KR102330277B1 - Cosmetics composition comprising centella asiatica extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising an extract of Centella asiatica.
The cosmetic composition comprising Centella asiatica extract according to the present invention includes Centella asiatica extract, Hwangchil tree leaf extract, selenium, Cordyceps extract, raspberry extract, iris extract and jade powder.
According to the above configuration, the present invention comprises a cosmetic composition including Centella asiatica extract, thereby improving the skin, having an excellent skin moisturizing effect, improving skin whitening and skin elasticity, and improving skin wrinkles. can be manufactured.

Description

Cosmetic composition comprising Centella asiatica extract {COSMETICS COMPOSITION COMPRISING CENTELLA ASIATICA EXTRACTS}

The present invention relates to a cosmetic composition comprising Centella asiatica extract, and more particularly, by composing the cosmetic composition including Centella asiatica extract, it improves skin, has excellent skin moisturizing effect, and improves skin whitening and skin elasticity, and improves skin elasticity. It relates to a cosmetic composition comprising an extract of Centella asiatica that can improve wrinkles.

In modern society, active oxygen is generated in large quantities due to ultraviolet rays and water pollution due to exhaust gas, harmful chemicals, and destruction of the ozone layer. It is known as the main cause.

In addition, in recent years, outdoor leisure activities such as golf are increasing in order to relieve excessive social and work stress. And skin aging is unavoidable, which negatively affects the trend of enjoying outdoor leisure activities more actively and comfortably.

In general, the skin is responsible for the primary function of preventing harm from various external factors that impair the homeostasis of our body through basic metabolic processes. In other words, as the skin is located at the outermost part of the body, it performs various roles such as protection through physical, chemical and biological barrier functions, thermoregulation, sensory, secretion and excretion, absorption, storage, and regeneration. , most susceptible to damage when subjected to constant external stress.

The main causes of such external stress include UV exposure, exhaust gas, yellow sand, environmental pollutants, heavy metals, temperature changes, and microorganisms, which are mainly reactive oxygen species through oxidative stress. , ROS), disrupts the antioxidant defense system, causing cell damage and death.

In general, the phenomenon of skin wrinkling due to weakening of elasticity and contractility of skin cells is caused by various causes, but it is widely known that skin aging is due to deterioration of skin contraction and astringent action.

Skin aging is largely divided into intrinsic aging and extrinsic aging. Clinically, it is characterized by decreased elasticity, rough skin texture, deep wrinkles, and pigmentation.

In addition, external factor aging refers to an aging phenomenon caused by extrinsic factors such as ultraviolet rays, reactive oxygen species, and stress. Due to these factors, aging of the skin proceeds rapidly, and as a result, rough wrinkles are generated on the skin surface and elasticity is significantly reduced.

Various studies have been conducted to elucidate the aging mechanism of the skin as described above, and various causes of skin aging have been revealed by these studies. As the skin's physiological activity as a defense barrier is lowered, aging is accelerated, and when the skin is exposed to harmful reactive oxygen species or free radicals, lipid peroxide is generated by oxidation in the body, and various proteins constituting the skin are modified, causing the skin aging progresses.

This skin aging and elasticity decrease is caused by the reduction and destruction of collagen and elastin due to the weakening of skin cells (fibroblasts) caused by ultraviolet rays. Collagenase and elastase are expressed in the skin by ultraviolet rays, etc., and these enzymes decompose normal collagen and elastin in the skin, resulting in a decrease in skin elasticity.

On the other hand, Centella asiatica L Urban is a creeping perennial herb belonging to the Umbrella family that is native to the African island of Madagascar. In Korea, it grows wild on Jeju Island belonging to the temperate region and island regions in the southern region.

Centella asiatica has been used medicinally for a long time through traditional remedies in India and Asia, and its main ingredients, asiaticoside and madecassoside, are pentacyclic triterpene glycosides belonging to the alpha-amyrin-ursolic acid group, and show activity in treating skin wounds and chronic ulcers. In addition, these substances have been used to treat leprosy because they dissolve the waxy capsule of Mycobacterium leprae. Among them, asiaticoside, in particular, has antibacterial, antibacterial and anti-inflammatory activities, so it not only has skin wounds, stomach ulcers, but also various skin diseases, mental disorders, tuberculosis, and veins. It is known to be effective in the treatment of diseases and dementia.

