KR102167537B1 - The composition for preventing patella luxation and muscle strengthening for companion animals - Google Patents

Abstract

The present invention relates to a composition for preventing patella dislocation and strengthening muscles for companion animals.
The composition for preventing patella dislocation and strengthening muscles for companion animals according to the present invention includes whey protein isolate powder and boswellia gum resin extract.
According to the above configuration, the present invention includes Boswellia gum resin extract and whey protein isolate powder as active ingredients, and is prepared in a tablet type, so that it can be easily ingested and prevents inflammation due to patella dislocation of companion animals and strengthens muscles. I can.

Description

Composition for preventing patella dislocation and strengthening muscles for companion animals {THE COMPOSITION FOR PREVENTING PATELLA LUXATION AND MUSCLE STRENGTHENING FOR COMPANION ANIMALS}

The present invention relates to a composition for preventing patella dislocation and strengthening muscles for companion animals, and in more detail, by preparing a tablet type containing boswellia gum resin extract and whey protein isolate powder as active ingredients, it can be conveniently ingested. The present invention relates to a composition for preventing the patella dislocation and strengthening muscles for companion animals that can strengthen muscles and prevent inflammation caused by patella dislocation of companion animals.

As the world's aging society and fertility rate decline, the number of households with companion animals and 10 million people in Korea has entered the era, and the average life span of dogs and cats, representative companion animals that was only 10 years ago, is now about 15-20. As the years increase, the demand for systematic nutrition for improving the health of elderly animals is also increasing rapidly.

On the other hand, among various diseases of companion animals, especially in the case of patella dislocation, which occurs frequently in small dogs loved by people because it is easy to raise indoors such as maltese, poodle, pomeranian, yorkshire terrier, chihuahua, etc. It is a disease that occurs due to the deviation of the body and occurs mainly as a congenital disease, and it occurs as an acquired disease caused by external shock.

As described above, when the patella is dislocated, the ligaments and muscles attached to the patella grow abnormally and affect the walking posture, so many people who have dogs have a tendency to pay great attention to the prevention and management of patella dislocation.

These patellas are located in front of the femoral pulley to smooth the movement of the knee, and play a role of connecting the muscles in front of the thigh and the tibia.If the patella is out of the pulley, it leads to pain, inflammation, swelling, and activity disorders. Primary diseases can occur, and this is mentioned as an important management institution for companion animals.

In order to improve this, surgical correction is mainly recommended for the treatment of patella dislocation as a conventional treatment method, but it is necessary to fundamentally improve the conventional treatment method accompanied by rehabilitation because of high cost burden, side effects from surgery, and high possibility of recurrence. to be.

Korean Patent Publication No. 10-2016-0130615 (published on November 14, 2016) Korean Patent Publication No. 10-2004-0073449 (published on August 19, 2004) Korean Patent Publication No. 10-2018-0036167 (published on April 09, 2018)

The present invention includes Boswellia gum resin extract and whey protein isolate powder as active ingredients, and is prepared in a tablet type, so that it can be easily ingested, prevents inflammation caused by dislocation of the patella of the companion animal, and can strengthen muscles. It is to provide a composition for preventing patella dislocation and strengthening muscles.

In addition, the present invention separates Boswellia serrata extract having a high effect of synthesizing muscle protein and protects chondrogenic cells and inhibits cartilage-destructive substances, protects cartilage by increasing cartilage components, and inhibits arthritis through inhibition of inflammatory substances. It is to provide a composition for preventing patella dislocation and strengthening muscles for companion animals containing whey protein.

In addition, the present invention prevents damage such as joint inflammation due to dislocation of the patella by strengthening cartilage and muscle tissue around the knee, which actually plays a role in maintaining health and function of the joint as well as bones and joints, and improves the health and function of the knee joint. It is intended to provide a composition for preventing patella dislocation and strengthening muscles for companion animals that can be manufactured in a tablet type so that it can be easily and conveniently consumed without side effects.

