, cut into appropriate sizes, freeze-drying, and pulverizing to prepare sea cucumber intestines powder; extracting a solution containing the active ingredient by mixing the sea cucumber intestine powder with ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure; preparing a sea cucumber extract comprising;

Obtaining sea urchin roe from sea urchin, washing, cutting to an appropriate size, freeze-drying, and pulverizing to prepare sea urchin powder; extracting a solution containing an active ingredient by mixing the sea urchin powder with ethanol; and concentrating and lyophilizing the extracted solution under reduced pressure; preparing a sea urchin extract comprising;

Preparing a first mixture comprising pine needles, lotus leaves and lotus seeds in a weight ratio of 2.5:2:1.5 by washing pine needles, lotus leaves and lotus seeds, cut into appropriate sizes, freeze-dried, and pulverized; The first mixture, a mixed solvent of ethanol and acetone mixed in a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMIm][TFSI], 1 Extracting a solution containing an active ingredient by mixing -Ethyl-3-methylimidazolium bis (trifluoromethylsulfylimide); and concentrating and freeze-drying the extracted solution under reduced pressure; pine needle extract, lotus leaf extract and lotus seed extract, including Preparing a first natural extract comprising;

Honeysuckle flower, propolis, centella asiatica and gilts are washed, cut into appropriate sizes, freeze-dried, and pulverized to give honeysuckle flower powder, propolis powder, centella asiatica powder and gilkyong powder in a weight ratio of 1:1:1:1. Preparing a second mixture comprising; mixing the second mixture and water, performing agitation, and performing primary hot water extraction at a temperature of 115-125° C. for 6-12 hours; After performing the first hot water extraction, filtration with filter paper, and then freeze-drying the filtrate to prepare a first mixed extract; After performing the primary hot water extraction, filtering with filter paper, and then mixing the remaining solid with water again, performing secondary hot water extraction at a temperature of 85-95° C. for 20-28 hours; After the secondary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a second mixed extract; and mixing the first mixed extract and the second mixed extract; preparing a second natural extract including honeysuckle flower extract, propolis extract, centella asiatica extract and gilgyeong extract;

preparing a first extract by mixing the starfish extract, sea cucumber extract and sea urchin extract in a weight ratio of 4:3:3;

preparing a second extract by mixing the first natural extract and the second natural extract in a weight ratio of 3:2; and

1-5 parts by weight of the first extract; 1-5 parts by weight of the second extract; 0.1-1 parts by weight of hydrolyzed collagen; 4-8 parts by weight of hyaluronic acid; 3-7 parts by weight of butylene glycol; 1-5 parts by weight of glycerin; 1-5 parts by weight of shea butter; 1-5 parts by weight of propanediol; 0.1-1 parts by weight of tris(hydroxymethyl)aminomethane; Cholesterol 0.1-1 parts by weight; 0.1-1 parts by weight of jojoba oil; 0.1-1 parts by weight of rosemary leaf oil; 0.05-0.5 parts by weight of hydrogenated poly(C6-14 olefin); 0.05-0.5 parts by weight of carbomer; 0.1-1 parts by weight of polysorbate 80; 2-6 parts by weight of dicapryl carbonate; 0.5-4 parts by weight of 1,2-hexanediol; 3-7 parts by weight of dipropylene glycol; And 58-62 parts by weight of purified water; mixing; provides a method for preparing a cosmetic composition comprising a.

At this time, the manufacturing method of the cosmetic composition provided in one aspect according to the present invention is shown in a flowchart in FIG. 1 , and below, the manufacturing method of the cosmetic composition according to the present invention will be described in detail step by step with reference to FIG. 1 .

First, the method for preparing a cosmetic composition according to the present invention includes preparing a starfish extract, a sea cucumber extract and a sea urchin extract.

The step of preparing the starfish extract includes washing the starfish, cutting it to an appropriate size, freeze-drying, and pulverizing to prepare a starfish powder; Preparing an extract by mixing the starfish powder, Tris buffer solution, and a protein hydrolysis inhibitor and stirring at a temperature of 3-7° C. for 90-150 minutes; and centrifuging the extract to separate the supernatant and filtering with filter paper.

