KR102283326B1 - Cosmetic compositions containing Fermented products of Lactobacillus, Fermented products of Lactococcus, Sacchromyces peptide, Lactobacillus acidophilus/grape ferment - Google Patents

Abstract

The present invention provides a cosmetic composition comprising liposomes in which active ingredients such as Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, Lactobacillus acidophilus grape fermented product, etc. aim to Lactobacillus fermented product, lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product are encapsulated in liposomes stabilized with vegetable oil and lecithin as active ingredients, and a cosmetic composition containing them is prepared by Increases anti-inflammatory effect.

Description

Cosmetic compositions containing Fermented products of Lactobacillus, Fermented products of Lactococcus, Sacchromyces peptide, Lactobacillus acidophilus /grape ferment}

The present invention relates to a cosmetic composition comprising liposomes in which active ingredients such as Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product are encapsulated. Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product are encapsulated in liposomes as active ingredients to prepare a cosmetic composition containing them, thereby enhancing skin moisturizing power, antioxidant and anti-inflammatory effects. is about

In general, the skin is a part of the body directly exposed to the external environment, and when exposed to excessive ultraviolet rays or pollutants, skin irritation such as erythema, edema, itching, and inflammatory reactions are induced. These skin troubles are not only a cosmetic problem, but also the substances produced during the inflammatory reaction cause pigmentation incidentally, and collagen, elastin, hyaluronic acid, and muco are involved in skin elasticity. It causes skin damage by accelerating the breakdown of mucopolysaccharides.

According to the free radical theory presented by D. Harman in the mid-1950s, free radicals destroy cells, cut the connective tissue of the dermal layer of the skin, or cause cross-linking, making it difficult to maintain moisture in the dermal layer and It causes various problems such as wrinkle formation, skin cancer, cell death, rheumatoid arthritis, atopic dermatitis, and acne.

The causes of free radicals are known as phagocytosis of white blood cells, electron transport system during ATP production in mitochondria, the action of Myeloper Oxide (MPO), ultraviolet rays, tobacco, normal metabolic processes, stress, pollutants, and bacteria. there is.

The phagocytosis refers to an action in which the cell body receives foreign substances or bacteria into its own body and decomposes and detoxifies it.

Of course, the human body has superoxide dismutase (SOD), catalase, vitamin E, vitamin C, and ubiquinol, which are antioxidants (radical scavengers). It is neutralized, and free radicals gradually increase.

The increased free radicals destroy the connective tissue of the dermis such as collagen, elastin, hyaluronic acid, mucopolysacchride, etc. , dermatitis, acne, and skin cancer.

When the skin loses its ability to retain moisture in the epidermal layer structurally, it cannot maintain normal skin moisture and loses both skin elasticity and health. Most of the water our body needs to sustain life takes up 70% of water in the body and 10 to 20% in the stratum corneum of the skin epidermis in normal skin, so the cells and unique proteins that make up the skin are able to function normally. make it possible

On the other hand, when the moisture in the stratum corneum of the epidermis is lowered to less than 10%, the elasticity and flexibility of the skin disappear and the skin becomes dry and rough. This causes skin dryness, which causes inflammation and trouble, and the activity of keratinocytes and fibroblasts decreases.

In addition, the decrease in skin elasticity as the water holding power decreases, the elasticity of collagen and elastin, which supported the pore wall of the skin, decreases, and the power to tighten the pores is weakened and the pores increase. It is very important to maintain the moisture content of the stratum corneum above all else because the skin barrier breaks down due to the increased pores and dryness, and bacteria invade and cause inflammation.

Currently, occlusive agents, which are hydrophobic substances such as petrolatum, lanolin, cocoa butter, mineral oil, jojoba oil, shea butter, and silicone, are used to prevent moisture loss in the skin. About 20 to 50 kinds of general raw materials such as sodium lauryl sulfate, dimethyl sulfoxide, croton oil and sodium hydroxide are used. When the above ingredients are added to the product, not only skin irritation but also immune reactions such as itchiness, allergy, and skin inflammation are accompanied by an increase in the stratum corneum as well as transepidemal water loss, which causes skin inflammation.

Accordingly, the conventional moisturizing agent contained in the cosmetic composition has a limitation in obtaining a skin moisturizing effect, a protective effect, etc. as it is accompanied by a side effect of causing trouble accompanied by irritation and inflammation to the skin.

To supplement this, a component with a structure similar to sebum, a natural moisturizing factor (NMF), a polyol of a trihydric alcohol having three hydroxyl groups (-OH), etc. were added to act as a moisturizer.

However, depending on the geographical or environmental conditions, the effect of temperature and humidity may cause stickiness or discomfort to the feeling of use, and in some cases, a problem of depriving moisture conversely occurred.

