KR102318361B1 - Compositon for antibacterial comprising natural extract and cosmetic composition including the same - Google Patents

Abstract

The present invention relates to an antibacterial preservative composition comprising a Shrubby sophora root extract, a pine leaf extract, and a mugwort leaf extract. The antibacterial preservative composition has antibacterial activities against one or more types of bacteria selected from the group consisting of Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Micrococcus luteus, and Staphylococcus aureus, and one or more types of fungi selected from the group consisting of Candida albicans and Aspergillus niger. Also, when the composition is used for mist, a spray angle is 55 to 75°, and is very excellent.

Description

Naturally-derived antibacterial preservative composition comprising ginseng root, pine leaf and mugwort leaf, and a cosmetic composition comprising the same

The present invention relates to an antibacterial preservative composition having antibacterial activity against bacteria and fungi and having an excellent spray angle when used for mist, and a cosmetic composition comprising the same.

As income levels rise, hygiene practices are becoming stricter, but food poisoning accidents are still the leading cause of death worldwide. Looking at the trend of food poisoning, recently, it occurs year-round regardless of the season, and the number of patients has not decreased, especially in winter.

In summer, various causative bacteria such as Salmonella, Staphylococcus aureus, and Vibrio enteritis are mainly detected, and in the winter of October-December, various causative bacteria are detected along with norovirus. Therefore, the Ministry of Food and Drug Safety has recently strengthened the standards for harmful microorganisms in food, and the role of antibacterial preservatives is becoming more important.

In addition, in the cosmetics and household products field, products contaminated with microorganisms have a risk of skin troubles such as skin rashes and inflammation and microbial infections as well as functional deterioration due to the deterioration of ingredients. The role of antimicrobial preservatives is very important.

Synthetic antibacterial preservatives are easy to use and have excellent antibacterial and antiseptic effects, so they are used in most foods, cosmetics, and household products. is becoming Problems with artificial antibacterial preservatives include skin irritation, allergy induction, cancer-causing potential (preservative component detection in breast cancer tissue), male fertility weakening, and many studies related to preservatives acting as female hormones), as well as possible central nerve paralysis and genetic mutation. The WHO and others are also warning about the risk of side effects.

Artificial antibacterial agents and preservatives that have been used for decades have such potential risks, so research on introducing antibacterial or preservatives of a different kind from the existing ones or finding substances with antibacterial or antiseptic effects among natural substances is being actively conducted. The microbial growth inhibitory effect of organic acids such as succinic, malic, tartaric, and benzoic acid contained in natural products and the antibacterial effect of flavonoids and catechins were studied. Protein substances such as lysozyme and transferrin contained in eggs, lactoferrin contained in milk, and nisin produced by lactic acid bacteria are known.

In addition, the antibacterial action of the ingredients of garlic and onion, which are widely used spices in Korea, has been studied, and the antibacterial properties of various domestic herbal or vegetable ingredients have been studied. It was reported that the extract had strong antibacterial properties against both Gram-positive and negative bacteria, and it was reported that the extract of the epiphysis inhibited the proliferation of Listeria monocytogenes. .

However, most natural antibacterial preservatives have a small antibacterial spectrum and weak antibacterial activity compared to synthetic preservatives, making it difficult to use widely. , In addition, there is also a possibility that the antimicrobial activity may be reduced due to oxidation or destruction of the active ingredient.

Representative plant-derived materials include many materials such as grapefruit extract, green tea extract, astragalus extract, garlic extract, and lemon extract, but they have limitations in natural materials such as color odor, stability, and formulation problems.

Therefore, there is a demand for an antibacterial preservative containing a natural substance that is stable and is rather helpful for formulation without generating a characteristic fragrance of a natural product.

Republic of Korea Patent Publication No. 2021-0049227 Republic of Korea Patent Publication No. 2021-0044762

An object of the present invention is to provide an antibacterial preservative composition having antibacterial activity against bacteria and fungi.

Another object of the present invention is to provide an antimicrobial preservative composition for cosmetics that can be used in a cosmetic composition having antibacterial activity against bacteria and fungi.

