KR102208963B1 - 신규한 아크릴산 생성 경로를 갖는 미생물 및 이를 이용한 아크릴산 생산 방법 - Google Patents
신규한 아크릴산 생성 경로를 갖는 미생물 및 이를 이용한 아크릴산 생산 방법 Download PDFInfo
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Abstract
3-HP로부터 3-HP-CoA 및 AA-CoA를 거쳐, AA를 생성하는 경로의 활성이 증가된, 아크릴산 생산능을 갖는 미생물 및 그를 이용하여 아크릴산을 생산하는 방법을 제공한다.
Description
신규한 아크릴산 생성 경로를 갖는 미생물 및 이를 이용한 아크릴산 생산 방법에 관한 것이다.
최근 원유가격의 급등에 의한 불안정성과 탄소배출저감이 글로벌 이슈화됨에 따라 기존 석유를 원료로 하여 화학 공정을 거쳐 생산하던 연료나 화학물질들을 탄소중립적인 생물학적 공정으로 대체하여 생산하려는 노력들이 지속되고 있다.
아크릴산은 10조 원 이상의 시장을 지닌 벌크 화합물 (bulk chemical)이다. 최근에는 친환경적 생산 방법에 대한 요구로 석유계 이외의 경로를 통한 아크릴산 생산 방법의 필요성이 커지고 있다.
비석유계 아크릴산 생산 경로로는, 글리세롤 또는 포도당으로부터 3-히드록시프로피온산 (3-HP)를 생산하고, 이를 화학적으로 분리 및 정제하는 방법이 있을 수 있다. 그러나, 이 방법은 생산된 3-HP를 배양물로부터 분리하고, 정제한 후 촉매를 사용하여 화학적으로 전환하는 단계를 포함한다. 따라서, 3-HP 생산 비용에 대하여 분리, 정제 및 전환 비용이 부가되어, 석유계 화합물 유래 아크릴산 생산법에 경쟁력이 높지 않을 수 있다.
종래의 기술에 의하더라도, 아크릴산 생산능을 갖는 대안적 미생물 및 이를 이용한 아크릴산 생산방법이 요구된다.
일 양상은 유전적으로 조작되지 않은 세포에 비하여 3-히드록시프로피온산 (3-HP)을 3-히드록시프로피오닐-CoA (3-HP-CoA)로 전환하는 것을 촉매하는 CoA transferase, 3-HP-CoA를 아크릴릴-CoA (acrylyl-CoA)로 전환하는 것을 촉매하는 3-HP-CoA 데히드라타제, 및 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소의 활성이 증가되어 있는, 아크릴레이트 생산능을 갖는 미생물을 제공하는 것이다.
다른 양상은 상기 미생물을 배지 중에서 배양하는 단계;를 포함하는, 아크릴레이트를 생산하는 방법을 제공한다.
본 명세서에서 사용된 용어 효소 또는 폴리펩티드 또는 단백질의 "활성 증가" 또는 "증가된 활성"은 효소 또는 폴리펩티드 또는 단백질이 활성을 나타낼 수 있도록 충분한 정도로 증가된 것일 수 있으며, 세포 또는 단리된 폴리펩티드가 비교 가능한 동일 종의 세포 또는 그의 본래 폴리펩티드에서 측정된 활성 수준과 비교하여 높은 활성 수준을 나타냄을 의미한다. 즉 해당 폴리펩티드의 활성이 본래 조작되지 않은 폴리펩티드에 의한 동일한 생화학적 활성보다 약 5% 이상, 약 10% 이상, 약 15% 이상, 약 20% 이상, 약 30% 이상, 약 50% 이상, 약 60% 이상, 약 70% 이상, 또는 약 100% 이상 증가된 것일 수 있다. 증가된 활성을 갖는 폴리펩티드는 당업계에 공지된 임의의 방법을 사용하여 확인될 수 있다.
폴리펩티드의 활성 증가는 폴리펩티드의 발현증가 또는 비활성 (specific activity)의 증가에 의하여 얻을 수 있다. 상기 발현 증가는 폴리펩티드를 코딩하는 폴리뉴클레오티드가 세포에 도입되거나 세포 내 카피 수가 증가되거나, 또는 상기 폴리뉴클레오티드의 조절 영역의 변이에 의한 것일 수 있다. 외부에서 도입되거나 또는 카피 수가 증가되는 폴리뉴클레오티드는 내인성 (endogenous) 또는 외인성 (exogenous)일 수 있다. 상기 내인성 유전자는 미생물 내부에 포함된 유전물질 상에 존재하던 유전자를 말한다. 외인성 유전자는 숙주 세포 게놈으로 도입 (integration)되는 등의 숙주 세포 내로 유전자가 도입되는 것을 의미하며, 도입되는 유전자는 도입되는 숙주세포에 대해 동종 (homologous) 또는 이종 (heterologous)일 수 있다.
용어 "카피 수 증가 (copy number increase)"는 상기 유전자의 도입 또는 증폭에 의한 것일 수 있으며, 조작되지 않은 세포에 존재하지 않는 유전자를 유전적 조작에 의해 갖게 되는 경우도 포함한다. 상기 유전자의 도입은 벡터와 같은 비히클을 매개하여 이루어질 수 있다. 상기 도입은 상기 유전자가 게놈에 통합되지 않은 임시적 (transient) 도입이거나 게놈에 삽입되는 것일 수 있다. 상기 도입은 예를 들면, 목적하는 폴리펩티드를 코딩하는 폴리뉴클레오티드가 삽입된 벡터를 상기 세포로 도입한 후, 상기 벡터가 세포 내에서 복제되거나 상기 폴리뉴클레오티드가 게놈으로 통합됨으로써 이루어질 수 있다.
용어 "유전자 (gene)"는 특정 단백질을 발현하는 핵산 단편을 의미하며, 코딩영역 또는 코딩영역 외 5'-비코딩 서열 (5'-non coding 서열)과 3'-비코딩 서열 (3'-non coding 서열) 등의 조절 (regulatory) 서열을 포함할 수 있다. 상기 조절 영역은 프로모터, 인핸서, 오퍼레이터, 리보좀 결합 부위, polyA 결합 서열, 터미네이터 영역 등을 포함할 수 있다.
"이종성 (heterologous)"은 천연 (native)이 아닌 외인성 (foreign)을 의미한다.
"분비 (secretion)"는 물질이 세포 내부에서 주변세포질공간 (periplasmic space)이나 세포 외 환경으로 이동되는 것을 의미한다.
"세포 (cell)", "균주 (strain)", 또는 "미생물 (microorganism)"은 교체 사용이 가능한 것으로서, 박테리아, 효모, 곰팡이 등을 포함한다.
"아크릴산 (acrylic acid)"은 아크릴산 또는 아크릴레이트, 또는 그 염을 포함하며 이들 서로 교환가능하게 사용될 수 있다. 아크릴산은 미생물의 발효 또는 효소 반응에 의해 생산될 수 있다.
효소 또는 폴리펩티드의 "활성의 감소" 또는 "감소된 활성"은 세포 또는 단리된 효소 또는 폴리펩티드가 비교 가능한 동일 종의 세포 또는 그의 본래 폴리펩티드에서 측정된 활성 수준과 비교하여 낮은 활성 수준을 나타내거나 활성을 나타내지 않는 것을 의미한다. 즉 해당 폴리펩티드의 활성이 본래 조작되지 않은 폴리펩티드에 의한 동일한 생화학적 활성보다 약 10%이상, 약 20%이상, 약 30%이상, 약 40%이상, 약 50% 이상, 약 55% 이상, 약 60% 이상, 약 70% 이상, 약 75% 이상, 약 80% 이상, 약 85% 이상, 약 90% 이상, 약 95% 이상, 또는 약 100% 감소된 것일 수 있다. 감소된 효소 활성은 당업계에 공지된 임의의 방법을 사용하여 확인될 수 있다. 상기 활성의 감소는 효소가 발현되더라도 효소의 활성이 없거나 감소된 경우 또는 효소를 코딩하는 유전자가 발현되지 않거나 발현되더라도 본래 조작이 되지 않은 유전자에 비하여 발현량이 감소된 경우를 포함한다.
상기 효소의 활성이 감소되는 것은 상기 효소를 코딩하는 유전자의 제거 또는 파괴에 의한 것일 수 있다. 유전자의 "제거 (deletion)" 또는 "파괴 (disruption)"는 유전자가 발현되지 않거나 발현량이 감소되거나 발현되어도 효소 활성을 나타내지 않거나 활성이 감소되도록, 유전자의 일부 또는 전부가, 또는 그 프로모터, 그 터미네이터 영역 등의 조절 인자의 일부 또는 전부가 변이, 치환, 삭제되거나 유전자에 하나 이상의 염기가 삽입되는 것을 말한다. 상기 유전자의 제거 또는 파괴는 상동 재조합과 같은 유전자 조작, 돌연변이 유발, 분자 진화를 통해 달성될 수 있다. 세포가 복수 개의 같은 유전자를 포함하거나 2개 이상의 다른 폴리펩티드 동종상동유전자 (paralog)를 포함하는 경우, 하나 또는 그 이상의 유전자가 제거 또는 파괴될 수 있다.
본 발명의 핵산 또는 폴리펩티드의 "서열 동일성 (sequence identity)"은 특정 비교 영역에서 양 서열을 최대한 일치되도록 얼라인시킨 후 서열간의 염기 또는 아미노산 잔기의 동일한 정도를 의미한다. 서열 동일성은 특정 비교 영역에서 2개의 서열을 최적으로 얼라인하여 비교함으로써 측정되는 값으로서, 비교 영역 내에서 서열의 일부는 대조 서열 (reference sequence)과 비교하여 부가 또는 삭제되어 있을 수 있다. 서열 동일성 백분율은 예를 들면, 비교 영역 전체에서 두 개의 최적으로 정렬된 서열을 비교하는 단계, 두 서열 모두에서 동일한 아미노산 또는 핵산이 나타나는 위치의 갯수를 결정하여 일치된 (matched) 위치의 갯수를 수득하는 단계, 상기 일치된 위치의 갯수를 비교 범위 내의 위치의 총 갯수 (즉, 범위 크기)로 나누는 단계, 및 상기 결과에 100을 곱하여 서열 동일성의 백분율을 수득하는 단계에 의해 계산될 수 있다. 상기 서열 동일성의 퍼센트는 공지의 서열 비교 프로그램을 사용하여 결정될 수 있으며, 상기 프로그램의 일례로 BLASTN (NCBI), CLC Main Workbench (CLC bio), MegAlignTM (DNASTAR Inc) 등을 들 수 있다.
여러 종의 동일하거나 유사한 기능이나 활성을 가지는 폴리펩티드 또는 폴리뉴클레오티드를 확인하는데 있어서 여러 수준의 서열 동일성을 사용할 수 있다. 예를 들어, 50%이상, 55%이상, 60%이상, 65%이상, 70%이상, 75%이상, 80%이상, 85%이상, 90%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상 또는 100% 등을 포함하는 서열 동일성이다.
일 양상은 유전적으로 조작되지 않은 세포에 비하여 3-히드록시프로피온산 (3-HP)을 3-히드록시프로피오닐-CoA (3-HP-CoA)로 전환하는 것을 촉매하는 CoA transferase, 3-HP-CoA를 아크릴릴-CoA (acrylyl-CoA)로 전환하는 것을 촉매하는 3-HP-CoA 데히드라타제, 및 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소의 활성이 증가되어 있는, 아크릴레이트 생산능을 갖는 미생물을 제공하는 것이다.
상기 CoA transferase는 EC 2.8.3.8, EC 3.1.2.-, 또는 EC 6.2.1.17에 속하는 것일 수 있다. 상기 CoA transferase는 3-히드록시프로피온산 (3-HP)을 3-히드록시프로피오닐-CoA (3-HP-CoA)로 전환하는 것을 촉매하는 활성이 그 역반응을 촉매하는 것보다 높은 것일 수 있다. 상기 CoA transferase는 서열번호 1 내지 10의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. 상기 CoA transferase를 코딩하는 폴리뉴클레오티드는 서열번호 1 내지 10의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 코딩하는 것일 수 있다. 상기 CoA transferase를 코딩하는 폴리뉴클레오티드는 서열번호 11 내지 20의 뉴클레오티드 서열과 95%이상의 서열 동일성을 갖는 것일 수 있다. 상기 CoA transferase는 표 1에 나타낸 것 중 하나 이상인 것일 수 있다. 표 1의 효소는 모두 E1 타입일 수 있다.
| 번호 | EC | 카테고리 | 소스 균주 | 유전자명 |
균주
구입처 |
서열* |
| 1 | 2.8.3.8 | Acetate CoA-transferase | Clostridium propionicum | pct | KCTC5582 | 1/11 |
| 2 | 2.8.3.8 | Acetate CoA-transferase | Escherichia coli (strain K12) | ydiF b1694 JW1684 | 보유 | 2/12 |
| 3 | 2.8.3.8 | Acetate CoA-transferase | Cupriavidus necator | pct | KCTC22469 | 3/13 |
| 4 | 6.2.1.17 | CoA transferase | Halomonas smyrnensis | acuN | DSM21644 | 4/14 |
| 5 | 6.2.1.17 | CoA transferase | Ruegeria pomeroyi DSS-3 | SPO2934 | DSM15171 | 5/15 |
| 6 | 2.8.3.8 | Acetate CoA-transferase | Desulfosporosinus youngiae DSM 17734 | DesyoDRAFT_3698 | DSM17734 | 6/16 |
| 7 | 3.1.2.- | Thioesterase | Peptoniphilus indolicus ATCC 29427 | HMPREF9129_0351 | KCTC15023 | 7/17 |
| 8 | 2.8.3.8 | Acetate CoA-transferase | Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10) | Desmer_1798 | DSM13257 | 8/18 |
| 9 | 2.8.3.8 | Acetate CoA-transferase | Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628) (Desulfotomaculum orientis) | Desor_3090 | DSM765 | 9/19 |
| 10 | 2.8.3.8 | Acetate CoA-transferase | Peptostreptococcus anaerobius CAG:621 | BN738_00826 | KCTC5182 | 10/20 |
* 서열은 아미노산 서열번호/뉴클레오티드 서열번호를 나타낸다.
상기 3-HP-CoA 데히드라타제는 EC 4.2.1.17, EC 4.2.1.55, 및 EC 4.2.1.166를 포함한, EC 4.2.1.-에 속하는 것일 수 있다. 상기 3-HP-CoA 데히드라타제는 3-HP-CoA를 아크릴릴-CoA (acrylyl-CoA)로 전환하는 것을 촉매하는 활성이 그 역반응을 촉매하는 것보다 높은 것일 수 있다. 상기 3-HP-CoA 데히드라타제는 서열번호 21 내지 98, 및 401의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. 상기 3-HP-CoA 데히드라타제를 코딩하는 폴리뉴클레오티드는 서열번호 21 내지 98, 및 401의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 코딩하는 것일 수 있다. 상기 3-HP-CoA 데히드라타제를 코딩하는 폴리뉴클레오티드는 서열번호 99 내지 176, 및 402의 뉴클레오티드 서열과 95%이상의 서열 동일성을 갖는 것일 수 있다. 상기 3-HP-CoA 데히드라타제는 표 2 내지 표 5에 나타낸 것 중 하나 이상인 것일 수 있다. 표 2 내지 표 5에 나타낸 효소는 E2 타입일 수 있다. 표 2 내지 5에서 "서열*"은 아미노산 서열번호/뉴클레오티드 서열번호를 나타낸다.
| 번호 | EC | 카테고리 | 소스 균주 | 유전자 | 구입체 | 서열* |
| 1 | 4.2.1.- | 3-hydroxybutyryl-CoA dehydratase(Crotonase) | Dictyostelium discoideum (Slime mold) | Q869N6 | DSM947 | 21/99 |
| 2 | 4.2.1.55 | 3-hydroxybutyryl-CoA dehydratase(Crotonase) | Clostridium acetobutylicum | crt CA_C2712 | KCTC1790 | 22/100 |
| 3 | 4.2.1.55 | 3-hydroxybutyryl-CoA dehydratase(Crotonase) | Clostridium difficile | crt ech | KCTC5009 | 23/101 |
| 4 | 4.2.1.55 | 3-hydroxybutyryl-CoA dehydratase(Crotonase) | Clostridium pasteurianum | F502_09038 | KCTC1674 | 24/102 |
| 5 | 4.2.1.55 | 3-hydroxybutyryl-CoA dehydratase(Crotonase) | Clostridium pasteurianum | F502_06297 | KCTC1674 | 25/103 |
| 6 | 4.2.1.55 | 3-hydroxybutyryl-CoA dehydratase(Crotonase) | Megasphaera elsdenii | MELS_1449 | KCTC5187 | 26/104 |
| 7 | 4.2.1.116 | 3-hydroxybutyryl-CoA dehydratase(Crotonase) | Metallosphaera sedula | Msed_2001 | DSM5348 | 27/105 |
| 8 | 4.2.1.55 | 3-hydroxybutyryl-CoA dehydratase(Crotonase) | Clostridicum kluyvery | crt1 | DSM555 | 28/106 |
| 9 | 4.2.1.- | 4-hydroxybutyryl-CoA dehydratase | Sulfolobus tokodaii | STK_16590 | DSM16993 | 29/107 |
| 10 | 4.2.1.- | 4-hydroxybutyryl-CoA dehydratase | Geobacter metallireducens | Gmet_2215 | DSM7210 | 30/108 |
| 11 | 4.2.1.- | 4-hydroxybutyryl-CoA dehydratase | Sulfolobus solfataricus | abfD-1 | DSM1617 | 31/109 |
| 12 | 4.2.1.- | 4-hydroxybutyryl-CoA dehydratase | Syntrophobacter fumaroxidans | Sfum_3141 | DSM10017 | 32/110 |
| 13 | 4.2.1.- | 4-hydroxybutyryl-CoA dehydratase | Porphyromonas gingivalis | PGN_0727 | DSM20709 | 33/111 |
| 14 | 4.2.1.- | 4-hydroxybutyryl-CoA dehydratase | Polynucleobacter necessarius subsp. Asymbioticus | Pnuc_0370 | DSM18221 | 34/112 |
| 15 | 4.2.1.116 | 3-hydroxypropionyl-CoA dehydratase | Sulfolobus tokodaii | STK_15160 | DSM16993 | 35/113 |
| 16 | 4.2.1.- | 3-hydroxypropionyl-CoA dehydratase | Gordonia terrae C-6 | GTC6_11571 | KCTC9807 | 36/114 |
| 17 | 4.2.1.- | 3-hydroxypropionyl-CoA dehydratase | Halalkalicoccus jeotgali | HacjB3_17558 C497_07209 | DSM18796 | 37/115 |
| 18 | 4.2.1.- | 3-hydroxypropionyl-CoA dehydratase | Carboxydothermus hydrogenoformans | CHY_1739 | DSM6008 | 38/116 |
| 19 | 4.2.1.55 | 3-hydroxypropionyl-CoA dehydratase | Thermomicrobium roseum | trd_0041 | DSM5159 | 39/117 |
| 20 | 4.2.1.17 | 3-hydroxypropionyl-CoA dehydratase | Methylobacterium extorquens | croA METDI5699 | DSM1337 | 40/118 |
| 번호 | EC | 카테고리 | 소스 균주 | 유전자 | 구입처 | 서열* |
| 21 | 4.2.1.- | R-phenyllactate dehydratase | Clostridium sporogenes |
fldB | KCTC5654 | 41/119 |
| 22 | 4.2.1.- | R-phenyllactate dehydratase | fldC | KCTC5654 | 42/120 | |
| 23 | 4.2.1.- | R-phenyllactate dehydratase | fldI | KCTC5654 | 43/121 | |
| 24 | 4.2.1.- | R-phenyllactate dehydratase | fldA | KCTC5654 | 44/122 | |
| 25 | 4.2.1.- | R-phenyllactate dehydratase | Lachnoanaerobaculum saburreum |
fldC HMPREF0381_2734 | DSM3986 | 45/123 |
| 26 | 4.2.1.- | R-phenyllactate dehydratase | fldB HMPREF0381_2735 | DSM3986 | 46/124 | |
| 27 | 4.2.1.- | R-phenyllactate dehydratase | fldI2 HMPREF0381_2736 | DSM3986 | 47/125 | |
| 28 | 4.2.1.- | R-phenyllactate dehydratase | Peptostreptococcus stomatis |
fldI HMPREF0634_1391 | DSM17678 | 48/126 |
| 29 | 4.2.1.- | R-phenyllactate dehydratase | HMPREF0634_1028 | DSM17678 | 49/127 | |
| 30 | 4.2.1.- | R-phenyllactate dehydratase | fldB HMPREF0634_1029 | DSM17678 | 50/128 | |
| 31 | 4.2.1.- | 2-hydroxyisocaproyl-CoA dehydratase | Clostridium difficile |
hadB | KCTC5009 | 51/129 |
| 32 | 4.2.1.- | 2-hydroxyisocaproyl-CoA dehydratase | hadC | KCTC5009 | 52/130 | |
| 33 | 4.2.1.- | 2-hydroxyisocaproyl-CoA dehydratase | hadI | KCTC5009 | 53/131 | |
| 34 | 4.2.1.- | 2-hydroxyisocaproyl-CoA dehydratase | hadA | KCTC5009 | 54/132 | |
| 35 | 4.2.1.17 | Enoyl-CoA hydratase | Escherichia coli (strain K12) | paaF | 보유 | 55/133 |
| 36 | 4.2.1.17 | Enoyl-CoA hydratase | Rhodobacter capsulatus | fadB1 | KCTC2583 | 56/134 |
| 37 | 4.2.1.- | Enoyl-CoA hydratase | Pseudomonas stutzeri | PSTAA_0117 | DSM4166 | 57/135 |
| 38 | 4.2.1.- | Enoyl-CoA hydratase | Haliangium ochraceum | Hoch_4602 | DSM14365 | 58/136 |
| 39 | 4.2.1.- | Enoyl-CoA hydratase | Anoxybacillus flavithermus | Aflv_0566 | DSM21510 | 59/137 |
| 40 | 4.2.1.- | Enoyl-CoA hydratase | Streptomyces avermitilis | echA3 SAV_717 | DSM46492 | 60/138 |
| 41 | 4.2.1.- | Enoyl-CoA hydratase | Advenella kashmirensis | TKWG_10020 | DSM17095 | 61/139 |
| 번호 | EC | 카테고리 | 소스 균주 | 유전자 | 구입처 | 서열* |
| 42 | 4.2.1.- | Enoyl-CoA hydratase | Oligotropha carboxidovorans | OCA5_c12950 OCAR_6780 | DSM1227 | 62/140 |
| 43 | 4.2.1.- | Enoyl-CoA hydratase | Riemerella anatipestifer | Riean_1526 RA0C_1812 | DSM15868 | 63/141 |
| 44 | 4.2.1.- | Enoyl-CoA hydratase | Fusobacterium necrophorum subsp. funduliforme Fnf 1007 |
HMPREF1127_1435 | DSM19678 | 64/142 |
| 45 | 4.2.1.- | Enoyl-CoA hydratase | HMPREF1127_1434 | DSM19678 | 65/143 | |
| 46 | 4.2.1.- | Enoyl-CoA hydratase | HMPREF1127_1436 | DSM19678 | 66/144 | |
| 47 | 4.2.1.- | Enoyl-CoA hydratase | Desulfosporosinus youngiae DSM 17734 |
DesyoDRAFT_3696 | DSM17734 | 67/145 |
| 48 | 4.2.1.- | Enoyl-CoA hydratase | DesyoDRAFT_3695 | DSM17734 | 68/146 | |
| 49 | 4.2.1.- | Enoyl-CoA hydratase | DesyoDRAFT_3697 | DSM17734 | 69/147 | |
| 50 | 4.2.1.- | Enoyl-CoA hydratase | Peptoniphilus indolicus ATCC 29427 |
fldB HMPREF9129_0353 | KCTC15023 | 70/148 |
| 51 | 4.2.1.- | Enoyl-CoA hydratase | HMPREF9129_0354 | KCTC15023 | 71/149 | |
| 52 | 4.2.1.- | Enoyl-CoA hydratase | HMPREF9129_0352 | KCTC15023 | 72/150 | |
| 53 | 4.2.1.- | Enoyl-CoA hydratase | Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10) |
Desmer_1800 | DSM13257 | 73/151 |
| 54 | 4.2.1.- | Enoyl-CoA hydratase | Desmer_1801 | DSM13257 | 74/152 | |
| 55 | 4.2.1.- | Enoyl-CoA hydratase | Desmer_1799 | DSM13257 | 75/153 | |
| 56 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Acidaminococcus fermentans |
hgdA Acfer_1815 | DSM20731 | 76/154 |
| 57 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | hgdB Acfer_1815 | DSM20731 | 77/155 | |
| 58 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | hgdC Acfer_1815 | DSM20731 | 78/156 | |
| 59 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Carboxydothermus hydrogenoformans |
hgdB CHY_0846 | DSM6008 | 79/157 |
| 60 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | hgdA CHY_0847 | DSM6008 | 80/158 | |
| 61 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | hgdC CHY_0848 | DSM6008 | 81/159 | |
| 62 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Oscillibacter valericigenes |
hgdC OBV_10870 | DSM18026 | 82/160 |
| 63 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | hgdA OBV_10880 | DSM18026 | 83/161 | |
| 64 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | hgdB OBV_10890 | DSM18026 | 84/162 |
| 번호 | EC | 카테고리 | 소스 균주 | 유전자 | 구입처 | 서열* |
| 65 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628) (Desulfotomaculum orientis) |
Desor_3092 | DSM765 | 85/163 |
| 66 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Desor_3093 | DSM765 | 86/164 | |
| 67 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Desor_3091 | DSM765 | 87/165 | |
| 68 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Peptostreptococcus anaerobius CAG:621 |
BN738_00824 | KCTC5182 | 88/166 |
| 69 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | BN738_00823 | KCTC5182 | 89/167 | |
| 70 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | BN738_00825 | KCTC5182 | 90/168 | |
| 71 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Chloroflexus aggregans (strain MD-66 / DSM 9485) | Cagg_1174 | DSM9485 | 91/169 |
| 72 | 4.2.1.17 | 2-hydroxyglutaryl-CoA dehydratase | Marivirga tractuosa (strain ATCC 23168 / DSM 4126 / NBRC 15989 / NCIMB 1408 / VKM B-1430 / H-43) (Microscilla tractuosa) (Flexibacter tractuosus) | Ftrac_3721 | KCTC2958 | 92/170 |
| 73 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Marinithermus hydrothermalis (strain DSM 14884 / JCM 11576 / T1) | Marky_1278 | DSM14884 | 93/171 |
| 74 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Chitinophaga pinensis (strain ATCC 43595 / DSM 2588 / NCIB 11800 / UQM 2034) | Cpin_6304 | KCTC3412 | 94/172 |
| 75 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Megasphaera elsdenii DSM 20460 | MELS_0744 | KCTC5187 | 95/173 |
| 76 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Megasphaera elsdenii DSM 20460 | MELS_0745 | KCTC5187 | 96/174 |
| 77 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Megasphaera elsdenii DSM 20460 | MELS_0746 | KCTC5187 | 97/175 |
| 78 | 4.2.1.- | 2-hydroxyglutaryl-CoA dehydratase | Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) | Chy400_0108 | DSM635 | 98/176 |
| 79 | 4.2.1.- | enoyl-CoA hydrastase | Ruegeria pomeroyi DSS-3 | SP00147 | DSM15171 | 401/402 |
상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 EC 3.1.2.4를 포함한, EC 3.1.2-에 속하는 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 3-HP-CoA hydrolase, 또는 3-hydroxyisobutyryl-CoA hydrolase인 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 활성이 그 역반응을 촉매하는 것보다 높은 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 서열번호 177 내지 182의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소를 코딩하는 폴리뉴클레오티드는 서열번호 177 내지 182의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 코딩하는 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소를 코딩하는 폴리뉴클레오티드는 서열번호 183 내지 188의 뉴클레오티드 서열과 95%이상의 서열 동일성을 갖는 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 표 6에 나타낸 것 중 하나 이상인 것일 수 있다. 표 6에 나타낸 효소는 E3 타입일 수 있다. 표 6에서, "서열*"은 아미노산 서열번호/뉴클레오티드 서열번호를 나타낸다.
| 번호 | EC | 카테고리 | 소스 균주 | 유전자명 | 구입 | 서열* |
| 1 | 3.1.2.- | Acyl - CoA thioester hydrolase | E. coli | yciA | 보유 | 177/183 |
| 2 | 3.1.2.- | Acyl-CoA thioester hydrolase | Klebsiella oxytoca 10-5245 | HMPREF9689_01673 | KCTC1686 | 178/184 |
| 3 | 3.1.2.- | Acyl-CoA thioester hydrolase | Cronobacter turicensis | yciA | 보유 | 179/185 |
| 4 | 3.1.2.- | Acyl-CoA thioester hydrolase | Citrobacter freundii | D186_20262 | 보유 | 180/186 |
| 5 | 3.1.2.- | Acyl-CoA thioester hydrolase | Salmonella enterica | SeI_A1458 | DSM5569 | 181/187 |
| 6 | 3.1.2.- | Acyl-CoA thioester hydrolase | Shigella flexneri 1235-66 | SF123566_2028 | 보유 | 182/188 |
상기 미생물은 유전적으로 조작되지 않은 세포에 비하여 상기 3개 효소 유전자의 발현이 증가되도록 유전적으로 조작된 미생물일 수 있다. 상기 3개 효소의 활성이 모(parent) 세포에 이미 존재하는 경우에는 유전적 조작을 통해 상기 3개 효소의 발현을 더욱 증가시킬 수 있거나, 야생형 미생물에 존재하지 않는 경우에는 유전적 조작의 방법으로 상기 3개 효소를 코딩하는 유전자를 모세포에 도입하여 발현 또는 과발현되도록 할 수 있다. 상기 유전적으로 조작되지 않은 세포는 야생형 또는 상기 미생물이 유래된 모세포를 의미한다.
상기 3개 효소 유전자의 발현 또는 과발현은 당업자에게 알려진 여러 방법에 의해 달성될 수 있다. 일례로 유전자 카피 수를 증가시키거나, 유도제 (inducer) 또는 억제제 (repressor)와 같은 조절 물질을 사용하여 발현을 증가시킬 수 있다. 상기 카피 수 증가는 상기 유전자의 도입 또는 증폭에 의한 것일 수 있다. 즉, 작동조절 가능하도록 연결된 조절 인자 및 상기 3개 효소 유전자를 포함하는 벡터, 발현 카세트 등을 숙주세포에 도입함으로써 달성될 수 있다.
또는 3개 효소 활성의 증가는 상기 유전자의 발현 조절 서열의 변형에 의한 것일 수 있다. 상기 조절 서열은 상기 유전자 발현을 위한 프로모터 서열 또는 전사 종결자 서열일 수 있다. 또한, 상기 조절 서열은 유전자 발현에 영향을 줄 수 있는 모티프를 코딩하는 서열일 수 있다. 상기 모티프는 예를 들면, 이차 구조-안정화 모티프, RNA 불안정화 모티프, 스플라이스-활성화 모티프, 폴리아데닐화 모티프, 아데닌-풍부 서열 (adenine-rich sequence), 또는 엔도뉴클레아제 인식 부위일 수 있다.
상기 미생물은 박테리아, 효모, 및 곰팡이로 이루어진 군으로부터 선택될 수 있다. 예를 들면, 에세리키아 (Esherichia), 루멘박테리아, 코리네박테리움 (Corynebacterium) 속 및 브레비박테리움 (Brevibacterium) 속으로 구성된 군으로부터 선택되는 것일 수 있다. 상기 세포는 코리네박테리움 속일 수 있다. 상기 미생물은 대장균 (E.coli), 코리네박테리움 글루타미쿰 (Corynebacterium glutamicum), 코리네박테리움 써모아미노게네스 (Corynebacterium thermoaminogenes), 브리비박테리움 플라붐 (Brevibacterium flavum) 및 브리비박테리움 락토페르멘툼 (Brevibacterium lactofermentum)으로 구성된 군으로부터 선택되는 미생물일 수 있다.
상기 미생물은 아크릴산을 천연으로 생산하거나 또는 재조합 방법으로 아크릴산을 생산하도록 유전적으로 조작된 것일 수 있다. 또한, 상기 미생물은 3-HP 생산능을 갖는 것일 수 있다. 상기 미생물이 천연적으로 3-HP를 생산하지 하지 않는 경우, 3-HP를 생산하도록 유전적으로 조작된 것일 수 있다. 상기 미생물은 글리세롤을 3-프로피온산 알데히드 (3-propionic aldehyde: 3-HPA)로 전환하는 반응을 촉매하는 효소를 코딩하는 유전자, 및 3-HPA를 3-HP로 전환하는 반응을 촉매하는 효소를 코딩하는 유전자가 도입되어 있어, 3-HP 생산능을 갖는 것일 수 있다. 상기 미생물은 예를 들면, 대장균을 포함한 에세리키아 속 미생물일 수 있다. 상기 글리세롤을 3-HPA로 전환하는 반응을 촉매하는 효소는 글리세롤 데히드라타제 (glycerol dehydratase: GDH)일 수 있다. 상기 3-HPA를 3-HP로 전환하는 반응을 촉매하는 효소는 알데히드 데히드로게나제(aldehyde dehydrogenase: ALD)일 수 있다.
상기 GDH는 글리세롤을 3-HPA로의 전환을 촉매하는 것이면 어느 것이 포함될 수 있다. 상기 GDH는 EC 4.2.1.30 또는 디올 데히드라타제 (EC 4.2.1.28)에 속하는 것일 수 있다. 상기 GDH 및 그를 코딩하는 뉴클레오티드는 Ilyobacter polytropus, Klebsiella pneumoniae, Citrobacter freundii, Clostritidium pasteurianum, Salmonella typhimurium, 또는 Klebsiella oxytoca로부터 유래한 것일 수 있다. 각 경우에 있어서, 상기 GDH는 세 개 서브유닛으로 구성될 수 있다: 큰 (large) 또는 "α" 서브유닛, 중간 (medium) 또는 "β" 서브유닛, 및 작은 (small) 또는 "γ" 서브유닛. GDH의 큰 (large) 또는 "α" 서브유닛을 코딩하는 유전자는 dhaB1, gldA 및 dhaB를 포함할 수 있다. 중간 (medium) 또는 "β" 서브유닛를 코딩하는 유전자는 dhaB2, gldB 및 dhaC를 포함할 수 있다. 작은 (small) 또는 "γ" 서브유닛을 코딩하는 유전자는 dhaB3, gldC 및 dhaE를 포함할 수 있다. 디올 데히드라타제의 큰 (large) 또는 "α" 서브유닛을 코딩하는 유전자는 pduC 및 pddA를 포함할 수 있다. 디올 데히드라타제의 중간 (medium) 또는 "β" 서브유닛를 코딩하는 유전자는 pduD 및 pddB를 포함할 수 있다. 디올 데히드라타제의 작은 (small) 또는 "γ" 서브유닛을 코딩하는 유전자는 pduE 및 pddC를 포함할 수 있다. 표 7 및 표 8은 GDH 및 GDH 연결된 기능에 대한 유전자 명칭 및 GenBank 참조를 비교한 것이다. 상기 GDH는 Ilyobacter polytropus 유래의 dhaB1, dhaB2, 및 dhaB3를 포함하는 것일 수 있다. Ilyobacter polytropus 유래의 DhaB1, DhaB2, 및 DhaB3는 각각 서열번호 183,184,및 185의 아미노산 서열을 갖는 것일 수 있다. dhaB1 유전자, dhaB2 유전자, 및 dhaB3 유전자는 각각 서열번호 183,184,및 185의 아미노산 서열을 코딩하는 것일 수 있다. Ilyobacter polytropus 유래의 dhaB1 유전자, dhaB2 유전자, 및 dhaB3 유전자는 각각 서열번호 186, 187, 및 188을 갖는 것일 수 있다.
| 개체 (GenBank참조번호) |
유전자 기능 | |||||||
| 조절 |
미지 |
재활성화 (reactivation) |
미지 |
|||||
| 유전자 | 염기쌍 | 유전자 | 염기쌍 | 유전자 | 염기쌍 | 유전자 | 염기쌍 | |
| K.pneumoniae(U30903) | orf2c | 7116-7646 | orf2b | 6762-7115 | orf2a | 5125-5556 | ||
| K.pneumoniae(U60992) | GdrB | |||||||
| C.freundii(U09771) | dhaR | 3746-5671 | orfW | 5649-6179 | orfX | 6180-6533 | orfY | 7736-8164 |
| C.pasteurianum(AF051373) | ||||||||
| C.pasteurianum(AF026270) | orfW | 210-731 | orfX | 1-196 | orfY | 746-1177 | ||
| S.typhimurium(AF026270) | pduH | 8274-8645 | ||||||
| K.oxytoca(AF017781) | DdrB | 2063-2440 | ||||||
| K.oxytoca(AF051373) | ||||||||
| 개체 (GenBank참조번호) |
유전자 기능 | |||||||
| 데히드라타제,α | 데히드라타제,α |
데히드라타제,α | 재활성화 (reactivation) |
|||||
| 유전자 | 염기쌍 | 유전자 | 염기쌍 | 유전자 | 염기쌍 | 유전자 | 염기쌍 | |
| K.pneumoniae(U30903) | dhaB1 | 3047-4714 | dhaB2 | 2450-2890 | dhaB3 | 2022-2447 | orf2a | 186-2009 |
| K.pneumoniae(U60992) | gldA | 121-1788 | gldB | 1801-2382 | gldB | 2388-2813 | gdrA | |
| C.freundii(U09771) | dhaB | 8556-10223 | dhaC | 10235-10819 | dhaC | 10822-11250 | orfY | 11261-13072 |
| C.pasteurianum(AF051373) | dhaB | 84-1748 | dhaC | 1779-2318 | dhaC | 2333-2773 | 2790-4598 | |
| C.pasteurianum(AF026270) | orfY | |||||||
| S.typhimurium(AF026270) | pduC | 3557-5221 | pduD | 5232-5906 | pduD | 5921-6442 | 6452-8284 | |
| K.oxytoca(AF017781) | 241-2073 | |||||||
| K.oxytoca(AF051373) | pddA | 121-1785 | pddB | 1796-2470 | pddB | 2485-3006 | ||
상기 GDH는 Ilyobacter polytropus 유래의 dhaB1, dhaB2, 및 dhaB3의 각 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다.
상기 ALD는 3-HPA를 3-HP로의 전환을 촉매하는 단백질이면 어느 것이 포함될 수 있다. 상기 ALD는 NAD, NADP, FAD, 또는 PQQ과 같은 레독스 조요소 (redox cofactor)를 사용할 수 있다. 상기 ALD는 EC 1.2.1.3 (NAD-의존성), EC 1.2.1.4 (NADP-의존성), EC 1.2.99.3 (PQQ-의존성), 또는 EC 1.2.99.7 (FAD-의존성)일 수 있다. NADP-의존성 ALD의 예는 대장균 유전자 aldB에 의하여 코딩되는 AldB일 수 있다. NAD-의존성 ALD의 예는 대장균 유전자 aldA에 의하여 코딩되는 AldA 또는 대장균 유전자 aldH에 의하여 코딩되는 AldH일 수 있다. 상기 ALD는 숙시네이트 세미알데히드 데히드로게나제 (succinate semialdehyde dehydrogenase: SSADH)일 수 있다. SSADH는 EC 1.2.1.24 또는 EC 1.2.1.16에 속하는 것일 수 있다. 상기 SSADH는 NAD+, NADP+ 또는 둘 다에 의존성인 것일 수 있다. SSADH는 CoA 비의존성 (independent)한 것일 수 있다. SSADH는 예를 들면, 코리네박테리움 속, 로도코쿠스 속, 고르도니아 속(Gordonia sp.), 미코박테리움 속, 안트로박터 속, 또는 에세리카아 속 유래일 수 있다. 상기 SSADH는 대장균 유래의 gabD1, gabD2, 또는 gabD3일 수 있다. 상기 SSADH를 코딩하는 유전자는 예를 들면, 서열번호 189, 190, 및 191의 아미노산 서열을 코딩하는 폴리뉴클레오티드일 수 있다. 상기 SSADH는 Cupriavidus necator 유래의 gabD (뉴클레오티드 서열: 서열번호 192, 아미노산 서열: 서열번호 193)일 수 있다. 상기 SSADH를 코딩하는 유전자는 예를 들면, 서열번호 189, 190, 191, 및 193의 아미노산 서열을 코딩하는 폴리뉴클레오티드일 수 있다. 상기 숙시닐 세미알데히드 데히드로게나제를 코딩하는 유전자는 예를 들면, 서열번호 194, 195, 196 및 192의 뉴클레오티드 서열을 가질 수 있다. 상기 SSADH는 서열번호 189, 190, 191, 및 193의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다.
상기 미생물은 글리세롤 데하이드라타제 재활성화효소 (glycerol dehydratase reactivase: GDR)를 코딩하는 폴리뉴클레오티드를 더 포함하는 것일 수 있다. 글리세롤 및 디올 데히드라타제는 글리세롤 및 다른 일부 기질에 의한 기작-근거한 자살 불활성화의 대상이 된다 (Daniel et al., FEMS Microbiol. Rev. 22, 553(1999)). 용어 "글리세롤 데하이드라타제 재활성화효소 (glycerol dehydratase reactivase: GDR)"는 상기 데히드라타제 활성을 재활성화하는 단백질을 나타낸다. 용어 "데히드라타제 재활성화 활성 (dehydratase reactivating activity)"은 기질을 촉매할 수 없는 데히드라타제를 기질을 촉매할 수 있는 것으로 전환하는 현상 또는 데히드라타제의 저해를 억제하는 현상 또는 인 비보에서 데히드라타제 효소의 유용한 반감기 (half-life)를 연장시키는 현상을 나타낸다. 상기 GDR은 dhaB, gdrA, pduG 및 ddrA 중 하나 이상일 수 있다. 또한, GDR은 orfX, orf2b, gdrB, pduH 및 ddrB 중 하나 이상일 수 있다.
상기 GDR은 K.pneumoniae (U60992) 유래의 gdrA 및 gdrB로서 각각 서열번호 197 및 198의 아미노산 서열을 갖는 것일 수 있다. 또는, 상기 GDR은 Ilyobacter polytropus 유래의 gdrA 및 gdrB로서 각각 서열번호 199 및 200의 아미노산 서열을 갖는 것일 수 있다. 상기 GDR은 서열번호 197, 198, 199 및 200의 아미노산 서열과 각각 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. GdrA 및 GdrB을 코딩하는 유전자는 각각 서열번호 197, 198, 199 및 200의 아미노산 서열을 코딩하는 서열을 갖는 것, 예를 들면, 서열번호 201, 202, 203 및 204의 뉴클레오티드 서열을 갖는 것일 수 있다.
상기 미생물에 있어서, GDH를 코딩하는 폴리뉴클레오티드, ALD를 코딩하는 폴리뉴클레오티드 및 GDR을 코딩하는 폴리뉴클레오티드 중 하나 이상은 유전적으로 조작되지 않은 미생물에 비하여 더 높은 수준으로 발현되는 것일 수 있다. 상기 발현 수준은 mRNA 또는 단백질 수준의 발현일 수 있다. 상기 단백질 수준의 발현은 발현된 단백질의 양 또는 활성에 근거한 것일 수 있다. 상기 발현 수준은 약 5% 이상, 약 10% 이상, 약 15% 이상, 약 20% 이상, 약 30% 이상, 약 50% 이상, 약 60% 이상, 약 70% 이상, 약 100% 이상, 200% 이상, 또는 300% 이상 증가된 것일 수 있다.
상기 미생물은 3-HP 생산능을 갖는 것일 수 있다. 상기 미생물에 있어서, 상기 발현 증가는 유전적으로 조작되지 않은 미생물에 비하여 더 높은 수준의 3-HP를 생산하도록 하는 것일 수 있다. 상기 3-HP의 생산은 세포 내에서 생산되는 것, 세포 내에서 생산되어 세포 외부로 분비되는 것, 또는 그 조합을 포함한다. 세포 내에서 생산된 3-HP은 아크릴산과 같은 다른 대사 산물로부터 전환될 수 있다. 상기 3-HP 생산은 약 5% 이상, 약 10% 이상, 약 15% 이상, 약 20% 이상, 약 30% 이상, 약 50% 이상, 약 60% 이상, 약 70% 이상, 약 100% 이상, 200% 이상, 또는 300% 이상 증가된 것일 수 있다.
상기 발현 증가는 폴리펩티드를 코딩하는 폴리뉴클레오티드가 세포에 도입되거나 세포 내 카피 수가 증가되거나, 또는 상기 폴리뉴클레오티드의 조절 영역의 변이에 의한 것일 수 있다. 외부에서 도입되거나 또는 카피 수가 증가되는 폴리뉴클레오티드는 내인성 (endogenous) 또는 외인성 (exogenous)일 수 있다. 상기 내인성 유전자는 미생물 내부에 포함된 유전물질 상에 존재하던 유전자를 말한다. 외인성 유전자는 숙주 세포 게놈으로 도입 (integration)되는 등의 숙주 세포 내로 유전자가 도입되는 것을 의미하며, 도입되는 유전자는 도입되는 숙주세포에 대해 동종 (homologous) 또는 이종 (heterologous)일 수 있다.
상기 미생물은 아크릴레이트를 분해하거나 다른 산물로 전환하는 경로에 관여하는 하나 이상의 효소의 활성이 감소된 것일 수 있다. 상기 미생물은 아크릴레이트를 분해하거나 다른 산물로 전환하는 경로에 관여하는 하나 이상의 효소를 코딩하는 유전자가 제거 또는 파괴되어 있는 것일 수 있다.
또한, 상기 미생물은 아크릴레이트를 다른 산물로 전환하는 경로를 더 포함하는 것일 수 있다. 상기 미생물에 있어서, 아크릴산의 생산은 세포내, 또는 세포 내에서 생성되어 분비되는 것일 수 있다. 따라서, 상기 미생물은 아크릴산을 세포 내에 생성하고, 이를 다른 산물로 전환하는데 관여하는 경로, 예를 들면, 효소 유전자 및 그 발현물을 더 포함할 수 있다. 상기 다른 산물은 아크릴레이트 에스테르일 수 있다.
상기 미생물은 피루베이트로부터 락테이트를 합성하는 경로가 불활성화 또는 감쇄된 것일 수 있다. 상기 미생물은 락테이트 데히드로게나아제(lactate dehydrogenase, LDH)의 활성이 제거되거나 감소된 것일 수 있다. 상기 LDH는 피루베이트를 락테이트로 전환하는 반응을 촉매하는 활성을 가질 수 있다. 상기 LDH는 EC.1.1.1.27로 분류되는 효소일 수 있다. 일례로 상기 LDH는 서열번호 205의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. 상기 미생물은 락테이트 데히드로게나아제를 코딩하는 유전자가 파괴 또는 제거된 것일 수 있다. 상기 LDH 유전자는 서열번호 205의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 코딩하는 것일 수 있다.
다른 양상은 상기한 바와 같은 미생물을 배지 중에서 배양하는 단계;를 포함하는, 아크릴레이트를 생산하는 방법을 제공한다.
상기 배양은 당업계에 알려진 적당한 배지와 배양조건에 따라 이루어질 수 있다. 통상의 기술자라면 선택되는 미생물에 따라 배지 및 배양조건을 용이하게 조정하여 사용할 수 있다. 배양 방법은 회분식, 연속식, 유가식, 또는 이들의 조합 배양을 포함할 수 있다. 상기 미생물은 아크릴레이트를 세포 외부로 분비하는 것일 수 있다.
상기 배지는 다양한 탄소원, 질소원 및 미량원소 성분을 포함할 수 있다.
상기 탄소원은, 예를 들면, 포도당, 자당, 유당, 과당, 말토오스, 전분, 셀룰로오스와 같은 탄수화물, 대두유, 해바라기유, 피마자유, 코코넛유와 같은 지방, 팔미트산, 스테아린산, 리놀레산과 같은 지방산, 글리세롤 및 에탄올과 같은 알코올, 아세트산과 같은 유기산, 또는 이들의 조합을 포함할 수 있다. 상기 배양은 글루코스를 탄소원으로 하여 수행될 수 있다. 상기 질소원은, 펩톤, 효모 추출물, 육즙, 맥아 추출물, 옥수수 침지액 (CSL), 및 대두밀과 같은 유기 질소원 및 요소, 황산암모늄, 염화암모늄, 인산암모늄, 탄산암모늄 및 질산암모늄과 같은 무기 질소원, 또는 이들의 조합을 포함할 수 있다. 상기 배지는 인의 공급원으로서, 예를 들면, 인산이수소칼륨, 인산수소이칼륨 및 상응하는 소듐-함유 염, 황산마그네슘 또는 황산철과 같은 금속염을 포함할 수 있다. 또한, 아미노산, 비타민, 및 적절한 전구체 등이 배지에 포함될 수 있다. 상기 배지 또는 개별 성분은 배양액에 회분식, 유가식 또는 연속식으로 첨가될 수 있다.
또한, 배양 중에 달리 pH를 조절하지 않거나, 수산화암모늄, 수산화칼륨, 암모니아, 인산 및 황산과 같은 화합물을 미생물 배양액에 적절한 방식으로 첨가하여 배양액의 pH를 조정할 수 있다. 또한, 배양 중에 지방산 폴리글리콜 에스테르와 같은 소포제를 사용하여 기포 생성을 억제할 수 있다.
상기 배양은 미호기성 조건에서 배양하는 것일 수 있다. 용어 "미호기성 조건 (microaerobic condition)"은 정상 대기상태보다 산소의 함량이 적은 양의 산소를 가진 공기가 배양액과 접촉하는 상태에서 배양액에 공급되는 산소의 양을 의미한다. 미호기성 조건은 예를 들면, 대기중 공기에 이산화탄소 또는 질소를 약 0.1 내지 0.4 vvm, 약 0.2 내지 0.3 vvm, 또는 약 0.25 vvm의 유속으로 공급하여 조성될 수 있다. 또한, 미호기성 조건은 통기 속도가 약 0 내지 0.4 vvm, 약 0.1 내지 0.3 vvm, 또는 약 0.15 내지 0.25 vvm인 것일 수 있다.
상기 방법은 배양물로부터 아크릴레이트를 회수하는 단계를 더 포함할 수 있다. 상기 회수는 세포 또는 세포를 제외한 배양액, 또는 둘 모두에서 분리될 수 있다. 배양물로부터 아크릴산의 분리는 당업계에 알려진 분리 및 정제방법을 사용하여 수행될 수 있다. 상기 회수는 원심분리, 크로마토그래피, 추출, 여과, 침전, 또는 이들의 조합에 의하여 이루어질 수 있다.
상기 방법에 있어서, 상기 미생물은 아크릴레이트를 다른 산물로 전환하는 경로를 더 포함하는 것이고, 생산된 아크릴레이트를 다른 산물로 전환하는 단계를 더 포함하는 것일 수 있다. 다른 산물은 폴리아크릴레이트를 포함한 아크릴레이트 에스테르인 것일 수 있다.
일 양상에 따른 미생물에 의하면, 3-아크릴산의 증가된 생산능을 갖는다.
다른 양상에 따른 아크릴산을 생산하는 방법에 의하면, 아크릴산을 효율적으로 생산할 수 있다.
도 1은 pETDuet/dhaB_gdrAB_gabD 벡터의 개열 지도를 나타낸다.
도 2 및 도 3은 pETDuetTM-1/MELS_1449_yciA_YdiF의 지도 및 부분 확대된 도면이다.
도 4는 본 실시예의 대장균에서 포도당 또는 글리세롤로부터 아크릴산의 생산의 예측 경로를 나타낸 도면이다.
도 2 및 도 3은 pETDuetTM-1/MELS_1449_yciA_YdiF의 지도 및 부분 확대된 도면이다.
도 4는 본 실시예의 대장균에서 포도당 또는 글리세롤로부터 아크릴산의 생산의 예측 경로를 나타낸 도면이다.
이하 본 발명을 실시예를 통하여 보다 상세하게 설명한다. 그러나, 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.
실시예
1: 3-
HP
로부터, 3-
HP
-
CoA
,
AA
-
CoA
및
AA
로 전환하는 효소 활성의 확인
본 실시예에서는 3-HP로부터, 3-HP-CoA, AA-CoA 및 AA로 전환하는 각 효소 활성의 확인하였다. 즉, 3-HP를 3-HP-CoA로 전환하는 반응을 촉매하는 효소, 3-HP-CoA를 AA-CoA로 전환하는 반응을 촉매하는 효소, AA-CoA를 AA로 전환하는 반응을 촉매하는 효소의 활성을 인 비트로에서 확인하였다. 그 결과, 위에서 언급된 반응이 역 반응에 비하여 더 우세하게 일어나도록 촉매하는 효소들을 선별하였다.
(1) 3-
HP
를 3-
HP
-
CoA
로 전환하는 반응을
촉매하는
효소의 확인
먼저, 하기 표 1에 열거된 3-HP를 3-HP-CoA로 전환하는 반응을 촉매하는 CoA-transferase 유전자를 표 1에 언급된 미생물의 게놈을 주형으로 하고, HindIII와 BamHI 제한 부위 (restriction stie)가 존재하는 각 프라이머 세트를 사용한 PCR을 통하여 증폭하였다. PCR은 95℃에서 30초, 50℃에서 30초, 및 72℃에서 1분을 30회 (cycle) 수행하였다. 표 9는 사용된 프라이머를 나타낸다.
| 번호 | 포워드 / 리버스 프라이머 (서열번호) |
| 1 | 206/207 |
| 2 | 208/209 |
| 3 | 210/211 |
| 4 | 212/213 |
| 5 | 214/215 |
| 6 | 216/217 |
| 7 | 218/219 |
| 8 | 220/221 |
| 9 | 222/223 |
| 10 | 224/225 |
얻어진 각 증폭 산물을 HindIII 및 BamHI으로 소화시키고, 이를 pETDuetTM-1 벡터 (Novagen, cat. no. 71146-3)의 HindIII 및 BamHI 자리에 연결시켜, CoA-transferase 유전자 발현용 벡터를 각각 제조하였다 (이하 pETDuetTM-1(CT) 벡터라 함). 상기 발현용 벡터에서 각 효소는 벡터 중 His-Tag 코딩 서열과 작동가능하게 연결되어, 발현 산물은 His-tag이 융합된 형태로 발현되도록 한다.
얻어진 pETDuetTM-1(CT) 벡터를 대장균 BL21(DE3)에 형질전환에 의하여 도입하였다. 형질전환된 대장균을 LB 배지에 접종하고 100mM 플라스크에서 37℃에서 배양하였다. 배양 중 OD600= 0.6-0.8일 때, 1mM IPTG를 첨가하여 도입된 CoA transferase 유전자의 발현을 유도하였다. 발현 유도 24 시간 후 원심분리에 의하여 세포만을 분리한 후, 용액 1 (50mM NaH2PO4, 0.5M NaCl, pH8) 중에서 얼음에 넣고 초음파를 조사하여 세포를 파쇄하였다. 원심분리를 통하여 세포 파쇄물으로부터 상층액만을 얻은 후, Ni-NTA Sepharose High Performance 레진 (GE Healthcare Bio-Sciences AB)을 이용하여 제조사의 지시대로 용액 1 중 200mM imidazole 하에서 각 단백질과 레진으로부터 분리하여, 200mM imidazole 수용액 중 고순도의 각 효소 용액을 얻었다.
각 CoA transferase의 활성은 다음과 같이 분석하였다. 구체적으로, 100 mM Tris-HCl (pH 8.4), 2mM MgCl2, 3 mM ATP, 및 15 mM CoA 함유 수용액, 상기 얻어진 각 효소 용액 (10 mg CoA transferase에 해당하는 양) 및 기질로 사용되는 10mM 3-HP를 첨가한 후, 약 2 내지 10분 동안 반응시켰다. 추정되는 반응식은 다음과 같다.
3-HP + acetyl-CoA + ATP + CoA transferase -> 3-HP-CoA + acetate + CoA transferase
다음으로, 각 반응물에 대하여, HPLC 분석을 수행하여, 반응물인 acetyl-CoA와 생성물인 3-HP-CoA의 피크 및 그 양을 확인하였다. HPLC 분석은 Aminex HPX-87H (300 mm x 7.8 mm) 칼럼을 사용하였고, 이동상은 9% 아세토니트릴이 함유된 0.5 mM 황산용액을 사용하였다. 유속은 0.4 ml/분이고 칼럼의 온도는 35℃이었으며, 검출기는 RI 및 UV/VIS (210 nm) 듀얼모드를 이용하였다.
또한, 상기 각 반응물에 대하여, citrate synthase를 반응시켜, 남은 acetyl-CoA를 CoA로 전환시키고, 여기에 0.5 mM DTNB (5,5'-Dithiobis-(2-Nitrobenzoic Acid) 또는 Ellman's reagent)를 첨가한 후 412 nm에서 흡광도를 측정하였다. DTNB는 흡광도 측정에 의하여, 물질 중의 티올 (thiols)의 양을 측정하는데 사용될 수 있다. DTNB는 티올에 의하여 혼합된 disulfide를 쉽게 형성하고, 발색단 (chromophore) 5-merapto-2-nitrobenzoic acid (410 nm에서 최대 흡광도)를 방출한다. 이 수용성 시약에 접근가능한 물질 중의 티올만이 개질 될 수 있다 그 결과 얻어진, 남은 acetyl-CoA의 양을 비교하여 각 효소의 활성을 확인하였다.
표 10은 분석결과 acetyl-CoA와 3-HP-CoA의 최초 대비 남은 양을 나타낸다.
| 번호 | 유전자명 | 소비된 acetyl - CoA (몰%) |
| 1 | pct | 62±4.0 |
| 2 | ydiF b1694 JW1684 | 66±4.0 |
| 3 | pct | 53±3.0 |
| 4 | acuN | 17±4.4 |
| 5 | SPO2934 | 32±2.7 |
| 6 | DesyoDRAFT_3698 | 37±2.4 |
| 7 | HMPREF9129_0351 | 33±3.8 |
| 8 | Desmer_1798 | 28±4.9 |
| 9 | Desor_3090 | 27±3.6 |
| 10 | BN738_00826 | 35±3.2 |
표 10에 나타낸 바와 같이, acetyl-CoA 양은 줄어들면서, 3-HP-CoA 양은 증가하였으며, 그 결과 상기 10개 효소는 3-HP를 3-HP-CoA로 전환하는 반응을 촉매하는 것을 확인하였다.
(2) 3-
HP
-
CoA
를
AA
-
CoA
로 전환하는 반응을
촉매하는
효소의 확인
먼저, 표 2 내지 표 5에 열거된 3-HP-CoA를 AA-CoA로 전환하는 반응을 촉매하는 dehydratase 유전자를 표 2 내지 표 5에 언급된 미생물의 게놈을 주형으로 하고, HindIII 및 BamHI 자리를 가진 각 프라이머 세트를 사용한 PCR을 통하여 증폭하고, pETDuetTM-1 벡터 (Novagen, cat. no. 71146-3) 대신 pACYCDuetTM-1 벡터 (Novagen, cat. no. 71147-3)을 사용한 것을 제외하고는, (1)과 동일한 과정에 의하여 dehydratase 효소를 생산하고 정제하였다. 표 11은 사용된 프라이머 세트에 대한 정보를 나타낸다. 표 11에서 번호는 표 2 내지 5에 나타낸 효소 또는 유전자 일련 번호를 나타낸다.
| 번호 | 서열번호: 포워드/리버스 |
번호 | 서열번호: 포워드/리버스 |
번호 | 서열번호: 포워드/리버스 |
| 1 | 226/227 | 28 | 280/ 281 | 55 | 334/335 |
| 2 | 228/229 | 29 | 282/ 283 | 56 | 336/337 |
| 3 | 230/231 | 30 | 284/285 | 57 | 338/339 |
| 4 | 232/233 | 31 | 286/287 | 58 | 340/341 |
| 5 | 234/235 | 32 | 288/289 | 59 | 342/343 |
| 6 | 236/237 | 33 | 290/291 | 60 | 344/345 |
| 7 | 238/239 | 34 | 292/293 | 61 | 346/347 |
| 8 | 240/241 | 35 | 294/295 | 62 | 348/349 |
| 9 | 242/243 | 36 | 296/297 | 63 | 350/351 |
| 10 | 244/245 | 37 | 298/299 | 64 | 352/353 |
| 11 | 246/247 | 38 | 300/301 | 65 | 354/355 |
| 12 | 248/249 | 39 | 302/303 | 66 | 356/357 |
| 13 | 250/251 | 40 | 304/305 | 67 | 358/359 |
| 14 | 252/253 | 41 | 306/307 | 68 | 360/361 |
| 15 | 254/255 | 42 | 308/309 | 69 | 362/363 |
| 16 | 256/257 | 43 | 310/311 | 70 | 364/365 |
| 17 | 258/259 | 44 | 312/313 | 71 | 366/367 |
| 18 | 260/261 | 45 | 314/315 | 72 | 368/369 |
| 19 | 262/263 | 46 | 316/317 | 73 | 370/371 |
| 20 | 264/265 | 47 | 318/319 | 74 | 372/373 |
| 21 | 266/267 | 48 | 320/321 | 75 | 374/375 |
| 22 | 268/269 | 49 | 322/323 | 76 | 376/377 |
| 23 | 270/271 | 50 | 324/325 | 77 | 378/379 |
| 24 | 272/273 | 51 | 326/327 | 78 | 380/381 |
| 25 | 274/275 | 52 | 328/329 | 79 | 403/404 |
| 26 | 276/277 | 53 | 330/331 | ||
| 27 | 278/279 | 54 | 332/333 |
정제된 각 dehydratase의 활성은 다음과 같이 분석하였다. dehydratase, 예를 들면, 3-HP-CoA/lactoyl-CoA dehydratase가 촉매하는 반응은 반응 자체의 반응물 또는 산물을 측정하는 방법 대신, 반응물에 acrylyl-CoA reductase를 함께 첨가하여, propionyl-CoA가 생성되도록 하여, 소모되는 NAD(P)H의 양을 측정하였다. 구체적으로, 100 mM MOPS (3-(N-morpholino)propanesulfonic acid)-KOH (pH 7.0), 10 mM MgCl2, 3 mM ATP, 0.1 mM CoA, 및 20mM 3-HP 함유 수용액 0.5 ml에, 0.05-0.2 unit CoA transferase (표 1의 2번에 기재된 E.coli 유래의 것)를 첨가한 후, 약 3분 동안 예비 반응(pre-incubation)시켰다. 그 후 정제된 상기 각 dehydratase (10mM)를 첨가하고 5분 동안 35℃ 동안 반응시켰다. 추정되는 반응식은 다음과 같다.
3-HP + acetyl-CoA + ATP + CoA transferase -> 3-HP-CoA + acetate + dehydratase -> AA-CoA + NAD(P)H + Acrylyl-CoA reductase (YhdH) -> propionyl-CoA + NAD(P)
다음으로, 반응 결과로서 5mM NAD(P)H/AcuI첨가에 따른 NAD(P)H 양의 감소를 확인하였다. AcuI는 acrylyl-CoA reducatase를 나타내며, YhdH와 유사한 것으로 부가하여 첨가하였다. 최종 NAD(P)H 측정은 340nm에서의 흡광도를 측정하여 확인하였다.
표 12는 분석결과 확인된 NAD(P)H의 최초 대비 남은 양 (즉, 효소 활성의 정도)을 나타낸다.
| 번호 | 소비된 NAD(P)H(몰%) | 번호 | 소비된 NAD(P)H(몰%) | 번호 | 소비된 NAD(P)H(몰%) |
| 1 | 0.2 | 28 | 0.2 | 55 | 0.2 |
| 2 | 10.3 | 29 | 0.2 | 56 | 0.2 |
| 3 | 0.2 | 30 | 0.2 | 57 | 0.2 |
| 4 | 12.1 | 31 | 0.2 | 58 | 0.2 |
| 5 | 12.1 | 32 | 0.2 | 59 | 0.2 |
| 6 | 13.4 | 33 | 0.2 | 60 | 0.2 |
| 7 | 2.3 | 34 | 0.2 | 61 | 0.2 |
| 8 | 0.2 | 35 | 0.2 | 62 | 0.2 |
| 9 | 0.2 | 36 | 0.2 | 63 | 0.2 |
| 10 | 0.2 | 37 | 0.2 | 64 | 0.2 |
| 11 | 0.2 | 38 | 1.65 | 65 | 0.2 |
| 12 | 0.2 | 39 | 14.6 | 66 | 0.2 |
| 13 | 0.2 | 40 | 0.2 | 67 | 0.2 |
| 14 | 0.2 | 41 | 0.2 | 68 | 0.2 |
| 15 | 0.2 | 42 | 0.2 | 69 | 0.2 |
| 16 | 0.2 | 43 | 0.2 | 70 | 0.2 |
| 17 | 0.2 | 44 | 0.2 | 71 | 0.2 |
| 18 | 0.2 | 45 | 0.2 | 72 | 0.2 |
| 19 | 0.2 | 46 | 0.2 | 73 | 0.2 |
| 20 | 0.2 | 47 | 0.2 | 74 | 0.2 |
| 21 | 0.2 | 48 | 0.2 | 75 | 13.4 |
| 22 | 0.2 | 49 | 0.2 | 76 | 13.4 |
| 23 | 0.2 | 50 | 0.2 | 77 | 13.4 |
| 24 | 0.2 | 51 | 0.2 | 78 | 0.2 |
| 25 | 0.2 | 52 | 0.2 | 79 | 10.95 |
| 26 | 0.2 | 53 | 0.2 | ||
| 27 | 0.2 | 54 | 0.2 |
표 12에 나타낸 바와 같이, 상기 79개 효소는 3-HP-CoA를 AA-CoA로 전환하는 반응을 촉매하는 것을 확인하였다.
다음으로, 각 반응물에 대하여, (1)에 기재된 방법과 동일한 방법으로 반응물 및 생성물 (예, AA-CoA)의 피크 및 그 양을 확인하였다. HPLC 분석 결과, 표 2 내지 표 5에 열거된 79 효소에 대하여 AA-CoA가 생성되는 것이 확인되어, 3-HP-CoA를 AA-CoA로 전환하는 반응을 촉매하는 활성을 가진 것을 확인하였다.
(3)
AA
-
CoA
를
AA
로 전환하는 반응을
촉매하는
효소의 확인
먼저, 표 6에 열거된 AA-CoA를 AA로 전환하는 반응을 촉매하는 CoA hydrolase 유전자를 표 6에 언급된 미생물의 게놈을 주형으로 하고, HindIII 및 BamHI 제한 자리를 가진 각 프라이머 세트를 사용한 PCR을 통하여 증폭한 것을 제외하고, (1)에 기재된 방법과 동일하게 벡터를 제조하고, 대장균에 도입하고, 효소를 생산 및 정제하였다. 표 13은 사용된 프라이머 세트에 대한 정보를 나타낸다. 표 13에서 번호는 표 6에 나타낸 효소 또는 유전자 일련 번호를 나타낸다.
| 번호 | 서열번호:포워드/리버스 |
| 1 | 382/383 |
| 2 | 384/385 |
| 3 | 386/387 |
| 4 | 388/389 |
| 5 | 390/391 |
| 6 | 392/393 |
정제된 각 CoA hydrolase의 활성은 다음과 같이 분석하였다. 구체적으로, 100 mM Tris-HCl (pH 8.4), 2mM MgCl2, 3 mM ATP, 및 15 mM AA-CoA 함유 수용액, 상기 얻어진 각 효소 용액 (10 mg CoA hydrolase에 해당하는 양)를 첨가한 후, 약 2 내지 10분 동안 반응시켰다. 추정되는 반응식은 다음과 같다.
AA-CoA + CoA hydrolase -> AA + 유리된(free) CoA
다음으로, 각 반응물에 대하여, (1)에 기재된 방법과 동일하게 HPLC 분석을 수행하여, 반응물 및 생성물 (예, AA)의 피크 및 그 양을 확인하였다. 그 결과, 표 6에 언급된 6개 효소에 대하여 최종 산물 AA가 생성되는 것을 확인했다.
또한, 상기 각 반응물에 대하여, (1)에 기재된 방법과 동일한 방법을 사용하여 410nm에서 흡광도를 측정함으로써, 유리 CoA의 양을 확인하였다. 그 결과, 표 6에 열거된 6개 효소는 AA-CoA를 AA로 전환하는 반응을 촉매하는 활성을 갖는 것을 확인하였다. 표 14는 생성된 유리 CoA의 측정 결과를 나타낸다.
| 번호 | 생성된 CoA 양* |
| 1 | 0.7 |
| 2 | 0.2 |
| 3 | 0.06 |
| 4 | 0.06 |
| 5 | 0.1 |
| 6 | 0.2 |
*: 410nm에서의 흡광도 값을 나타내며, CoA hydrolase를 제외한 것을 제외하고는 동일하게 반응시킴 것을 대조군(control)으로 사용하였으며, 대조군은 410nm에서의 흡광도 값은 0이었다.
표 14에 나타낸 바와 같이, 표 6에 열거된 상기 6개 효소는 AA-CoA를 AA로 전환하는 반응을 촉매하는 것을 확인하였다.
실시예
2:
CoA
transferase
,
dehydratase
및
CoA
hydrolase
유전자가 도입 미생물 및 그를 이용한
AA
생산
본 실시예에서는 실시예 1에서 확인된 CoA transferase 유전자, dehydratase 유전자 및 CoA hydrolase 유전자 중 가장 활성이 높은 것으로 확인된, 대장균 유래의 CoA transferase (ydiF) 유전자, M.elsdenii 유래의 dehydratase MELS_1449 유전자, 및 대장균 유래의 CoA hydrolase yciA 유전자를 3-HP 생산능을 갖는 대장균에 도입하고, AA가 생성되는지를 확인하였다.
(1) 3-
HP
생산능을
갖는 대장균의 제조
(1.1)
ET
_
BAB
_
Dc5
벡터 제작
글리세롤로부터 3-히드록시프로피온산을 생산하는 미생물을 제조하기 위하여, ET_BAB_Dc5 벡터를 제작하였다.
Ilyobacter polytropus의 게놈 DNA로부터 글리세롤 데하이드라타제 (glycerol dehydratase: GDH)를 코딩하는 유전자 (dhaB1, dhaB2, dhaB3)(186, 187, 및 188) 및 글리세롤 데하이드라타제 재활성화효소 인자 (glycerol dehydratase reactivase: GDR)를 코딩하는 유전자 (gdrA, 및 gdrB)(서열번호 201, 및 202)를 확보하였다. 상기 dhaB1, dhaB2, 및 dhaB3 유전자는 Ilyobacter polytropus의 게놈 DNA를 주형으로 하고 dhaB123_F(서열번호 394) 및 dhaB123_R(서열번호 395)의 프라이머 세트를 사용한 PCR 증폭에 의하여 dhaB123를 한 증폭 산물로 얻었다. 상기 gdrA, 및 gdrB 유전자는 Ilyobacter polytropus의 게놈 DNA를 주형으로 하고 gdrAB_F(서열번호 396) 및 gdrAB_R(서열번호 397)의 프라이머 세트를 사용한 PCR 증폭에 의하여 gdrAB를 한 증폭 산물로 얻었다. 얻어진 PCR 산물을 BamHI 및 SacI 제한효소로 처리한 후, pETDuetTM-1 벡터 (Novagen)에 클로닝하였다.
또한, Cupriavidus necator의 게놈 DNA로부터 숙시네이트 세미알데히드 데히드로게나제 (succinate semialdehyde dehydrogenase: SSADH)를 코딩하는 유전자 (gabD, 서열번호 398)를 gabD_F (서열번호 399) 및 gabD_R (서열번호 400)의 프라이머 세트를 사용한 PCR 증폭에 의하여 얻었다. 얻어진 PCR 산물을 NdeI 및 KpnI 제한효소로 처리한 후, 상기 벡터에 클로닝하였다. 그 결과, pETDuet-1/dhaB_gdrAB_gabD 벡터를 얻었다.
도 1은 pETDuet/dhaB_gdrAB_gabD 벡터의 개열 지도를 나타낸다.
(1.2) 3-
HP
생산성 평가
(1)에서 제작된 벡터 pETDuet/dhaB_gdrAB_gabD4를 대장균 균주 (E.coli K(DE3))에 형질전환하였다.
배양은 250 ml 플라스크를 사용하여 50 ml 배지 (MgSO4 H2O 1.4 g/L, K2HPO4 17.4 g/L, KH2PO4 3 g/L, (NH4)2HPO4 4 g/L, 시트르산 1.7 g/L, ZnCl2 0.014 g/L, FeCl2 H2O 0.041 g/L, MnCl2 0.015 g/L, CuCl2 0.0015 g/L, H3BO3 0.003 g/L, Na2MoO4 0.0025 g/L, 니트릴로아세트산 200 mg/L, 소듐 셀레네이트 30 ㎍/L, 및 글리세롤 40 g/L)에서 33℃, 250 rpm으로 배양하였다. 배양 초기 600 nm에서의 흡광도(OD)가 0.8이 되었을 때 0.05 mM의 IPTG로 발현을 유도하였고 50 μM의 비타민 B12를 첨가하였다.
24시간 배양 후, 배양액 중 일부를 추출하여 흡광도 및 pH를 측정하고 HPLC (Waters)를 이용하여 3-HP 생산을 확인하였다. pH는 24시간마다 한 번씩 4N NaOH를 이용하여 pH 7.0으로 보정하였다. HPLC 분석에서 Aminex HPX-87H (300 mm × 7.8 mm) 컬럼을 사용하였고, 이동상은 9% 아세토니트릴이 함유된 0.5 mM 황산 용액을 사용하였다. 유속은 0.4 ml/분이고 컬럼의 온도는 35℃였으며, 검출기는 RI 및 UV/VIS (210 nm) 듀얼모드를 이용하였다. 3-HP는 총 35분의 분석 시간 중 17.5분에 검출되었다.
그 결과, 벡터 pETDuet/dhaB_gdrAB_gabD4를 대장균 균주 (E.coli K(DE3))에 혈질전환하여 얻어진 균주를 100mL 플라스크에서 24 시간 동안 상기와 동일한 조건에서 배양한 결과, 3-HP 생산량은 13.2g/L이었다.
(2)
AA
생산 균주의 제조
먼저, 실시예 1의 (1), (2) 및 (3)에 개시된 방법에 따라서, ydiF 유전자, MELS_1449 유전자 및 yciA 유전자를 증폭하고, 제한 효소를 사용하여 각각 소화시킨 후, 동일한 효소로 소화된 pETDuetTM-1 벡터에 각각 순차적으로 연결하여, 3개 유전자 발현용 벡터 (pETDuetTM-1/MELS_1449_yciA_YdiF)를 제조하였다. 이때 ydiF 유전자, MELS_1449 유전자 및 yciA 유전자 증폭에 사용된 프라이머 (서열번호 208/209, 236/237, 및 382/383)는 각각 BamHI과 HindIII, NdeI과 BglII, BglII와 XhoI 제한 자리를 가지고 있고, 벡터에 연결시 이 효소들을 각각 사용하여, 소화시켰다. 상기 발현용 벡터에서 각 효소는 벡터 중 His-Tag 코딩 서열과 작동가능하게 연결되어, 발현 산물은 His-tag이 융합된 형태로 발현되도록 한다.
도 2 및 도 3은 pETDuetTM-1/MELS_1449_yciA_YdiF의 지도 및 부분 확대된 도면이다.
다음으로, 상기 pETDuetTM-1/MELS_1449_yciA_YdiF 벡터를 3-HP 생산능이 있는 균주인 대장균 K(DE3)(pETDuet_dhaB_gdrAB_gabD4 )에 형질전환하였다. 이렇게 얻어진 pETDuetTM-1/MELS_1449_yciA_YdiF 벡터가 도입된 대장균을 M9 최소 배지 (MgSO4 H2O) 1.4 g/L, K2HPO4 17.4 g/L, KH2PO4 3g/L, (NH4)2HPO4 4 g/L, 시트르산 1.7 g/L, ZnCl2 0.014 g/L, FeCl2 H2O 0.041 g/L, MnCl2 0.015 g/L, CuCl2 0.0015 g/L, H3BO3 0.003 g/L, Na2MoO4 0.0025 g/L, 니트릴로아세트산 200 mg/L, 소듐 셀레네이트 30μg/L, 및 글리세롤 40 g/L) 100mL에서 OD600=0.1이 되게 접종하고 30℃에서 OD600=0.6이 될 때까지 배양한 후 0.02mM IPTG를 첨가하고 33℃에서 24 시간 동안 배양하였다. 배양은 250mL 플라스크에서 진탕하면서 24 시간 동안 이루어졌다.
다음으로, 배양물로부터 실시예 1의 (1), (2) 및 (3)에 기재된 바에 따라 발현된 MELS_1449, YdiF 및 YciA를 분리 및 정제하고, 이 세 가지 정제된 효소의 조합에 대하여 in vitro 효소 반응을 수행하고, 반응산물을 질량분석기(mass spectrometry)를 사용하여 분석하였다. 그 결과, 0.5g/L의 AA가 생산되었다.
다음으로, 배양물 중의 아크릴산 농도는 HPLC를 사용하여 측정하였다. 구체적으로, 배양이 종료된 후, 배양액 중 일부를 채취하여 흡광도를 측정하고, 세포가 제외된 배양액에 대하여 HPLC (Waters)를 사용하여 AA가 생산되는지를 확인하였다. HPLC 분석은 Aminex HPX-87H (300 mm x 7.8 mm) 칼럼을 사용하였고, 이동상은 9% 아세토니트릴이 함유된 0.5 mM 황산용액을 사용하였다. 유속은 0.4 ml/분이고 칼럼의 온도는 35℃였으며, 검출기는 RI 및 UV/VIS (210 nm) 듀얼모드를 이용하였다. HPLC 분석 결과, 상기 재조합 대장균 균주는 24 시간 동안 배양한 경우, 0.50 g/L의 아크릴산을 생산하였다.