In order to solve this problem, we have recently developed natural-derived physiologically active materials from animals and plants to microorganisms and introduced them into cosmetics to maintain and improve the skin's own natural defense function, thereby suppressing moisture loss in the skin and increasing elasticity and moisturizing power. The R&D of cosmetic products is becoming active.

Therefore, in this study, it is essential to develop a bioactive material that suppresses skin irritation, allergy, and inflammation, and a stable cosmetic for moisture retention.

The present invention enhances skin moisture content, suppresses moisture loss in the epidermal layer, does not cause side effects such as skin irritation, allergies and troubles, and is a cosmetic composition with excellent skin moisturizing power, antioxidant activity and anti-inflammatory effect, and greatly improved stability of the formulation is to provide

The above-described object of the present invention is a liposome constituent material containing one or more emulsifiers and solvents, and Lactobacillus fermented products, Lactococcus fermented products, yeast polypeptides, and Lactobacillus acidophilus grape fermented products as an encapsulation material. It is achieved by providing a cosmetic composition having a skin moisturizing effect, antioxidant and anti-inflammatory effect.

According to a preferred feature of the present invention, 90.0 to 99.8 wt% of the Lactobacillus ferment, 0.01 to 1.0 wt% of the Lactococcus ferment, 0.01 to 1.0 wt% of the yeast polypeptide, and the Lactobacillus acidophilus grape fermentation 0.01 to 2.0% by weight, and 0.01 to 2.0% by weight of the lecithin.

According to a preferred feature of the present invention, the formulation of the composition is one of an emulsified sunscreen cream, an emulsified foundation, an emulsified makeup base, an oil cake-type foundation, a two-way cake, or a powder pact.

The cosmetic composition comprising, as an active ingredient, liposomes in which Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product according to the present invention are stabilized, inhibits skin moisturizing power and moisture loss in the epidermal layer, , conventionally used moisturizing ingredients are composed of chemicals and have disadvantages that can cause side effects such as skin irritation, allergies, toxicity, and troubles. Dophilus grape fermented product is a naturally-derived physiologically active substance that does not cause side effects such as skin allergies, troubles, and toxicity. It can be usefully used as a skin moisturizer and functional cosmetic for improving the reaction, or a cosmetic and therapeutic agent for improving atopic diseases.

1 is a graph showing the cell proliferation rate of Examples 1 to 4 and Comparative Examples 1 to 3;
Figure 2 is a graph showing the skin moisturizing effect of Example 5 and Comparative Example 4.
Figure 3 is a graph showing the effect of improving the transdermal water loss of Example 5 and Comparative Example 4.
4 is a graph showing the free radical scavenging effect of Examples 1 to 4 and Comparative Examples 1 to 3;
5 is a graph showing the lipid peroxidation inhibitory effect of Examples 1 to 4 and Comparative Examples 1 to 3;
6 is a graph showing the lipoxygenase activity inhibitory effect of Examples 1 to 4 and Comparative Examples 1 to 3;

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown by way of example of the embodiment. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, certain shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the present invention, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Hereinafter, the present invention will be described in detail including specific examples.

The cosmetic composition comprising, as an active ingredient, a Lactobacillus fermented product, a lactococcus fermented product, a yeast polypeptide, and a Lactobacillus acidophilus grape fermented product according to the present invention is a Lactobacillus fermented product, a lactococcus fermented product, and a yeast polypeptide. , Lactobacillus acidophilus grape fermentation was stabilized with liposomes containing at least one surfactant and vegetable oil to maximize skin moisturizing, skin antioxidant and anti-inflammatory effects.

The fermented Lactobacillus refers to a product obtained by culturing and fermenting Lactobacillus in a culture medium, sterilizing it with a sterilizer, and filtering impurities with a filter having a size of 0.1 to 0.3 μm.

The fermented lactococcus refers to a product obtained by culturing and fermenting lactococcus in a culture medium, sterilizing it with a sterilizer, and filtering impurities with a filter having a size of 0.1 to 0.3 μm.

The yeast polypeptide refers to a separation of peptides from a filtrate obtained by filtration of a fermented strain, and more specifically, a separation of peptides from a filtrate obtained by filtration of a fermented Saccharomyces.

The Lactobacillus acidophilus grape fermented product is obtained by extracting grapes at a ratio of 1:40 at a temperature of 50 to 70° C. for 4 to 8 hours and inoculating the filtered grape extract with Lactobacillus acidophilus to 2%. It refers to a fermented product fermented for 40 to 56 hours at a temperature of 35 to 39°C.

The liposome is prepared by a mixture containing a surfactant, vegetable oil, and fermented product, and preferably does not contain a monohydric alcohol.