Centella asiatica contains asiaticoside, madecassoside, asiatic acid, madecassic acid, vallerin, and trace amounts of alkaloids, so it is excellent for wound healing and has anti-dementia and gastrointestinal effects, so it is used variously as a raw material for treating diseases. In particular, these substances are substances having the ability to regenerate skin tissue and promote collagen production, and are used as main ingredients in ointments for wound healing purposes in actual clinical practice.

As such, Centella asiatica is a medicinal plant that is widely used as a skin treatment agent, wound healing agent, memory enhancer, and tonic agent.

Domestic Patent Publication No. 10-2019-0050394 (published on May 13, 2019) Domestic Patent Publication No. 10-2019-0024734 (published on March 08, 2019) Domestic Patent Publication No. 10-2017-0103172 (published on September 13, 2017)

The present invention provides a cosmetic composition comprising Centella asiatica extract, which can improve skin, have excellent skin moisturizing effect, enhance skin whitening and skin elasticity, and improve skin wrinkles by composing a cosmetic composition including Centella asiatica extract is to provide

Various problems to be solved by the present invention are not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

The cosmetic composition comprising Centella asiatica extract according to the present invention includes Centella asiatica extract, Hwangchil tree leaf extract, selenium, Cordyceps extract, raspberry extract, iris extract and jade powder.

The centella asiatica extract 1 to 5 parts by weight, hwangchil tree leaf extract 0.1 to 0.5 parts by weight, selenium 0.05 to 0.1 parts by weight, cordyceps extract 0.1 to 0.5 parts by weight, raspberry extract 0.02 to 0.06 parts by weight, iris extract 0.02 to 0.06 parts by weight And it may be included in a weight ratio of 0.01 to 0.05 parts by weight of jade powder.

The Centella asiatica extract is washed after preparing Centella asiatica, and the washed Centella asiatica is steamed with steam, wherein the steaming is performed at a temperature of 130 to 150° C. and ^{a pressure of 3 to 5 kgf/cm 2} for 10 to 30 minutes. It is carried out by heating with steam, but the steamed Centella asiatica is dried, wherein the drying is performed by drying Centella asiatica at a temperature of 18 to 22 ℃ and a humidity of 55 to 60% for 30 to 60 hours, and heating the dried Centella asiatica. After roasting, cooling, but the roasting is first roasting for 1 to 5 minutes at a temperature of 75 to 80 ℃ after putting the dried Centella asiatica into a heating container, and the first roasting Centella asiatica at a temperature of 120 to 140 ℃ The second roasting is performed for 10 to 50 seconds in a, and the second roasted Centella asiatica is cooled at a temperature of 35 to 45° C. for 1 to 3 hours, and the cooled Centella asiatica is inoculated by spraying mineral and lactic acid bacteria culture solution, Inoculated by spraying at a weight ratio of 1 to 3 parts by weight of minerals and 5 to 10 parts by weight of lactic acid bacteria culture solution with respect to 100 parts by weight of the cooled Centella asiatica, and the minerals include calcium, phosphorus, potassium, magnesium, manganese, iodine, selenium, molybdenum and Any one or more selected from the group consisting of cobalt is used, and the lactic acid bacteria use a lactic acid strain isolated from kimchi, and the lactic acid strain is Lactobacillus curvatus ( Lactobacillus curvatus ) , Weissella viridescens ) , Lactobacillus plantarum ( Lactobacillus plantarum ) and Any one or more lactic acid strains selected from the group consisting of Leuconostoc lactis are used, and the fermented Centella asiatica inoculated with the mineral and lactic acid bacteria culture medium, wherein the fermentation is 38 to After maintaining a temperature of 42° C. and a humidity of 60 to 62%, it is fermented for 3 to 5 days, and is prepared through a process of extracting the fermented Centella asiatica, and mixing the fermented bottlenose with a solvent to prepare a mixture After that, it is put into an ultrasonic extractor, ultrasonic waves are applied to the mixture, and the solids contained in the mixture are removed to prepare a Centella asiatica extract, and the solvent is water or an alcohol having 1 to 4 carbon atoms (C1 to C4) selected from alcohol. Any one or more solvents are used, and the ultrasonic waves applied to the mixture at a temperature of 18 to 20° C. and ^{a pressure of 0.5 to 1.0 kgf/cm 2} are 150 to 300 watts (watts) at a vibration frequency of 50 to 80 KHz for 150 to 200 minutes. ) can be extracted using the output of

Specific details of other embodiments are included in the detailed description.