The Tris buffer solution, also referred to as a Tris buffer solution, is composed of a Tris-acetate buffer of pH 8.3 and ethylenediaminetetraacetic acid (EDTA) for sequestering divalent cations. The concentration of the Tris buffer solution is preferably 10 mM.

The proteolysis inhibitor is preferably phenyl methyl sulfonyl fluoride.

The step of preparing the sea cucumber extract includes: preparing a sea cucumber intestine powder by obtaining a sea cucumber intestine from a sea cucumber, washing, cutting it to an appropriate size, freeze-drying, and pulverizing; extracting a solution containing the active ingredient by mixing the sea cucumber intestine powder with ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure.

The step of preparing the sea urchin extract includes the steps of preparing sea urchin roe powder by obtaining sea urchin roe from sea urchin, washing, cutting to an appropriate size, freeze-drying, and pulverizing; extracting a solution containing an active ingredient by mixing the sea urchin powder with ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure.

Next, the method for preparing a cosmetic composition according to the present invention includes preparing a pine needle extract, a lotus leaf extract, a lotus seed extract, a honeysuckle flower extract, a propolis extract, a Centella asiatica extract and a Gilkyong extract.

The pine needle extract, lotus leaf extract and lotus seed extract are prepared at once, and are prepared as a first natural extract. In the step of preparing the first natural extract, pine needles, lotus leaves and lotus seeds are washed, cut into appropriate sizes, freeze-dried, and pulverized to obtain pine needle powder, lotus leaf powder and lotus seed powder in a weight ratio of 2.5:2:1.5. Preparing a first mixture comprising; The first mixture, a mixed solvent of ethanol and acetone mixed in a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMIm][TFSI], 1 -Ethyl-3-methylimidazolium bis (trifluoromethylsulfylimide) is mixed to extract a solution containing the active ingredient; and concentrating and freeze-drying the extracted solution under reduced pressure.

The honeysuckle flower extract, propolis extract, centella asiatica extract and gilgyeong extract are prepared at once, and are prepared as a second natural extract. In the step of preparing the second natural extract, honeysuckle flower, propolis, centella asiatica and gilgyeong are washed, cut into appropriate sizes, lyophilized, and pulverized to produce honeysuckle flower powder, propolis powder, centella asiatica powder and gilgyeong powder. preparing a second mixture comprising a 1:1:1:1 weight ratio; mixing the second mixture and water, performing agitation, and performing primary hot water extraction at a temperature of 115-125° C. for 6-12 hours; After performing the first hot water extraction, filtration with filter paper, and then freeze-drying the filtrate to prepare a first mixed extract; After performing the primary hot water extraction, filtering with filter paper, and then mixing the remaining solid with water again, performing secondary hot water extraction at a temperature of 85-95° C. for 20-28 hours; After the secondary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a second mixed extract; and a step of mixing the first mixed extract and the second mixed extract; it includes a honeysuckle flower extract, propolis extract, centella asiatica extract and gilgyeong extract, including.

Next, the manufacturing method of the cosmetic composition according to the present invention comprises the step of preparing the first extract by mixing the starfish extract, sea cucumber extract and sea urchin extract in a weight ratio of 4:3:3.

Next, the manufacturing method of the cosmetic composition according to the present invention comprises the step of preparing a second extract by mixing the first natural extract and the second natural extract in a weight ratio of 3:2.

Next, the manufacturing method of the cosmetic composition according to the present invention comprises: 1-5 parts by weight of the first extract; 1-5 parts by weight of the second extract; 0.1-1 parts by weight of hydrolyzed collagen; 4-8 parts by weight of hyaluronic acid; 3-7 parts by weight of butylene glycol; 1-5 parts by weight of glycerin; 1-5 parts by weight of shea butter; 1-5 parts by weight of propanediol; 0.1-1 parts by weight of tris(hydroxymethyl)aminomethane; Cholesterol 0.1-1 parts by weight; 0.1-1 parts by weight of jojoba oil; 0.1-1 parts by weight of rosemary leaf oil; 0.05-0.5 parts by weight of hydrogenated poly(C6-14 olefin); 0.05-0.5 parts by weight of carbomer; 0.1-1 parts by weight of polysorbate 80; 2-6 parts by weight of dicapryl carbonate; 0.5-4 parts by weight of 1,2-hexanediol; 3-7 parts by weight of dipropylene glycol; and 58-62 parts by weight of purified water.