The surfactant is preferably any one or more of lecithin, lauric acid, lauric glucoside, decyl glucoside, cocoglucoside, sodium cocoyl apple amino acid, sodium cocoyl glutamate, and potassium cocoyl glycinate, most preferably It would be appropriate to use lecithin.

The vegetable oil means an oil extracted from a plant, and vegetable oils that can be used therein include camellia oil (Camellia japonica seed oil), oleifera oil (Camellia oleifera seed oil), coconut oil (Cocos nucifera oil), castor oil (Ricinus) communis seed oil), canola oil (Canola oil), olive oil (Olea europaea fruit oil) and at least one vegetable oil selected from the group consisting of palm oil (Elaeis guineensis oil) will be preferred.

The liposome will preferably have a form in which Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product are collected inside the spherical liposome.

The cosmetic composition according to an embodiment of the present invention contains a functional ingredient in the form of a liposome in which Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, Lactobacillus acidophilus grape fermented product, vegetable oil and lecithin are mixed. do.

If the content of vegetable oil and lecithin in the cosmetic composition according to an embodiment of the present invention is less than 0.05% by weight, the main effect of the composition is deteriorated, and if it is 5% by weight or more, it is difficult to use as a cosmetic, such as the feeling of use of the prepared formulation decreases. It is preferable to add each in an amount of 0.05 to 5% by weight.

In addition, in the case of Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product in the cosmetic composition, 90 to 99.6 wt% of Lactobacillus fermented product, Lactococcus, as a fermented product with functional ingredients It is preferable to add in an amount of 0.01 to 1.0% by weight of fermented product, 0.01 to 1.0% by weight of yeast polypeptide, and 0.01 to 1.0% by weight of fermentation of Lactobacillus acidophilus grapes.

The cosmetic composition according to an embodiment of the present invention contains an active ingredient in the form of a liposome in which Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, Lactobacillus acidophilus grape fermented product, vegetable oil and lecithin are mixed. do.

The ingredients included in the cosmetic composition of the present invention are conventional in the cosmetic composition in addition to the above-mentioned Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, Lactobacillus acidophilus grape fermented product and liposome stabilized with vegetable oil and lecithin. It includes components used as a pharmaceutical composition, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments, and conventional adjuvants such as pigments and fragrances, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, a pack, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder. When the formulation of the present invention is a paste, cream or gel, any one or more of animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as a carrier component can be used by selecting .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butyl Renglycol, 1,3-butyl glycol oil, polyoxyethylene hydrogenated castor oil, glycerol, glycerin, aliphatic ester, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleate, paraffin, Sorbitan stearate, lipophilic monostearic acid glycerin, stearic acid, glyceryl stearate/PEG-400 stearate, carboxypolymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, stearate-4, dicetylphosphate , macadamia oil, carboxyvinyl polymer, xanthan gum, or fatty acid ester of sorbitan may be selected and used.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol, glycerin, butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Suspending agents such as microcrystalline cellulose, hydroxyethyl cellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate , sorbitan sesquioleate, glyceryl monostearate/glyceryl stearate/polyoxyethylene stearate, wax, paraffin, squalane, caprylic/capric triglyceride, carboxyvinyl polymer, triethanolamine, agar or triglyceride Any one or more of Kant may be selected and used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Any one or more of ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be selected and used.

In addition, the use efficacy of the cosmetic composition according to an embodiment of the present invention can be appropriately adjusted according to an individual's skin condition and condition, and external environmental conditions.

Hereinafter, preferred examples and experimental examples are presented to help the understanding of the present invention. However, the following Examples and Experimental Examples are only provided for easier understanding of the present invention, and the technical level and scope of the present invention are not limited thereby.

< Example 1-1 to 1-4 and comparative example 1-1 to 1-3 > Preparation of active ingredient composition with skin moisturizing effect

Fermented Lactobacillus and Lactococcus were cultured in an appropriate culture medium, fermented, sterilized with a sterilizer, and obtained by filtering impurities with a 0.2 μm filter. Yeast polypeptide was obtained by filtering Saccharomyces ferment Peptides were separated from the obtained filtrate, and grapes were extracted at a ratio of 1:40 at 60°C for 6 hours, and Lactobacillus acidophilus was inoculated to 2% of the filtered grape extract at 37°C for 48 hours. It was fermented for a period of time to obtain a fermentation product of Lactobacillus acidophilus grapes. Composition (Example) containing liposomes in which Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, Lactobacillus acidophilus grape fermentation product of the present invention are stabilized (Example) Comparative Examples 1-1 to 1-3 and Examples 1-1 to 1-4 were prepared by mixing Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product by mixing ratio. Table 1 shows.

