KR102206481B1 - Temperature stable composition for controlling pests and method for controlling pests using the same - Google Patents

Abstract

The present invention relates to a pest control composition capable of stable storage for a long period at a high temperature of 50° C. or higher as well as at room temperature, and a pest control method using the same.

Description

Temperature stable composition for controlling pests and method for controlling pests using the same {Temperature stable composition for controlling pests and method for controlling pests using the same}

The present invention relates to a pest control composition capable of stable storage at room temperature as well as high temperature for a long period of time, and a pest control method using the same.

Unlike chemical pesticides, microbial crop protection agents that control pests using pathogenic fungi have the advantage of minimizing pesticide resistance and environmental pollution because they use fungal spores, but important matters for commercialization, mass production and stabilized formulation Research is still insufficient. In particular, spores of pathogenic fungi must be prepared in the form of liquid formulations for spray application, but when mixed with water, spores die within one week, and in powder form, all spores die within one week when exposed to high temperatures. . It is common for crop protection agents to be exposed to temperatures above 40℃ during distribution.

Due to this problem, it is difficult for the conventional insect pathogenic fungi formulation to be stored in a stable manner for a long period of time, and it is difficult to export and sell to other countries, so a large ripple effect is expected when storage stability is improved.

In this regard, in the JP4581410 patent, insecticidal filamentous fungi are dispersed in petroleum hydrocarbon oil containing an emulsifier to improve mitigation and insecticidal effect. However, it does not mention anything about storage stability. In addition, U.S. Patent No. 5512280 improves long-term storage stability at room temperature by using an emulsifier, but does not disclose or imply a matter on high temperature stability. In the US1996/017950 patent, room temperature stability was improved by reducing oxygen exposure. Furthermore, in the US2013/0203154 patent, oxygen and moisture were excluded using gas and water vapor impermeable packaging, resulting in improved long-term storage stability at high temperatures (50°C). US Patent Publication No. 2007/0141032 discloses a technology for improving storage stability by using a petroleum hydrocarbon oil, a nonionic surfactant suitable for emulsification of petroleum hydrocarbon oil, and a granule-sized dehydrating agent.

Japanese Patent Registration No. 4581410 US Patent Registration No. 5512280 US Patent Publication No. 1996/017950 US Patent Publication No. 2013/0203154 US Patent Publication No. 2007/0141032

Under this background, the present invention solves the problem that the stability of pathogenic fungi spores is impaired by factors such as moisture or temperature, and provides a pest control composition and a pest control method capable of stable storage for a long time not only at room temperature but also at high temperature. .

For example, it provides a composition for controlling pests, including pathogenic fungi or spores thereof, a porous inorganic material having a pore size of 3 to 5Å, an oily solvent, and a surfactant.

In another example, it provides a pest control method comprising the step of applying the composition for controlling pests to pests, habitats of pests, or crops vulnerable to pests.

As another example, it provides a method for stabilizing a composition for controlling pests, including the step of mixing and storing a pathogenic fungus or its spores, a porous material having a pore size of 3 to 5Å, an oily solvent, and a surfactant.

In the present invention, by mixing pathogenic fungi or spores thereof with a porous inorganic material having a pore size of 3 to 5 Å, an oily solvent, and a surfactant, a composition for controlling pests capable of stable storage for a long period of time not only at room temperature but also at high temperature and use thereof is provided.

According to one aspect of the present invention, there is provided a composition for controlling pests, including pathogenic fungi or spores thereof, a porous inorganic material having a pore size of 3 to 5 Å, an oily solvent, and a surfactant.

In accordance with another aspect of the present invention, a pest control method is provided, comprising applying the composition for controlling pests to pests, habitats of pests, or crops susceptible to pests.

According to another aspect of the present invention, there is provided a method for stabilizing a composition for controlling pests, comprising the step of mixing and storing a pathogenic fungus or its spores, a porous inorganic material having a pore size of 3 to 5Å, an oily solvent, and a surfactant. .

As used herein, "pathogenic fungi" refers to fungi that cause disease in organisms and mainly proliferate through spores. The "entomopathogenic fungi", which is a preferred example of pathogenic fungi to which the present invention is applicable, mainly belongs to imperfect fungi and conjugate fungi, taxonomically, and refers to fungi having pest control activity. Insect pathogenic fungi are different from insect pathogenic bacteria or viruses that are infected through host feeding, after fungal spores adhere to the outer shell of host insects and germinate, then pass through the cuticle layer or germinated from the spores with an invasion tool. It directly passes through the cuticle layer and invades the inside of the insect. At this time, the physical action and enzyme actions such as chitinase, protease, lipase, and esterase occur together. Fungi that invade the insect's body can be used as insect quarantine insecticides because they secrete toxic substances while proliferating in the insect's body to block the insect's immune function and kill host insects.

More than 700 species of insect pathogenic fungi are currently reported, and any one having an insecticidal effect on insects by generating spores can be used without limitation. For example, the beam Beria (Beauveria) in director Liao (Isaria), A Greca nisil Solarium (Lecanicillium) in ah (Nomuraea), A celecoxib Sat tree glutamicum (Celletotrichum) genus Fusarium (Fusarium) to Nomura in blood Saratov Tora (Phytophthora), A's Klee Loti California (Sclerotinia), Metairie Clear (Metarhizium), A par indeed My process (Paecilomyces), A tying yarn Solarium (Verticillium) genus Trichoderma (Trichoderma), A heosyu telra (Hirsutella ), Aspergillus , Aschersonia , or The fungi of the genus Metarhizium can be exemplified. Specifically, Beauveria bassiana , Metarhizium anisoplia anisopliae ), Verticillium lecanii ), Aschersonia aleyrodis or Nomurae relay ( Nomuraea rileyi ) can be illustrated. The boberia bassiana used in an example of the present application is a strain having excellent insecticidal effect against saw-legged ants, yellow flower thrips, and the like.