도 4는 본 실시예의 대장균에서 포도당 또는 글리세롤로부터 아크릴산의 생산의 예측 경로를 나타낸 도면이다. 본 실시예에서 아크릴산은 도 4에 나타낸 경로에 따라 생산될 것으로 여겨지지만, 청구된 발명이 특별한 기작에 한정되는 것은 아니다.
<110> Samsung Electronics Co., Ltd.
<120> Microorganism having novel acrylic acid synthesis pathway and
method of producing acrylic acid using the same
<130> PN104850KR
<160> 404
<170> KopatentIn 2.0
<210> 1
<211> 524
<212> PRT
<213> Clostridium propionicum
<400> 1
Met Arg Lys Val Pro Ile Ile Thr Ala Asp Glu Ala Ala Lys Leu Ile
1 5 10 15
Lys Asp Gly Asp Thr Val Thr Thr Ser Gly Phe Val Gly Asn Ala Ile
20 25 30
Pro Glu Ala Leu Asp Arg Ala Val Glu Lys Arg Phe Leu Glu Thr Gly
35 40 45
Glu Pro Lys Asn Ile Thr Tyr Val Tyr Cys Gly Ser Gln Gly Asn Arg
50 55 60
Asp Gly Arg Gly Ala Glu His Phe Ala His Glu Gly Leu Leu Lys Arg
65 70 75 80
Tyr Ile Ala Gly His Trp Ala Thr Val Pro Ala Leu Gly Lys Met Ala
85 90 95
Met Glu Asn Lys Met Glu Ala Tyr Asn Val Ser Gln Gly Ala Leu Cys
100 105 110
His Leu Phe Arg Asp Ile Ala Ser His Lys Pro Gly Val Phe Thr Lys
115 120 125
Val Gly Ile Gly Thr Phe Ile Asp Pro Arg Asn Gly Gly Gly Lys Val
130 135 140
Asn Asp Ile Thr Lys Glu Asp Ile Val Glu Leu Val Glu Ile Lys Gly
145 150 155 160
Gln Glu Tyr Leu Phe Tyr Pro Ala Phe Pro Ile His Val Ala Leu Ile
165 170 175
Arg Gly Thr Tyr Ala Asp Glu Ser Gly Asn Ile Thr Phe Glu Lys Glu
180 185 190
Val Ala Pro Leu Glu Gly Thr Ser Val Cys Gln Ala Val Lys Asn Ser
195 200 205
Gly Gly Ile Val Val Val Gln Val Glu Arg Val Val Lys Ala Gly Thr
210 215 220
Leu Asp Pro Arg His Val Lys Val Pro Gly Ile Tyr Val Asp Tyr Val
225 230 235 240
Val Val Ala Asp Pro Glu Asp His Gln Gln Ser Leu Asp Cys Glu Tyr
245 250 255
Asp Pro Ala Leu Ser Gly Glu His Arg Arg Pro Glu Val Val Gly Glu
260 265 270
Pro Leu Pro Leu Ser Ala Lys Lys Val Ile Gly Arg Arg Gly Ala Ile
275 280 285
Glu Leu Glu Lys Asp Val Ala Val Asn Leu Gly Val Gly Ala Pro Glu
290 295 300
Tyr Val Ala Ser Val Ala Asp Glu Glu Gly Ile Val Asp Phe Met Thr
305 310 315 320
Leu Thr Ala Glu Ser Gly Ala Ile Gly Gly Val Pro Ala Gly Gly Val
325 330 335
Arg Phe Gly Ala Ser Tyr Asn Ala Asp Ala Leu Ile Asp Gln Gly Tyr
340 345 350
Gln Phe Asp Tyr Tyr Asp Gly Gly Gly Leu Asp Leu Cys Tyr Leu Gly
355 360 365
Leu Ala Glu Cys Asp Glu Lys Gly Asn Ile Asn Val Ser Arg Phe Gly
370 375 380
Pro Arg Ile Ala Gly Cys Gly Gly Phe Ile Asn Ile Thr Gln Asn Thr
385 390 395 400
Pro Lys Val Phe Phe Cys Gly Thr Phe Thr Ala Gly Gly Leu Lys Val
405 410 415
Lys Ile Glu Asp Gly Lys Val Ile Ile Val Gln Glu Gly Lys Gln Lys
420 425 430
Lys Phe Leu Lys Ala Val Glu Gln Ile Thr Phe Asn Gly Asp Val Ala
435 440 445
Leu Ala Asn Lys Gln Gln Val Thr Tyr Ile Thr Glu Arg Cys Val Phe
450 455 460
Leu Leu Lys Glu Asp Gly Leu His Leu Ser Glu Ile Ala Pro Gly Ile
465 470 475 480
Asp Leu Gln Thr Gln Ile Leu Asp Val Met Asp Phe Ala Pro Ile Ile
485 490 495
Asp Arg Asp Ala Asn Gly Gln Ile Lys Leu Met Asp Ala Ala Leu Phe
500 505 510
Ala Glu Gly Leu Met Gly Leu Lys Glu Met Lys Ser
515 520
<210> 2
<211> 531
<212> PRT
<213> Escherichia coli (strain K12)
<400> 2
Met Lys Pro Val Lys Pro Pro Arg Ile Asn Gly Arg Val Pro Val Leu
1 5 10 15
Ser Ala Gln Glu Ala Val Asn Tyr Ile Pro Asp Glu Ala Thr Leu Cys
20 25 30
Val Leu Gly Ala Gly Gly Gly Ile Leu Glu Ala Thr Thr Leu Ile Thr
35 40 45
Ala Leu Ala Asp Lys Tyr Lys Gln Thr Gln Thr Pro Arg Asn Leu Ser
50 55 60
Ile Ile Ser Pro Thr Gly Leu Gly Asp Arg Ala Asp Arg Gly Ile Ser
65 70 75 80
Pro Leu Ala Gln Glu Gly Leu Val Lys Trp Ala Leu Cys Gly His Trp
85 90 95
Gly Gln Ser Pro Arg Ile Ser Glu Leu Ala Glu Gln Asn Lys Ile Ile
100 105 110
Ala Tyr Asn Tyr Pro Gln Gly Val Leu Thr Gln Thr Leu Arg Ala Ala
115 120 125
Ala Ala His Gln Pro Gly Ile Ile Ser Asp Ile Gly Ile Gly Thr Phe
130 135 140
Val Asp Pro Arg Gln Gln Gly Gly Lys Leu Asn Glu Val Thr Lys Glu
145 150 155 160
Asp Leu Ile Lys Leu Val Glu Phe Asp Asn Lys Glu Tyr Leu Tyr Tyr
165 170 175
Lys Ala Ile Ala Pro Asp Ile Ala Phe Ile Arg Ala Thr Thr Cys Asp
180 185 190
Ser Glu Gly Tyr Ala Thr Phe Glu Asp Glu Val Met Tyr Leu Asp Ala
195 200 205
Leu Val Ile Ala Gln Ala Val His Asn Asn Gly Gly Ile Val Met Met
210 215 220
Gln Val Gln Lys Met Val Lys Lys Ala Thr Leu His Pro Lys Ser Val
225 230 235 240
Arg Ile Pro Gly Tyr Leu Val Asp Ile Val Val Val Asp Pro Asp Gln
245 250 255
Thr Gln Leu Tyr Gly Gly Ala Pro Val Asn Arg Phe Ile Ser Gly Asp
260 265 270
Phe Thr Leu Asp Asp Ser Thr Lys Leu Ser Leu Pro Leu Asn Gln Arg
275 280 285
Lys Leu Val Ala Arg Arg Ala Leu Phe Glu Met Arg Lys Gly Ala Val
290 295 300
Gly Asn Val Gly Val Gly Ile Ala Asp Gly Ile Gly Leu Val Ala Arg
305 310 315 320
Glu Glu Gly Cys Ala Asp Asp Phe Ile Leu Thr Val Glu Thr Gly Pro
325 330 335
Ile Gly Gly Ile Thr Ser Gln Gly Ile Ala Phe Gly Ala Asn Val Asn
340 345 350
Thr Arg Ala Ile Leu Asp Met Thr Ser Gln Phe Asp Phe Tyr His Gly
355 360 365
Gly Gly Leu Asp Val Cys Tyr Leu Ser Phe Ala Glu Val Asp Gln His
370 375 380
Gly Asn Val Gly Val His Lys Phe Asn Gly Lys Ile Met Gly Thr Gly
385 390 395 400
Gly Phe Ile Asp Ile Ser Ala Thr Ser Lys Lys Ile Ile Phe Cys Gly
405 410 415
Thr Leu Thr Ala Gly Ser Leu Lys Thr Glu Ile Thr Asp Gly Lys Leu
420 425 430
Asn Ile Val Gln Glu Gly Arg Val Lys Lys Phe Ile Arg Glu Leu Pro
435 440 445
Glu Ile Thr Phe Ser Gly Lys Ile Ala Leu Glu Arg Gly Leu Asp Val
450 455 460
Arg Tyr Ile Thr Glu Arg Ala Val Phe Thr Leu Lys Glu Asp Gly Leu
465 470 475 480
His Leu Ile Glu Ile Ala Pro Gly Val Asp Leu Gln Lys Asp Ile Leu
485 490 495
Asp Lys Met Asp Phe Thr Pro Val Ile Ser Pro Glu Leu Lys Leu Met
500 505 510
Asp Glu Arg Leu Phe Ile Asp Ala Ala Met Gly Phe Val Leu Pro Glu
515 520 525
Ala Ala His
530
<210> 3
<211> 542
<212> PRT
<213> Cupriavidus necator
<400> 3
Met Lys Val Ile Thr Ala Arg Glu Ala Ala Ala Leu Val Gln Asp Gly
1 5 10 15
Trp Thr Val Ala Ser Ala Gly Phe Val Gly Ala Gly His Ala Glu Ala
20 25 30
Val Thr Glu Ala Leu Glu Gln Arg Phe Leu Gln Ser Gly Leu Pro Arg
35 40 45
Asp Leu Thr Leu Val Tyr Ser Ala Gly Gln Gly Asp Arg Gly Ala Arg
50 55 60
Gly Val Asn His Phe Gly Asn Ala Gly Met Thr Ala Ser Ile Val Gly
65 70 75 80
Gly His Trp Arg Ser Ala Thr Arg Leu Ala Thr Leu Ala Met Ala Glu
85 90 95
Gln Cys Glu Gly Tyr Asn Leu Pro Gln Gly Val Leu Thr His Leu Tyr
100 105 110
Arg Ala Ile Ala Gly Gly Lys Pro Gly Val Met Thr Lys Ile Gly Leu
115 120 125
His Thr Phe Val Asp Pro Arg Thr Ala Gln Asp Ala Arg Tyr His Gly
130 135 140
Gly Ala Val Asn Glu Arg Ala Arg Gln Ala Ile Ala Gly Gly Lys Ala
145 150 155 160
Cys Trp Val Asp Ala Val Asp Phe Arg Gly Glu Glu Tyr Leu Phe Tyr
165 170 175
Pro Ser Phe Pro Ile His Cys Ala Leu Ile Arg Cys Thr Ala Ala Asp
180 185 190
Thr Arg Gly Asn Leu Ser Thr His Arg Glu Ala Phe His His Glu Leu
195 200 205
Leu Ala Met Ala Gln Ala Ala His Asn Ser Gly Gly Ile Val Ile Ala
210 215 220
Gln Val Glu Ser Leu Val Asp His His Glu Ile Leu Gln Ala Ile His
225 230 235 240
Val Pro Gly Ile Leu Val Asp Tyr Val Val Val Cys Asp Asn Pro Ala
245 250 255
Asn His Gln Met Thr Phe Ala Glu Ser Tyr Asn Pro Ala Tyr Val Thr
260 265 270
Pro Trp Gln Gly Glu Ala Ala Val Val Glu Ala Glu Ala Thr Pro Val
275 280 285
Ala Ala Gly Pro Leu Asp Ala Arg Thr Ile Val Gln Arg Arg Ala Val
290 295 300
Met Glu Leu Ala Arg Arg Ala Pro Arg Val Val Asn Leu Gly Val Gly
305 310 315 320
Met Pro Ala Ala Val Gly Met Leu Ala His Gln Ala Gly Leu Asp Gly
325 330 335
Phe Thr Leu Thr Val Glu Ala Gly Pro Ile Gly Gly Thr Pro Ala Asp
340 345 350
Gly Leu Ser Phe Gly Ala Ser Ala Tyr Pro Glu Ala Val Val Asp Gln
355 360 365
Pro Ala Gln Phe Asp Phe Tyr Glu Gly Gly Gly Ile Asp Leu Ala Ile
370 375 380
Leu Gly Leu Ala Glu Leu Asp Gly His Gly Asn Val Asn Val Ser Lys
385 390 395 400
Phe Gly Glu Gly Glu Gly Ala Ser Ile Ala Gly Val Gly Gly Phe Ile
405 410 415
Asn Ile Thr Gln Ser Ala Arg Ala Val Val Phe Met Gly Thr Leu Thr
420 425 430
Ala Gly Gly Leu Glu Val Arg Ala Gly Glu Gly Arg Leu Gln Ile Val
435 440 445
Arg Glu Gly Arg Val Lys Lys Ile Val Pro Glu Val Ser His Leu Ser
450 455 460
Phe Asn Gly Pro Tyr Val Ala Ser Leu Gly Ile Pro Val Leu Tyr Ile
465 470 475 480
Thr Glu Arg Ala Val Phe Glu Met Arg Ala Gly Ala Gly Gly Glu Ala
485 490 495
Arg Leu Thr Leu Val Glu Ile Ala Pro Gly Val Asp Leu Gln Arg Asp
500 505 510
Val Leu Asp Gln Cys Ala Thr Pro Val Ala Val Ala Pro Asp Leu Arg
515 520 525
Glu Met Asp Ala Arg Leu Phe Gln Ala Gly Pro Leu His Leu
530 535 540
<210> 4
<211> 473
<212> PRT
<213> Halomonas smyrnensis
<400> 4
Met Thr Thr Ser Thr Gln Thr Glu Leu Lys Lys Gly Pro Leu Asn Gly
1 5 10 15
Ile Thr Val Leu Asp Phe Ser Arg Val Leu Ala Gly Pro Tyr Cys Thr
20 25 30
Met Val Leu Ala Asp Leu Gly Ala Arg Val Ile Lys Ile Glu Lys Ile
35 40 45
Gly Thr Gly Asp Asp Thr Arg Glu Phe Gly Pro Phe Val Glu Gly Glu
50 55 60
Ser Ala Tyr Phe Ser Cys Phe Asn Arg Asn Lys Glu Ser Ile Val Leu
65 70 75 80
Asp Ile Lys Ser Pro Arg Asp Arg Glu Leu Leu Glu Arg Leu Leu Asp
85 90 95
Glu Ser Asp Val Leu Val Glu Asn Phe Arg Pro Gly Val Met Asp Arg
100 105 110
Leu Gly Tyr Gly Pro Glu Arg Leu Ala Met Thr His Pro His Leu Ile
115 120 125
Tyr Ser Ser Ile Ser Gly Phe Gly His Thr Gly Pro Phe Ser Glu Leu
130 135 140
Pro Gly Tyr Asp Met Val Val Gln Ala Met Gly Gly Ile Met Ser Leu
145 150 155 160
Thr Gly Trp Pro Asp Gly Glu Pro Ala Arg Val Gly Thr Ser Phe Gly
165 170 175
Asp Leu Ser Ala Ala Leu Phe Ala Ala Ile Gly Ile Ile Ala Ser Leu
180 185 190
Tyr Ser Arg Thr Lys Asp Ala Gln Gly Thr Arg Val Asp Ile Gly Met
195 200 205
Leu Asp Cys Gln Ala Ala Leu Met Glu Thr Ala Leu Ala Arg Tyr Asp
210 215 220
Ile Glu Gly Lys Val Pro Asn Arg Thr Gly Asp Cys His Pro Ser Leu
225 230 235 240
Ala Pro Phe Glu Ser Phe Asn Ala Lys Asp Gly Lys Leu Val Ile Ala
245 250 255
Ala Gly Asn Asp Thr Leu Phe Leu Leu Met Ala Asp Ala Ile Gly Ala
260 265 270
Ala Arg Leu Ala Phe Asp Pro Arg Phe Ile Ser Asn Asp Leu Arg Cys
275 280 285
His Asn Arg Pro Glu Leu Val Ala Glu Met Glu Lys Ile Leu Leu Glu
290 295 300
Gln Pro Val Gln His Trp Val Asp Leu Leu Asn Glu Glu Gly Val Pro
305 310 315 320
Cys Ser Pro Ile Asn Thr Ile Asp Lys Leu Phe Asp His Pro Gln Leu
325 330 335
Lys Ala Arg Asn Met Ile Val Gln Val Gln Gly Lys Ala Gly Lys Pro
340 345 350
Phe Lys Thr Ala Gly Asn Pro Ile Lys Leu Thr Gly Arg Glu Asp Phe
355 360 365
Asp Ser Gly Val Pro Met Ser Ala Pro Gly Leu Gly Gln His Arg Glu
370 375 380
Ala Ile Leu Thr Glu Leu Met Ala Arg His Gly Ser Tyr His Leu Pro
385 390 395 400
Thr Gln Pro Asp Ile Gln Gly Ser Leu Pro Glu Ser Gly Thr Asp Thr
405 410 415
Pro Trp Ala Phe Arg Pro Val Ala Ser Gly Asp Ser Gly Ala Ala Leu
420 425 430
Ser Arg Asn Glu Pro Lys Ser Lys Asn Ala Gln Val Ala Ser Glu Asn
435 440 445
Glu Arg Gly Arg Ser Ser Asn Ser Glu Pro Gln Pro Lys Ser Thr Ala
450 455 460
Lys Ser Lys Ser Glu Met Glu Asn Gly
465 470
<210> 5
<211> 629
<212> PRT
<213> Ruegeria pomeroyi DSS-3
<400> 5
Met Ala Tyr Gln Asp Val Tyr Glu Ser Trp Lys Ser Asp Pro Glu Ala
1 5 10 15
Phe Trp Met Glu Ala Ala Lys Ser Ile Asp Trp Val Glu Ala Pro Ser
20 25 30
Arg Ala Leu Asp Asp Ser Asn Ala Pro Leu Tyr Glu Trp Phe Thr Asp
35 40 45
Ala Lys Val Asn Thr Cys Trp Asn Ala Val Asp Arg His Val Glu Ala
50 55 60
Gly Arg Gly Glu Gln Thr Ala Ile Ile Tyr Asp Ser Pro Ile Thr His
65 70 75 80
Thr Lys Arg Gln Ile Ser Tyr Val Glu Leu Arg Asn Arg Val Ala Met
85 90 95
Leu Ala Gly Ala Leu Arg Ala Lys Gly Val Glu Lys Gly Asp Arg Val
100 105 110
Ile Ile Tyr Met Pro Met Ile Pro Glu Ala Leu Glu Ala Met Leu Ala
115 120 125
Cys Ala Arg Leu Gly Ala Val His Ser Val Val Phe Gly Gly Phe Ala
130 135 140
Ala Asn Glu Leu Ala Val Arg Ile Asp Asp Ala Gln Pro Lys Ala Ile
145 150 155 160
Ile Ala Ala Ser Cys Gly Leu Glu Pro Gly Arg Val Val His Tyr Lys
165 170 175
Pro Leu Leu Asp Gly Ala Ile Asp Met Ala Lys His Lys Pro Glu Phe
180 185 190
Cys Val Ile Phe Gln Arg Glu Gln Glu Val Ala His Leu Glu Glu Gly
195 200 205
Arg Asp Tyr Asn Trp His Gly Phe Gln Tyr Gly Val Glu Pro Ala Glu
210 215 220
Cys Val Pro Val Glu Gly Asn His Pro Ala Tyr Ile Leu Tyr Thr Ser
225 230 235 240
Gly Thr Thr Gly Ala Pro Lys Gly Val Leu Arg Pro Thr Ala Gly His
245 250 255
Leu Val Ala Leu Asn Trp Thr Met Lys Asn Ile Tyr Asn Val Asp Pro
260 265 270
Gly Asp Val Phe Trp Ala Ala Ser Asp Val Gly Trp Val Val Gly His
275 280 285
Ser Tyr Ile Cys Tyr Gly Pro Leu Ile His Gly Asn Thr Thr Ile Val
290 295 300
Phe Glu Gly Lys Pro Val Gly Thr Pro Asp Ala Gly Thr Phe Trp Arg
305 310 315 320
Val Ile Ser Glu His Lys Val Lys Ser Phe Phe Thr Ala Pro Thr Ala
325 330 335
Ile Arg Ala Val Lys Arg Glu Asp Pro Lys Gly Glu Met Leu Ala Lys
340 345 350
Tyr Asp Leu Ser His Leu Lys Ala Leu Tyr Leu Ala Gly Glu Arg Ala
355 360 365
Asp Pro Asp Thr Ile Ile Trp Ala Gln Lys Ala Leu Ser Val Pro Val
370 375 380
Ile Asp His Trp Trp Gln Thr Glu Thr Gly Trp Thr Ile Ala Gly Asn
385 390 395 400
Pro Leu Gly Ile Glu Glu Leu Pro Thr Lys Leu Gly Ser Pro Ala Lys
405 410 415
Ala Met Pro Gly Tyr Asp Val Gln Ile Leu Asp Glu Gly Gly His Gln
420 425 430
Met Lys Pro Gly Glu Leu Gly Ala Ile Ala Val Lys Leu Pro Leu Pro
435 440 445
Pro Gly Thr Leu Pro Gly Leu Trp Asn Ala Glu Ala Arg Phe Arg Lys
450 455 460
Ser Tyr Leu Glu His Phe Pro Gly Tyr Tyr Glu Thr Gly Asp Ala Gly
465 470 475 480
Met Ile Asp Glu Asp Gly Tyr Leu Tyr Ile Met Ala Arg Thr Asp Asp
485 490 495
Val Ile Asn Val Ala Gly His Arg Leu Ser Thr Gly Gly Met Glu Glu
500 505 510
Val Leu Ala Gly His Glu Asp Val Ala Glu Cys Ala Val Ile Gly Val
515 520 525
Ser Asp Asp Leu Lys Gly Gln Met Pro Leu Gly Phe Leu Cys Leu Asn
530 535 540
Asn Gly Cys Asn Arg Asp His Gly Asp Val Val Lys Glu Val Val Lys
545 550 555 560
Leu Val Arg Asp Lys Ile Gly Pro Val Ala Ala Phe Lys Leu Ala Val
565 570 575
Val Val Asp Arg Leu Pro Lys Thr Arg Ser Gly Lys Ile Leu Arg Gly
580 585 590
Thr Met Val Ser Ile Ala Asp Gly Lys Glu Tyr Lys Met Pro Ala Thr
595 600 605
Ile Asp Asp Pro Ala Ile Leu Asp Glu Ile Lys Val Ala Leu Gln Ser
610 615 620
Leu Gly Tyr Ala Lys
625
<210> 6
<211> 518
<212> PRT
<213> Desulfosporosinus youngiae DSM 17734
<400> 6
Met Lys Lys Val Ile Val Leu Thr Ala Glu Glu Ala Val Asp Leu Val
1 5 10 15
Lys Asp Gly Asp Thr Leu Cys Thr Ser Gly Phe Val Gly Asn Ser Leu
20 25 30
Pro Glu Ala Leu Phe Lys Ala Ile Glu Lys Lys Phe Leu Gly Thr Gly
35 40 45
Tyr Pro Gln Asn Ile Thr Leu Phe Tyr Pro Ala Ser Gln Gly Ser Arg
50 55 60
Asn Gly Thr Gly Gly Asp His Phe Ala His Glu Gly Leu Val Lys Arg
65 70 75 80
Val Ile Ala Gly His Leu Asn Thr Ala Pro Lys Leu Gly Glu Leu Cys
85 90 95
Leu Ala Asn Lys Cys Glu Gly Tyr Asn Leu Pro Gln Gly Ala Leu Glu
100 105 110
Tyr Val Ile Arg Asp Ala Ala Gly His Arg Pro Gly Thr Ile Thr His
115 120 125
Val Gly Leu Gly Thr Phe Val Asp Pro Arg Asn Gly Gly Gly Lys Ile
130 135 140
Asn Ala Arg Thr Thr Glu Asp Leu Val Glu Val Ile Lys Ile Gly Asn
145 150 155 160
Glu Glu Lys Leu Phe Tyr Lys Ala Phe Pro Ile Asp Ile Ala Phe Leu
165 170 175
Arg Gly Thr Tyr Ala Asp Glu Tyr Gly Asn Val Thr Leu Glu Lys Glu
180 185 190
Val Ala Thr Ile Glu Val Thr Ser Ile Ala Gln Ala Val Ile Asn Asn
195 200 205
Gly Gly Lys Val Ile Val Gln Val Glu Lys Val Val Lys Gly Gly Thr
210 215 220
Leu Asp Pro Arg Leu Val Gln Ile Pro Gly Ile Tyr Val His Ala Val
225 230 235 240
Val Glu Val Ala Asp Met Lys Asp His Glu Gln Ser Val Gly His Glu
245 250 255
Tyr Asn Pro Ala Leu Cys Gly Glu Ala Arg Ala Pro Glu Gly Gly Val
260 265 270
Glu Thr Thr Pro Leu Ser Ile Lys Lys Val Ile Gly Arg Arg Ala Ala
275 280 285
Met Glu Leu Val Glu Asn Thr Val Val Asn Leu Gly Val Gly Thr Pro
290 295 300
Glu Tyr Val Ala Gln Val Ala Ser Glu Glu Gly Ile Ala Ser Tyr Met
305 310 315 320
Thr Leu Thr Val Glu Ser Gly Ala Ile Gly Gly Ser Pro Gln Gly Gly
325 330 335
Ala Arg Phe Gly Ala Thr Leu Asn Pro Asp Ala Ile Ile Asp Gln Asn
340 345 350
Ser Gln Phe Asp Phe Tyr Asp Gly Gly Gly Leu Asp Met Ala Phe Leu
355 360 365
Gly Leu Ala Glu Cys Asp Lys Gln Gly Asn Ile Asn Val Ser Lys Phe
370 375 380
Gly Pro Lys Ile Pro Gly Cys Gly Gly Phe Ile Asn Ile Thr Gln Asn
385 390 395 400
Ala Lys Lys Val Phe Phe Cys Gly Thr Phe Thr Ala Gly Gly Leu Lys
405 410 415
Glu Arg Ile Glu Asp Gly Lys Leu Ile Val Glu Gln Glu Gly Arg Gln
420 425 430
Arg Lys Phe Ile Lys Glu Val Glu Gln Val Thr Phe Ser Gly Asn Tyr
435 440 445
Ala Val Lys Thr Lys Gln Pro Val Leu Tyr Ile Thr Glu Arg Ala Val
450 455 460
Phe Glu Leu Lys Glu Asp Gly Leu Asn Leu Ile Glu Val Ala Pro Gly
465 470 475 480
Ile Asp Ile Gln Thr Gln Ile Ile Asp Leu Met Asp Phe Ala Pro Lys
485 490 495
Ile Asp Ser Gly Leu Lys Leu Met Asp Ala Arg Leu Phe Ser Glu Glu
500 505 510
Leu Met Asn Leu Lys Lys
515
<210> 7
<211> 123
<212> PRT
<213> Peptoniphilus indolicus ATCC 29427
<400> 7
Met Leu Glu Lys Gly Ser Thr Gln Glu Leu Thr Glu Ile Val Thr Lys
1 5 10 15
Glu Asn Thr Ala Asp Ala Val Gly Asn Lys Ser Val Phe Val Tyr Ala
20 25 30
Thr Pro Phe Leu Val Ala Leu Met Glu Arg Thr Cys Ile Lys Leu Met
35 40 45
Glu Glu Asp Leu Asp Ser Gly Glu Val Ser Val Gly Thr Asn Ile Asn
50 55 60
Leu Asp His Leu Ala Pro Thr Pro Ile Gly Asn Lys Ile Leu Cys Arg
65 70 75 80
Ala Glu Leu Leu Glu Gln Ser Gly Lys Lys Tyr Val Phe Asp Val Lys
85 90 95
Ala Phe Asp Asn Asp Lys Leu Ile Gly Lys Ala Ile His Thr Arg Tyr
100 105 110
Lys Val Asn Leu Asp Lys Phe Leu Asn Asn Ile
115 120
<210> 8
<211> 518
<212> PRT
<213> Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10)
<400> 8
Met Lys Lys Val Asn Ile Leu Thr Ala Glu Glu Ala Val Asp Leu Val
1 5 10 15
Lys Asp Gly Asp Thr Leu Cys Thr Ser Gly Phe Val Gly Asn Ser Leu
20 25 30
Pro Glu Ala Leu Phe Lys Ala Ile Glu Lys Lys Phe Leu Glu Thr Gly
35 40 45
Tyr Pro Gln Asn Ile Thr Leu Phe Tyr Pro Ala Ser Gln Gly Ser Arg
50 55 60
Asn Gly Thr Gly Gly Asp His Phe Ala His Glu Gly Leu Val Lys Arg
65 70 75 80
Val Ile Ala Gly His Leu Asn Thr Ala Pro Lys Leu Gly Glu Leu Cys
85 90 95
Leu Ala Asn Lys Cys Glu Gly Tyr Asn Leu Pro Gln Gly Ala Leu Glu
100 105 110
Tyr Val Ile Arg Asp Ala Ala Gly His Arg Pro Gly Thr Ile Thr His
115 120 125
Val Gly Leu Gly Thr Phe Val Asp Pro Arg Asn Gly Gly Gly Lys Ile
130 135 140
Asn Ala Arg Thr Thr Glu Asp Leu Val Glu Val Ile Lys Ile Gly Asn
145 150 155 160
Glu Glu Lys Leu Phe Tyr Lys Ala Phe Pro Ile Asp Ile Ala Phe Leu
165 170 175
Arg Gly Thr Tyr Ala Asp Glu Tyr Gly Asn Val Thr Leu Glu Lys Glu
180 185 190
Val Ala Thr Ile Glu Val Thr Ser Ile Ala Gln Ala Val Ile Asn Asn
195 200 205
Gly Gly Lys Val Ile Val Gln Val Glu Lys Val Val Lys Gly Gly Thr
210 215 220
Leu Asp Pro Arg Leu Val Gln Ile Pro Gly Ile Tyr Val His Gly Val
225 230 235 240
Val Glu Val Val Asp Met Lys Asp His Glu Gln Ser Val Gly His Glu
245 250 255
Tyr Asn Pro Ala Leu Cys Gly Ala Val Arg Ala Pro Glu Gly Asn Val
260 265 270
Glu Lys Thr Pro Leu Ser Ile Lys Lys Val Ile Gly Arg Arg Ala Ala
275 280 285
Met Glu Leu Val Lys Asp Thr Val Val Asn Leu Gly Val Gly Thr Pro
290 295 300
Glu Tyr Val Ala Gln Val Ala Ser Glu Glu Glu Ile Ala Ser Tyr Met
305 310 315 320
Thr Leu Thr Val Glu Ser Gly Ala Ile Gly Gly Ser Pro Gln Gly Gly
325 330 335
Ala Arg Phe Gly Ala Thr Leu Asn Pro Asp Ala Ile Ile Asp Gln Asn
340 345 350
Ser Gln Phe Asp Phe Tyr Asp Gly Gly Gly Leu Asp Met Ala Phe Leu
355 360 365
Gly Leu Ala Glu Cys Asp Glu Gln Gly Asn Ile Asn Val Ser Arg Phe
370 375 380
Gly Pro Lys Ile Pro Gly Cys Gly Gly Phe Ile Asn Ile Thr Gln Asn
385 390 395 400
Ala Lys Lys Val Phe Phe Cys Gly Thr Phe Thr Ala Arg Gly Leu Lys
405 410 415
Gln Lys Ile Glu Asp Gly Lys Leu Ile Ile Glu Gln Glu Gly Thr Gln
420 425 430
Arg Lys Phe Ile Lys Gln Val Glu Gln Val Thr Phe Ser Gly Lys Tyr
435 440 445
Ala Ile Lys Thr Lys Gln Pro Val Leu Tyr Ile Thr Glu Arg Ala Val
450 455 460
Phe Glu Leu Lys Glu Glu Gly Leu Asn Leu Ile Glu Val Ala Pro Gly
465 470 475 480
Ile Asp Ile Gln Thr Gln Ile Ile Asp Leu Met Asp Phe Val Pro Thr
485 490 495
Val Asp Lys Asn Ile Lys Ile Met Asp Ser Arg Leu Phe Ser Glu Glu
500 505 510
Leu Met Asn Leu Lys Lys
515
<210> 9
<211> 521
<212> PRT
<213> Desulfosporosinus orientis (strain ATCC 19365/DSM 765/NCIMB 8382/VKM B-1628)
<400> 9
Met Arg Lys Val Lys Ile Met Thr Ala Glu Glu Ala Val Asp Leu Val
1 5 10 15
Lys Asp Gly Val Thr Leu Cys Thr Ser Gly Phe Val Gly Asn Ser Leu
20 25 30
Pro Glu Ala Leu Tyr Lys Ala Ile Glu Lys Lys Phe Leu Glu Thr Gly
35 40 45
Tyr Pro Gln Ser Ile Thr Leu Phe Tyr Pro Ala Ser Gln Gly Ser Arg
50 55 60
Asn Gly Thr Gly Gly Asp His Phe Ala His Glu Gly Leu Val Lys Arg
65 70 75 80
Val Ile Ala Gly His Leu Asn Thr Ala Pro Lys Leu Gly Glu Leu Cys
85 90 95
Leu Ala Asn Lys Cys Glu Gly Tyr Asn Leu Pro Gln Gly Thr Leu Glu
100 105 110
Tyr Val Ile Arg Asp Ala Ala Gly His Arg Pro Gly Thr Ile Thr His
115 120 125
Val Gly Leu Gly Thr Phe Val Asp Pro Arg Asn Gly Gly Gly Lys Ile
130 135 140
Asn Ala Arg Thr Thr Glu Asn Leu Val Glu Val Val Lys Ile Gly Asn
145 150 155 160
Glu Glu Lys Leu Phe Tyr Lys Ala Phe Pro Ile Asp Ile Ala Phe Leu
165 170 175
Arg Gly Thr Tyr Ala Asp Glu Tyr Gly Asn Val Thr Leu Glu Lys Glu
180 185 190
Val Ala Thr Ile Glu Val Thr Ser Ile Ala Gln Ala Val Ile Asn Asn
195 200 205
Gly Gly Lys Val Ile Val Gln Val Glu Lys Val Val Lys Gly Gly Thr
210 215 220
Leu Asp Pro Arg Leu Val Gln Ile Pro Gly Ile Tyr Val His Ala Val
225 230 235 240
Val Glu Ala Asp Leu Lys Asp His Glu Gln Ser Val Gly His Glu Tyr
245 250 255
Asn Pro Ala Leu Cys Gly Glu Ala Arg Ala Pro Glu Gly Asn Val Glu
260 265 270
Ile Thr Pro Leu Ser Ile Lys Lys Val Ile Gly Arg Arg Ala Ala Met
275 280 285
Glu Leu Val Glu Asp Thr Val Val Asn Leu Gly Val Gly Thr Pro Glu
290 295 300
Tyr Val Ala Gln Val Ala Ser Glu Glu Gly Ile Ala Asn Tyr Met Thr
305 310 315 320
Leu Thr Val Glu Ser Gly Ala Ile Gly Gly Ser Pro Gln Gly Gly Ala
325 330 335
Arg Phe Gly Ala Thr Leu Asn Pro Asp Ala Ile Ile Asp Gln Asn Ser
340 345 350
Gln Phe Asp Phe Tyr Asp Gly Gly Gly Leu Asp Met Ala Phe Leu Gly
355 360 365
Leu Ala Glu Cys Asp Glu Gln Gly Asn Ile Asn Val Ser Arg Phe Gly
370 375 380
Pro Lys Ile Pro Gly Cys Gly Gly Phe Ile Asn Ile Thr Gln Asn Ala
385 390 395 400
Lys Lys Val Phe Phe Cys Gly Thr Phe Thr Ala Gly Gly Leu Lys Glu
405 410 415
Arg Ile Glu Asp Gly Met Leu Ile Ile Asp Gln Glu Gly Lys Gln Arg
420 425 430
Lys Phe Ile Lys Glu Val Glu Gln Val Thr Phe Ser Gly Arg Tyr Ala
435 440 445
Ile Lys Thr Glu Gln Pro Val Leu Tyr Ile Thr Glu Arg Ala Val Phe
450 455 460
Glu Leu Lys Glu Glu Gly Leu Asn Leu Ile Glu Ile Ala Pro Gly Val
465 470 475 480
Asp Ile Gln Thr Gln Ile Ile Asp Leu Met Asp Phe Val Pro Thr Ile
485 490 495
Asp Lys Asn Leu Lys Leu Met Asp Pro Arg Leu Phe Ser Glu Glu Lys
500 505 510
Met Asn Leu Lys Asn Asn Glu Gly Glu
515 520
<210> 10
<211> 412
<212> PRT
<213> Peptostreptococcus anaerobius CAG:621
<400> 10
Met Ser Thr Arg Lys Arg Pro Leu Glu Gly Val Lys Val Ile Glu Leu
1 5 10 15
Ala Asn Phe Ile Ala Ala Ala Thr Thr Gly Arg Phe Leu Ala Asp Leu
20 25 30
Gly Ala Asp Val Ile Lys Ile Glu Ser Ala Lys Gly Asp Pro Leu Arg
35 40 45
Tyr Thr Ala Pro Thr Glu Gly Arg Pro Leu Asp Met His Glu Asn Thr
50 55 60
Thr Trp Glu Leu Glu Asn Ala Asn Lys Arg Cys Ile Ser Leu Asn Met
65 70 75 80
Lys Asp Pro Lys Gly Lys Glu Ala Phe Phe Lys Leu Leu Asp Asp Ala
85 90 95
Asp Ile Leu Ile Thr Asn Trp Arg Val Gln Ala Leu Gln Arg Ala Gly
100 105 110
Leu Asp Tyr Glu Thr Leu Lys Val Lys Tyr Pro Ser Leu Val Tyr Ala
115 120 125
Ile Cys Thr Gly Tyr Gly Glu Tyr Gly Pro Asp Lys Asp Leu Pro Gly
130 135 140
Phe Asp Phe Thr Ala Phe Phe Ala Arg Gly Gly Tyr Leu Glu Asn Leu
145 150 155 160
Arg Gln Lys Ser Asp Val Pro Met Asn Val Val Pro Gly Leu Gly Asp
165 170 175
His Asn Val Gly Ile Asn Leu Ala Ala Gly Val Leu Ala Ala Leu Tyr
180 185 190
His Ala Lys Leu Thr Gly Glu Gly Glu Lys Val Glu Thr Ser Leu Phe
195 200 205
Glu Ser Ala Ile Phe Asn Met Gly Met Met Val Gln Ala Ala Gln Tyr
210 215 220
Pro Asp Tyr Gly Thr Pro Tyr Pro Ile Asn Ile Arg Glu Ala Asn Asn
225 230 235 240
Pro Phe Asn Ala Cys Trp Met Thr Lys Asp Gly Arg Tyr Val Gln Thr
245 250 255
Cys Met Pro Asp Tyr Asn Thr Tyr Phe Lys Lys Phe Leu Thr Ala Leu
260 265 270
Glu Leu Met Asp Ile Val Glu Asp Glu Arg Phe Phe Pro Val Gln Asn
275 280 285
Leu His Ala Asn Asp Leu Gly Thr Thr Val Tyr Asp Arg Val Met Ala
290 295 300
Arg Phe Gly Glu Arg Asp Phe Ser Glu Trp Ser Gln Val Leu Thr Asp
305 310 315 320
Ala Asp Ile Pro Phe Ala Leu Ala Lys Asn Trp Glu Glu Leu Leu Glu
325 330 335
Asp Glu Gln Ala Trp Ala Asn Asp Cys Phe Tyr Lys Met Lys Tyr Pro
340 345 350
Ser Gly Glu Arg Ile Leu Cys Lys His Pro Val Lys Tyr His Glu Met
355 360 365
Gly Pro Thr Pro Tyr Asn Arg Gly Pro Tyr Ile Gly Glu His Gly Val
370 375 380
Glu Val Met Lys Glu Leu Gly Tyr Ser Asp Glu Asp Ile Lys Gln Met
385 390 395 400
Leu Glu Asp Lys Thr Leu Tyr Val Trp Glu Asp Lys
405 410
<210> 11
<211> 1575
<212> DNA
<213> Clostridium propionicum
<400> 11
atgagaaagg ttcccattat taccgcagat gaggctgcaa agcttattaa agacggtgat 60
acagttacaa caagtggttt cgttggaaat gcaatccctg aggctcttga tagagctgta 120
gaaaaaagat tcttagaaac aggcgaaccc aaaaacatta catatgttta ttgtggttct 180
caaggtaaca gagacggaag aggtgctgag cactttgctc atgaaggcct tttaaaacgt 240
tacatcgctg gtcactgggc tacagttcct gctttgggta aaatggctat ggaaaataaa 300
atggaagcat ataatgtatc tcagggtgca ttgtgtcatt tgttccgtga tatagcttct 360
cataagccag gcgtatttac aaaggtaggt atcggtactt tcattgaccc cagaaatggc 420
ggcggtaaag taaatgatat taccaaagaa gatattgttg aattggtaga gattaagggt 480
caggaatatt tattctaccc tgcttttcct attcatgtag ctcttattcg tggtacttac 540
gctgatgaaa gcggaaatat cacatttgag aaagaagttg ctcctctgga aggaacttca 600
gtatgccagg ctgttaaaaa cagtggcggt atcgttgtag ttcaggttga aagagtagta 660
aaagctggta ctcttgaccc tcgtcatgta aaagttccag gaatttatgt tgactatgtt 720
gttgttgctg acccagaaga tcatcagcaa tctttagatt gtgaatatga tcctgcatta 780
tcaggcgagc atagaagacc tgaagttgtt ggagaaccac ttcctttgag tgcaaagaaa 840
gttattggtc gtcgtggtgc cattgaatta gaaaaagatg ttgctgtaaa tttaggtgtt 900
ggtgcgcctg aatatgtagc aagtgttgct gatgaagaag gtatcgttga ttttatgact 960
ttaactgctg aaagtggtgc tattggtggt gttcctgctg gtggcgttcg ctttggtgct 1020
tcttataatg cggatgcatt gatcgatcaa ggttatcaat tcgattacta tgatggcggc 1080
ggcttagacc tttgctattt aggcttagct gaatgcgatg aaaaaggcaa tatcaacgtt 1140
tcaagatttg gccctcgtat cgctggttgt ggtggtttca tcaacattac acagaataca 1200
cctaaggtat tcttctgtgg tactttcaca gcaggtggct taaaggttaa aattgaagat 1260
ggcaaggtta ttattgttca agaaggcaag cagaaaaaat tcttgaaagc tgttgagcag 1320
attacattca atggtgacgt tgcacttgct aataagcaac aagtaactta tattacagaa 1380
agatgcgtat tccttttgaa ggaagatggt ttgcacttat ctgaaattgc acctggtatt 1440
gatttgcaga cacagattct tgacgttatg gattttgcac ctattattga cagagatgca 1500
aacggccaaa tcaaattgat ggacgctgct ttgtttgcag aaggcttaat gggtctgaag 1560
gaaatgaagt cctga 1575
<210> 12
<211> 1596
<212> DNA
<213> Escherichia coli (strain K12)
<400> 12
atgaaacctg taaaaccacc tcgtattaat ggacgagtgc cggtcctgtc ggcacaggaa 60
gcggtgaatt atattcccga cgaagcaaca ctttgtgtgt taggcgctgg cggcggtatt 120
ctggaagcca ccacgttaat tactgctctt gctgataaat ataaacagac tcaaacacca 180
cgtaatttat cgattattag tccaacaggg cttggcgatc gcgccgaccg tggtattagt 240
cctctggcgc aagaaggtct ggtgaaatgg gcattatgtg gtcactgggg acaatcgccg 300
cgtatttctg aactcgcaga acaaaataaa attattgctt ataactaccc acaaggtgta 360
cttacacaaa ccttacgcgc cgccgcagcc caccagcctg gtattattag tgatattggc 420
atcgggacat ttgtcgatcc acgccagcaa ggcggcaaac tgaatgaagt cactaaagaa 480
gacctgatta aactggtcga gtttgataac aaagaatatc tctattacaa agcgattgcg 540
ccagatattg ccttcattcg cgctaccacc tgcgacagtg aaggctacgc cacttttgaa 600
gatgaggtga tgtatctcga cgcattggtt attgcccagg cggtgcacaa taacggcggt 660
attgtgatga tgcaggtgca gaaaatggtt aagaaagcca cgctgcatcc taaatctgtc 720
cgtattccgg gttatctggt ggatattgtg gtggtcgatc cggatcaaac ccaactgtat 780
ggcggtgcac cggttaaccg ctttatttct ggtgacttca cccttgatga cagtaccaaa 840
cttagcctgc ccctaaacca acgtaaatta gttgcgcggc gcgcattatt cgaaatgcgt 900
aaaggcgcgg tggggaatgt cggcgtcggt attgctgacg gcattggcct ggtcgcccga 960
gaagaaggtt gtgctgatga ctttattctg acggtagaaa caggtccgat tggcggaatt 1020
acttcacagg ggatcgcctt tggcgcgaac gtgaataccc gtgccattct ggatatgacg 1080
tcccagtttg atttttatca cggtggcggt ctggatgttt gttatttgag ttttgctgaa 1140
gtcgaccagc acggtaacgt cggcgtgcat aaattcaatg gtaaaatcat gggcaccggt 1200
ggatttattg atatcagtgc cacttcgaag aaaatcattt tctgcggcac attaactgcg 1260
ggcagtttaa aaacagaaat taccgacggc aaattaaata tcgtccagga aggacgggtg 1320
aagaaattta ttcgggaact accggaaatt actttcagcg gaaaaatcgc tctcgagcga 1380
gggctggatg ttcgttatat cactgagcgc gcagtattca cgctgaaaga agacggcctg 1440
catttaatcg aaatcgcccc tggcgtcgat ttacaaaaag atattctcga caaaatggat 1500
ttcaccccag tgatttcgcc agaactcaaa ctgatggacg aaagattatt tatcgatgcg 1560
gcgatgggtt ttgtcctgcc tgaagcggct cattaa 1596
<210> 13
<211> 1629
<212> DNA
<213> Cupriavidus necator
<400> 13
atgaaggtga tcaccgcacg cgaagcggcg gcactggtgc aggacggctg gaccgtggcc 60
agcgcgggct ttgtcggcgc cggccatgcc gaggccgtga ccgaggcgct ggagcagcgc 120
ttcctgcaga gcgggctgcc gcgcgacctg acgctggtgt actcggccgg gcagggcgac 180
cgcggcgcgc gcggcgtgaa ccacttcggc aatgccggca tgaccgccag catcgtcggc 240
ggccactggc gctcggccac gcggctggcc acgctggcca tggccgagca gtgcgagggc 300
tacaacctgc cgcagggcgt gctgacgcac ctataccgcg ccatcgccgg cggcaagccc 360
ggcgtgatga ccaagatcgg cctgcacacc ttcgtcgacc cgcgcaccgc gcaggatgcg 420
cgctaccacg gcggcgccgt caacgagcgc gcgcgccagg ccattgccga gggcaaggca 480
tgctgggtcg atgcggtcga cttccgcggc gacgaatacc tgttctaccc gagcttcccg 540
atccactgcg cgctgatccg ctgcaccgcg gccgacgccc gcggcaacct cagcacccat 600
cgcgaagcct tccaccatga gctgctggcg atggcgcagg cggcccacaa ctcgggcggc 660
atcgtgatcg cgcaggtgga aagcctggtc gaccaccacg agatcctgca ggccatccac 720
gtgcccggca tcctggtcga ctacgtggtg gtctgcgaca accccgccaa ccaccagatg 780
acgtttgccg agtcctacaa cccggcctac gtgacgccat ggcaaggcga ggcagcggtg 840
gccgaagcgg aagcggcgcc ggtggctgcc ggcccgctcg acgcgcgcac catcgtgcag 900
cgccgtgcgg tgatggaact ggcgcgccgt gcgccgcgcg tggtcaacct gggcgtgggc 960
atgccggcag cggtcggcat gctggcgcac caggccgggc tggacggctt cacgctgacc 1020
gtcgaggccg gccccatcgg cggcacgccc gcggatggcc tcagcttcgg tgcctcggcc 1080
tacccggagg cggtggtgga tcagcccgcg cagttcgatt tctacgaggg cggcggcatc 1140
gacctggcca tcctcggcct ggccgagctg gatggccacg gcaacgtcaa tgtcagcaag 1200
ttcggcgaag gcgagggcgc atcgattgcc ggcgtcggcg gctttatcaa catcacgcag 1260
agcgcgcgcg cggtggtgtt catgggcacg ctgacggcgg gcgggctgga agtccgcgcc 1320
ggcgacggcg gcctgcagat cgtgcgcgaa ggccgcgtga agaagatcgt gcctgaggtg 1380
tcgcacctga gcttcaacgg gccctatgtg gcgtcgctcg gcatcccggt gctgtacatc 1440
accgagcgcg cggtgttcga gatgcgcgct ggcgcagacg gcgaagcccg cctcacgctg 1500
gtcgagatcg cccccggcgt ggacctgcag cgcgacgtgc tcgaccagtg ctcgacgccc 1560
atcgccgttg cgcaggacct gcgcgaaatg gatgcgcggc tgttccaggc cgggcccctg 1620
cacctgtaa 1629
<210> 14
<211> 1421
<212> DNA
<213> Halomonas smyrnensis
<400> 14
atgaccacct caacgcaaac ggaacttaag aagggccctc tcaacgggat taccgtgctt 60
gatttttctc gggtactcgc cgggccctat tgcaccatgg tgctggccga tttaggcgcc 120
cgtgtcatta agattgaaaa gatcggaacc ggcgatgaca cgcgggagtt tggaccgttc 180
gtcgagggcg agagcgcgta tttcagctgc ttcaacagga acaaggaaag cattgtcctg 240
gatattaagt cgccgcgcga ccgcgagctg ctggaacgat tactggacga gtctgatgtg 300
ctagtcgaaa actttcggcc tggagtgatg gaccgtctgg ggtatggccc ggaacgcttg 360
gccatgacgc accctcactt aatctactca tcgatctctg gcttcggtca taccggcccc 420
ttcagtgagt tacccggcta cgacatggtg gtgcaggcca tggggggaat tatgagcctg 480
acggggtggc ccgatggtga gccggctcgc gtgggaacca gcttcggtga cctgagtgcg 540
gcactgttcg cggcgatcgg tatcatcgca tcgctctata gccgaaccaa ggatgctcag 600
ggaacccgtg tcgatatcgg catgctcgac tgccaggcgg cactgatgga aaccgcattg 660
gctcgctatg acatcgaggg gaaagtgccg aaccgaaccg gcgattgcca cccctcactg 720
gcgcccttcg aaagcttcaa tgccaaggac ggcaagctcg tcatcgccgc tggcaacgat 780
acccttttcc tattaatggc cgatgccatc ggtgcggcaa ggctcgcctt cgatcctcgg 840
tttatcagta acgacttgcg ttgtcacaac cggcccgagt tggtcgctga aatggaaaag 900
atcctactgg agcagcccgt gcagcactgg gtggatctgc tgaacgagga gggggtgcct 960
tgttcgccga tcaacaccat cgacaagcta ttcgatcatc cgcagctgaa agctcgaaat 1020
atgatcgttc aggttcaggg taaggccggt aagcctttca aaacagcggg taatccgatc 1080
aagctgaccg ggcgcgagga ctttgatagt ggcgtcccaa tgagtgcgcc gggtcttggg 1140
caacatcgag aagccatctt gacggagttg atggctcgtc atggttccta tcatttgcca 1200
acacagccgg acattcaggg ctcgttaccg gaatctggaa cggacactcc atgggcgttt 1260
cgccctgtcg catccggtga ttcaggggcc gctctttcga gaaacgagcc gaagagtaag 1320
aacgcccaag tagcgtctga aaacgagcgg ggccgatcgt caaactctga gccacaaccc 1380
aagtcgaccg ccaaaagtaa atcggagatg gaaaatggat a 1421
<210> 15
<211> 1889
<212> DNA
<213> Ruegeria pomeroyi DSS-3
<400> 15
atggcatatc aggacgtata cgagagctgg aagagcgatc ccgaagcctt ctggatggag 60
gccgccaaaa gcatcgactg ggttgaagcg cccagccgcg cgctggatga cagcaacgcg 120
ccgctttacg aatggttcac cgatgccaag gtcaacacct gctggaacgc cgttgaccgc 180
catgtcgagg cgggtcgggg cgagcagacc gcgatcatct atgacagccc gatcacccat 240
accaagcggc agatctctta cgtcgagctg cgcaaccggg tggccatgct ggccggcgcg 300
ctgcgcgcca aaggtgtgga aaagggcgat cgcgtcatca tctatatgcc gatgatcccc 360
gaggcgctgg aggcgatgct ggcctgtgcc cgtctgggcg ccgtgcattc ggtggtcttc 420
ggcgggtttg ccgccaacga actggccgta cgcatcgacg acgcccaacc caaggcgatc 480
atcgcggcat cctgcgggtt ggaaccgggc cgcgtggtgc attacaagcc cctcctcgac 540
ggcgccatcg acatggcgaa acacaagccc gaattctgtg tgatcttcca gcgcgaacag 600
gaagtggcgc atctcgaaga gggccgcgat tacaattggc acgggttcca gtatggcgtc 660
gaaccggccg aatgcgtccc cgtagagggc aaccacccgg cctatatcct ctatacctcc 720
ggcaccactg gcgcgcccaa gggggtgctg cgtccgaccg cgggtcatct ggtggcgctg 780
aactggacga tgaagaacat ctataacgtc gatccgggcg atgtgttctg ggcggcatcc 840
gatgtgggct gggtcgtcgg ccacagctat atctgctatg gcccactgat ccacggcaac 900
accaccatcg tattcgaggg caagcccgtc ggcacccccg atgcgggcac cttctggcgg 960
gtgatctccg agcacaaggt caagagcttc ttcaccgccc ccaccgccat ccgcgccgtg 1020
aaacgcgagg atcccaaggg cgagatgctg gcgaaatacg acctgagcca tctcaaggcg 1080
ctctatctgg cgggcgaacg ggccgatccc gacaccatca tctgggcgca aaaggcgctg 1140
agtgtgcctg tgatcgacca ttggtggcag accgaaaccg gctggaccat cgcgggcaac 1200
ccgctgggga tagaggagct gccgacaaaa ctgggctcgc ccgccaaggc aatgcccggc 1260
tatgacgtac agatcctcga cgagggcggc caccagatga aaccgggcga gctgggcgcc 1320
atcgcggtga aactgccgct gccgccgggc accctgcccg gcctgtggaa cgccgaggcg 1380
cggttcagga aatcctatct ggagcatttc cccggctact atgagaccgg cgacgccggc 1440
atgatcgacg aggacgggta cctctatatc atggcgcgca ccgatgacgt catcaacgtt 1500
gcgggccacc gcctgtccac cggcggcatg gaagaggttc tggccgggca cgaggatgtg 1560
gccgaatgcg cggtgatcgg cgtcagcgac gacctcaagg gtcagatgcc gctgggcttc 1620
ctgtgcctaa acaacgggtg caaccgcgac cacggggatg tggtgaaaga ggtagtcaag 1680
ctggtgcgcg acaagatcgg cccagtcgcg gccttcaagc tggccgtggt ggtcgaccgc 1740
ctgcccaaga cccgctcggg caagatcctg cgcggcacca tggtctcgat cgctgacggc 1800
aaggagtaca agatgcccgc caccatcgac gatccggcca tcctggacga gatcaaggtc 1860
gcactgcaat cgctgggcta cgccaagta 1889
<210> 16
<211> 1557
<212> DNA
<213> Desulfosporosinus youngiae DSM 17734
<400> 16
atgaagaagg ttatcgttct gacagcagaa gaggctgtag atttagttaa agatggggac 60
actctttgta caagtggatt tgttggaaat agtcttccgg aagcactttt taaggctata 120
gaaaagaaat ttttagggac cggctatcct caaaatataa cgttgtttta tccggcatca 180
caaggcagca ggaacggcac aggcggtgat cattttgctc acgaaggttt ggttaaaaga 240
gtcatagcag gtcatttaaa tactgctccg aagttaggcg aactgtgtct ggctaataag 300
tgtgaaggtt ataacttacc acaaggtgct ttggaatatg ttatcagaga tgctgcgggt 360
cataggcccg gcacaattac ccatgttggg ttaggtacgt ttgttgatcc gagaaatggc 420
ggaggtaaaa taaacgccag gacgacagaa gatttagtag aagttataaa aattggcaat 480
gaagaaaagc ttttctacaa agcatttcct atagatatcg ctttcctgag aggtacttat 540
gcggatgagt atggcaatgt aaccctggaa aaagaagtag ctactattga ggttacatct 600
atagcacagg cagttatcaa taatggcgga aaagtcattg ttcaggtgga aaaggttgta 660
aaaggcggta ctttagaccc cagactggtt caaatacctg gcatttatgt tcatgctgtt 720
gtagaagtag ctgatatgaa agatcacgaa caaagcgttg gacatgaata taacccggca 780
ctttgcgggg aagcaagagc acctgaaggt ggcgttgaaa cgaccccatt aagtattaaa 840
aaggttattg gcagaagagc agctatggaa ttggtagaga ataccgttgt taatttaggt 900
gtaggaacac ctgagtatgt tgctcaagtt gccagtgaag aaggaatagc aagttatatg 960
accttaacag ttgaatcagg ggcaatagga ggcagtcctc aaggcggagc cagatttggt 1020
gctactttaa atcctgacgc tattattgat caaaatagtc agtttgattt ttatgatggc 1080
ggcggtctgg acatggcatt tttaggctta gctgaatgtg ataaacaggg caacattaat 1140
gtcagcaaat ttggccccaa aataccaggt tgcggtggtt tcatcaatat cacccaaaat 1200
gccaaaaaag tattcttctg cggcacattt acagccgggg gcttaaaaga aagaattgaa 1260
gatggcaagc taatagtcga gcaagaaggc aggcaaagga aatttattaa agaagttgaa 1320
caagttacat ttagtggaaa ttatgccgtt aaaacgaaac aaccggtttt atatattaca 1380
gaaagagctg tatttgaatt aaaagaagat ggacttaact taatagaagt tgctcctgga 1440
attgatatcc aaacccaaat tatcgattta atggattttg cccctaagat tgacagcgga 1500
cttaagctca tggacgcaag actttttagt gaagaactaa tgaacttgaa aaaataa 1557
<210> 17
<211> 372
<212> DNA
<213> Peptoniphilus indolicus ATCC 29427
<400> 17
atgctggaaa aaggtagtac acaagaactt accgaaattg ttacaaaaga aaatacagct 60
gatgctgtag gaaataaatc agtatttgtt tatgcaactc catttttagt agcattaatg 120
gagcgaactt gtataaaatt gatggaagaa gatttagaca gtggagaagt ttcagttgga 180
actaatataa atttagatca tcttgctcca acaccaattg gaaataaaat tttatgcaga 240
gcagaactgc ttgaacaaag tggtaaaaaa tatgtatttg atgtaaaagc ttttgataat 300
gacaaactta ttggaaaggc aatacataca agatacaaag ttaacttaga taaattttta 360
aataacattt aa 372
<210> 18
<211> 1557
<212> DNA
<213> Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10)
<400> 18
atgaagaagg ttaatattct cacagcagaa gaggctgttg atttagtcaa agatggggac 60
actctttgta ccagtggatt tgttggcaat agtcttccgg aagcactttt taaagctata 120
gaaaagaaat ttttagagac tggctatcct caaaatataa ctttgtttta tccagcatca 180
caaggcagca gaaacggcac gggcggtgat cattttgctc acgaaggttt ggttaaaagg 240
gtcatagcag gtcatttaaa tactgctccg aagttaggcg aactgtgtct agctaataaa 300
tgcgaaggtt ataacttacc acaaggtgct ttggaatatg ttattagaga tgctgcgggt 360
cataggcccg gtacaattac ccatgttgga ttaggtactt ttgttgatcc aagaaatggc 420
ggaggtaaaa taaacgctag gacgacagaa gatttagtag aagttataaa aatcggcaat 480
gaagaaaagc ttttctacaa agcttttcct atagatatcg ccttccttag aggtacttat 540
gcggatgagt atggtaatgt aaccctagaa aaagaagtag ctactattga ggttacatct 600
atagcacagg cagttataaa taatggtgga aaagtaattg ttcaagtgga aaaggttgta 660
aaaggtggta ctttagaccc cagactggta caaataccag gcatttatgt tcatggtgtt 720
gtagaagtag ttgatatgaa agatcatgaa caaagtgttg gacatgaata taatccagca 780
ctttgcgggg cagtaagagc acctgaaggt aatgttgaaa aaaccccatt gagtattaaa 840
aaagttattg gcagaagagc agctatggaa ttggtaaagg atacagttgt taatttaggt 900
gttggaacac ctgagtatgt tgctcaagtt gcaagtgaag aagagatagc tagttatatg 960
actttaactg ttgaatcagg agcaatagga ggcagtcctc aaggtggagc cagatttggt 1020
gctactttaa atcctgacgc tattattgac caaaatagtc agtttgattt ttatgatggc 1080
ggcggtctgg atatggcatt tttaggttta gctgaatgtg atgaacaggg caacattaat 1140
gtcagcagat tcggtcccaa aataccggga tgtggtggtt tcatcaatat cacccaaaat 1200
gcaaaaaaag tattcttctg cggtacattt acagcaagag gcttaaaaca aaaaattgaa 1260
gatggcaagt taataattga acaagaagga acgcaaagga aatttattaa acaagttgaa 1320
caggtaacat ttagtggaaa atatgcaatt aaaactaaac aacctgtttt atatataaca 1380
gaaagagctg tatttgaatt aaaagaagag ggacttaact taattgaagt tgctcccgga 1440
attgatattc aaacacaaat tatagattta atggattttg tccctacagt tgacaaaaat 1500
attaaaatta tggactcaag actttttagt gaagaactaa tgaacttgaa gaaataa 1557
<210> 19
<211> 1566
<212> DNA
<213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628)
<400> 19
atgaggaagg ttaagattat gacagcagaa gaggctgttg atttagttaa agatggggtg 60
actctctgta caagtggatt tgttggaaat agtcttccgg aagcacttta taaggctatc 120
gaaaagaaat ttttagagac cggctatcct caaagtatca ctttatttta tccggcctca 180
caaggcagca gaaatggcac cggcggtgat cattttgctc acgaaggttt ggttaaaaga 240
gtcatagccg gtcatttaaa tactgctccc aagttaggcg agctgtgtct ggctaataaa 300
tgtgaaggtt ataacttacc ccaaggtact ttggaatatg ttatcagaga tgctgcgggt 360
cataggcccg gtaccatcac ccatgtaggc ttaggtactt ttgttgatcc cagaaatggc 420
ggaggtaaaa taaacgccag gacgacagaa aatttagtgg aagttgtaaa aatcggcaat 480
gaagaaaagc ttttctacaa agcatttcct atagatatcg ctttcttgag aggtacttat 540
gcggatgagt atggtaatgt aaccctggaa aaagaagtag ctactattga ggttacttcc 600
atagcacagg cagttatcaa taatggcggc aaagtaattg ttcaagtgga aaaggttgta 660
aaaggcggga ctttagaccc cagactggtg caaatacctg gcatttatgt tcatgctgtt 720
gtcgaagctg atctgaaaga tcatgaacaa agtgtcgggc atgaatataa cccggcactg 780
tgcggggaag caagggcacc tgaaggcaat gttgaaataa ctcccttaag tattaaaaaa 840
gttattggca gaagagccgc tatggaattg gtagaggata ccgttgtcaa tttaggtgtt 900
ggaacacctg agtatgttgc tcaagttgcc agtgaagaag gaatcgccaa ttatatgacc 960
ttaaccgtag aatccggtgc catcggcggc agtcctcaag ggggagcccg atttggtgct 1020
actttaaatc ctgacgctat tattgatcaa aatagtcagt ttgactttta cgatggcggc 1080
ggtctggaca tggcattttt aggcttagct gaatgtgatg aacagggcaa cattaacgtc 1140
agccgctttg gtcccaaaat acccggttgc ggcggtttca tcaatatcac ccagaatgcc 1200
aaaaaagtat tcttctgcgg tacatttact gcaggaggct taaaagaaag aattgaagac 1260
ggcatgctaa tcatagatca agagggaaaa cagcggaaat ttatcaaaga agttgaacag 1320
gttaccttta gcggaagata tgccattaaa accgaacagc ccgttttata tattacggaa 1380
agagctgtct ttgaattaaa agaagagggg ctgaacttaa tcgaaatcgc tcccggcgtt 1440
gatatccaaa cccaaattat tgatttaatg gattttgtcc ctaccattga caaaaacctc 1500
aagcttatgg acccaagact tttcagtgag gaaaaaatga acttgaaaaa caacgaggga 1560
gaataa 1566
<210> 20
<211> 1239
<212> DNA
<213> Peptostreptococcus anaerobius CAG:621
<400> 20
atgagcacaa gaaagagacc attagaagga gtaaaggtaa tagaacttgc aaactttata 60
gcagctgcaa ctacaggaag atttttagct gacttaggtg cagatgtaat aaagattgaa 120
agtgcaaagg gagacccact aagatatacg gctccaactg aaggtagacc gcttgatatg 180
cacgaaaata caacttggga attagaaaat gctaataaga gatgtatatc tctaaatatg 240
aaggatccta agggtaaaga agctttcttt aagctacttg atgatgcaga tatacttata 300
acaaactgga gagtacaggc actacagaga gctggacttg actatgaaac actaaaggtt 360
aagtatccaa gccttgtata cgctatttgt acaggttatg gtgaatacgg tcctgataag 420
gatcttccag gattcgactt tacagctttc tttgctagag gtggatatct tgaaaacctt 480
agacagaaat ctgatgttcc aatgaacgtt gttccaggac ttggtgacca caatgttggt 540
atcaacctag ctgcaggtgt acttgctgca ttatatcacg ctaaattaac aggcgaaggt 600
gaaaaggttg aaacaagttt attcgaatca gcaatattca atatgggtat gatggttcag 660
gctgctcagt atcctgacta cggtactcca tatccaataa atataagaga agctaacaat 720
ccatttaacg cttgttggat gactaaggac ggaagatatg ttcagacttg tatgcctgac 780
tacaatactt acttcaaaaa gttcttaaca gcattagagc ttatggatat agtagaagat 840
gaaagattct tcccagtgca gaacttacat gcaaacgacc taggaactac agtatatgat 900
agagtaatgg ctaggtttgg agaaagagac tttagcgaat ggtcacaggt acttacagat 960
gctgacatcc catttgctct agctaagaat tgggaagaac tattagaaga tgaacaggca 1020
tgggcaaatg actgcttcta caagatgaag tatccaagtg gtgaaagaat actttgtaag 1080
cacccagtta agtaccatga aatgggacct actccataca acagaggacc atacatcggt 1140
gaacatggtg ttgaagtaat gaaggagtta ggatactctg atgaagacat caagcagatg 1200
ttagaagaca agactctata tgtttgggaa gacaaatag 1239
<210> 21
<211> 299
<212> PRT
<213> Dictyostelium discoideum (Slime mold)
<400> 21
Met Ile Asn Arg Leu Phe Ser Ile Asn Asn Ile Lys Asn Gly Ser Lys
1 5 10 15
Phe Phe Ser Ser Ser Thr Thr Val Glu Thr Lys Gln Pro Leu Val Leu
20 25 30
Leu Glu Lys His Leu Val Asn Gly Lys Tyr Thr Gly Ile Gln Ile Val
35 40 45
Lys Leu Asn Lys Pro Lys Gln Leu Asn Ala Leu Thr Phe Glu Met Gly
50 55 60
Val Asp Tyr Lys Lys Val Val Asp Thr Leu Ala Glu Asp Lys Asp Leu
65 70 75 80
Lys Cys Val Val Leu Thr Gly Glu Gly Lys Ala Phe Ser Ala Gly Gly
85 90 95
Asp Leu Asp Phe Leu Ile Glu Arg Thr Lys Asp Thr Pro Glu Asn Asn
100 105 110
Gln Arg Ile Met Glu Arg Phe Tyr Arg Thr Phe Leu Tyr Ile Arg Ser
115 120 125
Leu Pro Val Pro Ile Ile Ser Ala Ile Asn Gly Ala Ala Ile Gly Ala
130 135 140
Gly Phe Cys Leu Ala Leu Ala Thr Asp Ile Arg Val Val Ser Asn Lys
145 150 155 160
Ala Pro Val Gly Leu Thr Phe Thr Lys Leu Gly Ile His Pro Gly Met
165 170 175
Gly Val Thr His Ser Ile Thr Asn Ile Val Gly Gln Asp Val Ala Ser
180 185 190
Tyr Met Leu Leu Ser Ser Asp Ile Ile Lys Gly Asp Glu Ala Gln Arg
195 200 205
Leu Gly Leu Val Leu Lys Ser Val Glu Ser Asp Gln Val Leu Pro Thr
210 215 220
Ala Leu Asn Leu Ala Glu Thr Ile Ser Lys Asn Ser Thr Ile Ala Val
225 230 235 240
Asn Ser Thr Thr Lys Thr Leu Arg Asn Lys Tyr Asn Ser Asp Leu Asp
245 250 255
Lys Ser Leu Thr Arg Glu Ala Asp Ala Gln Ser Gln Cys Trp Ala Ser
260 265 270
Lys Asp Ile Val Glu Gly Ile Leu Ala Ile Arg Glu Ser Arg Asp Pro
275 280 285
Lys His Asn Tyr Leu Leu Phe Asp Asp Gln Lys
290 295
<210> 22
<211> 261
<212> PRT
<213> Clostridium acetobutylicum
<400> 22
Met Glu Leu Asn Asn Val Ile Leu Glu Lys Glu Gly Lys Val Ala Val
1 5 10 15
Val Thr Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ser Asp Thr
20 25 30
Leu Lys Glu Met Asp Tyr Val Ile Gly Glu Ile Glu Asn Asp Ser Glu
35 40 45
Val Leu Ala Val Ile Leu Thr Gly Ala Gly Glu Lys Ser Phe Val Ala
50 55 60
Gly Ala Asp Ile Ser Glu Met Lys Glu Met Asn Thr Ile Glu Gly Arg
65 70 75 80
Lys Phe Gly Ile Leu Gly Asn Lys Val Phe Arg Arg Leu Glu Leu Leu
85 90 95
Glu Lys Pro Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly
100 105 110
Cys Glu Ile Ala Met Ser Cys Asp Ile Arg Ile Ala Ser Ser Asn Ala
115 120 125
Arg Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly
130 135 140
Gly Thr Gln Arg Leu Ser Arg Leu Val Gly Met Gly Met Ala Lys Gln
145 150 155 160
Leu Ile Phe Thr Ala Gln Asn Ile Lys Ala Asp Glu Ala Leu Arg Ile
165 170 175
Gly Leu Val Asn Lys Val Val Glu Pro Ser Glu Leu Met Asn Thr Ala
180 185 190
Lys Glu Ile Ala Asn Lys Ile Val Ser Asn Ala Pro Val Ala Val Lys
195 200 205
Leu Ser Lys Gln Ala Ile Asn Arg Gly Met Gln Cys Asp Ile Asp Thr
210 215 220
Ala Leu Ala Phe Glu Ser Glu Ala Phe Gly Glu Cys Phe Ser Thr Glu
225 230 235 240
Asp Gln Lys Asp Ala Met Thr Ala Phe Ile Glu Lys Arg Lys Ile Glu
245 250 255
Gly Phe Lys Asn Arg
260
<210> 23
<211> 155
<212> PRT
<213> Clostridium difficile
<400> 23
Asn Ser Lys Lys Val Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly
1 5 10 15
Gly Cys Glu Leu Ala Met Ala Cys Asp Ile Arg Ile Ala Ser Ala Lys
20 25 30
Ala Lys Phe Gly Gln Pro Glu Val Thr Leu Gly Ile Thr Pro Gly Tyr
35 40 45
Gly Gly Thr Gln Arg Leu Thr Arg Leu Val Gly Met Ala Lys Ala Lys
50 55 60
Glu Leu Ile Phe Thr Gly Gln Val Ile Lys Ala Asp Glu Ala Glu Lys
65 70 75 80
Ile Gly Leu Val Asn Arg Val Val Glu Pro Asp Ile Leu Ile Glu Glu
85 90 95
Val Glu Lys Leu Ala Lys Ile Ile Ala Lys Asn Ala Gln Leu Ala Val
100 105 110
Arg Tyr Ser Lys Glu Ala Ile Gln Leu Gly Ala Gln Thr Asp Ile Asn
115 120 125
Thr Gly Ile Asp Ile Glu Ser Asn Leu Phe Gly Leu Cys Phe Ser Thr
130 135 140
Lys Asp Gln Lys Glu Gly Ile Val Ser Phe Arg
145 150 155
<210> 24
<211> 258
<212> PRT
<213> Clostridium pasteurianum
<400> 24
Met Gly Asn Ile Ile Phe Glu Glu Glu Asp Gly Ile Glu Lys Val Thr
1 5 10 15
Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ser Glu Thr Leu Lys
20 25 30
Glu Leu Gly Thr Val Ile Asn Asp Ile Ser Val Asn Asp Gly Ile Lys
35 40 45
Ala Val Ile Ile Thr Gly Ser Gly Ser Lys Ala Phe Val Ala Gly Ala
50 55 60
Asp Ile Ala Glu Met Ser Thr Leu Asn Ser Ile Glu Ala Thr Asn Phe
65 70 75 80
Ser Arg Leu Ala Gln Asn Val Phe Ser Gln Ile Glu Asn Leu Pro Lys
85 90 95
Leu Val Val Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly Cys Glu
100 105 110
Leu Ala Met Ala Cys Asp Val Arg Phe Ala Ser Lys Lys Ala Lys Phe
115 120 125
Gly Gln Pro Glu Val Asn Leu Gly Ile Leu Pro Ser Phe Gly Gly Thr
130 135 140
Gln Arg Leu Pro Lys Leu Val Gly Lys Gly Ile Ala Lys Glu Leu Ile
145 150 155 160
Phe Ser Thr Asp Met Ile Thr Ala Asp Glu Ala Tyr Arg Ile Gly Leu
165 170 175
Ala Asn Lys Val Tyr Glu Pro Glu Glu Leu Leu Val Lys Ser Gln Glu
180 185 190
Phe Ala Glu Lys Val Met Thr Lys Ser Pro Trp Gly Val Lys Leu Ala
195 200 205
Lys Ala Cys Ile Asn Asn Gly Leu Asp Val Asp Leu Glu Ala Gly Leu
210 215 220
Lys Tyr Glu Ala Asn Ser Phe Gly Leu Cys Phe Ser Thr Glu Asp Gln
225 230 235 240
Lys Glu Gly Met Lys Ala Phe Leu Glu Lys Arg Lys Ala Asp Phe Lys
245 250 255
Gly Leu
<210> 25
<211> 262
<212> PRT
<213> Clostridium pasteurianum
<400> 25
Met Asp Phe Asn Asn Ile Ile Leu Glu Lys Glu Glu Lys Ile Ala Val
1 5 10 15
Val Thr Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ser Glu Thr
20 25 30
Leu Thr Glu Leu Asp Ser Val Ile Asp Glu Ile Asp Lys Asp Asn Glu
35 40 45
Ile Leu Ala Val Val Leu Thr Gly Ala Gly Lys Ser Phe Val Ala Gly
50 55 60
Ala Asp Ile Ser Glu Met Lys Asp Met Asn Val Val Glu Gly Arg Lys
65 70 75 80
Phe Gly Ile Leu Gly Asn Lys Val Phe Arg Lys Leu Glu Asn Leu Glu
85 90 95
Lys Pro Val Ile Ala Ala Leu Asn Gly Phe Thr Leu Gly Gly Gly Cys
100 105 110
Glu Ile Ala Met Ser Cys Asp Ile Arg Ile Ala Ser Thr Lys Ala Lys
115 120 125
Phe Gly Gln Pro Glu Val Gln Leu Gly Ile Thr Pro Gly Phe Gly Gly
130 135 140
Thr Gln Arg Leu Ala Arg Leu Ile Gly Pro Gly Ala Ala Lys Glu Leu
145 150 155 160
Ile Tyr Thr Gly Lys Ile Ile Asn Ala Glu Glu Ala Tyr Arg Leu Gly
165 170 175
Leu Val Asn Arg Val Ile Glu Pro Glu Thr Leu Leu Asp Glu Ala Lys
180 185 190
Gln Leu Ala Asn Thr Ile Ala Ala Asn Ala Pro Ile Ala Val Lys Leu
195 200 205
Ala Lys Ser Ala Ile Asn Arg Gly Ile Gln Thr Asp Ile Asp Thr Gly
210 215 220
Val Ser Ile Glu Ser Glu Val Phe Gly Ala Cys Phe Ser Thr Glu Asp
225 230 235 240
Gln Lys Glu Gly Met Asn Thr Phe Leu Asn Asp Lys Lys Tyr Leu Thr
245 250 255
Gly Asn Phe Lys Asn Lys
260
<210> 26
<211> 260
<212> PRT
<213> Megasphaera elsdenii
<400> 26
Met Asp Tyr Gln Asn Ile Ile Phe Ala Val Glu Asp Gly Ile Ala Thr
1 5 10 15
Ile Thr Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Gln Ala Thr
20 25 30
Val Ser Glu Leu Lys Asp Val Val Glu Lys Ile Ala Ala Asp Lys Ala
35 40 45
Ile Lys Val Val Ile Ile Thr Gly Ala Gly Ala Lys Ser Phe Val Ala
50 55 60
Gly Ala Asp Ile Lys Glu Met Ala Ser Lys Asn Ala Ala Glu Gly Arg
65 70 75 80
Glu Trp Gly Gln Phe Gly Gln Asn Val Phe Thr Glu Ile Glu Asn Leu
85 90 95
Pro Gln Pro Val Ile Ala Ala Ile Asn Gly Phe Ala Leu Gly Gly Gly
100 105 110
Cys Glu Leu Ser Cys Ala Cys Asp Ile Arg Tyr Ala Ala Glu Asn Ala
115 120 125
Lys Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly
130 135 140
Gly Thr Gln Arg Leu Thr Arg Val Val Gly Arg Gly His Ala Lys Glu
145 150 155 160
Leu Ile Tyr Thr Gly Gly Met Ile Asp Ala Glu Lys Ala Lys Ala Ile
165 170 175
Gly Leu Val Asn Glu Val Phe Pro Gln Glu Glu Leu Met Pro Ala Ala
180 185 190
Val Lys Leu Ala Lys Lys Ile Ala Lys Asn Ala Pro Ile Ala Val Gln
195 200 205
Leu Ser Lys Ala Ala Ile Asn Arg Gly Ile Asn Cys Asp Val Val Thr
210 215 220
Gly Ile Ala Tyr Glu Ala Glu Val Phe Gly Leu Cys Phe Ser Thr Ala
225 230 235 240
Asp Gln Lys Glu Gly Met Ala Ala Phe Cys Glu Lys Arg Lys Ala Thr
245 250 255
Phe Glu Gly Lys
260
<210> 27
<211> 259
<212> PRT
<213> Metallosphaera sedula
<400> 27
Met Glu Phe Glu Thr Ile Glu Thr Lys Lys Glu Gly Asn Leu Phe Trp
1 5 10 15
Ile Thr Leu Asn Arg Pro Asp Lys Leu Asn Ala Leu Asn Ala Lys Leu
20 25 30
Leu Glu Glu Leu Asp Arg Ala Val Ser Gln Ala Glu Ser Asp Pro Glu
35 40 45
Ile Arg Val Ile Ile Ile Thr Gly Lys Gly Lys Ala Phe Cys Ala Gly
50 55 60
Ala Asp Ile Thr Gln Phe Asn Gln Leu Thr Pro Ala Glu Ala Trp Lys
65 70 75 80
Phe Ser Lys Lys Gly Arg Glu Ile Met Asp Lys Ile Glu Ala Leu Ser
85 90 95
Lys Pro Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu
100 105 110
Glu Leu Ala Leu Ala Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln
115 120 125
Leu Gly Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Tyr Gly Gly
130 135 140
Thr Gln Arg Leu Thr Arg Val Ile Gly Lys Gly Arg Ala Leu Glu Met
145 150 155 160
Met Met Thr Gly Asp Arg Ile Pro Gly Lys Asp Ala Glu Lys Tyr Gly
165 170 175