sample
Raw material name note Lactobacillus fermented product Lactococcus Fermented Product yeast polypeptide Lactobacillus acidophilus grape fermentation vegetable oil lecithin One - - - - 0.05 0.05 Comparative Example 1-1 2 99.7 0.1 0.1 0.1 - - Comparative Example 1-2 3 98.5 0.5 0.5 0.5 - - Comparative Example 1-3 4 98.4 0.5 0.5 0.5 0.05 0.05 Example 1-1 5 99.6 0.1 0.1 0.1 0.05 0.05 Example 1-2 6 98.3 0.5 0.5 0.5 0.1 0.1 Examples 1-3 7 99.68 0.1 0.1 0.1 0.01 0.01 Examples 1-4

< Example 2 and comparative example 2> With skin moisturizing including the composition of the present invention cosmetics Preparation of the composition

According to the cosmetic composition ratio of Table 2 below, Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product were stabilized with vegetable oil and lecithin in Example 1-2 containing liposomes. Cosmetic composition of Comparative Example 2 containing the cosmetic of Example 2 and Comparative Example 1-2, which did not liposome the Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product was prepared.

Ingredient name Sample name Example 1-2 Comparative Example 1-2 A liposome
(Lactobacillus fermented product / Lactococcus fermented product / yeast polypeptide / Lactobacillus acidophilus grape fermented product) 5.0 - liposome
(Lactobacillus fermented product / Lactococcus fermented product / yeast polypeptide / Lactobacillus acidophilus grape fermented product - 5.0 B Methylglucose sesquistearate 3.0 3.0 Glyceryl Stearate 0.8 0.8 Polysorbate 60 1.5 1.5 cetyl alcohol 1.5 1.5 beeswax 0.5 0.5 Caprylic/Capric Triglycerides 5.0 5.0 shea butter 0.7 0.7 dimethicone 2.0 2.0 Tocopheryl Acetate 0.3 0.3 fragrance, preservative a very small amount a very small amount
note
Example 2
Comparative Example 2

<Test Example 1> Evaluation of cell nontoxicity and cell proliferation effect

The cell proliferation effect using cell culture was measured for the samples prepared in Comparative Examples 1-1 to 1-3 and Examples 1-1 to 1-4.

1) Experimental method

Cytotoxicity and proliferation experiments were performed in the following way. Cells being cultured (fibroblast, 3T3 cells) were dispensed in a 96-well microplate at 5,000 cells/well and incubated in a thermostat for 30 minutes. It was administered and cultured for 72 hours. Then, thiazoline blue was administered and further incubated for 4 hours. After discarding all of the culture medium, a reaction stop solution was added to each well of the microplate, stirred for 5 minutes, and absorbance was measured at 570 nm. The control group was co-cultured under optimal conditions for cell growth by administering 10% Fetal Bovine Serum (FBS) medium as much as the sample injection amount. .

2) Experiment result

The cell proliferation effect was calculated by Equation 1, and the results are shown in Table 3 below.

sample

Concentration (v/v%) Cell proliferation effect (%) comparative example
1-1 comparative example
1-2 comparative example
1-3 Example
1-1 Example
1-2 Example
1-3 Example
1-4 0 (control) 100.0 100.0 100.0 100.0 100.0 100.0 100.0 0.1 100.1 101.6 101.2 104.5 104.1 102.3 103.2 0.5 100.3 102.1 101.8 106.9 108.7 103.3 104.9 1.0 100.6 103.3 102.5 110.2 112.4 105.5 106.8 5.0 100.7 104.6 103.4 113.4 116.5 107.8 109.1 10.0 100.9 105.2 104.1 115.1 119.9 109.2 112.3

This Test Example 1 was an experiment to confirm the cytotoxicity and proliferation of liposomes stabilized with Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product with vegetable oil and lecithin. As shown in Table 3, when the cell proliferation under the optimal cell growth conditions was 100%, it was confirmed that Comparative Examples 1-1 to 1-3 and Examples 1-1 to 1-4 all had a cell proliferation effect. .

In addition, the cell proliferation effect means that liposomes stabilized with Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product with vegetable oil and lecithin are not toxic to cells. As a result, from the cell proliferation effect experiment, liposomes stabilized with vegetable oil and lecithin in the Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product of the present invention with excellent cell proliferation effect and It was confirmed that it was safe without cytotoxicity.

Among them, in the case of Example 1-2, it was confirmed that there was an excellent cell proliferation effect compared to Examples 1-1, 1-3, and 1-4.

< test example 2 > Skin moisturizing effect

Clinical moisturizing effect and durability were measured for the cosmetic compositions of Examples 1-2 and Comparative Examples 1-2.