Such insect pathogenic fungal spores may be appropriately included in an amount capable of exhibiting an insecticidal effect, but preferably, 0.1 to 90% by weight, 0.1 to 50% by weight, 0.1 to 30% by weight, 0.1 to It may be 20% by weight, 0.5 to 20% by weight, or 1 to 15% by weight, but is not limited thereto. However, if it is less than the above range, there may be a problem that the insecticidal effect may not be sufficient due to the spore concentration below the effective concentration when used, and if it exceeds the above range, there may be a problem in the stability during storage.

Spores are germ cells produced by fungi for asexual reproduction, and can develop into adults without forming a zygote, and preferably include conidia. The composition for controlling pests of the present application includes insect pathogenic fungi or spores thereof, which is understood to include spores, hyphae, mycelium, fruiting bodies, spores or their equivalents. When the spores touch the insect's shell, a germination tube is created from the spore, and the germination tube grows until the nutrients in the spore are depleted, and begins to penetrate the cuticle layer of the insect, and the germination tube penetrates the enzyme that dissolves the cuticle layer. Secrete.

The composition for controlling pests of the present invention is characterized in that it is a porous inorganic material having a pore size of 3 to 5 Å as an additive, particularly in order to solve the temperature stability problem. These inorganic materials have a porous structure comprising ordered or disordered pores having a pore size of 3 to 5 Å. The shape and shape of the pores may vary in a spherical shape, a channel shape, a slit shape, a hexagonal shape, and the like, and the inorganic material may have various shapes and particle diameters such as a powder shape, a rod shape, a sphere shape, a tube shape, or a cylinder shape. As such an inorganic material, for example, alumino silicate, alumino phosphate, silica gel, alumina, aerogel, zirconia, calcium oxide, zinc oxide, titanium oxide, etc. may be used alone or in combination, but is not limited thereto. More specifically, As an example of an inorganic material having a porosity of 3 to 5Å, a zeolite corresponding to crystalline alumina silicate may be used, and zeolite 3A, zeolite 4A, or zeolite 5A may be exemplified.

Zeolite, an example of the inorganic material, is a crystalline aluminosilicate in which a plurality of pores are regularly formed. Zeolite is an inorganic polymer material formed by three-dimensionally connecting silicon (Si) and aluminum (Al) through an oxygen atom, and has various shapes and sizes of 3 Å to 100 Å (0.3 nm to 10 nm) depending on the type. It is structurally characterized by having a nanopore, and about 35 species are currently known. Among them, for example, zeolite 3A having a pore size of about 3Å, zeolite 4A having a pore size of about 4Å, or zeolite 5A having a pore size of about 5Å are suitable for the present application.

In the present application, the porous inorganic material having a pore size of 3 to 5 Å lowers the moisture content in the composition, thereby contributing to the long-term stability of the pest control composition. In particular, in the case of high temperature, it was confirmed that the porous inorganic material of the present application effectively absorbs moisture and secures stability in an environment where the influence of moisture is greater than that of room temperature, thereby maintaining stability even at high temperatures. When the pore size is less than 3 Å, moisture is not absorbed by the porous inorganic material, and when the pore size exceeds 5 Å, not only the moisture but also the proportion of other absorbed materials increases, so that moisture cannot be effectively absorbed.

The inorganic material is 0.1 to 90% by weight, 0.1 to 50% by weight, 0.1 to 30% by weight, 0.1 to 20% by weight, 0.5 to 20% by weight, or 1 to 15% by weight, based on 100% by weight of the composition for controlling pests It may be, but is not limited thereto. However, if it is less than the above range, there may be a problem in that sufficient moisture cannot be adsorbed.

The composition for controlling pests of the present invention may also contain a surfactant. Surfactants are amphiphilic substances having both a hydrophilic molecular group and a lipophilic molecular group in a molecule, and have cleaning power, dispersing power, emulsifying power, solubilizing power, wetting power, sterilizing power, foaming power and/or penetrating power, and for controlling pests according to the present invention. Pathogenic fungi or spores in the composition may act to hydrate, suspend or disperse to effectively exert a pest control effect.

Surfactants include, by way of non-limiting examples, hydrophilic surfactants, lipophilic surfactants, or mixtures thereof. For example, the hydrophilic lipophilic balance (HLB) value of the surfactant may be about 1 to 20, or about 1 to 17. In one embodiment, the HLB of the surfactant may be 1 to 7, or 10 to 17, or 10 to 20. In another embodiment, the HLB of the surfactant may be 1 or more to less than 7, 10 to less than 17, or 10 to less than 20. The HLB range exemplifies a suitable range in which a surfactant can effectively dissolve in an oily solvent and exhibit a surfactant effect, but the scope of the present invention is not limited thereto. HLB is an index indicating the balance between hydrophilicity and lipophilicity (hydrophobicity) of a surfactant, and it is understood that the greater the HLB, the greater the proportion of hydrophilicity, and the smaller the HLB, the greater the proportion of lipophilic (hydrophobicity). The surfactant may also be nonionic, anionic, cationic, or zwitterionic (or amphoteric).