According to the present invention, by composing a cosmetic composition including Centella asiatica extract, it is possible to prepare a cosmetic composition capable of improving skin, having excellent skin moisturizing effect, enhancing skin whitening and skin elasticity, and improving skin wrinkles.

It will be fully understood that embodiments of the technical idea of the present invention may provide various effects not specifically mentioned.

Advantages and features of the present invention, and a method of achieving them, will become apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed subject matter may be thorough and complete, and that the spirit of the present invention may be sufficiently conveyed to those skilled in the art.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Hereinafter, the cosmetic composition comprising the Centella asiatica extract according to the present invention will be described in detail with reference to preferred examples.

The cosmetic composition comprising the Centella asiatica extract according to the present invention includes the Centella asiatica extract.

As the Centella asiatica extract, the Centella asiatica extract prepared by the following preparation method may be used.

First, the centella can be prepared and then washed.

As the Centella asiatica contains asiaticoside, madecassoside, asiatic acid, madecassic acid, vallerin and trace amounts of alkaloids, it is excellent for wound treatment, and has anti-dementia and gastrointestinal effects, etc. In particular, these substances are substances having the ability to regenerate skin tissue and promote collagen production, and are used as main ingredients in ointments for wound healing purposes in actual clinical practice.

As such, Centella asiatica is a medicinal plant that is widely used as a skin treatment agent, wound healing agent, memory enhancer, and tonic agent.

Next, the washed Centella asiatica may be steamed with steam.

In the above step, the steaming may be performed by heating the washed Centella asiatica with steam at a temperature of 130 to 150° C. and ^{a pressure of 3 to 5 kgf/cm 2} for 10 to 30 minutes.

Next, the steamed Centella asiatica may be dried.

The drying in the above step is drying the Centella asiatica at a temperature of 18 to 22° C. and a humidity of 55 to 60% for 30 to 60 hours in order to prevent the chlorophyll of the Centella asiatica from being destroyed and the useful components from volatilizing during drying in sunlight. can do.

Subsequently, the dried Centella asiatica can be heated and roasted and then cooled, and the roasting is performed by first roasting the dried Centella asiatica for 1 to 5 minutes at a temperature of 75 to 80° C. The second roasting of the roasted Centella asiatica at a temperature of 120 to 140° C. for 10 to 50 seconds, and cooling the second roasted Centella asiatica at a temperature of 35 to 45° C. for 1 to 3 hours may proceed.

Next, the cooled centella asiatica can be inoculated by spraying the mineral and lactic acid bacteria culture solution.

In the above step, it can be inoculated by spraying at a weight ratio of 1 to 3 parts by weight of minerals and 5 to 10 parts by weight of the lactic acid bacteria culture solution with respect to 100 parts by weight of the cooled Centella asiatica, wherein the minerals and the lactic acid bacteria culture solution are included below the above-mentioned lower limit range. In this case, there may be a problem that sufficient fermentation is not made in the subsequent process, and if it is included in excess of the above-described upper limit range, there may be a problem in that the physical properties of Centella asiatica are deteriorated due to excessive fermentation.

In the above step, the mineral refers to a mineral that is absorbed by the Centella asiatica during the fermentation of the Centella asiatica in a later process and is a nutrient required in a relatively small amount for the growth, maintenance and reproduction of the human body, and is calcium, phosphorus, potassium, magnesium, and manganese. , any one or more selected from the group consisting of iodine, selenium, molybdenum and cobalt may be used.

In addition, in the step, the lactic acid bacteria can use a lactic acid strain isolated from kimchi, a fermented food, specifically, the lactic acid strain is Lactobacillus curvatus ( Lactobacillus curvatus ) , Weissella viridescens ) , Lactobacillus plantarum ( Lactobacillus plantarum ) and Leuconostoc lactis ( Leuconostoc lactis ) Any one or more known lactic acid strains selected from the group consisting of may be used.