Hereinafter, the present invention will be described in more detail by way of the following examples.

However, the following examples are merely illustrative of the content of the present invention, and the scope of the present invention is not limited by the examples and experimental examples.

<Preparation Example 1> Preparation of starfish extract

The starfish were washed, cut into appropriate sizes, lyophilized, and pulverized with a blender to prepare starfish powder. Tris buffer solution (10 mM Tris) and proteolysis inhibitor (phenyl methyl sulfonyl fluoride) 1 mM were mixed, and 1/5 of the starfish powder was added thereto based on the weight of the mixed solution, and the temperature was 4° C. for 120 minutes. An extract was prepared by stirring. After extraction, the extract was centrifuged to separate the supernatant and filtered through filter paper (0.45 μm syringe filter) to prepare a starfish extract.

<Preparation Example 2> Preparation of sea cucumber extract

Sea cucumber intestines were obtained from sea cucumbers, washed, cut into appropriate sizes, freeze-dried, and pulverized with a blender to prepare sea cucumber intestines powder. The sea cucumber viscera powder was mixed with 10-fold ethanol, extracted at a temperature of 30° C. for 12 hours, filtered with a filter paper, and concentrated under reduced pressure at 55° C. with a concentrator and freeze-dried to prepare a sea cucumber extract.

<Preparation Example 3> Preparation of sea urchin extract

Sea urchin roe was obtained from sea urchin, washed, cut to an appropriate size, lyophilized, and pulverized with a blender to prepare sea urchin powder. The sea urchin powder was mixed with 10 times ethanol, extracted at a temperature of 30° C. for 12 hours, filtered with a filter paper, and concentrated under reduced pressure at 55° C. with a concentrator and freeze-dried to prepare a sea urchin extract.

<Preparation Example 4> Preparation of the first natural extract

After preparing and washing pine needles, lotus leaves and lotus seeds, cut into appropriate sizes, freeze-dried, and grinded with a blender to prepare a mixture. In the mixture, pine needle powder, lotus leaf powder and lotus seed powder were mixed in a weight ratio of 2.5:2:1.5.

The mixture was mixed with a mixed solvent in which 8 times of acetone and chloroform were mixed in a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMIM ][TFSI], 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfylimide) was added by 1/20 of the weight of the mixture, followed by ultrasonic processing (ultra sonic) and extraction at 30°C for 12 hours, followed by filtration with filter paper Then, a first natural extract was prepared by concentrating under reduced pressure at 55° C. and freeze-drying with a concentrator.

<Preparation Example 5> Preparation of the second natural extract

Honeysuckle flower, propolis, centella asiatica, and gilgyeong are prepared, washed, cut into appropriate sizes, freeze-dried, and ground in a blender to prepare a mixture. In the mixture, honeysuckle flower powder, propolis powder, centella asiatica powder and gilgyeong powder were mixed in a weight ratio of 1:1:1:1.

The mixture was mixed with water 10 times, and heated to a temperature of 120° C. without stirring to perform primary hot water extraction for 9 hours. Then, it was filtered with filter paper, and the filtrate was freeze-dried to prepare a first mixed extract. The filtered solid was again mixed with water 10 times the weight of the solid, and heated to a temperature of 90° C. while stirring to perform secondary hot water extraction for 24 hours. Then, it was filtered with filter paper, and the filtrate was freeze-dried to prepare a second mixed extract. Finally, a second natural extract was prepared by mixing the first mixed extract and the second mixed extract.

<Preparation Example 6> Preparation of the first extract

A first extract was prepared by mixing 40 parts by weight of the starfish extract prepared in Preparation Example 1, 30 parts by weight of the sea cucumber extract prepared in Preparation Example 2, and 30 parts by weight of the sea urchin extract prepared in Preparation Example 3.