In addition, another object of the present invention is to provide an antibacterial preservative composition for mist that has antibacterial activity against bacteria and fungi and has an excellent spray angle when used for mist.

In addition, another object of the present invention is to provide a cosmetic composition containing the antibacterial preservative composition.

The antibacterial preservative composition of the present invention for achieving the above object may include a ginseng root extract, a pine leaf extract and a mugwort leaf extract as active ingredients.

The antimicrobial preservative composition may be included in an amount of 20 to 60 parts by weight of pine leaf extract and 20 to 60 parts by weight of mugwort leaf extract based on 100 parts by weight of ginseng root extract.

The antibacterial preservative composition may be mixed with a pine leaf extract and mugwort leaf extract in a weight ratio of 1: 1-3.

The ginseng root extract, pine leaf extract, and mugwort leaf extract may each be extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, ethylene glycol, ethyl ether, or a mixed solvent thereof.

The lower alcohol having 1 to 4 carbon atoms may be 20 to 80% methanol, ethanol, butanol or propanol.

The antibacterial preservative composition includes Escherichia coli , Klebsiella pneumoniae , Pseudomonas aeruginosa , Micrococcus luteus , Staphylococcus aureus , one or more bacteria selected from the group consisting of; And Candida albicans ( Candida albicans ), black mold ( Aspergillus niger ) It may have antibacterial activity against one or more fungi selected from the group consisting of.

In addition, the antimicrobial preservative composition for a cosmetic of the present invention for achieving the above other object is a natural extract comprising a ginseng root extract, a pine leaf extract and a mugwort leaf extract; And butylene glycol, pentylene glycol, a naturally-derived polyhydric alcohol comprising at least one selected from the group consisting of glycerin and glyceryl caprylate; may include.

The composition comprises 20 to 60 parts by weight of pine leaf extract, 20 to 60 parts by weight of mugwort leaf extract, 80 to 150 parts by weight of butylene glycol, 400 to 800 parts by weight of pentylene glycol, and 100 parts by weight of glycerin based on 100 parts by weight of ginseng root extract. to 400 parts by weight and 300 to 700 parts by weight of glyceryl caprylate may be included.

The pentylene glycol and butylene glycol may be mixed in a weight ratio of 1: 0.01-0.5.

In addition, the antibacterial preservative composition for mist of the present invention for achieving the above another object is a natural extract comprising a ginseng root extract, a pine leaf extract and a mugwort leaf extract; And butylene glycol, pentylene glycol, a naturally-derived polyhydric alcohol comprising at least one selected from the group consisting of glycerin and glyceryl caprylate; may include.

The spray angle of the antibacterial preservative composition for mist may be 55 to 75 °.

In addition, the cosmetic composition of the present invention for achieving the above another object may contain the antibacterial preservative composition.

The natural antibacterial preservative composition of the present invention has excellent antibacterial activity by using together the ginseng root extract, the pine leaf extract and the mugwort leaf extract, and has an excellent spray angle by using butylene glycol and pentylene glycol mixed in an optimal ratio. It can also be used in a cosmetic composition for mist.

The natural antibacterial preservative composition of the present invention is made of all natural ingredients, so it is safe for the human body, does not irritate the skin, and has a stable antibacterial effect, so that it can be usefully used without limitation in the food, household goods and cosmetics industries.

1 shows the antibacterial effect of the antimicrobial preservative composition prepared according to Example 1 of the present invention.

The present invention relates to a natural antibacterial preservative composition having antibacterial activity against bacteria and fungi and having an excellent spray angle when used for mist, and a cosmetic composition comprising the same.

The bacteria may include one or more selected from the group consisting of Escherichia coli , Klebsiella pneumonia , Pseudomonas aeruginosa , Micrococcus luteus , and Staphylococcus aureus. And, the fungus may include at least one selected from the group consisting of Candida albicans and Aspergillus niger.