For example, in the step, the pH of the medium is adjusted to 2.8-3.2 using 1.2N HCl to prepare a lactic acid bacteria culture solution from kimchi, and then the lactic acid strain is separated from the kimchi, and the separated lactic acid strain is used in MRS broth (Oxoid). , England), incubated for 20 to 25 hours at 37-39° C., ^{diluted to 1×1.0 8} to 5×1.0 ⁸ CFU/mL, and then centrifuged at 10,000 to 15,000 rpm for 10-15 minutes to obtain the supernatant. (supernatant) only, and after filtering the supernatant with a 0.45㎛ syringe filter (syringe filter), sterilized distilled water with respect to 100 parts by weight of the filtered supernatant by mixing and diluting in a weight ratio of 1,000 to 2,000 parts by weight of lactic acid bacteria A culture solution can be prepared.

Next, centella asiatica inoculated with the mineral and lactic acid bacteria culture solution may be fermented.

In the above step, the centella asiatica inoculated with the mineral and lactic acid bacteria culture medium is maintained at a temperature of 38 to 42 ° C and a humidity of 60 to 62%, and then fermented for 3 to 5 days. If it is carried out below the range, the problem may occur that the centella asiatica is not sufficiently fermented, and if it is carried out beyond the upper limit range, there may be a problem that the physical properties of the fermented centella asiatica are deteriorated.

Then, the fermented Centella asiatica may be extracted to prepare a Centella asiatica extract.

In the above step, for example, after preparing a mixture by mixing the fermented bottled extract with a solvent, it is put into an ultrasonic extractor to apply ultrasonic waves to the mixture, and the solids contained in the mixture are sieved. Centella asiatica extract can be prepared by removing using a sieve or the like, and as the solvent, any one or more solvents selected from water or alcohols having 1 to 4 carbon atoms (C1 to C4) may be used.

In addition, in the step, the ultrasonic waves applied to the mixture at a temperature of 18 to 20 ℃ and ^{a pressure of 0.5 to 1.0 kgf / cm 2} 150 to 300 watts (watt) output at a vibration frequency of 50 to 80 KHz for 150 to 200 minutes Centella asiatica extract can be prepared by extracting using

In addition, the cosmetic composition comprising the Centella asiatica extract according to the present invention includes, in addition to the Centella asiatica extract, Hwangchil tree leaf extract, selenium, Cordyceps extract, Raspberry Idaeus (Raspberry) Fruit Extract, Iris Ensata Extract, and jade It may further include powder.

In addition, the cosmetic composition comprising the Centella asiatica extract according to the present invention contains 1 to 5 parts by weight of Centella asiatica extract, 0.1 to 0.5 parts by weight of Hwangchil tree leaf extract, 0.05 to 0.1 parts by weight of selenium, 0.1 to 0.5 parts by weight of Cordyceps extract, 0.02 parts by weight of raspberry extract. to 0.06 parts by weight, 0.02 to 0.06 parts by weight of iris extract, and 0.01 to 0.05 parts by weight of jade powder may be included in a weight ratio.

The hwangchil leaf extract is prepared by extracting from the leaves of the hwangchil tree, and the hwangchil tree (Dendropanax morbifera Lev) is an evergreen broad-leaved arboreous tree of the genus Araliaceae and native to Korea that grows up to 15 m or more in height. Young twigs are green and glossy, flowers bloom in June, and 7-19mm long fruits ripen in black. It is a temperate plant that grows in areas where the minimum temperature is -2℃ or higher and the annual average temperature is 12~15℃ or higher.

Hwangchil tree secretes hwangchil, which is generally composed of 66.7% of non-volatile components, 10.8% of aromatic components, 8.1% of moisture, and 14.4% of solids, which are paint components that form a golden coating film. It is known to consist of β-cubebene, γ-selinene, and δ-cadinene of the quiterpenes. The aromatic components contained in Hwangchil have various pharmacological effects as well as their value as a benzoic incense that makes the mind and body clear and comfortable.

As the hwangchil tree leaf extract, the hwangchil tree leaf extract prepared by the following preparation method may be used.

First, in order to prepare a hwangchil tree leaf extract, it can be washed after preparing the hwangchil tree leaves.

Next, the washed hwangchil leaves can be pulverized to a certain particle size.