Leu Val Asn Arg Val Val Pro Leu Ala Asn Leu Glu Gln Glu Thr Arg
180 185 190
Lys Leu Ala Glu Lys Ile Ala Lys Lys Ser Pro Ile Ser Leu Ala Leu
195 200 205
Ile Lys Glu Val Val Asn Arg Gly Leu Asp Ser Pro Leu Leu Ser Gly
210 215 220
Leu Ala Leu Glu Ser Val Gly Trp Gly Val Val Phe Ser Thr Glu Asp
225 230 235 240
Lys Lys Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Glu Pro Thr Phe
245 250 255
Lys Gly Lys
<210> 28
<211> 259
<212> PRT
<213> Clostridicum kluyvery
<400> 28
Met Glu Phe Lys Asn Ile Ile Leu Glu Lys Asp Gly Asn Val Ala Ser
1 5 10 15
Ile Thr Leu Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ala Ala Thr
20 25 30
Leu Lys Glu Ile Asp Ala Ala Ile Asn Asp Ile Ala Glu Asp Asp Asn
35 40 45
Val Tyr Ala Val Ile Ile Thr Gly Ser Gly Lys Ala Phe Val Ala Gly
50 55 60
Ala Asp Ile Ala Glu Met Lys Asp Leu Thr Ala Val Glu Gly Arg Lys
65 70 75 80
Phe Ser Val Leu Gly Asn Lys Ile Phe Arg Lys Leu Glu Asn Leu Glu
85 90 95
Lys Pro Val Ile Ala Ala Ile Asn Gly Phe Ala Leu Gly Gly Gly Cys
100 105 110
Glu Leu Ser Leu Ser Cys Asp Ile Arg Ile Ala Ser Ser Lys Ala Lys
115 120 125
Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly Gly
130 135 140
Thr Gln Arg Leu Ala Arg Ala Ile Gly Val Gly Met Ala Lys Glu Leu
145 150 155 160
Ile Tyr Thr Gly Lys Val Ile Asn Ala Glu Glu Ala Leu Arg Ile Gly
165 170 175
Leu Val Asn Lys Val Val Glu Pro Asp Lys Leu Leu Glu Glu Ala Lys
180 185 190
Ala Leu Val Asp Ala Ile Ile Val Asn Ala Pro Ile Ala Val Arg Met
195 200 205
Cys Lys Ala Ala Ile Asn Gln Gly Leu Gln Cys Asp Ile Asp Thr Gly
210 215 220
Val Ala Tyr Glu Ala Glu Val Phe Gly Glu Cys Phe Ala Thr Glu Asp
225 230 235 240
Arg Val Glu Gly Met Thr Ala Phe Val Glu Lys Arg Asp Lys Ala Phe
245 250 255
Lys Asn Lys
<210> 29
<211> 502
<212> PRT
<213> Sulfolobus tokodaii
<400> 29
Met Ala Ile Arg Thr Gly Glu Gln Tyr Leu Asp Ser Ile Lys Ile Arg
1 5 10 15
Asn Lys Ala Glu Ile Tyr Val Met Gly Lys Glu Val Lys Asp Val Thr
20 25 30
Thr His Pro Phe Leu Lys Pro Ser Val Met Ala Phe Lys Ala Thr Phe
35 40 45
Asp Ala Ala Trp Glu Glu Asp Thr Lys Glu Leu Ala Arg Ala Trp Ser
50 55 60
Pro Phe Ile Asn Glu Glu Val Asn Arg Phe Asn His Ile His Arg Ser
65 70 75 80
Pro Glu Asp Leu Ala Ala Lys Val Lys Leu Leu Arg Lys Leu Ser His
85 90 95
Lys Thr Gly Ala Cys Phe Gln Arg Cys Val Gly Trp Asp Ala Leu Asn
100 105 110
Thr Leu Trp Ile Met Thr Asn Ile Met Ala Gln Lys Gly Lys Lys Glu
115 120 125
Tyr Lys Asp Arg Phe Val Glu Tyr Leu Ser Tyr Val Gln Lys Lys Asp
130 135 140
Leu Ala Leu Ala Gly Ala Met Thr Asp Ala Lys Gly Val Arg Thr Leu
145 150 155 160
Lys Pro His Gln Gln Pro Asn Lys Asn Ala Tyr Val Arg Ile Glu Glu
165 170 175
Val Thr Lys Asp Gly Ile Tyr Val Ser Gly Ala Lys Ala Asn Ile Thr
180 185 190
Gly Val Ala Ala Thr Glu Glu Ile Val Val Leu Pro Thr Arg Ala Met
195 200 205
Gly Pro Glu Asp Lys Asp Tyr Ala Val Ala Phe Ser Ile Pro Thr Asp
210 215 220
Thr Glu Gly Ile Lys Ile Ile Val Gly Arg Gln Leu Asn Asp Ala Arg
225 230 235 240
Arg Leu Glu Gly Gly Asp Ile Asp Ala Leu Pro Tyr Phe Tyr Asn His
245 250 255
Glu Gly Leu Val Ile Phe Asp His Val Phe Val Pro Met Asp Arg Val
260 265 270
Phe Leu Met Gly Glu Tyr Glu Phe Thr Ser Gln Leu Val Glu Val Phe
275 280 285
Ser Ala Tyr His Arg Gln Gly Tyr Gly Gly Cys Lys Ala Gly Leu Gly
290 295 300
Asp Val Ile Ile Gly Ala Ser Met Asn Leu Ala Lys Gln Leu Gly Val
305 310 315 320
Glu Lys Ala Ser His Val Gln Glu Lys Leu Thr Glu Met Ile Phe Leu
325 330 335
Thr Glu Thr Met Tyr Ser Ala Gly Ile Ala Ala Ser Leu Asn Ala Val
340 345 350
Lys Val Cys Asp Asn Cys Trp Trp Val Asn Pro Met His Ala Asn Val
355 360 365
Thr Lys His Leu Val Ala Arg Phe Pro Ala Gln Ile Ser Gln Leu Ser
370 375 380
Ile Asp Ile Ala Gly Gly Ile Ile Gly Thr Ala Pro Ser Glu Trp Asp
385 390 395 400
Leu Lys Asn Pro Lys Leu Arg Glu Tyr Ile Ala Lys Tyr Leu Gln Gly
405 410 415
Val Glu Gly Tyr Thr Ala Glu Asp Arg Leu Arg Met Val Arg Leu Leu
420 425 430
Glu Asn Val Ser Leu Gly Val Ala Phe Gln Ile Glu Ser Val His Gly
435 440 445
Ala Gly Ser Pro Ala Ala Gln Arg Ile Met Phe Ser Arg Leu Tyr Asp
450 455 460
Leu Asn Tyr Ala Glu Glu Val Ala Lys Arg Leu Ala Gly Lys Lys Thr
465 470 475 480
Asp Leu Gln Trp Lys Pro Lys Ala Glu Pro Trp Arg Glu Ser Glu Thr
485 490 495
Glu Lys Leu Val Lys Ser
500
<210> 30
<211> 483
<212> PRT
<213> Geobacter metallireducens
<400> 30
Met Ala Leu Arg Asp Gly Asn Ser Tyr Arg Glu Ser Leu Arg Ala Leu
1 5 10 15
Asn Ile Lys Val Tyr Ala Phe Gly Glu Lys Ile Asp Ser Ile Val Asp
20 25 30
His Pro Leu Phe Gln Pro His Ile Asn Ala Ala Ala Leu Thr Phe Asp
35 40 45
Leu Ala His Asp Pro Thr Thr Glu Ala Leu Val Thr Ala Thr Ser His
50 55 60
Leu Thr Gly Ser Lys Ile Ser Arg Phe Thr His Ile His Gln Ser Thr
65 70 75 80
Asp Asp Leu Ile Lys Lys Val Lys Met Leu Arg Leu Ile Ala Gly Lys
85 90 95
Thr Gly Ser Cys Tyr Gln Arg Cys Val Gly Trp Asp Ala Leu Asn Ala
100 105 110
Asn Tyr Thr Val Thr Tyr Glu Met Asp Gln Glu Leu Gly Thr Asp Tyr
115 120 125
His Gln Arg Phe Arg Arg Tyr Leu Glu Tyr Ile Gln Asp Asn Asp Leu
130 135 140
Met Val Ala Gly Ala Met Thr Asp Pro Lys Gly Asp Arg Gly Leu Pro
145 150 155 160
Pro Ala Lys Gln Lys Asp Pro Asp Met Phe Val His Val Val Ala Lys
165 170 175
Asn Asp Lys Gly Ile Val Ile Arg Gly Ala Lys Val His Gln Thr Gly
180 185 190
Ile Val Asn Ser His Glu Met Leu Ile Met Pro Thr Met Ala Met Gly
195 200 205
Glu Glu Asp Gly Asp Tyr Ala Val Ala Cys Ala Leu Pro Thr Asp Ser
210 215 220
Pro Gly Val Ile His Ile Phe Gly Arg Gln Thr Asn Asp Thr Arg Arg
225 230 235 240
Leu Glu Lys Gly Asp Leu Asp Gln Gly Asn Ala Glu Tyr Gly Thr Val
245 250 255
Gly Gly Glu Ala Leu Thr Ile Leu Glu Asp Val Phe Val Pro Trp Glu
260 265 270
Arg Val Phe Met Cys Gly Glu Tyr Lys Tyr Ala Gly Leu Leu Val Glu
275 280 285
Arg Phe Ala Ser Tyr His Arg Gln Asn Tyr Gly Gly Cys Lys Ala Gly
290 295 300
Val Ser Asp Val Ile Ile Gly Ala Thr Thr Ala Met Ala Glu Tyr Asn
305 310 315 320
Gly Ala Ala Lys Ala Ser His Val Arg Asp Lys Ile Val Glu Met Val
325 330 335
His Leu Thr Glu Thr Leu Tyr Cys Gly Ser Ile Ala Cys Ser Cys Glu
340 345 350
Gly Ala Pro Thr Pro Ser Gly Ala Tyr Phe Val Asn Pro Leu Leu Ala
355 360 365
Asn Thr Val Lys Gln Asn Val Thr Arg Phe Ile Tyr Glu Ile Ala Arg
370 375 380
Leu Ser His Asp Ile Ser Gly Gly Cys Met Ala Thr Met Pro Ser Glu
385 390 395 400
Lys Asp Leu His His Asp Glu Ile Gly Lys Tyr Val Glu Lys Tyr Phe
405 410 415
Arg Gly Val Asp Glu Ala Pro Thr Glu Glu Arg Met Arg Met Ala Arg
420 425 430
Leu Val Glu Asn Met Thr Gly Gly Thr Ala Leu Val Glu Ser Met His
435 440 445
Gly Ala Gly Ser Pro Gln Ala Gln Arg Val Met Ile Leu Arg Gln Ala
450 455 460
Asn Leu Gly His Lys Val Lys Leu Ala Lys Lys Leu Ala Gly Ile Lys
465 470 475 480
Glu Glu Lys
<210> 31
<211> 463
<212> PRT
<213> Sulfolobus solfataricus
<400> 31
Met Arg Ser Lys Glu Asp Phe Leu Lys Ser Leu Lys Asp Gly Arg Asn
1 5 10 15
Leu Tyr Tyr Arg Gly Lys Leu Val Glu Asp Ile Thr Thr His Gln Ile
20 25 30
Leu Lys Thr Ala Ala Leu His Ala Ala Lys Leu Tyr Glu Tyr Ala Asp
35 40 45
Arg Val Tyr Glu Asp Asn Lys Met Gly Lys Met Ser Lys Phe Phe Lys
50 55 60
Val Pro Trp Thr Ser Gln Asp Leu Leu Asp Arg His Lys Leu Ile Tyr
65 70 75 80
Asp Leu Thr Met Tyr Cys Asn Gly Val Phe Asn Ile Ser Gln Ala Ile
85 90 95
Gly Ser Asp Ala Ile Phe Ala Leu Met Ile Thr Ala Lys Gln Val Asp
100 105 110
Arg Lys Tyr Gly Thr Asp Tyr Ser Lys Arg Val Glu Lys Tyr Phe Glu
115 120 125
Arg Val Ala Lys Glu Asp Leu Thr Leu Ala Thr Ala Gln Thr Asp Val
130 135 140
Lys Gly Asp Arg Ser Lys Arg Pro Ser Glu Gln Val Asp Pro Asp Met
145 150 155 160
Tyr Val Arg Val Val Asp Val Lys Ser Asp Gly Ile Val Val Arg Gly
165 170 175
Ala Lys Ala His Thr Thr Gln Ser Ala Val Ser Asp Glu Ile Ile Val
180 185 190
Ile Pro Thr Arg Val Met Arg Asp Ser Asp Lys Asp Tyr Ala Val Ala
195 200 205
Phe Ala Val Pro Ala Asn Thr Lys Gly Leu Lys Met Tyr Ile Arg Pro
210 215 220
Ile Asp Glu Ile Glu Gly Asn Ser Ser Ser Val Leu Ser Arg Lys Asp
225 230 235 240
Tyr Glu Leu Glu Thr Leu Thr Val Phe Asn Asp Val Phe Val Pro Trp
245 250 255
Asp Arg Val Phe Leu Phe Lys Glu Tyr Asp Tyr Ala Gly Thr Leu Ala
260 265 270
Met Leu Phe Ala Thr Phe His Arg Phe Thr Ala Leu Ser Tyr Arg Ser
275 280 285
Ala Thr Met Asn Leu Tyr Leu Gly Ala Ser Lys Val Ala Ser Gln Val
290 295 300
Asn Gly Ile Glu Asn Glu Lys His Val Arg Asp Asp Ile Val Asp Ile
305 310 315 320
Ile Leu Tyr Lys Glu Ile Met Arg Ser Ser Ala Ile Ala Ala Ala Val
325 330 335
Tyr Pro Val Asn Met Glu Gly Ile Ala Val Pro Asn Pro Leu Phe Thr
340 345 350
Asn Val Gly Lys Leu Tyr Ser Asn Met His Phe His Asp Val Val Arg
355 360 365
Asp Leu Ile Asp Ile Ala Gly Gly Ile Ile Ala Thr Met Pro Ser Gln
370 375 380
Glu Asp Leu Glu Ser Asp Glu Gly Lys Asn Ile Val Lys Tyr Leu Arg
385 390 395 400
Gly Ser Val Asp Gly Glu Glu Arg Ala Lys Val Leu Lys Leu Ala Lys
405 410 415
Glu Leu Gly Ala Ser Thr Phe Thr Gly Tyr Leu Leu Thr Gly Met Ile
420 425 430
His Ala Glu Gly Ser Met Glu Ala Ser Lys Ile Glu Leu Phe Arg Ser
435 440 445
Tyr Asn Phe Lys Glu Ala Glu Asn Leu Val Lys Arg Val Leu Ser
450 455 460
<210> 32
<211> 479
<212> PRT
<213> Syntrophobacter fumaroxidans
<400> 32
Met Gly Leu Lys Thr Lys Ala Glu Tyr Ile Glu Ser Leu Arg Gly Met
1 5 10 15
Lys Pro Thr Val Tyr Met Phe Gly Glu Lys Ile Glu Ser Val Val Asp
20 25 30
Asn Pro Arg Leu Arg Ala Gly Ile Glu Ala Thr Gly Ala Thr Tyr Glu
35 40 45
Leu Ala Glu Thr Glu Glu Tyr Arg Pro Leu Ile Val Thr Glu Ser Pro
50 55 60
Leu Ile His Glu Pro Val Asn Arg Tyr Thr Leu Pro Pro Ser Ser Ile
65 70 75 80
Ala Asp Leu Val Ala Arg Val Lys Ile Asn Arg Leu Met Gly Thr Arg
85 90 95
Val Gly Thr Cys Phe Gln Arg Cys Thr Gly Leu Asp Cys Leu Ser Ala
100 105 110
Leu Ser Ile Val Thr Tyr Asp Ile Asp Ala Lys His Ser Thr Pro Tyr
115 120 125
Phe Lys Arg Phe Ile Glu Phe Leu Lys His Val Gln Lys Asn Asp Leu
130 135 140
Thr Cys Asn Ala Gly Val Thr Asp Val Lys Gly Asp Arg Ser Leu Ala
145 150 155 160
Pro His Glu Gln Glu Asp Lys Asp Met Tyr Val Arg Val Val Glu Arg
165 170 175
Asn Ala Asp Gly Ile Val Val Arg Gly Ala Lys Ala His Gln Thr Gly
180 185 190
Ser Leu Ser Ser His Glu Ile Ile Val Leu Pro Thr Arg Ala Leu Arg
195 200 205
Lys Gly Asp Glu Asp Tyr Ala Leu Ala Phe Ala Ile Pro Asn Asp Thr
210 215 220
Pro Gly Leu Ile His Val Val Gly Arg Ser Ser Leu Asp Thr Arg Gln
225 230 235 240
Leu Asp Gly Cys Asp Leu Gly Asn Leu His Tyr Ser Lys Tyr Cys Pro
245 250 255
Thr Val Ile Phe Lys Asp Val Phe Val Pro Trp Glu Arg Val Phe Met
260 265 270
Cys Gly Glu Val Glu Phe Ala Val Glu Met Val Asn Arg Phe Ser Ala
275 280 285
Tyr His Arg Gln Ser His Gly Gly Cys Lys Ser Gly Lys Ile Asp Cys
290 295 300
Met Val Gly Ala Ala Leu Thr Met Met Asp Tyr Asn Gly Thr Glu Lys
305 310 315 320
Ala Gly His Leu Lys Gln Lys Ala Ile Glu Met Val His Arg Ala Glu
325 330 335
Thr Leu Tyr Gly Cys Ser Leu Ala Ala Ser Tyr Glu Gly Lys Lys Glu
340 345 350
Pro Ser Gly Thr Tyr Phe Ile Asp Thr Val Leu Ala Asn Ala Ser Lys
355 360 365
Ile His Glu Gly Lys Glu Met Ser Glu Ala Gly Arg Leu Leu Val Asp
370 375 380
Ile Ala Gly Gly Phe Val Ala Asp Leu Pro Ser Asp Arg Asp Leu Ala
385 390 395 400
Ile Pro Glu Val Gly Glu Leu Leu Lys Lys Tyr Leu Lys Gly Val Ala
405 410 415
Ser Val Pro Val Glu Asp Arg Val Lys Met Tyr Arg Leu Ile Glu Lys
420 425 430
Leu Val Met Glu Ser Ala Asp Thr Ile Ser Asp Ile His Gly Gly Gly
435 440 445
Ser Pro Glu Ala His Arg Ile Thr Ile Leu Arg Glu Ser Asn Leu Lys
450 455 460
Ala Lys Lys Asp Ala Ala Lys Arg Leu Ala Gly Ile Glu Ser Lys
465 470 475
<210> 33
<211> 486
<212> PRT
<213> Porphyromonas gingivalis
<400> 33
Met Met Thr Ser Glu Gln Tyr Val Glu Ser Leu Arg Lys Leu Asn Leu
1 5 10 15
Lys Val Tyr Phe Met Gly Glu Arg Ile Glu Asn Pro Val Asp His Pro
20 25 30
Met Ile Arg Pro Ser Met Asn Ser Val Ala Met Thr Tyr Lys Leu Ala
35 40 45
Glu Met Asp Glu Tyr Lys His Leu Met Thr Ala Thr Ser Asn Leu Thr
50 55 60
Gly Lys Gln Val Asn Arg Phe Cys His Leu His Gln Ser Thr Glu Asp
65 70 75 80
Leu Lys Asp Lys Val Lys Met Gln Arg Leu Met Gly Gln Lys Thr Ala
85 90 95
Ser Cys Phe Gln Arg Cys Val Gly Met Asp Ala Phe Asn Ala Ile Tyr
100 105 110
Ser Thr Thr Tyr Glu Met Asp Gln Ala Leu Gly Thr Thr Tyr His Lys
115 120 125
Arg Phe Ile Glu Tyr Met Lys Tyr Val Gln Asp Asn Asp Leu Val Val
130 135 140
Asp Gly Ala Met Thr Asp Pro Lys Gly Asp Arg Gly Leu Ser Pro Ser
145 150 155 160
Glu Gln Ala Asp Pro Asp Leu Tyr Leu His Ile Val Glu Val Arg Glu
165 170 175
Asp Gly Ile Val Val Ser Gly Ala Lys Ala His Gln Thr Gly Ala Val
180 185 190
Asn Ser His Glu His Leu Ile Met Pro Thr Ile Ala Met Arg Glu Ala
195 200 205
Asp Ala Asp Tyr Ala Val Ser Phe Ala Val Pro Ser Asp Ala Glu Gly
210 215 220
Val Ile Met Ile Tyr Gly Arg Gln Ser Cys Asp Thr Arg Lys Met Glu
225 230 235 240
Glu Gly Ala Asp Ile Asp Leu Gly Asn Ser Glu Phe Gly Gly His Glu
245 250 255
Ala Leu Val Val Phe Asp Arg Val Phe Val Pro Asn Asp Arg Val Phe
260 265 270
Met Cys Lys Glu Tyr Gln Phe Ala Gly Met Met Val Glu Arg Phe Ala
275 280 285
Gly Tyr His Arg Gln Ser Tyr Gly Gly Cys Lys Val Gly Val Gly Asp
290 295 300
Val Leu Ile Gly Ala Ala Ala Leu Ala Ala Asp Tyr Asn Gly Val Pro
305 310 315 320
Lys Ala Ser His Ile Lys Asp Lys Leu Ile Glu Met Ile His Leu Asn
325 330 335
Glu Thr Leu Tyr Ala Cys Gly Ile Ala Cys Ser Ser Glu Gly Thr Gln
340 345 350
Met Lys Ala Gly Asn Tyr Met Ile Asp Leu Leu Leu Ala Asn Val Cys
355 360 365
Lys Gln Asn Ile Thr Arg Leu Pro Tyr Glu Ile Ala Arg Leu Ala Glu
370 375 380
Asp Ile Ala Gly Gly Leu Met Val Thr Met Pro Ser Gln Gln Asp Phe
385 390 395 400
Arg His Pro Glu Ile Gly Pro Ile Val Lys Lys Tyr Leu Ala Gly Ala
405 410 415
Thr Gly Lys Ser Thr Glu Asn Arg Met Arg Val Leu Arg Leu Ile Glu
420 425 430
Asn Ile Thr Leu Gly Thr Ala Ala Val Gly Tyr Arg Thr Glu Ser Met
435 440 445
His Gly Ala Gly Ser Pro Gln Ala Gln Arg Ile Met Ile Ala Arg Gln
450 455 460
Gly Asp Leu Glu Gly Lys Lys Lys Leu Ala Arg Ala Ile Ala His Ile
465 470 475 480
Asp Glu Ser Leu Asp Lys
485
<210> 34
<211> 529
<212> PRT
<213> Polynucleobacter necessarius subsp. Asymbioticus
<400> 34
Met Ser Gln Ser Thr Ser Gln Phe Met Asn Ser Lys Asp Tyr Gln Glu
1 5 10 15
Ser Leu Arg Ser Leu Lys Pro Thr Val Tyr Val Asp Gly Arg Leu Ile
20 25 30
Glu Ser Val Ala Asp Glu Pro Ser Leu Arg Pro Gly Val Gln Ala Leu
35 40 45
Gly Val Thr Tyr Asp Met Val His Asp Pro Ala Leu Ala Pro Leu Met
50 55 60
Leu Ala Asp Ser Asn Gly Thr Pro Val Pro Arg Met Leu His Ile Asn
65 70 75 80
Gln Ser Ser Gly Asp Leu Leu Asn Lys Leu Glu Ala Val Arg Val Leu
85 90 95
Cys Gln Glu Thr Gly Cys Ala Gln Arg Tyr Leu Ala His Asp Ala Leu
100 105 110
Asn Ala Ile Ala Gln Val Ser Ala Arg Ile Asp Asp Ala Lys Gly Ser
115 120 125
Asn Glu His Ser Ala Lys Phe Ser Glu Tyr Leu Ser His Val Gln Thr
130 135 140
Lys Asp Leu Ala Leu Gly Ile Ala Met Thr Asp Ala Lys Gly Asp Arg
145 150 155 160
Ser Arg Arg Pro His Glu Gln Glu Asn Pro Asp Thr Tyr Val His Ile
165 170 175
Val Ser Gln Asp Ala Lys Gly Val Val Ile Ser Gly Thr Lys Ala Ile
180 185 190
Val Thr Gly Ala Pro Tyr Met His Glu Phe Leu Val Met Pro Gly Arg
195 200 205
Asn Met Thr Lys Glu Asp Ala Ala Phe Ala Ile Cys Cys Ala Val Pro
210 215 220
Val Asp Ala Lys Gly Ile Thr Ile Val Ala Arg Pro Ala Gly Arg Pro
225 230 235 240
Gly Asp Lys Val Glu His Gly Lys Pro Ile Phe Ser Ser Lys Tyr Gly
245 250 255
Gln Ser Thr Gly Val Val Ile Phe Asp Lys Val Phe Val Pro Trp Asp
260 265 270
Arg Val Phe Tyr Ala Gly Glu Trp Glu His Ser Ser Val Leu Thr Tyr
275 280 285
Asn Tyr Ala Thr His His Arg His Ser Cys Ile Ala Ala Arg Ala Gly
290 295 300
Phe Gly Asp Leu Leu Ile Gly Ala Gly Ala Leu Met Cys Glu Ala Asn
305 310 315 320
Gly Leu Asp Pro Ala Thr Lys Ser Asn Leu Arg Asp Pro Met Val Glu
325 330 335
Leu Ile Lys Ile Thr Glu Gly Phe Tyr Ala Cys Gly Val Ala Ala Ser
340 345 350
Val Tyr Gly Thr Gln Asp Pro Tyr Ser Lys Ser Phe Met Pro Glu Pro
355 360 365
Val Phe Ser Asn Ile Gly Lys Leu Leu Leu Ala Thr Gln Ile Tyr Asp
370 375 380
Met His Arg Leu Ala His Glu Val Ser Gly Gly Leu Ile Val Ala Leu
385 390 395 400
Pro Gly Pro Asp Glu Asp His Asn Pro Ala Thr Ala Ala Thr Leu Ala
405 410 415
Glu Val Leu Arg Ala Asn Pro Ala Val Pro Tyr Asp Lys Arg Ile Glu
420 425 430
Val Ala Arg Phe Ile Glu Asp Leu Thr Ala Ser Tyr Gln Gly Gly Trp
435 440 445
Tyr Ser Val Ile Ser Leu His Gly Gly Gly Ser Pro Ala Ala Met Lys
450 455 460
Gln Glu Ile Tyr Arg Gln Tyr Pro Ile Gly Asn Lys Val Glu Leu Val
465 470 475 480
Glu Arg Leu Leu Asp Arg Gly Val Leu Thr Ser Ser Glu Glu Arg Ala
485 490 495
Ile Thr Lys Asn Lys Gln Pro Gly Arg Cys Cys Asp Gln Gly Cys Ser
500 505 510
Ala Pro Gly Gln Ala Val Met Val Pro Leu Pro Glu Pro Gly Arg Arg
515 520 525
Thr
<210> 35
<211> 257
<212> PRT
<213> Sulfolobus tokodaii
<400> 35
Met Glu Thr Ile Val Ile Lys Lys Glu Thr Pro Ile Gly Trp Ile Tyr
1 5 10 15
Leu Asn Arg Pro Asp Arg Leu Asn Ala Ile Asn Gln Gln Met Ile Lys
20 25 30
Glu Leu Arg Gln Gly Ile Asp Glu Met Val Tyr Asp Ser Asp Ile Lys
35 40 45
Val Ile Ile Ile Thr Gly Asn Gly Lys Ala Phe Ser Ala Gly Ala Asp
50 55 60
Ile Ser Arg Phe Lys Glu Leu Asn Gly Tyr Thr Ala Trp Gln Phe Ala
65 70 75 80
Lys Ser Gly Arg Glu Leu Met Asp Tyr Ile Glu Asn Ile Ser Lys Pro
85 90 95
Thr Ile Ala Met Val Asn Gly Tyr Ala Leu Gly Gly Gly Leu Glu Leu
100 105 110
Ala Met Ala Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln Leu Gly
115 120 125
Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Phe Gly Gly Thr Gln
130 135 140
Arg Leu Val Arg Leu Ile Gly Lys Gly Lys Ala Leu Glu Leu Met Leu
145 150 155 160
Thr Gly Asp Arg Ile Ser Ala Lys Glu Ala Glu Lys Ile Gly Leu Val
165 170 175
Asn Lys Val Val Pro Leu Ser Asn Leu Glu Gln Glu Thr Arg Asn Phe
180 185 190
Ala Leu Lys Leu Ala Glu Lys Pro Pro Ile Ser Ile Ala Leu Ile Lys
195 200 205
Leu Leu Val Asn Gln Gly Ile Asp Leu Pro Ile Leu Ala Gly Leu Asn
210 215 220
Met Glu Ser Leu Gly Trp Gly Val Val Phe Ser Thr Glu Asp Glu Lys
225 230 235 240
Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Lys Ala Gln Phe Lys Gly
245 250 255
Lys
<210> 36
<211> 258
<212> PRT
<213> Gordonia terrae C-6
<400> 36
Met Thr Glu His Gln Thr Ile Val Val Glu Thr Ser Gly Arg Val Gly
1 5 10 15
Ile Ile Thr Leu Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Thr Glu
20 25 30
Leu Met Asn Glu Val Val Gly Ala Val Lys Glu Phe Asp Val Asp Gln
35 40 45
Gly Ile Gly Ala Ile Val Ile Thr Gly Ser Glu Lys Ala Phe Ala Ala
50 55 60
Gly Ala Asp Ile Lys Glu Met Ser Ser Lys Ser Tyr Ala Asp Val Val
65 70 75 80
Asn Glu Gln Phe Phe Gly Ala Trp Asp Glu Leu Ser Arg Ala Arg Thr
85 90 95
Pro Ile Ile Ala Ala Val Thr Gly Tyr Ala Leu Gly Gly Gly Cys Glu
100 105 110
Leu Ala Met Leu Cys Asp Thr Ile Ile Ala Gly Asp Asn Ala Val Phe
115 120 125
Gly Gln Pro Glu Ile Asn Leu Gly Val Ile Pro Gly Ile Gly Gly Ser
130 135 140
Gln Arg Leu Thr Arg Ala Val Gly Lys Ala Lys Ala Met Asp Met Val
145 150 155 160
Leu Thr Gly Arg Gln Met Lys Val Asp Glu Ala Glu Arg Leu Gly Leu
165 170 175
Val Ser Arg Val Val Pro Lys Glu Asp Cys Arg Ala Ala Ala Ile Glu
180 185 190
Val Ala Glu Ile Ile Ala Ser Lys Ser Leu Ile Ala Ala Ala Ala Ala
195 200 205
Lys Asp Ala Val Asn Arg Ala Phe Glu Ser Ser Leu Val Glu Gly Val
210 215 220
Arg Ala Glu Arg Ala Leu Phe Tyr Ser Thr Phe Ala Thr Asp Asp Gln
225 230 235 240
Thr Glu Gly Met Ala Ala Phe Val Glu Lys Arg Asp Pro Asn Phe Thr
245 250 255
His Arg
<210> 37
<211> 258
<212> PRT
<213> Halalkalicoccus jeotgali
<400> 37
Met Ala Asp Arg Val Leu Ile Glu Arg Glu Asn Asp Ile Ala Thr Ile
1 5 10 15
Ile Val Asn Arg Pro Glu Lys Arg Asn Ala Met Asp Ile Pro Thr Arg
20 25 30
Lys Ala Leu Tyr Ala Ala Phe Glu Glu Val Ser Glu Asp Asp Asp Val
35 40 45
Arg Ala Ile Val Leu Arg Gly Ala Gly Asp Gly Ser Phe Ile Ala Gly
50 55 60
Gly Asp Ile Asp Ser Phe Ala Asp Phe Asp His Met Asp Gly Met Glu
65 70 75 80
Tyr Ser Glu Lys Tyr Ala Gln Gly Leu Tyr Asn Tyr Val Ala Asp Arg
85 90 95
His Lys Pro Thr Ile Ala Ala Val Asp Gly Tyr Ala Leu Gly Gly Gly
100 105 110
Thr Glu Ile Ala Leu Ala Cys Asp Ile Arg Leu Ala Thr Asp Asp Ala
115 120 125
Lys Phe Gly Leu Pro Glu Val Gly Ile Gly Val Ile Pro Ala Gly Gly
130 135 140
Gly Thr Gln Arg Leu Val Gln Val Val Gly Ala Gly Leu Ala Ser Glu
145 150 155 160
Leu Ile Leu Thr Gly Arg Ile Ile Ser Ala Asp Glu Ala Lys Arg Ile
165 170 175
Gly Leu Ala Asn His Val Tyr Ala Ala Glu Glu Phe Asp Asn Glu Val
180 185 190
Arg Ala Met Ala Glu Asp Leu Ala Ser Lys Ala Pro Val Ala Gln Arg
195 200 205
Leu Ala Lys Glu Ser Ile Arg Arg Ser Leu Asp Ile Asp Ala Gly Leu
210 215 220
Glu Tyr Glu Arg Leu Ala Gly Ala Phe Leu Phe Gly Thr Asp Asp Gln
225 230 235 240
Lys Glu Gly Ala Asn Ala Phe Leu Glu Asp Arg Glu Pro Lys Tyr Arg
245 250 255
Asn Arg
<210> 38
<211> 257
<212> PRT
<213> Carboxydothermus hydrogenoformans
<400> 38
Met Glu Phe Glu Lys Ile Lys Phe Glu Val Thr Asp Gly Tyr Ala Val
1 5 10 15
Ile Tyr Leu Asn Asn Pro Pro Val Asn Ala Leu Gly Gln Lys Val Leu
20 25 30
Lys Asp Leu Gln Lys Ala Leu Gln Glu Ile Glu Lys Asn Pro Glu Ile
35 40 45
Arg Ala Val Ile Ile Ser Gly Glu Gly Ser Lys Val Phe Cys Ala Gly
50 55 60
Ala Asp Ile Thr Glu Phe Ala Asp Arg Ala Lys Gly Ile Leu Pro Glu
65 70 75 80
Val Glu Gly Ser Val Leu Phe Arg Gln Ile Glu Leu Phe Pro Lys Pro
85 90 95
Val Ile Ala Ala Leu Asn Gly Ser Ser Tyr Gly Gly Gly Thr Glu Leu
100 105 110
Ala Ile Ser Cys His Leu Arg Ile Leu Ala Asp Asp Ala Ser Met Ala
115 120 125
Leu Pro Glu Val Lys Leu Gly Ile Ile Pro Gly Trp Gly Gly Thr Gln
130 135 140
Arg Leu Pro Arg Leu Ile Gly Lys Thr Arg Ala Leu Glu Ala Met Leu
145 150 155 160
Thr Gly Glu Pro Ile Thr Ala Glu Glu Ala Leu Ser Tyr Gly Leu Val
165 170 175
Asn Lys Val Val Pro Lys Asp Gln Val Leu Thr Glu Ala Arg Ala Leu
180 185 190
Ala Ala Lys Leu Ala Lys Gly Ala Pro Ile Ala Met Arg Glu Ile Leu
195 200 205
Lys Ala Val Thr Leu Gly Leu Asp Thr Ser Ile Glu Glu Gly Leu Lys
210 215 220
Ile Glu Lys Glu Gly Ser Lys Val Ala Phe Ser Ser Glu Asp Ala Val
225 230 235 240
Glu Gly Arg Thr Ala Phe Phe Glu Lys Arg Pro Pro Asn Phe Lys Gly
245 250 255
Arg
<210> 39
<211> 257
<212> PRT
<213> Thermomicrobium roseum
<400> 39
Met Ser Val Arg Val Glu Arg Glu Gly Ala Ile Thr Leu Val Thr Val
1 5 10 15
Glu Arg Pro Glu Arg Leu Asn Ala Leu Asp Thr Ala Thr Leu Arg Ala
20 25 30
Leu Leu Ala Ala Val Gln Glu Leu Ala Thr Glu Glu Ala Ile Ala Val
35 40 45
Val Val Leu Thr Gly Ala Gly Asp Arg Ala Phe Ile Ala Gly Ala Asp
50 55 60
Ile Ser Glu Met Val Glu Lys Ser Pro Ala Glu Ala Leu Ala Phe Ala
65 70 75 80
Glu Leu Gly His Ala Val Cys Arg Ala Ile Glu Glu Ala Pro Gln Pro
85 90 95
Tyr Ile Ala Ala Val Asn Gly Tyr Ala Leu Gly Gly Gly Cys Glu Ile
100 105 110
Ala Leu Ala Cys Asp Ile Arg Leu Ala Ser Glu Arg Ala Val Phe Ala
115 120 125
Gln Pro Glu Val Thr Leu Gly Ile Pro Pro Gly Trp Gly Gly Ser Gln
130 135 140
Arg Leu Pro Arg Val Val Pro Pro Gly Ile Ala Arg Glu Leu Leu Tyr
145 150 155 160
Thr Gly Arg Arg Val Asp Ala Gln Glu Ala Leu Arg Ile Gly Leu Val
165 170 175
Asn Ala Val Tyr Pro Ala Asp Gln Leu Leu Glu Arg Ala Arg Glu Leu
180 185 190
Ala Asn Arg Ile Ala Ala Asn Gly Pro Leu Ala Val Arg Leu Thr Lys
195 200 205
Ala Ala Val Arg Phe Gly Leu Glu Gln Gly Leu Glu Ala Gly Leu Thr
210 215 220
Tyr Glu Arg Gln Val Phe Ala Tyr Ala Phe Thr Thr Glu Asp Gln Arg
225 230 235 240
Glu Gly Met Arg Ala Phe Leu Glu Lys Arg Arg Pro Ala Phe Arg Gly
245 250 255
Arg
<210> 40
<211> 274
<212> PRT
<213> Methylobacterium extorquens
<400> 40
Met Asn Ala Asp Ala Glu Thr Ala Ser Thr Asp Glu Leu Leu Phe Ala
1 5 10 15
Val Asp Ala Ala Gly Ile Ala Arg Ile Thr Leu Asn Arg Pro Lys Ala
20 25 30
Arg Asn Ala Leu Thr Phe Ala Met Tyr Arg Gly Leu Val Glu Leu Cys
35 40 45
Glu Arg Ile Glu Ala Asp His Ala Ile Lys Ala Val Ile Ile Thr Gly
50 55 60
Ala Gly Asp Lys Ala Phe Ala Ala Gly Thr Asp Ile Ala Gln Phe Arg
65 70 75 80
Ser Phe Ser Lys Pro Glu Asp Ala Ile Gly Tyr Glu Arg Phe Met Asp
85 90 95
Arg Val Leu Gly Gly Leu Glu Arg Leu Arg Val Pro Thr Ile Ala Ala
100 105 110
Val Ala Gly Ala Cys Thr Gly Gly Gly Ala Ala Ile Ala Ala Ala Cys
115 120 125
Asp Met Arg Ile Ala Ser Arg Asp Ala Arg Phe Gly Ile Pro Ile Ala
130 135 140
Arg Thr Leu Gly Asn Cys Leu Ser Gln Asn Thr Leu Arg Arg Leu Ala
145 150 155 160
Asn Leu Ile Gly Ala Pro Arg Val Lys Asp Ile Leu Phe Thr Ala Arg
165 170 175
Leu Val Glu Ala Gln Glu Ala Leu Ala Ile Gly Leu Val Asn Glu Val
180 185 190
Val Glu Asp Ala Ala Ala Val Ala Ala Arg Ala Asp Ala Leu Ala Thr
195 200 205
Leu Leu Ala Ser His Ala Pro Leu Thr Leu Gln Ala Thr Lys Glu Gly
210 215 220
Leu Arg Arg Ile Gly Glu Glu Gly Ala Ala Glu Ala Ala Glu Gly Glu
225 230 235 240
Arg Pro Gly Asp Asp Leu Ile Val Met Thr Tyr Met Ser Ala Asp Phe
245 250 255
Arg Glu Gly Met Glu Ala Phe Leu Gly Lys Arg Pro Pro Asn Phe Lys
260 265 270
Gly Arg
<210> 41
<211> 407
<212> PRT
<213> Clostridium sporogenes
<400> 41
Met Ser Asp Arg Asn Lys Glu Val Lys Glu Lys Lys Ala Lys His Tyr
1 5 10 15
Leu Arg Glu Ile Thr Ala Lys His Tyr Lys Glu Ala Leu Glu Ala Lys
20 25 30
Glu Arg Gly Glu Lys Val Gly Trp Cys Ala Ser Asn Phe Pro Gln Glu
35 40 45
Ile Ala Thr Thr Leu Gly Val Lys Val Val Tyr Pro Glu Asn His Ala
50 55 60
Ala Ala Val Ala Ala Arg Gly Asn Gly Gln Asn Met Cys Glu His Ala
65 70 75 80
Glu Ala Met Gly Phe Ser Asn Asp Val Cys Gly Tyr Ala Arg Val Asn
85 90 95
Leu Ala Val Met Asp Ile Gly His Ser Glu Asp Gln Pro Ile Pro Met
100 105 110
Pro Asp Phe Val Leu Cys Cys Asn Asn Ile Cys Asn Gln Met Ile Lys
115 120 125
Trp Tyr Glu His Ile Ala Lys Thr Leu Asp Ile Pro Met Ile Leu Ile
130 135 140
Asp Ile Pro Tyr Asn Thr Glu Asn Thr Val Ser Gln Asp Arg Ile Lys
145 150 155 160
Tyr Ile Arg Ala Gln Phe Asp Asp Ala Ile Lys Gln Leu Glu Glu Ile
165 170 175
Thr Gly Lys Lys Trp Asp Glu Asn Lys Phe Glu Glu Val Met Lys Ile
180 185 190
Ser Gln Glu Ser Ala Lys Gln Trp Leu Arg Ala Ala Ser Tyr Ala Lys
195 200 205
Tyr Lys Pro Ser Pro Phe Ser Gly Phe Asp Leu Phe Asn His Met Ala
210 215 220
Val Ala Val Cys Ala Arg Gly Thr Gln Glu Ala Ala Asp Ala Phe Lys
225 230 235 240
Met Leu Ala Asp Glu Tyr Glu Glu Asn Val Lys Thr Gly Lys Ser Thr
245 250 255
Tyr Arg Gly Glu Glu Lys Gln Arg Ile Leu Phe Glu Gly Ile Ala Cys
260 265 270
Trp Pro Tyr Leu Arg His Lys Leu Thr Lys Leu Ser Glu Tyr Gly Met
275 280 285
Asn Val Thr Ala Thr Val Tyr Ala Glu Ala Phe Gly Val Ile Tyr Glu
290 295 300
Asn Met Asp Glu Leu Met Ala Ala Tyr Asn Lys Val Pro Asn Ser Ile
305 310 315 320
Ser Phe Glu Asn Ala Leu Lys Met Arg Leu Asn Ala Val Thr Ser Thr
325 330 335
Asn Thr Glu Gly Ala Val Ile His Ile Asn Arg Ser Cys Lys Leu Trp
340 345 350
Ser Gly Phe Leu Tyr Glu Leu Ala Arg Arg Leu Glu Lys Glu Thr Gly
355 360 365
Ile Pro Val Val Ser Phe Asp Gly Asp Gln Ala Asp Pro Arg Asn Phe
370 375 380
Ser Glu Ala Gln Tyr Asp Thr Arg Ile Gln Gly Leu Asn Glu Val Met
385 390 395 400
Val Ala Lys Lys Glu Ala Glu
405
<210> 42
<211> 374
<212> PRT
<213> Clostridium sporogenes
<400> 42
Met Ser Asn Ser Asp Lys Phe Phe Asn Asp Phe Lys Asp Ile Val Glu
1 5 10 15
Asn Pro Lys Lys Tyr Ile Met Lys His Met Glu Gln Thr Gly Gln Lys
20 25 30
Ala Ile Gly Cys Met Pro Leu Tyr Thr Pro Glu Glu Leu Val Leu Ala
35 40 45
Ala Gly Met Phe Pro Val Gly Val Trp Gly Ser Asn Thr Glu Leu Ser
50 55 60
Lys Ala Lys Thr Tyr Phe Pro Ala Phe Ile Cys Ser Ile Leu Gln Thr
65 70 75 80
Thr Leu Glu Asn Ala Leu Asn Gly Glu Tyr Asp Met Leu Ser Gly Met
85 90 95
Met Ile Thr Asn Tyr Cys Asp Ser Leu Lys Cys Met Gly Gln Asn Phe
100 105 110
Lys Leu Thr Val Glu Asn Ile Glu Phe Ile Pro Val Thr Val Pro Gln
115 120 125
Asn Arg Lys Met Glu Ala Gly Lys Glu Phe Leu Lys Ser Gln Tyr Lys
130 135 140
Met Asn Ile Glu Gln Leu Glu Lys Ile Ser Gly Asn Lys Ile Thr Asp
145 150 155 160
Glu Ser Leu Glu Lys Ala Ile Glu Ile Tyr Asp Glu His Arg Lys Val
165 170 175
Met Asn Asp Phe Ser Met Leu Ala Ser Lys Tyr Pro Gly Ile Ile Thr
180 185 190
Pro Thr Lys Arg Asn Tyr Val Met Lys Ser Ala Tyr Tyr Met Asp Lys
195 200 205
Lys Glu His Thr Glu Lys Val Arg Gln Leu Met Asp Glu Ile Lys Ala
210 215 220
Ile Glu Pro Lys Pro Phe Glu Gly Lys Arg Val Ile Thr Thr Gly Ile
225 230 235 240
Ile Ala Asp Ser Glu Asp Leu Leu Lys Ile Leu Glu Glu Asn Asn Ile
245 250 255
Ala Ile Val Gly Asp Asp Ile Ala His Glu Ser Arg Gln Tyr Arg Thr
260 265 270
Leu Thr Pro Glu Ala Asn Thr Pro Met Asp Arg Leu Ala Glu Gln Phe
275 280 285
Ala Asn Arg Glu Cys Ser Thr Leu Tyr Asp Pro Glu Lys Lys Arg Gly
290 295 300
Gln Tyr Ile Val Glu Met Ala Lys Glu Arg Lys Ala Asp Gly Ile Ile
305 310 315 320
Phe Phe Met Thr Lys Phe Cys Asp Pro Glu Glu Tyr Asp Tyr Pro Gln
325 330 335
Met Lys Lys Asp Phe Glu Glu Ala Gly Ile Pro His Val Leu Ile Glu
340 345 350
Thr Asp Met Gln Met Lys Asn Tyr Glu Gln Ala Arg Thr Ala Ile Gln
355 360 365
Ala Phe Ser Glu Thr Leu
370
<210> 43
<211> 264
<212> PRT
<213> Clostridium sporogenes
<400> 43
Met Ala Asp Ile Tyr Thr Met Gly Val Asp Ile Gly Ser Thr Ala Ser
1 5 10 15
Lys Thr Val Val Leu Lys Asn Gly Lys Glu Ile Val Ser Gln Ala Val
20 25 30
Ile Ser Val Gly Ala Gly Thr Ser Gly Pro Lys Arg Ala Ile Asp Ser
35 40 45
Val Leu Lys Asp Ala Lys Leu Ser Ile Glu Asp Leu Asp Tyr Ile Val
50 55 60
Ser Thr Gly Tyr Gly Arg Asn Ser Phe Asp Phe Ala Asn Lys Gln Ile
65 70 75 80
Ser Glu Leu Ser Cys His Ala Lys Gly Val Tyr Phe Asp Asn Asn Lys
85 90 95
Ala Arg Thr Val Ile Asp Ile Gly Gly Gln Asp Ile Lys Val Leu Lys
100 105 110
Leu Ala Asp Ser Gly Arg Leu Leu Asn Phe Ile Met Asn Asp Lys Cys
115 120 125
Ala Ala Gly Thr Gly Arg Phe Leu Asp Val Met Ser Arg Val Ile Glu
130 135 140
Val Pro Val Asp Glu Leu Gly Lys Lys Ala Leu Glu Ser Lys Asn Pro
145 150 155 160
Cys Thr Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile
165 170 175
Ser Gln Leu Ala Arg Gly Val Lys Thr Glu Asp Leu Ile Ala Gly Ile
180 185 190
Cys Lys Ser Val Ala Ser Arg Val Ala Ser Leu Ala Lys Arg Ser Gly
195 200 205
Ile Glu Glu Leu Val Val Met Ser Gly Gly Val Ala Lys Asn Ile Gly
210 215 220
Val Val Lys Ala Met Glu Ala Glu Leu Gly Arg Asp Ile Tyr Ile Ser
225 230 235 240
Lys Asn Ser Gln Leu Asn Gly Ala Leu Gly Ala Ser Leu Tyr Ala Tyr
245 250 255
Glu Ser Phe Gln Lys Glu Arg Ser
260
<210> 44
<211> 412
<212> PRT
<213> Clostridium sporogenes
<400> 44
Met Glu Asn Asn Thr Asn Met Phe Ser Gly Val Lys Val Ile Glu Leu
1 5 10 15
Ala Asn Phe Ile Ala Ala Pro Ala Ala Gly Arg Phe Phe Ala Asp Gly
20 25 30
Gly Ala Glu Val Ile Lys Ile Glu Ser Pro Ala Gly Asp Pro Leu Arg
35 40 45
Tyr Thr Ala Pro Ser Glu Gly Arg Pro Leu Ser Gln Glu Glu Asn Thr
50 55 60
Thr Tyr Asp Leu Glu Asn Ala Asn Lys Lys Ala Ile Val Leu Asn Leu
65 70 75 80
Lys Ser Glu Lys Gly Lys Lys Ile Leu His Glu Met Leu Ala Glu Ala
85 90 95
Asp Ile Leu Leu Thr Asn Trp Arg Thr Lys Ala Leu Val Lys Gln Gly
100 105 110
Leu Asp Tyr Glu Thr Leu Lys Glu Lys Tyr Pro Lys Leu Val Phe Ala
115 120 125
Gln Ile Thr Gly Tyr Gly Glu Lys Gly Pro Asp Lys Asp Leu Pro Gly
130 135 140
Phe Asp Tyr Thr Ala Phe Phe Ala Arg Gly Gly Val Ser Gly Thr Leu
145 150 155 160
Tyr Glu Lys Gly Thr Val Pro Pro Asn Val Val Pro Gly Leu Gly Asp
165 170 175
His Gln Ala Gly Met Phe Leu Ala Ala Gly Met Ala Gly Ala Leu Tyr
180 185 190
Lys Ala Lys Thr Thr Gly Gln Gly Asp Lys Val Thr Val Ser Leu Met
195 200 205
His Ser Ala Met Tyr Gly Leu Gly Ile Met Ile Gln Ala Ala Gln Tyr
210 215 220
Lys Asp His Gly Leu Val Tyr Pro Ile Asn Arg Asn Glu Thr Pro Asn
225 230 235 240
Pro Phe Ile Val Ser Tyr Lys Ser Lys Asp Asp Tyr Phe Val Gln Val
245 250 255
Cys Met Pro Pro Tyr Asp Val Phe Tyr Asp Arg Phe Met Thr Ala Leu
260 265 270
Gly Arg Glu Asp Leu Val Gly Asp Glu Arg Tyr Asn Lys Ile Glu Asn
275 280 285
Leu Lys Asp Gly Arg Ala Lys Glu Val Tyr Ser Ile Ile Glu Gln Gln
290 295 300
Met Val Thr Lys Thr Lys Asp Glu Trp Asp Asn Ile Phe Arg Asp Ala
305 310 315 320
Asp Ile Pro Phe Ala Ile Ala Gln Thr Trp Glu Asp Leu Leu Glu Asp
325 330 335
Glu Gln Ala Trp Ala Asn Asp Tyr Leu Tyr Lys Met Lys Tyr Pro Thr
340 345 350
Gly Asn Glu Arg Ala Leu Val Arg Leu Pro Val Phe Phe Lys Glu Ala
355 360 365
Gly Leu Pro Glu Tyr Asn Gln Ser Pro Gln Ile Ala Glu Asn Thr Val
370 375 380
Glu Val Leu Lys Glu Met Gly Tyr Thr Glu Gln Glu Ile Glu Glu Leu
385 390 395 400
Glu Lys Asp Lys Asp Ile Met Val Arg Lys Glu Lys
405 410
<210> 45
<211> 368
<212> PRT
<213> Lachnoanaerobaculum saburreum
<400> 45
Met Trp His Cys Leu Glu Thr Leu Lys Lys Ile Ser Ala Ser Pro Lys
1 5 10 15
Glu Gln Leu Asn Lys Tyr Leu Glu Glu Gly Lys Lys Val Ile Ala Val
20 25 30
Ala Pro Val Tyr Thr Pro Glu Glu Ile Ile His Ala Phe Gly Phe Val
35 40 45
Pro Met Gly Val Trp Gly Ala Asp Ile Glu Ile Asn Glu Ser Lys Lys
50 55 60
Tyr Tyr Pro Ala Phe Ile Cys Ser Ile Met Gln Thr Val Leu Glu Leu
65 70 75 80
Gly Ile Lys Gly Asn Tyr Asn Gly Val Ser Ala Ile Val Val Pro Ser
85 90 95
Leu Cys Asp Ser Leu Lys Thr Leu Gly Gln Asn Trp Lys Tyr Ala Val
100 105 110
Lys Asp Ile Pro Phe Ile Pro Met Thr Tyr Pro Gln Asn Arg Lys Ser
115 120 125
Asp Tyr Ala Val Asp Phe Thr Leu Glu Met Tyr Lys Arg Val Ile Ser
130 135 140
Asp Leu Glu Asn Ile Thr Gly Glu Lys Phe Asp Glu Gly Lys Leu Lys
145 150 155 160
Asn Thr Tyr Glu Ile Tyr Asn Glu His Asn Arg Val Met Arg Glu Phe
165 170 175
Thr Lys Val Ser Glu Glu Tyr Glu Val Ser Ala Thr Asp Arg Ser Ala
180 185 190
Val Phe Lys Ser Ala Trp Phe Met Leu Lys Glu Glu His Thr Glu Leu
195 200 205
Val Arg Glu Leu Ile Glu Leu Ile Lys Lys Glu Gly Lys Ile Ser Lys
210 215 220
Lys Leu Arg Ile Tyr Thr Thr Gly Ile Leu Ala Asp Ala Pro Asp Leu
225 230 235 240
Leu Asn Ile Phe Asp Ser Asn Asn Met Gln Ile Val Gly Asp Asp Ile
245 250 255
Ala Tyr Glu Ser Arg Gln Tyr Arg Thr Asp Ile Pro Asp Gly Asn Gly
260 265 270
Leu Tyr Ala Leu Ala Lys Lys Phe Ser Asn Met Asp Asn Cys Thr Leu
275 280 285
Leu Tyr Asp Lys Asp Lys Arg Arg Val Asp Phe Ile Ile Glu Glu Ala
290 295 300
Lys Lys Lys Arg Ala Asp Gly Ile Val Val Leu Met Thr Lys Phe Cys
305 310 315 320
Asp Pro Glu Glu Phe Asp Tyr Val Pro Ile Lys Arg Ala Ala Asn Glu
325 330 335
Ala Gly Ile Pro His Ile Asn Ile Glu Val Asp Arg Gln Met Lys Asn
340 345 350
Tyr Gln Gln Ala Asn Thr Met Leu Gln Thr Phe Ala Asp Met Leu Val
355 360 365
<210> 46
<211> 409
<212> PRT
<213> Lachnoanaerobaculum saburreum
<400> 46
Met Glu Glu Ala Lys Lys Gln Lys Pro Thr Val Asp Pro Asn Ser Ala
1 5 10 15
Lys Ala Arg Leu Gly Arg Ile Ala Ala Lys Ala Tyr Ser Asp Cys Val
20 25 30
Glu Ala Lys Lys Arg Gly Glu Leu Val Gly Trp Cys Ala Ser Asn Phe
35 40 45
Pro Val Glu Ile Pro Glu Thr Leu Gly Leu Tyr Val Cys Tyr Pro Glu
50 55 60
Asn Gln Ala Ala Gly Ile Ala Ala Arg Gly Gly Gly Glu Arg Met Cys
65 70 75 80
Ser Glu Ser Glu Gly Asp Gly Tyr Ser Asn Asp Ile Cys Ala Tyr Ala
85 90 95
Arg Ile Ser Leu Ala Tyr Met Lys Leu Lys Glu Ala Pro Glu Gln Asp
100 105 110
Met Pro Gln Pro Asp Phe Val Leu Cys Cys Asn Asn Ile Cys Asn Cys
115 120 125
Met Ile Lys Trp Tyr Glu Asn Ile Ala Lys Glu Leu Asn Ile Pro Met
130 135 140
Ile Met Ile Asp Ile Pro Phe Asn Pro Asp Tyr Glu Val Ser Asp Ala
145 150 155 160
Met Thr Ala Tyr Ile Arg Asn Gln Phe Trp Asp Ala Ile His Gln Leu
165 170 175
Glu Glu Ile Thr Gly Lys Lys Trp Ser Asn Glu Arg Tyr Glu Glu Val
180 185 190
Arg Lys Ile Ser Gly Arg Ser Ser Arg Ala Trp Leu Glu Ala Thr Ala
195 200 205
Thr Ala Lys Tyr Ser Pro Ser Pro Phe Asn Gly Phe Asp Leu Leu Asn
210 215 220
His Met Ala Val Met Val Thr Ala Arg Gly Lys Leu Glu Ala Ala Glu
225 230 235 240
Ala Met Glu Thr Leu Leu Gln Glu Tyr Lys Asp Asn His Glu Lys Gly
245 250 255
Glu Ser Thr Phe Lys Gly Glu Glu Lys Tyr Arg Ile Met Phe Glu Gly
260 265 270
Ile Ala Cys Trp Pro Trp Leu Arg Ala Thr Ala Thr Gly Leu Lys Ser
275 280 285
Arg Gly Ile Asn Met Val Thr Thr Ile Tyr Ala Asp Ala Phe Gly Phe
290 295 300
Ile Tyr Asp Asp Phe Asp Gly Met Cys Arg Ala Tyr Ala Asn Val Pro
305 310 315 320
Asn Cys Met Asn Ile Glu His Ala Arg Asp Lys Arg Ile Lys Leu Cys
325 330 335
Lys Asp Asn Ser Val Glu Gly Leu Leu Val His Thr Asn Arg Ser Cys
340 345 350
Lys Leu Trp Ser Gly Phe Met Ser Glu Met Ser Arg Gln Ile Gly Glu
355 360 365
Glu Cys Gly Ile Pro Val Val Ser Phe Asp Gly Asp Gln Ala Asp Pro
370 375 380
Arg Asn Phe Ser Glu Ala Gln Tyr Asp Thr Arg Val Gln Gly Leu Thr
385 390 395 400
Glu Ile Met Glu Ala Asn Lys Glu Ile
405
<210> 47
<211> 256
<212> PRT
<213> Lachnoanaerobaculum saburreum
<400> 47
Met Tyr Thr Leu Gly Val Asp Ile Gly Ser Thr Thr Ser Lys Ala Val
1 5 10 15
Ile Leu Glu Asp Gly Glu Asn Ile Val Ala Ser Ser Ile Val Ile Ala
20 25 30
Thr Val Gly Thr Ala Gly Val Glu Glu Ala Val Lys Asn Val Leu Asn
35 40 45
Phe Ser Lys Leu Glu Leu Asn Asp Ile Lys Ala Val Val Ala Thr Gly
50 55 60
Tyr Gly Arg Met Asn Tyr Asp Val Ala Asp Tyr Lys Val Ser Glu Leu
65 70 75 80
Thr Cys His Ala Leu Gly Val His Lys Glu Phe Pro Asn Val Arg Thr
85 90 95
Val Ile Asp Ile Gly Gly Gln Asp Ala Lys Val Ile Ser Leu Ala Ala
100 105 110
Asn Gly Lys Met Thr Asn Phe Val Met Asn Asp Lys Cys Ala Ala Gly
115 120 125
Thr Gly Arg Phe Leu Asp Val Met Ala Asn Ile Leu Asn Leu Asp Ile
130 135 140
Gln Asp Leu Glu Val Glu Ala Leu Lys Ser Asp Asn Pro Ala Asn Ile
145 150 155 160
Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu
165 170 175
Ala Thr Gly Arg Asn Ile Pro Asp Leu Val Ala Gly Ile Cys Lys Ser
180 185 190
Val Ala Val Arg Val Ala Ala Leu Ala Lys Arg Val Gly Ile Val Glu
195 200 205
Glu Val Cys Met Ser Gly Gly Val Ala Lys Asn Ser Gly Val Arg Asn
210 215 220
Ala Met Ser Lys Glu Leu Gly Val Asp Ile Val Phe Ser Lys Asp Ala
225 230 235 240
Gln Leu Met Gly Ala Leu Gly Ala Ala Ile Tyr Gly Phe Lys Lys Leu
245 250 255
<210> 48
<211> 264
<212> PRT
<213> Peptostreptococcus stomatis
<400> 48
Met Ser Ser Val Tyr Thr Met Gly Ile Asp Ile Gly Ser Thr Ser Ser
1 5 10 15
Lys Cys Val Ile Met Lys Asp Gly Lys Glu Ile Val Ser Glu Gly Val
20 25 30
Val Ser Leu Gly Ala Gly Thr Lys Gly Ser Asp Leu Val Ile Glu Glu
35 40 45
Val Leu Gly Lys Ala Gly Met Thr Phe Asp Glu Ile Asp Leu Ile Val
50 55 60
Ser Thr Gly Tyr Gly Arg Asn Ser Tyr Glu Arg Ala Ala Lys Thr Val
65 70 75 80
Ser Glu Leu Ser Cys His Ala Lys Gly Gly Gly Tyr Ile Phe Gly Gly
85 90 95
Ala Gly Thr Ile Ile Asp Ile Gly Gly Gln Asp Ile Lys Val Leu Lys
100 105 110
Leu Asn Asp Lys Gly Gly Leu Val Asn Phe Leu Met Asn Asp Lys Cys
115 120 125
Ala Ala Gly Thr Gly Arg Phe Leu Glu Val Met Ser Gly Val Leu Asp
130 135 140
Val Lys Leu Asp Glu Leu Gly Glu Leu Asp Ala Lys Ala Thr Glu Val
145 150 155 160
Thr Pro Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile
165 170 175
Ser Cys Met Ala Lys Lys Ile Pro Leu Glu Asn Ile Ile Arg Gly Ile
180 185 190
His Ala Ser Val Ala Thr Arg Val Ala Ser Leu Ala Arg Arg Gly Gly
195 200 205
Leu Lys Thr Pro Val Ala Met Thr Gly Gly Val Ser Lys Asn Lys Gly
210 215 220
Ile Val Arg Ala Leu Lys Glu Glu Leu Glu Cys Asp Ile Leu Ile Ser
225 230 235 240
Pro Asp Ser Gln Met Ala Gly Ala Ile Gly Ala Ala Leu Tyr Ala Tyr
245 250 255
Asp Glu Tyr Gln Lys Gln Asn Ala
260
<210> 49
<211> 372
<212> PRT
<213> Peptostreptococcus stomatis
<400> 49
Met Ser Asn Ile Asp Val Leu Leu Gly Lys Leu Asp Val Ser Leu Leu
1 5 10 15
Gly Gln Val Asp Lys Tyr Val Ser Glu Gly Lys Lys Val Ile Gly Cys
20 25 30
Ala Pro Val Tyr Thr Pro Glu Glu Leu Val Tyr Ala Ala Gly Met Val
35 40 45
Pro Ile Gly Val Trp Gly Ala Glu Gly Glu Val Gly Leu Ser Lys Glu
50 55 60
Tyr Phe Pro Ala Phe Tyr Ala Ala Ile Ile Leu Arg Leu Met Asp Leu
65 70 75 80
Gly Leu Glu Gly Lys Leu Asp Lys Met Ser Gly Met Ile Ile Pro Gly
85 90 95
Leu Ser Asp Gly Leu Lys Gly Leu Ser Gln Asn Trp Lys Arg Ala Ile
100 105 110
Lys Gln Val Pro Ala Leu Tyr Ile Gly Tyr Gly Gln Asn Arg Lys Ile
115 120 125
Glu Ala Gly Ile Thr Tyr Asn Glu Lys Gln Tyr Ile Lys Leu Arg Gly
130 135 140
Gln Leu Glu Glu Ile Ala Gly Cys Lys Ile Glu Asp Ala Lys Val Glu
145 150 155 160
Glu Ala Ile Val Leu Tyr Asn Lys His Arg Lys Ala Met Gln Glu Phe
165 170 175
Ser Ser Leu Ala Ala Ser His Leu Asn Thr Ile Thr Pro Ile Leu Arg
180 185 190
Ala Arg Val Met Thr Ser Ala Phe Leu Phe Asp Lys Ala Glu His Leu
195 200 205
Ala Ile Leu Glu Glu Leu Asn Lys Glu Leu Lys Ala Leu Pro Glu Glu
210 215 220
Lys Phe Ala Gly Lys Lys Val Val Thr Thr Gly Ile Leu Ala Asn Ser
225 230 235 240
Pro Gly Met Leu Glu Ile Leu Asp Glu Tyr Lys Leu Gly Ile Val Asp
245 250 255
Asp Asn Ile Asn His Glu Ser Gly Gln Phe Asp Tyr Leu Val Asp Glu
260 265 270
Gly Thr Gly Asn Pro Val Arg Ala Leu Ser Lys Trp Ile Ser Asp Ile
275 280 285
Glu Gly Ser Thr Leu Leu Tyr Asp Pro Glu Lys Leu Arg Gly Gln Ile
290 295 300
Ile Ile Asp Lys Val Lys Lys His Gln Ala Asp Gly Val Ile Tyr Leu
305 310 315 320
Met Thr Lys Phe Ser Asp Ser Asp Glu Phe Asp Tyr Pro Ile Ile Arg
325 330 335
Lys Glu Leu Glu Asn Ala Gly Ile Leu His Ile Leu Val Glu Val Asp
340 345 350
Gln Gln Met Thr Asn Phe Glu Gln Ala Lys Thr Ala Leu Gln Thr Phe
355 360 365
Ala Asp Met Ile
370
<210> 50
<211> 411
<212> PRT
<213> Peptostreptococcus stomatis
<400> 50
Met Ser Asn Thr Gly Met Val Glu Glu Lys Pro Ala Lys Val Leu Leu
1 5 10 15
Gly Glu Ile Val Ala Lys His Tyr Lys Glu Ala Trp Glu Ala Lys Asn
20 25 30
Asn Gly Glu Leu Val Gly Trp Cys Ala Ser Asn Phe Pro Gln Glu Ile
35 40 45
Phe Glu Thr Met Asp Ile Lys Val Val Tyr Pro Glu Asn Gln Ala Ala
50 55 60
Ala Ile Ser Ala Lys Gly Gly Gly Gln Arg Met Cys Glu Ile Ala Glu
65 70 75 80
Asn Glu Gly Tyr Ser Asn Asp Ile Cys Ala Tyr Ala Arg Ile Ser Leu
85 90 95
Ala Tyr Met Asp Val Lys Asp Ala Pro Glu Leu Asn Met Pro Gln Pro
100 105 110
Asp Phe Val Ala Cys Cys Asn Asn Ile Cys Asn Cys Met Ile Lys Trp
115 120 125
Tyr Glu Asn Ile Ala Lys Glu Leu Asn Ile Pro Leu Ile Leu Ile Asp
130 135 140
Val Pro Tyr Asn Asn Asp Tyr Glu Ala Glu Asp Asp Arg Val Glu Tyr
145 150 155 160
Leu Arg Gly Gln Phe Asp Tyr Ala Ile Lys Gln Leu Glu Glu Leu Thr
165 170 175
Gly Lys Lys Trp Asp Glu Lys Lys Phe Glu Glu Val Met Glu Val Ser
180 185 190
Gln Arg Thr Gly Arg Ala Trp Leu Lys Ala Thr Gly Tyr Ala Lys Tyr
195 200 205
Thr Pro Ser Pro Phe Ser Gly Phe Asp Val Phe Asn His Met Ala Val
210 215 220
Ala Val Cys Ala Arg Gly Lys Ile Glu Ser Ala Ile Ala Phe Glu Lys
225 230 235 240
Leu Ala Glu Glu Phe Asp Glu Asn Val Arg Thr Gly Lys Ser Thr Phe
245 250 255
Lys Gly Glu Glu Lys Phe Arg Val Leu Phe Glu Gly Ile Ala Cys Trp
260 265 270
Pro His Leu Arg His Thr Phe Lys Gln Leu Lys Asp Ala Gly Val Asn
275 280 285
Val Cys Gly Thr Val Tyr Ala Asp Ala Phe Gly Tyr Ile Tyr Asp Asn
290 295 300
Thr Tyr Gln Leu Met Gln Ala Tyr Cys Gly Thr Pro Asn Ala Ile Ser
305 310 315 320
Tyr Glu Arg Ala Thr Asp Met Arg Leu Lys Val Ile Glu Glu Asn Asn
325 330 335
Ile Asp Gly Met Leu Ile His Ile Asn Arg Ser Cys Lys Gln Trp Ser
340 345 350
Gly Ile Met Tyr Glu Met Glu Arg Asp Ile Arg Glu Lys Thr Gly Ile
355 360 365
Pro Thr Ala Thr Phe Asp Gly Asp Gln Ala Asp Pro Arg Asn Phe Ser
370 375 380
Glu Ala Gln Tyr Asp Thr Arg Val Gln Gly Leu Ile Glu Leu Met Glu
385 390 395 400
Ala Asn Lys Ala Ala Lys Met Lys Glu Ala His
405 410
<210> 51
<211> 408
<212> PRT
<213> Clostridium difficile
<400> 51
Met Ser Glu Lys Lys Glu Ala Arg Val Val Ile Asn Asp Leu Leu Ala
1 5 10 15
Glu Gln Tyr Ala Asn Ala Phe Lys Ala Lys Glu Glu Gly Arg Pro Val
20 25 30
Gly Trp Ser Thr Ser Val Phe Pro Gln Glu Leu Ala Glu Val Phe Asp
35 40 45
Leu Asn Val Leu Tyr Pro Glu Asn Gln Ala Ala Gly Val Ala Ala Lys
50 55 60
Lys Gly Ser Leu Glu Leu Cys Glu Ile Ala Glu Ser Lys Gly Tyr Ser
65 70 75 80
Ile Asp Leu Cys Ala Tyr Ala Arg Thr Asn Phe Gly Leu Leu Glu Asn
85 90 95
Gly Gly Cys Glu Ala Leu Asp Met Pro Ala Pro Asp Phe Leu Leu Cys
100 105 110
Cys Asn Asn Ile Cys Asn Gln Val Ile Lys Trp Tyr Glu Asn Ile Ser
115 120 125
Arg Glu Leu Asp Ile Pro Leu Ile Met Ile Asp Thr Thr Phe Asn Asn
130 135 140
Glu Asp Glu Val Thr Gln Ser Arg Ile Asp Tyr Ile Lys Ala Gln Phe
145 150 155 160
Glu Glu Ala Ile Lys Gln Leu Glu Ile Ile Ser Gly Lys Lys Phe Asp
165 170 175
Pro Lys Lys Phe Glu Glu Val Met Lys Ile Ser Ala Glu Asn Gly Arg
180 185 190
Leu Trp Lys Tyr Ser Met Ser Leu Pro Ala Asp Ser Ser Pro Ser Pro
195 200 205
Met Asn Gly Phe Asp Leu Phe Thr Tyr Met Ala Val Ile Val Cys Ala
210 215 220
Arg Gly Lys Lys Glu Thr Thr Glu Ala Phe Lys Leu Leu Ile Glu Glu
225 230 235 240
Leu Glu Asp Asn Met Lys Thr Gly Lys Ser Ser Phe Arg Gly Glu Glu
245 250 255
Lys Tyr Arg Ile Met Met Glu Gly Ile Pro Cys Trp Pro Tyr Ile Gly
260 265 270
Tyr Lys Met Lys Thr Leu Ala Lys Phe Gly Val Asn Met Thr Gly Ser
275 280 285
Val Tyr Pro His Ala Trp Ala Leu Gln Tyr Glu Val Asn Asp Leu Asp
290 295 300
Gly Met Ala Val Ala Tyr Ser Thr Met Phe Asn Asn Val Asn Leu Asp
305 310 315 320
Arg Met Thr Lys Tyr Arg Val Asp Ser Leu Val Glu Gly Lys Cys Asp
325 330 335
Gly Ala Phe Tyr His Met Asn Arg Ser Cys Lys Leu Met Ser Leu Ile
340 345 350
Gln Tyr Glu Met Gln Arg Arg Ala Ala Glu Glu Thr Gly Leu Pro Tyr
355 360 365
Ala Gly Phe Asp Gly Asp Gln Ala Asp Pro Arg Ala Phe Thr Asn Ala
370 375 380
Gln Phe Glu Thr Arg Ile Gln Gly Leu Val Glu Val Met Glu Glu Arg
385 390 395 400
Lys Lys Leu Asn Arg Gly Glu Ile
405
<210> 52
<211> 375
<212> PRT
<213> Clostridium difficile
<400> 52
Met Glu Ala Ile Leu Ser Lys Met Lys Glu Val Val Glu Asn Pro Asn
1 5 10 15
Ala Ala Val Lys Lys Tyr Lys Ser Glu Thr Gly Lys Lys Ala Ile Gly
20 25 30
Cys Phe Pro Val Tyr Cys Pro Glu Glu Ile Ile His Ala Ala Gly Met
35 40 45
Leu Pro Val Gly Ile Trp Gly Gly Gln Thr Glu Leu Asp Leu Ala Lys
50 55 60
Gln Tyr Phe Pro Ala Phe Ala Cys Ser Ile Met Gln Ser Cys Leu Glu
65 70 75 80
Tyr Gly Leu Lys Gly Ala Tyr Asp Glu Leu Ser Gly Val Ile Ile Pro
85 90 95
Gly Met Cys Asp Thr Leu Ile Cys Leu Gly Gln Asn Trp Lys Ser Ala
100 105 110
Val Pro His Ile Lys Tyr Ile Ser Leu Val His Pro Gln Asn Arg Lys
115 120 125
Leu Glu Ala Gly Val Lys Tyr Leu Ile Ser Glu Tyr Lys Gly Val Lys
130 135 140
Arg Glu Leu Glu Glu Ile Cys Gly Tyr Glu Ile Glu Glu Ala Lys Ile
145 150 155 160
His Glu Ser Ile Glu Val Tyr Asn Glu His Arg Lys Thr Met Arg Asp
165 170 175
Phe Val Glu Val Ala Tyr Lys His Ser Asn Thr Ile Lys Pro Ser Ile
180 185 190
Arg Ser Leu Val Ile Lys Ser Gly Phe Phe Met Arg Lys Glu Glu His
195 200 205
Thr Glu Leu Val Lys Asp Leu Ile Ala Lys Leu Asn Ala Met Pro Glu
210 215 220
Glu Val Cys Ser Gly Lys Lys Val Leu Leu Thr Gly Ile Leu Ala Asp
225 230 235 240
Ser Lys Asp Ile Leu Asp Ile Leu Glu Asp Asn Asn Ile Ser Val Val
245 250 255
Ala Asp Asp Leu Ala Gln Glu Thr Arg Gln Phe Arg Thr Asp Val Pro
260 265 270
Ala Gly Asp Asp Ala Leu Glu Arg Leu Ala Arg Gln Trp Ser Asn Ile
275 280 285
Glu Gly Cys Ser Leu Ala Tyr Asp Pro Lys Lys Lys Arg Gly Ser Leu
290 295 300
Ile Val Asp Glu Val Lys Lys Lys Asp Ile Asp Gly Val Ile Phe Cys
305 310 315 320
Met Met Lys Phe Cys Asp Pro Glu Glu Tyr Asp Tyr Pro Leu Val Arg
325 330 335
Lys Asp Ile Glu Asp Ser Gly Ile Pro Thr Leu Tyr Val Glu Ile Asp
340 345 350
Gln Gln Thr Gln Asn Asn Glu Gln Ala Arg Thr Arg Ile Gln Thr Phe
355 360 365
Ala Glu Met Met Ser Leu Ala
370 375
<210> 53
<211> 266
<212> PRT
<213> Clostridium difficile
<400> 53
Met Tyr Thr Met Gly Leu Asp Ile Gly Ser Thr Ala Ser Lys Gly Val
1 5 10 15
Ile Leu Lys Asn Gly Glu Asp Ile Val Ala Ser Glu Thr Ile Ser Ser
20 25 30
Gly Thr Gly Thr Thr Gly Pro Ser Arg Val Leu Glu Lys Leu Tyr Gly
35 40 45
Lys Thr Gly Leu Ala Arg Glu Asp Ile Lys Lys Val Val Val Thr Gly
50 55 60
Tyr Gly Arg Met Asn Tyr Ser Asp Ala Asp Lys Gln Ile Ser Glu Leu
65 70 75 80
Ser Cys His Ala Arg Gly Val Asn Phe Ile Ile Pro Glu Thr Arg Thr
85 90 95
Ile Ile Asp Ile Gly Gly Gln Asp Ala Lys Val Leu Lys Leu Asp Asn
100 105 110
Asn Gly Arg Leu Leu Asn Phe Leu Met Asn Asp Lys Cys Ala Ala Gly
115 120 125
Thr Gly Arg Phe Leu Asp Val Met Ala Lys Ile Ile Glu Val Asp Val
130 135 140
Ser Glu Leu Gly Ser Ile Ser Met Asn Ser Gln Asn Glu Val Ser Ile
145 150 155 160
Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser His Leu
165 170 175
Ser Glu Asn Ala Lys Ile Glu Asp Ile Val Ala Gly Ile His Thr Ser
180 185 190
Val Ala Lys Arg Val Ser Ser Leu Val Lys Arg Ile Gly Val Gln Arg
195 200 205
Asn Val Val Met Val Gly Gly Val Ala Arg Asn Ser Gly Ile Val Arg
210 215 220
Ala Met Ala Arg Glu Ile Asn Thr Glu Ile Ile Val Pro Asp Ile Pro
225 230 235 240
Gln Leu Thr Gly Ala Leu Gly Ala Ala Leu Tyr Ala Phe Asp Glu Ala
245 250 255
Lys Glu Ser Gln Lys Glu Val Lys Asn Ile
260 265
<210> 54
<211> 399
<212> PRT
<213> Clostridium difficile
<400> 54
Met Leu Leu Glu Gly Val Lys Val Val Glu Leu Ser Ser Phe Ile Ala
1 5 10 15
Ala Pro Cys Cys Ala Lys Met Leu Gly Asp Trp Gly Ala Glu Val Ile
20 25 30
Lys Ile Glu Pro Ile Glu Gly Asp Gly Ile Arg Val Met Gly Gly Thr
35 40 45
Phe Lys Ser Pro Ala Ser Asp Asp Glu Asn Pro Met Phe Glu Leu Glu
50 55 60
Asn Gly Asn Lys Lys Gly Val Ser Ile Asn Val Lys Ser Lys Glu Gly
65 70 75 80
Val Glu Ile Leu His Lys Leu Leu Ser Glu Ala Asp Ile Phe Val Thr
85 90 95
Asn Val Arg Val Gln Ala Leu Glu Lys Met Gly Ile Ala Tyr Asp Gln
100 105 110
Ile Lys Asp Lys Tyr Pro Gly Leu Ile Phe Ser Gln Ile Leu Gly Tyr
115 120 125
Gly Glu Lys Gly Pro Leu Lys Asp Lys Pro Gly Phe Asp Tyr Thr Ala
130 135 140
Tyr Phe Ala Arg Gly Gly Val Ser Gln Ser Val Met Glu Lys Gly Thr
145 150 155 160
Ser Pro Ala Asn Thr Ala Ala Gly Phe Gly Asp His Tyr Ala Gly Leu
165 170 175
Ala Leu Ala Ala Gly Ser Leu Ala Ala Leu His Lys Lys Ala Gln Thr
180 185 190
Gly Lys Gly Glu Arg Val Thr Val Ser Leu Phe His Thr Ala Ile Tyr
195 200 205
Gly Met Gly Thr Met Ile Thr Thr Ala Gln Tyr Gly Asn Glu Met Pro
210 215 220
Leu Ser Arg Glu Asn Pro Asn Ser Pro Leu Met Thr Thr Tyr Lys Cys
225 230 235 240
Lys Asp Gly Arg Trp Ile Gln Leu Ala Leu Ile Gln Tyr Asn Lys Trp
245 250 255
Leu Gly Lys Phe Cys Lys Val Ile Asn Arg Glu Tyr Ile Leu Glu Asp
260 265 270
Asp Arg Tyr Asn Asn Ile Asp Ser Met Val Asn His Val Glu Asp Leu
275 280 285
Val Lys Ile Val Gly Glu Ala Met Leu Glu Lys Thr Leu Asp Glu Trp
290 295 300
Ser Ala Leu Leu Glu Glu Ala Asp Leu Pro Phe Glu Lys Ile Gln Ser
305 310 315 320
Cys Glu Asp Leu Leu Asp Asp Glu Gln Ala Trp Ala Asn Asp Phe Leu
325 330 335
Phe Lys Lys Thr Tyr Asp Ser Gly Asn Thr Gly Val Leu Val Asn Thr
340 345 350
Pro Val Met Phe Arg Asn Glu Gly Ile Lys Glu Tyr Thr Pro Ala Pro
355 360 365
Lys Val Gly Gln His Thr Val Glu Val Leu Lys Ser Leu Gly Tyr Asp
370 375 380
Glu Glu Lys Ile Asn Asn Phe Lys Asp Ser Lys Val Val Arg Tyr
385 390 395
<210> 55
<211> 255
<212> PRT
<213> Escherichia coli (strain K12)
<400> 55
Met Ser Glu Leu Ile Val Ser Arg Gln Gln Arg Val Leu Leu Leu Thr
1 5 10 15
Leu Asn Arg Pro Ala Ala Arg Asn Ala Leu Asn Asn Ala Leu Leu Thr
20 25 30
Gln Leu Val Asn Glu Leu Glu Ala Ala Ala Ile Asp Thr Ser Ile Ser
35 40 45
Val Cys Val Ile Thr Gly Asn Ala Arg Phe Phe Ala Ala Gly Ala Asp
50 55 60
Leu Asn Glu Met Ala Glu Lys Asp Leu Ala Ala Thr Leu Asn Asp Thr
65 70 75 80
Arg Pro Gln Leu Trp Ala Arg Leu Gln Ala Phe Asn Lys Pro Leu Ile
85 90 95
Ala Ala Val Asn Gly Tyr Ala Leu Gly Ala Gly Cys Glu Leu Ala Leu
100 105 110
Leu Cys Asp Val Val Val Ala Gly Glu Asn Ala Arg Phe Gly Leu Pro
115 120 125
Glu Ile Thr Leu Gly Ile Met Pro Gly Ala Gly Gly Thr Gln Arg Leu
130 135 140
Ile Arg Ser Val Gly Lys Ser Leu Ala Ser Lys Met Val Leu Ser Gly
145 150 155 160
Glu Ser Ile Thr Ala Arg Gln Ala Gln Gln Ala Gly Leu Val Ser Asp
165 170 175
Val Phe Pro Ser Asp Leu Thr Leu Glu Tyr Ala Leu Gln Leu Ala Ser
180 185 190
Lys Met Ala Arg His Ser Pro Leu Ala Leu Gln Ala Ala Lys Gln Ala
195 200 205
Leu Arg Gln Ser Gln Glu Val Ala Leu Gln Ala Gly Leu Ala Gln Glu
210 215 220
Arg Gln Leu Phe Thr Leu Leu Ala Ala Thr Glu Asp Arg His Glu Gly
225 230 235 240
Ile Ser Ala Phe Leu Gln Lys Arg Ser Pro Asp Phe Lys Gly Arg
245 250 255
<210> 56
<211> 257
<212> PRT
<213> Rhodobacter capsulatus
<400> 56
Met Ser Tyr His Thr Ile Arg Tyr Glu Ile Ser Glu Gly Leu Ala Val
1 5 10 15
Ile Thr Leu Asp Arg Pro Glu Val Met Asn Ala Leu Asn Ala Ala Met