Various problems to be solved by the present invention are not limited to the problems mentioned above, and other problems that are not mentioned will be clearly understood by those skilled in the art from the following description.

The composition for preventing patella dislocation and strengthening muscles for companion animals according to the present invention includes whey protein isolate powder and boswellia gum resin extract.

In addition to the whey protein isolate powder and boswellia gum resin extract, chicken, tapioca starch, potato starch, pea flour, cheese, chicken oil, fructo-oligosaccharide, vitamin C, and glycerin may be further included.

The chicken 25 to 30 parts by weight, tapioca starch 12 to 18 parts by weight, potato starch 20 to 25 parts by weight, pea flour 5 to 10 parts by weight, whey protein isolate powder 3 to 7 parts by weight, Boswellia gum resin extract 3 to It may be included in a weight ratio of 7 parts by weight, 0.5 to 3 parts by weight of cheese, 2 to 6 parts by weight of based oil, 2 to 6 parts by weight of fructo-oligosaccharide, 0.1 to 1.5 parts by weight of vitamin C, and 1 to 5 parts by weight of glycerin.

The chicken, tapioca starch, potato starch, pea powder, whey protein isolate powder, Boswellia serrata extract, cheese, chicken oil, fructo-oligosaccharide, vitamin C, and glycerin are mixed and then prepared in a tablet type.

In addition to the Boswellia gum resin extract, further comprising a Boswellia serrata extract, wherein the Boswellia serrata extract is included in a weight ratio of 50 to 100 parts by weight based on 100 parts by weight of the Boswellia gum resin extract, The Boswellia serrata extract is prepared by washing with purified water after preparing the Boswellia tree to remove foreign substances, and the washed Boswellia tree in a shade maintained at a temperature of 23 to 27°C and a humidity of 55 to 65%. The first dried boswellia tree for 10 to 10 hours, the first dried boswellia tree cut into length units of 1 to 3cm, the minced boswellia tree was heated and steamed with water vapor for steaming, but the chopped boswellia tree The steaming of the lia tree consists of a first steaming step and a second steaming step, and the first steaming step is a step of heating the chopped Boswellia tree with water vapor at a temperature of 100 to 110°C for 5 to 15 minutes, The second steaming step is a step of heating the heated Boswellia tree with water vapor at a temperature of 120 to 130°C for 20 to 40 minutes, and the steamed Boswellia tree at a temperature of 60 to 70°C from 100 to 300 After the second drying for a minute, the second dried Boswellia tree is sprayed with a medium composition, minerals, and Bacillus culture solution and fermented to produce a fermented Boswellia tree, but the fermentation of the Boswellia tree 2 After adding the medium composition, minerals, and Bacillus bacteria culture to the dried Boswellia tree and maintaining a temperature of 60 to 70°C and a humidity of 50 to 55%, fermentation for 3 to 6 days, and the second dried boss Sprayed at a weight ratio of 10 to 30 parts by weight of a medium composition, 5 to 10 parts by weight of minerals and 1 to 5 parts by weight of Bacillus culture solution based on 100 parts by weight of Wellia trees, and the medium composition is peptone, sodium citrate, and Consisting of potassium dibasic (Potassium phosphate dibasic) and distilled water, the peptone, sodium citrate (Sodium c itrate), potassium phosphate dibasic, and distilled water, respectively, in a weight ratio of 5 to 10 parts by weight of peptone, 0.3 to 0.5 parts by weight of sodium citrate, 0.2 to 0.4 parts by weight of potassium dibasic, and 60 to 80 parts by weight of distilled water. It is made by mixing, and the Bacillus Bacillus culture solution is produced by mixing Bacillus Bacillus and water, but the Bacillus Bacillus mix 10 to 15% by weight of brown sugar in godubap, put rice husks on the prepared medium and cover with a cloth, at a temperature of 22 to 24°C. Cultured for one week while spraying water twice a day to produce Bacillus bacteria, inoculated again in a medium mixed with rice bran and brown sugar in a 50:50 weight ratio and cultured at 30° C. for 1 week, and the fermented Boswellia serrata extract is prepared by extracting the Boswellia tree, and after the Boswellia serrata extract is introduced into a cryoconcentrator, the temperature of the cryoconcentrator is maintained at 15 to 17°C. , The Boswellia serrata extract is frozen at 5 to 10% by weight of the initial weight, and the acetic acid ice crystal is removed from the mixture of the frozen Boswellia serrata extract and acetic acid ice crystal to obtain a Boswellia serrata extract. Boswellia serrata extract powder prepared by using the Boswellia serrata extract from which the ice crystal has been removed may be used.