In the above step, by pulverizing the hwangchil tree leaves to a certain particle size, the active ingredient of the hwangchil tree leaves can be sufficiently extracted. . In the above step, when the particle size of the hwangchil leaves is pulverized to less than 500 μm, it takes a long time to pulverize, and the particles are too small to cause agglomeration between the particles in a later process, and when pulverized in excess of 2500 μm There may be a problem that it is difficult to effectively extract useful ingredients from the leaves of the hwangchil tree.

Next, it is possible to obtain a hwangchil tree leaf extract by removing the solid content after applying ultrasonic waves to the pulverized hwangchil tree leaves in an atmosphere of low temperature and reduced pressure.

In the above step, after preparing a mixture by mixing the pulverized hwangchil leaves with a solvent, it is put into an ultrasonic extractor to apply ultrasonic waves to the mixture, and the solids contained in the mixture are sieved through a known sieve such as a sieve. It is possible to prepare a hwangchil tree leaf extract by removing using Any one or more solvents selected from (C1 to C4) alcohols may be used.

In addition, in the step, the ultrasonic wave applied to the mixed solution at a temperature of 20 to 25° C. and ^{a pressure of 0.1 to 0.5 kgf/cm 2} is an output of 100 to 200 watts (watt) for 30 to 60 minutes at a vibration frequency of 30 to 50 KHz By extracting using a hwangchil tree leaf extract can be obtained.

The selenium is an essential trace element (mineral) in our body. This nutrient is an important component of antioxidant enzymes that protect cells from free radicals generated during normal oxygen metabolism, and is an essential component for the normal functioning of the immune system. have.

The selenium has a heavy metal detoxification action by excreting and detoxifying heavy metals such as mercury, cadnium, and lead accumulated in the human body, and also enhances immunity against virus infection, suppresses destruction of hepatocytes, and oxidative destruction of cells such as cancer and diabetes. It is effective in the prevention and treatment of diseases caused by In addition, selenium promotes metabolism, strengthens biological functions, and regenerates cells of each function of the human body and supplies nutrients to prevent aging by preventing and treating various diseases.

The Cordyceps extract is an extract obtained by freeze-drying the active ingredient of Cordyceps Cordyceps, which is a kind of Ascomycetes that form fruiting bodies (mushrooms) in insects. When analyzing the components of Cordyceps Cordyceps, 11 types of amino acids necessary for the human body and 10 types of amino acids with medicinal effects each accounted for more than 60% of the total content, and cordycepic acid, an isomer of quinic acid, was It contains about 7%.

The Cordyceps Cordyceps is also known for various pharmacological actions such as immunity enhancement, and various amino acids contained in Cordyceps Cordyceps are known to be effective in skin regeneration and inhibiting the formation of blemishes.

For example, the Cordyceps Cordyceps extract was first extracted with 15 liters of 75% (v/v) ethanol solution per 1.0 kg of Cordyceps Cordyceps at 80 to 85° C. for 4 hours, and then the secondary extraction was completed with 7 liters of the same solvent under the same conditions, Prepare a concentrate in a vacuum rotary evaporator until it becomes 1400 to 1600 ml, add water to the concentrate to dilute it by 2 to 3 times the weight, and centrifuge at 14,000 to 15,000 rpm for 40 to 60 minutes to completely remove the solid component, then , The collected supernatant is extracted twice with 1.5 times chloroform, the chloroform layer is removed, and the aqueous layer is extracted three times with 1.5 times the weight of ethyl acetate to prepare a Cordyceps cordyceps extract.

The raspberry extract (Rubus Idaeus (Raspberry) Fruit Extract) is prepared by extracting from the raspberry, the raspberry (Rubus Idaeus (Raspberry)) is a deciduous shrub with thorns, and the stem usually stands upright. The leaves are opposite, the flowers are white in spring, and there are 5 petals and sepals. The fruit is a cluster family, and ripens in summer, and the ripe fruit falls off the flower stalk.

The raspberry not only contains abundant fiber, but also contains a large amount of effective substances useful for physiological functions of the human body, such as vitamin C, manganese, vitamins A, B, calcium, and potassium.