20 25 30
Arg His Glu Leu Thr Ala Ala Leu His Arg Ala Arg Gly Glu Ala Arg
35 40 45
Ala Ile Val Leu Thr Gly Ser Gly Arg Ala Phe Cys Ser Gly Gln Asp
50 55 60
Leu Gly Asp Gly Ala Ala Glu Gly Leu Asn Leu Glu Thr Val Leu Arg
65 70 75 80
Glu Glu Tyr Glu Pro Leu Leu Gln Ala Ile Tyr Ser Cys Pro Leu Pro
85 90 95
Val Leu Ala Ala Val Asn Gly Ala Ala Ala Gly Ala Gly Ala Asn Leu
100 105 110
Ala Leu Ala Ala Asp Val Val Ile Ala Ala Gln Ser Ala Ala Phe Met
115 120 125
Gln Ala Phe Thr Arg Ile Gly Leu Met Pro Asp Ala Gly Gly Thr Trp
130 135 140
Trp Leu Pro Arg Gln Val Gly Met Ala Arg Ala Met Gly Met Ala Leu
145 150 155 160
Phe Ala Glu Lys Ile Gly Ala Glu Glu Ala Ala Arg Met Gly Leu Ile
165 170 175
Trp Glu Ala Val Pro Asp Val Asp Phe Glu His His Trp Arg Ala Arg
180 185 190
Ala Ala His Leu Ala Arg Gly Pro Ser Ala Ala Phe Ala Ala Val Lys
195 200 205
Lys Ala Phe His Ala Gly Leu Ser Asn Pro Leu Pro Ala Gln Leu Ala
210 215 220
Leu Glu Ala Arg Leu Gln Gly Glu Leu Gly Gln Ser Ala Asp Phe Arg
225 230 235 240
Glu Gly Val Gln Ala Phe Leu Glu Lys Arg Pro Pro His Phe Thr Gly
245 250 255
Arg
<210> 57
<211> 701
<212> PRT
<213> Pseudomonas stutzeri
<400> 57
Met Thr Asp Val Ile Arg Leu Glu Arg Arg Gly Asp Ile Ala Leu Ile
1 5 10 15
Leu Val Asn Asn Pro Pro Val Asn Ala Leu Gly His Ala Val Arg Lys
20 25 30
Gly Leu Leu Asp Ala Phe Gln Glu Ala Asp Glu Ala Pro Glu Val Thr
35 40 45
Ala Val Val Leu Val Cys Glu Gly Pro Thr Phe Met Ala Gly Ala Asp
50 55 60
Ile Lys Glu Phe Gly Lys Pro Pro Gln Ala Pro Ser Leu Pro Glu Val
65 70 75 80
Ile Glu Val Ile Glu Gly Cys Arg Lys Pro Ser Val Ala Val Ile His
85 90 95
Gly Thr Ala Leu Gly Gly Gly Leu Glu Val Ala Leu Gly Cys His Tyr
100 105 110
Arg Ile Ala Arg Ser Asp Ala Lys Val Gly Leu Pro Glu Val Lys Leu
115 120 125
Gly Leu Leu Pro Gly Ala Gly Gly Thr Gln Arg Leu Pro Arg Leu Ala
130 135 140
Gly Val Glu Lys Ala Leu Glu Met Ile Val Ser Gly Gln Pro Ile Gly
145 150 155 160
Ala Ala Glu Ala Leu Glu His Tyr Ile Val Asp Glu Leu Phe Glu Gly
165 170 175
Asp Leu Ile Glu Ala Gly Leu Thr Tyr Ala Arg Arg Leu Val Glu Glu
180 185 190
Gly Arg Gly Pro Arg Arg Ser Gly Glu Gln Thr Arg Gly Leu Glu Gly
195 200 205
Val Asp Asn Glu Ala Leu Ile Arg Ala Lys His Ala Glu Val Ala Lys
210 215 220
Arg Met Pro Gly Leu Phe Ser Pro Leu Arg Cys Ile Ala Ala Val Glu
225 230 235 240
Ala Ala Thr Arg Leu Pro Leu Ala Glu Gly Leu Lys Arg Glu Arg Glu
245 250 255
Leu Phe Thr Glu Cys Leu Asn Ser Pro Gln Arg Gly Ala Leu Ile His
260 265 270
Ser Phe Phe Ala Glu Arg Gln Ala Gly Lys Ile Asp Asp Leu Pro Ser
275 280 285
Asp Val Thr Pro Arg Pro Ile Arg Thr Ala Ala Val Ile Gly Gly Gly
290 295 300
Thr Met Gly Val Gly Ile Ala Leu Ser Phe Ala Asn Ala Gly Val Pro
305 310 315 320
Val Lys Leu Leu Glu Ile Asn Asp Glu Ala Leu Gln Arg Gly Leu Gln
325 330 335
Arg Ala Arg Glu Thr Tyr Ala Ala Ser Val Lys Arg Gly Ser Leu Thr
340 345 350
Glu Asp Ala Met Glu Gln Arg Leu Ala Leu Ile Ala Gly Val Thr Asp
355 360 365
Tyr Gly Ala Leu Ala Asp Ala Asp Val Val Val Glu Ala Val Phe Glu
370 375 380
Glu Met Gly Val Lys Gln Gln Val Phe Glu Gln Leu Asp Ala Val Cys
385 390 395 400
Lys Pro Gly Ala Ile Leu Ala Ser Asn Thr Ser Ser Leu Asp Leu Asn
405 410 415
Ala Ile Ala Gly Phe Thr Arg Arg Pro Glu Asp Val Val Gly Met His
420 425 430
Phe Phe Ser Pro Ala Asn Val Met Arg Leu Leu Glu Val Val Arg Gly
435 440 445
Glu Arg Thr Ser Asp Glu Val Leu Ala Ala Ala Met Ala Ile Gly Lys
450 455 460
Gln Leu Lys Lys Val Ser Val Val Val Gly Val Cys Asp Gly Phe Val
465 470 475 480
Gly Asn Arg Met Val Phe Gln Tyr Gly Arg Glu Ala Glu Phe Leu Leu
485 490 495
Glu Glu Gly Ala Thr Pro Gln Gln Val Asp Ala Ala Leu Arg Asn Phe
500 505 510
Gly Met Ala Met Gly Pro Phe Ala Met Arg Asp Leu Ser Gly Leu Asp
515 520 525
Ile Gly Gln Ala Ile Arg Lys Arg Gln Arg Ala Thr Leu Pro Ala His
530 535 540
Leu Asp Phe Pro Thr Val Ser Asp Lys Leu Cys Ala Ala Gly Met Leu
545 550 555 560
Gly Gln Lys Thr Gly Ala Gly Tyr Tyr Arg Tyr Glu Pro Gly Asn Arg
565 570 575
Thr Pro Gln Glu Asn Pro Asp Leu Ala Pro Met Leu Glu Ala Ala Ser
580 585 590
Arg Glu Lys Gly Ile Glu Arg Gln Ala Leu Asp Glu Gln Tyr Ile Val
595 600 605
Glu Arg Cys Ile Phe Ala Leu Val Asn Glu Gly Ala Lys Ile Leu Glu
610 615 620
Glu Gly Ile Ala Gln Arg Ser Ser Asp Ile Asp Val Ile Tyr Leu Asn
625 630 635 640
Gly Tyr Gly Phe Pro Ala Phe Arg Gly Gly Pro Met Tyr Tyr Ala Asp
645 650 655
Ser Val Gly Leu Asp Lys Val Leu Ala Arg Val Lys Glu Leu His Ala
660 665 670
Arg Cys Gly Asp Trp Trp Lys Pro Ala Pro Leu Leu Glu Lys Leu Ala
675 680 685
Ala Glu Gly Arg Thr Phe Thr Glu Trp Gln Ala Gly Gln
690 695 700
<210> 58
<211> 655
<212> PRT
<213> Haliangium ochraceum
<400> 58
Met Ile Val Gly Val Ile Gly Ser Gly Ala Ile Gly Pro Asp Leu Ala
1 5 10 15
Tyr Gly Phe Ala Ser Ala Leu Ala Ser Val Pro Gly Ala Arg Val Tyr
20 25 30
Leu His Asp Ile Lys Gln Glu Ala Leu Asp Ala Gly Met Gln Arg Ile
35 40 45
Arg Gly Tyr Ile Ala Lys Gly Leu Ala Arg Gly Lys Ile Ser Glu Arg
50 55 60
Val Ala Gly Ala Leu Glu Thr Val Leu Val Pro Thr Leu Ser Leu Ala
65 70 75 80
Asp Leu Ala Pro Cys Ser Tyr Val Leu Glu Ala Ala Thr Glu Glu Leu
85 90 95
Gly Val Lys Arg Ala Ile Leu Arg Ser Leu Glu Asp Thr Val Asp Ser
100 105 110
Glu Cys Leu Ile Gly Phe Ala Thr Ser Gly Leu Pro Arg Ala Ile Ile
115 120 125
Ala Ala Glu Val Lys His Pro Glu Arg Cys Phe Val Asn His Pro Phe
130 135 140
Tyr Pro Ala Trp Arg Ser Leu Pro Val Glu Val Val Leu Ser Gly Ser
145 150 155 160
Pro Ala His Gly Gln Arg Met Leu Ala Thr Leu Glu Ala Leu Gly Lys
165 170 175
Val Pro Val Ile Thr Ala Asp Ala Pro Cys Phe Ala Ala Asp Asp Ile
180 185 190
Phe Cys Asn Tyr Cys Ser Glu Ala Ala Arg Ile Val Glu Glu Gly Ile
195 200 205
Ala Asn Pro Ala Gln Val Asp Ala Ile Val His Gly Ala Ile Gly Gly
210 215 220
Gly Gly Pro Leu Asn Val Leu Asp Ala Thr Arg Gly Asn Leu Leu Thr
225 230 235 240
Val His Cys Gln Glu Leu Met Arg Asp Ala Asp Thr Gly Thr Pro Trp
245 250 255
Phe Glu Pro Pro Ala Ile Leu Arg Glu Arg Gly Asp Ala Leu Trp His
260 265 270
Asp Pro Lys Ala Pro His Asp Pro Ala Phe Asp Glu Ala Leu Arg Glu
275 280 285
Arg Val Leu Asp Arg Ile Leu Ala Val Leu Leu Ala Arg Thr Val Phe
290 295 300
Val Leu Asp His Gly Ile Cys Ala Ala Thr Glu Leu Asp Trp Met Thr
305 310 315 320
Arg Thr Ala Leu Gly Phe Arg Thr Gly Leu Val Asp Leu Val Asp Glu
325 330 335
Leu Gly Pro Glu Arg Val Ala Glu Leu Cys Gln Arg Tyr Ala Ala Glu
340 345 350
His Pro Gly Phe Val Ile Pro Asp Ser Ile Arg Glu Gln His Lys Pro
355 360 365
Arg Phe Tyr Gly Asn Leu Arg Val Thr Arg Gln Asp Glu Leu Ala Ile
370 375 380
Val Arg Ile Phe Arg Pro Glu Val Lys Asn Ala Leu Asp Arg Arg Thr
385 390 395 400
Leu Ser Glu Leu Asp His Leu Met Ala Ala Leu Ser Ala Asp Asp Ser
405 410 415
Val Glu Gly Val Val Leu Ser Ser Ala Gly Gly Ala Leu Ala Gly Ala
420 425 430
Asp Ile Thr Glu Leu Ala Arg Val Arg Thr Thr Glu Glu Ala Val Ser
435 440 445
Thr Cys Ala Phe Gly Gln Ala Val Leu Asn Arg Ile Ala Ala Met Asp
450 455 460
Lys Pro Val Val Ala Ala Val Asp Gly Pro Val Leu Gly Gly Gly Ala
465 470 475 480
Glu Leu Ser Met Ala Cys His Ala Arg Val Val Gly Pro Arg Leu Ser
485 490 495
Met Gly Gln Pro Glu Val Asn Leu Gly Ile Ile Pro Gly Tyr Gly Gly
500 505 510
Thr Gln Arg Leu Pro Arg Leu Ile Gly Val Glu Arg Ala Leu Ala Met
515 520 525
Met Arg Thr Ala Gln Ser Ile Asp Ala Gln Thr Ala Cys Glu Trp Gly
530 535 540
Trp Ala Ser Gly Thr Pro Met Val Asp Phe Val Gly Ala Ala Ala Thr
545 550 555 560
Leu Ile Arg Ser His Leu Ala Gly Glu Ala Glu Leu Ala Pro Leu Asp
565 570 575
Pro Ala Pro Met Ser Val Pro Ala Ala Ala Ala Pro Val Asp Ile Gly
580 585 590
His Arg Ser Arg Val Ile Asp Glu Ile Leu Val Asp Val Val Gln Ser
595 600 605
Gly Leu Arg Ala Pro Leu Ser Glu Gly Leu Ala Thr Glu Ala Ala Gly
610 615 620
Phe Gly Arg Cys Val Leu Thr Val Asp Leu Asp Ile Gly Leu Lys Asn
625 630 635 640
Phe Met Gln Asn Gly Pro Arg Val Pro Ala Leu Phe Leu His Glu
645 650 655
<210> 59
<211> 255
<212> PRT
<213> Anoxybacillus flavithermus
<400> 59
Met Phe Ser Ile Gln Gln Glu Gly Tyr Val Ala Ile Leu Ala Leu His
1 5 10 15
Arg Pro Pro Ala Asn Ala Leu Ala Ser Ser Val Leu Lys Glu Leu Ser
20 25 30
Glu Arg Leu Asp Ala Leu Lys Glu Asp Glu Gln Val Arg Val Ile Val
35 40 45
Leu His Gly Glu Gly Arg Phe Phe Ser Ala Gly Ala Asp Ile Lys Glu
50 55 60
Phe Thr Ala Ile Glu Ala Ser Glu Gln Ala Ala Glu Leu Ala Arg Ala
65 70 75 80
Gly Gln Gln Val Met Glu Lys Ile Glu Gln Phe Pro Lys Pro Ile Ile
85 90 95
Ala Ala Ile His Gly Ala Ala Leu Gly Gly Gly Leu Glu Leu Ala Met
100 105 110
Ser Cys His Leu Arg Ile Val Ala Glu Asn Ala Lys Leu Gly Leu Pro
115 120 125
Glu Leu Gln Leu Gly Ile Ile Pro Gly Phe Ala Gly Thr Gln Arg Leu
130 135 140
Leu Arg His Val Gly Met Ala Lys Ala Leu Glu Met Met Trp Thr Ser
145 150 155 160
Glu Pro Ile Thr Gly Ala Glu Ala Val Gln Trp Gly Leu Ala Asn Lys
165 170 175
Ala Val Pro Glu Glu Gln Leu Leu Asp Thr Ala Lys Gln Leu Ala Gln
180 185 190
Lys Ile Ala Gln Lys Ser Pro Ile Ser Val Gln Ala Val Leu Lys Leu
195 200 205
Val Asn Glu Ala Arg Thr Lys Thr Phe His Glu Cys Val Glu Lys Glu
210 215 220
Ala Gln Leu Phe Gly Gln Val Phe Val Thr Glu Asp Ala Lys Glu Gly
225 230 235 240
Ile Ser Ala Phe Ile Glu Lys Arg Thr Pro Gln Phe Gln Gly Lys
245 250 255
<210> 60
<211> 260
<212> PRT
<213> Streptomyces avermitilis
<400> 60
Met Ser Thr Ala Pro Glu Ala Ala Asp Leu Val Leu His Glu Arg His
1 5 10 15
Gly Gly Val Leu Thr Ile Thr Ile Asn Arg Pro Ala Gln Lys Asn Ala
20 25 30
Val Asp His Glu Ala Ala Val Gln Leu Ala Ala Ala Val Asp Leu Leu
35 40 45
Asp Ala Asp Pro Glu Leu Ser Val Gly Val Leu Thr Gly Ala Gly Gly
50 55 60
Val Phe Ser Ala Gly Met Asp Leu Lys Ala Phe Ala Lys Gly Glu Leu
65 70 75 80
Pro Leu Leu Pro Ser Arg Gly Leu Gly Gly Leu Thr Arg Ala Ser Val
85 90 95
Arg Lys Pro Leu Val Ala Ala Val Glu Gly Trp Ala Leu Gly Gly Gly
100 105 110
Phe Glu Leu Val Leu Ala Cys Asp Leu Ile Val Ala Ala Glu Asp Ala
115 120 125
Arg Phe Gly Phe Pro Glu Val Met Arg Gly Leu Val Ala Ala Glu Gly
130 135 140
Gly Leu Val Arg Leu Pro Arg Arg Leu Pro Tyr His Val Ala Ala Arg
145 150 155 160
Val Leu Leu Thr Gly Glu Pro Leu Thr Ala Val Glu Ala Lys Glu Tyr
165 170 175
Gly Leu Val Asn Glu Leu Thr Pro Pro Gly Ala Ala Leu Asp Ala Ala
180 185 190
Arg Glu Leu Ala Gly Arg Val Ala Arg Asn Ala Pro Leu Ala Leu Ala
195 200 205
Ala Val Lys Glu Val Leu Arg Glu Thr Gln Gly Leu Lys Glu Ser Asp
210 215 220
Ala Phe Arg Arg Gln Asp Glu Leu Thr Ser Gly Leu Ala Ala Ser Glu
225 230 235 240
Asp Ala Arg Glu Gly Ala Gln Ala Phe Ala Glu Lys Arg Ala Pro Val
245 250 255
Trp His Gly Arg
260
<210> 61
<211> 560
<212> PRT
<213> Advenella kashmirensis
<400> 61
Met Asp Asn Gly Arg Lys Leu Ile Glu Arg Gly Trp His Leu Phe Asn
1 5 10 15
Arg Ile Glu Lys Leu Ala Phe Pro Thr Leu Ala Leu Met His Gly Pro
20 25 30
Cys Leu Gly Gly Gly Leu Glu Leu Ala Leu Ala Cys Arg Tyr Arg Ile
35 40 45
Ala Ile Asp Ser Pro Lys Pro Val Ile Gly Leu Pro Glu Val Lys Leu
50 55 60
Gly Ile Phe Pro Ala Trp Gly Gly Leu Met Arg Leu Pro Arg Leu Ile
65 70 75 80
Gly Pro Gln Thr Ala Leu Asn Met Met Leu Thr Gly Arg Thr Leu Asp
85 90 95
Gly Arg Lys Ala Arg Ser Ala Gly Leu Val Asp Leu Leu Val Ala Pro
100 105 110
Arg Val Ala Glu Lys Ser Ala Ile Asp Leu Val Thr Ser Gly Lys Pro
115 120 125
Ala Arg Gln Ala Arg Gly Leu Ala Gly Leu Leu Asn Arg Ala Pro Phe
130 135 140
Lys Ser Leu Val Ala Ala Gln Ala Arg Lys Ser Val Lys Gln Lys Asp
145 150 155 160
Pro Tyr Gly His Tyr Pro Ala Thr Leu Thr Met Leu Asp Leu Trp Glu
165 170 175
Lys His Asp Gly Asp Pro Leu Ala Asp Pro Gln Ala Leu Thr Arg Leu
180 185 190
Leu Gln Ser Asp Val Thr Arg Asn Leu Ile Arg Val Phe His Leu Gln
195 200 205
Glu Arg Leu Lys Ala Phe Gly Lys Lys Asp Asn Ala Thr Pro Val Asn
210 215 220
His Val His Val Ile Gly Ala Gly Val Met Gly Gly Gly Ile Ala Ala
225 230 235 240
Trp Cys Ala Leu Gln Gly Ile Lys Thr Thr Leu Gln Asp Thr Asp Ala
245 250 255
Gln Arg Ile Ala Gly Ala Phe Lys Asn Ala Val Ser Ile Tyr Ala Arg
260 265 270
Lys Asp Arg Tyr Thr Ala Gln Ala Ala Arg Asp Arg Leu Ile Pro Asp
275 280 285
Leu Ala Gly His Gly Ile Ala Thr Ala Asp Leu Val Ile Glu Ala Ile
290 295 300
Ser Glu Asn Pro Gln Ala Lys Gln Ser Leu Tyr Gln Gln Ile Glu Pro
305 310 315 320
Lys Met Lys Glu Gly Ala Ile Leu Ala Thr Asn Thr Ser Ser Leu Ser
325 330 335
Ile Ala Gln Leu Arg Ser Val Leu Val His Pro Glu Arg Phe Val Gly
340 345 350
Ile His Phe Phe Asn Pro Val Ser Arg Met Pro Leu Val Glu Val Val
355 360 365
His Ala Asp Gly Ile Ala Gln Glu Thr Leu Asp Thr Ala Ala Ala Phe
370 375 380
Val Gly Lys Ile Gly Lys Leu Pro Leu Pro Val Gln Asp Thr Pro Gly
385 390 395 400
Phe Leu Val Asn Ala Val Leu Ala Pro Tyr Met Leu Gln Ala Met Arg
405 410 415
Cys Ile Asp Glu Gly Met Asp Pro Glu Val Ile Asp Thr Ala Met Leu
420 425 430
Glu Phe Gly Met Pro Met Gly Pro Ile Thr Leu Ala Asp Thr Val Gly
435 440 445
Leu Asp Ile Ala Met Ala Ala Gly Lys Gln Leu Ser Glu Gly Gln Glu
450 455 460
Pro Pro Arg Cys Leu Gln Glu Lys Ile Ala Gln Gly Lys Leu Gly Val
465 470 475 480
Lys Ser Gly Glu Gly Phe Tyr Val Trp Lys Asp Arg Lys His Asp Gln
485 490 495
Arg Ser Ser Lys Ala Ile Pro Gln Gly Leu Ala Gln Arg Leu Ile Lys
500 505 510
Pro Leu Ile Glu Gln Thr Glu Lys Gln Leu Ala Asn Asn Ile Val Gln
515 520 525
Asp Ala Asp Leu Ala Asp Ala Gly Val Ile Phe Gly Thr Gly Phe Ala
530 535 540
Pro Phe Thr Gly Gly Pro Ile His Tyr Lys Gln Ser Lys Gly Gly Leu
545 550 555 560
<210> 62
<211> 237
<212> PRT
<213> Oligotropha carboxidovorans
<400> 62
Met Ser Leu Ser Pro Leu Ala Asn Gly Val Arg Val Leu Thr Leu Asp
1 5 10 15
Arg Pro Ser Lys Ala Asn Ala Leu Asn Ala Glu Val Val Asp Gln Leu
20 25 30
Leu Ala Cys Val Ala Gln Ala Glu Ala Glu Asp Cys Arg Val Leu Ile
35 40 45
Leu Ala Ala Asn Gly Lys Ala Phe Cys Gly Gly Phe Asp Phe Gly Gly
50 55 60
Tyr Glu Ser Met Ser Ala Gly Asp Leu Leu Leu Arg Phe Val Arg Ile
65 70 75 80
Glu Glu Leu Leu Gln Arg Met Arg Gln Ser Ser Phe Val Ser Ile Ala
85 90 95
Leu Val His Gly Ala Ala Met Gly Ala Gly Ala Asp Ile Val Ala Ser
100 105 110
Cys Thr Tyr Arg Ile Gly Thr Asp Ala Ser Arg Phe Arg Phe Pro Gly
115 120 125
Phe Arg Phe Gly Val Ala Leu Gly Thr Arg His Leu Ala Gln Leu Val
130 135 140
Gly Pro Gln Arg Ala Arg Asp Ile Leu Leu Thr Asn Ala Thr Ile Asp
145 150 155 160
Ala Leu Thr Ala Val Asp Ile Gly Leu Leu Thr His Leu Val Asp Ala
165 170 175
Gly Ser Met Arg Gln Lys Ala Asp Glu Ile Ile Ala Gln Ile Gly Ser
180 185 190
Leu Asp Arg Val Ala Arg Asn Arg Ile Leu His Leu Thr Ser Ala Gln
195 200 205
Asn Asn Asp Gly Asp Met Ala Glu Leu Val Lys Ser Val Ser Ala Pro
210 215 220
Gly Leu His Glu Arg Ile Ala Gln Tyr Arg Ala Gly His
225 230 235
<210> 63
<211> 266
<212> PRT
<213> Riemerella anatipestifer
<400> 63
Met Tyr Lys Leu Ile Asp Val Asp Asn His Phe Glu Gly Lys Leu Gln
1 5 10 15
Ile Ala Tyr Ile Asn Gln Pro Glu Ser Phe Asn Ser Leu Asn Lys Val
20 25 30
Val Leu Glu Glu Leu Leu His Phe Ile Lys Ala Cys Asp Ala Asp Ser
35 40 45
Ser Val Arg Cys Ile Ala Ile Ser Gly Lys Gly Lys Ala Phe Cys Ser
50 55 60
Gly Gln Asn Leu Lys Glu Ala Leu Asp Tyr Lys Ala Glu Ala Asn Glu
65 70 75 80
Glu Arg Phe Ile Gln Arg Ile Val Ile Asp Tyr Tyr Asn Pro Leu Val
85 90 95
Lys Ala Ile Val Tyr Ala Lys Lys Pro Val Ile Ala Leu Val Asn Gly
100 105 110
Pro Ala Val Gly Ala Gly Ala Met Leu Ala Leu Ile Cys Asp Phe Ala
115 120 125
Val Ala Ser Glu Ser Ala Tyr Phe Ser Leu Ala Phe Ser Asn Ile Gly
130 135 140
Leu Val Pro Asp Thr Ala Gly Thr Tyr Tyr Leu Pro Lys Leu Leu Gly
145 150 155 160
Arg Ser Leu Ala Ser Tyr Leu Ala Phe Thr Gly Lys Lys Leu Ser Ala
165 170 175
Lys Glu Ser Leu Glu Arg Gly Leu Val Val Asp Val Phe Ser Asp Ala
180 185 190
Thr Phe Ser Glu Gln Ser Leu Gln Val Leu Glu His Ile Thr His Gln
195 200 205
Pro Thr Val Ala Leu Gly Leu Thr Lys Lys Ala Phe Asn Lys Ser Tyr
210 215 220
Gln Asn Ser Leu Ser Glu Gln Leu Asp Leu Glu Ser Ile Leu Gln Gln
225 230 235 240
Asp Ala Ala Glu Thr Trp Asp Phe Gln Glu Gly Ile Ala Ala Phe Leu
245 250 255
Ala Lys Arg Lys Pro Gln Tyr Lys Gly Lys
260 265
<210> 64
<211> 422
<212> PRT
<213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007
<400> 64
Met Ser Glu Thr Ile Asn Leu Asp Glu Met Ser Ala Lys Gln Leu Leu
1 5 10 15
Gly Tyr Tyr Gln Glu Lys Leu Asp Glu Glu Ala Arg Gln Ala Lys Arg
20 25 30
Glu Gly Lys Leu Val Cys Trp Ser Ala Ser Val Ala Pro Pro Glu Phe
35 40 45
Cys Val Ala Met Asp Ile Ala Met Val Tyr Pro Glu Thr His Ala Ala
50 55 60
Gly Ile Gly Ala Arg Lys Gly Ser Leu Asp Leu Leu Glu Val Ala Asp
65 70 75 80
Glu Lys Gly Tyr Ser Leu Asp Ile Cys Ser Tyr Ala Arg Val Asn Leu
85 90 95
Gly Tyr Met Glu Leu Leu Lys Gln Gln Ala Leu Thr Gly Glu Thr Pro
100 105 110
Glu Lys Leu Ala Asn Ser Pro Ala Ala Lys Val Pro Leu Pro Asp Leu
115 120 125
Val Ile Thr Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu
130 135 140
Asn Leu Ala Lys Glu Leu Asn Ile Pro Cys Ile Val Ile Asp Val Pro
145 150 155 160
Phe Asn His Thr Met Pro Ile Thr Lys His Ser Lys Glu Tyr Ile Ala
165 170 175
Asp Gln Phe Lys Tyr Ala Ile Gln Gln Leu Glu Glu Ile Thr Gly Lys
180 185 190
Lys Phe Asp Tyr Asp Lys Phe Leu Glu Val Gln Glu Gln Thr Gln Arg
195 200 205
Ser Val Tyr Gln Trp Asn Arg Leu Ala Ala Leu Ala His Tyr Lys Pro
210 215 220
Ser Pro Leu Asn Gly Phe Asp Leu Phe Asn Phe Met Ala Leu Ile Val
225 230 235 240
Cys Ala Arg Ser Arg Asp Tyr Ala Glu Ile Thr Phe Lys Lys Phe Ala
245 250 255
Asp Glu Leu Glu Glu Asn Leu Lys Asn Glu Val Tyr Ala Phe Lys Gly
260 265 270
Ala Glu Lys Asn Arg Val Thr Trp Glu Gly Ile Ala Val Trp Pro Tyr
275 280 285
Leu Gly His Thr Phe Lys Ser Leu Lys Gly Met Gly Ser Ile Met Thr
290 295 300
Gly Ser Ala Tyr Pro Gly Ile Trp Asn Leu Thr Tyr Thr Pro Gly Asp
305 310 315 320
Met Glu Ser Met Ala Glu Ala Tyr Thr Arg Val Tyr Ile Asn Thr Cys
325 330 335
Leu Gln Asn Lys Ala Asp Val Leu Ser Lys Ile Val Thr Asp Gly Lys
340 345 350
Cys Asp Gly Ile Leu Tyr His Leu Asn Arg Ser Cys Lys Leu Met Ser
355 360 365
Phe Leu Asn Val Glu Thr Ala Glu Leu Val Glu Lys Ala Thr Gly Val
370 375 380
Pro Tyr Val Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ala
385 390 395 400
Pro Ala Gln Phe Asp Thr Arg Val Gln Ala Leu Asn Glu Met Met Glu
405 410 415
Val Asn Asn Glu Thr Lys
420
<210> 65
<211> 277
<212> PRT
<213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007
<400> 65
Met Gln Asp Asp Arg Ser Phe Lys Lys Gly Lys Arg Arg Gly Met Tyr
1 5 10 15
Thr Val Gly Val Asp Ile Gly Ser Ser Ser Ser Lys Val Val Ile Leu
20 25 30
Lys Asp Gly Thr Glu Ile Val Ser Gln Ser Ala Ile Gln Ser Gly Ile
35 40 45
Gly Ser Asn Arg Ala Ile Val Ala Leu Glu Asp Asn Leu Lys Lys Ala
50 55 60
Asn Leu Thr Lys Glu Asp Ile Gly Phe Thr Val Val Thr Gly Tyr Gly
65 70 75 80
Arg Phe Thr Phe Glu Gly Ala Asp Lys Gln Ile Ser Glu Ile Ser Cys
85 90 95
His Ala Arg Gly Ile His Phe Leu Leu Pro Asn Val Arg Thr Ile Ile
100 105 110
Asp Ile Gly Gly Gln Asp Ala Lys Ala Ile Ser Leu Asp Glu Lys Gly
115 120 125
His Val Arg Gln Phe Phe Met Asn Asp Lys Cys Ala Ala Gly Thr Gly
130 135 140
Arg Phe Leu Thr Val Met Ala Arg Val Leu Glu Ile Ser Leu Asp Glu
145 150 155 160
Met Gly Thr Tyr Asp Ala Leu Ser Lys Asn Pro Cys Asn Ile Ser Ser
165 170 175
Thr Cys Ala Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu Ala Lys
180 185 190
Gly Asn Thr Lys Glu Asp Val Ile Ala Gly Val His Asn Ser Val Ala
195 200 205
His Lys Ile Leu Gly Leu Val Tyr Arg Thr Ser Met Glu Glu Lys Phe
210 215 220
Ala Ile Cys Gly Gly Val Ala Gln Asn Thr Gly Ala Leu Arg Ala Ile
225 230 235 240
Arg Glu Ala Leu Lys Lys Glu Val Ile Val Ala Pro Asn Pro Gln Leu
245 250 255
Thr Gly Ala Leu Gly Ala Ala Ile Phe Ala Tyr Asp Glu Leu Lys Lys
260 265 270
Leu Arg Lys Gly Glu
275
<210> 66
<211> 374
<212> PRT
<213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007
<400> 66
Met Lys Gly Arg Leu Glu Glu Leu Ile His Ile Phe Glu Asp Val Ala
1 5 10 15
Asn Asn Pro Lys Lys Met Val Ala Glu Tyr Lys Lys Glu Val Gly Lys
20 25 30
Glu Val Ile Gly Val Met Pro Val Tyr Ala Pro Glu Glu Ile Ile His
35 40 45
Ala Ala Gly Cys Leu Pro Ile Gly Leu Trp Gly Gly Lys Lys Glu Val
50 55 60
Ser Lys Ala Arg Ala Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln
65 70 75 80
Thr Val Met Glu Leu Gln Ile Gly Gly Thr Tyr Asp Ile Leu Asp Ala
85 90 95
Val Leu Phe Ser Val Pro Cys Asp Thr Leu Lys Cys Leu Ser Gln Lys
100 105 110
Trp Lys Gly Lys Ser Pro Val Ile Val Phe Thr His Pro Gln Asn Arg
115 120 125
Val Ile Glu Gly Ala Asn Ala Tyr Leu Val Lys Glu Tyr Gln Ala Val
130 135 140
Lys Glu Lys Leu Glu Gly Ile Leu Gly Arg Thr Ile Pro Met Glu Ala
145 150 155 160
Ile Glu Glu Ser Val Lys Val Tyr Asn Glu Asn Arg Arg Val Met Arg
165 170 175
Glu Phe Val Glu Val Ala Ala Gln Tyr Pro Gln Ile Ile Asp Pro Ile
180 185 190
Val Arg His Asn Val Met Lys Ser Arg Trp Phe Leu Arg Lys Glu Lys
195 200 205
His Thr Glu Tyr Val Lys Glu Leu Ile Ala Glu Leu Lys Lys Glu Thr
210 215 220
Ile Val Pro Trp Asp Gly Lys Lys Val Ile Leu Thr Gly Ile Met Thr
225 230 235 240
Glu Pro Val Glu Leu Leu Gln Ile Phe Lys Asp Glu Lys Leu Ala Ile
245 250 255
Val Ala Asp Asp Leu Ala His Glu Ser Arg Gln Phe Arg Gly Asp Val
260 265 270
Pro Glu Glu Gly Gly Asp Val Leu Tyr Arg Met Ala Lys Trp Trp Gln
275 280 285
Asn Leu Glu Gly Cys Ser Leu Ala Thr Asp Thr Asn Lys Gly Arg Gly
290 295 300
Gln Met Leu Met Asp Met Cys Lys Asp Thr Lys Ala Asp Ala Val Ile
305 310 315 320
Val Cys Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Val
325 330 335
Tyr Tyr Arg Glu Phe Thr Glu Ser Gly Ile Lys Asn Ile Thr Val Glu
340 345 350
Val Asp Leu Glu Val Ser Ser Phe Glu Gln Ile Arg Thr Arg Ile Gln
355 360 365
Thr Phe Lys Asp Ile Leu
370
<210> 67
<211> 422
<212> PRT
<213> Desulfosporosinus youngiae DSM 17734
<400> 67
Met Thr Asp Thr Thr Thr Met Ser Ala Lys Glu Leu Leu Gly Phe Tyr
1 5 10 15
Gln Glu Glu Leu Tyr Glu Glu Ala Arg Gln Ala Lys Lys Glu Gly Lys
20 25 30
Leu Val Cys Trp Ser Ala Ser Val Ala Pro Ser Glu Phe Cys Val Ala
35 40 45
Met Asp Val Ala Met Ile Tyr Pro Glu Thr His Ala Ala Gly Ile Gly
50 55 60
Ala Arg Lys Gly Ala Leu Asp Val Leu Glu Val Ala Asp Glu Lys Gly
65 70 75 80
Tyr Asn Leu Asp Thr Cys Ser Tyr Ala Arg Val Asn Met Gly Tyr Met
85 90 95
Glu Leu Leu Lys Gln Glu Ala Leu Thr Gly Ile Thr Pro Glu Lys Leu
100 105 110
Glu Lys Ser Pro Ala Ala Arg Ile Pro Leu Pro Asp Phe Val Ile Thr
115 120 125
Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu Asn Leu Ala
130 135 140
Val Glu Leu Asn Ile Pro Cys Ile Ile Ile Asp Val Pro Phe Asn His
145 150 155 160
Thr Met Pro Ile Pro Gln Tyr Ala Lys Asp Tyr Ile Ala Glu Gln Phe
165 170 175
Lys Glu Ala Ile Thr Gln Leu Glu Glu Ile Cys Gly Arg Lys Phe Asp
180 185 190
Tyr Asp Lys Phe Leu Lys Val Gln Glu Gln Thr Gln Arg Ser Val Ala
195 200 205
Gln Trp Asn Arg Ile Ala Ala Leu Ser Gly His Lys Pro Ser Pro Leu
210 215 220
Asn Gly Phe Asp Leu Phe Asn Tyr Met Ala Leu Ile Val Cys Ala Arg
225 230 235 240
Ser Arg Asp Tyr Ala Glu Ile Thr Phe Lys Lys Phe Ala Asp Glu Leu
245 250 255
Glu Glu Asn Leu Lys Asn Gly Ile Tyr Ala Phe Lys Gly Asn Glu Gln
260 265 270
Lys Arg Val Thr Trp Glu Gly Ile Ala Val Trp Pro His Leu Gly His
275 280 285
Thr Phe Lys Gly Leu Lys Asn Leu Gly Asn Ile Met Thr Gly Ser Ala
290 295 300
Tyr Pro Gly Leu Trp Asn Leu Thr Tyr Thr Pro Gly Asp Met Ser Ser
305 310 315 320
Met Ala Glu Ala Tyr Thr Arg Ile Tyr Ile Asn Thr Cys Leu Asp Asn
325 330 335
Lys Val Lys Val Leu Ser Asp Val Ile Ser Gly Gly Lys Cys Asp Gly
340 345 350
Val Ile Tyr His Gln Asn Arg Ser Cys Lys Leu Met Ser Leu Leu Asn
355 360 365
Val Glu Thr Ala Asp Ile Leu Gln Lys Gln Asn His Leu Pro Tyr Val
370 375 380
Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ala Pro Ala Gln
385 390 395 400
Phe Asp Thr Arg Ile Gln Ala Leu Asp Glu Met Met Lys Gln Asn Lys
405 410 415
Glu Gly Val Ser Asn Glu
420
<210> 68
<211> 372
<212> PRT
<213> Desulfosporosinus youngiae DSM 17734
<400> 68
Met Ser Arg Ile Glu Thr Ile Ile Ser Glu Leu Thr Ser Ile Ala Asn
1 5 10 15
Asn Pro Arg Gln Ala Met Glu Asp Tyr Lys Lys Glu Thr Gly Lys Gly
20 25 30
Ser Val Gly Val Met Pro Tyr Tyr Ala Pro Glu Glu Ile Ile His Ala
35 40 45
Ala Gly Tyr Leu Pro Val Gly Ile Trp Gly Gly Gln Lys Ser Ile Ser
50 55 60
Lys Ala Arg Ala Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln Ser
65 70 75 80
Val Val Glu Met Gln Leu Glu Gly Val Tyr Asp Asp Leu Glu Ala Val
85 90 95
Leu Phe Pro Val Pro Cys Asp Thr Leu Lys Cys Leu Ser Gln Lys Trp
100 105 110
Lys Gly Thr Ser Pro Val Ile Val Leu Thr His Pro Gln Asn Arg Lys
115 120 125
Leu Glu Ala Ala Asn Lys Phe Leu Ala Glu Glu Tyr Arg Leu Val Arg
130 135 140
Glu Lys Leu Glu Lys Ile Leu Asn Val Lys Ile Thr Asp Glu Ala Leu
145 150 155 160
Asn Gln Ser Ile Glu Ile Tyr Asn Glu Asn Arg Lys Val Met Arg Glu
165 170 175
Phe Thr Glu Ile Ala Ala Asn Tyr Pro Asn Ile Ile Asp Pro Val Lys
180 185 190
Arg His Ala Leu Ile Lys Ala Arg Phe Phe Met Glu Lys Ala Lys His
195 200 205
Thr Ala Leu Val Lys Glu Leu Asn Ala Glu Leu Lys Ala Leu Pro Val
210 215 220
Glu Ala Phe Thr Gly Lys Lys Val Val Leu Thr Gly Ile Met Ala Glu
225 230 235 240
Pro Asn Glu Val Leu Asp Ile Leu Gln Asp Asn Gly Phe Ala Val Val
245 250 255
Ala Asp Asp Leu Ala Gln Glu Ser Arg Leu Phe Arg Asn Asp Val Pro
260 265 270
Ser Gly Thr Asp Pro Leu Tyr Arg Leu Ala Lys Trp Trp Gln Glu Phe
275 280 285
Asp Gly Cys Ser Leu Ala Val Asp Ala Lys Lys Pro Arg Gly Pro Met
290 295 300
Leu Met Asp Met Val Lys Ala Ser Lys Ala Asp Ala Val Val Val Cys
305 310 315 320
Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Ile Tyr Tyr
325 330 335
Arg Gln Phe Glu Glu Ala Gly Ile Lys Ser Leu Phe Ile Glu Ile Asp
340 345 350
Leu Glu Pro Thr Ser Phe Glu Gln Thr Lys Thr Arg Val Gln Ser Phe
355 360 365
Arg Glu Met Leu
370
<210> 69
<211> 272
<212> PRT
<213> Desulfosporosinus youngiae DSM 17734
<400> 69
Met Phe Thr Met Gly Ile Asp Ile Gly Ser Ser Ser Ser Lys Val Val
1 5 10 15
Ile Leu Glu Asp Gly Val Asn Ile Ile Ala Gly Glu Val Ile Gln Ile
20 25 30
Gly Thr Gly Ser Thr Gly Pro Lys Arg Val Leu Asp Glu Ala Leu Ala
35 40 45
Lys Ala Gly Leu Thr Leu Gln Asp Met Ala Lys Ile Ile Ala Thr Gly
50 55 60
Tyr Gly Arg Ser Ser Val Glu Glu Ala His Lys Gln Ile Ser Glu Ile
65 70 75 80
Ser Cys Gln Ala Lys Gly Val Phe Phe Leu Val Pro Ser Ala Lys Leu
85 90 95
Ile Ile Asp Ile Gly Gly Gln Asp Val Lys Ala Ile Lys Leu Asp Ser
100 105 110
Lys Gly Cys Val Lys Gln Phe Phe Met Asn Asp Lys Cys Ala Ala Gly
115 120 125
Thr Gly Arg Phe Leu Asp Val Met Ser Arg Val Leu Glu Val Asn Leu
130 135 140
Asp Glu Met Ala Glu Tyr Asp Ala Arg Ala Thr Glu Pro Ala Thr Val
145 150 155 160
Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu
165 170 175
Ala Asn Gly Val Ala Lys Glu Asn Ile Ile Ala Gly Val His Gln Ser
180 185 190
Val Ala Ser Lys Ala Cys Gly Leu Ala Tyr Arg Cys Gly Val Glu Glu
195 200 205
Asp Ile Val Met Cys Gly Gly Val Ala Lys Asp Leu Gly Val Val Arg
210 215 220
Ala Ile Ser Lys Glu Leu Lys Lys Pro Val Ile Val Ala Pro Asn Pro
225 230 235 240
Gln Ile Thr Ala Ala Leu Gly Ala Ala Ile Phe Ala Phe Glu Glu Val
245 250 255
Met Glu Thr Val Met Val Ala Phe Glu Glu Val Arg Gly Ala Asn Lys
260 265 270
<210> 70
<211> 422
<212> PRT
<213> Peptoniphilus indolicus ATCC 29427
<400> 70
Met Asn Thr Ile Asp Ile Ser Asn Met Lys Ala Lys Glu Met Leu Gly
1 5 10 15
Tyr Phe Gln Asn Lys Leu Asp Glu Glu Ala Arg Glu Ala Lys Lys Asn
20 25 30
Gly Lys Leu Val Cys Trp Ser Ala Ser Val Ala Pro Ser Glu Phe Cys
35 40 45
Val Thr Met Asp Ile Ala Leu Val Tyr Pro Glu Thr His Ala Ala Gly
50 55 60
Ile Gly Ala Arg Lys Gly Ser Leu Ala Met Leu Asp Val Ala Asp Arg
65 70 75 80
Lys Gly Tyr Asn Thr Asp Ile Cys Ser Tyr Ala Arg Val Asn Leu Gly
85 90 95
Tyr Met Glu Leu Leu Lys Glu Tyr Ala Lys Thr Gly Val Lys Pro Lys
100 105 110
Glu Leu Glu Glu Ser Pro Ala Ala Asp Val Pro Leu Pro Asp Leu Val
115 120 125
Ile Thr Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu Asn
130 135 140
Leu Ala Ala Glu Leu Asn Ile Pro Cys Ile Val Ile Asp Val Pro Phe
145 150 155 160
Asn His Thr Met Pro Ile Pro Lys Tyr Ser Lys Glu Tyr Ile Ala Asp
165 170 175
Gln Phe Lys Glu Ala Ile Arg Gln Leu Glu Glu Ile Thr Gly Lys Asp
180 185 190
Phe Asp Tyr Asp Lys Phe Leu Glu Val Gln Glu Gln Thr Gln Arg Ser
195 200 205
Val Ala Gln Trp Asn Arg Leu Ala Ala Leu Ser Lys Tyr Glu Pro Ser
210 215 220
Pro Leu Asn Gly Phe Asp Leu Phe Asn Tyr Met Ala Leu Ile Val Cys
225 230 235 240
Ala Arg Ser Lys Asn Tyr Ala Glu Leu Thr Phe Lys Lys Phe Ala Asp
245 250 255
Glu Leu Glu Glu Asn Met Gln Asn Gly Val Tyr Pro Tyr Lys Ala Gly
260 265 270
Glu Gln Ser Arg Ile Thr Trp Glu Gly Ile Ala Ile Trp Pro Tyr Leu
275 280 285
Gly His Thr Phe Lys Thr Leu Lys Gly Tyr Gly Ser Ile Met Thr Gly
290 295 300
Ser Ala Tyr Pro Gly Leu Trp Asn Leu Glu Tyr Thr Pro Gly Asp Met
305 310 315 320
Leu Ser Met Ala Glu Ala Tyr Thr Arg Ile Tyr Ile Asn Thr Cys Leu
325 330 335
Asp Asn Lys Val Asp Val Leu Arg Lys Ile Ile Lys Asn Gly Lys Cys
340 345 350
Asp Gly Val Ala Tyr His Leu Asn Arg Ser Cys Lys Leu Met Ser Leu
355 360 365
Leu Asn Val Glu Thr Ala Glu Ile Leu Asn Lys Glu Asn Asn Leu Pro
370 375 380
Tyr Val Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ser Glu
385 390 395 400
Ala Gln Tyr Asp Asn Arg Ile Gln Thr Leu Thr Glu Met Met Ser Ala
405 410 415
Asn Lys Lys Met Arg Gly
420
<210> 71
<211> 263
<212> PRT
<213> Peptoniphilus indolicus ATCC 29427
<400> 71
Met Tyr Thr Met Gly Val Asp Ile Gly Ser Thr Ser Ser Lys Ile Ile
1 5 10 15
Ile Leu Glu Asp Gly Ile Lys Ile Ile Gly Asn Ile Val Val Gln Ser
20 25 30
Gly Thr Gly Thr Ser Gly Pro Thr Ile Ala Thr Ala Lys Ala Lys Ser
35 40 45
Phe Leu Ser Asn Asn Asn Leu Thr Leu Asp Asp Ile Ser Lys Ile Val
50 55 60
Val Thr Gly Tyr Gly Arg Phe Ser Phe Asp Ile Ala Asp Lys Gln Ile
65 70 75 80
Ser Glu Ile Thr Cys His Thr Lys Gly Ile Asn Phe Leu Val Pro Glu
85 90 95
Ala Arg Thr Ile Leu Asp Ile Gly Gly Gln Asp Thr Lys Ala Ile Ser
100 105 110
Val Asn Asp Lys Gly Gln Val Leu Gln Phe Phe Met Asn Asp Lys Cys
115 120 125
Ala Ala Gly Thr Gly Arg Phe Leu Glu Val Met Ala Lys Ile Leu Glu
130 135 140
Ile Pro Leu Glu Lys Met Gly Glu Tyr Asp Arg Leu Ser Thr Asn Pro
145 150 155 160
Val Ala Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile
165 170 175
Ser Gln Leu Ser Lys Gly Ile Ser Lys Glu Asn Ile Leu Ala Gly Val
180 185 190
His Asn Ser Thr Ala Asn Lys Val Cys Gly Leu Leu Tyr Arg Thr Gly
195 200 205
Ile Lys Glu Lys Ile Val Leu Cys Gly Gly Val Ala Gln Asn Gln Gly
210 215 220
Val Val Arg Ala Leu Gln Glu Glu Leu Lys Lys Glu Ile Thr Ile Ala
225 230 235 240
Pro His Pro Gln Met Thr Gly Ala Ile Gly Ala Ala Leu Phe Ala Tyr
245 250 255
Glu Glu Ala Asn Lys Asn Leu
260
<210> 72
<211> 372
<212> PRT
<213> Peptoniphilus indolicus ATCC 29427
<400> 72
Met Asn Lys Ile Asn Glu Ile Ile Asn Leu Leu Asp Glu Val Ser Lys
1 5 10 15
Asp Pro Lys Leu Thr Val Lys Lys Tyr Lys Glu Lys Thr Gly Lys Gly
20 25 30
Val Val Gly Val Met Pro Leu Tyr Ala Pro Glu Glu Ile Ile His Ala
35 40 45
Ala Gly Phe Leu Pro Met Gly Leu Trp Gly Ala Gln Lys Glu Val Ser
50 55 60
Lys Ala Arg Ile Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln Thr
65 70 75 80
Asn Met Glu Leu Gln Ile Glu Gly Ala Tyr Asp Asp Leu Asp Ala Val
85 90 95
Val Phe Ser Val Pro Cys Asp Thr Leu Lys Cys Met Ser Gln Lys Trp
100 105 110
Lys Gly Lys Ser Pro Val Ile Val Phe Thr His Pro Gln Asn Arg Lys
115 120 125
Leu Glu Ser Ala Asn Lys Phe Leu Val Thr Glu Tyr Glu Ile Leu Lys
130 135 140
Asp Lys Leu Glu Lys Ile Leu Asn Val Lys Ile Ser Asp Glu Ser Ile
145 150 155 160
Thr Asn Ser Ile Glu Ile Tyr Asn Glu Asn Arg Lys Val Met Arg Glu
165 170 175
Phe Ser Asp Leu Ala Gly Gln Tyr Pro Asn Ile Ile Asp Pro Ile Gln
180 185 190
Arg His Ile Val Phe Lys Ser Arg Trp Phe Met Glu Lys Ser Glu His
195 200 205
Thr Lys Leu Val Lys Glu Leu Ile Ser Glu Ile Lys Lys Leu Pro Ile
210 215 220
Glu Glu Trp Asp Gly Tyr Lys Val Ile Ala Thr Gly Ile Met Ile Glu
225 230 235 240
Pro Glu Glu Ile Leu Gln Ile Phe Lys Asp Lys Lys Ile Ala Ile Val
245 250 255
Ala Asp Asp Leu Ala Gln Glu Ser Arg Gln Phe Arg His Asp Val Pro
260 265 270
Glu Gly Asp Gln Pro Leu Leu Arg Leu Ala Lys Trp Trp Gln Asn Leu
275 280 285
Glu Gly Cys Ala Leu Ala Thr Asp Thr Lys Lys Leu Arg Gly Gln Met
290 295 300
Leu Ile Asp Met Ala Lys Lys Tyr Asn Ala Asp Ala Val Leu Ile Cys
305 310 315 320
Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Val Tyr Tyr
325 330 335
Arg Glu Phe Gln Glu Ala Gly Ile Lys Asn Leu Leu Ile Glu Ile Asp
340 345 350
Leu Glu Met Thr Ala Phe Glu Gln Thr Asn Thr Arg Leu Gln Thr Leu
355 360 365
Val Glu Thr Leu
370
<210> 73
<211> 422
<212> PRT
<213> Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10)
<400> 73
Met Thr Asp Thr Thr Ala Met Ser Ala Lys Glu Leu Leu Gly Phe Tyr
1 5 10 15
Gln Glu Glu Leu Tyr Glu Glu Ala Arg Arg Ala Lys Lys Glu Gly Lys
20 25 30
Leu Val Cys Trp Ser Ala Ser Val Ala Pro Ser Glu Phe Cys Val Ala
35 40 45
Met Asp Val Ala Met Ile Tyr Pro Glu Thr His Ala Ala Gly Ile Gly
50 55 60
Ala Arg Lys Gly Ala Leu Asp Val Leu Glu Val Ala Asp Glu Lys Gly
65 70 75 80
Tyr Asn Val Asp Thr Cys Ser Tyr Ala Arg Val Asn Leu Gly Tyr Met
85 90 95
Glu Leu Leu Lys Gln Glu Ala Leu Thr Gly Ile Thr Pro Glu Lys Leu
100 105 110
Glu Lys Ser Pro Ala Ala Arg Ile Pro Leu Pro Asp Phe Val Ile Thr
115 120 125
Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu Asn Leu Ala
130 135 140
Val Glu Leu Asn Ile Pro Cys Ile Ile Ile Asp Val Pro Phe Asn His
145 150 155 160
Thr Met Pro Ile Pro Gln Tyr Ala Lys Asp Tyr Ile Ala Glu Gln Phe
165 170 175
Lys Glu Ala Ile Thr Gln Leu Glu Glu Ile Cys Gly Lys Lys Phe Asp
180 185 190
Tyr Asp Lys Phe Leu Lys Val Gln Glu Gln Thr Gln Arg Ser Val Ala
195 200 205
Gln Trp Asn Arg Ile Ala Ala Leu Ser Ser His Lys Pro Ser Pro Leu
210 215 220
Asn Gly Phe Asp Leu Phe Asn Tyr Met Ala Leu Ile Val Cys Ala Arg
225 230 235 240
Ser Lys Asp Tyr Ala Glu Ile Thr Phe Lys Lys Phe Ala Asp Glu Leu
245 250 255
Glu Glu Asn Leu Asn Lys Gly Ile Phe Ala Phe Lys Gly Asn Glu Gln
260 265 270
Lys Arg Val Thr Trp Glu Gly Ile Ala Val Trp Pro His Leu Gly His
275 280 285
Thr Phe Lys Gly Leu Lys Asn Leu Gly Asn Ile Met Thr Gly Ser Ala
290 295 300
Tyr Pro Gly Leu Trp Asn Val Ser Tyr Thr Pro Gly Asp Met Ser Ser
305 310 315 320
Met Ala Glu Ala Tyr Thr Arg Ile Tyr Ile Asn Thr Cys Leu Asp Asn
325 330 335
Lys Val Lys Val Leu Ser Asp Val Ile Ser Gly Gly Lys Cys Asp Gly
340 345 350
Val Ile Tyr His Gln Asn Arg Ser Cys Lys Leu Met Ser Phe Leu Asn
355 360 365
Val Glu Thr Ala Asp Ile Leu Gln Lys Glu Asn Gly Leu Pro Tyr Val
370 375 380
Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ser Pro Ala Gln
385 390 395 400
Phe Asp Thr Arg Ile Gln Ala Leu Asp Glu Met Met Lys Gln Asn Lys
405 410 415
Glu Gly Val Ser Asn Glu
420
<210> 74
<211> 372
<212> PRT
<213> Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10)
<400> 74
Met Ser Arg Ile Glu Thr Ile Ile Ser Glu Leu Ser Ser Ile Ser Asn
1 5 10 15
Asn Pro Arg Lys Ala Met Glu Asp Tyr Lys Lys Glu Thr Gly Lys Gly
20 25 30
Ser Val Gly Val Met Pro Tyr Tyr Ala Pro Glu Glu Ile Ile His Ala
35 40 45
Ala Gly Phe Leu Pro Val Gly Ile Trp Gly Gly Gln Lys Ser Ile Ser
50 55 60
Lys Ala Arg Ala Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln Ser
65 70 75 80
Val Met Glu Met Gln Leu Glu Gly Val Tyr Asp Asp Leu Glu Ala Val
85 90 95
Leu Phe Pro Val Pro Cys Asp Thr Leu Lys Cys Leu Ser Gln Lys Trp
100 105 110
Lys Gly Thr Ser Pro Val Ile Val Phe Thr His Pro Gln Asn Arg Lys
115 120 125
Leu Glu Ala Ala Asn Lys Phe Leu Ala Glu Glu Tyr Arg Leu Val Arg
130 135 140
Glu Lys Leu Glu Thr Ile Leu Asn Val Lys Ile Thr Asp Glu Ala Leu
145 150 155 160
Asn Gln Ser Ile Glu Thr Tyr Asn Glu Asn Arg Lys Val Met Arg Glu
165 170 175
Phe Thr Asp Leu Ala Ala Asn Tyr Pro Gln Ile Ile Asp Pro Arg Ile
180 185 190
Arg His Ala Ile Ile Lys Ala Arg Phe Phe Met Glu Lys Ser Lys His
195 200 205
Thr Ala Met Val Lys Glu Leu Asn Ser Glu Leu Lys Ser Leu Pro Val
210 215 220
Glu Ala Phe Thr Gly Lys Lys Val Val Leu Thr Gly Ile Met Ala Glu
225 230 235 240
Pro Asn Glu Val Leu Asp Ile Leu Lys Asp Asn Gly Phe Ala Val Val
245 250 255
Ala Asp Asp Leu Ala Gln Glu Ser Arg Leu Phe Arg Asn Asp Val Pro
260 265 270
Ser Gly Thr Asp Pro Leu Tyr Arg Leu Ala Lys Trp Trp Gln Glu Phe
275 280 285
Asp Gly Cys Ser Leu Ala Thr Asp Ala Lys Lys Ser Arg Gly Pro Met
290 295 300
Leu Met Glu Met Val Lys Gly Ser Lys Ala Asp Ala Val Val Val Cys
305 310 315 320
Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Ile Tyr Tyr
325 330 335
Arg Gln Phe Glu Glu Ala Gly Ile Lys Ser Leu Phe Ile Glu Ile Asp
340 345 350
Leu Glu Thr Thr Ser Phe Glu Gln Thr Lys Thr Arg Val Gln Ser Phe
355 360 365
Ser Glu Met Leu
370
<210> 75
<211> 261
<212> PRT
<213> Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10)
<400> 75
Met Phe Thr Met Gly Ile Asp Ile Gly Ser Ser Ser Ser Lys Val Val
1 5 10 15
Ile Leu Glu Asp Gly Val Asn Ile Ile Ala Gly Glu Val Ile Gln Ile
20 25 30
Gly Thr Gly Ser Thr Gly Pro Lys Arg Val Leu Asn Glu Ala Leu Ser
35 40 45
Lys Ala Gly Leu Lys Leu Glu Asp Met Ala Lys Ile Ile Ala Thr Gly
50 55 60
Tyr Gly Arg Ser Ser Val Glu Glu Ala His Lys Gln Ile Ser Glu Ile
65 70 75 80
Ser Cys Gln Ala Lys Gly Val Phe Phe Leu Val Pro Ser Ala Lys Leu
85 90 95
Ile Ile Asp Ile Gly Gly Gln Asp Val Lys Ala Ile Arg Leu Asp Ser
100 105 110
Lys Gly Gly Val Lys Gln Phe Phe Met Asn Asp Lys Cys Ala Ala Gly
115 120 125
Thr Gly Arg Phe Leu Asp Val Met Ser Arg Val Leu Glu Val Asn Leu
130 135 140
Asp Glu Met Ala Glu Tyr Asp Ala Arg Ala Thr Glu Pro Ala Thr Val
145 150 155 160
Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu
165 170 175
Ser Asn Gly Val Ala Lys Glu Asn Ile Ile Ala Gly Val His Gln Ser
180 185 190
Val Ala Ser Lys Ala Cys Gly Leu Ala Tyr Arg Cys Gly Val Glu Glu
195 200 205
Asp Ile Val Met Cys Gly Gly Val Ala Lys Asp Leu Gly Val Val Arg
210 215 220
Ala Ile Ser Lys Glu Leu Lys Lys Pro Val Ile Val Ala Pro Asn Pro
225 230 235 240
Gln Ile Thr Ala Ala Leu Gly Ala Ala Ile Phe Ala Phe Glu Glu Val
245 250 255
Arg Gly Ala Asn Lys
260
<210> 76
<211> 477
<212> PRT
<213> Acidaminococcus fermentans
<400> 76
Met Pro Lys Thr Val Ser Pro Gly Val Gln Ala Leu Arg Asp Val Val
1 5 10 15
Glu Lys Val Tyr Arg Glu Leu Arg Glu Ala Lys Glu Arg Gly Glu Lys
20 25 30
Val Gly Trp Ser Ser Ser Lys Phe Pro Cys Glu Leu Ala Glu Ser Phe
35 40 45
Gly Leu His Val Gly Tyr Pro Glu Asn Gln Ala Ala Gly Ile Ala Ala
50 55 60
Asn Arg Asp Gly Glu Val Met Cys Gln Ala Ala Glu Asp Ile Gly Tyr
65 70 75 80
Asp Asn Asp Ile Cys Gly Tyr Ala Arg Ile Ser Leu Ala Tyr Ala Ala
85 90 95
Gly Phe Arg Gly Ala Asn Lys Met Asp Lys Asp Gly Asn Tyr Val Ile
100 105 110
Asn Pro His Ser Gly Lys Gln Met Lys Asp Ala Asn Gly Lys Lys Val
115 120 125
Phe Asp Ala Asp Gly Lys Pro Val Ile Asp Pro Lys Thr Leu Lys Pro
130 135 140
Phe Ala Thr Thr Asp Asn Ile Tyr Glu Ile Ala Ala Leu Pro Glu Gly
145 150 155 160
Glu Glu Lys Thr Arg Arg Gln Asn Ala Leu His Lys Tyr Arg Gln Met
165 170 175
Thr Met Pro Met Pro Asp Phe Val Leu Cys Cys Asn Asn Ile Cys Asn
180 185 190
Cys Met Thr Lys Trp Tyr Glu Asp Ile Ala Arg Arg His Asn Ile Pro
195 200 205
Leu Ile Met Ile Asp Val Pro Tyr Asn Glu Phe Asp His Val Asn Glu
210 215 220
Ala Asn Val Lys Tyr Ile Arg Ser Gln Leu Asp Thr Ala Ile Arg Gln
225 230 235 240
Met Glu Glu Ile Thr Gly Lys Lys Phe Asp Glu Asp Lys Phe Glu Gln
245 250 255
Cys Cys Gln Asn Ala Asn Arg Thr Ala Lys Ala Trp Leu Lys Val Cys
260 265 270
Asp Tyr Leu Gln Tyr Lys Pro Ala Pro Phe Asn Gly Phe Asp Leu Phe
275 280 285
Asn His Met Ala Asp Val Val Thr Ala Arg Gly Arg Val Glu Ala Ala
290 295 300
Glu Ala Phe Glu Leu Leu Ala Lys Glu Leu Glu Gln His Val Lys Glu
305 310 315 320
Gly Thr Thr Thr Ala Pro Phe Lys Glu Gln His Arg Ile Met Phe Glu
325 330 335
Gly Ile Pro Cys Trp Pro Lys Leu Pro Asn Leu Phe Lys Pro Leu Lys
340 345 350
Ala Asn Gly Leu Asn Ile Thr Gly Val Val Tyr Ala Pro Ala Phe Gly
355 360 365
Phe Val Tyr Asn Asn Leu Asp Glu Leu Val Lys Ala Tyr Cys Lys Ala
370 375 380
Pro Asn Ser Val Ser Ile Glu Gln Gly Val Ala Trp Arg Glu Gly Leu
385 390 395 400
Ile Arg Asp Asn Lys Val Asp Gly Val Leu Val His Tyr Asn Arg Ser
405 410 415
Cys Lys Pro Trp Ser Gly Tyr Met Pro Glu Met Gln Arg Arg Phe Thr
420 425 430
Lys Asp Met Gly Ile Pro Thr Ala Gly Phe Asp Gly Asp Gln Ala Asp
435 440 445
Pro Arg Asn Phe Asn Ala Ala Gln Tyr Glu Thr Arg Val Gln Gly Leu
450 455 460
Val Glu Ala Met Glu Ala Asn Asp Glu Lys Lys Gly Lys
465 470 475
<210> 77
<211> 379
<212> PRT
<213> Acidaminococcus fermentans
<400> 77
Met Ala Ile Ser Ala Leu Ile Glu Glu Phe Gln Lys Val Ser Ala Ser
1 5 10 15
Pro Lys Thr Met Leu Ala Lys Tyr Lys Ala Gln Gly Lys Lys Ala Ile
20 25 30
Gly Cys Leu Pro Tyr Tyr Val Pro Glu Glu Leu Val Tyr Ala Ala Gly
35 40 45
Met Val Pro Met Gly Val Trp Gly Cys Asn Gly Lys Gln Glu Val Arg
50 55 60
Ser Lys Glu Tyr Cys Ala Ser Phe Tyr Cys Thr Ile Ala Gln Gln Ser
65 70 75 80
Leu Glu Met Leu Leu Asp Gly Thr Leu Asp Gly Leu Asp Gly Ile Ile
85 90 95
Thr Pro Val Leu Cys Asp Thr Leu Arg Pro Met Ser Gln Asn Phe Lys
100 105 110
Val Ala Met Lys Asp Lys Met Pro Val Ile Phe Leu Ala His Pro Gln
115 120 125
Val Arg Gln Asn Ala Ala Gly Lys Gln Phe Thr Tyr Asp Ala Tyr Ser
130 135 140
Glu Val Lys Gly His Leu Glu Glu Ile Cys Gly His Glu Ile Thr Asn
145 150 155 160
Asp Ala Ile Leu Asp Ala Ile Lys Val Tyr Asn Lys Ser Arg Ala Ala
165 170 175
Arg Arg Glu Phe Cys Lys Leu Ala Asn Glu His Pro Asp Leu Ile Pro
180 185 190
Ala Ser Val Arg Ala Thr Val Leu Arg Ala Ala Tyr Phe Met Leu Lys
195 200 205
Asp Glu Tyr Thr Glu Lys Leu Glu Glu Leu Asn Lys Glu Leu Ala Ala
210 215 220
Ala Pro Ala Gly Lys Phe Asp Gly His Lys Val Val Val Ser Gly Ile
225 230 235 240
Ile Tyr Asn Met Pro Gly Ile Leu Lys Ala Met Asp Asp Asn Lys Leu
245 250 255
Ala Ile Ala Ala Asp Asp Cys Ala Tyr Glu Ser Arg Ser Phe Ala Val
260 265 270
Asp Ala Pro Glu Asp Leu Asp Asn Gly Leu Gln Ala Leu Ala Val Gln
275 280 285
Phe Ser Lys Gln Lys Asn Asp Val Leu Leu Tyr Asp Pro Glu Phe Ala
290 295 300
Lys Asn Thr Arg Ser Glu His Val Cys Asn Leu Val Lys Glu Ser Gly
305 310 315 320
Ala Glu Gly Leu Ile Val Phe Met Met Gln Phe Cys Asp Pro Glu Glu
325 330 335
Met Glu Tyr Pro Asp Leu Lys Lys Ala Leu Asp Ala His His Ile Pro
340 345 350
His Val Lys Ile Gly Val Asp Gln Met Thr Arg Asp Phe Gly Gln Ala
355 360 365
Gln Thr Ala Leu Glu Ala Phe Ala Glu Ser Leu
370 375
<210> 78
<211> 260
<212> PRT
<213> Acidaminococcus fermentans
<400> 78
Met Ser Ile Tyr Thr Leu Gly Ile Asp Val Gly Ser Thr Ala Ser Lys
1 5 10 15
Cys Ile Ile Leu Lys Asp Gly Lys Glu Ile Val Ala Lys Ser Leu Val
20 25 30
Ala Val Gly Thr Gly Thr Ser Gly Pro Ala Arg Ser Ile Ser Glu Val
35 40 45
Leu Glu Asn Ala His Met Lys Lys Glu Asp Met Ala Phe Thr Leu Ala
50 55 60
Thr Gly Tyr Gly Arg Asn Ser Leu Glu Gly Ile Ala Asp Lys Gln Met
65 70 75 80
Ser Glu Leu Ser Cys His Ala Met Gly Ala Ser Phe Ile Trp Pro Asn
85 90 95
Val His Thr Val Ile Asp Ile Gly Gly Gln Asp Val Lys Val Ile His
100 105 110
Val Glu Asn Gly Thr Met Thr Asn Phe Gln Met Asn Asp Lys Cys Ala
115 120 125
Ala Gly Thr Gly Arg Phe Leu Asp Val Met Ala Asn Ile Leu Glu Val
130 135 140
Lys Val Ser Asp Leu Ala Glu Leu Gly Ala Lys Ser Thr Lys Arg Val
145 150 155 160
Ala Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser
165 170 175
Gln Leu Ser Lys Gly Thr Asp Lys Ile Asp Ile Ile Ala Gly Ile His
180 185 190
Arg Ser Val Ala Ser Arg Val Ile Gly Leu Ala Asn Arg Val Gly Ile
195 200 205
Val Lys Asp Val Val Met Thr Gly Gly Val Ala Gln Asn Tyr Gly Val
210 215 220
Arg Gly Ala Leu Glu Glu Gly Leu Gly Val Glu Ile Lys Thr Ser Pro
225 230 235 240
Leu Ala Gln Tyr Asn Gly Ala Leu Gly Ala Ala Leu Tyr Ala Tyr Lys
245 250 255
Lys Ala Ala Lys
260
<210> 79
<211> 336
<212> PRT
<213> Carboxydothermus hydrogenoformans
<400> 79
Met Lys Leu Asn Tyr Phe Cys Ser Tyr Trp Pro Val Glu Ile Ser Glu
1 5 10 15
Gly Ala Gly Ile Ser Thr Val Arg Tyr Phe Pro Ser Asp Glu Ser Lys
20 25 30
Ala Pro Val Arg Leu Pro Ala Tyr Cys Cys Ser Tyr Ala Arg Gly Ser
35 40 45
Leu Ala Glu Ile Glu Glu Glu Gly Asp Gly Asp Phe Trp Gly Phe Ala
50 55 60
His Ser Cys Asp Thr Met Gln Ser Leu Tyr Gly Ile Thr Lys Ser Leu
65 70 75 80
Leu Gly Asp Asp Arg Val Phe Leu Phe Val Pro Pro Val Asp Leu Thr
85 90 95
Thr Ala Phe Ala Arg Glu Tyr Tyr Arg Glu Ala Leu Ile Tyr Leu Trp
100 105 110
Arg Glu Leu Ser Gln Lys Ser Gly Val Asn Gly Glu Glu Lys Leu Lys
115 120 125
Leu Thr Trp Glu Lys Leu Lys Glu Leu Arg Asn Lys Val Lys Ser Leu
130 135 140
Glu Asn Leu Thr Ser Ile Ile Pro Ser Ser Glu Ile Phe Glu Leu Leu
145 150 155 160
Lys Lys Leu Gln Thr Leu Pro Leu Asp Glu Ala Leu Asp Tyr Leu Glu
165 170 175
Ala Lys Lys Ala Glu Phe Thr Ser Leu Ser Val Ala Gln Lys Ala Ile
180 185 190
Gly Ile Ile Leu Thr Gly Ala Val Val Thr Asn Ser Lys Leu Tyr Leu
195 200 205
Ala Leu Glu Gln Gln Gly Phe Arg Val Val Tyr Asp Asp Thr Cys Thr
210 215 220
Gly Phe Arg His Phe Ala Gly Glu Ile Glu Asp Lys Asp Asp Ile Leu
225 230 235 240
Glu Ala Ile Val Ser Tyr Tyr Leu Ser Lys Pro Pro Cys Pro Cys Arg
245 250 255
His Lys Gly Val Trp Ala Arg Ala Glu Tyr Leu Lys Asn Leu Tyr His
260 265 270
Asn Lys Asn Ala Arg Ala Ile Val Leu Leu Gln Asn Lys Phe Cys Asp
275 280 285
Pro Phe Ala Trp Asp Val Pro Tyr Leu Val Asp Tyr Phe Lys Lys Gln
290 295 300
Gly Val Pro Val Leu Val Leu Glu Val Glu Gly Gly Glu Ile Gly Glu
305 310 315 320
Gln Asn Lys Thr Arg Leu Gln Ala Phe Arg Glu Ser Val Gly Gly Val
325 330 335
<210> 80
<211> 404
<212> PRT
<213> Carboxydothermus hydrogenoformans
<400> 80
Met Ala Lys Lys Ile Phe Lys Pro Leu Lys Ala Ser Glu Lys Ile Asn
1 5 10 15
Lys Ile Leu Lys Asn His Tyr Leu Lys Ala Lys Tyr Leu Pro Thr Leu
20 25 30
Gly Lys Phe Phe Gly Tyr Lys Thr Ala Trp Ile Thr Ser Gly Ala Pro
35 40 45
Val Glu Leu Leu Arg Ala Phe Gly Ile Glu Pro Val Tyr Pro Glu Asn
50 55 60
Tyr Gly Ala Ile Cys Gly Ala Arg Lys Val Ser Pro Ser Leu Cys Gln
65 70 75 80
Val Ala Glu Asn Arg Gly Tyr Ser Leu Asp Leu Cys Ser Tyr Ala Lys
85 90 95
Ser Asn Leu Gly Ser Ile Trp Asn Pro Lys Glu Ser Pro Phe Asn Gly
100 105 110
Leu Pro Arg Pro Asp Leu Leu Val Val Cys Asn Asn Ile Cys Gly Thr
115 120 125
Val Leu Lys Trp Tyr Glu Thr Leu Ser Arg Glu Phe Asn Ile Pro Leu
130 135 140
Phe Ile Ile Asp Thr Pro Phe Ile Thr Gly Glu Pro Gln Pro Trp Gln
145 150 155 160
Ile Gln Tyr Val Ala Lys Gln Ile Glu Lys Leu Ala Ile Glu Leu Glu
165 170 175
Lys Phe Phe Arg Lys Lys Leu Asp Leu Asn Arg Leu Glu Lys Val Ile
180 185 190
Leu Leu Ala Asn Glu Thr Val Asp Leu Trp Lys Gly Ile Arg Asn Phe
195 200 205
Ala Lys Asn Lys Pro Ser Pro Val Asn Val Thr Asp Leu Phe Ile Asn
210 215 220
Leu Gly Pro Met Val Val Leu Arg Gly Thr Glu Val Ala Arg Asp Phe
225 230 235 240
Tyr Glu Glu Val Tyr Arg Glu Val Glu Glu Arg Tyr Lys Ala Gly Val
245 250 255
Pro Ala Val Glu Gly Glu Lys Tyr Arg Leu Val Trp Asp Asn Ile Pro
260 265 270
Ile Trp Tyr Gly Leu Tyr Arg Phe Tyr Gly Tyr Phe Ala Glu Arg Gly
275 280 285
Ala Val Phe Val Thr Asp Ser Tyr Thr Gly Gly Trp Ala Val Asn Ile
290 295 300
Lys Lys Gly Pro Pro Phe Tyr Ala Leu Ala Glu Thr Tyr Ala Gly Val
305 310 315 320
Phe Leu Asn Arg Asp Leu Glu Phe Arg Lys Asn Gln Leu Gln Ser Phe
325 330 335
Ile Glu Glu Phe Ser Ala Asp Gly Phe Val Met His Ser Asn Arg Ser
340 345 350
Cys Lys Ala Tyr Ser Phe Val Gln Glu Glu Ile Arg Arg Gln Ile Met
355 360 365
Arg Ser Leu Gly Val Pro Gly Leu Ile Val Asp Ala Asp Met Thr Asp
370 375 380
Ser Arg Leu Tyr Ser Glu Glu Thr Val Leu Asn Arg Val Gln Ala Phe
385 390 395 400
Leu Glu Ser Leu
<210> 81
<211> 254
<212> PRT
<213> Carboxydothermus hydrogenoformans
<400> 81
Met Tyr Leu Gly Val Asp Ile Gly Ser Leu Thr Thr Lys Val Val Leu
1 5 10 15
Ile Asp Arg Gly Lys Asn Leu Ile Ala Tyr Arg Tyr Ser Lys Thr Gly
20 25 30
Pro Ala Gly Lys Glu Thr Ala Glu Arg Leu Ile Gln Glu Val Leu Ile
35 40 45
Lys Ala Asn Ile Ser Arg Asp Asp Ile Gln Gly Ile Val Ala Thr Gly
50 55 60
Tyr Gly Arg Val Leu Phe Ser Gly Lys Glu Phe Ser Glu Ile Thr Cys
65 70 75 80
Gln Ala Arg Gly Ile Gly His Leu Tyr Pro Glu Ala Lys Thr Ile Ile
85 90 95
Asp Ile Gly Gly Gln Asp Ser Lys Val Ile Ser Leu Gly Lys Asn Gly
100 105 110
Lys Val Leu Asp Phe Ala Met Asn Asp Lys Cys Ala Ala Gly Thr Gly
115 120 125
Arg Phe Leu Glu Val Met Ser Gln Ala Leu Glu Val Arg Leu Glu Glu
130 135 140
Ile Gly Glu Leu Ala Glu Lys Ser Gln Glu Ala Ala Lys Ile Ser Ser
145 150 155 160
Val Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Asn Leu Ser Arg
165 170 175
Gly Gln Ser Arg Glu Ala Val Ala Arg Gly Ile Cys Glu Ala Val Ala
180 185 190
Ala Arg Thr Ala Ile Leu Ala Gln Lys Val Gly Val Val Glu Pro Val
195 200 205
Val Phe Thr Gly Gly Val Ala Lys Asn Thr Gly Val Val Ala Ala Leu
210 215 220
Glu Arg Lys Leu Gly Val Lys Leu Leu Ile Pro Glu Asp Ser Thr Ile
225 230 235 240
Thr Ala Ala Leu Gly Ala Ala Leu Leu Ala Ala Glu Asn Ser
245 250
<210> 82
<211> 261
<212> PRT
<213> Oscillibacter valericigenes
<400> 82
Met Asn Asn Ile Tyr Thr Met Gly Ile Asp Val Gly Ser Thr Ala Ser
1 5 10 15
Lys Cys Leu Ile Leu Lys Asp Gly Ser Glu Ile Val Ala Lys Ser Leu
20 25 30
Val Asp Val Gly Ala Gly Thr Ser Gly Pro Thr Arg Ala Ile Ala Glu
35 40 45
Val Leu Glu Ala Ala Gly Met Lys Lys Glu Asp Met Ala Phe Ile Leu
50 55 60
Ala Thr Gly Tyr Gly Arg Asn Ser Leu Asp Asp Ile Ala Asp His Gln
65 70 75 80
Met Ser Glu Leu Ser Cys His Ala Lys Gly Ala Phe Phe Leu Phe Pro
85 90 95
Asp Val His Thr Val Ile Asp Ile Gly Gly Gln Asp Val Lys Ile Leu
100 105 110
Glu Ile Glu Asn Gly Val Met Val Asn Phe Ala Met Asn Asp Lys Cys
115 120 125
Ala Ala Gly Thr Gly Arg Phe Leu Asp Val Met Ala Arg Val Leu Glu
130 135 140
Val Lys Val Glu Asp Leu Ala Asp Leu Gly Ala Gln Ser Thr Lys Asn
145 150 155 160
Val Glu Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile
165 170 175
Ser Gln Leu Ala Lys Gly Ser Asp Lys Arg Asp Ile Ile His Gly Ile
180 185 190
His Lys Ser Val Ala Ser Arg Val Val Gly Leu Ala Asn Arg Ile Gly
195 200 205
Val Arg Asp Ala Val Val Met Thr Gly Gly Val Ala Gln Asn Gly Gly
210 215 220
Val Val Ser Ala Leu Gln Glu Ala Leu Gly His Pro Ile His Thr Ser
225 230 235 240
Pro Leu Thr Gln Tyr Asn Gly Ala Leu Gly Ala Ala Leu Phe Ala Trp
245 250 255
Gln Lys Ala Thr Lys
260
<210> 83
<211> 427
<212> PRT
<213> Oscillibacter valericigenes
<400> 83
Met Ala Glu Asn Glu Lys Ala Thr Ala Ala Ala Pro Glu Ala Ala Pro
1 5 10 15
Val Lys Lys Ala Pro Lys Pro Val Ser Pro Gly Thr Gln Ala Leu Arg
20 25 30
Asp Val Val Thr Lys Val Tyr Ala Ala Ala Trp Asp Ala Lys Lys Ala
35 40 45
Gly Arg Pro Val Gly Trp Ser Ser Ser Lys Phe Pro Cys Glu Ile Ala
50 55 60
Glu Ala Leu Gly Leu Ala Val Val Tyr Pro Glu Asn Gln Ala Ala Gly
65 70 75 80
Ile Gly Ala Gln His Asp Gly Gln Arg Met Cys Glu Ser Ala Glu Ser
85 90 95
Leu Gly Phe Asp Pro Asp Ile Cys Gly Tyr Ala Arg Ile Ser Leu Ala
100 105 110
Tyr Ser Ala Gly Val Glu Thr Thr Asn Glu Ser Arg Arg Val Pro Met
115 120 125
Pro Asp Phe Val Leu Cys Cys Asn Asn Ile Cys Asn Cys Met Thr Lys
130 135 140
Trp Tyr Glu Asn Ile Ala Arg Met His Asn Ile Pro Leu Ile Met Ile
145 150 155 160
Asp Val Pro Tyr Asn Asn Glu Val Thr Val Ser Asp Ser Gln Val Ala
165 170 175
Tyr Ile Arg Gly Gln Phe Asp Asp Ala Ile Lys Gln Met Glu Lys Ile
180 185 190
Ala Gly Val Lys Phe Asp Glu Lys Lys Phe Glu Gln Ala Cys Ala Asn
195 200 205
Ala Asn Arg Thr Ala Lys Ala Trp Leu Thr Val Cys Asp Tyr Leu Gln
210 215 220
Tyr Lys Pro Ala Pro Met Ser Gly Phe Asp Leu Phe Asn His Met Ala
225 230 235 240
Asp Val Val Thr Ala Arg Gly Lys Val Glu Thr Ala Glu Ala Phe Glu
245 250 255
Leu Leu Ala Ser Glu Leu Glu Gln His Val Lys Asn Gly Thr Ser Thr
260 265 270
Ala Pro Phe Pro Glu Gln Tyr Arg Val Met Phe Glu Gly Ile Pro Cys
275 280 285