Bacillus subtilis, Bacillus subtilis, Bacillus subtilis, Bacillus megaterium, Bacillus licheniformis, and Bacillus pumilus ) Any one or more selected from the group consisting of is used, and the extraction of the fermented Boswellia tree is 85 to (a) 85 to 15 ℓ of an aqueous solution of 70% (v/v) ethanol in 1500 to 2000 g of fermented Boswellia tree. After the first extraction at 90°C for 3-4 hours, the secondary extraction was completed for 2 to 3 hours at 85 to 90°C with 4 to 6ℓ of 70% (v/v) ethanol aqueous solution, and then 1000 to 1500 in a vacuum rotary evaporator. Preparing an extract until it becomes ㎖; And (b) adding ethanol to the extract, diluting it by 2 to 3 times by weight, centrifuging at 14,000 to 15,000 rpm for 20 to 30 minutes to completely remove polymer fibers and solids, and then adding 1.5 to 2.0 times the weight of the collected supernatant. Boswellia serrata extract can be prepared by extracting twice with acetic acid.

Details of other embodiments are included in the detailed description.

The composition for preventing patella dislocation and strengthening muscles for companion animals according to the present invention includes Boswellia gum resin extract and whey protein isolate powder as active ingredients, and is prepared in a tablet type, so that it can be conveniently consumed and dislocated the patella of companion animals. Prevents inflammation caused by and can strengthen muscles.

In addition, the composition for preventing dislocation of the patella and strengthening muscles for companion animals according to the present invention has a function of protecting cartilage cells and inhibiting cartilage-destructive substances, protecting cartilage by increasing cartilage components, and inhibiting joint inflammation through inhibition of inflammatory substances. It contains Boswellia serrata extract and whey protein isolate with high muscle protein synthesis effect.

In addition, the composition for preventing patella dislocation and strengthening muscles for companion animals according to the present invention is not only for bones and joints, but also by strengthening cartilage and muscle tissue around the knee that actually plays a role in maintaining the health and function of the joint. It can be manufactured in a tablet type to prevent damage such as joint inflammation, improve the health and function of the knee joint, and allow easy and convenient consumption without side effects.

It will be fully understood that the embodiments of the technical idea of the present invention can provide various effects not specifically mentioned.

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed content may be thorough and complete, and the spirit of the present invention may be sufficiently conveyed to those skilled in the art.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise.

Unless otherwise defined, all terms including technical or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning of the related technology, and should not be interpreted as an ideal or excessively formal meaning unless explicitly defined in this application. Does not.

Hereinafter, a composition for preventing patella dislocation and strengthening muscles for companion animals according to the present invention will be described in more detail.

The composition for preventing patella dislocation and strengthening muscles for companion animals according to the present invention includes chicken, tapioca starch, potato starch, pea powder, whey protein isolate powder, boswellia gum resin extract, cheese, chicken oil, fructo-oligosaccharide, vitamin C. And glycerin.

In addition, the composition for preventing patella dislocation and strengthening muscles for companion animals according to the present invention includes 25 to 30 parts by weight of chicken, 12 to 18 parts by weight of tapioca starch, 20 to 25 parts by weight of potato starch, 5 to 10 parts by weight of pea flour, Whey protein isolate powder 3 to 7 parts by weight, Boswellia gum resin extract 3 to 7 parts by weight, Cheese 0.5 to 3 parts by weight, Sea oil 2 to 6 parts by weight, Fructo oligosaccharide 2 to 6 parts by weight, Vitamin C 0.1 to 1.5 It is included in a weight ratio of 1 to 5 parts by weight and glycerin.