In the regulations on the standards for natural and organic cosmetics announced by the Ministry of Food and Drug Safety, the raw materials that can be used for the manufacture of natural and organic cosmetics are natural raw materials, naturally derived raw materials, water, betaine, and carrageenan. , Lecithin and Lecithin derivatives, Tocopherol, Tocotrienol, Oryzanol, Annatto, Carotenoids / Xanthophylls, ConAbsolutes, Absolutes ), Resinoids, Lanolin, Phytosterol, Glycosphingolipids, Glycolipids, Xanthan Gum, Alkyl Betaine, Benzoic Acid and Benzoic Acid and its salts), Benzyl Alcohol, Salicylic Acid and its salts, Sorbic Acid and its salts, Dehydroacetic acid and its salts Acid and its salts), denatonium benzoate, tert-butyl alcohol, other denaturants (excluding phthalates), isopropylalcohol and tetrasodium glutamate diacetate. Synthetic raw materials cannot be used in the manufacture of natural and organic cosmetics. However, raw materials that are necessary for the quality or safety of natural cosmetics or organic cosmetics but are difficult to replace in nature may be used within 5%. Even in this case, the petrochemical fraction cannot exceed 2%.

As described above, natural cosmetics have limitations in the substances that can be used, so it is difficult to obtain natural preservatives and natural cosmetics having the desired effect, so synthetic raw materials are generally used. The desired effect was achieved by using a polyhydric alcohol.

Hereinafter, the present invention will be described in detail.

The antibacterial preservative composition of the present invention includes a ginseng root extract, a pine leaf extract, and a mugwort leaf extract as active ingredients.

The above-mentioned old ginseng is a perennial herb that grows wild in sunny mountains and fields in Korea, Siberia, and China. It has a bitter taste, and its efficacy is similar to that of ginseng.

In the present invention, as using ginseng root, the ginseng root extract can be extracted by an extraction method such as hot water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction, etc. using an extraction solvent.

Preferably, the ginseng root and the extraction solvent are mixed in a weight ratio of 1: 5 to 25, more preferably 1: 8 to 15, and extracted at 60 to 100 ° C. for 3 to 15 hours, more preferably 5 to 10 hours. After aging at 5 to 10 °C for 7 to 10 days, filtration and concentration under reduced pressure to obtain an extract. If the aging process is not performed after extraction, the unique scent of ginseng root is generated, and even when used with other extracts, the antibacterial activity may be lowered.

When the weight ratio of the ginseng root and the extraction solvent is out of the above range, the active ingredient of the ginseng root may be extracted in a small amount in the extract.

When the extraction temperature and extraction time are less than the above lower limits, the active ingredient of ginseng root may be extracted in a small amount, and the antibacterial effect may be reduced.

The extraction solvent for extracting the extract is water, lower alcohol having 1 to 4 carbon atoms, butylene glycol, ethylene glycol, ethyl ether, or a mixed solvent thereof. The lower alcohol may include 20 to 80 wt% of methanol, ethanol, butanol or propanol.

The extraction solvent is not particularly limited, but the extract extracted with 20 to 80% by weight of an aqueous ethanol solution preferably acts on the antibacterial effect.

In addition, it is known that the pine leaves contain a natural bactericidal component called phytoncide having an excellent antibacterial effect.

The pine leaf extract of the present invention is obtained by the same extraction method as the ginseng root extract, and when the weight ratio of the pine leaf and the extraction solvent is out of the above range, the active ingredient of the pine leaf may be extracted in a small amount in the extract. In addition, if the aging process is not performed after extraction, an unfavorable fragrance may be generated when mixed with the ginseng root extract, and the antibacterial activity may be lowered even when used with other extracts.

When the extraction temperature and extraction time are less than the lower limit, the active ingredient of the pine leaf may be extracted in a small amount, and the antibacterial effect may be reduced.

The pine leaf extract is used in an amount of 20 to 60 parts by weight, preferably 30 to 50 parts by weight, based on 100 parts by weight of the ginseng root extract. If the content of the pine leaf extract is less than the lower limit, the unique flavor of the ginseng root extract may not be removed and there may be no antibacterial activity, and if it exceeds the upper limit, the antimicrobial activity may be reduced.