Examples of cationic surfactants that can be used herein include DTAB (Dodecyltrimethylammonium bromide) or CTAB (Cetyltrimethylammonium bromide), and examples of anionic surfactants include DSS (dioctyl sulfosuccinate sodium), SDS (sodium dodecyl sulfate), LDS (lithium dodecyl sulfate), sodium dodecyl benzene sulfonate (SDBS), sodium dodecylsulfonate (SDSA), and the like. Examples of amphoteric surfactants include lecithin, and examples of nonionic surfactants include La. Usyl PEG/PPG-18/18 Methicone, Cetyl Diglyceryl Tris(Trimethylsiloxy)silylethyl Dimethicone, PEG-10 Dimethicone (PEG-10 dimethicone), Sorbitan monooleate, Octylphenol Ethoxylate, polyethylene glycol, alkyl polyglucoside, silane polyalkylene oxide (copolymer), nonylphenol ethoxylate Rate, tristyrylphenol ethoxylate, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, alkylallyl formaldehyde condensed polyoxyethylene ether, polyoxyethylene polyoxypropylene block polymer, polyoxyethylene polyoxypropylene alkyl Ether, polyoxyethylene ether of glycerin ester, polyoxyethylene ether of sorbitan ester, polyoxyethylene ether of sorbitol ester, polyethylene glycol fatty acid ester, glycerin ester, polyglycerin ester, sorbitan ester, propylene glycol ester, sucrose ester , Aliphatic acid alkanol amide, polyoxyethylene fatty acid amide, alcohol All ethoxylate, amine ethoxylate, glucoside, glucamide, polyethylene glycol, poly(ethylene glycol-co-propylene glycol), cetyl alcohol, stearyl alcohol, cetostearyl alcohol, oleyl alcohol, octaethylene glycol mono Dodecyl ether, pentaethylene glycol monododecyl ether, polyoxypropylene glycol alkyl ether, decyl glucoside, lauryl glucoside, octyl glucoside, polyoxyethylene glycol octylphenol ether, nonoxynol-9, glycerol alkyl ester, glyceryl laur Rate, polyoxyethylene glycol sorbitan alkyl ester, polysorbate, sorbitan alkyl ester, span, cocamide MEA, cocamide DEA, dodecyldimethylamine oxide, block copolymer of polyethylene glycol, polypropylene glycol , And silane polyalkylene oxide (copolymer), or polyoxyethylene alkyl amide, for example, Triton (registered trademark) X-114, X-102, X-100, X-45, X-15 , BG-10 or CG-119, or BRIJ (registered trademark) polyoxyethylene-4-lauryl ether (brij30), polyoxyethylene-23-lauryl ether (brij35), polyoxyethylene-2-cetyl ether ( brij52), polyoxyethylene-10-cetyl ether (brij56), polyoxyethylene-20-cetyl ether (brij58), polyoxyethylene-2-stearyl ether (brij72), polyoxyethylene-10-stearyl ether ( brij76), polyoxyethylene-2-oleyl ether (brij93) or polyoxyethylene-10-oleyl ether (brij97), PEG400 dioleate, and the like, but are not limited thereto. .

In one embodiment of the present application, the surfactant is DSS (dioctyl sulfosuccinate sodium), lauryl PEG/PPG-18/18 methicone, cetyl diglyceryl tris (trimethylsiloxy) silylethyl dimethicone, PEG-10 dimethicone, It may be one or more selected from the group consisting of sorbitan monooleate, hexadecyl trimethyl ammonium bromide, octylphenol ethoxylate, polyoxyethylene-2-oleyl ether, lecithin and PEG400 dioleate, but is not limited thereto. .

Surfactant is 0.1 to 90% by weight, 0.1 to 50% by weight, 0.1 to 30% by weight, 0.1 to 20% by weight, 0.5 to 20% by weight, or 1 to 15% by weight, based on 100% by weight of the composition for controlling pests However, it is not limited thereto. However, if it is less than the above range, there may be a problem that the surfactant effect does not appear sufficiently, and if it exceeds the above range, there may be a problem that the surfactant does not dissolve in the solvent and precipitation or precipitation occurs.

In addition, the composition for controlling pests of the present invention may also contain an oily solvent. Oily solvents refer to fatty substances that are liquid at ambient temperature (about 20-25° C.), or fatty solvents comprising oils of vegetable, mineral, animal or synthetic origin or mixtures thereof, which may be volatile or non-volatile. In the present invention, the stability against moisture may be improved by using an oily solvent as a storage medium in order to prevent spores from being exposed to moisture as much as possible by confirming the tendency of spores to be unstable to moisture. The oily solvent preferably has a water content of less than 0.1% by weight, and non-limiting examples thereof include, specifically, mineral oil, paraffin oil, silicone oil, vegetable oil (for example, sunflower, corn, soybean, pumpkin seed, grape seed, Olive, sesame, cotton seed, hazelnut, apricot, macadamia, coriander, castor or avocado oil, triglycerides of caprylic/capric acid, etc.), fish oil, and animal fat oils. However, it is not limited thereto.

The oily solvent is 60 to 95% by weight, 60 to 90% by weight, 70 to 95% by weight, 70 to 90% by weight, 80 to 95% by weight, or 80 to 90% by weight, based on 100% by weight of the pest control composition, It may be, but is not limited thereto.

The composition for controlling pests according to the present invention may be prepared according to a known method for preparing a composition for controlling pests. For example, it can be prepared by combining a pathogenic fungus or spores thereof, a porous material having a pore size of 3 to 5 Å, an oily solvent, and a surfactant. Pathogenic fungi or spores thereof can be pulverized to have an appropriate particle size if necessary, and after preparing a liquid sample by adding a dispersant and a porous material to an oily solvent, the pulverized pathogenic spores are mixed to control pests according to the present invention. A composition for use can be prepared. The composition for controlling pests according to the present invention may be formulated in various forms, but a liquid concentrate form capable of spraying is preferred.