In the present invention, the chicken is used as a meat protein source, the tapioca starch, potato starch and pea powder are used as a carbohydrate source, and the whey protein isolate powder acts as a high-quality protein source and is effective in strengthening muscles and muscle strength. , The Boswellia gum resin extract can be used to maintain joint and cartilage function and health, and to prevent and improve knee joint inflammation due to patella dislocation.

In addition, the cheese and chicken oil are used to improve palatability, and the fructo-oligosaccharide is used for intestinal health and helps to proliferate beneficial bacteria, inhibit harmful bacteria, absorb calcium and smooth bowel movements, and the vitamin C is an antioxidant, That is, it is used as an antioxidant, and glycerin can be used as a binder for formulation stability when prepared in a tablet type.

In addition, in the present invention, the whey protein isolate powder may be a commercially available whey protein isolate powder, wherein the whey protein isolate powder has a pure protein content of 90% or more because milk fat including lactose and cholesterol are removed. It has the characteristic of high absorption rate in the body, and companion animals (dogs, cats, etc.) subject to ingestion cannot decompose lactose due to a lack of lactose-degrading enzymes, so there is no worry about side effects (digestion, diarrhea, etc.) that may occur when ingesting regular milk. It can be consumed safely.

For example, in the present invention, the whey protein isolate powder contains 90% or more of protein, 0.1% or less of lactose, a protein utilization (PER) of 3.2, and a pure protein utilization of 92. .

In addition, the Boswellia gum resin extract in the present invention is prepared using the resin (gum) of the Boswellia tree, Boswellia is Boswellia serrata ( Boswellia serrata ) Only a very small amount can be collected from the bark of the frankincense tree It is a material recorded in the naturalistic health journal Ayurveda, herbal medicine, Donguibogam, and has been used as a joint and cartilage health material by the National Institutes of Health (NIH) and the Ministry of Food and Drug Safety.

The boswellia was confirmed to protect cartilage and arthritis by protecting cartilage-producing cells, inhibiting cartilage-destructive substances, increasing cartilage components, and inhibiting inflammatory substances in studies in animals. Safety was confirmed, such as persistence of effect and no gastrointestinal disorder, by confirming the decrease in activity disorder and maintaining the improvement effect even after stopping intake.

In the present invention, the boswellia gum resin extract may be a commercially known boswellia gum resin extract, and in the present invention, a Boswellia serrata extract may be further included in addition to the Boswellia gum resin extract. , The Boswellia serrata extract may be included in a weight ratio of 50 to 100 parts by weight based on 100 parts by weight of the Boswellia gum resin extract.

In addition, in the present invention, the Boswellia serrata extract may be used as a Boswellia serrata extract prepared as follows.

In order to prepare the Boswellia serrata extract used in the present invention, first, the Boswellia tree may be prepared and then washed with purified water to remove foreign substances.

Next, the washed Boswellia tree may be first dried in a shaded place with good ventilation in order to prevent the moisture contained in the Boswellia tree from rapidly evaporating during sunlight drying. For example, , The boswellia tree may be first dried for 5 to 10 hours in a shade maintained at a temperature of 23 to 27°C and a humidity of 55 to 65%.

Next, the first dried Boswellia tree may be shredded in units of a certain size, and the first dried Boswellia tree may be shredded so that the active ingredient can be extracted from the Boswellia tree within a short time. In order to be used, it can be pulverized to a length of 1 to 3 cm.

Subsequently, the chopped Boswellia tree can be heated and steamed with water vapor, and the steaming of the chopped Boswellia tree is a first steaming step and a second steaming step in order to effectively steam the chopped Boswellia tree. It can be carried out in two stages, that is, two stages of steaming.