Trp Pro Asn Leu Arg Thr Leu Phe Lys Pro Leu Lys Ala Asn Gly Val
290 295 300
Asn Val Thr Ala Val Val Tyr Ala Pro Ala Phe Gly Phe Val Tyr Asn
305 310 315 320
Gly Leu Asp Glu Met Ala Arg Ala Tyr Cys Lys Ala Pro Asn Ser Val
325 330 335
Cys Ile Glu Gln Gly Val Asp Trp Arg Glu Gly Ile Cys Arg Glu Asn
340 345 350
Lys Val Asp Gly Val Leu Val His Tyr Asn Arg Ser Cys Lys Pro Trp
355 360 365
Ser Gly Tyr Met Ala Glu Met Gln Arg Arg Phe Thr Lys Asp Leu Gly
370 375 380
Val Pro Cys Ala Gly Phe Asp Gly Asp Gln Ala Asp Pro Arg Asn Phe
385 390 395 400
Asn Glu Ala Gln Tyr Glu Thr Arg Val Gln Gly Leu Val Glu Ala Met
405 410 415
Glu Glu Asn Lys Lys Gln Lys Glu Ala Arg Ala
420 425
<210> 84
<211> 380
<212> PRT
<213> Oscillibacter valericigenes
<400> 84
Met Ser Ile Glu Thr Ile Val Lys Glu Phe Ala Asp Val Ala Ala Asp
1 5 10 15
Pro Lys Ala Gln Leu Lys Lys Tyr Lys Ala Glu Gly Lys Lys Cys Ile
20 25 30
Gly Val Met Pro Tyr Tyr Ala Pro Glu Glu Leu Val Ala Ala Ala Gly
35 40 45
Met Val Pro Phe Gly Met Trp Gly Ser Asn Asp Lys Thr Ile Ser Arg
50 55 60
Ala Lys Glu Tyr Cys Ala Thr Phe Tyr Cys Thr Ile Ala Gln Leu Asp
65 70 75 80
Leu Glu Met Leu Leu Asp Gly Thr Met Asp Leu Leu Asp Gly Val Ile
85 90 95
Thr Pro Thr Ile Cys Asp Thr Leu Arg Pro Met Ser Gln Asn Ile Arg
100 105 110
Val Ala Met Gly Glu Lys Leu Pro Cys Ile Phe Leu Ala His Pro Gln
115 120 125
Asn Arg Lys Pro Ala Tyr Gly Lys Lys Phe Cys Leu Asp Gln Tyr Thr
130 135 140
His Ile Lys Thr Glu Leu Glu Lys Ile Ala Gly Ala Pro Ile Thr Asp
145 150 155 160
Ala Ala Leu Ser Glu Thr Ile Lys Val Tyr Asn Lys Ser Arg Ala Ala
165 170 175
Arg Arg Glu Phe Val Lys Leu Val Ser Asp His Cys Asp Val Ile Thr
180 185 190
Pro Thr Lys Arg Ser Ala Val Leu Lys Ala Ala Trp Phe Met Pro Lys
195 200 205
Ala Glu Tyr Thr Glu Lys Leu Lys Ala Leu Asn Ala Glu Leu Lys Ala
210 215 220
Leu Pro Val Cys Asp Trp Lys Gly Thr Lys Val Val Thr Ser Gly Ile
225 230 235 240
Ile Cys Asp Asn Pro Lys Leu Leu Glu Ile Phe Glu Glu Asn Lys Ile
245 250 255
Ala Ile Ala Ala Asp Asp Val Ala His Glu Ser Arg Ser Phe Arg Val
260 265 270
Asp Ala Pro Glu Thr Gly Asp Pro Met Glu Ala Leu Ala Gln Gln Phe
275 280 285
Ala Asn Gln Asp Tyr Asp Val Leu Leu Tyr Asp Glu His Ser Ser Glu
290 295 300
Asn Arg Arg Gly Glu Phe Val Ala Lys Leu Val Lys Asp Ser Gly Ala
305 310 315 320
Lys Gly Leu Val Leu Phe Met Gln Gln Phe Cys Asp Pro Glu Glu Met
325 330 335
Glu Tyr Pro Ser Leu Lys Lys Ala Leu Asp Glu Ala Lys Ile Pro His
340 345 350
Ile Lys Leu Gly Val Asp Gln Gln Met Arg Asp Phe Gly Gln Ala Arg
355 360 365
Thr Ala Ile Gln Ala Phe Ala Asp Val Ile Ser Leu
370 375 380
<210> 85
<211> 422
<212> PRT
<213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628)
<400> 85
Met Thr Asp Thr Ala Asn Met Ser Ala Lys Glu Leu Leu Gly Phe Tyr
1 5 10 15
Gln Glu Glu Leu Tyr Glu Glu Ala Arg Gln Ala Lys Lys Glu Gly Lys
20 25 30
Leu Val Cys Trp Ser Ala Ser Val Ala Pro Ser Glu Phe Cys Val Ala
35 40 45
Met Asp Val Ala Met Ile Tyr Pro Glu Thr His Ala Ala Gly Ile Gly
50 55 60
Ala Arg Lys Gly Ala Leu Asp Met Leu Glu Val Ala Asp Glu Lys Gly
65 70 75 80
Tyr Asn Leu Asp Thr Cys Ser Tyr Ala Arg Val Asn Leu Gly Tyr Met
85 90 95
Glu Leu Leu Lys Gln Glu Ala Leu Thr Gly Ile Thr Pro Glu Lys Leu
100 105 110
Glu Lys Ser Pro Ala Ala Arg Val Pro Leu Pro Asp Phe Val Ile Thr
115 120 125
Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu Asn Leu Ala
130 135 140
Val Glu Leu Asn Ile Pro Cys Ile Val Ile Asp Val Pro Phe Asn His
145 150 155 160
Thr Met Pro Ile Pro Gln Tyr Ala Lys Asp Tyr Ile Ala Glu Gln Phe
165 170 175
Lys Glu Ala Ile Ala Gln Leu Glu Glu Ile Cys Gly Lys Lys Phe Asp
180 185 190
Tyr Asp Lys Phe Leu Gln Val Gln Glu Gln Thr Gln Arg Ser Val Ala
195 200 205
Gln Trp Asn Arg Ile Ala Ser Leu Ser Gly His Lys Pro Ser Pro Leu
210 215 220
Asn Gly Phe Asp Leu Phe Asn Tyr Met Ala Leu Ile Val Cys Ala Arg
225 230 235 240
Ser Arg Asp Cys Ala Glu Ile Thr Phe Lys Lys Phe Ala Asp Glu Leu
245 250 255
Glu Asp Asn Leu Ser Lys Gly Ile Tyr Ala Phe Lys Gly Asn Glu Gln
260 265 270
Lys Arg Ile Thr Trp Glu Gly Ile Ala Val Trp Pro His Leu Gly His
275 280 285
Thr Phe Lys Gly Leu Lys Asn Leu Gly Asn Ile Met Thr Gly Ser Ala
290 295 300
Tyr Pro Gly Leu Trp Asn Leu Ser Tyr Thr Pro Gly Asp Met Ser Ser
305 310 315 320
Met Ala Glu Ala Tyr Thr Arg Ile Tyr Ile Asn Thr Cys Leu Asp Asn
325 330 335
Lys Val Lys Val Leu Ser Asp Ile Ile Ser Gly Gly Lys Cys Asp Gly
340 345 350
Val Ile Tyr His Gln Asn Arg Ser Cys Lys Leu Met Ser Phe Leu Asn
355 360 365
Val Glu Thr Ala Asp Ile Leu Gln Gln Gln Asn His Leu Pro Tyr Val
370 375 380
Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ala Pro Ala Gln
385 390 395 400
Phe Asp Thr Arg Ile Gln Ala Leu Asp Glu Met Met Lys Gln Asn Lys
405 410 415
Glu Gly Val Ser His Glu
420
<210> 86
<211> 372
<212> PRT
<213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628)
<400> 86
Met Ser Arg Ile Glu Ala Ile Ile Ser Glu Leu Ser Ser Ile Ala Asn
1 5 10 15
Asn Pro Arg Lys Ala Met Glu Asp Tyr Lys Lys Glu Thr Gly Lys Gly
20 25 30
Ser Val Gly Ile Met Pro Tyr Tyr Ala Pro Glu Glu Ile Val His Ala
35 40 45
Ala Gly Tyr Leu Pro Val Gly Ile Trp Gly Gly Gln Lys Ser Ile Ser
50 55 60
Lys Ala Arg Ala Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln Ser
65 70 75 80
Val Val Glu Met Gln Leu Glu Gly Val Tyr Asn Asp Leu Ala Ala Val
85 90 95
Leu Phe Pro Val Pro Cys Asp Thr Leu Lys Cys Leu Ser Gln Lys Trp
100 105 110
Lys Gly Thr Ser Pro Val Ile Val Met Thr His Pro Gln Asn Arg Lys
115 120 125
Leu Glu Ala Ala Asn Lys Phe Leu Ala Glu Glu Tyr Arg Leu Val Arg
130 135 140
Glu Lys Leu Glu Lys Ile Leu Asn Val Gln Ile Thr Asp Glu Ala Leu
145 150 155 160
Asn His Ser Ile Asp Val Tyr Asn Glu Asn Arg Lys Ala Met Arg Glu
165 170 175
Phe Thr Asp Ile Ala Ala Asn Tyr Leu Asn Ile Ile Asp Pro Arg Lys
180 185 190
Arg His Glu Ile Ile Lys Ala Arg Phe Phe Met Glu Lys Ser Lys His
195 200 205
Thr Ala Leu Val Lys Glu Leu Asn Ser Glu Leu Lys Ser Leu Pro Val
210 215 220
Glu Asp Phe Thr Gly Lys Lys Val Ile Leu Thr Gly Ile Met Ala Glu
225 230 235 240
Pro Asn Glu Val Leu Asp Ile Leu Lys Glu Asn Asp Phe Ala Val Val
245 250 255
Ala Asp Asp Leu Ala Gln Glu Ser Arg Leu Phe Arg Ile Asp Val Pro
260 265 270
Ala Gly Pro Asp Pro Leu Tyr Arg Leu Ala Lys Trp Trp Gln Glu Phe
275 280 285
Asp Gly Cys Ser Leu Ala Val Asp Thr Lys Lys Leu Arg Gly Pro Met
290 295 300
Leu Met Asn Met Val Asn Val Asp Lys Ala Asp Ala Val Val Val Cys
305 310 315 320
Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Ile Tyr Tyr
325 330 335
Arg Gln Phe Glu Glu Ala Gly Ile Lys Ser Leu Phe Ile Glu Ile Asp
340 345 350
Leu Glu Pro Thr Ser Phe Glu Gln Thr Lys Thr Arg Val Gln Ser Phe
355 360 365
Arg Glu Met Leu
370
<210> 87
<211> 266
<212> PRT
<213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628)
<400> 87
Met Tyr Thr Met Gly Ile Asp Ile Gly Ser Ser Ser Ser Lys Val Val
1 5 10 15
Ile Leu Glu Asp Gly Val Asn Leu Ile Ala Gly Glu Val Ile Gln Ile
20 25 30
Gly Thr Gly Ser Thr Gly Pro Lys Arg Val Leu Glu Glu Ala Leu Ala
35 40 45
Lys Thr Gly Leu Thr Leu Ala Asp Met Ala Lys Ile Ile Ala Thr Gly
50 55 60
Tyr Gly Arg Ser Ser Val Glu Val Ser Asp Lys Gln Ile Ser Glu Ile
65 70 75 80
Ser Cys Gln Ala Lys Gly Val Tyr Phe Leu Val Pro Thr Ala Lys Leu
85 90 95
Ile Ile Asp Ile Gly Gly Gln Asp Val Lys Ala Ile Arg Leu Asp Arg
100 105 110
Ile Gly Gly Val Arg Gln Phe Phe Met Asn Asp Lys Cys Ala Ala Gly
115 120 125
Thr Gly Arg Phe Leu Asp Val Met Ser Arg Val Leu Glu Val Asp Leu
130 135 140
Asp Glu Met Ala Glu Tyr Asp Ala Arg Ala Thr Glu Pro Ala Thr Val
145 150 155 160
Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu
165 170 175
Ala Asn Gly Val Ala Lys Glu Asn Ile Ile Ala Gly Val His Gln Ser
180 185 190
Val Ala Ser Lys Ala Cys Gly Leu Ala Tyr Arg Cys Gly Val Glu Glu
195 200 205
Asp Val Val Met Cys Gly Gly Val Ala Lys Asp Leu Gly Val Val Arg
210 215 220
Ala Ile Ser Lys Glu Leu Lys Lys Pro Val Ile Val Ala Pro Asn Pro
225 230 235 240
Gln Ile Thr Ala Ala Leu Gly Ala Ala Leu Phe Ala Tyr Glu Glu Val
245 250 255
Met Glu Ala Asn Lys Leu Arg Lys Glu Val
260 265
<210> 88
<211> 411
<212> PRT
<213> Peptostreptococcus anaerobius CAG:621
<400> 88
Met Ser Asn Thr Gly Ala Val Glu Glu Lys Pro Ala Lys Val Leu Leu
1 5 10 15
Gly Glu Ile Val Ala Lys His Tyr Lys Glu Ala Trp Glu Ala Lys Glu
20 25 30
Arg Gly Glu Lys Val Gly Trp Cys Ala Ser Asn Phe Pro Gln Glu Ile
35 40 45
Phe Glu Thr Met Asp Ile Lys Val Val Phe Pro Glu Asn Gln Ala Ala
50 55 60
Ala Ile Ser Ala Lys Gly Gly Gly Gln Arg Met Cys Glu Ile Ala Glu
65 70 75 80
Asn Glu Gly Tyr Ser Asn Asp Ile Cys Ala Tyr Ala Arg Ile Ser Leu
85 90 95
Ala Tyr Met Asp Val Lys Asp Ala Pro Glu Leu Asn Met Pro Gln Pro
100 105 110
Asp Phe Val Ala Cys Cys Asn Asn Ile Cys Asn Cys Met Ile Lys Trp
115 120 125
Tyr Glu Asn Ile Ala Lys Glu Leu Asn Ile Pro Leu Ile Leu Val Asp
130 135 140
Val Pro Tyr Asn Asn Asp Tyr Glu Ala Gly Asp Asp Arg Val Glu Tyr
145 150 155 160
Leu Arg Gly Gln Phe Asp His Ala Ile Lys Gln Leu Glu Asp Leu Thr
165 170 175
Gly Lys Lys Trp Asp Glu Lys Lys Phe Glu Glu Val Met Ala Ile Ser
180 185 190
Gln Arg Thr Gly Arg Ala Trp Leu Lys Ala Thr Gly Tyr Ala Lys Tyr
195 200 205
Thr Pro Ser Pro Phe Ser Gly Phe Asp Val Phe Asn His Met Ala Val
210 215 220
Ala Val Cys Ala Arg Gly Lys Glu Glu Ser Ala Ile Ala Phe Glu Lys
225 230 235 240
Leu Ala Glu Glu Phe Asp Glu Asn Val Lys Thr Gly Lys Ser Thr Phe
245 250 255
Lys Gly Glu Glu Lys Tyr Arg Val Leu Phe Glu Gly Ile Ala Cys Trp
260 265 270
Pro His Leu Arg His Thr Phe Lys Gln Leu Lys Asp Ser Gly Val Asn
275 280 285
Val Cys Gly Thr Val Tyr Ala Asp Ala Phe Gly Tyr Ile Tyr Asp Asn
290 295 300
Thr Tyr Glu Leu Met Gln Ala Tyr Cys Gly Thr Pro Asn Ala Ile Ser
305 310 315 320
Tyr Glu Arg Ser Leu Asp Met Arg Leu Lys Val Ile Glu Glu Asn Asn
325 330 335
Ile Asp Gly Met Leu Ile His Ile Asn Arg Ser Cys Lys Gln Trp Ser
340 345 350
Gly Ile Met Tyr Glu Met Glu Arg Glu Ile Arg Glu Arg Thr Gly Ile
355 360 365
Pro Thr Ala Thr Phe Asp Gly Asp Gln Ala Asp Pro Arg Asn Phe Ser
370 375 380
Glu Ala Gln Tyr Asp Thr Arg Val Gln Gly Leu Ile Glu Val Met Glu
385 390 395 400
Ala Asn Lys Ala Ala Lys Met Lys Glu Glu Asn
405 410
<210> 89
<211> 372
<212> PRT
<213> Peptostreptococcus anaerobius CAG:621
<400> 89
Met Ser Asn Leu Glu Glu Leu Phe Gly Lys Leu Ala Val Cys Pro Leu
1 5 10 15
Glu Gln Ile Asp Lys Tyr Val Ala Asp Gly Lys Lys Val Ile Gly Cys
20 25 30
Ala Pro Val Tyr Ala Pro Glu Glu Leu Val Tyr Ala Ser Gly Met Ile
35 40 45
Pro Met Ala Ile Trp Gly Ala Glu Gly Glu Val Thr Leu Ala Lys Glu
50 55 60
Tyr Phe Pro Ala Phe Tyr Val Ser Ile Ile Leu Arg Leu Leu Asp Leu
65 70 75 80
Gly Leu Glu Gly Lys Leu Asp Lys Met Ser Gly Met Ile Leu Pro Gly
85 90 95
Leu Ser Asp Gly Leu Lys Gly Leu Ser Gln Asn Trp Lys Arg Ala Val
100 105 110
Lys Asn Val Pro Ala Leu Tyr Ile Gly Tyr Gly Gln Asn Arg Lys Ile
115 120 125
Glu Ala Gly Ile Val Tyr Asn Ala Arg Gln Tyr Glu Lys Leu Lys Val
130 135 140
Gln Leu Glu Glu Ile Ala Gly Lys Lys Ile Glu Asp Ala Gln Ile Glu
145 150 155 160
Glu Ala Ile Val Leu Tyr Asn Lys His Arg Lys Ala Met Gln Ala Phe
165 170 175
Ser Asp Leu Ala Ala Lys His Leu Asn Thr Val Thr Pro Ser Leu Arg
180 185 190
Ala Lys Val Met Ser Ser Ala Cys Leu Met Asp Lys Ala Glu His Leu
195 200 205
Glu Ile Val Glu Ala Ile Asn Ala Glu Leu Ser Ala Met Pro Glu Glu
210 215 220
Lys Phe Asp Gly Lys Lys Ile Val Thr Thr Gly Leu Leu Ala Asn Ser
225 230 235 240
Pro Glu Ile Leu Lys Ile Phe Glu Glu Phe Lys Leu Gly Ile Val Ala
245 250 255
Asp Asn Ile Asn His Glu Ser Gly Gln Phe Asp Tyr Leu Val Asp Glu
260 265 270
Ala Thr Gly Asn Pro Ile Lys Ala Leu Ser Lys Trp Ile Ser Asp Ile
275 280 285
Glu Gly Ser Thr Leu Leu Tyr Asp Pro Glu Lys Leu Arg Gly Gln Ile
290 295 300
Ile Ile Asp Lys Ala Lys Lys Tyr Asp Ala Asp Gly Val Val Tyr Leu
305 310 315 320
Leu Ser Lys Phe Ser Asp Ser Asp Glu Phe Asp Tyr Pro Ile Ile Arg
325 330 335
Lys Gln Leu Glu Glu Ala Gly Tyr Met His Ile Leu Val Glu Val Asp
340 345 350
Gln Gln Met Thr Asn Phe Glu Gln Ala Lys Thr Ala Leu Gln Thr Phe
355 360 365
Ala Asp Met Ile
370
<210> 90
<211> 263
<212> PRT
<213> Peptostreptococcus anaerobius CAG:621
<400> 90
Met Ser Asp Ile Tyr Thr Met Gly Ile Asp Ile Gly Ser Thr Ser Ser
1 5 10 15
Lys Cys Val Val Leu Lys Asn Gly Lys Asp Leu Val Ser Ser Gly Val
20 25 30
Val Asn Leu Gly Ala Gly Thr Lys Gly Ala Asp Gln Val Ile Glu Lys
35 40 45
Val Leu Ala Asp Cys Gly Ile Lys Phe Glu Asp Leu Asn Val Ile Val
50 55 60
Ser Thr Gly Tyr Gly Arg Asn Ser Tyr Asp Ser Ala Lys Lys Thr Met
65 70 75 80
Ser Glu Leu Ser Cys His Ala Lys Gly Gly Thr Tyr Ile Phe Gly Pro
85 90 95
Val Arg Thr Ile Ile Asp Ile Gly Gly Gln Asp Ile Lys Val Leu Lys
100 105 110
Leu Asn Asp Lys Gly Met Met Thr Asn Phe Leu Met Asn Asp Lys Cys
115 120 125
Ala Ala Gly Thr Gly Arg Phe Leu Glu Val Met Ala Gly Val Leu Asp
130 135 140
Val Lys Leu Ala Glu Leu Gly Asp Leu Asp Lys Leu Ala Thr Glu Lys
145 150 155 160
Thr Pro Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile
165 170 175
Ser Cys Met Ala Lys Lys Ile Pro Ile Pro Asn Ile Ile Arg Gly Ile
180 185 190
His Ala Ser Val Ala Thr Arg Val Ala Gly Leu Ala Lys Arg Gly Gly
195 200 205
Leu Thr Thr Pro Val Ala Met Thr Gly Gly Val Thr Lys Asn Ser Gly
210 215 220
Ile Val Arg Ala Leu Ser Glu Glu Leu Glu Thr Asp Ile Met Ile Ser
225 230 235 240
Glu Ile Ser Gln Leu Ala Gly Ala Ile Gly Ala Ala Leu Tyr Ala Tyr
245 250 255
Asp Glu Tyr Leu Lys Glu Asn
260
<210> 91
<211> 258
<212> PRT
<213> Chloroflexus aggregans (strain MD-66 / DSM 9485)
<400> 91
Met Ser Asp Glu Thr Leu Val Leu Ser Thr Ile Glu Gly Pro Val Ala
1 5 10 15
Ile Leu Thr Leu Asn Arg Pro Gln Ala Leu Asn Ala Leu Ser Pro Ala
20 25 30
Leu Ile Asp Ala Leu Ile Arg His Leu Glu His Cys Asp Asn Asp Asp
35 40 45
Thr Ile Arg Val Ile Ile Ile Thr Gly Ala Gly Arg Ala Phe Ala Ala
50 55 60
Gly Ala Asp Ile Lys Ala Met Ala Asp Ala Thr Pro Ile Asp Met Leu
65 70 75 80
Thr Thr Asp Met Ile Ala Arg Trp Ala Arg Ile Ala Ala Val Arg Lys
85 90 95
Pro Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly Cys Glu
100 105 110
Leu Ala Met Met Cys Asp Ile Ile Leu Ala Ser Glu Thr Ala Gln Phe
115 120 125
Gly Gln Pro Glu Ile Asn Ile Gly Ile Ile Pro Gly Ala Gly Gly Thr
130 135 140
Gln Arg Leu Thr Arg Ala Ile Gly Pro Tyr Arg Ala Met Glu Met Val
145 150 155 160
Leu Thr Gly Ala Thr Ile Ser Ala Gln Glu Ala Tyr Ala Tyr Gly Leu
165 170 175
Val Asn Arg Val Cys Pro Pro Asp Ser Leu Leu Asp Glu Ala Arg Arg
180 185 190
Leu Ala Gln Thr Ile Ala Ala Lys Pro Pro Leu Ala Val Arg Leu Ala
195 200 205
Lys Glu Ala Val Arg Ala Ala Ala Glu Thr Thr Val Arg Glu Gly Leu
210 215 220
Ala Ile Glu Leu Arg Asn Phe Tyr Leu Leu Phe Ala Ser Ala Asp Gln
225 230 235 240
Lys Glu Gly Met Arg Ala Phe Ile Glu Lys Arg Thr Ala Asn Phe Ser
245 250 255
Gly Arg
<210> 92
<211> 257
<212> PRT
<213> Marivirga tractuosa
<400> 92
Met Glu Phe Ile Lys Val Asn Thr Gln Tyr Lys Lys His Ile Ala Leu
1 5 10 15
Ile Asn Leu Asn Arg Pro Lys Glu Leu Asn Ala Leu Asn Leu Gln Leu
20 25 30
Met Thr Glu Leu Lys Asp Thr Leu Lys Val Leu Asp Glu Asp Glu Asn
35 40 45
Val Arg Val Ile Ile Leu Thr Gly Asn Glu Lys Ala Phe Ala Ala Gly
50 55 60
Ala Asp Ile Lys Gln Met Ala Gly Lys Thr Ala Ile Asp Met Leu Asn
65 70 75 80
Val Asp Gln Phe Ser Thr Trp Asp Gln Ile Lys Lys Thr Lys Lys Pro
85 90 95
Leu Ile Ala Ala Val Ser Gly Phe Ala Leu Gly Gly Gly Cys Glu Leu
100 105 110
Ala Met Thr Cys Asp Met Ile Val Ala Ser Glu Ser Ala Lys Phe Gly
115 120 125
Gln Pro Glu Ile Lys Ile Gly Val Met Pro Gly Ala Gly Gly Thr Gln
130 135 140
Arg Leu Thr Arg Ala Ile Gly Lys Ala Lys Ala Met Glu Leu Val Leu
145 150 155 160
Thr Gly Asn Phe Ile Ser Ala Glu Glu Ala Met His Tyr Gly Leu Val
165 170 175
Asn Lys Val Val Pro Thr Glu Met Tyr Leu Glu Ala Ala Ala Glu Leu
180 185 190
Ala Glu Gln Ile Ala Gln Met Ser Pro Val Ala Ala Lys Leu Ala Lys
195 200 205
Glu Ser Val Asn Arg Ala Phe Glu Thr His Leu Asp Glu Gly Leu His
210 215 220
Phe Glu Arg Lys Asn Phe Tyr Leu Thr Phe Ala Ser Glu Asp Gln Thr
225 230 235 240
Glu Gly Met Glu Ala Phe Val Glu Lys Arg Lys Pro Glu Phe Lys Gly
245 250 255
Lys
<210> 93
<211> 257
<212> PRT
<213> Marinithermus hydrothermalis (strain DSM 14884/JCM 11576/T1)
<400> 93
Met Tyr Glu Asn Leu Ile Val Glu Thr Leu Glu Gly Gly Val Gly Leu
1 5 10 15
Ile Arg Ile His Arg Pro Lys Arg Leu Asn Ala Leu Asn Gln Ala Thr
20 25 30
Met Asp Glu Ile Val Arg Ala Val Arg Ala Phe Glu Ala Asp Asp Ala
35 40 45
Val Arg Ala Ile Val Leu Thr Gly Asp Glu Arg Ala Phe Ala Ala Gly
50 55 60
Ala Asp Val Thr Glu Met Asp Gly Ala Asn Val Pro Glu Met Leu Ser
65 70 75 80
Gly Tyr Arg Phe Glu Gln Trp Glu Thr Leu Arg Arg Thr Thr Lys Pro
85 90 95
Leu Ile Ala Ala Val Ser Gly Phe Ala Leu Gly Gly Gly Leu Glu Leu
100 105 110
Ala Met Leu Cys Asp Ile Ile Val Ala Ser Glu Thr Ala Arg Leu Gly
115 120 125
Gln Pro Glu Ile Asn Leu Gly Ile Met Pro Gly Ala Gly Gly Thr Gln
130 135 140
Arg Leu Thr Arg Gln Val Gly Lys Tyr Leu Ala Met Glu Met Val Leu
145 150 155 160
Thr Gly Arg Met Leu Thr Ala Glu Glu Ala Tyr Arg His Gly Leu Val
165 170 175
Asn Arg Val Val Pro Val Glu Phe Tyr Leu Glu Glu Ala Ile Gln Ile
180 185 190
Ala Arg Glu Ile Ala Lys Lys Ala Pro Val Ala Val Arg Leu Ala Lys
195 200 205
Asp Ala Ile Leu Lys Ala Glu Asp Thr Pro Leu Glu Val Gly Leu Ala
210 215 220
Tyr Glu Arg His Asn Phe Tyr Leu Leu Phe Gly Thr Glu Asp Lys Gln
225 230 235 240
Glu Gly Ile Arg Ala Phe Leu Glu Lys Arg Lys Pro Glu Trp Lys Gly
245 250 255
Arg
<210> 94
<211> 259
<212> PRT
<213> Chitinophaga pinensis (strain ATCC 43595/DSM 2588/NCIB11800/UQM 2034)
<400> 94
Met Gln Pro Gln Phe Ile Ile Ile His Arg Gln Val Ala Pro Tyr Val
1 5 10 15
Ala His Ile Gln Leu Asn Arg Pro Lys Glu Leu Asn Ala Leu Asn Leu
20 25 30
Glu Leu Met Ile Glu Leu Arg Asp Ala Leu Lys Met Leu Asp Ala Asp
35 40 45
Asp Asn Val Arg Ala Ile Val Ile Ser Gly Asn Glu Lys Ala Phe Ala
50 55 60
Ala Gly Ala Asp Ile Lys Gln Met Ala Gly Lys Thr Ala Met Asp Met
65 70 75 80
Tyr Asn Ile Asp Gln Phe Ser Thr Trp Asp Thr Ile Lys Lys Thr Lys
85 90 95
Lys Pro Leu Ile Ala Ala Val Ser Gly Phe Ala Leu Gly Gly Gly Cys
100 105 110
Glu Leu Val Met Leu Cys Asp Met Ile Val Ala Ser Glu Thr Ala Arg
115 120 125
Phe Gly Gln Pro Glu Ile Lys Ile Gly Val Met Pro Gly Ala Gly Gly
130 135 140
Thr Gln Arg Leu Thr Arg Ala Val Gly Lys Ala Leu Ala Met Glu Met
145 150 155 160
Val Leu Thr Gly Arg Phe Ile Thr Ala Gln Glu Ala Ala Arg Ala Gly
165 170 175
Leu Ile Asn Arg Val Ile Pro Val Glu Leu Phe Leu Gln Glu Ala Ile
180 185 190
Arg Leu Ala Thr Glu Val Ala Ala Leu Ser Pro Leu Ala Val Lys Met
195 200 205
Ala Lys Glu Ser Val Leu Lys Ala Phe Asp Ser Ser Leu Glu Glu Gly
210 215 220
Leu His Phe Glu Arg Lys Asn Phe Tyr Leu Leu Phe Ala Ser Glu Asp
225 230 235 240
Gln Lys Glu Gly Met Gln Ala Phe Val Asp Lys Arg Ser Pro Val Phe
245 250 255
Lys Gly Lys
<210> 95
<211> 258
<212> PRT
<213> Megasphaera elsdenii DSM 20460
<400> 95
Met Tyr Thr Leu Gly Ile Asp Val Gly Ser Ser Ser Ser Lys Ala Val
1 5 10 15
Ile Leu Glu Asp Gly Lys Lys Ile Val Ala His Ala Val Val Glu Ile
20 25 30
Gly Thr Gly Ser Thr Gly Pro Glu Arg Val Leu Asp Glu Val Phe Lys
35 40 45
Asp Thr Asn Leu Lys Ile Glu Asp Met Ala Asn Ile Ile Ala Thr Gly
50 55 60
Tyr Gly Arg Phe Asn Val Asp Cys Ala Lys Gly Glu Val Ser Glu Ile
65 70 75 80
Thr Cys His Ala Lys Gly Ala Leu Phe Glu Cys Pro Gly Thr Thr Thr
85 90 95
Ile Leu Asp Ile Gly Gly Gln Asp Val Lys Ser Ile Lys Leu Asn Gly
100 105 110
Gln Gly Leu Val Met Gln Phe Ala Met Asn Asp Lys Cys Ala Ala Gly
115 120 125
Thr Gly Arg Phe Leu Asp Val Met Ser Lys Val Leu Glu Ile Pro Met
130 135 140
Ser Glu Met Gly Asp Trp Tyr Phe Lys Ser Lys His Pro Ala Ala Val
145 150 155 160
Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Leu Leu
165 170 175
Ser Lys Asn Val Pro Lys Glu Asp Ile Val Ala Gly Val His Gln Ser
180 185 190
Ile Ala Ala Lys Ala Cys Ala Leu Val Arg Arg Val Gly Val Gly Glu
195 200 205
Asp Leu Thr Met Thr Gly Gly Gly Ser Arg Asp Pro Gly Val Val Asp
210 215 220
Ala Val Ser Lys Glu Leu Gly Ile Pro Val Arg Val Ala Leu His Pro
225 230 235 240
Gln Ala Val Gly Ala Leu Gly Ala Ala Leu Ile Ala Tyr Asp Lys Ile
245 250 255
Lys Lys
<210> 96
<211> 428
<212> PRT
<213> Megasphaera elsdenii DSM 20460
<400> 96
Met Ser Glu Glu Lys Thr Val Asp Ile Glu Ser Met Ser Ser Lys Glu
1 5 10 15
Ala Leu Gly Tyr Phe Leu Pro Lys Val Asp Glu Asp Ala Arg Lys Ala
20 25 30
Lys Lys Glu Gly Arg Leu Val Cys Trp Ser Ala Ser Val Ala Pro Pro
35 40 45
Glu Phe Cys Thr Ala Met Asp Ile Ala Ile Val Tyr Pro Glu Thr His
50 55 60
Ala Ala Gly Ile Gly Ala Arg His Gly Ala Pro Ala Met Leu Glu Val
65 70 75 80
Ala Glu Asn Lys Gly Tyr Asn Gln Asp Ile Cys Ser Tyr Cys Arg Val
85 90 95
Asn Met Gly Tyr Met Glu Leu Leu Lys Gln Gln Ala Leu Thr Gly Glu
100 105 110
Thr Pro Glu Val Leu Lys Asn Ser Pro Ala Ser Pro Ile Pro Leu Pro
115 120 125
Asp Val Val Leu Thr Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp
130 135 140
Tyr Glu Asn Leu Ala Lys Glu Leu Asn Val Pro Leu Ile Asn Ile Asp
145 150 155 160
Val Pro Phe Asn His Glu Phe Pro Val Thr Lys His Ala Lys Gln Tyr
165 170 175
Ile Val Gly Glu Phe Lys His Ala Ile Lys Gln Leu Glu Asp Leu Cys
180 185 190
Gly Arg Pro Phe Asp Tyr Asp Lys Phe Phe Glu Val Gln Lys Gln Thr
195 200 205
Gln Arg Ser Ile Ala Ala Trp Asn Lys Ile Ala Thr Tyr Phe Gln Tyr
210 215 220
Lys Pro Ser Pro Leu Asn Gly Phe Asp Leu Phe Asn Tyr Met Gly Leu
225 230 235 240
Ala Val Ala Ala Arg Ser Leu Asn Tyr Ser Glu Ile Thr Phe Asn Lys
245 250 255
Phe Leu Lys Glu Leu Asp Glu Lys Val Ala Asn Lys Lys Trp Ala Phe
260 265 270
Gly Glu Asn Glu Lys Ser Arg Val Thr Trp Glu Gly Ile Ala Val Trp
275 280 285
Ile Ala Leu Gly His Thr Phe Lys Glu Leu Lys Gly Gln Gly Ala Leu
290 295 300
Met Thr Gly Ser Ala Tyr Pro Gly Met Trp Asp Val Ser Tyr Glu Pro
305 310 315 320
Gly Asp Leu Glu Ser Met Ala Glu Ala Tyr Ser Arg Thr Tyr Ile Asn
325 330 335
Cys Cys Leu Glu Gln Arg Gly Ala Val Leu Glu Lys Val Val Arg Asp
340 345 350
Gly Lys Cys Asp Gly Leu Ile Met His Gln Asn Arg Ser Cys Lys Asn
355 360 365
Met Ser Leu Leu Asn Asn Glu Gly Gly Gln Arg Ile Gln Lys Asn Leu
370 375 380
Gly Val Pro Tyr Val Ile Phe Asp Gly Asp Gln Thr Asp Ala Arg Asn
385 390 395 400
Phe Ser Glu Ala Gln Phe Asp Thr Arg Val Glu Ala Leu Ala Glu Met
405 410 415
Met Ala Asp Lys Lys Ala Asn Glu Gly Gly Asn His
420 425
<210> 97
<211> 372
<212> PRT
<213> Megasphaera elsdenii DSM 20460
<400> 97
Met Ser Gln Ile Asp Glu Leu Ile Ser Lys Leu Gln Glu Val Ser Asn
1 5 10 15
His Pro Gln Lys Thr Val Leu Asn Tyr Lys Lys Gln Gly Lys Gly Leu
20 25 30
Val Gly Met Met Pro Tyr Tyr Ala Pro Glu Glu Ile Val Tyr Ala Ala
35 40 45
Gly Tyr Leu Pro Val Gly Met Phe Gly Ser Gln Asn Pro Gln Ile Ser
50 55 60
Ala Ala Arg Thr Tyr Leu Pro Pro Phe Ala Cys Ser Leu Met Gln Ala
65 70 75 80
Asp Met Glu Leu Gln Leu Asn Gly Thr Tyr Asp Cys Leu Asp Ala Val
85 90 95
Ile Phe Ser Val Pro Cys Asp Thr Leu Arg Cys Met Ser Gln Lys Trp
100 105 110
His Gly Lys Ala Pro Val Ile Val Phe Thr Gln Pro Gln Asn Arg Lys
115 120 125
Ile Arg Pro Ala Val Asp Phe Leu Lys Ala Glu Tyr Glu His Val Arg
130 135 140
Thr Glu Leu Glu Arg Ile Leu Asn Val Lys Ile Ser Asp Leu Ala Ile
145 150 155 160
Gln Glu Ala Ile Lys Val Tyr Asn Glu Asn Arg Gln Val Met Arg Glu
165 170 175
Phe Cys Asp Val Ala Ala Gln Tyr Pro Gln Ile Phe Thr Pro Val Lys
180 185 190
Arg His Asp Val Ile Lys Ala Arg Trp Phe Met Asp Lys Ala Glu His
195 200 205
Thr Ala Leu Val Arg Glu Leu Ile Asp Ala Val Lys Lys Glu Pro Val
210 215 220
Gln Pro Trp Asn Gly Lys Lys Val Ile Leu Ser Gly Ile Met Ala Glu
225 230 235 240
Pro Asp Glu Phe Leu Asp Ile Phe Ser Glu Phe Asn Ile Ala Val Val
245 250 255
Ala Asp Asp Leu Ala Gln Glu Ser Arg Gln Phe Arg Thr Asp Val Pro
260 265 270
Ser Gly Ile Asp Pro Leu Glu Gln Leu Ala Gln Gln Trp Gln Asp Phe
275 280 285
Asp Gly Cys Pro Leu Ala Leu Asn Glu Asp Lys Pro Arg Gly Gln Met
290 295 300
Leu Ile Asp Met Thr Lys Lys Tyr Asn Ala Asp Ala Val Val Ile Cys
305 310 315 320
Met Met Arg Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Ile Tyr Lys
325 330 335
Pro Glu Phe Glu Ala Ala Gly Val Arg Tyr Thr Val Leu Asp Leu Asp
340 345 350
Ile Glu Ser Pro Ser Leu Glu Gln Leu Arg Thr Arg Ile Gln Ala Phe
355 360 365
Ser Glu Ile Leu
370
<210> 98
<211> 258
<212> PRT
<213> Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl)
<400> 98
Met Ser Glu Glu Ser Leu Val Leu Ser Thr Ile Glu Gly Pro Ile Ala
1 5 10 15
Ile Leu Thr Leu Asn Arg Pro Gln Ala Leu Asn Ala Leu Ser Pro Ala
20 25 30
Leu Ile Asp Asp Leu Ile Arg His Leu Glu Ala Cys Asp Ala Asp Asp
35 40 45
Thr Ile Arg Val Ile Ile Ile Thr Gly Ala Gly Arg Ala Phe Ala Ala
50 55 60
Gly Ala Asp Ile Lys Ala Met Ala Asn Ala Thr Pro Ile Asp Met Leu
65 70 75 80
Thr Ser Gly Met Ile Ala Arg Trp Ala Arg Ile Ala Ala Val Arg Lys
85 90 95
Pro Val Ile Ala Ala Val Asn Gly Tyr Ala Leu Gly Gly Gly Cys Glu
100 105 110
Leu Ala Met Met Cys Asp Ile Ile Ile Ala Ser Glu Asn Ala Gln Phe
115 120 125
Gly Gln Pro Glu Ile Asn Leu Gly Ile Ile Pro Gly Ala Gly Gly Thr
130 135 140
Gln Arg Leu Thr Arg Ala Leu Gly Pro Tyr Arg Ala Met Glu Leu Ile
145 150 155 160
Leu Thr Gly Ala Thr Ile Ser Ala Gln Glu Ala Leu Ala His Gly Leu
165 170 175
Val Cys Arg Val Cys Pro Pro Glu Ser Leu Leu Asp Glu Ala Arg Arg
180 185 190
Ile Ala Gln Thr Ile Ala Thr Lys Ser Pro Leu Ala Val Gln Leu Ala
195 200 205
Lys Glu Ala Val Arg Met Ala Ala Glu Thr Thr Val Arg Glu Gly Leu
210 215 220
Ala Ile Glu Leu Arg Asn Phe Tyr Leu Leu Phe Ala Ser Ala Asp Gln
225 230 235 240
Lys Glu Gly Met Gln Ala Phe Ile Glu Lys Arg Ala Pro Asn Phe Ser
245 250 255
Gly Arg
<210> 99
<211> 900
<212> DNA
<213> Dictyostelium discoideum (Slime mold)
<400> 99
atgattaata gattattttc aattaataat attaaaaatg gatcaaaatt ttttagttca 60
tcaacaacag ttgaaactaa acaaccatta gttttattag aaaaacattt agtaaatgga 120
aaatatacag gtattcaaat tgttaaatta aataaaccaa aacaattgaa tgcattaaca 180
tttgaaatgg gagttgatta taagaaggtg gtggatacat tagcagaaga taaagatttg 240
aaatgtgttg tattgacagg tgaaggtaag gcattttcgg caggtggtga tttagatttc 300
ttaattgaaa gaactaaaga cacaccagaa aacaatcaaa gaattatgga aagattctat 360
agaacatttt tatatattcg ttcattacca gtaccaatca tttctgcaat caatggtgca 420
gcaattggtg caggtttctg tttagcttta gcaactgata ttcgtgtcgt tagtaataaa 480
gcaccagtgg gtttaacatt caccaaatta ggtattcatc caggtatggg tgtaactcat 540
tcaattacaa atatagttgg tcaagatgtt gcatcctata tgttattatc aagtgatatt 600
atcaaaggtg atgaagctca aagattaggt ttagttttaa aatcggttga atctgatcaa 660
gttttaccaa ctgctttaaa tctcgctgaa acaatctcaa aaaattcaac tatcgctgta 720
aactctacaa caaaaacttt acgtaataaa tataattcag atttagataa aagtttaact 780
cgtgaagctg atgctcaaag tcaatgttgg gcttcaaaag atatagttga aggtatttta 840
gcaattagag aaagtagaga tccaaaacat aattatttat tatttgatga tcaaaaataa 900
900
<210> 100
<211> 786
<212> DNA
<213> Clostridium acetobutylicum
<400> 100
atggaactaa acaatgtcat ccttgaaaag gaaggtaaag ttgctgtagt taccattaac 60
agacctaaag cattaaatgc gttaaatagt gatacactaa aagaaatgga ttatgttata 120
ggtgaaattg aaaatgatag cgaagtactt gcagtaattt taactggagc aggagaaaaa 180
tcatttgtag caggagcaga tatttctgag atgaaggaaa tgaataccat tgaaggtaga 240
aaattcggga tacttggaaa taaagtgttt agaagattag aacttcttga aaagcctgta 300
atagcagctg ttaatggttt tgctttagga ggcggatgcg aaatagctat gtcttgtgat 360
ataagaatag cttcaagcaa cgcaagattt ggtcaaccag aagtaggtct cggaataaca 420
cctggttttg gtggtacaca aagactttca agattagttg gaatgggcat ggcaaagcag 480
cttatattta ctgcacaaaa tataaaggca gatgaagcat taagaatcgg acttgtaaat 540
aaggtagtag aacctagtga attaatgaat acagcaaaag aaattgcaaa caaaattgtg 600
agcaatgctc cagtagctgt taagttaagc aaacaggcta ttaatagagg aatgcagtgt 660
gatattgata ctgctttagc atttgaatca gaagcatttg gagaatgctt ttcaacagag 720
gatcaaaagg atgcaatgac agctttcata gagaaaagaa aaattgaagg cttcaaaaat 780
agatag 786
<210> 101
<211> 468
<212> DNA
<213> Clostridium difficile
<400> 101
aatagtaaaa aagtagtgat agctgctgta aacggatttg ctttaggtgg atgtgaactt 60
gcaatggcat gtgatataag aattgcatct gctaaagcta aatttggtca gccagaagta 120
actcttggaa taactccagg atatggagga actcaaaggc ttacaagatt ggttggaatg 180
gcaaaagcaa aagaattaat ctttacaggt caagttataa aagctgatga agctgaaaaa 240
atagggctag taaatagagt cgttgagcca gacattttaa tagaagaagt tgagaaatta 300
gctaagataa tagctaaaaa tgctcagctt gcagttagat actctaaaga agcaatacaa 360
cttggtgctc aaactgatat aaatactgga atagatatag aatctaattt atttggtctt 420
tgtttttcaa ctaaagacca aaaagaagga attgtcagct ttcgttga 468
<210> 102
<211> 777
<212> DNA
<213> Clostridium pasteurianum
<400> 102
atgggaaata ttatctttga agaagaagat ggaatagaaa aagttacaat taacagacct 60
aaagctctta atgcattaaa tagtgaaaca ttaaaagaac ttggtacagt aataaatgac 120
atatctgtaa acgatggaat aaaagctgta ataataacag gttcgggatc aaaagctttt 180
gtagctggtg cagatatagc tgaaatgagt actctaaatt caatagaggc aacaaatttt 240
tcaagacttg cccaaaatgt attttcacaa atagaaaatc tacctaaatt agtagtagca 300
gcagttaacg gttttgctct tggaggagga tgtgagcttg caatggcttg tgatgtaagg 360
tttgcttcaa aaaaagctaa atttggtcaa ccagaagtta atttaggaat attgccaagt 420
ttcggaggaa ctcaacggct tccaaaattg gttggaaagg gaatagcaaa agaattgata 480
ttttctacag atatgattac tgccgatgaa gcttatcgta taggacttgc taataaagtc 540
tatgaacctg aggaattatt agtaaaatca caggagtttg ctgaaaaggt aatgactaaa 600
tctccatggg gtgttaaatt agcaaaagca tgtataaata atggattaga tgtagatttg 660
gaagcaggac ttaaatatga agcaaattca tttggtctgt gtttttcaac ggaagatcaa 720
aaggaaggta tgaaagcatt tttagaaaaa agaaaagcag acttcaaagg actttaa 777
<210> 103
<211> 789
<212> DNA
<213> Clostridium pasteurianum
<400> 103
atggatttta ataatattat ccttgaaaaa gaggaaaaaa ttgccgtagt tacaattaat 60
agacctaaag ctcttaatgc tttgaacagt gaaacgttaa ctgagcttga ttctgtaatt 120
gatgaaattg acaaagataa tgaaatttta gcagtggtat taacgggagc gggaaaatcc 180
ttcgtagctg gagccgatat atcagaaatg aaagacatga atgtagtaga aggaagaaaa 240
tttggaatac taggtaataa ggtgttcaga aaacttgaaa atttagaaaa gccagtaata 300
gcagccctta atggatttac attgggtggt ggttgtgaaa ttgctatgtc ttgcgatata 360
agaatagctt ctactaaggc aaaatttgga cagccagagg tacagcttgg aataactcca 420
ggttttggcg gtactcaaag attagctaga ttaataggcc caggagctgc aaaggaactt 480
atatatactg gaaaaattat aaatgctgaa gaggcctata gattaggact tgttaataga 540
gttatagaac cagaaacttt attagatgaa gcaaaacaat tggcaaatac tatagcagcc 600
aatgcaccta tagctgttaa gttggctaaa tcagcaataa atagaggaat tcaaactgat 660
attgatacag gtgtgtcaat tgaatcagaa gtatttggag cttgtttctc tacagaagat 720
caaaaagaag gtatgaatac attcttgaat gataaaaaat atttaactgg taattttaag 780
aataaataa 789
<210> 104
<211> 783
<212> DNA
<213> Megasphaera elsdenii
<400> 104
atggattacc agaacattat ttttgctgta gaagacggta ttgcaacgat tacgatcaat 60
cgcccgaagg ctctgaacgc tttgaaccag gctacggtca gcgaattgaa agacgtcgtt 120
gaaaagattg cagctgataa agctatcaaa gtcgtcatca tcaccggtgc aggcgctaaa 180
tccttcgtcg ctggcgctga catcaaagaa atggcttcca agaacgctgc tgaaggccgc 240
gaatggggcc agttcggtca gaacgtcttc acggaaatcg aaaacctgcc gcagcctgtc 300
atcgcagcta tcaacggctt cgctctcggc ggcggctgcg aactctcctg cgcttgcgat 360
atccgctatg cagctgaaaa cgctaaattc ggccagccgg aagtcggctt gggcatcact 420
ccgggctttg gcggcacgca gcgcctgacc cgtgtcgtag gccgcggcca cgcgaaagaa 480
ctcatctaca cgggcggcat gatcgacgct gaaaaagcaa aagctatcgg cttggtcaat 540
gaagtcttcc cgcaggaaga actgatgccg gctgctgtta aattggctaa gaagatcgct 600
aagaacgctc ctattgcagt acagctctcc aaagctgcca tcaaccgcgg catcaactgc 660
gacgtcgtaa ccggtatcgc ttatgaagct gaagtcttcg gcctctgctt ctccacggct 720
gaccagaagg aaggcatggc tgctttctgc gaaaaacgca aagcaacgtt tgaaggtaaa 780
taa 783
<210> 105
<211> 780
<212> DNA
<213> Metallosphaera sedula
<400> 105
atggaatttg aaacaataga aactaaaaaa gaaggaaact tgttctggat tacgttaaat 60
agacccgata aactaaacgc actaaacgct aaattacttg aggagttaga tagggcagtc 120
tctcaggcag agtctgaccc agagattagg gttatcatca ttacagggaa aggaaaggcc 180
ttctgcgcag gggctgacat aacccagttt aaccagttaa ccccagcaga agcctggaaa 240
ttctctaaga aaggaagaga gatcatggac aagatagagg cactgagcaa acccaccatt 300
gccatgatca atggatatgc ccttgggggt ggactagagc tagccttagc ctgtgatata 360
aggatcgcag cggaggaggc ccaactaggc cttccagaga taaacctagg gatatatccg 420
gggtatgggg ggactcagag gttaaccaga gttataggaa agggaagagc cctggagatg 480
atgatgacgg gcgatcgtat tcctggtaag gatgctgaga aatatggtct cgtgaatagg 540
gttgtccccc tagctaactt ggagcaagag acaaggaagc tggcagaaaa gatagccaag 600
aagtctccta tctctctcgc cttaatcaag gaagttgtaa acaggggact agactctccc 660
ctactgtcag gtctagcgtt ggaaagcgta ggatggggag tcgtgttttc tacggaggac 720
aagaaggagg gggtaagtgc cttcctggag aagagagagc ctacgtttaa gggaaaatag 780
780
<210> 106
<211> 779
<212> DNA
<213> Clostridicum kluyvery
<400> 106
atggaattta aaaatatcat tcttgaaaag gatggaaatg tggcttcaat aacgttgaat 60
agacctaagg cattaaatgc attaaatgca gcaactttaa aagagataga tgccgcaata 120
aacgacattg ctgaagatga taacgtatat gctgtgataa ttactgggtc aggtaaagct 180
tttgtagcag gagcagatat agctgagatg aaagatctta ctgcagttga gggaagaaag 240
ttttcagttc ttggcaataa aatatttaga aaattagaaa atttagaaaa accagttata 300
gcagctataa atggatttgc actgggtggt ggctgtgaat tgtcattgtc ttgcgatata 360
agaatagctt catcaaaggc taagtttggt caaccagagg ttggtcttgg aattactcca 420
gggtttggag gtactcaaag acttgcaaga gcaataggcg ttggtatggc taaggaactt 480
atatataccg gaaaagtaat taatgctgaa gaggcattaa gaataggttt ggtaaataaa 540
gtagttgagc cagataaatt attggaagaa gctaaagctt tagtagatgc tattattgtt 600
aatgcaccta tagctgttag aatgtgtaag gctgctataa atcaaggact tcagtgtgat 660
atagatacag gtgtagctta tgaagcagaa gtatttgggg aatgttttgc tacagaagat 720
agagtagaag gaatgacagc atttgtagaa aaaagagaca aggcttttaa aaataagta 779
<210> 107
<211> 1509
<212> DNA
<213> Sulfolobus tokodaii
<400> 107
atggcaatta gaactggaga gcaatattta gattctataa aaattagaaa taaggctgaa 60
atttacgtaa tgggaaaaga agtaaaggat gtaaccactc atcccttctt gaaaccttct 120
gtaatggcat ttaaggcaac atttgatgct gcttgggaag aggacacaaa agaattagcc 180
agagcatgga gtcctttcat aaatgaagaa gtgaatagat ttaatcacat acacaggtca 240
ccagaagact tagctgctaa agtgaaatta ctgagaaaat taagccataa gaccggtgca 300
tgtttccaaa gatgtgtagg atgggacgct ctgaacactt tgtggattat gacgaatata 360
atggctcaaa aaggtaaaaa agaatataag gatagatttg tcgaatactt aagttacgtc 420
caaaagaagg atttagcatt agctggtgct atgacagatg caaaaggtgt aagaacatta 480
aaaccgcatc aacaaccaaa taagaacgct tatgttagaa ttgaggaagt taccaaagac 540
ggtatttatg tttctggtgc aaaggcaaat attactggtg tagctgcaac agaagaaatt 600
gtggttttac ctactagggc tatggggcca gaagataaag attatgctgt tgcattttca 660
ataccgacag atactgaggg tataaaaatt atagttggta gacaattaaa tgatgctaga 720
agattagaag gtggtgacat agatgcttta ccgtacttct ataaccacga gggtttagta 780
atctttgacc atgtttttgt accaatggat agagtattct taatgggaga atacgagttt 840
acttcacaat tagttgaagt attctcagca tatcatagac aaggatatgg tggttgcaag 900
gctggtttag gagatgtaat tattggtgca tcaatgaatt tagcaaaaca attaggagta 960
gaaaaagctt cacatgtaca agaaaaacta acggaaatga tattcttaac tgagaccatg 1020
tattctgcag gaattgcagc tagtttaaat gcagttaagg tctgcgataa ttgttggtgg 1080
gttaatccta tgcacgctaa tgttacaaaa catttagtag ctagatttcc agcccagatt 1140
tctcagttat ctatcgatat tgcaggtgga ataataggta ctgcaccaag tgagtgggat 1200
ctcaagaatc ctaaattaag agaatatatt gccaaatact tacaaggtgt tgagggttat 1260
acagctgaag atagattaag aatggttaga ttactggaaa acgttagtct gggtgttgca 1320
ttccaaattg aatctgtaca cggtgcagga agtccagcag cacaaagaat aatgtttagt 1380
agactttatg acttaaacta tgctgaggaa gtcgcaaaga ggttagctgg gaagaagact 1440
gatttacagt ggaaacctaa agcagagcct tggagagaaa gtgagacaga aaaattagta 1500
aaaagttaa 1509
<210> 108
<211> 1452
<212> DNA
<213> Geobacter metallireducens
<400> 108
atggcactaa gagatgggaa ttcctaccgg gaaagccttc gggcgctcaa tatcaaagtc 60
tatgcctttg gagagaagat tgacagcata gtagatcacc cattgttcca gccccatatc 120
aatgcggctg cattgacgtt cgacttggcc catgatccga ccacggaagc gctcgtcaca 180
gccacctcac acctgacggg gagtaaaatc agccgcttca cccatatcca ccagagcacc 240
gacgatctca taaaaaaggt gaagatgttg cggcttattg cagggaagac gggaagttgc 300
taccagcgct gtgtggggtg ggatgccctg aacgctaact atacggtaac ctatgagatg 360
gaccaggagc ttggtaccga ctatcaccag cgttttaggc gttacctcga atatatacag 420
gacaatgacc tgatggtggc gggagcaatg accgatccca agggggacag ggggctgcct 480
ccggcaaaac agaaagaccc ggacatgttc gtgcacgtgg tggcaaagaa tgacaagggg 540
atagtcattc gtggggcaaa ggttcaccag accggaattg tcaattccca tgaaatgctg 600
attatgccaa ccatggccat gggggaggag gacggcgact atgcggttgc ctgtgctctc 660
cccacggatt cccccggtgt catccatatc tttggtcgtc aaaccaacga tacacgccgt 720
ctggaaaagg gagaccttga tcagggtaat gctgagtatg gaactgtcgg aggcgaggct 780
ttgaccatac ttgaagatgt cttcgtcccg tgggaacgcg tcttcatgtg cggagagtac 840
aagtatgcgg ggctgctggt tgagcgtttc gcgagctatc atcgacagaa ctatggtgga 900
tgcaaggcag gcgtgagcga tgtgatcatc ggcgcaacta ccgctatggc agagtacaac 960
ggagcagcca aggcttccca cgtgcgtgac aagatcgtgg agatggtcca cctcaccgag 1020
accctttatt gcggttccat cgcctgctcc tgtgagggtg ctcccacgcc gtcaggggcc 1080
tatttcgtca atcccctgct ggccaatacg gttaagcaga acgtgacccg tttcatctat 1140
gagattgcac gcctttccca cgatatttcc ggtggctgca tggcaaccat gccttcggag 1200
aaggatctgc accacgatga gatcggcaaa tatgtagaga agtatttccg gggggtggac 1260
gaagctccca ctgaagagcg catgcggatg gcccggctcg ttgaaaatat gacgggcggc 1320
acggctttgg tggaaagcat gcatggtgcc ggctctcccc aggcgcagag agtcatgatc 1380
ctccgccagg caaatctcgg ccataaggta aagcttgcca agaaactggc cggcataaag 1440
gaagaaaaat ag 1452
<210> 109
<211> 1392
<212> DNA
<213> Sulfolobus solfataricus
<400> 109
atgagatcaa aagaagattt cctaaagtcc ttaaaagatg gaagaaattt gtattatagg 60
gggaagttag tagaagatat aacaacacat cagatcttaa agacagccgc attgcacgca 120
gctaagttat atgaatacgc tgatagagtc tatgaggata ataaaatggg aaaaatgagc 180
aagttcttta aggtaccttg gacatctcaa gatttgctag atagacataa actaatttac 240
gatttaacga tgtattgtaa tggggtattt aacatttcac aagcaatagg aagtgatgcg 300
atctttgccc ttatgatcac ggcaaaacaa gttgatagaa aatacggaac tgattactca 360
aaacgtgttg aaaaatattt tgagagagtt gctaaagaag atttaacgtt agccactgcc 420
cagactgacg ttaagggaga tcgaagtaag aggccttctg aacaagttga tccagatatg 480
tatgttagag tagttgatgt gaaaagcgat ggaatagttg ttagaggagc aaaggctcat 540
acaactcaat ctgcggtatc tgatgagatt attgtcatac caaccagagt aatgagggat 600
agcgataaag attacgcagt agcctttgcg gttccagcta atactaaagg tttgaagatg 660
tatattagac caattgatga aattgagggc aattcctcct cagtactcag tagaaaagat 720
tatgagctag aaacattaac cgtcttcaac gacgttttcg ttccttggga tagggtattt 780
ttatttaagg aatacgacta cgctggaaca ttggctatgc tatttgcaac cttccatagg 840
tttactgcat tatcgtatag gtcagcgacc atgaatctat atttgggagc atctaaagtg 900
gcatctcaag taaatggcat tgagaatgaa aagcatgtga gagatgatat agttgatata 960
attctctaca aggaaattat gaggagtagc gcgatagctg cggctgtgta tccagtaaac 1020
atggagggta tagctgtgcc caacccgctt tttactaatg ttggtaaatt atactccaat 1080
atgcatttcc atgatgttgt aagagattta attgacattg ctggggggat aatagctact 1140
atgccctctc aagaagattt ggaaagtgat gaaggaaaga atattgttaa atatttaagg 1200
ggctcagttg atggagagga aagagcaaaa gtgttaaaac tagctaagga attaggggct 1260
agtacgttta ctggctattt gctaactggt atgatacatg cggaaggttc tatggaagct 1320
agcaaaatag agctattcag aagttataat tttaaggagg ccgagaactt agttaaaagg 1380
gtattaagct ag 1392
<210> 110
<211> 1440
<212> DNA
<213> Syntrophobacter fumaroxidans
<400> 110
atgggactca aaacgaaggc ggaatatata gaatccttgc gaggcatgaa gccgacggtc 60
tacatgttcg gtgagaagat cgaaagcgtt gtggacaatc cacgcctgcg agcgggcatc 120
gaggcgacgg gggcgacgta cgaactggca gagacggagg agtatcgccc tctcattgtg 180
actgaaagtc ccctcattca cgaacccgtc aaccggtata cgttgccccc gtcgtccatc 240
gcggacctcg tcgccagggt gaagatcaat cgtctcatgg gcactcgtgt cgggacctgc 300
tttcaacggt gcacggggct ggactgcctg tccgcccttt ccatcgtgac ctacgacatc 360
gacgccaagc attccacccc ttacttcaaa cggttcatcg agtttctgaa gcatgttcag 420
aaaaacgacc tgacctgcaa cgccggcgtg accgacgtca agggcgaccg ttccctggcc 480
ccccacgagc aggaagacaa ggacatgtac gtgagggtcg tggaacgcaa tgcggacggc 540
atcgtcgtga ggggcgccaa ggcgcaccag accggttccc tctcctcgca cgaaatcatc 600
gtcctgccga cgcgtgccct gcgaaagggc gacgaggact acgcgctcgc ttttgccatc 660
cccaacgaca ctcccggcct gattcacgtc gtgggccgat cgagcctcga cacccgccag 720
ctggacggct gcgacctggg caaccttcac tattccaagt actgcccgac cgtgatcttc 780
aaggacgtgt tcgttccctg ggagcgggtc ttcatgtgcg gcgaggtgga attcgccgtg 840
gagatggtga accgcttttc ggcttatcac cgccagagcc acggcggctg caagtcgggc 900
aagatcgact gcatggtcgg agcggccctc accatgatgg actacaacgg gacggagaag 960
gccgggcatc tcaagcagaa ggccatcgag atggtccacc gggcggaaac cctctacggc 1020
tgcagcctgg ccgcgtccta cgagggcaaa aaagaacctt ccggaaccta cttcatcgac 1080
acggtgctgg ccaatgcgtc caagatccac gaaggcaagg aaatgagcga ggccggccgc 1140
ctgctggtgg acatcgccgg aggcttcgtg gccgatctgc cttcggatcg cgacctggcc 1200
attcccgaag tcggggaact gctgaaaaaa tacctgaagg gggtggcgtc ggtgccggtg 1260
gaagaccgcg tcaaaatgta ccggctgatc gaaaagctcg tcatggaaag cgccgatacg 1320
atttcggaca tccatggagg cggttctccc gaggcccaca ggatcacgat cctgcgggaa 1380
agcaacctca aggccaagaa ggacgcggcc aagcggttgg cgggaatcga atcgaagtag 1440
1440
<210> 111
<211> 1461
<212> DNA
<213> Porphyromonas gingivalis
<400> 111
atgatgacta gcgaacagta cgtagaaagt cttcggaaac ttaatctgaa ggtttacttc 60
atgggtgaaa ggatcgaaaa ccctgtagat catcccatga ttcgtccctc aatgaattca 120
gtagctatga cttataagct tgctgagatg gacgaataca agcatttaat gacagcaact 180
tcaaacttga ctggtaagca agtgaatcgt ttctgccatc tacatcagag cacagaggat 240
ctgaaagaca aagtgaagat gcagcgtctc atgggacaaa aaacagcttc atgcttccag 300
cgttgtgtgg gaatggatgc attcaatgcc atctattcta ctacttacga aatggatcaa 360
gctctgggta ccacttatca caagcgtttc atcgagtaca tgaaatatgt acaagacaac 420
gacttggtcg tagatggagc catgacagac cccaaagggg atcgcggttt atctccctca 480
gaacaagccg atccggatct ttatctgcac attgttgaag ttcgtgaaga tgggatcgtc 540
gtttccggtg caaaggcaca ccaaaccgga gcagtcaatt cgcacgagca tctgatcatg 600
cctacgatcg ctatgcgcga agctgatgct gactatgccg tttcttttgc cgttcccagt 660
gatgcagagg gcgttattat gatctatggc cgccagtcat gcgacactcg caaaatggaa 720
gaaggggcag acattgacct cggcaactct gaattcggcg gacatgaagc tcttgttgta 780
ttcgaccgcg tattcgtgcc caatgaccgc gtgttcatgt gcaaagaata ccagtttgca 840
ggtatgatgg tagaacgttt cgccggatac caccgtcagt cttatggagg atgtaaagta 900
ggtgttggtg atgtacttat cggtgcagct gctctcgcag cagactacaa tggagttcct 960
aaggcatctc acattaagga taaactcatt gagatgatcc acctgaatga aaccctttat 1020
gcttgcggta ttgcatgctc ttcagaggga actcagatga aagccggcaa ctatatgatc 1080
gatttgctgt tagctaatgt ttgtaagcaa aatatcaccc gccttcctta tgaaatagct 1140
cgcttggcag aagatattgc aggaggtttg atggtaacca tgccttctca acaagacttc 1200
cgccatccgg aaataggccc gatcgtaaag aaatatcttg caggggcaac aggcaaatcg 1260
acagaaaacc gtatgcgtgt tctgcgtttg atagagaata tcacgctggg aacagctgcc 1320
gtcggttatc gaaccgagtc tatgcacgga gccggatctc ctcaagctca gagaatcatg 1380
atcgctcgtc agggagatct tgagggcaag aaaaagcttg cacgggcgat tgctcatatc 1440
gacgaatcac tcgataagta a 1461
<210> 112
<211> 1590
<212> DNA
<213> Polynucleobacter necessarius subsp. Asymbioticus
<400> 112
atgagtcaaa gcacctccca gttcatgaat agcaaagact atcaagagtc attgcgctca 60
ctaaagccaa ctgtctatgt cgatggtcga ttgatcgaat ccgtcgccga tgagccttct 120
cttcgccctg gagtccaagc cttaggagtg acttatgaca tggtccatga cccagcgcta 180
gcaccgctca tgttggctga ctcgaatggc actcctgtac caagaatgct gcacattaat 240
cagtcttctg gagatctctt aaataaatta gaagcggtac gtgtactctg ccaagaaact 300
ggatgtgccc aacgctattt agcccatgat gcgttaaatg cgattgcaca agtttctgcg 360
cgcattgatg atgccaaagg aagtaatgag catagtgcta aattttctga gtatctatcg 420
catgtacaaa cgaaggactt ggcattaggc attgccatga cagatgcaaa aggagatcgc 480
tcccgcagac ctcatgagca agaaaatcca gatacttacg tacatatcgt ttctcaagat 540
gctaaagggg tcgtgatctc gggtacaaaa gcgattgtga ctggcgcccc ttacatgcat 600
gaattcttag tcatgccagg tcgcaatatg actaaagagg atgcagcctt tgcgatttgc 660
tgtgctgtcc ctgtggatgc caaaggtatt acgattgtgg cacgcccagc gggacgccca 720
ggcgacaagg tcgagcatgg taaaccgata ttttctagta aatatggtca atcgactggg 780
gtagtgatat tcgataaagt attcgttccc tgggatcgtg ttttttatgc tggcgaatgg 840
gaacactcta gcgtgctgac ttataactac gccacccatc atcgtcatag ctgcatcgcg 900
gcgcgagcag gctttggaga tctgttaatt ggtgctggcg ctttaatgtg cgaagcgaac 960
ggattggatc cagcaaccaa atctaattta cgtgatccga tggttgaact cattaagatc 1020
actgaaggat tttatgcttg cggtgtggct gctagcgtct atggaacgca agatccgtac 1080
agtaaatcat ttatgcctga gccggtattt tctaatatcg gaaaactctt attagcaacg 1140
cagatttatg acatgcatcg cttggcacat gaagtatcgg gaggattaat cgtagcgttg 1200
ccaggaccag acgaagatca caacccagca actgcagcca ctttggcaga ggtgttacga 1260
gccaatccag ccgtccctta tgacaagcga attgaagttg cacggtttat tgaagatctc 1320
acagcgtctt atcaaggcgg ttggtattcc gtcattagcc tacatggtgg cggctctcca 1380
gcagcaatga agcaagaaat ctatcgtcag taccctattg gcaataaagt agagctagtg 1440
gaacgtttat tagatcgcgg agtgctgact agtagcgaag agcgggcgat tacgaaaaat 1500
aaacaacctg ggcgctgctg cgatcaaggc tgtagcgcgc caggacaagc agtgatggta 1560
cctttgccag agcctggcag aagaacttaa 1590
<210> 113
<211> 777
<212> DNA
<213> Gordonia terrae C-6
<400> 113
gtgaccgaac accagaccat cgtcgtcgag accagcggcc gggtgggcat catcaccctc 60
aaccgcccga aagcgctgaa cgcgctcaac accgagttga tgaacgaagt ggtcggcgcc 120
gtcaaggagt tcgacgtcga ccaggggatc ggcgccatcg tgatcaccgg ttcggagaag 180
gcgttcgccg cgggcgccga catcaaggag atgtcatcga agtcctacgc ggatgtggtg 240
aacgagcagt tcttcggcgc ctgggatgag ctgtcgcggg cgcgtacgcc gatcatcgcc 300
gcagtgaccg gctacgccct cggcggcggc tgcgaactcg cgatgctgtg cgacaccatc 360
atcgccggcg acaacgccgt cttcggtcag cccgagatca acctcggcgt catccccggc 420
atcggtggtt cgcagcgcct cacccgcgcc gtcggcaagg ccaaggcgat ggacatggtg 480
ctcaccggcc ggcagatgaa ggtcgacgag gccgagcgtc tgggcctggt ctcgcgggtg 540
gtgcccaagg aggactgccg cgccgccgcg atcgaagtcg ccgagataat cgcctcgaag 600
tcgctgatcg ccgccgcggc cgccaaggac gcggtcaacc gtgccttcga gtcgagcctg 660
gtggagggtg tccgcgccga gcgcgcgctg ttctactcga cgttcgcgac cgacgaccag 720
accgagggca tggccgcctt cgtcgagaag cgggacccga acttcaccca ccgctga 777
<210> 114
<211> 777
<212> DNA
<213> Halalkalicoccus jeotgali
<400> 114
atggcagaca gagtactcat cgaacgagag aatgacatag cgacgatcat cgttaatcgg 60
cctgagaagc gtaatgcgat ggatatcccg acgcgaaaag ccctctatgc cgccttcgaa 120
gaggttagcg aggatgacga tgtgcgggca atcgtgctcc gcggagcagg agatgggtcg 180
tttatcgccg gtggcgatat tgattctttc gccgacttcg accacatgga cggcatggag 240
tacagcgaga agtacgccca agggctgtac aactatgttg cggaccgcca caaaccaacc 300
atcgccgcgg ttgacggcta cgctctcggt ggaggcaccg aaatcgccct cgcttgcgac 360
attcgcctcg ccacggacga cgcgaagttc ggcctgcccg aagtcggcat cggcgtcatc 420
ccagccggtg gtggaacaca gcgactcgtt caagtcgtcg gagccgggct tgcaagcgaa 480
cttatcctca ctggccgcat tatcagcgcc gacgaggcaa agagaattgg tcttgcaaac 540
catgtctacg ccgccgagga attcgataat gaagtccgag ccatggccga agatcttgcc 600
tcgaaggcgc ctgtcgccca gcgacttgca aaagaatcca tccgacgtag ccttgatatc 660
gacgccggcc ttgaatacga gcgactggcc ggagcgtttc tgttcggcac cgacgaccag 720
aaagagggtg caaacgcctt ccttgaggac cgagagccga agtaccgaaa ccggtaa 777
<210> 115
<211> 774
<212> DNA
<213> Carboxydothermus hydrogenoformans
<400> 115
gtggaatttg aaaaaattaa atttgaggtt acggacggtt atgccgttat ttacctaaac 60
aacccgccgg taaatgctct tggccagaaa gttttaaaag atttacaaaa agctttgcag 120
gaaattgaga aaaatcccga gattcgggcg gtaataatta gcggggaagg tagcaaggtt 180
ttctgtgccg gggcagatat cacggaattt gctgaccggg ctaaagggat tttaccggaa 240
gtggaaggaa gtgttctttt ccggcaaatt gagcttttcc ccaagccggt gattgctgcg 300
ctgaacggta gctcctacgg cggaggaacc gaattagcga taagctgtca cctgcgcatt 360
ttagcagatg atgcttccat ggctttgccc gaagtaaaac tgggcattat ccctggctgg 420
ggaggtaccc agaggttacc ccggttaatt ggtaaaacca gagccctgga agcaatgctt 480
accggagagc caataacggc agaagaagcc ttaagctacg gtctggtaaa caaagtcgta 540
cccaaagacc aggtactaac agaagcccgg gcgctggcag ctaagcttgc caaaggggcg 600
cccatcgcta tgcgggaaat tttaaaggcg gtaactttag ggctggatac ttcaatagaa 660
gaaggtttaa aaattgagaa agaaggttcc aaagtggcgt ttagcagtga agatgcggtg 720
gagggaagaa ctgctttctt tgaaaaacgg ccgccgaatt ttaaaggccg gtaa 774
<210> 116
<211> 774
<212> DNA
<213> Thermomicrobium roseum
<400> 116
atgagcgtgc gtgtcgagcg ggagggggcg atcaccctcg tcacggtcga gcgcccggaa 60
cgactgaacg cgctcgatac cgcgacgttg cgtgccttac tcgcggcagt gcaggaactg 120
gcaacggagg aggcgatcgc tgtcgtcgtc ctcaccgggg caggcgatcg cgcgttcatc 180
gccggagccg atatcagcga gatggtagag aagtcgccag ccgaggcgct cgccttcgcc 240
gagttgggac acgccgtttg ccgggcgatc gaggaagcgc cgcaaccgta catcgcagcg 300
gtcaatggct acgcgctagg aggcggctgc gagatcgcgc tggcgtgcga tatccgcctc 360
gccagcgagc gcgccgtctt cgcccagccg gaagtaacgc tgggtattcc accaggctgg 420
ggcggatcgc aacggctgcc gcgcgtcgtt cctcctggta tcgcgcgcga gttgctctat 480
acggggcgcc gcgtcgatgc gcaggaagca ctgcggatcg ggctcgtcaa tgccgtctat 540
ccggctgacc aactcctcga gcgagctcgg gaactggcga accggatcgc ggccaacggg 600
ccactcgcgg tccgcttgac caaggcggcg gttcgcttcg gtctcgagca ggggctggaa 660
gctggactga cctacgagcg gcaggtgttc gcgtacgcgt tcaccaccga ggatcagcgg 720
gaggggatgc gggcatttct ggaaaagcgt cgtccggctt ttcgcgggcg ctga 774
<210> 117
<211> 825
<212> DNA
<213> Methylobacterium extorquens
<400> 117
atgaacgctg acgccgagac cgcctcgacc gacgaactgc tcttcgcggt ggatgcggcg 60
ggcatcgccc gcatcaccct caaccggccg aaggcgcgca acgcgctgac cttcgcgatg 120
tatcgcgggc tggtggagtt gtgcgagcgg atcgaggcgg accacgcgat caaggcggtg 180
atcatcaccg gcgccgggga caaggcgttc gcggcgggta ccgacatcgc ccagttccgt 240
agcttcagca aaccggaaga cgcgatcggc tacgagcgct tcatggaccg ggtgctcggc 300
ggcctggagc gcctgcgggt gccgaccatc gcggcggtcg ccggagcctg caccgggggc 360
ggtgcagcga tcgctgcggc ctgcgacatg cgcatcgcca gccgcgacgc ccgcttcggc 420
atccccatcg cccgcacgct cggcaattgc ctctcgcaga acaccctgag gcggctggcg 480
aacctcattg gggcgccccg cgtgaaggac attctgttca ccgctcggct cgtcgaggcg 540
caggaggctc tggcgatcgg cctcgtcaac gaggtggtcg aggatgccgc ggccgtcgcg 600
gcccgagcgg atgcgctggc caccctgctc gcgagccacg cgcccctcac cctccaggcc 660
accaaggaag gcctgcgccg catcggcgag gagggcgcgg cggaggccgc cgagggcgag 720
cggcccggcg acgacctgat cgtgatgacc tatatgagcg cggatttccg ggagggcatg 780
gaagccttcc tgggcaagcg cccgccgaac ttcaaagggc gctga 825
<210> 118
<211> 1224
<212> DNA
<213> Clostridium sporogenes
<400> 118
atgagtgata gaaataagga agtaaaagaa aaaaaggcaa agcattatct tagagagatt 60
actgcaaagc attacaaaga agctctcgaa gcaaaagaaa ggggagaaaa ggttggttgg 120
tgtgcatcta acttcccaca agaaatagct acaacattgg gggtaaaagt tgtttatcca 180
gaaaatcatg cagcagctgt agcagctaga gggaatggac aaaatatgtg tgaacatgct 240
gaggctatgg gtttttctaa tgatgtatgt ggttatgcaa gagtaaattt agctgttatg 300
gacataggtc atagtgaaga tcaaccaata cctatgccag actttgtact ttgctgtaat 360
aacatttgta atcaaatgat taaatggtat gagcatatag caaaaacttt agatatacca 420
atgattctta tagatatacc atacaataca gaaaatactg tttcacaaga tagaattaaa 480
tatattagag cacaatttga tgatgcaata aaacaattgg aagaaataac aggcaaaaaa 540
tgggatgaaa ataaatttga agaagttatg aaaatatccc aagaaagtgc aaaacaatgg 600
ttaagagcag catcctatgc aaagtataaa ccttcaccat ttagcggatt tgatttattt 660
aatcatatgg ctgtagcagt ttgtgcaaga ggtacacaag aagctgcaga tgcatttaag 720
atgttagcag atgaatatga ggagaatgta aaaactggaa aatccactta taggggagaa 780
gaaaaacaac gtatattatt tgaagggatt gcctgttggc catatttgag acataaatta 840
actaagctta gtgaatatgg tatgaacgta actgcaactg tatacgcaga agcctttggt 900
gttatatatg agaatatgga tgaattaatg gctgcttata ataaagttcc taattcaatt 960
agttttgaaa acgcattaaa aatgagatta aatgctgtta caagcactaa tacagaaggt 1020
gctgttattc atataaatag aagctgtaaa ttatggagtg gatttttata tgagctagca 1080
agaagattag aaaaggaaac aggaattcct gtagtatcat ttgatgggga ccaggcagac 1140
ccaagaaatt tctcagaagc tcaatatgat actagaattc aaggacttaa tgaagtaatg 1200
gttgctaaaa aggaggctga ataa 1224
<210> 119
<211> 1125
<212> DNA
<213> Clostridium sporogenes
<400> 119
atgtcaaatt cagataaatt ttttaatgac tttaaggata ttgtagaaaa tcctaaaaaa 60
tatataatga agcatatgga acaaactgga caaaaggcta taggatgtat gccattatat 120