In addition, the mugwort leaf purifies the blood, helps blood circulation, and is effective for sterilization, pain relief, anti-inflammatory, cold, lobster, menstrual pain, and the like.

The mugwort leaf extract of the present invention is obtained by the same extraction method as that of the ginseng root extract, and when the weight ratio of the mugwort leaf to the extraction solvent is out of the above range, the extract can be extracted with a small amount of the active ingredient of the mugwort leaf. In addition, if the aging process is not performed after extraction, the unique scent of mugwort may be generated and there may be little antibacterial activity.

When the extraction temperature and extraction time are less than the lower limit, the active ingredient of mugwort leaf may be extracted in a small amount, and the antibacterial effect may be reduced.

The mugwort leaf extract is used in an amount of 20 to 60 parts by weight, preferably 30 to 50 parts by weight, based on 100 parts by weight of the ginseng root extract. If the content of mugwort leaf extract is less than the lower limit, the unique flavor of the ginseng root extract may not be removed and antibacterial activity may be absent.

The pine leaf extract and mugwort leaf extract are mixed in a weight ratio of 1: 1-3, preferably 1: 1-2, and more preferably 1:1. Based on the pine leaf extract, when the content of mugwort leaf extract is less than the lower limit, the antibacterial activity may be reduced. odor may occur.

In addition, the present invention can provide an antibacterial preservative composition for cosmetics comprising a natural extract including a ginseng root extract, a pine leaf extract and an mugwort leaf extract, and a naturally-derived polyhydric alcohol and purified water.

The ginseng root extract, pine leaf extract, and mugwort leaf extract were described above, and thus were not further described.

The naturally-derived polyhydric alcohol may include at least one selected from the group consisting of butylene glycol, pentylene glycol, glycerin and glyceryl caprylate. The naturally-derived polyhydric alcohol is a naturally-derived ingredient certified by COSMOS.

When using the ginseng root extract, pine leaf extract and mugwort leaf extract, the spray angle is narrowed when pumping, so it cannot be used in the cosmetic composition for mist, but butylene glycol and pentylene glycol mixed in an optimal ratio By using it, it shows an excellent spray angle and can be used in a cosmetic composition for mist.

The butylene glycol is a component obtained from fatty acids extracted mainly by fermenting sugar cane, and has excellent moisturizing effect. Moreover, the butylene glycol of the present invention can be used as a cosmetic composition for mist by having an excellent spray angle when used in a specific amount together with pentylene glycol.

When only butylene glycol or pentylene glycol is used, the spray angle is reduced, so that a cosmetic composition for mist cannot be prepared.

The content of butylene glycol is 80 to 150 parts by weight, preferably 100 to 120 parts by weight, based on 100 parts by weight of the ginseng root extract. When the content of butylene glycol is less than the lower limit, the moisturizing power is weak and the viscosity is high, so it may be inconvenient to use.

In addition, the pentylene glycol is a skin conditioning agent and has antibacterial activity against fungi and the like.

The content of pentylene glycol is 400 to 800 parts by weight, preferably 500 to 700 parts by weight, based on 100 parts by weight of the ginseng root extract. If the content of pentylene glycol is less than the lower limit, the antibacterial activity may be reduced and the quality of the cosmetic composition may be deteriorated.

The pentylene glycol and butylene glycol are mixed in a weight ratio of 1: 0.01-0.5, preferably 1: 0.1-0.3 by weight. If the content of butylene glycol based on pentylene glycol is less than the lower limit, the spraying distance may be too short, and if it exceeds the upper limit, the spraying angle becomes narrow and it may be difficult to use as a mist.

The glycerin is a moisturizing agent that prevents moisture loss and prevents drying, and may have a moisturizing effect when used in the manufacture of cosmetics or soap.

The content of glycerin is 100 to 400 parts by weight, preferably 150 to 250 parts by weight, based on 100 parts by weight of the ginseng root extract. If the content of glycerin is less than the lower limit, the moisturizing effect may be lowered, and if it exceeds the upper limit, the effect may not be improved and the feel may be reduced.

The glyceryl caprylate is an auxiliary emulsifier produced from various vegetable oils, and restores the skin's own oil and controls the moisture of the skin to perform a humidifying role.