For example, the first steaming step is a step of heating the chopped Boswellia tree with water vapor at a temperature of 100 to 110°C for 5 to 15 minutes, and the second steaming step is a step of heating the heated Boswellia tree It is a step of heating for 20 to 40 minutes with water vapor at a temperature of 120 to 130°C.

Next, the steamed Boswellia tree can be second dried to remove moisture remaining in the Boswellia tree, and the second drying of the Boswellia tree is 100 to 70°C at a temperature of 60 to 70°C. It can be run for 300 minutes.

Thereafter, the second dried Boswellia tree may be fermented by spraying a medium composition, minerals, and Bacillus culture solution and fermenting it to prepare a fermented Boswellia tree. That is, in the fermentation of the Boswellia tree, a medium composition capable of promoting fermentation, minerals, and Bacillus Bacillus culture are added to the second dried Boswellia tree, and a temperature of 60 to 70°C and a humidity of 50 to 55% are maintained. After soaking, it can be fermented for 3 to 6 days, 10 to 30 parts by weight of the medium composition, 5 to 10 parts by weight of minerals, and 1 to 5 parts by weight of the Bacillus bacteria culture solution based on 100 parts by weight of the second dried Boswellia tree. It can be sprayed at a weight ratio.

In the fermentation of the second dried Boswellia tree, the medium composition is composed of peptone, sodium citrate, potassium phosphate dibasic and distilled water, and the peptone, sodium citrate, and agent Potassium dibasic (Potassium phosphate dibasic) and distilled water may be mixed in a weight ratio of 5 to 10 parts by weight of peptone, 0.3 to 0.5 parts by weight of sodium citrate, 0.2 to 0.4 parts by weight of potassium dibasic, and 60 to 80 parts by weight of distilled water, respectively. have.

In the medium composition used in the fermentation of the second dried Boswellia tree, the peptone supplies protein necessary for the growth of microorganisms as a nitrogen source that supplies protein, and the sodium citrate and the second potassium phosphate ( Potassium phosphate dibasic) can supply nutrients with minerals that maintain an optimized state for the growth of microorganisms by adjusting the pH of the medium.

In addition, minerals used in the fermentation of the second dried Boswellia tree are absorbed by the Boswellia tree during fermentation of the Boswellia tree, and are a mineral that is a relatively small amount of nutrients necessary for the growth, maintenance and reproduction of companion animals. It means, and any one or more selected from the group consisting of calcium, phosphorus, potassium, magnesium, manganese, iodine, selenium, molybdenum, and cobalt may be used.

The Bacillus Bacillus culture solution is produced by mixing Bacillus bacillus and water, and the Bacillus Bacillus mix 10 to 15% by weight of brown sugar in godubap, place eco-friendly rice husks on the prepared medium, cover with a cloth, and then at a temperature of 22 to 24° C. 2 a day. Bacillus bacteria are produced by incubating for one week while spraying moisture on the raw growth, and inoculating it again in a medium mixed with rice bran and brown sugar in a 50:50 weight ratio and culturing at 30°C for 1 week to be used as Bacillus bacteria used in the present invention. I can.

In the present invention, the Bacillus subtilis (Bacillus subtilis), Bacillus subtilis spjijepiyi (Bacillus subtilis), Bacillus megaterium (Bacillus megaterium), Bacillus licheniformis (Bacillus licheniformis) and Bacillus fulmis Any one or more selected from the group consisting of (Bacillus pumilus) may be used.

Subsequently, the fermented Boswellia tree can be extracted to prepare a Boswellia serrata extract. Extraction of the fermented Boswellia tree is (a) 70% in 1500-2000 g of fermented Boswellia trees ( v/v) 1st extraction with 10-15ℓ of ethanol aqueous solution at 85-90℃ for 3-4 hours, and then 2nd extraction with 4-6ℓ of 70% (v/v) aqueous ethanol solution at 85-90℃ for 2-3 hours Completing the extraction and preparing an extract until the concentration is 1000 to 1500 ml in a vacuum rotary evaporator; And (b) adding ethanol to the extract, diluting it by 2 to 3 times by weight, centrifuging at 14,000 to 15,000 rpm for 20 to 30 minutes to completely remove polymer fibers and solids, and then adding 1.5 to 2.0 times the weight of the collected supernatant. Boswellia serrata extract can be prepared by extracting twice with acetic acid.