The iris extract (Iris Ensata Extract) is prepared by extracting from the iris, the iris is a monocotyledonous perennial plant of the Liliaceae family, native to Europe and planted in many ponds, the rhizome is short and the beard root is yellowish brown. am. Flower stalks are branched, and the height is 60-100 cm. The leaves are about 1m long and 2-3cm wide.

The iris is used for dry stomach, soothing, pain relief, expectoration, antifungal, headache, blood circulation, rheumatism, anthelmintic, tonic, flavoring, etc. In particular, the root of iris is used for diarrhea, bronchitis, indigestion, etc.

The jade powder makes the skin texture soft and fine, and effectively delivers nutrients to the skin to improve the moisturizing effect. Jadeite is broadly classified into jadeite and nephrite, and jadeite is a lead pyroxene mineral belonging to the pyroxene group, and is a monoclinic material made of silicic acid, aluminum oxide, and soda. The hardness is a dense mass like crystal, and the light is black, cyan, green, transparent or translucent.

Nephrite is a monoclinic pyroxene mineral of inosilicate, and it is divided into nephrite and serpentine superbasic nephrite among high-earth marbles. It is determined by the degree of quality, and it is known that the finer the fiber, the better the quality.

Hereinafter, preferred examples and comparative examples for the cosmetic composition comprising the Centella asiatica extract according to the present invention will be described in more detail.

< Example 1 >

Centella asiatica extract 3% by weight, Hwangchil tree leaf extract 0.3% by weight, selenium 0.08% by weight, Cordyceps extract 0.3% by weight, raspberry extract 0.04% by weight, iris extract 0.04% by weight, jade powder 0.03% by weight and the remaining amount of purified water were mixed Cosmetics were prepared.

< Example 2 >

Centella asiatica extract 4% by weight, Hwangchil tree leaf extract 0.2% by weight, selenium 0.09% by weight, Cordyceps extract 0.1% by weight, raspberry extract 0.06% by weight, iris extract 0.03% by weight, jade powder 0.01% by weight and the remaining amount of purified water were mixed Cosmetics were prepared.

< Example 3 >

Centella asiatica extract 1% by weight, hwangchil tree leaf extract 0.5% by weight, selenium 0.05% by weight, cordyceps extract 0.4% by weight, raspberry extract 0.03% by weight, iris extract 0.06% by weight, jade powder 0.04% by weight and the remaining amount of purified water were mixed Cosmetics were prepared.

< Comparative Example 1 >

A cosmetic was prepared using the same composition as in Example 1, and in Comparative Example 1, a cosmetic was prepared without including centella asiatica extract, hwangchil tree leaf extract, selenium, and iris extract.

< Comparative Example 2 >

Cosmetics were prepared using the same composition as in Example 1, but in Comparative Example 2, cosmetics were prepared without including Centella asiatica extract, selenium, and Cordyceps extract.

1. Cosmetic stimulation experiment

The stimulation experiment for the cosmetic prepared as described above was carried out as follows.

First, the clinical trial was conducted on 20 healthy people (5 males, 15 females) between the ages of 10 and 50. After washing the face with soap, cosmetics according to Examples and Comparative Examples were applied without applying cosmetics or moisturizers After it was applied to the subject's face and dried, the skin condition (erythema, edema, papule, etc.) was observed 2 hours later, and qualitative evaluation was performed through the evaluation table as shown in [Table 1] below.

experimental item subjective score

degree of stimulation a: The skin becomes very red and the itchiness is strong. b: The skin becomes slightly red and there is itching c: The skin becomes slightly red and there is irritation. d: There is no change in skin condition, but there is slight irritation e: No irritation or change in skin condition

The evaluation results of the test subjects for the degree of stimulation in [Table 1] are shown in [Table 2] below.

experimental item subjective score Evaluation results Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 degree of stimulation a - - - - - b - - - - - c - - - - - d - - - - - e 20 20 20 20 20

As can be seen from [Table 2] above, it was confirmed that the cosmetics using the compositions according to Examples and Comparative Examples were safe substances with little irritation such as erythema and edema, as there was little irritation or skin condition change.

2. Formulation stability test

The formulation stabilization degree of the cosmetics prepared according to Examples 1, 2, and 3 was evaluated.