The content of glyceryl caprylate is 300 to 700 parts by weight, preferably 400 to 500 parts by weight, based on 100 parts by weight of the ginseng root extract. If the content of glyceryl caprylate is less than the lower limit, it may not be possible to control the moisture of the skin, and if it exceeds the upper limit, the effect may no longer be improved and the quality may be deteriorated.

In addition, the purified water is used in an amount of 400 to 800 parts by weight based on 100 parts by weight of the ginseng root extract.

According to the test method of the Korean Cosmetics Association (ASTM D 4041), when measuring the spray angle at the time of pump, it is said that if the spray angle is 55 ° or more, it is wide. It has a very wide angle of 55 to 75 degrees.

As used herein, the term 'extract' includes not only the crude extract obtained by treating the extraction solvent, but also the processed product of the extract. For example, the ginseng root extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

Meanwhile, in the present specification, the term 'contained as an active ingredient' means including an amount sufficient to achieve the efficacy or activity of the fermented product. For example, the extract is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml. Since the extract is a natural product and there is no side effect to the human body even when used in excess, the upper limit of the quantity of the extract contained in the composition of the present invention can be selected and carried out by those skilled in the art within an appropriate range.

In the cosmetic composition of the present invention, in addition to the cosmetic composition described above, other ingredients commonly formulated in cosmetics may be blended as necessary. Examples of such blending ingredients include oil and fat ingredients, moisturizing agents, emollients, surfactants, organic and inorganic pigments, Organic powder, UV absorber, preservative, disinfectant, antioxidant, pH adjuster, alcohol, colorant, fragrance, blood circulation promoter, cooling agent, limiting agent, purified water, water-soluble vitamin, fat-soluble vitamin, polymer peptide, polymer polysaccharide, sphingolipid and seaweed an extract, etc. are mentioned.

The cosmetic composition of the present invention may be prepared in the form of emulsified formulations and solubilized formulations commonly used in the art.

In addition, the components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the above components as active ingredients, for example, conventional adjuvants such as stabilizers, pigments and natural fragrances; It may further include a carrier.

Products to which the composition of the present invention can be added include, for example, mist, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, sunscreen cream , moisture cream, hand cream, foundation, essence, nourishing essence, mask pack, press powder, loose powder, eye shadow, etc., and soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane , butane or propellants such as dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

Hereinafter, preferred examples are presented to aid the understanding of the present invention, but the following examples are merely illustrative of the present invention and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

Example 1.

Ginseng Root Extract

After mixing ginseng root and 65% by weight of an aqueous ethanol solution in a weight ratio of 1:6, extraction was performed at 80°C for 5 hours, filtered, and the filtered extract was aged at 7°C for 8 days, filtered and concentrated under reduced pressure. By doing so, a ginseng root extract was obtained.

pine leaf extract

It was carried out in the same manner as in the method and conditions for preparing the ginseng root extract, but a pine leaf extract was obtained using pine leaves instead of ginseng root.

mugwort leaf extract

It was carried out in the same manner as in the method and conditions for preparing the ginseng root extract, except that mugwort leaves were used instead of ginseng root to obtain a mugwort leaf extract.

Antimicrobial Preservative Composition

100 parts by weight of ginseng root extract, 40 parts by weight of pine leaf extract, 40 parts by weight of mugwort leaf extract, 100 parts by weight of butylene glycol, 600 parts by weight of pentylene glycol, 200 parts by weight of glycerin, 400 parts by weight of glyceryl caprylate and purified water 520 parts by weight were mixed to obtain an antimicrobial preservative composition.

Comparative Example 1. Ginseng root extract

An antibacterial preservative composition was obtained in the same manner as in Example 1, except that the pine leaf extract and mugwort leaf extract were not used, and only the ginseng root extract was used.

Comparative Example 2. Mugwort leaf extract

An antibacterial preservative composition was obtained in the same manner as in Example 1, except that the ginseng root extract and the pine leaf extract were not used, and only the mugwort leaf extract was used.