<Preparation Example 7> Preparation of the second extract

A second extract was prepared by mixing 60 parts by weight of the first natural extract prepared in Preparation Example 4 and 40 parts by weight of the second natural extract prepared in Preparation Example 5.

<Example 1> Preparation of cosmetic composition

A cosmetic composition was prepared using the first extract prepared in Preparation Example 6, the second extract prepared in Preparation Example 7, and the like. The names and contents of each component are shown in Table 1 below.

Ingredient name content (wt%) first extract 3 second extract 3 Hydrolyzed Collagen 0.5 hyaluronic acid 6 butylene glycol 5 glycerin 3 shea butter 3 propanediol 3 Tris (hydroxymethyl) aminomethane 0.3 cholesterol 0.4 jojoba oil 0.5 rosemary leaf oil 0.5 Hydrogenated Poly (C6-14 Olefin) 0.2 Carbomer 0.2 Polysorbate 80 0.4 Dicaprylic Carbonate 4 1,2-Hexanediol 2 dipropylene glycol 5 Purified water 60

<Experimental Example 1>

1. Skin wrinkle improvement effect

In order to confirm the skin wrinkle improvement effect of the cosmetic composition prepared in Example 1, the following experiment was performed. Specifically, each of the 30 test subjects in their 30s and 50s was tested for 30 days by spreading and applying about 3-4 g of the cosmetic composition to the face twice daily in the morning and in the evening. After completion of the 30-day test, the degree of wrinkle improvement was evaluated using the observer's naked eye. The degree of wrinkle improvement was evaluated in items 1-4 as follows, and the results are shown in Table 2 below.

No. 1: No wrinkle improvement at all

No. 2: Wrinkle improvement was achieved, but no significant change was felt.

No. 3: Excellent wrinkle improvement effect, feel change

No. 4: Remarkably excellent wrinkle improvement effect

Item Example 1 number 1 0 No.2 0 number 3 24 number 4 6

As shown in Table 2, it was confirmed that the wrinkle improvement effect was excellent.

2. Moisturizing effect

In order to confirm the moisturizing effect of the cosmetic composition prepared in Example 1, the following experiment was performed. Through a survey, 100 healthy adult men and women who felt that their skin was dry were randomly selected, and the cosmetic composition of Example 1 was applied to the face twice a day for 8 weeks, respectively, and then washed off with water. The moisturizing effect was evaluated every 1 week from the start of the actual use test, and the improvement effect after the end was evaluated using corneometer CM 820 (Corage +Khazaka, Germany), a moisture content measuring device. The measurement results of the moisturizing effect are shown in Table 3 below.

Example 1 before use 24.8 1 week after use 39.2 2 weeks after use 50.4

As shown in Table 3, it was confirmed that the moisturizing effect was excellent.

Claims (3)