The evaluation of the formulation stabilization degree was carried out by confirming the phase stability by storing the cosmetic samples prepared according to Examples 1, 2, and 3 at a temperature of 40 ° C, room temperature, and -10 ° C, respectively, for 30 days, and the results are shown below. shown in [Table 5].

division Example 1 Example 2 Example 3 40℃ Good Good Good room temperature Good Good Good -20℃ Good Good Good

Referring to [Table 3], it was confirmed that the cosmetics prepared according to Examples 1, 2, and 3 had good formulation stability.

3. Antibacterial activity evaluation experiment

In order to evaluate the antimicrobial activity of the cosmetics prepared according to Example 1 and Comparative Example 1, the following experiment was performed. In the antibacterial activity evaluation experiment, Propionibacterium acnes ATCC 6919, an acne bacterium, was used as the test bacteria.

Acne bacteria were inoculated into each BHI broth and pre-cultured, and then inoculated at a concentration of 1% in 100 ml of sterile new BHI broth containing 5% of each composition, and then cultured in an anaerobic incubator for 2 days, and then the number of bacteria was measured. did.

division P. acnes Inoculum concentration (cfu/ml) Bacteria concentration after culture (cfu/ml) Example 1 2.32 x 10 ⁶ 2.45 x 10 ³ Comparative Example 1 2.32 x 10 ⁶ 5.88 x 10 ⁵

Referring to [Table 6], it can be seen that the cosmetic prepared according to Example 1 has significantly improved antibacterial activity against acne bacteria.

4. Skin elasticity and wrinkle improvement test

Skin elasticity and wrinkle improvement were evaluated in a survey conducted after applying the cosmetic prepared according to Example 1 to 20 women aged 20 to 50 on the face once a day for 2 weeks, and the results is shown in [Table 5] below.

The evaluation was evaluated on a 5-point scale of 1 to 5-point scale, and a higher score means better.

division before use after use skin moisturizing 2.3 4.0 skin elasticity 2.2 3.4 skin wrinkles 2.0 3.7 Skin blemishes, pigmentation 2.1 2.5 skin tone 2.0 3.2 pore 2.1 2.8

Referring to [Table 5], in a survey conducted after applying the cosmetic prepared according to Example 1 to the face once a day for 2 weeks, 16 of the experimenters reported skin moisturizing, skin elasticity, skin wrinkles, and skin Tone, etc. responded that improvement was improved, and among them, 8 responded that it improved significantly.

5. Visual evaluation of wrinkle improvement

In order to confirm the wrinkle improvement effect of the cosmetics according to Example 1 and Comparative Example 1, 20 Korean women aged 32 to 53 years were used in the same manner as in daily lotion use for each 8 weeks, and then the wrinkle improvement effect was observed. evaluated.

[Table 6] shown in

division Wrinkle improvement effect Effectiveness (%) Great slightly does not exist Example 1 14 6 - 70 Comparative Example 1 3 4 13 15

Referring to [Table 6], as in Example 1, cosmetics including Centella asiatica extract, Hwangchil tree leaf extract, selenium, Cordyceps extract, raspberry extract, iris extract, and jade powder are, as in Comparative Example 1, Centella asiatica extract, Hwangchil It was confirmed that wrinkle improvement was remarkably improved compared to cosmetics that did not contain tree leaf extract, selenium, or iris extract.

6. Measurement of moisturizing effect

The capacitance measurement method using a Corneometer CM820 (Courage, Germany) was used, and the moisturizing effect was compared and measured for the sample using Example 1 and Comparative Example 1.

For 15 women aged 30 to 50 years, just before the start of the test, the inner surface of the forearm was acclimatized in a constant temperature and humidity room for 20 minutes, and then skin moisture was maintained constant. Then, after measuring the initial moisture retention amount of each sample application site using a corneometer C820, the cosmetics prepared according to Example 3 and Comparative Example were applied.

The moisture increase/decrease rate was first measured 20 minutes after application, and the moisture increase/decrease rate was measured again after 2 hours. Each measurement site was repeatedly measured three times, and the skin moisture change rate was calculated according to the following [Equation 1] as the average value, and is shown in [Table 7].