Comparative Example 3. Pine leaf extract

An antibacterial preservative composition was obtained in the same manner as in Example 1, except that the ginseng root extract and mugwort leaf extract were not used and only the pine leaf extract was used.

Comparative Example 4. Content difference

An antibacterial preservative composition was obtained using the same procedure as in Example 1, except that 100 parts by weight of the ginseng root extract, 250 parts by weight of the pine leaf extract, and 250 parts by weight of the mugwort leaf extract were used.

Comparative Example 5. Pine leaf extract: mugwort leaf extract = 1: 5 weight ratio

An antibacterial preservative composition was obtained in the same manner as in Example 1, except that 20 parts by weight of pine leaf extract and 100 parts by weight of mugwort leaf extract were used.

Comparative Example 6. Butylene glycol content increase

It was carried out in the same manner as in Example 1, except that 200 parts by weight of butylene glycol was used to obtain an antibacterial preservative composition.

Comparative Example 7. Pentylene glycol: butylene glycol = 1:1 weight ratio

An antibacterial preservative composition was obtained in the same manner as in Example 1, except that 100 parts by weight of pentylene glycol and 100 parts by weight of butylene glycol were used.

<Test Example>

Test Example 1. Measurement of antibacterial activity

Antimicrobial activity was evaluated using the Microbial Challenge Test presented by the European Pharmacopoeia Commission (E.P.) in 1996 for topical formulations (Letters in Applied Microbiology 2002, 35, 385-389).

After preparing a bacterial solution by mixing equal amounts of bacteria and fungi, 1 ml of the prepared bacterial solution was added to 100 g of the sample.

TSA is a bacterial solution obtained by mixing the bacteria Escherichia coli , Klebsiella pneumoniae , Pseudomonas aeruginosa , Micrococcus luteus , and Staphylococcus aureus in equal amounts, respectively.

PDA is a bacterial solution in which the fungi Candida albicans and Aspergillus niger are mixed in equal amounts.

The antibacterial effect was observed from immediately after inoculation to 14 days, and the experimental results are shown in Table 1 below.

division Number of viable cells (cfu/ml) Day 0 Day 2 Day 7 Day 14 Example 1 TSA 8.9X10 ⁶ 1.1X10 ² ND ND PDA 4.5X10 ⁵ 3.7X10 ² ND ND Comparative Example 1 TSA 8.9X10 ⁶ 8.0X10 4.1X10 ³ 3.9X10 ³ PDA 4.5X10 ⁵ 1.6X10 ² 2.3X10 ³ 1.7X10 ³ Comparative Example 2 TSA 8.9X10 ⁶ 4.5X10 ³ 5.2X10 ³ 4.4X10 ³ PDA 4.5X10 ⁵ 2.2X10 ³ 5.0X10 ³ 2.0X10 ³ Comparative Example 3 TSA 8.9X10 ⁶ 4.5X10 ³ 4.2X10 ³ 4.0X10 ³ PDA 4.5X10 ⁵ 2.7X10 ³ 2.2X10 ³ 1.9X10 ³ Comparative Example 4 TSA 8.9X10 ⁶ 3.8X10 ³ 5.6X10 ND PDA 4.5X10 ⁵ 4.1X10 ³ 2.8X10 ND Comparative Example 5 TSA 8.9X10 ⁶ 4.3X10 ⁴ 5.3X10 ³ 2.9X10 ² PDA 4.5X10 ⁵ 4.7X10 ³ 7.2X10 ² 2.1X10 ² Comparative Example 6 TSA 8.9X10 ⁶ 4.3X10 ² 2.2X10 ND PDA 4.5X10 ⁵ 5.8X10 ² 4.3X10 ND Comparative Example 7 TSA 8.9X10 ⁶ 4.5X10 ² 2.6X10 ND PDA 4.5X10 ⁵ 5.5X10 ² 4.0X10 ND

As shown in Table 1 above, it was confirmed that the antibacterial preservative composition prepared according to Example 1 of the present invention had superior antibacterial activity against both bacteria and fungi compared to Comparative Examples 1 to 7.