actcctgagg aacttgtatt agctgctgga atgtttccag taggggtatg gggaagcaat 180
acagaacttt caaaagctaa aacatatttc ccagcattta tttgttcaat attacaaaca 240
acattggaaa atgcattaaa tggagaatat gatatgttat ctggtatgat gattacaaat 300
tattgtgatt cattaaaatg catgggacaa aattttaaac taaccgttga aaatattgag 360
tttatcccag taacagttcc acaaaataga aaaatggaag ctggaaaaga gtttttaaaa 420
agtcaatata aaatgaatat tgagcaatta gaaaagattt ctggtaataa aataacagat 480
gaatctttag aaaaagctat agaaatatat gatgaacaca gaaaagtaat gaatgacttt 540
tcaatgttag catcaaaata tccaggtata ataacaccaa ctaaacgtaa ttatgttatg 600
aaatctgctt attatatgga taaaaaagaa catactgaaa aagttagaca attaatggat 660
gaaattaaag ctatagaacc aaaaccattt gaaggaaaga gagttataac tacaggtata 720
attgcagatt cagaagattt acttaaaata ttagaagaaa ataatatagc tatagttggt 780
gatgatatag cacatgaatc tagacaatat agaacattga ctccagaagc gaacacacca 840
atggataggt tagctgagca atttgctaat agagaatgta gtactttata tgatcctgaa 900
aagaaaaggg gtcaatatat agtagaaatg gctaaagaga gaaaagcaga tggaattata 960
tttttcatga caaaattctg tgacccagag gaatatgatt atccacaaat gaaaaaggat 1020
tttgaagaag caggcattcc acatgtacta atagaaactg atatgcaaat gaaaaattat 1080
gaacaagcta gaactgcaat tcaggctttt tcagaaacac tttaa 1125
<210> 120
<211> 795
<212> DNA
<213> Clostridium sporogenes
<400> 120
atggcagaca tttatactat gggtgtagac ataggttcaa ctgcatcaaa aacagtagta 60
ttaaaaaatg gtaaagaaat tgtaagtcaa gcagtaataa gtgtaggggc cggaacaagt 120
ggccccaaga gagctataga ttctgtatta aaagatgcta aattatccat tgaagattta 180
gactatattg tatccactgg atatggaaga aatagtttcg attttgctaa caaacaaatt 240
tctgaattaa gttgtcatgc aaaaggggtc tatttcgata acaataaagc tagaacagtt 300
attgatatag gcggacaaga tattaaagta ttaaaattag cggatagtgg aagactttta 360
aactttataa tgaatgataa atgtgctgca ggaacgggac gatttttaga tgtaatgtct 420
agagtaatag aagttccagt tgatgagtta ggaaaaaaag cattagaaag caaaaatcct 480
tgtactatta gttctacctg tacagtattt gcagagtcag aagtaatttc tcaacttgca 540
agaggagtta aaactgaaga tttgatagca ggaatttgta aatctgtagc atcaagagtg 600
gctagccttg caaagagaag tggtatagaa gaattagtag ttatgagtgg aggagtagct 660
aaaaatatag gtgtagtaaa ggcaatggaa gcagaattgg gaagagacat atatatatct 720
aaaaattctc aattaaatgg agcattggga gcaagtctat acgcttatga aagttttcaa 780
aaagaaagga gctaa 795
<210> 121
<211> 1239
<212> DNA
<213> Clostridium sporogenes
<400> 121
atggaaaaca atacaaatat gtttagtgga gtaaaggtta ttgaattagc aaattttata 60
gctgctccag cagcaggtag attttttgct gatggtggtg cagaggtaat aaaaattgaa 120
tcacctgctg gagatccttt aagatatact gctccttcag aaggaagacc attaagccaa 180
gaagaaaata ctacttatga tttggaaaat gcaaataaaa aagcaatagt attaaatctt 240
aaaagcgaaa aaggtaaaaa gatattacat gaaatgttag cagaagcaga tatattatta 300
actaattgga gaacaaaggc tttagttaaa caaggattag actatgaaac actaaaagaa 360
aaatatccta aattagtttt tgcacaaata actggttatg gtgaaaaagg accagataaa 420
gatcttccag gctttgatta tactgcattt ttcgctagag gcggtgtttc aggtactctt 480
tatgaaaaag gaactgtgcc tccaaatgtt gttccaggac ttggagacca tcaagctggg 540
atgtttttag cagcgggtat ggcaggagct ttatataaag caaaaacaac aggacaagga 600
gataaagtaa cagtaagttt aatgcatagt gctatgtatg gactaggtat tatgatacaa 660
gctgctcaat ataaagatca tggattagta tatccgataa atcgtaatga aactccaaat 720
ccttttatag tttcatataa atctaaggat gattactttg ttcaagtatg tatgccacca 780
tatgatgttt tctatgatag atttatgacc gctttaggaa gagaagattt agttggagac 840
gaaagataca ataaaataga aaatttaaaa gatggacgtg ctaaggaagt atacagtata 900
atcgaacaac aaatggttac aaagacaaag gatgaatggg ataacatatt tagagatgca 960
gacattccat ttgctatcgc acaaacttgg gaagatttat tagaagatga acaagcttgg 1020
gcaaatgatt atttgtataa gatgaaatat ccaacaggaa acgaaagagc attagtaaga 1080
cttccagtat tctttaaaga agcaggatta ccagaatata atcaatcacc acaaatagca 1140
gaaaatactg tagaagtttt aaaagaaatg ggatatacag aacaagagat tgaggaatta 1200
gaaaaagata aagatataat ggtaaggaag gaaaaataa 1239
<210> 122
<211> 1107
<212> DNA
<213> Lachnoanaerobaculum saburreum
<400> 122
atgtggcatt gtttagaaac tttaaaaaag attagtgcgt ctccaaagga acagcttaat 60
aaataccttg aagaaggaaa aaaagttatt gctgttgcac cggtttatac acctgaggag 120
attatccatg cttttggatt tgtacctatg ggggtatggg gcgcagatat tgaaattaat 180
gagtcaaaaa aatattatcc tgcatttatt tgctcaataa tgcagacagt attggagctg 240
ggaataaagg gaaattataa cggagttagt gctatagtgg ttccttcgct atgtgactca 300
ttaaaaactt tgggacaaaa ttggaaatat gcggtaaagg acattccttt tataccaatg 360
acctatccac aaaatagaaa atctgattat gctgttgatt tcacattgga gatgtataag 420
agagtgatca gtgatttgga aaatattacc ggagaaaagt ttgatgaagg taaactcaaa 480
aacacttatg aaatttataa tgagcataat agggttatga gagaatttac aaaagtttcg 540
gaagagtatg aagtttcggc aacagataga agtgcagtat ttaaaagtgc ttggtttatg 600
cttaaggagg aacatacaga acttgttagg gaattgatcg aacttataaa aaaagagggt 660
aaaatatcta agaagctaag aatttataca acaggaatat tggcggatgc accggattta 720
ctcaatattt ttgacagcaa taatatgcaa atcgtaggtg atgatattgc ttatgaatcc 780
agacagtata gaacagatat acccgatgga aatggtttat atgctcttgc aaagaagttt 840
tcaaatatgg acaactgtac tcttttatat gataaggata agagaagggt tgactttatt 900
attgaagaag caaagaaaaa aagagctgac ggaatagtag ttcttatgac caagttttgc 960
gatcctgaag aatttgacta tgtgcctata aagagggcgg caaatgaagc aggtattcca 1020
catatcaata tagaagtgga tagacaaatg aaaaattatc aacaggcaaa tactatgtta 1080
caaacatttg cagacatgtt ggtttag 1107
<210> 123
<211> 1230
<212> DNA
<213> Lachnoanaerobaculum saburreum
<400> 123
gtggaagaag ctaaaaaaca aaagcctaca gttgatccaa acagcgcaaa ggctagattg 60
ggcaggatag cagcaaaagc atatagtgac tgtgttgagg ctaaaaagcg aggagaattg 120
gtaggatggt gtgcaagtaa ttttccggtg gagatacctg agaccttggg attgtacgta 180
tgttaccctg agaatcaggc ggcaggtatt gctgccagag gcggtggaga acgaatgtgc 240
agtgagagtg aaggtgacgg atactctaat gatatatgcg catatgcaag aatttcgctt 300
gcatatatga agctgaagga agctcctgaa caggatatgc cacagcctga ctttgttcta 360
tgttgtaata atatatgcaa ctgcatgatt aagtggtatg aaaatatagc aaaagaactt 420
aatattccta tgattatgat tgatatacct tttaatcctg attatgaagt ttcagatgct 480
atgacagcat atatcagaaa tcagttttgg gatgcaatac atcaattgga ggaaattaca 540
ggcaaaaaat ggagtaatga aagatatgaa gaggtaagga aaatatcagg aagaagctcc 600
agagcatggc ttgaggctac agcgactgcc aaatattcac catctccgtt taacggattt 660
gatttattaa atcatatggc ggttatggtt actgccagag gaaaacttga agctgcagaa 720
gcaatggaaa cacttttgca ggagtacaag gataatcatg agaagggaga gtctacgttc 780
aagggagaag aaaaatatag aataatgttt gagggtatag catgctggcc atggcttcgt 840
gctactgcta caggacttaa gagtcgtgga atcaatatgg ttacaactat atatgcggat 900
gctttcggat ttatctatga tgactttgac ggaatgtgca gagcatatgc caatgttcct 960
aattgtatga atatagagca tgcaagagat aagagaataa aactttgtaa ggacaatagt 1020
gttgaagggc ttctcgttca cacaaacagg tcttgtaaac tttggtcagg atttatgtct 1080
gaaatgagca ggcaaatagg tgaagaatgt ggtattccgg ttgtaagctt tgatggagac 1140
caagcagatc caagaaattt ctcagaggct caatatgata cgagagttca gggattgaca 1200
gagataatgg aagcaaataa ggaaatttaa 1230
<210> 124
<211> 771
<212> DNA
<213> Lachnoanaerobaculum saburreum
<400> 124
atgtacacat tgggtgttga tataggctca actacatcca aagcggtaat attggaggat 60
ggagaaaata tagttgcatc ttcaattgtt atagcaactg taggaacggc aggagtagaa 120
gaggctgtaa aaaatgtact aaacttttca aaactcgaac taaatgacat taaagcagtg 180
gttgctacag gatatggaag aatgaattat gatgtagcag attacaaggt tagtgaattg 240
acatgtcatg cattaggtgt acataaggag ttcccgaatg tcagaactgt aattgatatc 300
ggaggtcagg atgccaaggt aatatctctt gcggcaaacg gtaagatgac aaattttgtt 360
atgaatgata aatgtgcggc agggacaggt agatttcttg atgtaatggc taatatatta 420
aatcttgata tacaggattt ggaggtggaa gccttaaaat cagataatcc ggcaaatata 480
tcaagtactt gtacagtttt tgcggaatcg gaagtcatat cacagcttgc tacaggaaga 540
aatattcctg atttggttgc agggatatgc aaatctgttg cagtaagggt tgccgccctg 600
gctaaacgag taggtatagt tgaagaagtg tgtatgagcg gcggagtggc aaaaaactcg 660
ggtgtgagga atgctatgag taaagagctt ggtgtagata tagtgtttag taaggatgct 720
caacttatgg gagcacttgg agccgcaata tacggtttta aaaagttata a 771
<210> 125
<211> 795
<212> DNA
<213> Peptostreptococcus stomatis
<400> 125
atgagcagtg tatacacaat gggtattgac attggatcaa catcatcaaa gtgtgtgata 60
atgaaggatg gtaaggaaat tgtaagtgaa ggtgtagtta gcttgggtgc tggaactaag 120
ggttctgacc tagttattga ggaagtgctt ggtaaggcag gaatgacttt cgatgaaata 180
gacctaatcg tatcgactgg atatggtaga aatagctatg aaagagctgc caagactgtt 240
agtgagctta gttgtcatgc caagggtggt ggatatatct ttggtggtgc cggaactatt 300
atagatatcg gtggtcagga tataaaggta ttgaagctaa atgacaaggg tggtcttgtt 360
aacttcctga tgaatgataa gtgtgctgcc ggtacaggta ggttcttgga agttatgtct 420
ggcgtattgg atgtaaagct agatgaacta ggggaactag atgccaaggc tacagaagtt 480
acaccaatca gttctacatg tacagttttt gctgagtcag aagttatatc atgtatggct 540
aagaagattc ctctagaaaa tatcataaga ggtatacacg catctgttgc aacaagggtt 600
gctagtttgg caagaagagg tggtttgaag actcctgtag ccatgacagg tggagttagt 660
aagaacaagg gtatagtaag ggctcttaaa gaagaactag aatgtgatat cttgatatct 720
cctgattctc agatggctgg tgctataggt gcagccctat atgcatatga cgaataccag 780
aagcaaaacg cttaa 795
<210> 126
<211> 1119
<212> DNA
<213> Peptostreptococcus stomatis
<400> 126
atgagtaata tagatgtatt gttaggtaaa cttgatgtaa gtcttttggg acaggtagac 60
aagtatgttt cagaaggtaa gaaggtaata ggttgcgcgc cagtttatac accagaagaa 120
ttagtatatg ctgcaggcat ggtaccaatt ggtgtatggg gtgcagaagg tgaagtaggt 180
ctatcaaagg aatacttccc agcattttat gcagctataa tccttagatt aatggacctt 240
ggtttagaag gtaagcttga caagatgtca ggtatgatta taccgggact aagtgacggt 300
ctaaagggac ttagccagaa ctggaagagg gctataaagc aggttccggc cctatacata 360
ggctatggtc agaacagaaa aattgaagct ggtattactt acaatgaaaa gcagtacatc 420
aagctaagag gacagttaga agaaatagct ggttgcaaga tagaagatgc taaggttgaa 480
gaggctatag ttctttacaa caagcacaga aaggcaatgc aggaattcag ttctctagca 540
gctagtcact taaatactat tacacctatt ctaagagcta gagtaatgac aagtgccttc 600
ttgttcgaca aggcagaaca tttagctata ttggaagaat tgaataaaga attaaaggcg 660
ttacctgaag aaaaatttgc tggcaagaag gtagttacta ctggtattct tgcaaatagc 720
ccaggtatgc tagaaatact agatgagtac aaacttggta tagttgatga caatatcaac 780
catgaatcag gccagtttga ctacctagtt gatgaaggta ctggtaatcc agttagagcc 840
ttatctaagt ggatttcaga tatagaagga agtactttgt tgtatgatcc agaaaaactt 900
aggggacaga taataattga caaggttaag aagcatcagg cagatggtgt tatataccta 960
atgactaagt ttagtgattc tgatgaattc gactatccaa tcatcagaaa agaattagaa 1020
aatgcaggta tcttgcatat actagttgag gttgatcagc aaatgactaa ctttgaacag 1080
gcgaaaacag cattacagac tttcgctgat atgatttaa 1119
<210> 127
<211> 1236
<212> DNA
<213> Peptostreptococcus stomatis
<400> 127
atgagtaata caggaatggt agaagaaaag ccggcaaaag tattgttagg agaaattgtt 60
gcaaagcact ataaggaagc ttgggaggca aagaataatg gtgaactagt tggatggtgt 120
gcatctaact tcccacagga aatattcgaa actatggata taaaggttgt ttatccagaa 180
aaccaggctg ctgctatatc tgctaagggt ggcggacaga gaatgtgcga aatagctgaa 240
aatgaaggat attcaaatga tatctgtgct tacgctagaa tatctttggc atacatggac 300
gttaaggatg ctccagaatt aaatatgcca cagccagact tcgttgcttg ctgtaacaat 360
atttgtaact gtatgatcaa gtggtatgaa aatatagcta aggaattgaa tataccttta 420
attttaatag acgttcctta caacaatgac tacgaggctg aagacgatag agttgaatat 480
ctaagaggtc agtttgatta tgctatcaag cagttagaag aactaactgg caagaagtgg 540
gatgaaaaga agtttgaaga agtaatggaa gtttctcaga gaacaggtag ggcttggtta 600
aaggctactg gatatgctaa gtatactcca tcaccattct caggctttga cgtattcaac 660
cacatggctg ttgcagtttg tgcaagaggt aagatagaat cagctatagc attcgaaaag 720
ctagctgaag aatttgacga aaacgtaaga actggtaagt caacatttaa gggcgaagaa 780
aagttcaggg tgttatttga aggtatagca tgttggccac acctaagaca tacattcaag 840
cagcttaagg atgctggtgt taatgtctgt ggtacagtat atgcggatgc tttcggatat 900
atctatgaca atacatatca gttaatgcag gcttactgcg gaactccaaa tgctatttca 960
tacgaaaggg caactgatat gagactaaag gttattgaag aaaacaatat agatggtatg 1020
ttaatccaca tcaacagaag ttgtaagcag tggtcaggta tcatgtacga gatggaaaga 1080
gatattagag aaaagactgg tataccaaca gctacattcg atggtgacca ggccgatcca 1140
agaaacttct ctgaagctca gtatgatact agagtacagg gtcttataga actaatggaa 1200
gctaataaag ctgcaaagat gaaggaggcg cactaa 1236
<210> 128
<211> 1227
<212> DNA
<213> Clostridium difficile
<400> 128
atgtctgaaa aaaaagaagc tagagtagta attaatgatt tattagctga acaatatgca 60
aatgcattta aagctaaaga agaaggaaga cctgtaggtt ggtcaacatc agtatttcct 120
caagagttag cagaagtatt tgacttaaac gtattatatc cagaaaacca agcagctgga 180
gtagcagcta aaaaaggttc tttagaatta tgtgaaatag ctgaatctaa aggatattct 240
attgacctat gtgcatatgc aagaacaaat tttggtcttt tagaaaatgg tggatgtgaa 300
gctttggata tgccagctcc agatttccta ctttgctgta acaatatatg taaccaagtt 360
ataaaatggt atgaaaatat ttcaagagaa ttagatatac ctttaataat gattgataca 420
actttcaata atgaagacga agttactcaa tcaagaatag attatattaa agctcaattt 480
gaagaagcta taaaacaact agaaattata tcaggaaaga aatttgaccc taagaagttt 540
gaagaagtaa tgaaaatatc agctgaaaac ggaagactat ggaagtattc tatgagttta 600
ccagcagatt cttctccttc tccaatgaat ggatttgact tatttactta catggctgta 660
atagtttgtg ctagaggtaa aaaagaaact acagaagcat ttaagttact tatagaagaa 720
ttagaggaca acatgaaaac tggtaaatct tctttcagag gggaagaaaa atacagaata 780
atgatggaag gtataccttg ttggccatat ataggataca agatgaaaac attagctaaa 840
tttggagtta acatgacagg tagtgtttac ccacatgctt gggcattaca atatgaagtt 900
aatgatttag atggaatggc agtagcatat agtactatgt ttaacaatgt aaacctagac 960
cgtatgacaa aatatagagt tgattcttta gtagagggta aatgtgatgg agcattctat 1020
catatgaaca gaagctgtaa acttatgagt ttaatacaat atgaaatgca aagaagagca 1080
gctgaagaaa ctggattacc atatgctgga tttgatggtg accaagcaga ccctagagct 1140
ttcactaatg ctcaatttga aacaagaatt caaggtttag ttgaagtaat ggaagaaaga 1200
aaaaaactta atagaggtga gatataa 1227
<210> 129
<211> 1125
<212> DNA
<213> Clostridium difficile
<400> 129
atggaagcta ttttatctaa aatgaaagaa gtagttgaaa atccaaatgc ggctgtaaaa 60
aaatataaaa gtgaaactgg taaaaaagct ataggttgtt tcccagttta ttgcccagaa 120
gaaattatac atgcagctgg aatgcttcca gttggtatat ggggaggaca aacagaatta 180
gatttagcta aacaatattt ccctgcattt gcatgttcaa taatgcaatc atgtttagaa 240
tatggattaa aaggtgctta tgatgaatta tctggagtta ttataccagg tatgtgtgat 300
acactaattt gtttaggaca aaactggaaa tcagcagtac ctcatataaa atatatatca 360
ttagtacacc cacaaaatag aaaacttgaa gctggtgtaa aatacttaat cagtgagtac 420
aaaggcgtaa aaagagaact tgaagaaatt tgtggatatg aaatagaaga agcaaaaatt 480
catgaaagta tagaagttta caatgaacat agaaaaacta tgagagactt tgttgaagta 540
gcttataaac attctaatac tataaaacca tcaataagaa gcttagtaat taagagtggg 600
ttctttatga gaaaagaaga acatactgag ctagtgaaag atttaatagc aaaattaaat 660
gctatgccag aagaagtctg ttctggaaag aaagttttat taacaggtat attagctgat 720
tctaaagata tattagacat tttagaagac aacaatatat cagttgtagc tgacgactta 780
gcacaagaaa caagacaatt cagaacagat gtaccagcag gtgatgatgc gttagagaga 840
ttagcaagac aatggtcaaa catagaagga tgttcattag cttatgaccc taagaaaaaa 900
cgtgggtcac ttatagtaga tgaagttaaa aagaaagata tagatggtgt tatcttctgt 960
atgatgaaat tctgtgaccc agaagaatac gattatcctt tagttagaaa agatatagaa 1020
gatagtggaa tacctacttt atatgttgaa atcgaccaac aaactcagaa taatgaacaa 1080
gccagaactc gtattcaaac ttttgctgag atgatgagtt tagcg 1125
<210> 130
<211> 798
<212> DNA
<213> Clostridium difficile
<400> 130
atgtacacaa tgggattaga tataggttca actgcatcaa agggagtaat cttaaagaat 60
ggggaagata ttgtagcttc tgaaacaata tcctctggta ctgggactac tggaccatca 120
agagttttag aaaaattata tggcaagaca ggtcttgcaa gagaagatat taaaaaagtt 180
gtagttacag gatatggaag aatgaactat tcagatgctg ataagcaaat aagtgaatta 240
agctgtcatg ctagaggggt aaatttcata attccagaga caagaaccat tattgacata 300
ggtggtcaag atgcaaaggt attaaaatta gataataatg gaagactatt aaactttctt 360
atgaatgaca aatgtgctgc aggtacagga agatttttag atgtaatggc aaaaataata 420
gaggttgatg tatctgaact cggaagtata tctatgaatt ctcaaaatga agtatcaata 480
agcagtacat gtacagtatt tgcagagtct gaggttatat cacatttatc tgaaaatgca 540
aaaattgaag atatagtggc aggtattcat acttcagtag caaagagagt ttctagccta 600
gtaaaaagaa taggagtaca aagaaatgta gttatggttg gtggggttgc tagaaatagt 660
ggtattgtaa gagctatggc aagagaaatc aacacagaaa ttattgtacc tgatatacct 720
caattaactg gtgctttagg agcagcgtta tatgcttttg atgaagcaaa agaatcacaa 780
aaagaagtga aaaatata 798
<210> 131
<211> 1194
<212> DNA
<213> Clostridium difficile
<400> 131
cttttagaag gagttaaagt agtagaactt tcaagtttca tcgcagcacc atgttgtgca 60
aaaatgttag gtgactgggg tgcagaggtt attaagattg aacctataga aggtgatgga 120
ataagagtta tgggtggaac atttaaatct ccagcatcag atgatgaaaa ccctatgttt 180
gaattagaaa atggaaataa aaagggtgta agtattaatg taaaatcaaa agaaggagta 240
gaaatattac ataaattatt atcagaagca gacatatttg taactaatgt tagagttcaa 300
gcattagaaa aaatgggtat agcttatgac caaataaaag ataagtatcc aggattaata 360
ttctctcaaa tattaggata tggtgaaaaa ggacctttaa aagataaacc aggatttgac 420
tatactgcat acttcgcaag aggaggagtt agccaatctg ttatggaaaa aggaacatct 480
ccagcaaata cagcagcagg atttggtgac cactatgcag gtctagcact agcagcagga 540
agtttagcag cattacataa aaaagctcaa actggtaaag gtgagagagt aacagtaagt 600
cttttccata cagctatata tggaatggga acaatgataa caacagcaca atacggaaat 660
gaaatgcctt tatcaagaga aaatccaaac agcccattaa tgactacata taaatgtaaa 720
gatggaagat ggattcaatt agctttaata caatacaaca agtggttagg caaattctgt 780
aaggttataa atagagaata tatattagaa gacgatagat ataataacat agattcaatg 840
gttaatcatg ttgaagattt agttaagata gttggagaag ctatgttaga aaaaacatta 900
gacgagtggt cagctttatt agaagaagca gacttaccat ttgaaaaaat tcaaagctgt 960
gaagatttat tagatgacga acaagcttgg gcaaatgact tcttatttaa gaaaacatac 1020
gatagcggaa atacaggtgt cttagttaat actccagtta tgtttagaaa tgaaggaatt 1080
aaagaatata caccagcacc aaaagtaggt caacatactg tagaagtatt aaaatcttta 1140
ggctacgatg aagagaaaat aaataacttt aaagatagta aagttgtaag atat 1194
<210> 132
<211> 768
<212> DNA
<213> Escherichia coli (strain K12)
<400> 132
atgagcgaac tgatcgtcag ccgtcagcaa caagtattgt tgctgaccct taaccgtccc 60
gccgcacgta atgcgctaaa taatgccctt ctgacgcaac tggtaaatga actggaagct 120
gcggctaccg atagcagcat ttcggtctgt gtgattaccg gtaatgcacg cttttttgcc 180
gctggggccg atctcaacga aatggcagaa aaagatctcg cggccacctt aaacgataca 240
cgcccgcagc tatgggcgcg attgcaggcc ttcaacaaac ctctcatcgc agccgtcaac 300
ggttacgcgc ttggtgcggg ttgcgaactg gcattgttgt gcgatgtggt ggttgccgga 360
gagaacgcgc gttttggttt gccggaaatc actctcggca tcatgccagg cgcaggagga 420
acgcaacgtt taatccgtag tgtcggtaaa tcgttagcca gcaaaatggt gctgagcgga 480
gaaagtatca ccgctcagca agcacagcag gccgggctgg ttagcgacgt cttccccagc 540
gatttaaccc tcgaatacgc cttacagctg gcatcgaaaa tggcacgtca ctcgccgctg 600
gccttacaag cggcaaagca agcgctgcgc cagtcgcagg aagtggcttt gcaagccgga 660
cttgcccagg agcgacagtt attcaccttg ctggcggcaa cagaagatcg tcatgaaggc 720
atctccgctt tcttacaaaa acgcacgccc gactttaaag gacgctaa 768
<210> 133
<211> 774
<212> DNA
<213> Rhodobacter capsulatus
<400> 133
atgagctatc acacgatccg ctacgagatc tccgaagggc tggcggtgat cacgctcgat 60
cgccccgagg tgatgaatgc gctgaacgcg gcgatgcggc acgaattgac cgcggcgctg 120
caccgcgcgc ggggcgaggc gcgggcgatc gtgctgaccg gatcggggcg ggccttttgc 180
tctgggcagg atctgggcga tggcgcggcc gaggggctga acctggaaac cgtgctgcgc 240
gaggaatacg agccgctttt gcaggcgatt tacagctgtc cgctgccggt tctggcggcg 300
gtgaacggcg cggcggcggg ggcgggggcc aatctggctc tggcggccga tgtggtgatc 360
gcggcgcaat ctgcggcctt catgcaggct ttcacccgga tcgggctgat gccggatgcg 420
ggcgggacct ggtggctgcc gcggcaggtc ggcatggccc gcgccatggg gatggccctg 480
ttcgccgaga agatcggcgc cgaagaggcc gcgcgcatgg ggctgatctg ggaagccgtg 540
cccgatgtcg atttcgagca tcactggcgg gcccgggcgg cgcatctggc gcggggccct 600
tcggcggcct ttgcggcggt gaagaaggcc tttcatgccg gtctgagcaa tcccctgccc 660
gcgcagctgg cgctggaagc ccggttgcag ggcgaactgg gccagagcgc ggatttccgc 720
gagggcgtgc aggcctttct ggaaaagcgc ccgccgcatt tcaccgggcg ctag 774
<210> 134
<211> 2106
<212> DNA
<213> Pseudomonas stutzeri
<400> 134
atgacggatg tcattcggct cgaacgccgg ggcgatatcg ctctgatcct ggtcaacaac 60
ccgccggtca acgcccttgg ccatgccgta cgaaaaggcc tgttggatgc ctttcaagag 120
gctgacgagg cgcccgaggt gacggccgtg gtgctggtct gcgaaggccc gaccttcatg 180
gccggcgccg atatcaagga gttcggcaaa ccgccgcagg caccgagcct gccggaggtg 240
atcgaggtga tcgagggctg ccgcaagccg agcgtcgcgg tgatccacgg caccgccctg 300
ggtggtgggc tggaggtcgc gctgggctgc cattaccgta tcgcccggtc ggacgccaag 360
gtcggcctgc cggaggtgaa gctgggcctg ctgcccggcg ccggcggtac ccagcgcttg 420
ccgcggctgg ccggtgtcga gaaggcgctg gagatgatcg tcagcggcca gcccatcggt 480
gcggcggagg cgctggagca ctatatcgtc gacgagctgt tcgaaggcga tctgatcgag 540
gccggtctga cctatgcgcg tcgccttgtc gaggagggcc gcggtccgcg ccgcagtggc 600
gagcagaccc gcggtctgga aggcgtcgac aacgaggcgc tgattcgcgc caagcacgcc 660
gaggtggcca agcgcatgcc ggggctgttc tcgccgctgc gctgcattgc cgcggtggaa 720
gccgccacca ggctgccgct ggccgaaggc ctcaagcgcg agcgcgagtt gttcaccgag 780
tgcctgaatt caccgcagcg cggcgcgctg atccattcgt tcttcgccga gcgtcaggcc 840
ggcaagatcg acgacctacc atccgacgtc accccccgcc cgatcaggac cgccgcggtg 900
atcggcggcg gcaccatggg cgtcggcatc gccttgagct tcgccaacgc cggggtgccg 960
gtgaagctgc tggaaatcaa tgacgaggcg ttgcaacgcg gcctgcagcg tgcccgcgaa 1020
acctacgcgg cgagcgtcaa gcgcggcagc ctgaccgagg atgcgatgga gcagcgcctc 1080
gcgctgatcg ctggcgtcac cgactacggc gccctggctg atgccgacgt ggtggtcgag 1140
gccgtgttcg aagagatggg cgtcaagcag caggtcttcg agcaactgga tgcggtgtgc 1200
aagccgggtg cgatcctcgc ctccaacacc tcgtcgctgg acctgaacgc catcgccggc 1260
ttcaccaggc gccccgagga tgtggtcggc atgcacttct tcagcccggc caatgtcatg 1320
cgcctgctgg aagtggtgcg cggtgagcgg accagcgatg aagtgctcgc cgccgccatg 1380
gcgatcggca agcagctgaa gaaggtctcg gtggtggtcg gcgtctgcga cggcttcgtc 1440
ggcaaccgca tggtcttcca gtacggccgc gaggcggagt tcctgctgga ggaaggcgcc 1500
acgccacaac aggtcgacgc tgccctgcgc aatttcggca tggccatggg accgttcgcc 1560
atgcgcgatc tgtccggtct cgacatcggc caggcgatcc gcaagcgcca gcgcgcgacg 1620
ctgccggcgc acctggattt tcccaccgtc tcggacaagc tctgcgccgc cggcatgctg 1680
gggcagaaga ccggtgccgg ctactaccgc tacgaacccg gcaaccgcac cccgcaggag 1740
aatcccgacc tcgcgcccat gctggaagcc gcgtcgcggg aaaagggcat cgagcggcag 1800
gcgctggacg agcagtacat cgtcgagcgc tgcatcttcg cgctggtcaa cgagggcgcg 1860
aagattctcg aggaaggcat tgcccagcgc tccagcgaca tcgacgtcat ctacctcaac 1920
ggctacggct tcccggcctt ccgcggcggg ccgatgtact acgccgacag cgtcggcctg 1980
gacaaggtgc tggcgcgagt aaaagaactg cacgcgcgtt gcggcgactg gtggaagccg 2040
gcgccactgc tggaaaaact ggccgccgaa ggccgcacct tcaccgaatg gcaggccggg 2100
caatga 2106
<210> 135
<211> 1968
<212> DNA
<213> Haliangium ochraceum
<400> 135
atgatcgtcg gagtcatcgg gtcgggcgcc atcggcccag acctcgccta cggattcgcc 60
tcggccctgg ccagcgttcc cggcgccagg gtctatctac acgatatcaa gcaggaggcc 120
ctcgacgccg gtatgcagcg catccgcggc tacatcgcca agggcctggc ccgcggcaag 180
atcagcgaac gcgtcgccgg cgccctggag acggtgctcg tgcccacgct ctcgctcgcc 240
gatctcgcgc cgtgcagcta cgtgctcgag gccgccaccg aggagctcgg ggtcaagcgc 300
gccatcttgc gcagcctcga ggatacagtc gatagcgagt gcctcatcgg cttcgccacc 360
tcgggcctgc cgcgcgcgat catcgccgcc gaggtcaaac atcccgagcg ctgcttcgtc 420
aatcacccct tctaccccgc ctggcgttcg ctgcccgtcg aggtcgtgct ctcgggtagc 480
ccggcgcacg gccagcgcat gctggccacc ctcgaggccc tgggcaaagt ccccgtcatc 540
accgcggacg cgccctgctt cgcggccgac gacatctttt gcaactactg ctcggaggcc 600
gcgcgcatcg tcgaggaagg catcgccaat cccgcccagg tcgacgccat cgtccacggc 660
gccatcggcg gcggcggccc gctcaacgtc ctcgacgcca cccgcggcaa cctgctcacc 720
gtgcactgcc aggagctgat gcgcgacgcc gacaccggca cgccgtggtt cgagccgccc 780
gccatcctgc gcgagcgcgg cgacgccctg tggcacgatc ccaaggcccc gcacgacccc 840
gccttcgacg aggccctgcg cgagcgcgtg ctcgaccgca tcctggccgt gctgctcgcg 900
cgcacagtgt tcgtgctcga tcacggcatc tgcgccgcca ccgagctcga ctggatgacg 960
cgcaccgcgc tcggcttccg caccggcttg gtcgacctgg tggacgaact cggccccgag 1020
cgcgtggccg agctgtgcca gcgctacgcc gccgagcacc ccggcttcgt catcccggac 1080
agcatccgcg agcagcacaa gccgcgcttc tacggcaacc tgcgcgtcac ccgccaggac 1140
gagctggcca tcgtgcgcat cttccgcccc gaggtgaaga acgcgctcga ccgccgcacc 1200
ctgagcgagc tcgaccacct catggccgcg ctgtcggccg acgacagcgt cgagggcgtg 1260
gtcctgagca gcgccggcgg cgcgctggcc ggcgccgaca tcaccgagct agcgcgcgtg 1320
cgcaccaccg aggaggcggt gtccacctgc gctttcggac aagcggtctt gaaccgcatc 1380
gcggccatgg acaagcccgt ggtcgccgcc gtcgacggcc cggtgctggg cggcggcgcc 1440
gagctgtcga tggcgtgcca tgcgcgcgtc gtcggcccgc gcctgagcat gggccaaccc 1500
gaggtcaacc tcggcatcat ccccggctac ggcggcaccc agcggctgcc gcggctcatc 1560
ggcgtggagc gcgcgctggc catgatgcgc acggcgcaga gcatcgacgc gcagaccgcg 1620
tgcgagtggg gctgggccag cggcacgccg atggtcgact tcgtcggcgc ggccgcgacc 1680
ctcatccgca gccacctcgc cggcgaggcc gagctcgcgc cgctcgaccc cgcgcccatg 1740
agcgtacccg ccgcggccgc ccccgtggac atcggccacc gctcgcgcgt catcgacgag 1800
atcctcgtgg atgtggtcca gtccggcttg cgcgcgccgc tgagcgaggg cctggccacc 1860
gaggccgccg gcttcggccg ctgcgtgctc accgtggacc tcgacatcgg actcaagaac 1920
ttcatgcaga acggcccccg ggttccggcg ctgttcctcc acgagtag 1968
<210> 136
<211> 768
<212> DNA
<213> Anoxybacillus flavithermus
<400> 136
atgttttcta ttcaacaaga ggggtatgtg gcgattttag cacttcatcg tccaccagca 60
aacgctttag catcttctgt tttgaaagag ctttcagaac ggcttgatgc attaaaagaa 120
gacgaacaag tacgtgtcat cgttcttcac ggagaaggaa gatttttctc agctggtgcc 180
gatattaaag agtttacagc gatcgaggcg agcgaacaag cggctgaact tgctcgagct 240
ggacaacaag tgatggagaa aattgaacag tttccgaaac cgattattgc cgcgattcac 300
ggtgctgcac ttggcggagg gctcgagtta gctatgagtt gccatctgcg catcgtagcg 360
gaaaacgcca aacttggctt accagaattg cagctcggca tcattccggg atttgcagga 420
acacaacgct tattgcgtca tgtcggtatg gcaaaagcgc tagaaatgat gtggacaagc 480
gaaccgatca caggtgcaga agctgtgcag tggggactag caaacaaagc cgtcccagaa 540
gaacaattgc ttgatacagc gaagcaactt gcacaaaaaa ttgctcaaaa gagcccgatt 600
tctgttcaag cggtattgaa actagttaat gaagctcgca caaaaacgtt ccatgaatgc 660
gttgaaaaag aggctcaact gtttggacaa gtctttgtaa cagaagatgc gaaagagggc 720
atttcggcat ttatcgaaaa acggacacca cagtttcaag gaaaataa 768
<210> 137
<211> 783
<212> DNA
<213> Streptomyces avermitilis
<400> 137
atgagcacgg cgcccgaagc tgccgacttg gtgctccacg agcgtcacgg cggcgtactg 60
accatcacca tcaaccgccc cgcgcagaag aacgccgtcg accacgaggc cgcggtacag 120
ctcgcggcgg ccgtggatct gctcgacgcg gacccggagc tgtcggtcgg cgtcctcacg 180
ggcgcgggcg gggtgttcag cgcgggcatg gacctgaagg cgttcgccaa gggcgagctg 240
cccttgctgc ccagccgggg cctgggcggg ctcacccgcg cgtcggtgcg aaagccgctg 300
gtcgccgcgg tcgagggctg ggcgctcggc ggtggcttcg agctggtcct cgcctgcgac 360
ctgatcgtcg ccgcggagga cgcccgcttc gggtttcccg aggtcatgcg tggtctcgtg 420
gcggcggagg gcggactggt caggctgccg cgccgacttc cgtaccacgt cgccgcgcgc 480
gtactgctga cgggcgagcc gctgaccgcc gtcgaagcca aggagtacgg gctcgtcaat 540
gagctgaccc cgcccggcgc cgcgctggac gcggcccggg agctcgcggg ccgcgtcgcg 600
cggaacgcac cgcttgcact ggcggccgtc aaggaggtcc tgcgcgagac acagggcctg 660
aaggagagcg acgcgttcag acgccaggac gagctcacga gcggactggc cgccagcgag 720
gacgcgcggg aaggcgcaca ggcgttcgcc gagaaacgcg ccccggtctg gcacggccgc 780
tga 783
<210> 138
<211> 1683
<212> DNA
<213> Advenella kashmirensis
<400> 138
gtggacaatg gccgtaagct gattgaacgt ggctggcatt tattcaaccg tatcgaaaag 60
ctagcctttc ctacactggc actcatgcac ggcccctgcc tgggtggcgg gctggaactg 120
gcactggcgt gccgttatcg aatcgcgatc gattctccca agccggtgat cggcctgcct 180
gaagtcaaat tgggcatctt ccccgcctgg ggcggcctga tgcgactacc ccgcctgatt 240
ggtccgcaaa ccgccctgaa catgatgctg accggtcgca cactggatgg ccgcaaggcc 300
aggtctgccg gtctggtaga tttgctggtc gcaccccgag ttgcagagaa atcggcgatc 360
gatctggtca cgtcgggcaa accggcgcgt caggctcgcg gcctggccgg cttgctcaat 420
cgtgcaccgt tcaagtcgct ggtggctgcc caggcacgca aaagcgtcaa gcaaaaagac 480
ccttatggcc actaccccgc caccctgacc atgctggatc tgtgggaaaa acatgatggc 540
gacccgttgg ccgatcccca ggcgctgacc cggctgctgc aatcggatgt cacccgcaat 600
ctgatccgtg tatttcacct gcaggagcgg ctcaaggcgt ttggcaagaa ggataatgcc 660
actcccgtca accatgttca tgtgatcggg gccggcgtga tgggcggtgg catcgctgcc 720
tggtgcgcgc tgcagggcat caaaaccacc ttgcaggata ccgacgccca gcgcatcgcc 780
ggggcgttca aaaacgccgt ctccatttat gcccgcaagg atcggtatac cgcgcaggca 840
gcccgcgatc gcctgattcc ggacctggcg ggccacggta tcgcgacggc tgatctggtg 900
attgaagcga tcagcgaaaa tccgcaagcc aagcaatcgc tctaccagca gatcgaacca 960
aaaatgaaag aaggcgccat tttagccacc aatacatcca gtctgtccat tgcgcagtta 1020
cgcagcgtgc tggtgcaccc cgaacgtttt gtcggtattc attttttcaa tccagtctca 1080
cgcatgccgc tggtagaagt ggtacatgcc gatggcatcg cccaggaaac tctggacacc 1140
gctgccgcct ttgtcggcaa aatcggcaaa ctgccgctgc cggttcagga cacgccgggc 1200
tttctggtca acgccgtgct tgctccctat atgctgcaag ccatgcggtg cattgacgaa 1260
ggcatggatc ccgaagtcat cgataccgca atgctggagt tcggcatgcc catggggccg 1320
atcacgctgg ccgatacggt tggtctggat attgccatgg cagccggcaa acagctgtcc 1380
gaaggccagg agccgccacg ctgcctgcaa gagaagattg cccaaggcaa gctgggtgtc 1440
aaaagcggcg aaggctttta cgtgtggaaa gaccgcaagc atgaccagcg cagtagcaaa 1500
gccatcccgc aaggcctggc acagcgcctg atcaagccgc tgatagagca gaccgaaaaa 1560
caacttgcga acaacatcgt gcaagatgca gatcttgccg atgcaggcgt gatattcgga 1620
accgggtttg cgccttttac cggaggaccc attcattaca aacaaagtaa aggaggacta 1680
tga 1683
<210> 139
<211> 714
<212> DNA
<213> Oligotropha carboxidovorans
<400> 139
gtgagccttt cgccgcttgc caacggcgta cgcgttctca cactggatcg tccgtccaag 60
gccaacgcgt tgaatgcgga ggtcgtggac cagttgcttg cgtgtgtcgc ccaggccgag 120
gcggaggatt gccgcgtgct gatcctcgcc gccaacggca aggcgttttg cggcgggttt 180
gatttcggtg gttatgaatc gatgtcggcg ggcgacctgc tgctgcgctt tgtccggatc 240
gaggagttgc tgcagcggat gcgccagtcg tcgtttgtca gcattgctct ggtgcatggt 300
gcggcgatgg gggcgggggc ggacatcgtc gcgtcttgca cctatcgcat cggcaccgac 360
gcaagccggt ttcgctttcc gggattccgt ttcggcgtgg cgcttggcac gcggcatctg 420
gcgcagcttg tcggcccgca acgggcgcgc gatatcctgc tgaccaatgc aacgatcgat 480
gcattgaccg ctgtcgatat cggattgctg acgcacctcg tcgatgccgg gagcatgcgg 540
cagaaagcgg acgagattat tgcgcagatt ggctcgctgg accgtgtcgc acgcaaccgg 600
attttgcatc tgacctcggc tcagaacaat gacggtgaca tggctgagct ggtgaaatcg 660
gtgagcgcgc ccgggctaca cgagcgcatt gcgcagtacc gcgccgggca ttga 714
<210> 140
<211> 801
<212> DNA
<213> Riemerella anatipestifer
<400> 140
atgtacaaat taatagatgt agataaccat tttgaaggaa agcttcaaat cgcatatatc 60
aatcagccag aatcgtttaa tagtcttaat aaggttgttt tagaagagtt attgcacttt 120
ataaaagctt gtgacgcaga ttctagtgta cgctgtattg caattagtgg caaaggtaag 180
gcgttttgtt ctggtcagaa tttaaaggag gctttagatt ataaagcaga agccaatgag 240
gaacgcttta tccaaaggat tgtgatagat tattataatc cgttagtgaa ggctattgtc 300
tatgctaaaa aaccagtaat tgcattggtt aatggtcctg cggttggtgc aggagcaatg 360
ttagctctca tctgtgattt tgcagtggcg tcagagtcag cgtatttttc cttagctttt 420
tctaatatag gactagtgcc agatacggca ggtacttact atttgcctaa acttttaggg 480
cgttccttag cgagttattt ggcatttaca gggaagaagc tatctgctaa agagtcttta 540
gaaagaggtt tggtggtaga tgttttttca gatgctactt tttcggaaca atctttacaa 600
gtcctagaac atattactca tcagcctact gtggcattgg ggcttacaaa aaaagccttt 660
aataaatctt atcagaatag tctatcggag cagttagatt tggagagtat tctccagcaa 720
gatgctgcag aaacttggga ttttcaagag gggatagccg cttttttagc aaaaagaaaa 780
cctcagtata aaggtaagta a 801
<210> 141
<211> 1269
<212> DNA
<213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007
<400> 141
atgtcagaaa caatcaattt agatgaaatg tcagcaaaac aattattggg ttattatcaa 60
gaaaaattgg atgaagaagc aagacaggca aaaagagaag gaaaattagt ttgttggtct 120
gcttccgttg ctccaccaga attctgtgta gctatggata ttgccatggt gtatccagaa 180
actcatgcag cagggattgg agctagaaaa gggtcgttag atctgctaga agtagcagat 240
gaaaaagggt attctttaga tatttgttct tatgcaagag taaatttggg gtatatggaa 300
ttgttaaaac aacaagcctt aactggagaa actcctgaaa aattagcaaa ctctccggct 360
gcaaaagttc ctttaccgga tttagttatt acatgtaata acatttgtaa tactttgtta 420
aaatggtacg aaaatttggc aaaggaatta aatattccat gtattgtaat tgacgttccg 480
ttcaatcata ctatgccaat tacaaaacat tcaaaagaat atattgcaga tcaatttaaa 540
tatgcaattc aacaattaga agaaattaca ggaaagaaat ttgactatga taaattctta 600
gaagtgcaag agcaaacaca aagatctgta tatcaatgga atcgtttagc agctcttgct 660
cactacaaac cttctccatt aaatggtttc gatttattta acttcatggc tttaattgta 720
tgtgctagaa gtagagatta tgcagaaatc actttcaaga aatttgcaga tgaattggaa 780
gaaaacttga aaaatgaagt atatgcgttc aaaggagctg aaaagaacag agttacttgg 840
gaaggaattg cagtatggcc ttaccttgga cacactttca agtctttaaa aggaatggga 900
agtatcatga ccggttctgc atatccagga atctggaact tgacatatac tcctggagat 960
atggaatcta tggcggaagc atatacaaga gtctacatta atacttgctt acaaaataaa 1020
gcggatgtcc tttctaaaat tgtaacagac ggaaaatgtg atggaatact atatcatttg 1080
aatagaagtt gtaaactgat gagtttcttg aatgtggaaa ctgctgaatt agttgaaaaa 1140
gcgactggag tgccatatgt aagtttcgat ggagaccaaa cagatccgag aaatttcgca 1200
ccggctcaat ttgatacaag agtacaagct ttaaatgaaa tgatggaagt taataacgaa 1260
acaaaataa 1269
<210> 142
<211> 834
<212> DNA
<213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007
<400> 142
gtgcaagatg acagaagttt taagaaagga aagagaagag gaatgtatac agttggagtg 60
gatataggtt cttcttcttc aaaagtagtg atattaaagg atggaacaga gattgtaagt 120
caatcggcaa ttcagtcggg aattggaagt aatcgagcca ttgttgcttt ggaagataat 180
ttaaaaaaag caaacttgac gaaggaagat attggtttta cagttgttac tggatatgga 240
cgctttactt ttgaaggagc agataaacaa atcagcgaga ttagttgtca tgccaggggg 300
attcattttt tattaccgaa tgtgagaacc attattgata ttggtggaca agatgccaaa 360
gcgatcagct tagatgaaaa aggtcatgta agacaatttt ttatgaatga caaatgtgca 420
gcaggaacag gacgattttt aactgtaatg gcacgcgtac tagagatttc cctagatgag 480
atgggaactt atgatgctct ttctaaaaat ccttgtaata ttagtagtac ttgtgctgta 540
tttgcagaat cagaagtcat ttctcaattg gcaaagggaa ataccaaaga ggatgtcatt 600
gcaggagtac ataattctgt cgctcataag atattaggtt tagtatatcg tacttctatg 660
gaagaaaaat ttgcgatttg tggtggtgtt gctcagaata caggtgcatt gcgtgcaata 720
cgggaagctt tgaaaaaaga agtaatcgtt gctcctaatc cacaattaac aggagcatta 780
ggagctgcaa tttttgctta tgatgagctg aaaaaattaa gaaagggtga ataa 834
<210> 143
<211> 1125
<212> DNA
<213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007
<400> 143
atgaaaggca gattagaaga attaattcat atatttgaag atgttgcaaa caaccccaaa 60
aaaatggtag cagaatataa aaaagaagta gggaaagaag tgattggagt catgccagta 120
tatgctccag aagaaattat tcacgctgct ggatgtttac ctattggatt atggggagga 180
aaaaaagaag tttctaaagc aagagcatat ttacctcctt ttgcatgttc tattatgcaa 240
actgttatgg aattacaaat tggaggaaca tatgacattt tagatgcagt attattctct 300
gtaccttgtg atactttgaa atgtttaagt caaaaatgga aaggaaaatc tcctgtaatt 360
gtatttactc atcctcaaaa cagagtaatt gaaggagcaa atgcttactt agtaaaggaa 420
tatcaagcag taaaagaaaa attagaagga atcttaggaa gaaccattcc tatggaagcg 480
attgaagaaa gcgtaaaagt atataatgaa aatagaagag ttatgagaga atttgtagaa 540
gtggcggcac aatatccaca aattatcgat ccaattgtta gacataatgt gatgaaatcc 600
agatggttct taagaaaaga aaaacatact gaatatgtaa aagaattaat cgctgaatta 660
aaaaaagaaa ctattgttcc ttgggacgga aagaaagtaa tcttaacagg aattatgaca 720
gaaccagtag aattgttgca aatctttaaa gatgaaaaac ttgctattgt agccgatgat 780
ttagctcatg aaagccgaca atttagagga gatgttcctg aagaaggagg agatgttcta 840
tacagaatgg caaaatggtg gcaaaattta gaaggatgtt ctttagcaac ggatactaat 900
aaaggtagag gacaaatgct aatggatatg tgtaaggata cgaaagcaga tgccgttatc 960
gtgtgtatga tgaaattctg tgatcctgaa gaatttgact atccggtata ctatagagaa 1020
tttactgaat ccggaattaa aaatattaca gtggaagtgg acttagaagt ttcttctttt 1080
gaacaaatta gaacaagaat acaaacattt aaagatattt tataa 1125
<210> 144
<211> 1269
<212> DNA
<213> Desulfosporosinus youngiae DSM 17734
<400> 144
atgacggata caacaactat gagtgccaaa gaattgttag gtttctatca ggaagaattg 60
tatgaagaag cgagacaggc caaaaaagaa ggaaaacttg tttgttggtc tgcatcggtt 120
gctccttcgg agttttgtgt ggctatggat gtggcgatga tctatcctga aacacatgct 180
gcggggattg gggcaagaaa aggtgcctta gatgtgctgg aagttgccga tgaaaaaggc 240
tataacctgg atacttgctc ctatgcaaga gtcaatatgg gttatatgga acttctgaaa 300
caagaggctt taacaggaat aacgccggaa aagcttgaaa aatccccggc ggccagaata 360
ccgctgcccg attttgtcat aacctgcaac aacatttgca acaccttgct taagtggtat 420
gagaatcttg ccgttgaatt aaatattccc tgcatcatca ttgatgttcc ctttaatcat 480
accatgccca ttccccagta tgctaaggac tatattgcgg aacagtttaa ggaggctatt 540
actcagcttg aggaaatttg cggcaggaaa ttcgactacg acaaattttt gaaagtacag 600
gaacaaaccc agcgttctgt ggcccagtgg aacagaattg ctgctttgtc gggacataaa 660
ccatctcctt taaatggttt tgatcttttc aactatatgg ccctgatcgt ttgtgccaga 720
agcagagact acgcggaaat tacctttaaa aagtttgccg atgaacttga agaaaacctc 780
aaaaacggta tctacgcctt taaaggaaat gaacaaaagc gtgtaacttg ggagggcata 840
gctgtttggc cgcatctggg ccatacattt aaaggcttaa agaatctggg caatatcatg 900
acaggttcgg cttatcccgg tttgtggaat cttacctaca cacctgggga tatgagttcc 960
atggcggaag cttataccag aatttatatc aatacttgtc tcgataacaa agttaaggtg 1020
cttagtgacg tcatcagcgg cggaaagtgt gacggggtta tttatcatca gaacagaagc 1080
tgtaagctca tgagtcttct caatgtcgaa acggctgata tactccaaaa acaaaatcat 1140
ttaccctatg tcagctttga tggggaccaa acggatcctc gtaactttgc tcctgcccag 1200
tttgatacac gtatccaggc cttagatgaa atgatgaagc agaataagga gggagtttcc 1260
aatgagtag 1269
<210> 145
<211> 1119
<212> DNA
<213> Desulfosporosinus youngiae DSM 17734
<400> 145
atgagtagaa ttgaaacgat tatcagtgaa ttaacgtcca ttgccaataa tccccgccag 60
gctatggaag attataaaaa agaaaccggc aaagggtcgg ttggggttat gccttattat 120
gctcctgaag aaatcattca tgccgcaggg tatctgcccg taggtatttg gggaggacaa 180
aagagtattt ccaaggcccg ggcctatttg cctccctttg cttgttcaat tatgcaatcc 240
gtggtggaaa tgcagcttga aggggtctat gacgatttag aagcggtcct tttccctgtt 300
ccttgtgaca ccttaaaatg tcttagccaa aaatggaaag gaacctcccc tgtcatcgtt 360
ttaactcatc ctcaaaacag aaaactggaa gcagccaata agtttcttgc tgaggaatat 420
aggcttgtgc gtgaaaaact ggaaaaaatc ctgaatgtta agattacaga cgaggcactt 480
aaccaaagca ttgaaattta taacgaaaat cgtaaagtaa tgcgtgaatt tacagagata 540
gctgctaatt atcccaacat tattgatccc gtaaaacgtc atgcgcttat caaagccaga 600
ttctttatgg aaaaagccaa acataccgct ctggtcaaag aattgaatgc agagcttaaa 660
gcgttaccgg tggaagcctt tacaggcaaa aaggttgttt tgacaggcat tatggctgaa 720
cccaatgaag tattggacat tttgcaagat aacggttttg ctgttgtggc agatgacctg 780
gcccaggaat ccagactgtt cagaaatgat gttccctcag ggacagaccc actctatcgc 840
ttggctaaat ggtggcagga attcgatggt tgttctctgg ctgtcgatgc gaaaaaacca 900
agaggcccca tgctgatgga tatggttaaa gcatctaagg ccgatgccgt tgtggtttgc 960
atgatgaagt tctgtgaccc tgaagaattt gactatccaa tctactacag acagtttgaa 1020
gaagccggaa ttaagagctt atttatagaa attgacctgg aaccaacctc ctttgaacag 1080
actaaaacca gagttcaaag ttttagagaa atgctgtga 1119
<210> 146
<211> 819
<212> DNA
<213> Desulfosporosinus youngiae DSM 17734
<400> 146
atgtttacaa tggggattga tattgggtcc tcatcctcaa aggttgtaat ccttgaagat 60
ggagttaata ttatcgccgg agaagttatt cagattggaa caggttctac gggacctaaa 120
cgtgtactgg atgaagctct tgccaaagca ggtcttacat tgcaagacat ggctaaaatt 180
attgctacag gctatggaag atcgtctgtg gaagaagcac acaaacaaat cagcgaaatc 240
agttgtcagg ctaagggagt tttcttttta gttccttcag caaaattaat tattgatatt 300
ggcggtcagg atgttaaggc cattaaactt gacagtaaag gctgtgttaa gcagtttttt 360
atgaatgata aatgtgccgc cggaacagga cgttttctcg atgttatgtc gcgggtactg 420
gaagttaatc ttgatgaaat ggcggaatac gatgcccggg caacagaacc tgccacggtc 480
agcagcactt gcacagtttt tgcagaatct gaggtaatat ctcagcttgc caacggagtt 540
gctaaagaga acattattgc aggggttcac cagtcagttg ctagcaaagc ctgtggactt 600
gcctatcgat gtggggtgga agaggacatc gtgatgtgcg gaggcgttgc taaggactta 660
ggggttgtca gagcaatcag caaagaactg aaaaaaccgg tcattgtagc tcctaatcca 720
caaattacag ctgcacttgg agctgctata tttgccttcg aagaagttat ggaaactgtt 780
atggttgcct tcgaagaagt taggggagct aataaataa 819
<210> 147
<211> 1269
<212> DNA
<213> Peptoniphilus indolicus ATCC 29427
<400> 147
atgaatacta tagatatatc aaatatgaaa gctaaagaaa tgcttggata ttttcaaaac 60
aaacttgacg aagaagcacg tgaagctaaa aaaaatggaa aattagtttg ctggtcagcc 120
tctgtagctc catctgaatt ttgtgtaacc atggatatcg cattagttta tccagaaact 180
cacgcagccg gtataggtgc tagaaaaggc tctttagcta tgttagatgt tgctgataga 240
aaaggttata atacagatat atgttcttat gccagagtaa acttaggata tatggaactt 300
ttaaaagaat atgctaagac aggagtgaaa cctaaagaac ttgaagaatc tcctgctgca 360
gatgttcctc tacctgattt agtaataact tgcaataata tatgcaacac tttactaaaa 420
tggtatgaaa atttagctgc agaattaaat attccttgta tagttataga cgttcctttt 480
aatcatacta tgcctattcc taagtattct aaagaatata ttgctgacca atttaaggaa 540
gcaataagac aacttgaaga aataacagga aaagattttg actatgataa atttttagaa 600
gttcaagagc aaacgcaaag atctgttgct caatggaata gacttgctgc actttctaaa 660
tatgaaccgt ctcctctaaa tggatttgat ttatttaact atatggctct tatagtttgt 720
gcaagaagta aaaattatgc tgaattaact tttaaaaaat ttgccgatga acttgaagaa 780
aatatgcaaa atggagtgta tccttacaag gctggagaac aatccagaat tacttgggaa 840
ggtatagcta tttggccata tttaggacac acttttaaga ctcttaaagg ctatggctca 900
ataatgacag gctctgctta tcctggactt tggaacttag aatacacacc tggagatatg 960
ctttcaatgg cagaagctta tacgagaata tatataaaca cttgccttga caataaagtt 1020
gatgtattga gaaaaatcat taaaaacggt aaatgtgatg gggtcgcata ccatctaaat 1080
agaagttgta aattgatgag tcttctaaac gttgagacag ctgaaatttt aaataaagaa 1140
aataatcttc catatgttag ttttgatggt gatcaaactg atcctagaaa tttctcagaa 1200
gcacaatatg ataacagaat acaaactctt actgagatga tgtctgccaa taaaaaaatg 1260
aggggttga 1269
<210> 148
<211> 792
<212> DNA
<213> Peptoniphilus indolicus ATCC 29427
<400> 148
atgtacacta tgggagtaga tatcggttct acatcatcta aaatcataat acttgaagat 60
ggaataaaaa ttatcggaaa tattgtagta caatctggaa ccggtacaag tgggccaaca 120
attgctactg caaaagctaa gtcctttctt tcaaataata atttaacttt agatgatata 180
tctaaaatcg ttgtcacagg ttacggcaga ttttcatttg atattgccga taaacaaata 240
agtgaaataa cttgtcatac aaaaggtatt aactttttag tgcctgaagc tcgaactatt 300
ttagatatag gtggacaaga tacaaaagct atttcagtta atgataaagg tcaagttcta 360
caatttttca tgaatgacaa atgtgccgcc ggcactggca gatttttaga agtcatggct 420
aaaattttag aaataccttt agaaaaaatg ggtgaatatg atagattatc aactaatccg 480
gtagctataa gtagtacttg taccgttttt gctgagtctg aagttatttc tcagctatca 540
aagggcatat ctaaagaaaa tatattagcc ggtgtacata attcaactgc taacaaagtt 600
tgtggtcttt tatatcgtac aggaattaag gaaaaaatag ttttatgtgg aggagttgct 660
caaaaccaag gtgttgttag agcgctccaa gaggaattaa aaaaagaaat aaccatagct 720
cctcacccac aaatgacagg cgccataggt gctgctttat ttgcttatga agaggcgaat 780
aaaaatttat ag 792
<210> 149
<211> 1119
<212> DNA
<213> Peptoniphilus indolicus ATCC 29427
<400> 149
atgaacaaaa ttaatgaaat aataaattta ttggatgaag tttctaaaga tcctaaacta 60
acagttaaaa aatataaaga aaaaacagga aaaggtgttg taggtgtcat gccattatat 120
gcacctgaag aaattattca tgctgcaggt tttctaccta tgggactttg gggtgcacaa 180
aaagaagtat ctaaagcaag aatttattta cctccttttg catgttcaat aatgcaaact 240
aatatggaac ttcaaataga aggtgcctat gatgacttag atgcagttgt attttctgta 300
ccgtgcgata ctctaaaatg tatgagtcaa aaatggaagg gtaaaagtcc tgttatagta 360
tttactcatc ctcaaaacag aaaattagaa tctgcaaata aatttttggt tacagaatat 420
gaaatcttaa aagataaatt agaaaagata ttaaatgtaa aaatatctga tgaatccata 480
acaaatagta ttgaaattta caatgaaaat agaaaagtca tgagagaatt ttcagaccta 540
gctggtcaat atcctaatat aattgaccct attcaaagac atattgtatt taagtccaga 600
tggtttatgg aaaaatcaga acatactaaa ttagttaaag aactaatatc tgaaattaaa 660
aaattaccta ttgaagaatg ggatggctat aaagttatag caactggtat tatgatagaa 720
cctgaagaaa tacttcaaat atttaaagat aagaaaatag ctattgttgc agatgattta 780
gctcaagaat caagacaatt tagacatgac gtacctgaag gagatcaacc tcttttaaga 840
cttgctaagt ggtggcaaaa tttagaagga tgtgctcttg caactgatac aaaaaaatta 900
agaggccaaa tgctaattga tatggcgaaa aaatataatg ccgatgctgt attgatatgt 960
atgatgaaat tctgcgatcc tgaagaattt gactaccctg tatactatag agagttccaa 1020
gaagctggca taaagaattt actaattgaa attgacttag aaatgacagc ttttgaacaa 1080
actaacacaa gacttcaaac tcttgtagaa actctctaa 1119
<210> 150
<211> 1269
<212> DNA
<213> Desulfosporosinus meridiei (strain ATCC BAA-275/DSM 3257/ NCIMB 13706/S10)
<400> 150
atgactgata caacagctat gagcgccaaa gaattgttag gtttctatca ggaagaattg 60
tatgaagaag cgagacgggc aaaaaaagaa ggaaaacttg tttgttggtc tgcatccgtt 120
gctccttcgg agttttgtgt ggctatggat gtagctatga tatatcctga aacccatgct 180
gcgggtattg gggccagaaa aggtgcctta gatgtgcttg aagttgcgga tgaaaaaggc 240
tataacgtgg atacttgctc ctatgcaaga gtaaatcttg gttatatgga acttttaaaa 300
caggaggctt taacaggaat aacaccggaa aagcttgaaa aatccccagc ggccagaata 360
ccccttcccg attttgtcat aacctgtaac aacatttgta acaccttgct taagtggtat 420
gagaatcttg ccgttgaatt aaatattcct tgcatcatca ttgatgttcc ctttaatcat 480
acaatgccca ttccacagta tgccaaggat tatattgcgg aacagtttaa ggaagctatt 540
actcagcttg aggaaatttg cggcaagaaa ttcgactatg acaaattttt aaaagtacag 600
gaacaaaccc aacgttctgt tgcccaatgg aatagaatcg ctgctttgtc atcacataaa 660
ccatcccctt taaatggttt tgatcttttc aactatatgg ccctgatcgt ttgtgcaagg 720
agtaaagact acgcagaaat tacctttaaa aagtttgctg atgaacttga agaaaatctt 780
aataagggta tcttcgcctt taaaggaaat gaacaaaagc gggtaacttg ggaaggcata 840
gctgtttggc cgcacctggg acatacattt aaaggcttaa agaatcttgg caatataatg 900
acaggttcag cctatccggg tctgtggaat gttagttata caccaggtga tatgagttca 960
atggcggaag cttatactag aatttatatc aatacttgtc ttgataataa agttaaggtt 1020
cttagtgacg taattagtgg cggaaagtgt gacggtgtta tttatcatca gaacagaagc 1080
tgtaagctca tgagttttct gaatgtagaa actgctgata tcctccaaaa agaaaatggt 1140
ttaccctatg taagctttga tggagaccaa actgatcctc gtaacttttc tcctgcccag 1200
tttgacacac gtatccaggc cttagatgaa atgatgaagc agaataagga gggagtttcc 1260
aatgagtag 1269
<210> 151
<211> 1119
<212> DNA
<213> Desulfosporosinus meridiei (strain ATCC BAA-275/DSM 13257/NCIMB13706/S10)
<400> 151
atgagtagaa ttgaaactat tattagtgaa ttatcttcaa tttcaaataa tccccgcaag 60
gctatggaag attataaaaa agaaaccggt aaagggtcgg taggggttat gccttattat 120
gcccctgaag aaataattca tgctgctggt tttcttcccg taggtatttg gggaggacaa 180
aagagtattt caaaagcccg tgcctattta cctccctttg cttgttcaat tatgcaatca 240
gttatggaaa tgcagcttga aggggtatat gacgatttag aagcagtact tttccccgtt 300
ccttgtgaca ctttaaaatg tctcagccaa aaatggaaag gaacatcacc tgtcatcgta 360
tttactcatc ctcaaaacag aaaactcgaa gcagccaata agtttcttgc tgaggaatat 420
cgacttgttc gtgaaaagct ggaaacaata ttgaatgtaa agattactga tgaagcactc 480
aaccaaagta ttgaaactta taacgaaaat cgtaaagtaa tgcgtgaatt tacggaccta 540
gctgctaatt atcctcagat tattgatccc agaatacgtc atgcaattat aaaagctaga 600
ttttttatgg aaaaatctaa acataccgct atggtaaaag aattgaattc agagcttaaa 660
tcgttacctg ttgaagcctt tacaggtaaa aaggttgttt taacaggaat tatggctgaa 720
cccaatgaag tattagacat tttaaaagat aacggttttg ctgttgtggc agacgacctg 780
gcccaggaat ccagactgtt cagaaatgat gttccgtcag gtacagaccc actatatcga 840
ttggctaaat ggtggcaaga attcgatggt tgttctcttg ctacagatgc gaaaaaatca 900
agaggcccca tgctgatgga gatggttaaa gggtctaagg ccgatgcagt tgtggtttgc 960
atgatgaagt tctgtgaccc tgaagaattt gactatccaa tctactatag acagtttgaa 1020
gaagctggaa ttaagagcct atttatagaa attgacctgg aaacaacatc ctttgaacag 1080
actaaaacca gagttcaaag ttttagtgaa atgctgtga 1119
<210> 152
<211> 786
<212> DNA
<213> Desulfosporosinus meridiei (strain ATCC BAA-275/DSM 13257/NCIMB 13706/S10)
<400> 152
atgtttacaa tggggattga tattgggtcc tcatcctcaa aggttgtaat acttgaagat 60
ggagttaata ttatcgctgg agaagtcatt cagattggaa caggttcgac aggacctaaa 120
cgtgtactga atgaagctct ttccaaagca ggtcttaaat tggaagacat ggctaaaatt 180
attgctacag gctacggaag atcttctgtg gaagaagcac acaaacaaat tagcgaaatc 240
agttgtcagg ctaagggagt tttcttttta gttccttcag caaaattaat tattgatatc 300
ggcggtcaag atgttaaggc aattagactt gacagtaaag gcggcgttaa gcagtttttt 360
atgaatgata aatgtgccgc cggaacagga cgttttctcg atgttatgtc acgagtactt 420
gaagttaatc ttgatgaaat ggcagaatac gatgctcgtg caacagaacc tgccacggtc 480
agcagcactt gcacagtttt tgcagaatct gaggtaatat ctcagctttc caacggagtt 540
gctaaagaga atattattgc aggggttcac cagtcagttg ctagcaaagc ctgtggactt 600
gcctatagat gtggggtgga agaggacatt gttatgtgcg gaggtgttgc taaggactta 660
ggggttgtcc gggcaataag caaagaacta aaaaaacctg tcattgtagc tcctaatcca 720
caaattacag ctgcccttgg agctgctatc tttgccttcg aagaagtcag gggagctaat 780
aaataa 786
<210> 153
<211> 1434
<212> DNA
<213> Acidaminococcus fermentans
<400> 153
atgccaaaga cagtaagccc tggcgttcag gcattgagag atgtagttga aaaggtttac 60
agagaactgc gggaaccgaa agaaagagga gaaaaagtag gctggtcctc ttccaagttc 120
ccctgcgaac tggctgaatc ttttcggctg catgttgggt atccggaaaa ccaggctgct 180
ggtatcgctg ccaaccgtga cggcgaagtg atgtgccagg ctgcagaaga tatcggttat 240
gacaacgata tctgcggcta tgcccgtatt tccctggctt atgctgccgg gttccggggt 300
gccaacaaaa tggacaaaga tggcaactat gtcatcaacc cccacagcgg caaacagatg 360
aaagatgcca atggcaaaaa ggtattcgac gcagatggca aacccgtaat cgatcccaag 420
accctgaaac cctttgccac caccgacaac atctatgaaa tcgctgctct gccggaaggg 480
gaagaaaaga cccgccgcca gaatgccctg cacaaatatc gtcagatgac catgcccatg 540
ccggacttcg tgctgtgctg caacaacatc tgcaactgca tgaccaaatg gtatgaagac 600
attgcccgtc ggcacaacat tcctttgatc atgatcgacg ttccttacaa cgaattcgac 660
catgtcaacg aagccaacgt gaaatacatc cggtcccagc tggatacggc catccgtcaa 720
atggaagaaa tcaccggcaa gaagttcgat gaagacaaat tcgaacagtg ctgccagaac 780
gccaaccgta ctgccaaagc atggctgaag gtttgcgact acctgcagta caaaccggct 840
ccgttcaacg ggttcgacct gttcaaccat atggctgacg tggttaccgc ccgtggccgt 900
gtggaagctg ctgaagcttt cgaactgctg gccaaggaac tggaacagca tgtgaaggaa 960
ggcaccacca ccgctccctt caaagaacag catcgtatca tgttcgaagg gatcccctgc 1020
tggccgaaac tgccgaacct gttcaaaccg ctgaaagcca acggcctgaa catcaccggc 1080
gttgtatatg ctcctgcttt cgggttcgtg tacaacaacc tggacgaatt ggtcaaagcc 1140
tactgcaaag ccccgaactc cgtcagcatc gaacagggtg ttgcctggcg tgaaggcctg 1200
atccgcgaca acaaggttga cggcgtactg gttcactaca accggtcctg caaaccctgg 1260
agcggctaca tgcctgaaat gcagcgtcgt ttcaccaaag acatgggtat ccccactgct 1320
ggattcgacg gtgaccaggc tgacccgaga aacttcaacg cggctcagta tgagacccgt 1380
gttcagggct tggtcgaagc catggaagca aatgatgaaa agaaggggaa ataa 1434
<210> 154
<211> 1140
<212> DNA
<213> Acidaminococcus fermentans
<400> 154
atggctatca gtgcacttat tgaagagttc caaaaagtat ctgccagccc gaagaccatg 60
ctggccaaat ataaagccca gggcaaaaaa gccatcggct gcctgccgta ctatgttccg 120
gaagaactgg tctatgctgc aggcatggtt cccatgggtg tatggggctg caatggcaaa 180
caggaagtcc gttccaagga atactgtgct tccttctact gcaccattgc ccagcagtct 240
ctggaaatgc tgctggacgg gaccctggat gggttggacg ggatcatcac tccggtactg 300
tgtgataccc tgcgtcccat gagccagaac ttcaaagtgg ccatgaaaga caagatgccg 360
gttattttcc tggctcatcc ccaggtccgt cagaatgccg ccggcaagca gttcacctat 420
gatgcctaca gcgaagtgaa aggccatctg gaagaaatct gcggccatga aatcaccaat 480
gatgccatcc tggatgccat caaagtgtac aacaagagcc gtgctgcccg ccgcgaattc 540
tgcaaactgg ccaacgaaca tcctgatctg atcccggctt ccgtacgggc caccgtactg 600
cgtgccgctt acttcatgct gaaggatgaa tacaccgaaa agctggaaga actgaacaag 660
gaactggcag ctgctcctgc cggcaagttc gacggccaca aagtggttgt ttccggcatc 720
atctacaaca cgcccggcat cctgaaagcc atggatgaca acaaactggc cattgctgct 780
gatgactgcg cttatgaaag ccgcagcttt gccgtggatg ctccggaaga tctggacaac 840
ggactgcatg ctctggctgt acagttctcc aaacagaaga acgatgttct gctgtacgat 900
cctgaatttg ccaagaatac ccgttctgaa cacgttggca atctggtaaa agaaagcggc 960
gcagaaggac tgatcgtgtt catgatgcag ttctgcgatc cggaagaaat ggaatatcct 1020
gatctgaaga aggctctgga tgcccaccac attcctcatg tgaagattgg tgtggaccag 1080
atgacccggg actttggtca ggcccagacc gctctggaag ctttcgcaga aagcctgtaa 1140
1140
<210> 155
<211> 783
<212> DNA
<213> Acidaminococcus fermentans
<400> 155
atgagtatct ataccttggg aatcgatgtt ggatctactg catccaagtg cattatcctg 60
aaagatggaa aagaaatcgt ggcgaaatcc ctggtagccg tggggaccgg aacttccggt 120
cccgcacggt ctatttcgga agtcctggaa aatgcccaca tgaaaaaaga agacatggcc 180
tttaccctgg ctaccggcta cggacgcaat tcgctggaag gcattgccga caagcagatg 240
agcgaactga gctgccatgc catgggcgcc agctttatct ggcccaacgt ccataccgtc 300
atcgatatcg gcgggcagga tgtgaaggtc atccatgtgg aaaacgggac catgaccaat 360
ttccagatga atgataaatg cgctgccggg actggccgtt tcctggatgt tatggccaat 420
atcctggaag tgaaggtttc cgacctggct gagctgggag ccaaatccac caaacgggtg 480
gctatcagct ccacctgtac tgtgtttgca gaaagtgaag tcatcagcca gctgtccaaa 540
ggaaccgaca agatcgacat cattgccggg atccatcgtt ctgtagccag ccgggtcatt 600
ggtcttgcca atcgggtggg gattgtgaaa gacgtggtca tgaccggcgg tgtagcccag 660
aactatggcg tgagaggagc cctggaagaa ggccttggcg tggaaatcaa gacgtctccc 720
ctggctcagt acaacggtgc cctgggtgcc gctctgtatg cgtataaaaa agcagccaaa 780
taa 783
<210> 156
<211> 1011
<212> DNA
<213> Carboxydothermus hydrogenoformans
<400> 156