1) Experimental method

For 20 test subjects (males and females aged 20-45 years), twice a day for 4 weeks, each of the creams of Example 2 and Comparative Example 2 were applied on both sides of the face before application, after 2 weeks, and The improvement effect was measured by quantifying the skin moisture state after 4 weeks by using a Corneometer CM 820 (Corage + Khazaka, Germany) as an electrical conductivity according to the amount of moisture in the epidermis.

2) Experiment result

The experimental results for skin moisturizing improvement are shown in Table 4 below.

Sample name skin moisturizing effect before application after 2 weeks after 4 weeks Example 2 24.9 32.7 38.4 Comparative Example 2 25.2 25.7 26.1

As shown in Table 4, as a result of comparing the moisturizing effect of Example 1-2 and Comparative Example 1-2, Lactobacillus fermented / Lactococcus fermented / Yeast polypeptide / Lactobacillus ash than cosmetics containing simple liposome form. In Example 1-2 containing Dophilus grape fermented product, it was confirmed that the skin moisturizing effect was improved as time passed.

<Test Example 3> Evaluation of transdermal water loss

The amount of transdermal moisture loss was measured for the cosmetic compositions of Examples 1-2 and Comparative Examples 1-2.

1) Experimental method

^{A certain amount (2mg/cm 2} ) of the cream of Examples 1-2 and Comparative Example 1-2 was applied to 20 test subjects (male and female aged 20-45 years), respectively, in 6 sites, the test site of the left forearm. After applying the cream, it was rubbed well, and the amount of transdermal water loss was measured using TEWAMETER TM210 (C+K eletronic GmhH. Germany). Short-term moisturizing effect was evaluated by measuring the moisture increase rate (%) 30 minutes after application, 2 hours, 4 hours, and 6 hours after application, based on the water retention amount of 0 hours before application. During the measurement, the amount of transdermal moisture loss was measured three times consecutively while keeping the probe horizontal for 60 seconds by touching the probe vertically.

2) Experiment result

The improvement effect was calculated by Equation 2, and the results are shown in Table 5 below.

Sample name Water loss (%) 0 hours 30 minutes later after 2 hours after 4 hours after 6 hours Example 2 0.0 33.11 31.06 25.43 16.55 Comparative Example 2 0.0 15.49 2.97 0.33 0.0

As shown in Table 5, as a result of comparing the amount of transdermal moisture loss in Example 1-2 and Comparative Example 1-2, it was confirmed that Example 1-2 had a significantly higher moisture improvement rate than Comparative Example 1-2. In particular, when 30 minutes had elapsed after application, in Example 1-2, the improvement rate was increased by about 33% compared to before application.

On the other hand, in Comparative Example 1-2, the improvement rate of 15.49% was increased for 30 minutes after application, but the improvement rate decreased to 2.97% after 2 hours, and returned to the original skin moisture content after 4 hours.

<Test Example 4> Free radical scavenging effect evaluation

The free radical scavenging effect was measured for the samples prepared in Comparative Examples 1-1 to 1-3 and Examples 1-1 to 1-4.

1) Experimental method

Experiments were performed using the DPPH (1,1-diphenyl-2 picryl-hydrazyl) method (see: Blois. MSNature 181, 1190, 1958), and DPPH and Quercetin were used by Sigma. . Samples of Comparative Examples 1-1 to 1-3 and Examples 1-1 to 1-4 of various concentrations (0.1, 0.5, 1, 5, 10 v/v%) in 0.2 mM DPPH methanol solution were mixed with ethanol or methanol solution 2ml is added, stirred well, and then reacted at room temperature for 10 minutes. And absorbance was measured at 517 nm, and purified water was used instead of each sample as a blank test.

2) Experiment result

The free radical scavenging effect was obtained using Equation 3 below, and the results are shown in Table 6 below.

sample

Concentration (v/v%) Free radical scavenging effect (%) comparative example
1-1 comparative example
1-2 comparative example
1-3 Example
1-1 Example
1-2 Example
1-3 Example
1-4 0 (control) 0 0 0 0 0 0 0 0.1 1.2 11.2 10.5 17.0 19.1 11.7 13.1 0.5 2.5 23.7 22.8 29.2 33.0 24.2 26.3 1.0 3.3 38.7 37.4 55.3 58.8 40.8 45.4 5.0 4.9 74.1 72.9 85.1 86.9 76.8 78.3 10.0 6.8 92.8 90.4 97.4 98.1 93.6 94.5

As shown in Table 6, it was confirmed that the free radical scavenging effect was excellent in all samples except Comparative Example 1-1. As with the samples of Comparative Examples 1-2 and 1-3, excellent antioxidant effects were also confirmed in samples composed only of a mixture of Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product, It was confirmed that the free radical scavenging effect of Example 1-2 was the most excellent among the samples of Examples 1-1 to 1-4 in the form of liposomes.