The composition for controlling pests according to the present invention exhibits excellent stability not only at room temperature but also at high temperature. Specifically, the composition for controlling pests according to the present invention is at 50°C, or 54°C or higher, for example, at 50°C to 60°C, 50°C to 55°C, or 50°C to 54°C, the insect pathogenic fungus or More than 1.0% of its spores can survive for over a week. In addition, at room temperature, or 50°C, or 54°C or more, for example, 50°C to 60°C, 50°C to 55°C, or 50°C to 54°C, for 1 week or more, preferably 5 to 6 weeks. It is characterized by maintaining pathogenicity for more than a week.

The composition of the present invention may be used in a method for controlling pests by applying the composition for controlling pests according to the present invention to pests, habitats of pests, or crops susceptible to pests.

Examples of pests controllable by the composition of this embodiment include arthropods such as insects and ticks, especially harmful arthropods such as harmful insects and harmful mites, and the following can be exemplified:

- half bandwagon (Hemiptera): myeolguryu (Delphacidae), for example aemyeolgu (Laodelphax striatellus), Brown Planthopper (Nilaparvata 　 lugens ) and whiteback ( Sogatella furcifera ); Cicada (Deltocephalidae), such as the cicada ( Nephotettix cincticeps ) and two- pointed cicadas ( Nephotettix virescens ); Aphids (Aphididae), such as cotton aphids ( Aphis gossypii ), peach aphids ( Myzus persicae ), cabbage powder aphid ( Brevicoryne brassicae ), potato beard aphid ( Macrosiphum euphorbiae ), Aphid aphid ( Aulacorthum 　 solani ), millet border aphid ( Rhopalosiphum padi ) and tangerine aphid ( Toxoptera citricidus ); Pentatomidae, such as Nezara antennata , Saw-legged ant, Riptortus clavetus ), Leptocorisa chinensis , Eysarcoris parvus ), Halyomorpha mista and blind stinkwood ( Lyuslineolaris ); Flour ( Aleyrodidae ), such as greenhouse powder ( Taleurodes) vaporariorum), cigarettes Louis (Bemisiatabaci) and silver leaf whitefly (Bemisia argentifolii ); Pods worms current (Coccidae), such as California red worms pods (Aonidiella aurantii ), San Jose snapworm ( Comstockaspis perniciosa ), arrowhead bug ( Unaspiscitri ), ruby snapworm ( Ceroplastes rubens ), and Iseria snapworm ( Icerya purchasi ); Shield bugs (Tingidae); Psyllidae , etc.;

-Lepidoptera : Pyralidae , such as Chilo suppressalis , Yellow rice moth ( Tryporyzaincertulas ), Cnaphalocrocrocis medinalis , Cotton moth ( Notarchaderogata ), Hwaranggok moth ( Plodia interpunctella ) Moth ( Ostrinia 　 furnacalis ), European lighting moth ( Ostrinia nubilaris ), Chinese cabbage sprout moth ( Hellula 　 undalis ) and grass insect moth ( Pediasiateterrellus ); Chestnut moths ( Noctuidae ), such as tobacco casters ( Spodoptera litura ), chestnut moths ( Spodoptera exigua ), Pseudaletia separata , Thief moth ( Mamestra brassicae ), Arrest Seminar ( Agrotis ipsilon ), Street Cabbage Gold Winged Chestnut Butterfly ( Plusianigrisigna ), Thoricoplusia spp., Tobacco Chestnut Moth ( Heliothis spp.) and Tobacco Moth ( Helicoverpa spp.); White butterfly ( Pieridae ), such as Chinese cabbage white butterfly ( Pierisrapae ); Tortricidae , for example, Adoxophyes spp., Peach sprout moth ( Grapholitamolesta ), Bean moth ( Leguminivora) glycinivorella ), red bean moth ( Matsumuraeses azukivora ), apple leaf moth ( Adoxophyesorana) 　 fasciata ), Adoxophyes honmai ), tea leaf moth ( Homona 　 magnanima), geommo pattern ipmalyi moth (Archipsfuscocupreanus) and code Lin moth (Cydia 　 pomonella ); Fine moth ( Gracillariidae ), such as Camellia fine moth ( Caloptilia 　 theivora ) and apple oyster moth ( Phyllonorycter) ringoniella ); Heart-eating moth ( Carposinidae ), such as peach heart-eating moth ( Carposina niponensis ); Oyster moth ( Lyonetiidae ), such as silver pattern oyster moth ( Lyonetia spp.); Poisonous moths ( Lymantriidae ), such as gypsy moths ( Lymantria spp.) and poisonous moths ( Euproctis spp.); House moth ( Yponomeutidae ), such as Chinese cabbage moth ( Plutella xylostella ); Horned moth (Gelechiidae), such as cotton sputum moth ( Pectinophora gossypiella ) and potato horn moth ( Phthorimaea 　 operculella ); Fire moth ( Arctiidae ), such as American white fire moth ( Hyphantria 　 cunea ); And grain moths ( Tineidae ), such as Tinea translucens ;

-Thysanoptera: flower yellow thrips ( Frankliniella occidentalis ), cucumber thrips ( Thrips) 　 palmi ), convex thrips ( Scirtothrips dorsalis ), thrips thrips ( Thripstabaci ), Taiwanese thrips ( Frankliniella intonsa ) and tobacco thrips ( Frankliniella fusca ), etc.;