Next, after introducing the Boswellia serrata extract into the cryoconcentrator, the temperature of the cryoconcentrator is lowered to freeze the Boswellia serrata extract, thereby increasing the purity of Boswellia serrata extract and acetic acid ice crystal. Can be formed.

In the freezing of the Boswellia serrata extract, the Boswellia serrata extract contained in the cryoconcentrator may be partially frozen as the temperature of the cryoconcentrator is lowered. Boswellia serrata extract may be formed into large-sized ice crystals by freezing pure acetic acid components around the outer circumferential surface in direct contact with the inside of the freeze-concentrator or crystal nuclei formed in the Boswellia serrata extract.

That is, in the present invention, freezing of the Boswellia serrata extract can concentrate the Boswellia serrata extract using a freeze concentration method, wherein the freeze concentration method is pure at a temperature below freezing point. During the formation of ice crystals, the solute contained in the Boswellia serrata extract, that is, the extract containing the active ingredient of Boswellia serrata, is separated and concentrated into a non-frozen liquid (ie, unfrozen Boswellia serrata extract). In the present invention, the Boswellia serrata extract has a high content of the active ingredient in the Boswellia serrata extract in a short time by concentrating the Boswellia serrata extract using the freeze concentration method as described above, It is possible to increase the concentrated yield of the extract.

The freezing of the Boswellia serrata extract maintains the internal temperature of the cryoconcentrator in which the Boswellia serrata extract is accommodated at a temperature of 15 to 17°C, and the Boswellia serrata extract is 5 to 10 of the initial weight. It can proceed until the weight percent is frozen.

Thereafter, the acetic acid ice crystal may be removed from the mixture of the frozen Boswellia serrata extract and acetic acid ice crystal to separate the Boswellia serrata extract.

In the present invention, the relative concentration of the ice crystal in which the pure acetic acid component was frozen while passing through the Boswellia serrata extract freezing process and the extract (solute; Boswellia serrata) containing the active ingredient of the Boswellia serrata extract It can be separated into an increased Boswellia serrata extract. By removing the acetic acid ice crystal from the frozen Boswellia serrata extract as described above, a frozen Boswellia serrata extract having a higher concentration than in the previous process can be prepared. I can.

Separation of the ice crystal as described above can be performed in various ways depending on the type of the cryo-concentrator, for example, Boswellia serrata in which the concentration is increased by filtering the Boswellia serrata extract in which the acetic acid ice crystals are formed. Obtaining an extract or by scraping the outer peripheral surface of the Boswellia serrata extract on which ice crystals are formed with a scraper to obtain a Boswellia serrata extract having a higher relative concentration of the extract containing the active ingredient of the Boswellia serrata extract. I can.

Subsequently, the Boswellia serrata extract powder from which the ice crystals have been removed may be used to prepare a Boswellia serrata extract powder. For example, the powdering process of the Boswellia serrata extract from which the ice crystals have been removed is frozen. A known powdering process, such as a drying method or a spray drying method, may be used.

Since the process of powdering the Boswellia serrata extract in the present invention is a known technique, a detailed description thereof will be omitted for convenience of description and clarity of the technical idea of the present invention.

Hereinafter, a preferred embodiment of a composition for preventing patella dislocation and strengthening muscles for companion animals according to the present invention will be described in more detail.

<Example>

Chicken 27 parts by weight, tapioca starch 15 parts by weight, potato starch 24 parts by weight, pea flour 8 parts by weight, whey protein isolate powder 5 parts by weight, Boswellia serrata extract 5 parts by weight, cheese 1.5 parts by weight, chicken oil 5 parts by weight , 4 parts by weight of fructo-oligosaccharide, 0.4 parts by weight of vitamin C, and 3 parts by weight of glycerin were included in a weight ratio to prepare a composition for preventing patella dislocation and strengthening muscles for companion animals.