<Test Example 5> Lipid peroxydation inhibitory effect evaluation

Lipid peroxydation inhibitory effect was measured for the samples prepared in Comparative Examples 1-1 to 1-3 and Examples 1-1 to 1-4.

1) Experimental method

Dissolve linolenic acid to 0.1% in 8% sodium lauryl sulfate aqueous solution, take 3.9 ml, add 0.1 ml of samples according to concentration (0.1, 0.5, 1, 5, 10 v/v%), and then irradiate with UV rays. Investigate for an hour Then, take 1 ml, add 1.5 ml of 0.8% TBA aqueous solution and 1.5 ml of 20% acetic acid (pH 3.5), cool in a bath for 1 hour, add 1 ml of purified water and 5 ml of n-BuOH:Pyridine (15:1), shake, and centrifuge. After separation, take the n-BuOH layer and measure the absorbance at 532 nm.

2) Experiment result

In the blank test, no sample was added. The effect of lipid peroxidation was calculated using Equation 4 below, and the results are shown in Table 7 below.

sample

Concentration (v/v%) Lipid peroxidation inhibitory effect (%) comparative example
1-1 comparative example
1-2 comparative example
1-3 Example
1-1 Example
1-2 Example
1-3 Example
1-4 0 (control) 0 0 0 0 0 0 0 0.1 0.15 3.5 3.2 5.1 5.9 4.0 4.3 0.5 0.15 11.2 10.5 15.3 16.2 13.1 13.7 1.0 0.22 29.5 28.3 36.8 37.9 31.8 34.4 5.0 0.25 63.1 62.6 71.0 72.3 67.6 69.9 10.0 0.31 74.8 74.1 87.3 89.8 79.9 82.2

As shown in Table 7, it was confirmed that the lipid peroxidation inhibition rate was excellent in all samples except Comparative Example 1-1. As with the samples of Comparative Examples 1-2 and 1-3, excellent lipid peroxidation inhibition rates were confirmed in samples composed only of a mixture of Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product. , It was confirmed that the lipid peroxidation inhibition rate of Example 1-2 among Examples 1-1 to 1-4 in the form of liposomes was the best.

<Test Example 6> Lipoxygenase (Lpoxygenase) activity inhibitory effect evaluation

The lipoxygenase activity inhibitory effect was measured for the samples prepared in Comparative Examples 1-1 to 1-3 and Examples 1-1 to 1-4.

1) Experimental method

The experiment was performed using the TBAS method, and those of Sigma (SIGMA) were used for Linoleic acid, Lipoxygenase, Thiobabitulic acid, and Quercetin used in the experiment. To 1 ml of 1 mM Linoleic acid, 0.05 ml of Comparative Examples 1-1 to 1-3 and Examples 1-1 to 1-4 of various concentrations (0.1, 0.5, 1, 5, 10 v/v%) were added, and lipoxygenase 0.95 After administration of ml, the mixture is stirred well and allowed to react at 25°C for 10 minutes. Then, 0.5 ml of trichloroacetic acid was administered, 1 ml of thiobarbiturlic acid was added, and after heating for 10 minutes, the reaction was terminated by cooling in ice water for 2 to 3 minutes. After completion of the reaction, 2 ml of Butanol was added to the reaction solution, centrifuged at 4,000 x g for 5 minutes, and absorbance was measured at 535 nm. In this case, purified water was used instead of each sample as a blank test.

2) Experiment result

The lipoxygenase activity inhibitory effect was obtained using Equation 5 below, and the results are shown in Table 8 below.

sample

Concentration (v/v%) Lipoxygenase activity inhibitory effect (%) comparative example
1-1 comparative example
1-2 comparative example
1-3 Example
1-1 Example
1-2 Example
1-3 Example
1-4 0 (control) 0 0 0 0 0 0 0 0.1 0.09 1.9 1.2 3.9 4.3 2.5 2.8 0.5 0.13 3.7 3.1 7.8 8.4 5.9 6.7 1.0 0.19 11.6 10.8 20.4 22.9 16.3 17.6 5.0 0.24 43.8 42.9 60.1 63.3 54.2 55.4 10.0 0.26 64.8 63.7 84.3 88.7 77.4 79.1

As shown in Table 8, it was confirmed that the lipoxygenase activity inhibition rate was excellent in all samples except Comparative Example 1-1. As with the samples of Comparative Examples 1-2 and 1-3, excellent inhibition was confirmed even in a sample composed only of a mixture of Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product, and liposomes Of the forms of Examples 1-1 to 1-4, it was confirmed that the lipoxygenase activity inhibition rate of Example 1-2 was the most excellent.