-Diptera: oyster flies ( Agromyzidae ), such as oyster flies ( Hylemyaantiqua ), scabies flies ( Hylemyaplatura ), rice leaf oyster flies ( Agromyza oryzae ), rice leaf oyster flies ( Hydrellia 　 griseola ), Chlorops oryzae ), American leaf oyster fly ( Liriomyza 　 trifolii ); Melon fly ( Dacus cucurbitae ) and Mediterranean fruit fly ( Ceratitis capitata ), etc.;

- second bandwagon (Coleoptera): 28-spotted ladybird (Epilachna vigintioctopunctata), cucumber beetle (Aulacophora 　 femoralis ), flea leaf beetle ( Phyllotreta striolata ), rice leaf beetle ( Oulema 　 oryzae ), Rice Root Weevil ( Echinocnemus squameus ), Rice Weevil ( Lissorhoptrus 　 oryzophilus ), Cotton weevil ( Anthonomus grandis ), Red weevil ( Callosobruchus 　 chinensis ), Hunting Weevil ( Sphenophorus venatus ), Japanese Bean Beetle ( Popillia japonica ), Copper Beetle ( Anomala cuprea ), corn leaf beetle ( Diabrotica spp.), Colorado potato leaf beetle ( Leptinotarsa . decemlineata), skipjack acids (Agriotes spp), etc. and the cigarette beetle (Lasioderma serricorne);

-Grasshopper (Orthoptera): Land-dog ( Gryllotalpa africana ), rice grasshopper ( Oxya yezoensis ) and rice grasshopper ( Oxya japonica ), etc.;

-Hymenoptera : Leafless Bee ( Athalia rosae ), scissors ants (workers) ( Acromyrmex spp.) and fire ants ( Solenopsis spp.), etc.;

-Mites (Acarina): mites (Tetranychidae), such as spotted mites ( Tetranychus urticae ), tangerine mites ( Panonychus 　 citri ) and tree mite ( Oligonychus spp.); Hawke Mites (Eriophyidae), for example gyulnok mite (Aculops pelekassi); Dust mites ( Tarsonemidae ), such as tea dust mites ( Polyphagotarsonemus latus ); Earth mite ( Tenuipalpidae ); Chire Mites (tuckerellidae); Dusty mites ( Acaridae ), such as long-haired dusty mites ( Tyrophagus 　 putrescentiae ); Dust mites ( Pyroglyphidae ), such as large leg dust mites ( Dermatophagoides 　 farinae ) and vertical pattern dust mites ( Dermatophagoides ptrenyssnus ); Claw mites ( Cheyletidae ), such as short comb mites ( Cheyletus 　 eruditus ), toenail mite ( Cheyletusmalaccensis ) and Keiletus moorei ( Cheyletus moorei );

- Nematodes (Nematodes): byeoip nematode (Aphelenchoides besseyi ) and strawberry subtle ( Nothotylenchusacris ), etc.

One example of a method for controlling pests using the composition of the present application is to spray the composition of the present application on a pest or pest habitat of a treatment target. "Pest habitat" means the area where the pest actually lives, as well as at least the area where the pest passes or moves. In addition, it means an area in which a habitat or passage of a pest is assumed, as well as an area in which the pest is preferably eliminated.

Examples of plants to which the compositions or methods herein can be applied are as follows:

-Crops: corn, rice, wheat, barley, rye, oats, sorghum, cotton, soybean, peanut, buckwheat, sugar beet, rapeseed, sunflower, sugar cane and tobacco;

-Vegetables: Eggplant and vegetables (eggplant, tomato, bell pepper, pepper, potato, etc.), gourd and vegetables (cucumber, pumpkin, zucchini, watermelon, melon, etc.), cruciferous vegetables (wagered radish, turnip, horseradish, kohlrabi, cabbage, etc.) Cabbage, black mustard, broccoli, cauliflower, rapeseed, etc.), asteraceae vegetables (burdock, garland chrysanthemum, artichoke, lettuce, etc.), lilies and vegetables (green onion, onion, garlic, asparagus, etc.), parsley (carrot, parsley, celery, etc.) , Parsnip, etc.), pollock vegetables (spinach, chard, etc.), lamiaceae vegetables (perilla, mint, basil, etc.), strawberries, sweet potatoes, yams, taro, etc.;

-Fruit trees: causal fruits (apple, pear, Japanese pear, Chinese quince, quince, etc.), stone fruits (peach, plum, nectarine, loquat, cherry, apricot, prune, etc.), citrus fruits (Wenzhou mandarin, orange, lemon, lime, etc.) Grapefruit), nuts (chestnuts, walnuts, hazelnuts, almonds, pistachios, cashews, macadamia nuts, etc.), berries (blueberries, cranberries, blackberries, raspberries, etc.), grapes, persimmons, olives, loquats, bananas, coffee, dates , Coconut, oil palm, etc.;

-Trees other than fruit trees: green tea, mulberry, flower tree (azalea, camellia, hydrangea, baby camellia, Japanese star anise, cherry tree, tulip tree, lagerstroemia tree, gypsy tree, etc.), street trees (ash, birch Tree, Dogwood, Eucalyptus, Ginkgo, Lilac, Maple, Oak, Poplar, Bacterial Tree, Taiwan Style Tree, Plane Tree, Zelkova, Japanese Cypress, Fir, Hem-Fir, Juniper, Pine, Spruce, Yew Tree , spruce, elm, Aesculus turbinata, etc.), sanhosu (coral tree), or Hansong (podocarpus), cedar, cypress, croton (croton), Euonymus japonicus (Euonymusjaponicus), Photinia glabra (Photiniaglabra), etc .;