<Comparative Example>

A composition for preventing patella dislocation and strengthening muscles for companion animals was prepared using the same composition as in the Example.In Comparative Example, a composition for preventing patella dislocation and muscle strengthening for companion animals was not included, without Boswellia serrata extract and whey protein isolate powder. A composition for strengthening was prepared.

1. Bone trachea analysis

The composition for preventing patella dislocation and strengthening muscles for companion animals prepared according to the above Examples and Comparative Examples was applied to the bone volume ratio (% bone volume; BV/TV) to the total tissue volume in the region of interest at the distal portion of the patella of the rat, and the number of bone trabeculae. (trabecular number; Tbn), trabecular thickness (TbTh), trabecular separation (TbSp; average distance between trabecular columns) and structure model index (SMI; index of bone trabecular structure) are statistical programs (Microcal OriginPro 80, Originlab, Northampton, MA, USA) was used to analyze, and the analyzed values are shown in Table 1 below.

division Example Comparative example Bone volume percentage (%) 18.77 ± 1.03 13.68 ± 0.33 Number of bone trabeculae (/㎜) 0.32 ± 0.01 0.21 ± 0.03 Thickness of the bone trabecular column (㎜) 0.60 ± 0.03 0.61 ± 0.06 Spacing of bone trabeculae (㎜) 1.60 ± 0.02 2.21 ± 0.18 Structural shape index 2.20 ± 0.64 1.92 ± 0.84

Referring to [Table 1], the composition for preventing patella dislocation and strengthening muscles for companion animals prepared according to the Example showed an increase in bone volume ratio compared to the Comparative Example, and the number of bone trabeculae increased, and the thickness of the trabecular column It can be seen that there was no significant difference.

2. Bone density measurement

Using CTAn software, a composition for preventing patella dislocation and strengthening muscles for companion animals prepared according to Examples and Comparative Examples from the distal part (distal part) end of the tibia to the point where the fibula meets. Was applied as a Region of Interest (ROI) to analyze the microstructure of each bone including the cortical bone, and the bone density was analyzed for the trabecular bone (= bone trabecular bone) excluding the cortical bone. The measured bone mineral density is as shown in Table 2 below.

division Example Comparative example Bone density (mg/cm3) 466.35 ± 29.50 350.44 ± 40.20

Referring to the [Table 2], it was confirmed that the composition for preventing patella dislocation and strengthening muscles prepared for companion animals according to the Examples increased bone density.

In the above, a preferred embodiment of the present invention has been described, but those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. I will be able to. Therefore, it should be understood that the exemplary embodiment described above is illustrative in all respects and is not limiting.

Claims (6)