It was confirmed that the antibacterial preservative compositions of Comparative Examples 1 to 3 using only one of the three extracts had a very low antibacterial activity.

Test Example 2. Antibacterial activity disc test

In order to see the antibacterial activity of the composition prepared according to Example 1, the clear zone was measured by the paper disc method. Escherichia coli , Klebsiella pneumonia , Micrococcus luteus , and Staphylococcus aureus were used as strains used to measure the antibacterial effect. After sterilization, each of the four types of bacteria was inoculated into tryptic soy agar cooled to 45 ° C so that the bacteria became 1x10 ⁶ cfu/ml, and then poured about 15 ml onto a 100 mm plate to prepare a solid medium. After placing an 8 mm antibiotic assay filter on the surface of the solid medium, 20 ul each of water and the composition of Example 1 of the present invention were treated on an 8 mm paper disc by concentration, and the bacteria were set at 35 ° C for 24-48 hours. After culturing in the incubator, the diameter of the clear zone was measured.

1 shows the antibacterial effect of the antimicrobial preservative composition prepared according to Example 1 of the present invention.

As shown in FIG. 1, it was confirmed that the antibacterial preservative composition prepared according to Example 1 had high antibacterial activity compared to Blank and water, and in particular, the higher the concentration (1 to 2% by weight), the more excellent the antibacterial effect. Confirmed.

Test Example 3. Injection angle measurement

Using the antibacterial preservative compositions prepared according to Example 1 and Comparative Examples 1 to 7 of the present invention, the spray angle was measured according to the test method (ASTM D 4041) of the Korean Cosmetics Association.

In the test method, after fixing the target paper to the supporter so that it is horizontal, pump 4 times until the sample is completely sprayed, and then place the pump container at an appropriate distance (152 mm) from the target paper. It is parallelized and pumped at a rate of 60 to 120 strokes/min.

The injection angle is calculated using the injection radius and the injection distance according to Equation 1 below.

[Equation 1]

Injection angle (ㅀ) = 2 tan ^-1 (D/(2XL))

D: Spray counterattack (diameter)

L: injection distance

The criterion for the judgment of the spray angle is 35° or less, Narrow, 45±10°, Normal, and 55° or more, Wide.

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 spray angle
(°) 65.1 40.2 45.2 40.0 50.2 50.4 25.1 25.5

As shown in Table 2 above, it was confirmed that the antimicrobial preservative composition prepared according to Example 1 of the present invention was excellent because the spray angle was wider than those of Comparative Examples 1 to 7.

Comparative Example 6 using a large amount of butylene glycol and Comparative Example 7 using the same amount of pentylene glycol and butylene glycol showed that the injection angle was narrow, so the use of butylene glycol and pentylene glycol for the injection angle It was confirmed that the presence and content had an effect.

Accordingly, the antibacterial preservative composition of Example 1 can be used as an antibacterial preservative composition for mist.

A preparation example of a cosmetic composition suitable for applying the present invention will be presented.

Preparation Example 1: Mist

Preparation examples of the mist in the cosmetic containing the antibacterial preservative composition of Example 1 are shown in Table 3 below.

ingredient content (wt%) Antimicrobial Preservative Composition of Example 1 2.0 glycerin 10.0 glycolipid 0.35 polyglycerin 1.5 oil 2.0 squalane 2.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation Example 2: lotion

Examples of preparation of a lotion among cosmetics containing the antibacterial preservative composition of Example 1 are shown in Table 4 below.

ingredient content (wt%) Antimicrobial Preservative Composition of Example 1 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PG 1500 1.0 allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium Hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation Example 3: Lotion

Preparation examples of lotions in cosmetics containing the antibacterial preservative composition of Example 1 are shown in Table 5 below.

ingredient content (wt%) Antimicrobial Preservative Composition of Example 1 3.0 propylene glycol 6.0 glycerin 4.0 triethanolamine 1.2 Tocopheryl Acetate 3.0 liquid paraffin 5.0 squalane 3.0 Macadamina Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation Example 4: Nourishing Cream