-Lawn : Grass (zoysiagrass, Zoysiamatrella , etc.), umbrella grass ( Cynodondactylon, etc.), top (Bent grass) ( Agrostisalba ), creeping bent grass, highland bent grass, hiland bent), bluegrass (meadow grass, bird grass, etc.), fescue (tall fescue), chewings fescue, creeping red fescue red fescue, etc.), ryegrass (darnel, ryegrass, etc.), orchard grass, timothy grass, etc.;

-Others: flowers (rose, carnation, chrysanthemum, prairie gentian), gypsophila, gerbera, marigold, salvia, petunia, verbena, tulip , Aster, gentian, lily, pansy, cyclamen, orchid, lily of the valley (convallaria), lavender, stock, ornamental cabbage, primula, poinsettia, gladiolus ), cattleya, daisy, cymbidium, begonia, etc.), biofuel plants (Jatropha, safflower, camelina, switchgrass, silver grass ( Miscanthus), reed canary grass, giant reed, kenaf, cassava, willow, etc.), ornamental plants, etc.

The pest control composition of the present invention may be applied to plants or plant cultivation areas for pest control. Plants as used herein include stems or leaves of plants, flowers of plants, fruits of plants, seeds of plants, and the like. Examples of application methods include application to stems and leaves of plants, such as foliage application; Application to the seeds of plants; And applications to areas where plants are cultivated, such as soil applications and submerged applications.

The composition for controlling pests according to the present invention is very effective by reducing the decrease in potency due to temperature during storage of the composition for controlling pests by excellently improving the stability problem under moisture and temperature conditions, which are a problem in conventional pest control compositions. to be.

1 to 3 are graphs measuring the number of active spore colonies according to time when stored at room temperature and high temperature (54° C.) of the control and pest control composition samples according to an embodiment of the present invention, respectively.

Hereinafter, the present invention will be described in detail through Examples and Experimental Examples, but the following Examples and Experimental Examples are only illustrative of the present invention, and the scope of the present invention is not construed as being limited to the following Examples and Experimental Examples. .

Preparation of ingredients

Pathogenic spore strains include Beauveria bassiana ( Beauveria bassiana ) ERL836 (KCCM11506P) was used. For the cultivation of the fungus, solid culture was performed using grain as a medium material. For this, first, spores of Boberia bassiana ERL836 stored at -70°C were spread on potato agar medium (Potato Dextrose Agar), and then cultured at 25°C for 7 days. Spores were recovered, inoculated into millet, and cultured for 1 week to perform seed culture. For this culture, millet was immersed in water to sufficiently contain moisture, and then put in a steamer and steamed for 10 minutes or more at a high temperature of 100°C or higher. The increased millet was cooled at room temperature, and the seed culture was inoculated and mixed. The mixture was incubated at 25° C. for 5 days or longer, and the culture was dried at low temperature to recover spores. When the number of spores in the original formulation was 1 X 10 ⁹ CFU/g or more, it was taken as normal culture.

As an oily solvent, silicone oil (KF96), mineral oil (8042-47-5, Sigma), paraffin oil (CAS# 64742-55-8), and enspray (SK EnSpray: paraffin oil 98.0~99.0%) and auxiliary agents (polyoxy) Ethylene lauryl ether, glycerol monooleate, oleic acid, etc.) containing 1.0 to 2.0%) was used.

As surfactants, DSS (dioctyl sulfosuccinate sodium, anionic, HLB=11), Dow5200 (Lauryl PEG/PPG-18/18 Methicone, nonionic, HLB=6.8), Dow5600 (Cetyl Diglyceryl Tris(Trimethylsiloxy)silylethyl Dimethicone, nonionic , HLB=2), KF-6017 (PEG-10 dimethicone, HLB=4.5), SPAN80 (Sorbitan monooleate, nonionic, HLB=4.3), SPAN85 (Sorbitan monooleate, nonionic, HLB=1.8), CTAB (Hexadecyl trimethyl ammonium bromide, cationic, HLB=9.8), Triton X45 (Octylphenol Ethoxylate, nonionic, HLB=9.8), Brij93 (Polyoxyethylene (2) oleyl ether, nonionic, HLB=4), Lecithin (CAS# 8002-43- 5, zwitterionic, HLB=4), and PEG400 dioleate (PEG-8 dioleate, nonionic, HLB=8.3) were used.

As a porous material, zeolite 4A microparticles (pore size about 4Å, diameter ~10μm) (ZeoBuilder, Korea), zeolite 4A rod (Zeolite 4A rod; pore size about 4Å, diameter about 1.6mm) (large Jeonghwageum, Korea), molecular sieve X13 (Zeolite 13X; pore size about 10Å, diameter ~2μm) (CAS# 63231-69-6), and S-gel (silica gel; pore size about 60Å, diameter 40-75 μm) ) (Sigma, Supelco) was used.