atgaaattaa actatttttg cagttactgg ccggtggaaa tatccgaagg agcggggatt 60
tctacggtcc gttatttccc gtccgatgaa agcaaagctc cggtaaggct tcctgcttac 120
tgctgttctt atgccagggg aagccttgcc gaaattgaag aagaaggaga cggtgacttt 180
tggggatttg cccacagttg cgacacgatg cagagtttat acggcattac taagagttta 240
ctgggagacg accgggtttt tcttttcgtt ccgccggttg acttaaccac cgcttttgcc 300
cgggaatact accgggaagc tttaatttat ctctggcggg aactttccca aaaaagcggg 360
gttaatggtg aggaaaagtt aaagcttacc tgggaaaagt tgaaggagtt aagaaataag 420
gttaaatctt tggaaaactt gacgtcaatt attccttcct ccgaaatttt tgagctttta 480
aaaaagcttc agaccctgcc gctggatgag gctttggatt acctcgaggc caaaaaagcg 540
gaatttacca gtttatctgt ggctcaaaag gctataggga ttattttaac gggagcggta 600
gtcactaaca gtaaacttta ccttgcttta gaacaacagg gatttagagt agtttatgat 660
gatacctgta ccggctttcg tcattttgct ggagagatag aggataaaga cgatattttg 720
gaggcaatag tttcttacta cctttcaaag cccccctgtc cctgcaggca taagggagta 780
tgggcgaggg cggagtattt aaaaaatctt tatcataaca aaaatgcccg ggccattgta 840
cttttacaaa ataaattttg tgaccccttt gcctgggatg ttccctattt agtggactac 900
tttaaaaaac agggagttcc ggttttagtt ttagaggtgg aaggcggaga aatcggcgag 960
caaaataaaa ctcgcctcca ggccttccgg gaaagcgtgg gtggagtgta a 1011
<210> 157
<211> 1215
<212> DNA
<213> Carboxydothermus hydrogenoformans
<400> 157
atggctaaaa aaatctttaa gcctcttaag gcttcagaga aaataaataa aattttaaaa 60
aatcattatt taaaagcaaa gtatttgcca acgcttggaa aattttttgg ttataaaacc 120
gcctggatta ccagcggagc tccggtggaa ctactgcggg cctttggtat agagccggtt 180
tatccggaga attacggtgc catttgcggt gcccgcaagg tttcgccgag tctttgccag 240
gtagcggaaa acaggggtta ttctctcgat ttgtgttctt atgccaagag taatctcgga 300
agtatctgga atccgaaaga aagtccattt aacggcttac cccggccgga tttactggtg 360
gtttgcaaca acatttgcgg gacggtttta aagtggtacg aaactttaag ccgggaattt 420
aatattcccc tttttatcat tgatacccct tttatcaccg gtgaacccca accctggcaa 480
atccagtatg tggccaaaca gatagaaaaa ctggcgattg aactggaaaa atttttccgg 540
aaaaagttgg atttaaaccg tttggaaaaa gtaattctcc ttgccaatga gacggtggat 600
ttatggaagg ggataagaaa ttttgccaaa aataaacctt cgccggtaaa cgttaccgat 660
ttatttatta atctggggcc aatggtggtt ttaaggggta ccgaagttgc ccgggatttt 720
tacgaggaag tttaccggga agtggaagaa aggtacaaag ccggggttcc ggcggtagag 780
ggagaaaaat accgtttagt ctgggacaac attcccatct ggtacggact gtaccgtttt 840
tacggttatt ttgccgaaag gggagcggtt tttgttaccg attcctatac cggtggctgg 900
gcggtcaaca taaaaaaggg tcctcccttt tatgcattag ccgagaccta tgccggcgtc 960
tttttaaatc gggatttaga atttcgcaaa aatcagttgc aatctttcat tgaggaattt 1020
tctgccgatg gctttgtcat gcactccaat cgttcgtgca aagcttattc ttttgtgcag 1080
gaggaaatcc ggcgccaaat catgaggtca ctaggagtgc cggggttaat agtggatgcc 1140
gatatgaccg acagccggct ttattccgaa gaaacggttt taaaccgggt ccaggctttc 1200
ctggagagcc tgtag 1215
<210> 158
<211> 765
<212> DNA
<213> Carboxydothermus hydrogenoformans
<400> 158
ttgtatcttg gagttgatat tggttcgctt acgaccaagg ttgtcttaat tgaccgggga 60
aaaaatctta ttgcttatcg ttacagtaaa accggacctg ccggaaagga aacggccgag 120
cggttaattc aagaggtttt gataaaagcg aatatttccc gggacgatat tcagggaata 180
gttgctaccg gttacggcag ggttctcttt tccggaaagg agttttcgga gataacctgt 240
caggcccggg ggattgggca tttatacccg gaggcaaaaa cgattatcga tattggtggc 300
caggatagca aagtaatttc tctgggaaaa aacggaaagg tactggactt tgccatgaac 360
gataaatgtg ctgctggcac cggacgtttt ttggaggtga tgagtcaggc ccttgaagtt 420
cgtctggaag agatagggga acttgccgaa aagagccagg aggcagctaa gatatcttcg 480
gtttgtaccg tttttgccga atcggaagtg atatccaatt tatcccgggg gcagagccgg 540
gaagcggtag cacggggaat ttgtgaggcg gtggcggccc gaacggctat actggcgcaa 600
aaagtggggg tggtagaacc ggtggttttt accggagggg tggccaaaaa tactggagtt 660
gtggcggctt tggagcgaaa gcttggggtt aagttattaa ttccggaaga ttccacgatt 720
accgcagctc tgggggcggc tttattagcc gctgaaaatt cttaa 765
<210> 159
<211> 786
<212> DNA
<213> Oscillibacter valericigenes
<400> 159
atgaacaata tttacacgat gggcatcgac gtggggtcca ccgcatccaa gtgcctcatc 60
ctgaaagacg gcagcgaaat cgttgccaag tctctggtag atgtgggcgc gggtaccagc 120
ggccctaccc gtgctattgc ggaggtactg gaagccgcgg ggatgaagaa ggaggacatg 180
gcttttattc tggctaccgg ctatggccgc aattcactgg acgacattgc cgaccaccag 240
atgagcgagc tgagctgcca tgccaaaggc gcgtttttcc tgtttccgga tgtccacacc 300
gtcatcgaca tcggcgggca ggatgtgaag attcttgaga ttgagaacgg cgttatggtg 360
aattttgcca tgaatgacaa gtgcgccgcc gggacgggcc ggttcctgga cgtgatggcc 420
cgggtgctgg aggtgaaggt ggaggatctg gcggacctgg gagcccagtc caccaagaat 480
gtggagatca gctccacatg caccgtgttc gctgagagcg aggtcatcag ccagctggcc 540
aagggcagcg acaagcgcga catcatccac ggcatccaca agtctgtggc atcccgggtg 600
gttggccttg ccaaccgtat cggtgtgcgg gacgcggtgg tgatgaccgg cggcgtcgcc 660
cagaacggcg gcgtggtctc cgcgcttcag gaggcgttgg gccatcccat tcacacttcg 720
cctctgacgc agtacaacgg cgcgctgggc gcggcgttgt ttgcatggca gaaggcaacc 780
aaataa 786
<210> 160
<211> 1284
<212> DNA
<213> Oscillibacter valericigenes
<400> 160
atggccgaaa acgaaaaagc cactgcggcc gctcccgagg cggctcctgt taagaaagct 60
ccgaagccgg tcagccccgg tacgcaggcg ctgcgcgacg ttgtcaccaa ggtgtacgcc 120
gccgcgtggg atgcgaaaaa ggcgggccgc cccgtgggct ggtcgtcttc caagttcccc 180
tgcgagatcg ccgaggcgct gggccttgca gtcgtatatc cggaaaacca ggctgccggt 240
atcggcgccc agcacgatgg ccagcggatg tgtgaatctg ccgagtcctt gggcttcgac 300
ccagatatct gcggatacgc ccggatttcc ctggcttatt ccgcgggcgt tgagacgacc 360
aatgagtccc gccgggttcc catgccggac ttcgtgctgt gctgcaacaa tatttgtaac 420
tgcatgacca agtggtatga gaatattgcc cggatgcaca acattcccct gattatgatc 480
gacgtgccct ataacaacga ggtcaccgtc agcgattccc aggtggctta cattcgcggc 540
cagttcgatg acgccattaa gcagatggag aagattgccg gcgtgaagtt cgacgaaaag 600
aagtttgaac aggcctgcgc caatgccaac cgcactgcca aggcgtggct gacggtctgt 660
gactatttgc agtataagcc cgctcccatg agcggcttcg atctgtttaa ccatatggct 720
gatgtggtga ctgcccgcgg caaggtggag actgccgagg cgttcgagct gctggcaagc 780
gagctggaac agcacgtaaa aaacggaacc agcaccgctc cgttccccga gcagtaccgc 840
gtcatgttcg agggcattcc ctgctggccc aacctaagga cgcttttcaa gcccctgaaa 900
gccaacggcg tcaacgtcac cgccgtggtg tacgcgcccg cgttcggttt tgtgtataac 960
gggctggacg agatggcccg cgcatactgc aaggccccca acagcgtgtg cattgagcag 1020
ggcgtggact ggcgcgaggg catctgtcgc gagaacaagg tagacggcgt gctggtgcac 1080
tataaccgat cctgcaagcc ctggtccggc tacatggccg agatgcagcg ccgtttcacc 1140
aaggatctgg gcgtcccctg cgccgggttc gacggagatc aggccgatcc ccgcaacttc 1200
aacgaggctc agtatgagac ccgtgtccag ggcctggtag aggctatgga ggagaataaa 1260
aagcagaagg aggcccgggc atga 1284
<210> 161
<211> 1143
<212> DNA
<213> Oscillibacter valericigenes
<400> 161
atgagtatcg aaacgattgt aaaggagttt gccgacgttg cggccgaccc gaaagcacag 60
ctgaagaaat acaaggcgga gggcaaaaaa tgcattggtg tgatgccgta ttacgcgccc 120
gaggagctgg tggccgccgc cggtatggtg ccgtttggta tgtggggcag caatgacaag 180
accatttctc gcgccaagga atactgcgct acattttact gcaccatcgc ccagctggat 240
cttgagatgc tgctggacgg caccatggat cttttagacg gagtcatcac ccccaccatc 300
tgcgacacgc tccgtcccat gagccagaac atccgcgtgg ccatgggcga gaagctcccc 360
tgcattttcc tggcccatcc ccagaaccgc aagcccgctt acggcaagaa gttctgcctg 420
gaccaatata cccacatcaa gactgagctt gagaagatcg ccggcgcgcc catcaccgac 480
gccgcactgt ccgagaccat caaggtctat aataagagcc gcgccgcccg ccgtgagttc 540
gtgaagctgg tcagcgacca ctgcgatgtt atcaccccca ccaaacgcag cgctgttttg 600
aaagccgcgt ggtttatgcc caaggcggag tacaccgaga agctgaaggc cctcaacgca 660
gagctgaagg ctctgcctgt gtgcgactgg aaggggacca aggtggtcac ctccggcatc 720
atatgcgaca accctaagct tctggagatc ttcgaggaga acaaaatcgc catcgccgcc 780
gacgacgtgg ctcatgagtc ccgctccttc cgcgtagacg ctcccgagac cggcgatccc 840
atggaggcac tcgcccagca gtttgccaat caggattacg atgttctgct gtacgatgag 900
cattccagcg agaaccgccg gggcgagttt gtggccaagc tggtgaagga cagcggcgcc 960
aaggggctgg tcctgtttat gcagcagttc tgcgacccgg aggagatgga gtatccctcc 1020
ctcaaaaagg cgctggacga agccaagatc ccccacatca agctgggtgt ggatcaacag 1080
atgcgggact tcggtcaggc tcgcaccgcg attcaggcgt ttgccgatgt gatctccctc 1140
taa 1143
<210> 162
<211> 1269
<212> DNA
<213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628)
<400> 162
atgactgata cagccaatat gagtgctaaa gaattgttag gtttctatca ggaagaattg 60
tatgaagaag cgagacaggc caaaaaagaa ggaaaacttg tttgctggtc ggcttccgtt 120
gctccttcgg agttttgtgt agctatggac gtggccatga tctatcctga aacccatgct 180
gcagggatcg gggccagaaa aggcgcctta gatatgcttg aagttgccga tgaaaaaggg 240
tataacctgg acacttgctc ctatgccaga gtgaatctgg gttatatgga acttttaaaa 300
caagaggctt taaccggaat aaccccggag aaactggaaa aatctccggc ggccagagta 360
cccctgcctg attttgtcat aacctgcaac aacatttgta acaccttgct taagtggtat 420
gaaaatcttg ccgttgagct aaatattccc tgcatcgtca ttgatgttcc ctttaatcac 480
accatgccca ttccccagta tgctaaagac tatattgcgg aacagtttaa ggaggcaatt 540
gctcagcttg aagagatttg cggcaagaaa ttcgactatg acaaattctt gcaagtccag 600
gaacaaaccc agcgctctgt ggcccaatgg aaccggattg cttctttgtc agggcataaa 660
ccatccccct taaatggttt tgatcttttc aactatatgg ccctgatcgt ttgtgcccgc 720
agcagggact gcgcagaaat tacctttaaa aagtttgccg atgaactgga agacaatcta 780
agcaaaggaa tctacgcctt taaaggcaat gaacaaaagc gtatcacttg ggaaggcatc 840
gctgtttggc cgcacctggg ccataccttt aaaggcttaa agaatcttgg caatatcatg 900
accggttcag cctatcccgg tttgtggaat ctttcttata cgcccggtga tatgagttcc 960
atggcagaag cttacaccag aatttatatc aatacttgtc tggataacaa agttaaggtt 1020
cttagtgaca tcatcagcgg cggaaagtgt gacggtgtta tttatcatca gaacagaagc 1080
tgtaagctca tgagttttct caatgtcgaa acggccgata tcctccaaca acaaaatcat 1140
ttaccctatg tcagctttga tggagaccaa accgatcccc gtaactttgc tcctgcccag 1200
tttgatacac ggatccaagc cttagatgaa atgatgaagc agaataagga gggagtttcc 1260
catgagtag 1269
<210> 163
<211> 1119
<212> DNA
<213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628)
<400> 163
atgagtagaa ttgaagcgat tatcagtgaa ttatcttcta ttgccaataa tccccgtaag 60
gccatggaag attataagaa agaaacgggc aaagggtcgg tagggattat gccttattat 120
gctccggaag aaatcgttca tgccgccggt tacctgcccg taggaatttg gggagggcaa 180
aagagtattt ctaaagcccg tgcttattta cctccttttg cttgttcaat catgcaatcc 240
gttgtggaaa tgcagctgga aggggtctat aacgacttag cggcggtcct tttccccgtt 300
ccttgtgaca ctttaaaatg tctcagccaa aaatggaaag gcacatcccc ggtcatcgtc 360
atgactcatc ctcaaaaccg aaaactcgaa gcagccaata agtttctggc tgaggaatat 420
cgccttgttc gtgaaaagct ggaaaaaatc ttaaatgttc agattaccga tgaggcactg 480
aaccacagca ttgatgttta taacgaaaat cgcaaggcaa tgcgtgaatt tacggacata 540
gccgctaatt atttgaacat tattgatccc agaaagcgtc atgagattat caaggccaga 600
ttctttatgg aaaaatccaa acataccgcc ttggtcaaag aattgaattc cgagcttaaa 660
tctttacctg tggaagattt tacaggcaaa aaggtgattt taaccggaat catggctgaa 720
cccaatgaag tattagacat tttgaaagag aatgattttg ctgttgtggc agatgacctg 780
gcccaggaat ccagactgtt caggattgat gttccggctg gtccagaccc actctaccgc 840
ttggctaaat ggtggcaaga attcgacggt tgttctctgg ctgtagatac gaaaaaatta 900
agaggaccca tgctgatgaa tatggttaac gtggataagg ccgatgccgt ggtggtttgc 960
atgatgaagt tctgtgaccc tgaagaattt gactatccca tctactacag acagtttgaa 1020
gaagccggaa ttaagagctt atttatagaa attgacctgg agccaacctc ctttgaacag 1080
actaaaacca gagttcaaag ttttcgtgaa atgctgtga 1119
<210> 164
<211> 801
<212> DNA
<213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628)
<400> 164
atgtatacta tggggattga tatcggttcc tcatcctcaa aggttgtcat acttgaagat 60
ggagttaacc tcatcgccgg cgaagtcatt cagattggaa caggctcgac aggtcctaaa 120
cgggtactgg aggaagctct tgccaaaaca ggtctcacct tggcagacat ggctaaaatt 180
attgctaccg gctacggccg atcttctgtg gaagtatccg acaagcaaat cagcgaaatc 240
agctgtcagg ctaagggagt ttacttttta gttcctacag caaaattaat cattgatatc 300
ggcggtcagg atgtgaaggc cattagactt gaccgtatag gcggcgtcag gcagtttttt 360
atgaatgata aatgtgccgc cggaacagga cgttttctcg atgtgatgtc acgagtactg 420
gaagtggatc tggatgaaat ggcagaatac gatgcccggg ccacagaacc cgccacggtc 480
agcagcacct gcacagtgtt tgccgaatcc gaggtaatat ctcagcttgc caacggagtt 540
gctaaagaga atattattgc cggggttcac cagtccgttg ccagcaaagc ctgtggactc 600
gcctatcgat gcggggtgga agaggacgtt gtgatgtgcg gaggagttgc taaggactta 660
ggagttgtcc gggccatcag caaagaacta aaaaaaccgg tcattgtagc tcctaatccc 720
caaattacag ccgcccttgg cgctgcccta tttgcttatg aagaagttat ggaagctaat 780
aaattaagga aagaggtatg a 801
<210> 165
<211> 1236
<212> DNA
<213> Peptostreptococcus anaerobius CAG:621
<400> 165
atgagtaaca caggtgcagt tgaagaaaag ccggcaaaag tattgttagg cgagatagtt 60
gcaaaacatt ataaggaagc ttgggaagct aaagaaagag gcgaaaaagt tggttggtgt 120
gcttctaact tcccacagga aatatttgaa acaatggata tcaaggttgt attccctgaa 180
aaccaggcag cagcaatttc tgctaagggt ggtggacaga ggatgtgcga aatcgcagaa 240
aacgaaggat attcaaacga catatgtgct tacgctagaa tatctctagc atacatggac 300
gttaaagatg ctccagagtt aaatatgcct cagccagact ttgttgcatg ctgtaacaat 360
atctgtaact gtatgatcaa gtggtatgaa aatatagcta aagaactaaa tatacctcta 420
atccttgttg acgtgccata taacaatgac tatgaagcag gcgatgacag agtagaatac 480
ttaagaggac agttcgatca cgctataaag cagttagaag acttaactgg taaaaagtgg 540
gatgaaaaga agttcgaaga agtaatggca atatctcaga gaacaggtag agcttggtta 600
aaggctactg gatatgctaa gtacactcca tcaccattct caggatttga cgtattcaac 660
catatggcag ttgctgtatg tgctagaggt aaggaagaat cagcaatagc atttgaaaag 720
ctagctgaag aatttgatga aaatgtaaag actggtaagt ctacattcaa gggagaagaa 780
aagtacagag tactatttga aggtatagct tgttggccac acctaagaca tacatttaag 840
cagctaaagg attcaggagt aaacgtttgt ggtactgttt atgcagatgc attcggatac 900
atctacgaca atacttatga attaatgcag gcttattgtg gaactcctaa tgcaatatct 960
tatgaaagat cattagatat gagacttaag gttatagaag aaaataatat agacggtatg 1020
ttgatacata taaacagaag ctgtaagcag tggtctggta tcatgtacga aatggaaaga 1080
gaaataagag aaagaactgg tataccaaca gctacattcg atggtgatca ggctgaccca 1140
agaaacttct cagaagcaca gtacgacaca agagtacagg gtctaataga agttatggaa 1200
gcaaacaaag ctgcaaagat gaaggaggaa aactag 1236
<210> 166
<211> 1119
<212> DNA
<213> Megasphaera elsdenii DSM 20460
<400> 166
atgagtcaga tcgacgaact tatcagcaaa ttacaggaag tatccaacca tccccagaag 60
acggttttga attataaaaa acagggtaaa ggcctcgtag gcatgatgcc ctactacgct 120
ccggaagaaa tcgtatatgc tgcaggctac ctcccggtag gcatgttcgg ttcccagaac 180
ccgcagatct ccgcagctcg tacgtacctt cctccgttcg cttgctcctt gatgcaggct 240
gacatggaac tccagctcaa cggcacctat gactgcctcg acgctgttat cttctccgtt 300
ccttgcgaca ctctccgctg catgagccag aaatggcacg gcaaagctcc ggtcatcgtc 360
ttcacacagc cgcagaaccg taagatccgc ccggctgtcg atttcctcaa agctgaatac 420
gaacatgtcc gtacggaatt ggaacgtatc ctcaacgtaa aaatctccga cctggctatc 480
caggaagcta tcaaagtata taacgaaaac cgtcaggtta tgcgtgaatt ctgcgacgta 540
gctgctcagt acccgcagat cttcactccg gtaaaacgtc atgacgtcat caaagcccgc 600
tggttcatgg acaaagctga acacaccgct ttggtccgcg aactcatcga cgctgtcaag 660
aaagaaccgg tacagccgtg gaatggcaaa aaagtcatcc tctccggtat catggcagaa 720
ccggatgaat tcctcgatat cttcagcgaa ttcaacatcg ctgtcgtcgc tgacgacctc 780
gctcaggaat cccgccagtt ccgtacagac gtaccgtccg gcatcgatcc cctcgaacag 840
ctcgctcagc agtggcagga cttcgatggc tgcccgctcg ctttgaacga agacaaaccg 900
cgtggccaga tgctcatcga catgactaag aaatacaatg ctgacgccgt cgtcatctgc 960
atgatgcgtt tctgcgatcc tgaagaattc gactatccga tttacaaacc ggaatttgaa 1020
gctgctggcg ttcgttacac ggtcctcgac ctcgacatcg aatctccgtc cctcgaacag 1080
ctccgcaccc gtatccaggc tttctcggaa atcctctaa 1119
<210> 167
<211> 1119
<212> DNA
<213> Peptostreptococcus anaerobius CAG:621
<400> 167
atgagtaact tagaagaact atttggaaaa cttgctgtat gtccattaga gcagatagat 60
aaatatgttg ctgatggtaa gaaagttatt ggttgcgcgc cagtatatgc tccagaagaa 120
cttgtatacg catcaggtat gattcctatg gcaatatggg gagcagaggg tgaagtaact 180
cttgcaaaag aatatttccc agctttctac gtatcaatca tcttaagact tttagatcta 240
ggtctagaag gcaagcttga taagatgtca ggaatgattc taccaggtct aagtgacgga 300
ctaaagggac ttagccagaa ctggaaaaga gctgtaaaga atgttccagc attatatata 360
ggatatggac agaacagaaa gatagaagct ggtatagttt acaatgctag acagtatgaa 420
aagctaaaag tacagttaga agaaatagct ggaaagaaga tagaagatgc tcagatagaa 480
gaagcaatcg ttttatacaa caagcacaga aaagctatgc aggcattctc agaccttgca 540
gctaaacact taaatacagt tactcctagc ctaagagcta aggtaatgtc aagtgcatgc 600
ctaatggaca aggctgaaca tttagaaata gtagaagcaa tcaacgctga actttcagct 660
atgccagaag aaaaatttga tggtaagaag attgtaacta ctggactact agctaacagt 720
cctgaaatat taaagatatt tgaagaattt aaacttggta ttgttgctga caacataaac 780
cacgaatcag gacagtttga ttatttagtt gatgaagcta ctggtaaccc aataaaggcg 840
ttgtctaagt ggatttcaga tattgaagga agtactttgc tatacgatcc agaaaaacta 900
agaggacaga taatcatcga taaggctaaa aaatacgatg cagatggtgt agtataccta 960
ctatctaaat tctctgattc agatgaattt gactacccaa tcattagaaa acagctagaa 1020
gaggctggat atatgcacat cttagttgaa gtagatcagc aaatgactaa cttcgaacaa 1080
gcaaaaactg cattgcagac ttttgcagac atgatatag 1119
<210> 168
<211> 792
<212> DNA
<213> Peptostreptococcus anaerobius CAG:621
<400> 168
atgagtgata tatacacaat gggtattgac attggatcaa catcatctaa atgtgtagtg 60
cttaagaatg gtaaagattt agttagtagc ggcgtcgtca atcttggcgc cggtactaaa 120
ggtgccgatc aggttataga aaaggtacta gctgactgtg gtatcaagtt cgaagatctg 180
aatgtgattg tttccacagg atatggtaga aattcttacg acagtgcaaa gaagactatg 240
agtgaactta gctgtcatgc taagggtggt acatatatct tcggacctgt aagaactatt 300
atagatatag gcggacagga cataaaggta ctaaaactaa atgacaaagg tatgatgaca 360
aatttcttga tgaatgataa atgtgcagct ggtacaggta gattcttaga ggttatggct 420
ggagtacttg atgttaagct agcagaacta ggtgacttag acaagttagc aactgaaaaa 480
acaccaatat cttcaacttg tacagtattt gcagaatcag aagtaatatc ttgtatggct 540
aagaaaatac ctattcctaa tataattagg ggtatacacg cttctgttgc tacaagagtt 600
gcaggtcttg ctaagagagg tggattaaca actccagtcg ctatgactgg tggtgttact 660
aagaactcag gaatagtaag ggcacttagc gaagagttag aaacagatat catgatttcg 720
gaaatttctc agttggcagg cgcaattgga gcggcattgt acgcttacga tgagtatctg 780
aaggaaaatt ag 792
<210> 169
<211> 777
<212> DNA
<213> Chloroflexus aggregans (strain MD-66 / DSM 9485)
<400> 169
atgagcgatg aaacgcttgt gctcagcact atcgaaggcc ccgttgcaat ccttacgctc 60
aatcgaccac aagcactcaa tgcccttagc cctgccctca tcgacgcact catccgccat 120
cttgagcatt gcgataacga cgatacgatc cgggtgatca ttatcaccgg cgccggtcgc 180
gcctttgccg ccggcgccga catcaaggcg atggccgatg cgacgccgat cgatatgctt 240
acaaccgata tgattgcccg ctgggcgcgg attgcggcgg tgcgcaaacc cgtgatcgca 300
gccgtgaacg gatttgccct cggtggtggc tgcgagttgg ctatgatgtg tgacatcatt 360
cttgccagtg aaacagccca attcggtcaa cccgaaatca acatcggcat tatccccggc 420
gccggtggca cccaacgcct gacccgcgca attggcccat accgtgcaat ggagatggtc 480
ttaaccggtg ctaccatcag tgcccaagaa gcttacgcct acggcctggt gaatcgggta 540
tgcccacccg atagcctgct tgatgaagcc cgccggttgg cccagaccat tgcagccaag 600
ccgccgctcg ctgtgcgttt agccaaggaa gccgtgcgcg ctgcggctga aacgaccgtg 660
cgtgaagggt tagccattga attgcgtaac ttttatctgc tctttgccag tgccgatcag 720
aaagagggca tgcgagcctt tatcgaaaag cgtacagcca acttcagtgg tcgctaa 777
<210> 170
<211> 774
<212> DNA
<213> Marivirga tractuosa (strain ATCC 23168/DSM 4126/NBRC 15989/NCIMB 1408/VKM B-1430/H-43)
<400> 170
atggaattca taaaagtaaa cacacaatat aaaaagcata ttgcgctcat caatcttaac 60
agacctaaag aattaaatgc cttgaactta cagttaatga ctgaattgaa ggacacttta 120
aaggtcttgg atgaggatga aaatgttaga gttataattt taacaggtaa tgagaaggct 180
tttgccgctg gagcagacat taagcaaatg gcaggtaaaa cggctattga catgctcaat 240
gttgatcaat tcagcacttg ggatcaaatc aaaaaaacaa agaagccatt gattgcagcc 300
gtttcaggat ttgcattggg cggtggttgc gaattagcga tgacttgcga tatgattgta 360
gcgtcagaat ctgctaaatt cggtcagcct gaaataaaaa tcggagtaat gccgggagca 420
ggtggtacac aaaggttaac tagggcaatt ggtaaagcca aagcgatgga attagtcttg 480
actggtaatt ttattagtgc agaggaagca atgcattatg gcttagttaa taaagttgtt 540
cctacagaga tgtatctgga agcagctgct gaactggctg agcaaatagc acaaatgtct 600
cctgtagcag ctaagttggc aaaagaatca gttaacaggg cttttgaaac gcatttggac 660
gaaggcttgc actttgagag aaaaaacttc tatttaacat ttgcttcaga agatcagact 720
gaaggtatgg aagcttttgt agagaaaaga aagcctgaat tcaaggggaa ataa 774
<210> 171
<211> 774
<212> DNA
<213> Marinithermus hydrothermalis (strain DSM 14884 / JCM 11576 / T1)
<400> 171
atgtacgaga acctcatcgt ggagacgctc gagggcggcg tggggctcat tcgcatccac 60
cggcccaagc gcctcaacgc cctgaaccag gccaccatgg acgagatcgt ccgcgcagta 120
cgcgcgtttg aagcggatga cgcggtgcgc gcgatcgtcc tcacggggga cgagcgggcg 180
ttcgccgcgg gcgcggacgt caccgagatg gacggcgcga acgtgccgga gatgctctcc 240
gggtaccgct tcgagcagtg ggagaccctc cggcgcacca cgaaaccctt gatcgccgcg 300
gtctcggggt tcgcgctcgg gggcgggctc gagctcgcga tgctgtgcga catcatcgta 360
gcctcggaga ccgcgcggct cggccagccc gagatcaacc tcgggatcat gccgggggcg 420
ggcggcacgc aacggctcac gcggcaggtg ggcaagtacc tcgcgatgga gatggtcctc 480
acggggcgca tgctcaccgc ggaggaggcg taccgtcacg gcctggtgaa ccgggtcgtc 540
ccggtcgagt tctacctgga ggaagccatc cagatcgcgc gggagatcgc gaagaaagcc 600
ccggtggcgg tgcgcctggc caaggacgcg atcctcaagg cagaggacac gccgctcgag 660
gtgggcctcg cgtacgagcg ccacaacttc tacctgctct tcggcaccga ggacaagcaa 720
gaagggatcc gcgctttcct cgagaagcgc aagcccgaat ggaaagggag gtag 774
<210> 172
<211> 780
<212> DNA
<213> Chitinophaga pinensis (strain ATCC 43595 / DSM 2588 / NCIB 11800 / UQM 2034)
<400> 172
atgcaaccac aatttataat catacaccgg caggtagccc catatgtggc tcatatacag 60
ttaaaccgcc ccaaagaact caatgcactg aaccttgaac tgatgattga gctcagggat 120
gcattaaaaa tgttggatgc ggatgacaat gttcgtgcaa tcgtcatcag cggtaatgaa 180
aaagcattcg ctgcaggcgc ggatatcaaa cagatggcgg ggaaaactgc catggacatg 240
tataacattg accagttcag cacctgggac acaataaaaa aaactaaaaa gccgttgatt 300
gcggcagtaa gcggcttcgc gctgggaggg ggatgtgagc tggtgatgct atgcgatatg 360
atagtagcca gtgaaacagc gcggttcgga cagccggaaa taaaaattgg cgtcatgcct 420
ggcgcaggtg gtacacaacg cctgacccgc gccgtaggta aagccctggc catggaaatg 480
gtattgacag gtcgctttat cactgcacaa gaagctgcac gtgcaggtct tatcaaccgg 540
gtaataccgg tggaactttt cctgcaggaa gccatccggc tggcgactga agtagctgcg 600
cttagtccgt tggcagtaaa gatggctaaa gaatctgtac tgaaagcatt tgatagctcc 660
ctcgaagaag gactacattt tgaacgtaaa aacttttatc tgctgtttgc ctctgaagat 720
cagaaagaag gcatgcaggc ttttgttgat aagagatcac ctgtttttaa aggaaaataa 780
780
<210> 173
<211> 777
<212> DNA
<213> Megasphaera elsdenii DSM 20460
<400> 173
gtgtatactc tcggaatcga cgttggttct tcttcttcca aggcagtcat cctggaagat 60
ggcaagaaga tcgtcgccca tgccgtcgtt gaaatcggca ccggttcgac cggtccggaa 120
cgcgtcctgg acgaagtctt caaagatacc aacttaaaaa ttgaagacat ggcgaacatc 180
atcgccacag gctatggccg tttcaatgtc gactgcgcca aaggcgaagt cagcgaaatc 240
acgtgccatg ccaaaggggc cctctttgaa tgccccggta cgacgaccat cctcgatatc 300
ggcggtcagg acgtcaagtc catcaaattg aatggccagg gcctggtcat gcagtttgcc 360
atgaacgaca aatgcgccgc tggtacgggc cgtttcctcg acgtcatgtc gaaggtactg 420
gaaatcccca tgtctgaaat gggggactgg tacttcaaat cgaagcatcc cgctgccgtc 480
agcagtacct gcacggtttt tgctgaatcg gaagtcattt cccttctttc caagaatgtc 540
ccgaaagaag atatcgtagc cggtgtccat cagtccatcg ccgccaaagc ctgcgctctc 600
gtgcgccgcg tcggtgtcgg tgaagacctg accatgaccg gcggtggctc ccgcgatccc 660
ggcgtcgtcg atgccgtatc gaaagaatta ggtattcctg tcagagtcgc tctgcatccc 720
caagcggtgg gtgctctcgg agctgctttg attgcttatg ataaaatcaa gaaataa 777
<210> 174
<211> 1287
<212> DNA
<213> Megasphaera elsdenii DSM 20460
<400> 174
atgagtgaag aaaaaacagt agatattgaa agcatgagct ccaaggaagc ccttggttac 60
ttcttgccga aagtcgatga agacgcacgt aaagcgaaaa aagaaggccg cctcgtttgc 120
tggtccgctt ctgtcgctcc tccggaattc tgcacggcta tggacatcgc catcgtctat 180
ccggaaactc acgcagctgg tatcggtgcc cgtcacggtg ctccggccat gctcgaagtt 240
gctgaaaaca aaggttacaa ccaggacatc tgttcctact gccgcgtcaa catgggctac 300
atggaactcc tcaaacagca ggctctgaca ggcgaaacgc cggaagtcct caaaaactcc 360
ccggcttctc cgattcccct tccggatgtt gtcctcactt gcaacaacat ctgcaatacc 420
ttgctcaaat ggtatgaaaa cttggctaaa gaattgaacg tacctctcat caacatcgac 480
gtaccgttca accatgaatt ccctgttacg aaacacgcta aacagtacat cgtcggcgaa 540
ttcaaacatg ctatcaaaca gctcgaagac ctttgcggcc gtcccttcga ctatgacaaa 600
ttcttcgaag tacagaaaca gacacagcgc tccatcgctg cctggaacaa aatcgctacg 660
tacttccagt acaaaccgtc gccgctcaac ggcttcgacc tcttcaacta catgggcctc 720
gccgttgctg cccgctcctt gaactactcg gaaatcacgt tcaacaaatt cctcaaagaa 780
ttggacgaaa aagtagctaa taagaaatgg gctttcggtg aaaacgaaaa atcccgtgtt 840
acttgggaag gtatcgctgt ctggatcgct ctcggccaca ccttcaaaga actcaaaggt 900
cagggcgctc tcatgactgg ttccgcttat cctggcatgt gggacgtttc ctacgaaccg 960
ggcgacctcg aatccatggc agaagcttat tcccgtacat acatcaactg ctgcctcgaa 1020
cagcgcggtg ctgttcttga aaaagttgtc cgcgatggca aatgcgacgg cttgatcatg 1080
caccagaacc gttcctgcaa gaacatgagc ctcctcaaca acgaaggcgg ccagcgcatc 1140
cagaagaacc tcggcgtacc gtacgtcatc ttcgacggcg accagaccga tgctcgtaac 1200
ttctcggaag cacagttcga tacccgcgta gaagctttgg cagaaatgat ggcagacaaa 1260
aaagccaatg aaggaggaaa ccactaa 1287
<210> 175
<211> 1119
<212> DNA
<213> Megasphaera elsdenii DSM 20460
<400> 175
atgagtcaga tcgacgaact tatcagcaaa ttacaggaag tatccaacca tccccagaag 60
acggttttga attataaaaa acagggtaaa ggcctcgtag gcatgatgcc ctactacgct 120
ccggaagaaa tcgtatatgc tgcaggctac ctcccggtag gcatgttcgg ttcccagaac 180
ccgcagatct ccgcagctcg tacgtacctt cctccgttcg cttgctcctt gatgcaggct 240
gacatggaac tccagctcaa cggcacctat gactgcctcg acgctgttat cttctccgtt 300
ccttgcgaca ctctccgctg catgagccag aaatggcacg gcaaagctcc ggtcatcgtc 360
ttcacacagc cgcagaaccg taagatccgc ccggctgtcg atttcctcaa agctgaatac 420
gaacatgtcc gtacggaatt ggaacgtatc ctcaacgtaa aaatctccga cctggctatc 480
caggaagcta tcaaagtata taacgaaaac cgtcaggtta tgcgtgaatt ctgcgacgta 540
gctgctcagt acccgcagat cttcactccg gtaaaacgtc atgacgtcat caaagcccgc 600
tggttcatgg acaaagctga acacaccgct ttggtccgcg aactcatcga cgctgtcaag 660
aaagaaccgg tacagccgtg gaatggcaaa aaagtcatcc tctccggtat catggcagaa 720
ccggatgaat tcctcgatat cttcagcgaa ttcaacatcg ctgtcgtcgc tgacgacctc 780
gctcaggaat cccgccagtt ccgtacagac gtaccgtccg gcatcgatcc cctcgaacag 840
ctcgctcagc agtggcagga cttcgatggc tgcccgctcg ctttgaacga agacaaaccg 900
cgtggccaga tgctcatcga catgactaag aaatacaatg ctgacgccgt cgtcatctgc 960
atgatgcgtt tctgcgatcc tgaagaattc gactatccga tttacaaacc ggaatttgaa 1020
gctgctggcg ttcgttacac ggtcctcgac ctcgacatcg aatctccgtc cctcgaacag 1080
ctccgcaccc gtatccaggc tttctcggaa atcctctaa 1119
<210> 176
<211> 777
<212> DNA
<213> Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl)
<400> 176
atgagtgaag agtctctggt tctcagcaca attgaaggcc ccatcgccat cctcaccctc 60
aatcgccccc aggccctcaa tgcgctcagt ccggccttga ttgatgacct cattcgccat 120
ttagaagcct gcgatgccga tgacacaatc cgcgtgatca ttatcaccgg cgccggacgg 180
gcatttgctg ccggcgctga catcaaagcg atggccaatg ccacgcctat tgatatgctc 240
accagtggca tgattgcgcg ctgggcacgc atcgccgcgg tgcgcaaacc ggtgattgct 300
gccgtgaatg ggtatgcgct cggtggtggt tgtgaattgg caatgatgtg cgacatcatc 360
atcgccagtg aaaacgcgca gttcggacaa ccggaaatca atctgggcat cattcccggt 420
gctggtggca cccaacggct gacccgcgcc cttggcccgt atcgcgcaat ggaattgatc 480
ctgaccggcg cgaccatcag tgctcaggaa gctctcgccc acggcctggt gtgccgggtc 540
tgcccgcctg aaagcctgct cgatgaagcc cgtcggatcg cgcaaaccat tgccaccaaa 600
tcaccactgg ctgtacagtt ggcgaaagag gcagtccgta tggccgccga aaccactgtg 660
cgcgaggggt tggctatcga gctgcgtaac ttctatctgc tgtttgccag tgctgaccaa 720
aaagagggga tgcaggcatt tatcgagaaa cgcgctccca acttcagtgg tcgttga 777
<210> 177
<211> 132
<212> PRT
<213> E. coli
<400> 177
Met Ser Thr Thr His Asn Val Pro Gln Gly Asp Leu Val Leu Arg Thr
1 5 10 15
Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly
20 25 30
Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Leu Ala Lys Glu
35 40 45
Ile Ala His Gly Arg Val Val Thr Val Arg Val Glu Gly Met Thr Phe
50 55 60
Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys
65 70 75 80
Val Gln Lys Gly Thr Thr Ser Val Ser Ile Asn Ile Glu Val Trp Val
85 90 95
Lys Lys Val Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu
100 105 110
Ala Leu Phe Lys Tyr Val Ala Val Asp Pro Glu Gly Lys Pro Arg Ala
115 120 125
Leu Pro Val Glu
130
<210> 178
<211> 132
<212> PRT
<213> Klebsiella oxytoca 10-5245
<400> 178
Met Thr Thr Thr Asp Leu Ala Pro Lys Gly Glu Leu Val Leu Arg Thr
1 5 10 15
Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly
20 25 30
Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Met Ala Lys Glu
35 40 45
Ile Ala His Gly Arg Val Val Thr Val Arg Val Asp Gly Met Thr Phe
50 55 60
Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Asn Cys
65 70 75 80
Val Lys Arg Gly Asn Thr Ser Ile Thr Ile Asn Met Glu Val Trp Val
85 90 95
Lys Lys Val Ser Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu
100 105 110
Ala Leu Phe Ile Tyr Val Ala Val Asp Asn Gln Gly Lys Pro Arg Ala
115 120 125
Leu Pro Thr Leu
130
<210> 179
<211> 133
<212> PRT
<213> Cronobacter turicensis
<400> 179
Met Thr Thr Glu Gln Thr Thr Pro Gln Gly Glu Leu Val Leu Arg Thr
1 5 10 15
Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly
20 25 30
Trp Leu Met Ala Gln Met Asp Ile Gly Gly Ala Ile Leu Ala Lys Glu
35 40 45
Ile Ala His Gly Arg Val Val Thr Val Arg Val Asp Gly Met Thr Phe
50 55 60
Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys
65 70 75 80
Val Lys Arg Gly Asn Thr Ser Val Thr Ile Asn Ile Glu Val Trp Val
85 90 95
Lys Lys Val Ser Ser Glu Pro Leu Gly Gln Arg Tyr Arg Ala Thr Glu
100 105 110
Ala Leu Phe Ile Tyr Val Ala Val Asp Asp Asn Gly Lys Pro Arg Pro
115 120 125
Leu Pro Pro Val Ala
130
<210> 180
<211> 133
<212> PRT
<213> Citrobacter freundii
<400> 180
Met Thr Thr Thr Asn Asn Thr Pro Gln Gly Glu Leu Val Leu Arg Thr
1 5 10 15
Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly
20 25 30
Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Gln Ala Lys Glu
35 40 45
Ile Ala His Gly Arg Val Val Thr Val Arg Val Glu Gly Met Ser Phe
50 55 60
Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys
65 70 75 80
Val Lys Arg Gly Thr Thr Ser Ile Ser Ile Asn Ile Glu Val Trp Val
85 90 95
Lys Lys Val Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu
100 105 110
Ala Leu Phe Ile Tyr Val Ala Val Asp Lys Asp Gly Lys Pro Arg Pro
115 120 125
Ile Pro Thr Leu Ala
130
<210> 181
<211> 130
<212> PRT
<213> Salmonella enterica
<400> 181
Met Asp Asn Thr Pro Gln Gly Glu Leu Val Leu Arg Thr Leu Ala Met
1 5 10 15
Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly Trp Leu Met
20 25 30
Ser Gln Met Asp Ile Gly Gly Ala Ile Leu Ala Lys Glu Ile Ala His
35 40 45
Gly Arg Val Val Thr Val Arg Val Glu Gly Met Thr Phe Leu Arg Pro
50 55 60
Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys Val Lys Arg
65 70 75 80
Gly Thr Thr Ser Ile Ser Ile Asn Ile Glu Val Trp Val Lys Lys Val
85 90 95
Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu Ala Leu Phe
100 105 110
Ile Tyr Val Ala Val Asp Pro Asp Gly Lys Pro Arg Pro Leu Pro Val
115 120 125
Gln Gly
130
<210> 182
<211> 133
<212> PRT
<213> Shigella flexneri 1235-66
<400> 182
Met Thr Thr Thr Asn Asn Thr Pro Gln Gly Glu Leu Val Leu Arg Thr
1 5 10 15
Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly
20 25 30
Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Gln Ala Lys Glu
35 40 45
Ile Ala His Gly Arg Val Val Thr Val Arg Val Glu Gly Met Ser Phe
50 55 60
Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys
65 70 75 80
Val Lys Arg Gly Thr Thr Ser Ile Ser Ile Asn Ile Glu Val Trp Val
85 90 95
Lys Lys Val Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu
100 105 110
Ala Leu Phe Ile Tyr Val Ala Val Asp Lys Asp Gly Lys Pro Arg Pro
115 120 125
Ile Pro Lys Gln Val
130
<210> 183
<211> 554
<212> PRT
<213> Ilyobacter polytropus DhaB1 protein
<400> 183
Met Lys Ser Lys Arg Phe Glu Val Leu Lys Glu Arg Pro Val Asn Lys
1 5 10 15
Asp Gly Phe Ile Ser Glu Trp Ile Glu Glu Gly Leu Ile Ala Met Glu
20 25 30
Ser Pro Asn Asp Pro Asn Pro Ser Leu Lys Ile Glu Asn Gly Gln Ile
35 40 45
Thr Glu Leu Asp Gly Lys Ser Arg Glu Glu Phe Asp Met Ile Asp Arg
50 55 60
Phe Ile Ala Asp Tyr Ala Ile Asn Met Glu Asn Ala Glu Lys Ala Met
65 70 75 80
Lys Met Ser Ser Met Glu Ile Ser Lys Lys Leu Val Asp Ile Asn Val
85 90 95
Ser Arg Asp Glu Val Leu Glu Ile Thr Thr Gly Ile Thr Pro Ala Lys
100 105 110
Ile Ile Lys Val Met Glu His Met Asn Val Val Glu Met Met Met Ala
115 120 125
Val Gln Lys Met Arg Ala Arg Lys Thr Pro Ser Asn Gln Cys His Val
130 135 140
Thr Asn Leu Arg Asp Asn Pro Val Leu Ile Ala Ala Asp Ala Ala Glu
145 150 155 160
Ala Ser Val Arg Gly Phe Asp Glu Gln Glu Thr Thr Ile Gly Ile Val
165 170 175
Arg Tyr Ala Pro Phe Asn Ala Ile Ser Ile Phe Val Gly Ser Gln Val
180 185 190
Gly Arg Gly Gly Ile Leu Thr Gln Cys Ser Val Glu Glu Ala Thr Glu
195 200 205
Leu Glu Leu Gly Met Lys Gly Phe Thr Ser Tyr Ala Glu Thr Val Ser
210 215 220
Val Tyr Gly Thr Glu Gln Val Phe Ile Asp Gly Asp Asp Thr Pro Trp
225 230 235 240
Ser Lys Ala Phe Leu Ala Ser Ala Tyr Ala Ser Arg Gly Leu Lys Met
245 250 255
Arg Phe Thr Ser Gly Thr Gly Ser Glu Ala Leu Met Gly Asn Ala Glu
260 265 270
Gly Lys Ser Met Leu Tyr Leu Glu Ala Arg Cys Ile Tyr Val Thr Arg
275 280 285
Gly Ser Gly Val Gln Gly Leu Gln Asn Gly Ser Val Ser Cys Ile Gly
290 295 300
Met Pro Gly Ser Leu Pro Gly Gly Ile Arg Ala Val Leu Ala Glu Asn
305 310 315 320
Leu Ile Ala Met Leu Leu Asp Leu Glu Cys Ala Ser Ala Asn Asp Gln
325 330 335
Thr Phe Ser His Ser Glu Tyr Arg Arg Thr Ala Arg Thr Leu Met Gln
340 345 350
Met Leu Pro Gly Thr Asp Phe Ile Phe Ser Gly Tyr Ser Ala Val Pro
355 360 365
Asn Cys Asp Asn Met Phe Ala Gly Ser Asn Phe Asp Ala Glu Asp Phe
370 375 380
Asp Asp Tyr Asn Ala Leu Gln Arg Asp Leu Lys Ile Asp Gly Gly Leu
385 390 395 400
Lys Pro Val Thr Glu Asp Glu Ile Val Lys Val Arg Asn Lys Ala Ala
405 410 415
Arg Ala Ile Gln Gly Leu Phe Lys Glu Leu Asp Leu Pro Glu Ile Thr
420 425 430
Asp Glu Glu Val Glu Ala Ala Thr Tyr Ala His Gly Ser Val Asp Met
435 440 445
Pro Ala Arg Asn Val Val Glu Asp Leu Lys Ala Ala Glu Glu Leu Leu
450 455 460
Ser Ser Gly Ile Thr Gly Val Asp Leu Val Lys Gly Leu Ser Arg Ser
465 470 475 480
Gly Phe Asp Asp Val Ala Glu His Val Leu Gly Met Leu Lys Gln Arg
485 490 495
Val Ser Gly Asp Tyr Leu Gln Thr Ser Ala Ile Leu Asp Lys Gly Phe
500 505 510
Lys Ile Lys Ser Ala Ile Asn Asp Arg Asn Asp Tyr Met Gly Pro Gly
515 520 525
Ser Gly Tyr Arg Ile Ser Glu Glu Arg Trp Glu Glu Ile Lys Asn Ile
530 535 540
Pro Ser Ala Ile Lys Pro Glu Ser Ile Glu
545 550
<210> 184
<211> 187
<212> PRT
<213> Ilyobacter polytropus DhaB2 protein
<400> 184
Met Glu Asn Lys Phe Val Pro Ser Val Lys Ile Glu Glu Ile Gly Glu
1 5 10 15
Ala Lys Lys Gly Ser Arg Ser Glu Glu Val Val Ile Gly Leu Ala Pro
20 25 30
Ala Phe Lys Lys Phe Gln His Lys Thr Ile Thr Asp Val Pro His Asp
35 40 45
Glu Val Leu Thr Glu Leu Ile Ala Gly Ile Glu Glu Glu Gly Leu Lys
50 55 60
Ala Arg Ile Val Arg Val Thr Arg Thr Ser Asp Val Ser Phe Met Ala
65 70 75 80
Leu Asp Ala Ala Lys Leu Ser Gly Ser Gly Ile Gly Ile Gly Ile Gln
85 90 95
Ser Lys Gly Thr Thr Val Ile His Gln Lys Asp Leu Leu Pro Leu Asn
100 105 110
Asn Leu Glu Leu Phe Pro Gln Ala Pro Leu Leu Thr Pro Glu Thr Phe
115 120 125
Arg Leu Ile Gly Lys Asn Ala Ala Lys Tyr Ala Lys Gly Glu Ser Pro
130 135 140
Asn Pro Val Pro Val Ala Ser Asp Gln Met Ala Arg Pro Lys Tyr Gln
145 150 155 160
Ala Lys Ala Ala Leu Leu His Ile Lys Glu Thr Lys His Val Val Gln
165 170 175
His Gly Lys Pro Val Glu Ile Lys Tyr Glu Phe
180 185
<210> 185
<211> 143
<212> PRT
<213> Ilyobacter polytropus DhaB3 protein
<400> 185
Met Asn Ile Asp Val Lys Asn Ile Asn Pro Ile Ser Asp Tyr Pro Leu
1 5 10 15
Gly Glu Lys Arg Lys Glu Trp Leu Lys Thr Ser Thr Gly Lys Thr Leu
20 25 30
Asp Glu Ile Thr Leu Glu Asn Val Ile Asn Gly Asp Ile Lys Pro Glu
35 40 45
Asp Ile Arg Ile Ser Pro Glu Thr Leu Lys Leu Gln Gly Glu Ile Ala
50 55 60
Lys Lys Gly Asn Arg Pro Thr Ile Thr Lys Asn Phe Glu Arg Ala Ser
65 70 75 80
Glu Met Val Ala Ile Pro Asp Asp Lys Ile Leu Ala Thr Tyr Asn Ala
85 90 95
Leu Arg Pro Tyr Arg Ser Ser Lys Glu Glu Leu Phe Glu Ile Ala Asp
100 105 110
Glu Leu Glu Ser Lys Tyr Ser Ala Val Val Ile Ser Ala Phe Ile Lys
115 120 125
Glu Ala Ala Glu Val Tyr Glu Gln Arg Gly Gln Leu Arg Lys Asp
130 135 140
<210> 186
<211> 1665
<212> DNA
<213> Ilyobacter polytropus dhaB1 gene
<400> 186
atgaaatcaa aaagatttga agtattgaag gaacgtcctg taaataaaga tggctttata 60
agtgaatgga tagaagaagg actaatcgca atggaaagtc ctaacgatcc taatccaagt 120
ttgaaaatag aaaatggtca aataacagag ttagacggta aaagcagaga agaatttgac 180
atgatcgaca gatttatagc agattatgca ataaatatgg aaaatgctga aaaagctatg 240
aaaatgtcat ctatggaaat atctaaaaaa ctagtagaca taaatgtatc aagagatgaa 300
gtgctggaaa taacaacagg aattacccca gcaaaaataa ttaaagttat ggaacacatg 360
aatgttgtag agatgatgat ggccgtacaa aaaatgagag ccagaaaaac tccttccaat 420
cagtgtcatg taactaactt gagagacaat cctgtattaa ttgccgctga tgctgccgaa 480
gcgtcagtaa gaggttttga tgaacaggag actacaatcg gtatagtaag atatgcacct 540
ttcaatgcca tctcaatatt tgtaggttca caagtaggta gaggaggaat actgactcag 600
tgttctgtag aagaagctac tgaattagag cttggaatga aaggattcac aagttatgca 660
gaaacagtgt ctgtatatgg tacagagcaa gtgtttatag acggtgacga cactccttgg 720
tcaaaagcct tccttgcttc agcatatgca tcaagaggat taaaaatgag atttacatct 780
ggaactggtt cagaggctct tatgggaaat gctgaaggga aatcaatgct ttaccttgaa 840
gcaagatgta tctacgtaac aagagggtct ggagtacaag gactacaaaa tggttctgta 900
agctgcatag ggatgcctgg gtcactacct ggaggaataa gggctgtact ggctgaaaac 960
ctgatagcaa tgttacttga cttagaatgt gcatcagcaa atgaccagac attctctcac 1020
tcagaatata gaaggacagc aagaactcta atgcagatgc ttcctggaac agacttcata 1080
ttctcaggat atagtgccgt accaaactgt gataacatgt ttgctggatc aaattttgat 1140
gcagaggatt ttgatgacta taatgctctt cagagagacc ttaaaataga cggtggttta 1200
aaacctgtaa ctgaagatga gattgtcaaa gtaagaaata aagcagccag agcaatacag 1260
gggttattca aagaacttga tcttcctgaa ataacagatg aagaagtgga agcagcaaca 1320
tatgcccacg gaagtgttga tatgcctgca agaaatgtgg ttgaagattt aaaagcggca 1380
gaagaacttt taagctctgg aataacagga gtagatcttg ttaaaggact tagcagaagc 1440
ggatttgacg atgtagctga gcatgtttta ggtatgttaa aacagagagt ttcaggagat 1500
tacctgcaaa cttcagctat attagacaaa ggctttaaaa taaagagtgc cataaacgat 1560
agaaatgatt acatgggtcc tggaagcgga tatagaataa gcgaggaaag atgggaagag 1620
atcaaaaata tcccatcagc tataaaacca gaaagtatag aatag 1665
<210> 187
<211> 564
<212> DNA
<213> Ilyobacter polytropus dhaB2 gene
<400> 187
atggaaaata aatttgtacc atctgtaaag atagaagaaa tcggagaagc aaaaaaagga 60
agcagatctg aagaagtagt tataggactg gctcctgcat ttaaaaaatt tcaacataaa 120
acaataacag atgtccctca cgatgaagtc ctgactgaac ttatcgcagg tatagaggaa 180
gagggattaa aggcaagaat cgtaagagta acaagaactt ctgatgtttc atttatggcg 240
ctggatgctg caaagttaag tggttctgga ataggaatag gaattcagtc aaagggaaca 300
acagtaatcc accaaaagga tctgcttcct ctaaacaatc tagaactttt cccacaggct 360
ccactattaa cacctgaaac attcagatta ataggaaaaa atgctgcaaa atatgcaaag 420
ggagaatctc caaatccagt acctgtagcc agtgaccaga tggcgagacc taaatatcag 480
gcaaaagcag cattactaca tataaaagag acaaaacatg tcgttcaaca cggaaaacca 540
gtagagataa agtatgaatt ttag 564
<210> 188
<211> 432
<212> DNA
<213> Ilyobacter polytropus dhaB3 gene
<400> 188
atgaatatag atgttaaaaa tataaatcca atctctgatt atccattagg agaaaagaga 60
aaagaatggt tgaaaacatc cacaggtaaa actttggatg aaataacttt agaaaatgta 120
ataaatggag atataaagcc tgaagatata agaatctcac ctgaaactct aaaattacag 180
ggagagatag caaagaaagg taacaggcca actataacaa agaactttga aagagccagt 240
gaaatggttg ccattccaga tgataaaata ttagcaactt acaacgcttt gagaccttac 300
agatcttcaa aggaagaatt atttgaaata gccgatgaac tagaaagtaa gtattcagct 360
gttgtaatat ctgcatttat caaggaagcc gcagaagttt atgaacaaag aggtcaactt 420
agaaaagatt ag 432
<210> 189
<211> 490
<212> PRT
<213> E.coli gabD1
<400> 189
Met Thr Ile Asn Val Ser Glu Leu Leu Ala Lys Val Pro Thr Gly Leu
1 5 10 15
Leu Ile Gly Asp Ser Trp Val Glu Ala Ser Asp Gly Gly Thr Phe Asp
20 25 30
Val Glu Asn Pro Ala Thr Gly Glu Thr Ile Ala Thr Leu Ala Ser Ala
35 40 45
Thr Ser Glu Asp Ala Leu Ala Ala Leu Asp Ala Ala Cys Ala Val Gln
50 55 60
Ala Glu Trp Ala Arg Met Pro Ala Arg Glu Arg Ser Asn Ile Leu Arg
65 70 75 80
Arg Gly Phe Glu Leu Val Ala Glu Arg Ala Glu Glu Phe Ala Thr Leu
85 90 95
Met Thr Leu Glu Met Gly Lys Pro Leu Ala Glu Ala Arg Gly Glu Val
100 105 110
Thr Tyr Gly Asn Glu Phe Leu Arg Trp Phe Ser Glu Glu Ala Val Arg
115 120 125
Leu Tyr Gly Arg Tyr Gly Thr Thr Pro Glu Gly Asn Leu Arg Met Leu
130 135 140
Thr Ala Leu Lys Pro Val Gly Pro Cys Leu Leu Ile Thr Pro Trp Asn
145 150 155 160
Phe Pro Leu Ala Met Ala Thr Arg Lys Val Ala Pro Ala Ile Ala Ala
165 170 175
Gly Cys Val Met Val Leu Lys Pro Ala Arg Leu Thr Pro Leu Thr Ser
180 185 190
Gln Tyr Phe Ala Gln Thr Met Leu Asp Ala Gly Leu Pro Ala Gly Val
195 200 205
Leu Asn Val Val Ser Gly Ala Ser Ala Ser Ala Ile Ser Asn Pro Ile
210 215 220
Met Glu Asp Asp Arg Leu Arg Lys Val Ser Phe Thr Gly Ser Thr Pro
225 230 235 240
Val Gly Gln Gln Leu Leu Lys Lys Ala Ala Asp Lys Val Leu Arg Thr
245 250 255
Ser Met Glu Leu Gly Gly Asn Ala Pro Phe Ile Val Phe Glu Asp Ala
260 265 270
Asp Leu Asp Leu Ala Ile Glu Gly Ala Met Gly Ala Lys Met Arg Asn
275 280 285
Ile Gly Glu Ala Cys Thr Ala Ala Asn Arg Phe Leu Val His Glu Ser
290 295 300
Val Ala Asp Glu Phe Gly Arg Arg Phe Ala Ala Arg Leu Glu Glu Gln
305 310 315 320
Val Leu Gly Asn Gly Leu Asp Glu Gly Val Thr Val Gly Pro Leu Val
325 330 335
Glu Glu Lys Ala Arg Asp Ser Val Ala Ser Leu Val Asp Ala Ala Val
340 345 350
Ala Glu Gly Ala Thr Val Leu Thr Gly Gly Lys Ala Gly Thr Gly Ala
355 360 365
Gly Tyr Phe Tyr Glu Pro Thr Val Leu Thr Gly Val Ser Thr Asp Ala
370 375 380
Ala Ile Leu Asn Glu Glu Ile Phe Gly Pro Val Ala Pro Ile Val Thr
385 390 395 400
Phe Gln Thr Glu Glu Glu Ala Leu Arg Leu Ala Asn Ser Thr Glu Tyr
405 410 415
Gly Leu Ala Ser Tyr Val Phe Thr Gln Asp Thr Ser Arg Ile Phe Arg
420 425 430
Val Ser Asp Gly Leu Glu Phe Gly Leu Val Gly Val Asn Ser Gly Val
435 440 445
Ile Ser Asn Ala Ala Ala Pro Phe Gly Gly Val Lys Gln Ser Gly Met
450 455 460
Gly Arg Glu Gly Gly Leu Glu Gly Ile Glu Glu Tyr Thr Ser Val Gln
465 470 475 480
Tyr Ile Gly Ile Arg Asp Pro Tyr Ala Gly
485 490
<210> 190
<211> 453
<212> PRT
<213> E.coli gabD2
<400> 190
Met Ser Leu Thr Phe Pro Val Ile Asn Pro Ser Asp Gly Ser Thr Ile
1 5 10 15
Thr Glu Leu Glu Asn His Asp Ser Thr Gln Trp Met Ser Ala Leu Ser
20 25 30
Asp Ala Val Ala Ala Gly Pro Ser Trp Ala Ala Lys Thr Pro Arg Glu
35 40 45
Arg Ser Val Val Leu Thr Ala Ile Phe Glu Ala Leu Thr Glu Arg Ala
50 55 60
Gln Glu Leu Ala Glu Ile Ile His Leu Glu Ala Gly Lys Ser Val Ala
65 70 75 80
Glu Ala Leu Gly Glu Val Ala Tyr Gly Ala Glu Tyr Phe Arg Trp Phe
85 90 95
Ala Glu Glu Ala Val Arg Leu Pro Gly Arg Tyr Gly Gln Ser Pro Ser
100 105 110
Gly Ile Gly His Ile Ala Val Thr Arg Ala Pro Val Gly Pro Val Leu
115 120 125
Ala Ile Thr Pro Trp Asn Phe Pro Ile Ala Met Ala Thr Arg Lys Ile
130 135 140
Ala Pro Ala Leu Ala Ala Gly Cys Pro Val Leu Val Lys Pro Ala Ser
145 150 155 160
Glu Thr Pro Leu Thr Met Val Lys Val Gly Glu Ile Ile Ala Ser Val
165 170 175
Phe Asp Thr Phe Asn Ile Pro Gln Gly Leu Val Ser Ile Ile Thr Thr
180 185 190
Thr Arg Asp Ala Glu Leu Ser Ala Glu Leu Met Ala Asp Pro Arg Leu
195 200 205
Ala Lys Val Thr Phe Thr Gly Ser Thr Asn Val Gly Arg Ile Leu Val
210 215 220
Arg Gln Ser Ala Asp Arg Leu Leu Arg Thr Ser Met Glu Leu Gly Gly
225 230 235 240
Asn Ala Ala Phe Val Ile Asp Glu Ala Ala Asp Leu Asp Glu Ala Val
245 250 255
Ser Gly Ala Ile Ala Ala Lys Leu Arg Asn Ala Gly Gln Val Cys Ile
260 265 270
Ala Ala Asn Arg Phe Leu Val His Glu Ser Arg Ala Ala Glu Phe Thr
275 280 285
Ser Lys Leu Ala Thr Ala Met Gln Asn Thr Pro Ile Gly Pro Val Ile
290 295 300
Ser Ala Arg Gln Arg Asp Arg Ile Ala Ala Leu Val Asp Glu Ala Ile
305 310 315 320
Thr Asp Gly Ala Arg Leu Ile Ile Gly Gly Glu Val Pro Asp Gly Ser
325 330 335
Gly Phe Phe Tyr Pro Ala Thr Ile Leu Ala Asp Val Pro Ala Gln Ser
340 345 350
Arg Ile Val His Glu Glu Ile Phe Gly Pro Val Ala Thr Ile Ala Thr
355 360 365
Phe Thr Asp Leu Ala Glu Gly Val Ala Gln Ala Asn Ser Thr Glu Phe
370 375 380
Gly Leu Ala Ala Tyr Gly Phe Ser Asn Asn Val Lys Ala Thr Gln Tyr
385 390 395 400
Met Ala Glu His Leu Glu Ala Gly Met Val Gly Ile Asn Arg Gly Ala
405 410 415
Ile Ser Asp Pro Ala Ala Pro Phe Gly Gly Ile Gly Gln Ser Gly Phe
420 425 430
Gly Arg Glu Gly Gly Thr Glu Gly Ile Glu Glu Tyr Leu Ser Val Arg
435 440 445
Tyr Leu Ala Leu Pro
450
<210> 191
<211> 521
<212> PRT
<213> E.coli gabD3
<400> 191
Met Ile Lys Arg Leu Pro Leu Gly Pro Leu Pro Lys Glu Leu His Gln
1 5 10 15
Thr Leu Leu Asp Leu Thr Ala Asn Ala Gln Asp Ala Ala Lys Val Glu
20 25 30
Val Ile Ala Pro Phe Thr Gly Glu Thr Leu Gly Phe Val Phe Asp Gly
35 40 45
Asp Glu Gln Asp Val Glu His Ala Phe Ala Leu Ser Arg Ala Ala Gln
50 55 60
Lys Lys Trp Val His Thr Thr Ala Val Glu Arg Lys Lys Ile Phe Leu
65 70 75 80
Lys Phe His Asp Leu Val Leu Lys Asn Arg Glu Leu Leu Met Asp Ile
85 90 95
Val Gln Leu Glu Thr Gly Lys Asn Arg Ala Ser Ala Ala Asp Glu Val
100 105 110
Leu Asp Val Ala Ile Thr Thr Arg Phe Tyr Ala Asn Asn Ala Gly Lys
115 120 125
Phe Leu Asn Asp Lys Lys Arg Pro Gly Ala Leu Pro Ile Ile Thr Lys
130 135 140
Asn Thr Gln Gln Tyr Val Pro Lys Gly Val Val Gly Gln Ile Thr Pro
145 150 155 160
Trp Asn Tyr Pro Leu Thr Leu Gly Val Ser Asp Ala Val Pro Ala Leu
165 170 175
Leu Ala Gly Asn Ala Val Val Ala Lys Pro Asp Leu Ala Thr Pro Phe
180 185 190
Ser Cys Leu Ile Met Val His Leu Leu Ile Glu Ala Gly Leu Pro Arg
195 200 205
Asp Leu Met Gln Val Val Thr Gly Pro Gly Asp Ile Val Gly Gly Ala
210 215 220
Ile Ala Ala Gln Cys Asp Phe Leu Met Phe Thr Gly Ser Thr Ala Thr
225 230 235 240
Gly Arg Ile Leu Gly Arg Thr Met Gly Glu Arg Leu Val Gly Phe Ser
245 250 255
Ala Glu Leu Gly Gly Lys Asn Pro Leu Ile Val Ala Lys Asp Ala Asp
260 265 270
Leu Asp Lys Val Glu Ala Glu Leu Pro Gln Ala Cys Phe Ser Asn Ser
275 280 285
Gly Gln Leu Cys Val Ser Thr Glu Arg Ile Tyr Val Glu Glu Asp Val
290 295 300
Tyr Glu Glu Val Ile Ala Arg Phe Ser Lys Ala Ala Lys Ala Met Ser
305 310 315 320
Ile Gly Ala Gly Phe Glu Trp Lys Tyr Glu Met Gly Ser Leu Ile Asn
325 330 335
Gln Ala Gln Leu Asp Arg Val Ser Thr Phe Val Asp Gln Ala Lys Ala
340 345 350
Ala Gly Ala Thr Val Leu Cys Gly Gly Lys Ser Arg Pro Asp Ile Gly
355 360 365
Pro Phe Phe Tyr Glu Pro Thr Val Leu Ala Asp Val Pro Glu Gly Thr
370 375 380
Pro Leu Leu Thr Glu Glu Val Phe Gly Pro Val Val Phe Ile Glu Lys
385 390 395 400
Val Ala Thr Leu Glu Glu Ala Val Asp Lys Ala Asn Gly Thr Pro Tyr
405 410 415
Gly Leu Asn Ala Ser Val Phe Gly Ser Ser Glu Thr Gly Asn Leu Val
420 425 430
Ala Gly Gln Leu Glu Ala Gly Gly Ile Gly Ile Asn Asp Gly Tyr Ala
435 440 445
Ala Thr Trp Ala Ser Val Ser Thr Pro Leu Gly Gly Met Lys Gln Ser
450 455 460
Gly Leu Gly His Arg His Gly Ala Glu Gly Ile Thr Lys Tyr Ala Glu
465 470 475 480
Ile Arg Asn Ile Ala Glu Gln Arg Trp Met Ser Met Arg Gly Pro Ala
485 490 495
Lys Met Pro Arg Lys Val Tyr Ser Asp Thr Val Ala Thr Ala Leu Lys
500 505 510
Leu Gly Lys Ile Phe Lys Val Leu Pro
515 520
<210> 192
<211> 1194
<212> DNA
<213> Cupriavidus necator succinate semialdehyde dehydrogenase (SSADH) protein gene: gabD gene
<400> 192
atgcacgccg ccacgcaagc catcctcacc ttcaaccatg gccgcgatcc cgagcggctg 60
acccgcaagc ttgcggcgat cgccgcggac ccgtttgcct tctttcgcgg caccaaccat 120
ctctatgccg catcgctgcg cgatgaggcg gcaatgtgca atgcgcccat cacctacgtc 180
tgcggcgatc tgcacctgga gaacttcggc agcttcaagg gcgacaacgg gctggtctat 240
ttcgacctga acgactttga cgatgccctg gtcgcgccgc ttacggtgga tgtggtccgg 300
atgctgtcga gcgtgctggt ggccgccggc cagctgggcc tttccgaggc cggcgccatg 360
cgcgcctgcg aggccatgct gtccacctat gccgccgtgc tgcagacagg caagcctcgc 420
tggctcgagc gtgccacggc ggtcggcatg gtggccaccc tgctgcgccg ggtcaagggc 480
cgcaagcgcg gcgcgctgct ggccgagctc accacgctgc gcaagggcaa acggcgcctg 540
gtatgcaacg gccgccatgc gctgccggcg gacaagccgg cccgtgagcg cgctcgcgcg 600
atccttgcgg cctactcgaa gcagggccac catggccacc gcctggccct cgacgatgcc 660
gcgcggcgcg tggcgggcat tggcagcctc gggctggaac gctatatggt gctggcgcgg 720
gacgaactga gcggcatgca gcgactggtc gacatcaagc gcgccgcgcc gagtccatgg 780
caggacctgc ccagcctgtc cctgccaccc tggggcagcg atgcaaggcg ggtggcggcg 840
gtgcagcaag tcatgcaggc ggcttcgccg gcgctgctgt cggctgtcga catgggcaag 900
gcttcctatc tggtcaagag cctgcagcca actgccgacc gcgtcgacct ggcgcattgc 960
agcaactttg cagcgctacg cgagttgctg ggcaccatgg cgcatgccgc ggcatgggcg 1020
catctgcgtg gctgcgggca ccaggccgcg gaccggatcg agcagctgca ggcgtttgcc 1080
ggcggcaccc gctggcgcac cggcgtcctg cggctggcac ggcatggctg cgcggtgtcg 1140
gtggtgcagt ggaaggcgta tgcggacgat taccgcgagg cgcggggagg gtga 1194
<210> 193
<211> 397
<212> PRT
<213> Cupriavidus necator gabD protein
<400> 193
Met His Ala Ala Thr Gln Ala Ile Leu Thr Phe Asn His Gly Arg Asp
1 5 10 15
Pro Glu Arg Leu Thr Arg Lys Leu Ala Ala Ile Ala Ala Asp Pro Phe
20 25 30
Ala Phe Phe Arg Gly Thr Asn His Leu Tyr Ala Ala Ser Leu Arg Asp
35 40 45
Glu Ala Ala Met Cys Asn Ala Pro Ile Thr Tyr Val Cys Gly Asp Leu
50 55 60
His Leu Glu Asn Phe Gly Ser Phe Lys Gly Asp Asn Gly Leu Val Tyr
65 70 75 80
Phe Asp Leu Asn Asp Phe Asp Asp Ala Leu Val Ala Pro Leu Thr Val
85 90 95
Asp Val Val Arg Met Leu Ser Ser Val Leu Val Ala Ala Gly Gln Leu
100 105 110
Gly Leu Ser Glu Ala Gly Ala Met Arg Ala Cys Glu Ala Met Leu Ser
115 120 125
Thr Tyr Ala Ala Val Leu Gln Thr Gly Lys Pro Arg Trp Leu Glu Arg
130 135 140
Ala Thr Ala Val Gly Met Val Ala Thr Leu Leu Arg Arg Val Lys Gly
145 150 155 160
Arg Lys Arg Gly Ala Leu Leu Ala Glu Leu Thr Thr Leu Arg Lys Gly
165 170 175
Lys Arg Arg Leu Val Cys Asn Gly Arg His Ala Leu Pro Ala Asp Lys
180 185 190
Pro Ala Arg Glu Arg Ala Arg Ala Ile Leu Ala Ala Tyr Ser Lys Gln
195 200 205
Gly His His Gly His Arg Leu Ala Leu Asp Asp Ala Ala Arg Arg Val
210 215 220
Ala Gly Ile Gly Ser Leu Gly Leu Glu Arg Tyr Met Val Leu Ala Arg
225 230 235 240
Asp Glu Leu Ser Gly Met Gln Arg Leu Val Asp Ile Lys Arg Ala Ala
245 250 255
Pro Ser Pro Trp Gln Asp Leu Pro Ser Leu Ser Leu Pro Pro Trp Gly
260 265 270
Ser Asp Ala Arg Arg Val Ala Ala Val Gln Gln Val Met Gln Ala Ala
275 280 285
Ser Pro Ala Leu Leu Ser Ala Val Asp Met Gly Lys Ala Ser Tyr Leu
290 295 300
Val Lys Ser Leu Gln Pro Thr Ala Asp Arg Val Asp Leu Ala His Cys
305 310 315 320
Ser Asn Phe Ala Ala Leu Arg Glu Leu Leu Gly Thr Met Ala His Ala
325 330 335
Ala Ala Trp Ala His Leu Arg Gly Cys Gly His Gln Ala Ala Asp Arg
340 345 350
Ile Glu Gln Leu Gln Ala Phe Ala Gly Gly Thr Arg Trp Arg Thr Gly
355 360 365
Val Leu Arg Leu Ala Arg His Gly Cys Ala Val Ser Val Val Gln Trp
370 375 380
Lys Ala Tyr Ala Asp Asp Tyr Arg Glu Ala Arg Gly Gly
385 390 395
<210> 194
<211> 1473
<212> DNA
<213> E.coli gabD1
<400> 194
atgactatta atgtctccga actacttgcc aaagtcccca cgggtctact gattggtgat 60
tcctgggtgg aagcatccga cggcggtact ttcgatgtgg aaaacccagc gacgggtgaa 120
acaatcgcaa cgctcgcgtc tgctacttcc gaggatgcac tggctgctct tgatgctgca 180
tgcgctgttc aggccgagtg ggctaggatg ccagcgcgcg agcgttctaa tattttacgc 240
cgcggttttg agctcgtagc agaacgtgca gaagagttcg ccaccctcat gaccttggaa 300
atgggcaagc ctttggctga agctcgcggc gaagtcacct acggcaacga attcctgcgc 360
tggttctctg aggaagcagt tcgtctgtat ggccgttacg gaaccacacc agaaggcaac 420
ttgcggatgc tgaccgccct caagccagtt ggcccgtgcc tcctgatcac cccatggaac 480
ttcccactag caatggctac ccgcaaggtc gcacctgcga tcgctgcagg ttgtgtcatg 540
gtgctcaagc cagctcgact taccccgctg acctcccagt attttgctca gaccatgctt 600
gatgccggtc ttccagcagg tgtcctcaat gtggtctccg gtgcttccgc ctctgcgatt 660
tccaacccga ttatggaaga cgatcgcctt cgtaaagtct ccttcaccgg ctccacccca 720
gttggccagc agctgctcaa aaaggctgcc gataaagttc tgcgcacctc catggaactt 780
ggtggcaacg cacctttcat tgtcttcgag gacgccgacc tagatctcgc gatcgaaggt 840
gccatgggtg ccaaaatgcg caacatcggc gaagcttgca ccgcagccaa ccgtttctta 900
gtccacgaat ccgtcgccga tgaattcggc cgtcgcttcg ctgcccgcct tgaagagcaa 960
gtcctaggca acggcctcga cgaaggcgtc accgtgggcc ccctggttga ggaaaaagca 1020
cgagacagcg ttgcatcgct tgtcgacgcc gccgtcgccg aaggtgccac cgtcctcacc 1080
ggcggcaagg ccggcacagg tgcaggctac ttctacgaac caacggtgct cacgggagtt 1140
tcaacagatg cggctatcct gaacgaagag atcttcggtc ccgtcgcacc gatcgtcacc 1200
ttccaaaccg aggaagaagc cctgcgtcta gccaactcca ccgaatacgg actggcctcc 1260