Therefore, the Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product are encapsulated as active ingredients in the liposome stabilized with vegetable oil and lecithin of the present invention. , it can be seen that it is useful as a cosmetic composition due to its excellent antioxidant and anti-inflammatory effects.

Hereinafter, based on the results of the above experimental examples, Lactobacillus fermented products, Lactococcus fermented products, yeast polypeptides, and Lactobacillus acidophilus grape fermented products were encapsulated as active ingredients in liposomes stabilized with vegetable oil and lecithin. A cosmetic composition containing the mixture is formulated and presented as follows. However, the composition of the present invention is not limited to the following formulation examples.

< Formulation Example 1> In liposomes stabilized with vegetable oil and lecithin having skin moisturizing, antioxidant, and anti-inflammatory effects according to the present invention Lactobacillus ferment , Lactococcus ferment , Yeast polypeptide , Lactobacillus acidophilus Preparation of lotion containing fermented grapes

Table 9 shows the formulation examples of lotion (skin lotion) among cosmetics containing liposomes stabilized with lactobacillus fermented product / lactococcus fermented product / yeast polypeptide / lactobacillus acidophilus grape fermented product with vegetable oil and lecithin same.

number Raw material content (wt%) One Liposome (Lactobacillus ferment / Lactococcus ferment / yeast polypeptide
/Lactobacillus acidophilus grape fermentation) 5.0
2 glycerin 3.0 3 butylene glycol 2.0 4 propylene glycol 2.0 5 Polyoxyethylene hydrogenated castor oil 1.0 6 ethanol 10.0 7 triethanolamine 0.1 8 antiseptic a very small amount 9 pigment a very small amount 10 Spices a very small amount 11 Purified water a very small amount

<Production method>

After putting No. 2, 3, 4, and 8 into No. 11 in order and stirring to dissolve, No. 5 is heated to about 60° C. to dissolve, No. 10 is put in to dissolve, and then No. 11 is added. Finally, after adding Nos. 6, 7, and 9, add 1, stir sufficiently, and then ripen.

< Formulation Example 2> In liposomes stabilized with vegetable oil and lecithin having skin moisturizing, antioxidant, and anti-inflammatory effects according to the present invention Lactobacillus ferment , Lactococcus ferment , Yeast polypeptide, Lactobacillus acidophilus Preparation of nutritional lotion containing fermented grapes

Table 10 shows a prescription example of a nutritional lotion among cosmetics containing liposomes in which Lactobacillus fermented product / Lactococcus fermented product / yeast polypeptide / Lactobacillus acidophilus grape fermented product is stabilized with vegetable oil and lecithin.

number Raw material content (wt%) One Liposome (Lactobacillus ferment / Lactococcus ferment / yeast polypeptide
/Lactobacillus acidophilus grape fermentation) 5.0 2 beeswax 1.0 3 Polysorbate 60 1.5 4 Sorbitan Sesquiolate 0.5 5 liquid paraffin 10.0 6 Sorbitan Stearate 1.0 7 lipophilic monostearate glycerin 0.5 8 stearic acid 1.5 9 Glyceryl Stearate/PEG-400 Stearate 1.0 10 propylene glycol 3.0 11 carboxy polymer 0.1 12 triethanolamine 0.2 13 antiseptic a very small amount 14 pigment a very small amount 15 Spices a very small amount 16 Purified water remaining amount

<Production method>

10, 11, 13, 16 are mixed and stirred at 80 ~ 85 ℃ and heated to 80 ~ 85 ℃, put into the manufacturing unit, and then the emulsifier works, and 2, 3, 4, 5, 6, 7, 8, 9, 12 is heated to 80 ~ 85 ℃ Emulsify after dissolving by heating. After the emulsification is finished, use a stirrer to cool to 50°C, add No. 15, cool to 45°C, add No. 14, put No. 1 at 35°C, cool to 25°C, and then ripen.

< Formulation Example 3> In liposomes stabilized with vegetable oil and lecithin having skin moisturizing, antioxidant, and anti-inflammatory effects according to the present invention Lactobacillus ferment , Lactococcus ferment , Yeast polypeptide, Lactobacillus acidophilus Preparation of nutritional cream containing fermented grapes

Table 11 shows a prescription example of a nutritional cream among cosmetics containing liposomes in which Lactobacillus fermented product / Lactococcus fermented product / yeast polypeptide / Lactobacillus acidophilus grape fermented product is stabilized with vegetable oil and lecithin.

number Raw material content (wt%) One Liposome (Lactobacillus ferment / Lactococcus ferment / yeast polypeptide
/Lactobacillus acidophilus grape fermentation) 5.0 2 stearic acid 2.0 3 cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl Monostearate/Glyceryl Stearate/Polyoxyethylene Stearate 1.0 8 wax 1.0 9 liquid paraffin 4.0 10 squalane 4.0 11 Caprylic/Capric Triglycerides 4.0 12 Carboxyvinyl Polymer 0.3 13 butylene glycol 5.0 14 glycerin 3.0 15 triethanolamine 0.5 16 Purified water remaining amount