Experimental Example 1: Analysis of storage stability according to porous materials

Boberia bassiana ERL836, the original pathogenic spore, was uniformly crushed with a mortar. Liquid samples of oily solvent, surfactant, and porous material according to the compositions described in each table were prepared. Specifically, after 1 hour after mixing the oily solvent and the porous material, a surfactant was added and then vortexed, and then sterilized at 80℃ for 30 minutes, and after adding the spore culture dry matter, vortexing was added for 1 minute to prepare a liquid sample. I did. Sample samples were prepared by mixing 10% by weight of the pulverized spore culture dry matter and 100% by weight of each of the liquid samples, and the control group was prepared using only the spore culture dry matter. Each test sample was stored at room temperature (25°C) and 54°C, respectively. Each stored sample was diluted with saline (NaCl 0.85w/v%) for a total of 6 weeks at 1-week intervals, and then spread on PDA (Tartaric acid) medium and cultured in an incubator at 25°C. The number of active spore colonies was counted (unit: CFU (Colony forming unit) / mL) through viable spore count.

Content (% by weight) division menstruum Surfactants Porous material Control - - - - - - Comparative Example 1-1 Distilled water 98.5 DSS 1.5 - - Comparative Example 1-2 Paraffin wax 98.5 DSS 1.5 - - Example 1-1 Paraffin wax 93.5 DSS 1.5 Zeolite 4A microparticles 5 Example 1-2 Paraffin wax 96 DSS 1.5 Zeolite 4A rod 2.5

In the case of the control group (100% spore culture dry matter), spores were maintained until 6 weeks when stored at room temperature, but spores were not observed from week 1 when stored at 54°C (FIG. 1), which indicates that spores are unstable at high temperatures. In the case of Comparative Example 1-1 in which the solvent was mixed with distilled water, all spores died within one week at both room temperature and 54°C, and no spores were observed from week 1, which indicates that spores are very unstable not only at high temperature but also under moisture conditions. . In the case of Comparative Example 1-2, which contained an oily solvent and surfactant, but did not contain any porous material, similar to the control, spores were maintained until week 6 when stored at room temperature, but spores were not observed from week 1 in storage at 54°C. Was not (Fig. 1), which indicates that the spores are unstable at high temperatures.

However, in the case of Example 1-1, which additionally included zeolite microparticles (pore size of about 4Å, diameter ~10μm) as a porous material, the spore concentration was maintained for 6 weeks when stored at room temperature, and spores were maintained for 6 weeks when stored at 54°C. It was observed and maintained above the effective level of the drug, 1.0E + 05 cfu/mL (Fig. 1). Similarly, in the case of Example 1-2 including a zeolite rod-like (pore size of about 4Å, diameter of about 1.6mm) as a porous material, the spore concentration was maintained for 6 weeks at both room temperature and 54°C (FIG. 1). Through these results, it can be confirmed that the presence of the porous material improved the stability of spores against moisture and high temperatures. Accordingly, experiments were conducted using various porous materials to determine which elements of the porous material contributed to the improvement of the stability of spores.

Content (% by weight) division menstruum Surfactants Porous material Comparative Example 2 Silicone oil 93.5 Dow5200 1.5 X13 5 Example 2-1 Silicone oil 93.5 Dow5200 1.5 Zeolite 4A microparticles 5 Example 2-2 Silicone oil 93.5 Dow5200 1.5 Zeolite 4A rod 5

As shown in Table 2, under the conditions of using the same solvent and surfactant, different types of porous materials were tested. Comparative Example 2, including molecular sieve X13 (zeolite 13X; pore size about 10Å, diameter ~2μm) as a porous material, significantly decreased stability to less than 1.0E + 05 cfu/mL, which is a medicinal level from the 3rd week when stored at 54°C. On the other hand, In the case of Example 2-1, including zeolite microparticles (pore size about 4Å, diameter ~10μm) as a porous material, the spore concentration was maintained until the 6th week when stored at room temperature, and the spores were at the effective level until 6 weeks even at 54℃. 1.0E + 05 cfu / mL or more was observed (Fig. 2). Similarly, in the case of Example 2-2 including a zeolite rod (pore size of about 4Å, diameter of about 1.6mm) as a porous material, spores were maintained above the effective level for up to 6 weeks at both room temperature and 54°C storage.

Through these results, it was estimated that among the porous materials, a porous material having a pore size of about 3 to 5 Å improved the stability of spores against moisture and high temperatures. Therefore, experiments were conducted with different types of oily solvents and surfactants for porous materials whose pore size is outside the above range, and whether the type of oily solvent or surfactant could affect the moisture and high temperature stability of the spores. I checked.

Content (% by weight) division menstruum Surfactants Porous material Comparative Example 3-1 Silicone oil 93.5 Dow5600 1.5 X13 5 Comparative Example 3-2 Silicone oil 93.5 KF 6017 1.5 X13 5 Comparative Example 3-3 Mineral oil 93.5 Dow5200 1.5 S-gel (60A) 5 Comparative Example 3-4 Mineral oil 93.5 DSS 1.5 X13 5 Comparative Example 3-5 Mineral oil 93.5 Span 80 1.5 X13 5 Comparative Example 3-6 Mineral oil 93.5 CTAB 1.5 X13 5 Comparative Example 3-7 Mineral oil 93.5 Triton X45 1.5 X13 5 Comparative Example 3-8 Mineral oil 93.5 DSS 1.5 S-gel 5

As shown in Table 3, in a formulation comprising a molecular sieve X13 (zeolite 13X; pore size about 10 Å, diameter ˜2 μm) or S-gel (silica gel; pore size about 60 Å, diameter 40-75 μm) as a porous material, Experiments were conducted with different types of surfactants. As a result, in all of Comparative Examples 3-1 to 3-8, when stored at 54° C., the spore concentration was less than 1.0E + 05 cfu/mL from the second week, and the stability was significantly reduced.

These results indicate that when the pore size of the porous inorganic material is 10 Å or more, the stability of the spores against moisture and high temperatures is not improved even if the type of surfactant is different.