1-5 parts by weight of the first extract comprising a starfish extract, a sea cucumber extract and a sea urchin extract in a weight ratio of 4:3:3; The second extract 1-5 comprising pine needle extract, lotus leaf extract, lotus seed extract, honeysuckle flower extract, propolis extract, centella asiatica extract and gilgyeong extract in a weight ratio of 2.5:2:1.5:1:1:1:1 parts by weight; 0.1-1 parts by weight of hydrolyzed collagen; 4-8 parts by weight of hyaluronic acid; 3-7 parts by weight of butylene glycol; 1-5 parts by weight of glycerin; 1-5 parts by weight of shea butter; 1-5 parts by weight of propanediol; 0.1-1 parts by weight of tris(hydroxymethyl)aminomethane; Cholesterol 0.1-1 parts by weight; 0.1-1 parts by weight of jojoba oil; 0.1-1 parts by weight of rosemary leaf oil; 0.05-0.5 parts by weight of hydrogenated poly(C6-14 olefin); 0.05-0.5 parts by weight of carbomer; 0.1-1 parts by weight of polysorbate 80; 2-6 parts by weight of dicapryl carbonate; 0.5-4 parts by weight of 1,2-hexanediol; 3-7 parts by weight of dipropylene glycol; And 58-62 parts by weight of purified water; Cosmetic composition comprising a. delete Washing the starfish, cut into appropriate sizes, freeze-drying, and pulverizing to prepare a starfish powder; Preparing an extract by mixing the starfish powder, Tris buffer solution, and a protein hydrolysis inhibitor and stirring at a temperature of 3-7° C. for 90-150 minutes; and centrifuging the extract to separate the supernatant and filtering it with a filter paper; preparing a starfish extract comprising;
Obtaining and washing sea cucumber intestines from sea cucumbers, cut into appropriate sizes, freeze-drying, and pulverizing to prepare sea cucumber intestines powder; extracting a solution containing the active ingredient by mixing the sea cucumber intestine powder with ethanol; and concentrating and freeze-drying the extracted solution under reduced pressure; preparing a sea cucumber extract comprising;
Obtaining sea urchin roe from sea urchin, washing, cutting to an appropriate size, freeze-drying, and pulverizing to prepare sea urchin powder; extracting a solution containing an active ingredient by mixing the sea urchin powder with ethanol; and concentrating and lyophilizing the extracted solution under reduced pressure; preparing a sea urchin extract comprising;
Preparing a first mixture comprising pine needles, lotus leaves and lotus seeds in a weight ratio of 2.5:2:1.5 by washing pine needles, lotus leaves and lotus seeds, cut into appropriate sizes, freeze-dried, and pulverized; The first mixture, a mixed solvent of ethanol and acetone mixed in a volume ratio of 1:1, and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMIm][TFSI], 1 Extracting a solution containing an active ingredient by mixing -Ethyl-3-methylimidazolium bis (trifluoromethylsulfylimide); and concentrating and freeze-drying the extracted solution under reduced pressure; pine needle extract, lotus leaf extract and lotus seed extract, including Preparing a first natural extract comprising;
Honeysuckle flower, propolis, centella asiatica and gilts are washed, cut into appropriate sizes, freeze-dried, and pulverized to give honeysuckle flower powder, propolis powder, centella asiatica powder and gilkyong powder in a weight ratio of 1:1:1:1. Preparing a second mixture comprising; mixing the second mixture and water, performing agitation, and performing primary hot water extraction at a temperature of 115-125° C. for 6-12 hours; After performing the first hot water extraction, filtration with filter paper, and then freeze-drying the filtrate to prepare a first mixed extract; After performing the primary hot water extraction, filtering with filter paper, and then mixing the remaining solid with water again, performing secondary hot water extraction at a temperature of 85-95° C. for 20-28 hours; After the secondary hot water extraction, filtering with filter paper and then freeze-drying the filtrate to prepare a second mixed extract; and mixing the first mixed extract and the second mixed extract; preparing a second natural extract including honeysuckle flower extract, propolis extract, centella asiatica extract and gilgyeong extract;
preparing a first extract by mixing the starfish extract, sea cucumber extract and sea urchin extract in a weight ratio of 4:3:3;
preparing a second extract by mixing the first natural extract and the second natural extract in a weight ratio of 3:2; and
1-5 parts by weight of the first extract; 1-5 parts by weight of the second extract; 0.1-1 parts by weight of hydrolyzed collagen; 4-8 parts by weight of hyaluronic acid; 3-7 parts by weight of butylene glycol; 1-5 parts by weight of glycerin; 1-5 parts by weight of shea butter; 1-5 parts by weight of propanediol; 0.1-1 parts by weight of tris(hydroxymethyl)aminomethane; Cholesterol 0.1-1 parts by weight; 0.1-1 parts by weight of jojoba oil; 0.1-1 parts by weight of rosemary leaf oil; 0.05-0.5 parts by weight of hydrogenated poly(C6-14 olefin); 0.05-0.5 parts by weight of carbomer; 0.1-1 parts by weight of polysorbate 80; 2-6 parts by weight of dicapryl carbonate; 0.5-4 parts by weight of 1,2-hexanediol; 3-7 parts by weight of dipropylene glycol; And 58-62 parts by weight of purified water; mixing; a method of manufacturing a cosmetic composition comprising a.

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