tatgtgttca cccaggacac ctcacgtatt ttccgcgtct ccgatggtct cgagttcggc 1320
ctagtgggcg tcaattccgg tgtcatctct aacgctgctg caccttttgg tggcgtaaaa 1380
caatccggaa tgggccgcga aggtggtctc gaaggaatcg aggagtacac ctccgtgcag 1440
tacatcggta tccgggatcc ttacgccggc tag 1473
<210> 195
<211> 1362
<212> DNA
<213> E.coli gabD2 gene
<400> 195
gtgtctttga ccttcccagt aatcaacccc agcgatggct ccaccatcac cgagctagaa 60
aaccacgatt ccacccagtg gatgtccgcg ctctctgatg cagttgcagc tggtccttca 120
tgggctgcga aaactccccg cgaaagatcc gtggtactca ccgcaatctt cgaagcactg 180
accgaacgcg cccaagaact tgcagagatc atccacctgg aagctggaaa atccgttgca 240
gaagctcttg gtgaagtcgc ttatggtgca gaatacttcc gttggtttgc ggaagaagca 300
gtgcgcctgc ccggccgcta cggacagtca ccttccggaa tcggtcacat cgccgtcacc 360
cgcgcacccg tgggaccagt gctggcgatc accccatgga atttccccat cgccatggcc 420
acccgcaaaa tcgccccagc cctggccgct ggttgccccg tgttggtgaa acctgcttcc 480
gaaaccccac tgaccatggt caaagtgggg gagatcatcg cctccgtctt tgataccttt 540
aatatcccgc agggcttggt ctcaatcatc accaccactc gagatgcaga gctatcggca 600
gaactcatgg ctgatcctcg cttggctaaa gtcaccttca ctggatcaac caacgtggga 660
cgcatcctgg tccgccaatc cgcggaccga ctgctgcgca cctccatgga actcggcgga 720
aatgcagctt ttgttatcga cgaagccgca gacctcgacg aagccgtatc cggtgccatc 780
gccgcaaaac tccgcaacgc cggccaagta tgcatcgcag ctaaccgttt cttggttcat 840
gaatcccgcg ctgccgaatt cacctcaaag ctggcgacag ccatgcagaa cactcccatt 900
gggccggtga tttctgcccg ccaacgcgac cggatcgcag cactagtgga tgaagccatc 960
accgacggcg cccgcctcat catcggtggg gaggtccccg acggctccgg cttcttctat 1020
ccagccacca tcttggccga tgtccctgca cagtcacgga ttgtgcatga ggaaatcttc 1080
ggacctgtgg ccaccattgc cactttcacc gacttggccg aaggcgttgc acaagcaaat 1140
tccaccgaat tcggcctcgc agcctacgga ttcagcaaca atgtgaaagc aacacagtac 1200
atggcggaac acttggaagc cggaatggtc ggaatcaaca gaggcgccat ctctgaccca 1260
gcagcacctt ttggcggcat cggacaatcc ggcttcggca gagaaggcgg aaccgaagga 1320
atcgaagaat atctctccgt gcgttacctc gctttgccgt ga 1362
<210> 196
<211> 1566
<212> DNA
<213> E.coli gabD3 gene
<400> 196
atgatcaaac gtcttccttt aggtccgctg cctaaagaac ttcatcagac tctgcttgat 60
ctgaccgcaa atgcccaaga tgcggcgaaa gtggaggtta tagcgccatt tactggcgag 120
accctcggat ttgtttttga tggtgatgag caagacgtcg agcatgcttt tgcactttca 180
agggcagccc agaaaaagtg ggtgcacacc acggcagtgg aacggaagaa gatcttcctg 240
aagtttcatg atctggtatt gaaaaaccgt gagctgctca tggacatcgt gcagttggaa 300
acaggcaaaa atcgagcatc ggctgccgat gaggtgttgg acgttgcgat caccacccgc 360
ttctacgcaa acaatgcagg aaagttttta aatgacaaga aacgccccgg cgcgcttccg 420
atcatcacga aaaacacaca acagtatgtg cccaagggag tggtcgggca gatcacgccg 480
tggaattacc ctttaacttt gggagtatct gatgctgttc cggcgctgct ggcaggaaac 540
gcagtggtgg ctaaacctga cctcgcgaca cctttctcct gcttgatcat ggtgcacctg 600
ctcattgaag ccggtctgcc gcgtgatttg atgcaggttg tcaccggccc tggcgatatt 660
gttggcggtg cgattgcagc tcagtgtgat ttcctcatgt tcactggatc cacggccacg 720
ggccggatct tgggtcggac aatgggtgag cgtttggtgg gtttctctgc ggaattaggc 780
ggaaagaacc ctcttattgt ggccaaggat gcagatctgg acaaggtgga agctgagctt 840
ccgcaggcgt gtttttccaa ctcggggcaa ttgtgtgtct ccactgaacg tatttatgtc 900
gaggaagacg tgtacgagga ggtgattgca cggtttagca aggcggcgaa agccatgtcc 960
attggtgccg gatttgagtg gaaatatgag atgggttcgt tgatcaatca ggcgcagctg 1020
gatcgggtga gcacctttgt tgatcaggct aaagctgcgg gcgccacggt gctgtgcggt 1080
ggcaagtcac gccctgatat tggtcccttc ttctatgagc ccacggtatt ggcggatgtc 1140
ccagagggca ccccactgct cacggaggaa gtcttcgggc cggtggtgtt catcgaaaag 1200
gtagccacac tggaagaagc cgtcgataag gcaaatggca cgccctacgg cctgaatgcg 1260
tccgtctttg ggtcgtcgga aaccggcaat cttgttgcag gccagctgga agctggcggt 1320
atcggtatta atgatggcta cgccgcgacg tgggcgagcg tgtccacgcc tctgggtggc 1380
atgaagcagt cggggctggg gcaccgccat ggtgcggagg gaattacaaa atatgcggag 1440
atccgaaaca tcgcggagca gcgctggatg tctatgcgtg ggccggccaa aatgccgcga 1500
aaggtgtact cagacaccgt ggccacagcg ctaaagctgg gcaaaatctt taaagttttg 1560
ccgtag 1566
<210> 197
<211> 607
<212> PRT
<213> K.pneumoniae gdrA protein
<400> 197
Met Pro Leu Ile Ala Gly Ile Asp Ile Gly Asn Ala Thr Thr Glu Val
1 5 10 15
Ala Leu Ala Ser Asp Asp Pro Gln Ala Arg Ala Phe Val Ala Ser Gly
20 25 30
Ile Val Ala Thr Thr Gly Met Lys Gly Thr Arg Asp Asn Ile Ala Gly
35 40 45
Thr Leu Ala Ala Leu Glu Gln Ala Leu Ala Lys Thr Pro Trp Ser Val
50 55 60
Ser Asp Val Ser Arg Ile Tyr Leu Asn Glu Ala Ala Pro Val Ile Gly
65 70 75 80
Asp Val Ala Met Glu Thr Ile Thr Glu Thr Ile Ile Thr Glu Ser Thr
85 90 95
Met Ile Gly His Asn Pro Gln Thr Pro Gly Gly Val Gly Val Gly Val
100 105 110
Gly Thr Thr Ile Ala Leu Gly Arg Leu Ala Thr Leu Pro Ala Ala Gln
115 120 125
Tyr Ala Glu Gly Trp Ile Val Leu Ile Asp Asp Ala Val Asp Phe Leu
130 135 140
Asp Ala Val Trp Trp Leu Asn Glu Ala Leu Asp Arg Gly Ile Asn Val
145 150 155 160
Val Ala Ala Ile Leu Lys Lys Asp Asp Gly Val Leu Val Asn Asn Arg
165 170 175
Leu Arg Lys Thr Leu Pro Val Val Asp Glu Val Thr Leu Leu Glu Gln
180 185 190
Val Pro Glu Gly Val Met Ala Ala Val Glu Val Ala Ala Pro Gly Gln
195 200 205
Val Val Arg Ile Leu Ser Asn Pro Tyr Gly Ile Ala Thr Phe Phe Gly
210 215 220
Leu Ser Pro Glu Glu Thr Gln Ala Ile Val Pro Ile Ala Arg Ala Leu
225 230 235 240
Ile Gly Asn Arg Ser Ala Val Val Leu Lys Thr Pro Gln Gly Asp Val
245 250 255
Gln Ser Arg Val Ile Pro Ala Gly Asn Leu Tyr Ile Ser Gly Glu Lys
260 265 270
Arg Arg Gly Glu Ala Asp Val Ala Glu Gly Ala Glu Ala Ile Met Gln
275 280 285
Ala Met Ser Ala Cys Ala Pro Val Arg Asp Ile Arg Gly Glu Pro Gly
290 295 300
Thr His Ala Gly Gly Met Leu Glu Arg Val Arg Lys Val Met Ala Ser
305 310 315 320
Leu Thr Asp His Glu Met Ser Ala Ile Tyr Ile Gln Asp Leu Leu Ala
325 330 335
Val Asp Thr Phe Ile Pro Arg Lys Val Gln Gly Gly Met Ala Gly Glu
340 345 350
Cys Ala Met Glu Asn Ala Val Gly Met Ala Ala Met Val Lys Ala Asp
355 360 365
Arg Leu Gln Met Gln Val Ile Ala Arg Glu Leu Ser Ala Arg Leu Gln
370 375 380
Thr Glu Val Val Val Gly Gly Val Glu Ala Asn Met Ala Ile Ala Gly
385 390 395 400
Ala Leu Thr Thr Pro Gly Cys Ala Ala Pro Leu Ala Ile Leu Asp Leu
405 410 415
Gly Ala Gly Ser Thr Asp Ala Ala Ile Val Asn Ala Glu Gly Gln Ile
420 425 430
Thr Ala Val His Leu Ala Gly Ala Gly Asn Met Val Ser Leu Leu Ile
435 440 445
Lys Thr Glu Leu Gly Leu Glu Asp Leu Ser Leu Ala Glu Ala Ile Lys
450 455 460
Lys Tyr Pro Leu Ala Lys Val Glu Ser Leu Phe Ser Ile Arg His Glu
465 470 475 480
Asn Gly Ala Val Glu Phe Phe Arg Glu Ala Leu Ser Pro Ala Val Phe
485 490 495
Ala Lys Val Val Tyr Ile Lys Glu Gly Glu Leu Val Pro Ile Asp Asn
500 505 510
Ala Ser Pro Leu Glu Lys Ile Arg Leu Val Arg Arg Gln Ala Lys Glu
515 520 525
Lys Val Phe Val Thr Asn Cys Leu Arg Ala Leu Arg Gln Val Ser Pro
530 535 540
Gly Gly Ser Ile Arg Asp Ile Ala Phe Val Val Leu Val Gly Gly Ser
545 550 555 560
Ser Leu Asp Phe Glu Ile Pro Gln Leu Ile Thr Glu Ala Leu Ser His
565 570 575
Tyr Gly Val Val Ala Gly Gln Gly Asn Ile Arg Gly Thr Glu Gly Pro
580 585 590
Arg Asn Ala Val Ala Thr Gly Leu Leu Leu Ala Gly Gln Ala Asn
595 600 605
<210> 198
<211> 117
<212> PRT
<213> K.pneumoniae gdrB protein
<400> 198
Met Ser Leu Ser Pro Pro Gly Val Arg Leu Phe Tyr Asp Pro Arg Gly
1 5 10 15
His His Ala Gly Ala Ile Asn Glu Leu Cys Trp Gly Leu Glu Glu Gln
20 25 30
Gly Val Pro Cys Gln Thr Ile Thr Tyr Asp Gly Gly Gly Asp Ala Ala
35 40 45
Ala Leu Gly Ala Leu Ala Ala Arg Ser Ser Pro Leu Arg Val Gly Ile
50 55 60
Gly Leu Ser Ala Ser Gly Glu Ile Ala Leu Thr His Ala Gln Leu Pro
65 70 75 80
Ala Asp Ala Pro Leu Ala Thr Gly His Val Thr Asp Ser Asp Asp His
85 90 95
Leu Arg Thr Leu Gly Ala Asn Ala Gly Gln Leu Val Lys Val Leu Pro
100 105 110
Leu Ser Glu Arg Asn
115
<210> 199
<211> 607
<212> PRT
<213> Ilyobacter polytropus gdrA protien
<400> 199
Met Lys Ile Ile Val Gly Val Asp Ile Gly Asn Ala Thr Thr Glu Val
1 5 10 15
Ala Leu Ala Lys Val Asp Asn Ile Glu Cys Lys Phe Leu Ser Ser Ala
20 25 30
Leu His Glu Thr Thr Gly Leu Lys Gly Thr Lys Asp Asn Val Leu Gly
35 40 45
Ile Lys Arg Ala Ile Lys Lys Ala Met Lys Arg Ala Asp Leu Lys Asn
50 55 60
Ala Asp Leu Ser Leu Ile Arg Ile Asn Glu Ala Thr Pro Val Ile Gly
65 70 75 80
Asp Val Ser Met Glu Thr Ile Thr Glu Thr Ile Ile Thr Glu Ser Thr
85 90 95
Met Ile Gly His Asn Pro Ser Thr Pro Gly Gly Ile Gly Leu Gly Ile
100 105 110
Gly Glu Thr Ile Leu Phe Gln Glu Leu Gly Asn Phe Glu Asn Asp Lys
115 120 125
Asp Tyr Ile Val Ile Val Glu Lys Ser Phe Ser Phe Leu Glu Val Ala
130 135 140
His Arg Ile Asn Glu Ala Phe Lys Asn Gly Cys Lys Ile Lys Gly Ala
145 150 155 160
Ile Ile Gln Lys Asp Asp Gly Val Leu Ile Asn Asn Arg Leu Ile Asn
165 170 175
Lys Ile Pro Ile Val Asp Glu Val Leu Phe Val Lys Lys Val Pro Thr
180 185 190
Gly Met Lys Ala Ala Val Glu Val Ala Pro Gln Gly Lys Ile Ile Glu
195 200 205
Val Ile Ser Asn Pro Tyr Gly Ile Ala Thr Ile Phe Ser Leu Thr Ser
210 215 220
Glu Glu Thr Lys Lys Ile Val Pro Ile Ser Lys Ala Leu Ile Gly Asn
225 230 235 240
Arg Ser Gly Val Val Ile Lys Thr Pro His Gly Asp Val Lys Glu Lys
245 250 255
Val Ile Pro Ala Gly Arg Ile Gln Ile Asp Gly Asn Tyr Arg Ser Lys
260 265 270
Ser Val Asn Ile Glu Glu Gly Ser Lys Arg Ile Met Lys Ala Leu Gly
275 280 285
Ser Ile Glu His Val Gln Asp Ile Asn Gly Glu Ser Gly Thr Asn Ile
290 295 300
Gly Gly Met Leu Lys Asn Val Lys Ser Val Met Gly Asn Phe Thr Asn
305 310 315 320
Glu Ser Ile Asp Asn Ile Lys Ile Lys Asp Ile Leu Ala Val Asp Thr
325 330 335
Phe Val Pro Gln Lys Ile Lys Gly Gly Ile Ala Glu Glu Phe Val Phe
340 345 350
Glu Asn Ala Val Gly Ile Ala Ala Met Val Asn Thr Lys Lys Asn Gln
355 360 365
Met Ser Glu Val Ala Lys Glu Ile Glu Lys Glu Leu Gly Val Lys Val
370 375 380
Glu Val Gly Gly Val Glu Ala Asp Met Ala Ile Thr Gly Ala Leu Thr
385 390 395 400
Thr Pro Gly Thr Gly Thr Pro Leu Val Ile Val Asp Ile Gly Ala Gly
405 410 415
Ser Thr Asp Ala Cys Ser Ile Asp Arg Tyr Gly Asn Lys Glu Leu Val
420 425 430
His Leu Ala Gly Ala Gly Asn Met Thr Thr Leu Leu Ile Gln Lys Glu
435 440 445
Leu Gly Ile Glu Asp Phe Asn Leu Ala Glu Asp Ile Lys Lys Tyr Pro
450 455 460
Leu Ala Lys Val Glu Ser Leu Phe Tyr Ile Arg His Glu Asp Gly Asn
465 470 475 480
Val Gln Phe Phe Glu Asn Ser Leu Ser Pro Lys Val Phe Ala Lys Asn
485 490 495
Val Leu Ile Lys Glu Gly Glu Leu Ile Pro Ile Asp Leu Asp Met Ser
500 505 510
Leu Glu Lys Ile Arg Ile Ile Arg Arg Ser Ala Lys Arg Lys Ile Phe
515 520 525
Ile Thr Asn Val Leu Arg Ser Leu Arg Lys Val Ser His Thr Lys Asn
530 535 540
Ile Arg Asp Phe Glu Phe Val Val Ile Val Gly Gly Ser Ala Leu Asp
545 550 555 560
Phe Glu Ile Ser Gln Met Ile Thr Glu Ala Leu Ser Glu Tyr Gly Ile
565 570 575
Val Ala Gly Cys Gly Asn Ile Arg Gly Thr Glu Gly Pro Arg Asn Ala
580 585 590
Val Ala Thr Gly Leu Val Met Gly Val Asn Asp Gly Gln Gln Ala
595 600 605
<210> 200
<211> 107
<212> PRT
<213> Ilyobacter polytropus gdrB
<400> 200
Met Asp Asn Arg Pro Asn Ile Thr Leu Phe Cys Ser Asp Asn Ile Asp
1 5 10 15
Arg Glu Tyr Ile Asn Glu Ile Leu Trp Gly Ile Glu Glu Glu Glu Ile
20 25 30
Pro Tyr Leu Leu Lys Ile Val Pro Ser Lys Glu Val Val Lys Glu Asn
35 40 45
Tyr Val Ser Gly Thr Leu Glu Ile Gly Ile Gly Val Leu Glu Asn Gly
50 55 60
Asp Ala Leu Leu Thr Thr Arg Lys Tyr Asp Lys Glu Tyr Ile Gln Lys
65 70 75 80
Ala Asn Ile Phe Val Glu Lys Asn Lys Leu Arg Asp Leu Gly Ser Asn
85 90 95
Gly Ala Arg Leu Val Lys Gly Leu Pro Leu Arg
100 105
<210> 201
<211> 1824
<212> DNA
<213> Ilyobacter polytropus gdrA gene
<400> 201
atgaagatca tagtgggtgt agatattgga aatgctacaa cagaagtagc tttggcaaag 60
gtagacaata tagaatgtaa gtttttatcc agtgccttac atgaaacaac aggtttaaaa 120
ggtactaaag ataatgtttt gggaataaaa agagccatta agaaggcaat gaaaagagct 180
gatttaaaaa atgcagattt atctttaatc aggataaatg aagctactcc tgttatagga 240
gacgtttcta tggaaactat aacagaaaca ataattacag agtctactat gattggacat 300
aacccttcaa ctcctggggg aataggtctt gggataggag aaacaatcct attccaagag 360
cttggaaatt ttgaaaatga taaagattac atagtaatag tggaaaaaag tttcagcttc 420
ttagaggtag ctcacagaat caatgaagct tttaaaaatg gatgcaaaat aaagggtgct 480
attattcaaa aagatgatgg ggttctcata aataacagac tcataaataa aatccccata 540
gttgatgagg tactttttgt taaaaaagta cctacaggga tgaaggctgc tgtagaagta 600
gctccacagg gaaaaataat agaggttatt tcaaatccat atggcattgc cacaattttt 660
tccctcactt cagaagagac taaaaaaata gttcctattt ctaaagcact tataggcaac 720
aggtctggag tagttatcaa gacacctcac ggagatgtaa aagagaaggt tatccctgct 780
ggaaggatac agattgacgg aaactacagg tcaaaaagtg taaatataga agagggttcc 840
aaaagaataa tgaaagccct gggaagtatt gagcatgtcc aagatataaa tggagaatct 900
ggaaccaata tcggaggaat gctaaaaaat gtaaaaagtg taatggggaa tttcaccaat 960
gagtccattg ataatataaa aataaaagac atattggcag tagatacctt tgtcccacaa 1020
aagataaagg ggggaattgc agaagaattt gtatttgaaa atgctgtagg aatagctgca 1080
atggtaaata ccaaaaaaaa tcaaatgtcc gaagtagcga aagagattga aaaagaactg 1140
ggagtaaaag tagaagtagg aggagtagag gcagatatgg ctataaccgg tgctctaact 1200
actccaggca caggaacacc tctggtaatt gtagatatag gagcaggttc gacagatgca 1260
tgttccattg acagatatgg aaataaagaa ctggttcatc tggccggagc tggtaatatg 1320
acaacacttc ttattcaaaa agagctgggt atagaggatt ttaatcttgc tgaagatata 1380
aaaaaatatc ctctggcaaa agtagaatct ctattttata taagacacga ggatggaaat 1440
gttcaatttt ttgaaaactc tctttctccg aaagtatttg ctaaaaatgt ccttataaaa 1500
gaaggtgaac ttattccaat cgaccttgat atgtctctgg aaaaaatcag aattatcaga 1560
aggtctgcca aaagaaaaat ttttataacc aatgtactta gatcattaag gaaagtttct 1620
catacaaaaa atattaggga ttttgaattt gtagttattg ttggaggatc tgcattggat 1680
tttgaaatat ctcagatgat aactgaagct ttatctgagt atggaatagt agcaggatgc 1740
ggaaatataa gaggaacaga gggccctaga aatgctgtag ccactggact tgtaatgggg 1800
gtgaatgatg gacaacaggc ctaa 1824
<210> 202
<211> 324
<212> DNA
<213> Ilyobacter polytropus gdrB gene
<400> 202
atggacaaca ggcctaatat aacattattt tgctcagata atattgacag ggaatatatt 60
aatgaaattt tgtggggtat agaggaggaa gagataccat atcttctgaa aattgtacct 120
tctaaagaag ttgtcaaaga aaattatgtt tcaggaactc tagagatagg tatcggagta 180
ttagaaaatg gcgacgccct tctaacaaca aggaagtacg ataaggaata tatacaaaag 240
gcaaacattt ttgtagaaaa aaataaattg agagatttag gaagcaacgg agcaagactt 300
gtaaagggtc tgccacttag ataa 324
<210> 203
<211> 1824
<212> DNA
<213> K.pneumoniae gdrA gene
<400> 203
atgccgttaa tagccgggat tgatatcggc aacgccacca ccgaggtggc gctggcgtcc 60
gacgacccgc aggcgagggc gtttgttgcc agcgggatcg tcgcgacgac gggcatgaaa 120
gggacgcggg acaatatcgc cgggaccctc gccgcgctgg agcaggccct ggcgaaaaca 180
ccgtggtcgg tgagcgatgt ctctcgcatc tatcttaacg aagccgcgcc ggtgattggc 240
gatgtggcga tggagaccat caccgagacc attatcaccg aatcgaccat gatcggtcat 300
aacccgcaga cgccgggcgg ggtgggcgtt ggcgtgggga cgactatcgc cctcgggcgg 360
ctggcgacgc tgccggcggc gcagtatgcc gaggggtgga tcgtactgat tgacgacgcc 420
gtcgatttcc ttgacgccgt gtggtggctc aatgaggcgc tcgaccgggg gatcaacgtg 480
gtggcggcga tcctcaaaaa ggacgacggc gtgctggtga acaaccgcct gcgtaaaacc 540
ctgccggtgg tagatgaagt gacgctgctg gagcaggtcc ccgagggggt aatggcggcg 600
gtggaagtgg ccgcgccggg ccaggtggtg cggatcctgt cgaatcccta cgggatcgcc 660
accttcttcg ggctaagccc ggaagagacc caggccatcg tccccatcgc ccgcgccctg 720
attggcaacc gttcagcggt ggtgctcaag accccgcagg gggatgtgca gtcgcgggtg 780
atcccggcgg gcaacctcta cattagcggc gaaaagcgcc gcggagaggc cgatgtcgcc 840
gagggcgcgg aagccatcat gcaggcgatg agcgcctgcg ctccggtacg cgacatccgc 900
ggcgaaccgg gcactcacgc cggcggcatg cttgagcggg tgcgcaaggt aatggcgtcc 960
ctgaccgacc atgagatgag cgcgatatac atccaggatc tgctggcggt ggatacgttt 1020
attccgcgca aggtgcaggg cgggatggcc ggcgagtgcg ccatggaaaa tgccgtcggg 1080
atggcggcga tggtgaaagc ggatcgtctg caaatgcagg ttatcgcccg cgaactgagc 1140
gcccgactgc agaccgaggt ggtggtgggc ggcgtggagg ccaacatggc catcgccggg 1200
gcgttaacca ctcccggctg tgcggcgccg ctggcgatcc tcgacctcgg cgccggctcg 1260
acggatgcgg cgatcgtcaa cgcggagggg cagataacgg cggtccatct cgccggggcg 1320
gggaatatgg tcagcctgtt gattaaaacc gagctgggcc tcgaggatct ttcgctggcg 1380
gaagcgataa aaaaataccc gctggccaaa gtggaaagcc tgttcagtat tcgtcacgag 1440
aatggcgcgg tggagttctt tcgggaagcc ctcagcccgg cggtgttcgc caaagtggtg 1500
tacatcaagg agggcgaact ggtgccgatc gataacgcca gcccgctgga aaaaattcgt 1560
ctcgtgcgcc ggcaggcgaa agagaaagtg tttgtcacca actgcctgcg cgcgctgcgc 1620
caggtctcac ccggcggttc cattcgcgat atcgcctttg tggtgctggt gggcggctca 1680
tcgctggact ttgagatccc gcagcttatc acggaagcct tgtcgcacta tggcgtggtc 1740
gccgggcagg gcaatattcg gggaacagaa gggccgcgca acgcggtcgc caccgggctg 1800
ctactggccg gtcaggcgaa ttaa 1824
<210> 204
<211> 354
<212> DNA
<213> K.pneumoniae gdrB gene
<400> 204
atgtcgcttt caccgccagg cgtacgcctg ttttacgatc cgcgcgggca ccatgccggc 60
gccatcaatg agctgtgctg ggggctggag gagcaggggg tcccctgcca gaccataacc 120
tatgacggag gcggtgacgc cgctgcgctg ggcgccctgg cggccagaag ctcgcccctg 180
cgggtgggta ttgggctcag cgcgtccggc gagatagccc tcactcatgc ccagctgccg 240
gcggacgcgc cgctggctac cggacacgtc accgatagcg acgatcatct gcgtacgctc 300
ggcgccaacg ccgggcagct ggttaaagtc ctgccgttaa gtgagagaaa ctga 354
<210> 205
<211> 314
<212> PRT
<213> Corynebacterium glutamicum
<400> 205
Met Lys Glu Thr Val Gly Asn Lys Ile Val Leu Ile Gly Ala Gly Asp
1 5 10 15
Val Gly Val Ala Tyr Ala Tyr Ala Leu Ile Asn Gln Gly Met Ala Asp
20 25 30
His Leu Ala Ile Ile Asp Ile Asp Glu Lys Lys Leu Glu Gly Asn Val
35 40 45
Met Asp Leu Asn His Gly Val Val Trp Ala Asp Ser Arg Thr Arg Val
50 55 60
Thr Lys Gly Thr Tyr Ala Asp Cys Glu Asp Ala Ala Met Val Val Ile
65 70 75 80
Cys Ala Gly Ala Ala Gln Lys Pro Gly Glu Thr Arg Leu Gln Leu Val
85 90 95
Asp Lys Asn Val Lys Ile Met Lys Ser Ile Val Gly Asp Val Met Asp
100 105 110
Ser Gly Phe Asp Gly Ile Phe Leu Val Ala Ser Asn Pro Val Asp Ile
115 120 125
Leu Thr Tyr Ala Val Trp Lys Phe Ser Gly Leu Glu Trp Asn Arg Val
130 135 140
Ile Gly Ser Gly Thr Val Leu Asp Ser Ala Arg Phe Arg Tyr Met Leu
145 150 155 160
Gly Glu Leu Tyr Glu Val Ala Pro Ser Ser Val His Ala Tyr Ile Ile
165 170 175
Gly Glu His Gly Asp Thr Glu Leu Pro Val Leu Ser Ser Ala Thr Ile
180 185 190
Ala Gly Val Ser Leu Ser Arg Met Leu Asp Lys Asp Pro Glu Leu Glu
195 200 205
Gly Arg Leu Glu Lys Ile Phe Glu Asp Thr Arg Asp Ala Ala Tyr His
210 215 220
Ile Ile Asp Ala Lys Gly Ser Thr Ser Tyr Gly Ile Gly Met Gly Leu
225 230 235 240
Ala Arg Ile Thr Arg Ala Ile Leu Gln Asn Gln Asp Val Ala Val Pro
245 250 255
Val Ser Ala Leu Leu His Gly Glu Tyr Gly Glu Glu Asp Ile Tyr Ile
260 265 270
Gly Thr Pro Ala Val Val Asn Arg Arg Gly Ile Arg Arg Val Val Glu
275 280 285
Leu Glu Ile Thr Asp His Glu Met Glu Arg Phe Lys His Ser Ala Asn
290 295 300
Thr Leu Arg Glu Ile Gln Lys Gln Phe Phe
305 310
<210> 206
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> Forward primer
<400> 206
tcatcaccac agccaggatc cgatgagaaa ggttcccatt att 43
<210> 207
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> reverse primer
<400> 207
gcattatgcg gccgcaagct tttaggactt catttccttc ag 42
<210> 208
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 208
tcatcaccac agccaggatc cgatgaaacc tgtaaaacca cctc 44
<210> 209
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 209
gcattatgcg gccgcaagct tttaatgagc cgcttcaggc a 41
<210> 210
<211> 48
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 210
tcatcaccac agccaggatc cgatgaaggt gatcaccgca cgcgaagc 48
<210> 211
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 211
gcattatgcg gccgcaagct tttacaggtg caggggcccg gc 42
<210> 212
<211> 52
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 212
tcatcaccac agccaggatc cgatgaccac ctcaacgcaa acggaactta ag 52
<210> 213
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 213
gcattatgcg gccgcaagct tttaccattt tccatctccg at 42
<210> 214
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 214
tcatcaccac agccaggatc cgatggcata tcaggacgta tacgag 46
<210> 215
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 215
gcattatgcg gccgcaagct tttattggcg tagcccagcg attg 44
<210> 216
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 216
tcatcaccac agccaggatc cgatgaagaa ggttatcgtt ctgac 45
<210> 217
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 217
gcattatgcg gccgcaagct tttatttttt caagttcatt ag 42
<210> 218
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 218
tcatcaccac agccaggatc cgatgctgga aaaaggtagt acac 44
<210> 219
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 219
gcattatgcg gccgcaagct tttaaatgtt atttaaaaat tt 42
<210> 220
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 220
tcatcaccac agccaggatc cgatgaagaa ggttaatatt ctcac 45
<210> 221
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 221
gcattatgcg gccgcaagct tttatttctt caagttcatt agt 43
<210> 222
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 222
tcatcaccac agccaggatc cgatgaggaa ggttaagatt atgac 45
<210> 223
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 223
gcattatgcg gccgcaagct tttattctcc ctcgttgttt ttc 43
<210> 224
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 224
tcatcaccac agccaggatc cgatgagcac aagaaagaga cc 42
<210> 225
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 225
gcattatgcg gccgcaagct tttatttgtc ttcccaaaca tat 43
<210> 226
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 226
tcatcaccac agccaggatc cgatgattaa tagattattt tc 42
<210> 227
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 227
gcattatgcg gccgcaagct tttatttttg atcatcaaat aat 43
<210> 228
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 228
tcatcaccac agccaggatc cgatggaact aaacaatgtc atc 43
<210> 229
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 229
gcattatgcg gccgcaagct tttatctatt tttgaagcct tc 42
<210> 230
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 230
tcatcaccac agccaggatc cgaatagtaa aaaagtagtg atag 44
<210> 231
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 231
gcattatgcg gccgcaagct tttaaacgaa agctgacaat tcc 43
<210> 232
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 232
tcatcaccac agccaggatc cgatgggaaa tattatcttt gaag 44
<210> 233
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 233
gcattatgcg gccgcaagct tttaaagtcc tttgaagtct gc 42
<210> 234
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 234
tcatcaccac agccaggatc cgatggattt taataatatt atcc 44
<210> 235
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 235
gcattatgcg gccgcaagct tttatttatt cttaaaatta cc 42
<210> 236
<211> 49
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 236
tcatcaccac agccaggatc cgatggcact aagagatggg aattcctac 49
<210> 237
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 237
gcattatgcg gccgcaagct tttatttttc ttcctttatg ccg 43
<210> 238
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 238
tcatcaccac agccaggatc cgatgagatc aaaagaagat ttcc 44
<210> 239
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 239
gcattatgcg gccgcaagct tttaagctta ataccctttt a 41
<210> 240
<211> 47
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 240
tcatcaccac agccaggatc cgatgggact caaaacgaag gcggaat 47
<210> 241
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 241
gcattatgcg gccgcaagct tttacttcga ttcgattccc gcc 43
<210> 242
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 242
tcatcaccac agccaggatc cgatgatgac tagcgaacag tacg 44
<210> 243
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 243
gcattatgcg gccgcaagct tttaacttat cgagtgattc gtc 43
<210> 244
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 244
tcatcaccac agccaggatc cgatgagtca aagcacctcc c 41
<210> 245
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 245
gcattatgcg gccgcaagct tttaagttct tctgccaggc tc 42
<210> 246
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 246
tcatcaccac agccaggatc cgatggaatt tgaaacaata gaaac 45
<210> 247
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 247
gcattatgcg gccgcaagct tttaattttc ccttaaacgt aggc 44
<210> 248
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 248
tcatcaccac agccaggatc cgatggaaac aattgttata 40
<210> 249
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 249
gcattatgcg gccgcaagct tttatttacc cttaaactga gc 42
<210> 250
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 250
tcatcaccac agccaggatc cgatgaccga acaccagacc 40
<210> 251
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 251
gcattatgcg gccgcaagct tttagcggtg ggtgaagttc ggg 43
<210> 252
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 252
tcatcaccac agccaggatc cgatggcaga cagagtactc 40
<210> 253
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 253
gcattatgcg gccgcaagct tttaccggtt tcggtacttc gg 42
<210> 254
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 254
tcatcaccac agccaggatc cgatggaaac aattgttata aaaaaa 46
<210> 255
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 255
gcattatgcg gccgcaagct tttatttacc cttaaactga gcctt 45
<210> 256
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 256
tcatcaccac agccaggatc cggtgaccga acaccagacc atc 43
<210> 257
<211> 47
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 257
gcattatgcg gccgcaagct tttagcggtg ggtgaagttc gggtccc 47
<210> 258
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 258
tcatcaccac agccaggatc cgatggcaga cagagtactc atc 43
<210> 259
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 259
gcattatgcg gccgcaagct tttaccggtt tcggtacttc ggctc 45
<210> 260
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 260
tcatcaccac agccaggatc cggtggaatt tgaaaaaatt aaat 44
<210> 261
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 261
gcattatgcg gccgcaagct tttaccggcc tttaaaattc ggc 43
<210> 262
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 262
tcatcaccac agccaggatc cgatgagcgt gcgtgtcgag cgg 43
<210> 263
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 263
gcattatgcg gccgcaagct tttagcgccc gcgaaaagcc ggac 44
<210> 264
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 264
tcatcaccac agccaggatc cgatgaacgc tgacgccgag acc 43
<210> 265
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 265
gcattatgcg gccgcaagct tttagcgccc tttgaagttc ggc 43
<210> 266
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 266
tcatcaccac agccaggatc cgatgagtga tagaaataag 40
<210> 267
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 267
gcattatgcg gccgcaagct tttaattcag cctcctt 37
<210> 268
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 268
tcatcaccac agccaggatc cgatgtcaaa ttcagataaa t 41
<210> 269
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 269
gcattatgcg gccgcaagct tttaaaagtg tttctgaaaa agc 43
<210> 270
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 270
tcatcaccac agccaggatc cgatggcaga catttatact atg 43
<210> 271
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 271
gcattatgcg gccgcaagct tttaagctcc tttctttttg aaa 43
<210> 272
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 272
tcatcaccac agccaggatc cgatggaaaa caatacaaat a 41
<210> 273
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 273
gcattatgcg gccgcaagct tttatttttc cttccttacc 40
<210> 274
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 274
tcatcaccac agccaggatc cgatgtggca ttgtttagaa act 43
<210> 275
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 275
gcattatgcg gccgcaagct tttaaaccaa catgtctgca aa 42
<210> 276
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 276
tcatcaccac agccaggatc cggtggaaga agctaaaaaa caa 43
<210> 277
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 277
gcattatgcg gccgcaagct tttaaatttc cttatttgct tcc 43
<210> 278
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 278
tcatcaccac agccaggatc cgatgtacac attgggtgtt ga 42
<210> 279
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 279
gcattatgcg gccgcaagct tttataactt tttaaaaccg tat 43
<210> 280
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 280
tcatcaccac agccaggatc cgatgagcag tgtatacaca atggg 45
<210> 281
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 281
gcattatgcg gccgcaagct tttaagcgtt ttgcttctgg tat 43
<210> 282
<211> 48
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 282
tcatcaccac agccaggatc cgatgagtaa tatagatgta ttgttagg 48
<210> 283
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 283
gcattatgcg gccgcaagct tttaaatcat atcagcgaaa gtc 43
<210> 284
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 284
tcatcaccac agccaggatc cgatgagtaa tacaggaatg gt 42
<210> 285
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 285
gcattatgcg gccgcaagct tttagtgcgc ctccttcatc tt 42
<210> 286
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 286
tcatcaccac agccaggatc cgatgtctga aaaaaaagaa gcta 44
<210> 287
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 287
gcattatgcg gccgcaagct tttatatctc acctctatta 40
<210> 288
<211> 47
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 288
tcatcaccac agccaggatc cgatggaagc tattttatct aaaatga 47
<210> 289
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 289
gcattatgcg gccgcaagct tttactaaac tcatcatctc agc 43
<210> 290
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 290
tcatcaccac agccaggatc cgatgtacac aatgggatta ga 42
<210> 291
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 291
gcattatgcg gccgcaagct tttaattttt cacttctttt tg 42
<210> 292
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 292
tcatcaccac agccaggatc cgcttttaga aggagttaaa gtag 44
<210> 293
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 293
gcattatgcg gccgcaagct tttatcttac aactttacta tcttt 45
<210> 294
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 294
tcatcaccac agccaggatc cgatgagcga actgatcgtc ag 42
<210> 295
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 295
gcattatgcg gccgcaagct tttagcgtcc tttaaagtcg ggc 43
<210> 296
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 296
tcatcaccac agccaggatc cgatgagcta tcacacgatc cgc 43
<210> 297
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 297
gcattatgcg gccgcaagct tttaagcgcc cggtgaaatg cg 42
<210> 298
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 298
tcatcaccac agccaggatc cgatgacgga tgtcattcgg ctcg 44
<210> 299
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 299
gcattatgcg gccgcaagct tttattgccc ggcctg 36
<210> 300
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 300
tcatcaccac agccaggatc cgatgatcgt cggagtcatc gggtc 45
<210> 301
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 301
gcattatgcg gccgcaagct tttactcgtg gaggaacagc gcc 43
<210> 302
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 302
tcatcaccac agccaggatc cgatgttttc tattcaacaa gagg 44
<210> 303
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 303
gcattatgcg gccgcaagct tttattttcc ttgaaactgt ggtg 44
<210> 304
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 304
tcatcaccac agccaggatc cgatgagcac ggcgcccgaa gct 43
<210> 305
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 305
gcattatgcg gccgcaagct tttagcggcc gtgccagacc gg 42
<210> 306
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 306
tcatcaccac agccaggatc cggtggacaa tggccgtaag ctg 43
<210> 307
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 307
gcattatgcg gccgcaagct tttaatagtc ctcctttact ttg 43
<210> 308
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 308
tcatcaccac agccaggatc cggtgagcct ttcgccgctt gcc 43
<210> 309
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 309
gcattatgcg gccgcaagct tttaatgccc ggcgcggtac tgc 43
<210> 310
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 310
tcatcaccac agccaggatc cgatgtacaa attaatagat g 41
<210> 311
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 311
gcattatgcg gccgcaagct tttacttacc tttatactga gg 42
<210> 312
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 312
tcatcaccac agccaggatc cgatgtcaga aacaatcaat tt 42
<210> 313
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 313
gcattatgcg gccgcaagct tttaattttg tttcgttatt aac 43
<210> 314
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 314
tcatcaccac agccaggatc cggtgcaaga tgacagaagt t 41
<210> 315
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 315
gcattatgcg gccgcaagct tttattcacc ctttcttaat tt 42
<210> 316
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 316
tcatcaccac agccaggatc cgatgaaagg cagattagaa ga 42
<210> 317
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 317
gcattatgcg gccgcaagct tttataaaat atctttaaat gtt 43
<210> 318
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 318
tcatcaccac agccaggatc cgatgacgga tacaacaact at 42
<210> 319
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 319
gcattatgcg gccgcaagct tttaactcat tggaaactcc ctc 43
<210> 320
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 320
tcatcaccac agccaggatc cgatgagtag aattgaaacg att 43
<210> 321
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 321
gcattatgcg gccgcaagct tttaacagca tttctctaaa actt 44
<210> 322
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 322
tcatcaccac agccaggatc cgatgtttac aatggggatt g 41
<210> 323
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 323
gcattatgcg gccgcaagct tttatttatt agctccccta acttc 45
<210> 324
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 324
tcatcaccac agccaggatc cgatgaatac tatagatata tc 42
<210> 325
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 325
gcattatgcg gccgcaagct tttaacccct cattttttta ttgg 44
<210> 326
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 326
tcatcaccac agccaggatc cgatgtacac tatgggagta g 41
<210> 327
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 327
gcattatgcg gccgcaagct tttataaatt tttattcgcc tc 42
<210> 328
<211> 38
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 328
tcatcaccac agccaggatc cgatgaacaa aattaatg 38
<210> 329
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 329
gcattatgcg gccgcaagct tttaagagag tttctacaag agtt 44
<210> 330
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 330
tcatcaccac agccaggatc cgatgactga tacaacagct atg 43
<210> 331
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 331
gcattatgcg gccgcaagct tttactcatt ggaaactccc tcc 43
<210> 332
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 332
tcatcaccac agccaggatc cgagaagctg gaattaagag c 41
<210> 333
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 333
gcattatgcg gccgcaagct tttaacagca tttcactaaa ac 42
<210> 334
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 334
tcatcaccac agccaggatc cgatgtttac aatggggatt g 41
<210> 335
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 335
gcattatgcg gccgcaagct tttaatttat tagctcccct gac 43
<210> 336
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 336
tcatcaccac agccaggatc cgatgccaaa gacagtaagc cc 42
<210> 337
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 337
gcattatgcg gccgcaagct tttatttccc cttcttttca tc 42
<210> 338
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 338
tcatcaccac agccaggatc cgatggctat cagtgcactt attg 44
<210> 339
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 339
gcattatgcg gccgcaagct tttacaggct ttctgcgaaa gcttc 45
<210> 340
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 340
tcatcaccac agccaggatc cgatgagtat ctataccttg gg 42
<210> 341
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 341
gcattatgcg gccgcaagct tttatttggc tgctttttta tacg 44
<210> 342
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 342
tcatcaccac agccaggatc cgatgaaatt aaactatttt tgcag 45
<210> 343
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 343
gcattatgcg gccgcaagct tttacactcc acccacgctt tcc 43
<210> 344
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 344
tcatcaccac agccaggatc cgatggctaa aaaaatcttt aag 43
<210> 345
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 345
gcattatgcg gccgcaagct tttacaggct ctccaggaaa gcctg 45
<210> 346
<211> 49
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 346
tcatcaccac agccaggatc cgttgtatct tggagttgat attggttcg 49
<210> 347
<211> 47
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 347
gcattatgcg gccgcaagct tttaagaatt ttcagcggct aataaag 47
<210> 348
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 348
tcatcaccac agccaggatc cgatgaacaa tatttacacg atgggc 46
<210> 349
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 349
gcattatgcg gccgcaagct tttatttggt tgccttctgc catgc 45
<210> 350
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 350
tcatcaccac agccaggatc cgatggccga aaacgaaaaa gcc 43
<210> 351
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 351
gcattatgcg gccgcaagct tttatgcccg ggcctccttc tgc 43
<210> 352
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 352
tcatcaccac agccaggatc cgatgagtat cgaaacgatt g 41
<210> 353
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 353
gcattatgcg gccgcaagct tttaagaggg agatcacatc ggc 43
<210> 354
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 354
tcatcaccac agccaggatc cgatgactga tacagccaat atg 43
<210> 355
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 355
gcattatgcg gccgcaagct tttactcatg ggaaactccc tcc 43
<210> 356
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 356
tcatcaccac agccaggatc cgatgagtag aattgaagcg attatc 46
<210> 357
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 357
gcattatgcg gccgcaagct tttacagcat ttcacgaaaa c 41
<210> 358
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 358
tcatcaccac agccaggatc cgatgtatac tatggggatt gatatc 46
<210> 359
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 359
gcattatgcg gccgcaagct tttatacctc tttccttaat ttatt 45
<210> 360
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 360
tcatcaccac agccaggatc cgatgagtaa cacaggtgca gttg 44
<210> 361
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 361
gcattatgcg gccgcaagct tttagttttc ctccttcatc tttg 44
<210> 362
<211> 48
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 362
tcatcaccac agccaggatc cgatgagtaa cttagaagaa ctatttgg 48
<210> 363
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 363
gcattatgcg gccgcaagct tttatatcat gtctgcaaaa gtctg 45
<210> 364
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 364
tcatcaccac agccaggatc cgatgagtga tatatacaca atggg 45
<210> 365
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 365
gcattatgcg gccgcaagct tttaaatttt ccttcagata ctc 43
<210> 366
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 366
tcatcaccac agccaggatc cgatgagcga tgaaacgctt gtgc 44
<210> 367
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 367
gcattatgcg gccgcaagct tttagcgacc actgaagttg gctg 44
<210> 368
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 368
tcatcaccac agccaggatc cgatggaatt cataaaagta aacac 45
<210> 369
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 369
gcattatgcg gccgcaagct tttatttccc cttgaattca ggc 43
<210> 370
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 370
tcatcaccac agccaggatc cgatgtacga gaacctcatc g 41
<210> 371
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 371
gcattatgcg gccgcaagct tttacctccc tttccattcg ggc 43
<210> 372
<211> 47
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 372
tcatcaccac agccaggatc cgatgcaacc acaatttata atcatac 47
<210> 373
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 373
gcattatgcg gccgcaagct tttattttcc tttaaaaaca ggtg 44
<210> 374
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 374
tcatcaccac agccaggatc cggtgtatac tctcggaatc g 41
<210> 375
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 375
gcattatgcg gccgcaagct tttatttctt gattttatca taagc 45
<210> 376
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 376
tcatcaccac agccaggatc cgatgagtga agaaaaaaca gtag 44
<210> 377
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 377
gcattatgcg gccgcaagct tttagtggtt tcctccttca ttggc 45
<210> 378
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 378
tcatcaccac agccaggatc cgatgagtca gatcgacgaa cttatc 46
<210> 379
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 379
gcattatgcg gccgcaagct tttagaggat ttccgagaaa gcctg 45
<210> 380
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 380
tcatcaccac agccaggatc cgatgagtga agagtctctg g 41
<210> 381
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 381
gcattatgcg gccgcaagct tttaacgacc actgaagttg ggagc 45
<210> 382
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 382
tcatcaccac agccaggatc cgatgtctac aacacataac gtc 43
<210> 383
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 383
gcattatgcg gccgcaagct tttactcaac aggtaaggcg 40
<210> 384
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 384
tcatcaccac agccaggatc cgatgacaac aacagatctt gcgcc 45
<210> 385
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 385
gcattatgcg gccgcaagct tttacagagt cggcagtgcg c 41
<210> 386
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 386
tcatcaccac agccaggatc cgatgacgac agagcaaacc acgcc 45
<210> 387
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 387
gcattatgcg gccgcaagct tttacgccac aggcggcagc gg 42
<210> 388
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 388
tcatcaccac agccaggatc cgatgacaac aacgaataac actccc 46
<210> 389
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 389
gcattatgcg gccgcaagct tttaggccaa cgtggggatt ggac 44
<210> 390
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 390
tcatcaccac agccaggatc cgatggataa tactcctcag ggc 43
<210> 391
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 391
gcattatgcg gccgcaagct tttaaccctg aaccgggagc 40
<210> 392
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 392
tcatcaccac agccaggatc cgatgacaac aacgaataac accc 44
<210> 393
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 393
gcattatgcg gccgcaagct tttagacctg tttaggtatc ggac 44
<210> 394
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> primer: dhaB123_F
<400> 394
tcatgaaatc aaaaagattt gaagtattga ag 32
<210> 395
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> primer: dhaB123_R
<400> 395
ggatccctaa tcttttctaa gttgacctct ttgttc 36
<210> 396
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> primer: gdrAB_F
<400> 396
ggatccaaag gttcggggat agttatgaag 30
<210> 397
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> primer: gdrAB_R
<400> 397
gagctcttat ctaagtggca gaccctttac aag 33
<210> 398
<211> 1194
<212> DNA
<213> Cupriavidus necator succinate semialdehyde dehydrogenase (SSADH) protein gene: gabD gene
<400> 398
atgcacgccg ccacgcaagc catcctcacc ttcaaccatg gccgcgatcc cgagcggctg 60
acccgcaagc ttgcggcgat cgccgcggac ccgtttgcct tctttcgcgg caccaaccat 120
ctctatgccg catcgctgcg cgatgaggcg gcaatgtgca atgcgcccat cacctacgtc 180
tgcggcgatc tgcacctgga gaacttcggc agcttcaagg gcgacaacgg gctggtctat 240
ttcgacctga acgactttga cgatgccctg gtcgcgccgc ttacggtgga tgtggtccgg 300
atgctgtcga gcgtgctggt ggccgccggc cagctgggcc tttccgaggc cggcgccatg 360
cgcgcctgcg aggccatgct gtccacctat gccgccgtgc tgcagacagg caagcctcgc 420
tggctcgagc gtgccacggc ggtcggcatg gtggccaccc tgctgcgccg ggtcaagggc 480
cgcaagcgcg gcgcgctgct ggccgagctc accacgctgc gcaagggcaa acggcgcctg 540
gtatgcaacg gccgccatgc gctgccggcg gacaagccgg cccgtgagcg cgctcgcgcg 600
atccttgcgg cctactcgaa gcagggccac catggccacc gcctggccct cgacgatgcc 660
gcgcggcgcg tggcgggcat tggcagcctc gggctggaac gctatatggt gctggcgcgg 720
gacgaactga gcggcatgca gcgactggtc gacatcaagc gcgccgcgcc gagtccatgg 780
caggacctgc ccagcctgtc cctgccaccc tggggcagcg atgcaaggcg ggtggcggcg 840
gtgcagcaag tcatgcaggc ggcttcgccg gcgctgctgt cggctgtcga catgggcaag 900
gcttcctatc tggtcaagag cctgcagcca actgccgacc gcgtcgacct ggcgcattgc 960
agcaactttg cagcgctacg cgagttgctg ggcaccatgg cgcatgccgc ggcatgggcg 1020
catctgcgtg gctgcgggca ccaggccgcg gaccggatcg agcagctgca ggcgtttgcc 1080
ggcggcaccc gctggcgcac cggcgtcctg cggctggcac ggcatggctg cgcggtgtcg 1140
gtggtgcagt ggaaggcgta tgcggacgat taccgcgagg cgcggggagg gtga 1194
<210> 399
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> primer: gabD_F
<400> 399
aaagctagca tgtaccagga tctcgccc 28
<210> 400
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> primer: gabD_R
<400> 400
aatggtacct caggcctggg tgatgaactt 30
<210> 401
<211> 258
<212> PRT
<213> Ruegeria pomeroyi DSS-3
<400> 401
Met Ala Phe Glu Thr Ile Ile Val Glu Val Glu Asp His Val Ala Leu
1 5 10 15
Ile Arg Leu Asn Arg Pro Asp Ala Leu Asn Ala Leu Asn Thr Gln Leu
20 25 30
Leu Gly Glu Leu Cys Thr Ala Leu Glu Glu Ala Asp Gly Asn Asp Lys
35 40 45
Val Arg Cys Ile Val Ile Thr Gly Ser Asp Lys Ala Phe Ala Ala Gly
50 55 60
Ala Asp Ile Arg Glu Met Ser Gln Lys Thr Tyr Val Glu Val Tyr Ser
65 70 75 80
Glu Asn Leu Phe Ala Ala Ala Asn Asp Arg Val Ser Ala Ile Arg Lys
85 90 95
Pro Ile Ile Ala Ala Val Ala Gly Tyr Ala Leu Gly Gly Gly Cys Glu
100 105 110
Leu Ala Met Leu Cys Asp Phe Ile Ile Ala Ala Asp Thr Ala Lys Phe
115 120 125
Gly Gln Pro Glu Ile Asn Leu Gly Val Ile Ala Gly Ile Gly Gly Thr
130 135 140
Gln Arg Leu Thr Arg Leu Val Gly Lys Ser Lys Ser Met Asp Leu Asn
145 150 155 160
Leu Thr Gly Arg Phe Met Asp Ala Glu Glu Ala Glu Arg Ala Gly Leu
165 170 175
Val Ser Arg Val Val Pro Ala Lys Lys Leu Val Glu Glu Ala Leu Ser
180 185 190
Ala Ala Gln Lys Ile Ala Glu Lys Ser Met Ile Ser Ala Tyr Ala Val
195 200 205
Lys Glu Ala Val Asn Arg Ser Tyr Glu Thr Thr Leu Ser Glu Gly Leu
210 215 220
Leu Phe Glu Arg Arg Val Phe His Ser Met Phe Ala Thr Glu Asp Gln
225 230 235 240
Lys Glu Gly Met Ala Ala Phe Leu Glu Lys Arg Ala Ala Gln Phe Arg
245 250 255
Asp Lys
<210> 402
<211> 777
<212> DNA
<213> Ruegeria pomeroyi DSS-3
<400> 402
atggcctttg agacgatcat cgtcgaagtt gaagaccacg tagccctgat caggctgaac 60
cgtcccgatg cgctcaatgc gctcaacacc cagttgctgg gcgagttgtg taccgcgctg 120
gaagaggccg acggcaatga caaggtgcgc tgcatcgtca tcaccggcag cgacaaggca 180
tttgccgccg gggccgatat ccgcgagatg tcccaaaaga cctatgtcga ggtgtatagc 240
gagaacctgt tcgcggccgc caacgaccgt gtcagcgcca tccgcaagcc gatcatcgcc 300
gcagtggcgg gctatgcgct gggcggtggc tgtgaactgg cgatgctgtg cgatttcatc 360
atcgcggcgg acaccgcaaa gttcggccag cccgagatca acctgggcgt gatcgccggt 420
atcggcggca cccagcgtct gacccggctg gtgggcaagt ccaagtcgat ggacctgaac 480
ctgaccgggc ggttcatgga tgccgaagag gccgagcgcg ccgggctggt cagccgcgtg 540
gttccggcca agaagctggt cgaagaggcg ctgagcgcag cccagaagat cgccgagaaa 600
tcgatgatct cggcctatgc ggtcaaggag gcggtcaacc gctcttacga gaccacgctg 660
agcgaggggc tgctgttcga gcgccgggtg ttccattcga tgttcgccac cgaagatcag 720
aaggaaggca tggccgcttt cctcgagaag cgggcggcac agttccgcga caagtga 777
<210> 403
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 403
tcatcaccac agccaggatc cgatggcctt tgagacgatc atcg 44
<210> 404
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> primer
<400> 404
gcattatgcg gccgcaagct tttacttgtc gcggaactgt gccgc 45
Claims (20)
- 유전적으로 조작되지 않은 세포에 비하여 3-히드록시프로피온산 (3-HP)을 3-히드록시프로피오닐-CoA (3-HP-CoA)로 전환하는 것을 촉매하는 CoA 트랜스페라제 (CoA transferase), 3-HP-CoA를 아크릴릴-CoA (acrylyl-CoA)로 전환하는 것을 촉매하는 3-HP-CoA 데히드라타제, 및 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소의 활성이 증가되어 있는, 아크릴레이트 생산능을 갖는 미생물로서,
상기 미생물은 CoA 트랜스페라제, 3-HP-CoA 데히드라타제, 및 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소를 코딩하는 외인성 폴리뉴클레오티드가 도입된 것이고,
상기 CoA 트랜스페라제는 3-HP를 3-HP-CoA로 전환하는 것을 촉매하는 활성이 그 역반응을 촉매하는 것보다 높은 것이고,
상기 3-HP-CoA 데히드라타제는 3-HP-CoA를 아크릴릴-CoA로 전환하는 것을 촉매하는 활성이 그 역반응을 촉매하는 것보다 높은 것이고,
상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 활성이 그 역반응을 촉매하는 것보다 높은 것이고,
상기 미생물은 글리세롤을 3-프로피온산 알데히드 (3-propionic aldehyde: 3-HPA)로 전환하는 반응을 촉매하는 효소를 코딩하는 폴리뉴클레오티드, 및 3-HPA를 3-HP로 전환하는 반응을 촉매하는 효소를 코딩하는 폴리뉴클레오티드가 도입되어 있어, 3-HP 생산능을 갖는 것인 아크릴레이트 생산능을 갖는 미생물. - 청구항 1에 있어서, 상기 CoA 트랜스페라제는 서열번호 1 내지 10의 아미노산 서열로 이루어진 군으로부터 선택된 하나 이상을 갖는 것인 미생물.
- 청구항 1에 있어서, 상기 CoA 트랜스페라제는 EC 2.8.3.8, EC 3.1.2.-, 또는 EC 6.2.1.7에 속하는 것인 미생물.
- 청구항 1에 있어서, 상기 3-HP-CoA 데히드라타제는 서열번호 21 내지 98, 및 401의 아미노산 서열로 이루어진 군으로부터 선택된 하나 이상을 갖는 것인 미생물.
- 청구항 1에 있어서, 상기 3-HP-CoA 데히드라타제는 EC 4.2.1.17, EC 4.2.1.55, 및 EC 4.2.1.166를 포함한, EC 4.2.1.-에 속하는 것인 미생물.
- 청구항 1에 있어서, 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 서열번호 177 내지 182의 아미노산 서열로 이루어진 군으로부터 선택된 하나 이상을 갖는 것인 미생물.
- 청구항 1에 있어서, 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 EC 3.1.2.4를 포함한, EC 3.1.2-에 속하는 것인 미생물.
- 청구항 1에 있어서, 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 3-HP-CoA 히드롤라제 (3-HP-CoA hydrolase), 또는 3-히드록시이소부티릴-CoA 히드롤라제 (3-hydroxyisobutyryl-CoA hydrolase)인 것인 미생물.
- 청구항 1에 있어서, CoA 트랜스페라제, 3-HP-CoA 데히드라타제, 및 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소의 활성의 증가는 이들 효소를 코딩하는 폴리뉴클레오티드의 발현 증가에 의한 것인 미생물.
- 삭제
- 청구항 1에 있어서, 상기 미생물은 에세리키아 (Esherichia), 루멘박테리아,
코리네박테리움 (Corynebacterium) 속 및 브레비박테리움 (Brevibacterium) 속으로
구성된 군으로부터 선택되는 것인 미생물. - 청구항 1에 있어서, 상기 미생물은 아크릴레이트를 분해하거나 다른 산물로 전환하는 경로에 관여하는 하나 이상의 효소의 활성이 감소된 것인 미생물.
- 청구항 1에 있어서, 상기 미생물은 아크릴레이트를 분해하거나 다른 산물로 전환하는 경로에 관여하는 하나 이상의 효소를 코딩하는 유전자가 제거 또는 파괴되어 있는 것인 미생물.
- 삭제
- 청구항 1에 있어서, 상기 미생물은 대장균인 것인 미생물.
- 청구항 1에 있어서, 글리세롤을 3-HPA로 전환하는 반응을 촉매하는 효소는 dhaB이고, 3-HPA를 3-HP로 전환하는 반응을 촉매하는 효소는 AldH 또는 gabD인 것인 미생물.
- 청구항 1의 미생물을 배지 중에서 배양하는 단계;를 포함하는, 아크릴레이트를 생산하는 방법.
- 청구항 17에 있어서, 배양물로부터 아크릴레이트를 회수하는 단계를 더 포함하는 것인 방법.
- 청구항 17에 있어서, 상기 미생물은 아크릴레이트를 다른 산물로 전환하는 경로를 더 포함하는 것이고, 생산된 아크릴레이트를 다른 산물로 전환하는 단계를 더 포함하는 것인 방법.
- 청구항 19에 있어서, 다른 산물은 아크릴레이트 에스테르인 것인 방법.
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| US14/620,049 US9506089B2 (en) | 2014-05-14 | 2015-02-11 | Microorganism having novel acrylic acid synthesis pathway and method of producing acrylic acid by using the microorganism |
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| KR20160006030A (ko) * | 2014-07-08 | 2016-01-18 | 삼성전자주식회사 | CoA 아실화 알데히드 데히드게나제 활성이 증가된 신규한 아크릴산 생성 경로를 갖는 미생물 및 이를 이용한 아크릴산 생산 방법 |
| KR102173569B1 (ko) * | 2016-01-18 | 2020-11-04 | 한화솔루션 주식회사 | 아크릴산 생성능을 가지는 재조합 변이 미생물 및 이를 이용한 아크릴산의 제조방법 |
| CN111363713A (zh) * | 2020-03-24 | 2020-07-03 | 华东理工大学 | 一种提高聚羟基丁酸乳酸酯中乳酸组分含量的基因工程大肠杆菌的构建方法及应用 |
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| CN1556855A (zh) | 2000-11-20 | 2004-12-22 | 卡吉尔公司 | 3-羟基丙酸及其它有机化合物 |
| DE102005048818A1 (de) | 2005-10-10 | 2007-04-12 | Degussa Ag | Mikrobiologische Herstellung von 3-Hydroxypropionsäure |
| DE102006039203B4 (de) | 2006-08-22 | 2014-06-18 | Evonik Degussa Gmbh | Verfahren zur Herstellung von durch Kristallisation gereinigter Acrylsäure aus Hydroxypropionsäure sowie Vorrichtung dazu |
| US8048624B1 (en) | 2007-12-04 | 2011-11-01 | Opx Biotechnologies, Inc. | Compositions and methods for 3-hydroxypropionate bio-production from biomass |
| EP2304039B1 (en) * | 2008-06-17 | 2019-08-21 | Genomatica, Inc. | Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate |
| US20140342414A1 (en) * | 2011-09-22 | 2014-11-20 | Codexis, Inc. | Direct biocatalytic production of acrylic acid and other carboxylic acid compounds |
| WO2013192451A1 (en) | 2012-06-20 | 2013-12-27 | Opx Biotechnologies, Inc. | Dehydration of 3-hydroxypropionic acid to acrylic acid |
| KR20140003262A (ko) * | 2012-06-29 | 2014-01-09 | 삼성전자주식회사 | 재조합 미생물을 이용한 3-하이드록시프로피온산의 생산방법 및 그에 사용되는 재조합 미생물 |
| US20140099676A1 (en) * | 2012-10-05 | 2014-04-10 | Jun Xu | Microorganisms and methods for producing acrylate and other products from homoserine |
| KR20150110144A (ko) * | 2014-03-24 | 2015-10-02 | 삼성전자주식회사 | 글리세롤, 3-hp, 또는 아크릴산 생산능을 갖는 재조합 미생물 및 그를 이용하여 글리세롤, 3-hp, 또는 아크릴산을 생산하는 방법 |
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| WO2009089457A1 (en) * | 2008-01-11 | 2009-07-16 | Novozymes A/S | Methods for producing 3-hydroxypropionic acid and compounds thereof |
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