[Equation 1]

Moisture change rate (%) = (Tdi-Tdo)-(Ntdi-NTdo) / ((Ntdi-Ntdo) + Tdo) * 100

Tdi: the nth measurement value of the sample application site

Tdo : Measured value before sample application

Ntdi : The nth measurement value of the area where the sample is not applied

NTdo : Measured value before application of the area where the sample is not applied

division Example 1 Comparative Example 1 20 minutes 32.05 6.54 120 minutes 17.56 2.12

Referring to [Table 7], as in Example 1, a cosmetic prepared including Centella asiatica extract, Hwangchil tree leaf extract, selenium, Cordyceps extract, raspberry extract, iris extract, and jade powder is Centella asiatica extract as in Comparative Example 1. , it was confirmed that the moisturizing effect was superior not only in the initial stage but also in the lapse of time, compared to cosmetics that did not contain extracts of , hwangchil tree leaf extract, selenium, and iris extract.

In the above, a preferred embodiment of the present invention has been described, but those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential features thereof. will be able Therefore, it should be understood that the embodiment described above is illustrative in all respects and not restrictive.

Claims (3)

Centella asiatica extract, Hwangchil tree leaf extract, selenium, Cordyceps extract, raspberry extract, iris extract and jade powder,
The centella asiatica extract 1 to 5 parts by weight, hwangchil tree leaf extract 0.1 to 0.5 parts by weight, selenium 0.05 to 0.1 parts by weight, cordyceps extract 0.1 to 0.5 parts by weight, raspberry extract 0.02 to 0.06 parts by weight, iris extract 0.02 to 0.06 parts by weight and jade powder in a weight ratio of 0.01 to 0.05 parts by weight,
The Centella asiatica extract is
After preparing Centella asiatica, it is washed, and the washed Centella asiatica is steamed with steam, wherein the steaming is ^{performed by heating the washed Centella asiatica with steam at a temperature of 130 to 150° C. and a pressure of 3 to 5 kgf/cm 2} for 10 to 30 minutes. The steamed Centella asiatica is dried, but the drying is dried Centella asiatica at a temperature of 18 to 22° C. and a humidity of 55 to 60% for 30 to 60 hours, and then cooled after heating the dried Centella asiatica. , The roasting is the first roasting for 1 to 5 minutes at a temperature of 75 to 80 ℃ after putting the dried Centella asiatica into a heating container, and 10 to 50 seconds for the first roasted Centella asiatica at a temperature of 120 to 140 ℃ During the second roasting, the second roasted Centella asiatica is cooled at a temperature of 35 to 45° C. for 1-3 hours, and the cooled Centella asiatica is inoculated by spraying minerals and lactic acid bacteria culture solution, but the cooled Centella asiatica 100 Inoculated by spraying at a weight ratio of 1 to 3 parts by weight of minerals and 5 to 10 parts by weight of the lactic acid bacteria culture medium, the minerals are calcium, phosphorus, potassium, magnesium, manganese, iodine, selenium, molybdenum and cobalt from the group consisting of is at least one selected is used, the lactic acid bacteria, but use of the lactic acid bacterial strains isolated from kimchi, with the lactic acid strain Lactobacillus ku Yerba tooth (Lactobacillus curvatus), Bar Sela cheated to sense (Weissella viridescens), Lactobacillus Planta Rum ( Lactobacillus plantarum ) and Any one or more lactic acid strains selected from the group consisting of Leuconostoc lactis are used, and the fermented Centella asiatica inoculated with the mineral and lactic acid bacteria culture medium, wherein the fermentation is 38 to After maintaining a temperature of 42° C. and a humidity of 60 to 62%, it is fermented for 3 to 5 days, and is prepared through a process of extracting the fermented Centella asiatica, and mixing the fermented bottlenose with a solvent to prepare a mixture After that, it is put into an ultrasonic extractor, ultrasonic waves are applied to the mixture, and the solids contained in the mixture are removed to prepare a Centella asiatica extract, and the solvent is water or an alcohol having 1 to 4 carbon atoms (C1 to C4) selected from alcohol. Any one or more solvents are used, and the ultrasonic waves applied to the mixture at a temperature of 18 to 20° C. and ^{a pressure of 0.5 to 1.0 kgf/cm 2} are 150 to 300 watts (watts) at a vibration frequency of 50 to 80 KHz for 150 to 200 minutes. ) A cosmetic composition comprising an extract of Centella asiatica, characterized in that it is extracted using the output. delete delete