Therefore, from the above results, it was found that a porous material having a pore size in a certain range, specifically less than 10 Å, preferably about 3 to 5 Å, is a decisive factor in improving the stability of spores against moisture and high temperatures. I can.

Experimental Example 2: Storage stability analysis according to surfactant

For a porous material having a pore size of about 3 to 5 Å, an experiment was conducted by different types of surfactants. Specifically, a sample was prepared in the same manner as in Example 1, and the number of active spore colonies was counted (unit: CFU (Colony forming unit)/mL).

Content (% by weight) division menstruum Surfactants Porous material Example 4-1 Enspray 97.5 - - Zeolite 4A rod 2.5 Example 4-2 Enspray 96 Brij93 1.5 Zeolite 4A rod 2.5 Example 4-3 Enspray 96 Span 80 1.5 Zeolite 4A rod 2.5 Example 4-4 Enspray 96 Span 85 1.5 Zeolite 4A rod 2.5 Example 4-5 Enspray 96 DSS 1.5 Zeolite 4A rod 2.5 Example 4-6 Enspray 96 Lecithin 1.5 Zeolite 4A rod 2.5 Example 4-7 Paraffin wax 96 Brij93 1.5 Zeolite 4A rod 2.5 Example 4-8 Paraffin wax 96 Span 80 1.5 Zeolite 4A rod 2.5 Example 4-9 Paraffin wax 96 Span 85 1.5 Zeolite 4A rod 2.5 Example 4-10 Paraffin wax 96 DSS 1.5 Zeolite 4A rod 2.5 Example 4-11 Paraffin wax 96 Lecithin 1.5 Zeolite 4A rod 2.5

As a result, as shown in Examples 4-1 to 4-11, as long as a porous material having a pore size of about 3 to 5 Å is included, spore concentration at both room temperature and 54° C. for 6 weeks regardless of the type of surfactant. Was maintained (Fig. 3).

Taking all the above results together, when the pore size of the porous material is about 3 to 5 Å, the stability of the spores against moisture and high temperatures is improved regardless of the type of the tested surfactant and the shape of the porous material. It can be seen that long-term storage stability is maintained for more than a month.

Claims (17)

Pathogenic fungi or spores thereof,
A porous inorganic material with a pore size of 3 to 5 Å,
Oily solvent, and
Containing a surfactant,
A composition for controlling pests with improved storage stability at 50°C or higher. delete The composition for controlling pests according to claim 1, wherein 1.0% or more of the pathogenic fungi or spores thereof at 50° C. or more maintains survival for one week or more. The method of claim 1, wherein the pathogenic fungi or spores thereof are, Beauveria genus, Isaria genus, Lecanicillium genus, Nomuraea genus, Celletotrichum ) genus Fusarium (Fusarium) in blood Saratov Tora (Phytophthora), A's Klee Loti California (Sclerotinia), Metairie Clear (Metarhizium), A par indeed My process (Paecilomyces) in, in tying yarn Solarium (Verticillium), Trichoderma (Trichoderma), A heosyu telra (Hirsutella) genus Aspergillus (Aspergillus), An ASCII Sonia (Aschersonia) into, or Metarhizium (Metarrhizium ) fungi or spores thereof, a composition for controlling pests. The composition for controlling pests according to claim 1, wherein the pathogenic fungi or spores thereof are Boberia bassiana or spores thereof. The method of claim 1, wherein the pathogenic fungi or spores thereof are contained in an amount of 0.1 to 90% by weight, based on 100% by weight of the composition for controlling pests. The composition for controlling pests according to claim 1, wherein the porous inorganic material is zeolite. The composition for controlling pests according to claim 1, wherein the porous inorganic material is contained in an amount of 0.1 to 90% by weight based on 100% by weight of the composition for controlling pests. The composition for controlling pests according to claim 1, wherein the surfactant is a nonionic, cationic, anionic or amphoteric surfactant. The composition for controlling pests according to claim 1, wherein the surfactant is a nonionic, cationic, anionic or amphoteric surfactant having an HLB value of 1 to 17. The composition for controlling pests according to claim 1, wherein the surfactant is a nonionic, cationic, anionic or amphoteric surfactant having an HLB value of 1 to 7, or 10 to 17. The method of claim 1, wherein the surfactant is DSS (dioctyl sulfosuccinate sodium), lauryl PEG/PPG-18/18 methicone, cetyl diglyceryl tris (trimethylsiloxy) silylethyl dimethicone, PEG-10 dimethicone, Sorbitan monooleate, hexadecyl trimethyl ammonium bromide, octylphenol ethoxylate, polyoxyethylene-2-oleyl ether, lecithin, and at least one selected from the group consisting of PEG400 dioleate, pest control composition. The composition for controlling pests according to claim 1, wherein the surfactant is contained in an amount of 0.1 to 90% by weight based on 100% by weight of the composition for controlling pests. The composition for controlling pests according to claim 1, wherein the oily solvent is at least one selected from the group consisting of mineral oil, paraffin oil, silicone oil, vegetable oil, fish oil and animal fatty oil. The composition for controlling pests according to claim 1, which is in the form of a concentrate for spraying. Claims 1 and 3 to 15, comprising the step of applying the composition for controlling any one of the pest control of the pest, the habitat of the pest, or a crop susceptible to pests, pest control method. Pathogenic fungi or spores thereof,
A porous inorganic material with a pore size of 3 to 5 Å,
Oily solvent, and
A method for stabilizing the composition for controlling pests of any one of claims 1 and 3 to 15, comprising the step of mixing and storing a surfactant.

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