Preparation examples of a nutritional cream in a cosmetic comprising the antibacterial preservative composition of Example 1 are shown in Table 6 below.

ingredient content (wt%) Antimicrobial Preservative Composition of Example 1 1.0 lipophilic monostearate glycerin 1.5 cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearylisostearate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation 5: Essence

Preparation examples of the essence in the cosmetic including the antibacterial preservative composition of Example 1 are shown in Table 7 below.

ingredient content (wt%) Antimicrobial Preservative Composition of Example 1 1.5 glycerin 10.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 Sodium Hyaluronate 8.0 Carboxyvinyl Polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation Example 6: Emulsion for mask pack

Preparation examples of the emulsion for a mask pack among cosmetics containing the antibacterial preservative composition of Example 1 are shown in Table 8 below.

ingredient content (wt%) Antimicrobial Preservative Composition of Example 1 1.0 polyvinyl alcohol 15.0 Cellulose Gum 0.15 glycerin 3.0 PEG 1500 2.0 cyclodextrin 0.15 DL-Panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Claims (12)

100 parts by weight of ginseng root extract, 20 to 60 parts by weight of pine leaf extract, and 20 to 60 parts by weight of mugwort leaf extract as active ingredients,
The antibacterial preservative composition, characterized in that the pine leaf extract and mugwort leaf extract are mixed in a weight ratio of 1: 1-3. delete delete The method according to claim 1, wherein the ginseng root extract, pine leaf extract and mugwort leaf extract are each extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, ethylene glycol, ethyl ether, or a mixed solvent thereof. Antibacterial preservative composition. The antibacterial preservative composition according to claim 4, wherein the lower alcohol having 1 to 4 carbon atoms is 20 to 80% methanol, ethanol, butanol or propanol. According to claim 1, wherein the antibacterial preservative composition is Escherichia coli , Klebsiella pneumonia , Pseudomonas aeruginosa , Micrococcus luteus ( Micrococcus luteus ) and Staphylococcus aureus from the group consisting of one or more selected bacteria; And Candida albicans ( Candida albicans ) and black mold ( Aspergillus niger ) Antibacterial preservative composition, characterized in that it has antibacterial activity against one or more fungi selected from the group consisting of. natural extracts including ginseng root extract, pine leaf extract and mugwort leaf extract; And butylene glycol, pentylene glycol, a naturally-derived polyhydric alcohol comprising at least one selected from the group consisting of glycerin and glyceryl caprylate;
The natural extract is contained in 20 to 60 parts by weight of pine leaf extract and 20 to 60 parts by weight of mugwort leaf extract based on 100 parts by weight of ginseng root extract, and the pine leaf extract and mugwort leaf extract are mixed in a weight ratio of 1: 1-3. An antibacterial preservative composition for cosmetics. 8. The method of claim 7, wherein the composition comprises 80 to 150 parts by weight of butylene glycol, 400 to 800 parts by weight of pentylene glycol, 100 to 400 parts by weight of glycerin, and 300 to 300 parts by weight of glyceryl caprylate based on 100 parts by weight of the ginseng root extract. Antibacterial preservative composition for cosmetics, characterized in that it is included in 700 parts by weight. The antibacterial preservative composition for cosmetics according to claim 7, wherein the pentylene glycol and butylene glycol are mixed in a weight ratio of 1: 0.01-0.5. natural extracts including ginseng root extract, pine leaf extract and mugwort leaf extract; And butylene glycol, pentylene glycol, a naturally-derived polyhydric alcohol comprising at least one selected from the group consisting of glycerin and glyceryl caprylate;
The natural extract is contained in 20 to 60 parts by weight of pine leaf extract and 20 to 60 parts by weight of mugwort leaf extract based on 100 parts by weight of ginseng root extract, and the pine leaf extract and mugwort leaf extract are mixed in a weight ratio of 1: 1-3. Antibacterial preservative composition for mist, characterized in that. The antibacterial preservative composition for mist according to claim 10, wherein the spraying angle of the antibacterial preservative composition for mist is 55 to 75°. A cosmetic composition comprising the antibacterial preservative composition of claim 7.

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