Chicken 25 to 30 parts by weight, tapioca starch 12 to 18 parts by weight, potato starch 20 to 25 parts by weight, pea flour 5 to 10 parts by weight, whey protein isolate powder 3 to 7 parts by weight, Boswellia gum resin extract 3 to 7 It is included in a weight ratio of 0.5 to 3 parts by weight of cheese, 2 to 6 parts by weight of based oil, 2 to 6 parts by weight of fructo-oligosaccharide, 0.1 to 1.5 parts by weight of vitamin C and 1 to 5 parts by weight of glycerin,
In addition to the Boswellia gum resin extract further includes a Boswellia serrata extract, wherein the Boswellia serrata extract is included in a weight ratio of 50 to 100 parts by weight based on 100 parts by weight of the Boswellia gum resin extract,
The Boswellia serrata extract,
After preparing the Boswellia tree, it is washed with purified water to remove foreign substances, and the washed Boswellia tree is first dried for 5 to 10 hours in a shade maintained at a temperature of 23 to 27°C and a humidity of 55 to 65%, The first dried Boswellia tree is shredded in units of 1 to 3cm in length, and the shredded Boswellia tree is heated and steamed with water vapor for steaming, but the steaming of the shredded Boswellia tree is a first steaming step. And a second steaming step, wherein the first steaming step is a step of heating the chopped Boswellia tree with water vapor at a temperature of 100 to 110°C for 5 to 15 minutes, and the second steaming step is the heated Heating the Boswellia tree with water vapor at a temperature of 120 to 130°C for 20 to 40 minutes, and drying the steamed Boswellia tree at a temperature of 60 to 70°C for 100 to 300 minutes, and 2 A fermented Boswellia tree is prepared by spraying a medium composition, minerals, and Bacillus bacteria culture solution on the dried Boswellia tree, and fermentation of the Boswellia tree is performed on the second dried Boswellia tree. After adding the composition, minerals and Bacillus Bacillus culture medium and maintaining a temperature of 60 to 70°C and a humidity of 50 to 55%, fermentation for 3 to 6 days, and a medium for 100 parts by weight of the second dried Boswellia tree Sprayed at a weight ratio of 10 to 30 parts by weight of the composition, 5 to 10 parts by weight of minerals and 1 to 5 parts by weight of Bacillus culture medium, and the medium composition is peptone, sodium citrate, potassium phosphate dibasic, and Consisting of distilled water, the peptone, sodium citrate, potassium phosphate dibasic, and distilled water are 5 to 10 parts by weight of peptone, 0.3 to 0.5 parts by weight of sodium citrate, 0.2 to 0.4 of potassium diphosphate, respectively. Weight ratio of 60 to 80 parts by weight and distilled water The Bacillus Bacillus culture solution is produced by mixing Bacillus Bacillus and water, and the Bacillus Bacillus mix 10 to 15% by weight of brown sugar in godubap, put rice husks on the prepared medium and cover with a cloth, and then at a temperature of 22 to 24°C. Incubated for 1 week while spraying water on the growth twice a day to produce Bacillus bacteria, inoculated again in a medium mixed with rice bran and brown sugar in a 50:50 weight ratio, and cultured at 30° C. for 1 week, and the fermentation Boswellia serrata extract is prepared by extracting the boswellia tree, and after the Boswellia serrata extract is introduced into a cryoconcentrator, the temperature of the cryoconcentrator is maintained at 15 to 17°C. And, the Boswellia serrata extract is frozen in 5 to 10% by weight of the initial weight, and the acetic acid ice crystal is removed from the mixture of the frozen Boswellia serrata extract and acetic acid ice crystal to extract Boswellia serrata The composition for preventing patella dislocation and strengthening muscles for companion animals, characterized in that using the Boswellia serrata extract powder prepared using the Boswellia serrata extract from which the ice crystal has been removed.
delete delete delete delete The method of claim 1,
Bacillus subtilis, Bacillus subtilis, Bacillus subtilis, Bacillus megaterium, Bacillus licheniformis, Bacillus licheniformis and Bacillus pumilus ) Any one or more selected from the group consisting of is used,
Extraction of the fermented Boswellia tree is (a) primary extraction at 85 to 90°C for 3 to 4 hours with 10 to 15ℓ of 70% (v/v) ethanol aqueous solution to 1500 to 2000 g of fermented Boswellia tree Completing the secondary extraction for 2 to 3 hours at 85 to 90 °C with 4 to 6 ℓ of an aqueous 70% (v/v) ethanol solution, and preparing an extract until it becomes 1000 to 1500 ㎖ in a vacuum rotary evaporator; And (b) adding ethanol to the extract, diluting it by 2 to 3 times by weight, centrifuging at 14,000 to 15,000 rpm for 20 to 30 minutes to completely remove polymer fibers and solids, and then adding 1.5 to 2.0 times the weight of the collected supernatant. A composition for preventing patella dislocation and strengthening muscles for companion animals, characterized in that to prepare Boswellia serrata extract by extracting twice with acetic acid.