<Production method>

12, 13, 14, and 16 are mixed and stirred, heated to 80 ~ 85 ℃, put into the manufacturing unit, and then the emulsifier works, and 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 are heated to 80 ~ After dissolution by heating to between 85 °C, 15 is added and stirred to put into the manufacturing unit and emulsify. When the emulsification is finished, it is cooled to 35°C while stirring using a stirrer, and then cooled to 25°C by adding No. 1 and then aged.

< Formulation Example 4> In liposomes stabilized with vegetable oil and lecithin having skin moisturizing, antioxidant, and anti-inflammatory effects according to the present invention Lactobacillus ferment , Lactococcus ferment , Yeast polypeptide, Lactobacillus acidophilus Preparation of Essence Containing Grape Fermentation

Table 12 shows the formulation examples of essences in cosmetics containing liposomes stabilized by lactobacillus fermented product/lactococcus fermented product/yeast polypeptide/lactobacillus acidophilus grape fermented product with vegetable oil and lecithin.

number Raw material content (wt%) content (wt%) One Liposome (Lactobacillus ferment / Lactococcus ferment / yeast polypeptide
/Lactobacillus acidophilus grape fermentation) 5.0 - 2 sitosterol 1.7 1.7 3 Polyglyceryl 2-oleate 1.5 1.5 4 ceramide 0.7 0.7 5 Steares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 dicetyl phosphate 0.4 0.4 8 concentrated glycerin 5.0 5.0 9 macadamia oil 15.0 15.0 10 Carboxyvinyl Polymer 0.2 0.2 11 Xanthan Gum 0.2 0.2 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water remaining amount remaining amount

<Production method>

2, 3, 4, 5 and 6 are homogenized at a constant temperature and are referred to as nonionic amphiphilic lipids. After mixing the nonionic amphiphilic lipids 7, 8, and 14, add No. 1 to mix, homogenize at a constant temperature, pass through a microfluidizer, and then slowly add 9 at a constant temperature to homogenize and then again Pass it through a microfluidizer again, and add 10, 11, 12, and 13 to disperse, stabilize and mature.

Although the present invention has been described with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Therefore, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent to that by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality for more clearly explaining the configuration.

Claims (8)

Liposome constituent material containing at least one surfactant and vegetable oil, and
Forming liposomes by containing Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, and Lactobacillus acidophilus grape fermented product as an encapsulation material,
The Lactobacillus fermented product, Lactococcus fermented product, yeast polypeptide, Lactobacillus acidophilus grape fermented product, surfactant and vegetable oil are in a weight ratio of 99.6: 0.1: 0.1: 0.1: 0.05: 0.05 to provide a skin moisturizing effect, A cosmetic composition comprising, as an active ingredient, a lactobacillus fermented product, a lactococcus fermented product, a yeast polypeptide, and a lactobacillus acidophilus grape fermented product, characterized in that it has a skin antioxidant effect and an anti-inflammatory effect on the skin.
According to claim 1,
The liposome is prepared by a mixture containing a surfactant, vegetable oil and fermented product, and Lactobacillus fermented product, lactococcus fermented product, yeast polypeptide, Lactobacillus acido, characterized in that it does not contain a monohydric alcohol A cosmetic composition comprising fermented phyllus grapes as an active ingredient.
delete delete delete According to claim 1,
The surfactant is Lactobacillus fermented product, lactococcus fermented product, yeast polypeptide, Lactobacillus acidophilus grape fermented product as an active ingredient, characterized in that it contains lecithin.
According to claim 1,
The cosmetic composition is in the form of a lotion, skin, lotion, cream, foundation, essence, gel, pack, emulsified sunscreen cream, emulsified foundation, emulsified makeup base, oil cake-type foundation, two-way cake, or powder pact. A cosmetic composition comprising, as an active ingredient, a Lactobacillus fermented product, a lactococcus fermented product, a yeast polypeptide, and a Lactobacillus acidophilus grape fermented product, characterized in that it is formulated.
8. The method of claim 7,
Lactobacillus fermented product, lactobacilli, characterized in that the liposome-stabilized Lactobacillus ferment, Lactococcus ferment, yeast polypeptide, and Lactobacillus acidophilus grape fermented product are contained in an amount of 0.01 to 10% by weight based on the total weight of the composition. A cosmetic composition comprising, as an active ingredient, a couscous fermented product, a yeast polypeptide, and a Lactobacillus acidophilus grape fermented product.

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