KR102132514B1 - A cosmetic composition containing hot water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium - Google Patents

Abstract

The present invention extracts the root (oil) portion of cucumber grass (Sanguisorba officinalis L.) having specific antimicrobial activity of Propionibacterium acnes with hot water, and extracts and ethyl acetate fractions thereof It is a cosmetic composition comprising a.

Description

A cosmetic composition containing hot water extract or its ethyl acetate fraction from Sanguisorba officinalis having specific anti-microbial activity against acne-inducing bacterium}

The present invention extracts the root (oil) part of cucumber (Sanguisorba officinalis L.) having specific antimicrobial activity of propionibacterium acnes with hot water and extracts or ethyl acetate thereof It relates to a cosmetic composition comprising a fraction.

Acne vulgaris is the most common inflammatory disease in the skin's pilosebaceous unit, most commonly on the face of adolescent men and occasionally on the chest, shoulders, etc. The main cause is due to the effect of the male hormone (androgen) which induces excessive sebum production along with the expansion of the sebaceous glands and formation of comedone. The other main causes are due to abnormal detachment of the hair follicle epithelium (skin keratin) and proliferation of propionium bacterium acne.

Propionibacterium acnes are anaerobic, gram positive, and bacillus that live as symbiotic bacteria in the sebaceous gland unit. Abnormal peeling of the hair follicle epithelium creates anaerobic conditions in the hair follicle, thereby promoting the propagation of propionibacterium acnes. In addition, propionibacterium acne secretes lipolytic enzymes and chemotactic factors to metabolize excessively secreted glycerol components of sebum triglyceride into free fatty acids. These extra free fatty acids promote the production of inflammatory white blood cells around the hair follicles. On the other hand, inhibiting the growth of propionibacterium acne can minimize the rupture of cotton and suppress the development of acne.

Various oral or transdermal antibiotics such as tetracycline, erythromycin and clindamycin have been used to treat acne. Long-term use of antibiotics with this broad spectrum can cause overgrowth of Candida albicans and antibiotic resistance. In addition, transdermal chemicals such as benzoyl peroxide, azelaic acid, and retinoic acid, which are known as anti-acne treatments, have a side effect such as skin irritation and contact dermatitis.

In order to solve the above problems, many antibacterial plants have been recently reported internationally. Some of them can be used as a substitute for acne treatment. The majority of extracts showing distinct antimicrobial activity against propionium bacterium acne in extracts obtained from target tissues of a single plant, including plants, currently reported are methanol (Patent Documents 0001, 0002, 0003 and Non-Patent Documents 0001, 0002 , 0003, 0004), ethanol (patent documents 0004, 0005, 0006, 0007, 0008 and non-patent documents 0005, 0006, 0007, 0008, 0009, 0010, 0011), hot water (patent documents 0009, 0010 and non-patent documents 0012 ), phosphate buffer solution (patent document 0011), acetone (non-patent document 0013), other organic solvents (non-patent documents 0014, 0015) and water vapor distillation method (patent documents 0012 and non-patent documents 0016) were used.

The “Halal” market is a market that attracts attention as one of the largest in the international market, and it is also a growing market for Muslims. In the case of cosmetics, ethanol, which is widely used for extracting and concentrating active ingredients from plants, cannot be used at all to satisfy halal standards, and polyhydric alcohols cannot be used for animal-derived ingredients.

In addition, strains used in the fermentation process should also be selected and used according to demanding standards, and ethanol produced during the fermentation process may also be a barrier. Therefore, compared with other extraction methods, the extraction method using hot water and hot water, which has the advantage of easily extracting the active ingredient, is the only alternative.

Cucumber (S. officinalis L.), a perennial plant belonging to the family Rosacease, is widely distributed in the Far East, including in Korea. The root of cucumber grass is used in herbal medicine as a herbal medicine (地楡) (non-patent document 0017).

Oil has been mainly used in the treatment of hemostasis and wounds in oriental medicine, and is known to be used externally for dermatitis, mucositis, eczema, and burns (non-patent document 0018). Oil contains ziguglycoside I, II, and pomolic acid. Branches contain quercetin and kaempferol glycosides, and triterpenoid-based saponins such as ursolic acid. Vitamin C on leaves, and chrysanthemin and cyanin on flowers. (Non-Patent Document 0019).

Studies on immunosuppressive activity, antimutagenic effect, nerve cell damage suppression effect, antioxidant effect, prevention of hypersensitivity allergy, anti-cancer, antibacterial effect, and hemostatic action have been reported from cucumber grass based on the above pharmacological components (non-patent document 0017) , 0020, 0021, 0022; 0023, 0024, 0025, 0026, 0027).

As a patent for cucumber extract, Streptococcus mutans-inhibiting oral cleanser composition causing cavities (Patent Document 0013), antiviral active composition against VHSV and IHNV causing sepsis (Patent Document 0014), and skin care effect cosmetic composition (Patent Document) 0015), a composition for preventing and improving diabetes (Patent Document 0016), a composition for preventing and improving Listeri or Salmonella-induced sepsis (Patent Document 0017).

Republic of Korea Patent Publication No. 10-2015-0000723, 2015.01.05. open Republic of Korea Registered Patent Publication No. 10-1507889, 2015.04.07. Announcement Republic of Korea Patent Publication No. 10-2008-0044948, 2008.05.22. open Republic of Korea Patent Publication No. 2002-0003313, 2002.01.12. open Republic of Korea Patent Publication No. 2000-0019981, 2000.04.15. open Republic of Korea Patent Publication No. 10-2012-0086123, 2012.08.22. open Republic of Korea Patent Publication No. 10-2014-00992233, 2014.08.11. open Republic of Korea Patent Publication No. 10-2004-0081533, 2004.09.22. open Republic of Korea Patent Publication No. 10-0700911, 2007.03.28. Announcement Republic of Korea Registered Patent Publication No. 10-0700912, 2007.03.28. Announcement Republic of Korea Patent Publication No. 10-2007-0110589, 2007.11.20. open Republic of Korea Patent Publication No. 2003-0016818, 2003.03.03. open Republic of Korea Patent Publication No. 10-2013-0113018, 2013.10.15. open Republic of Korea Patent Publication No. 10-2016-0150177, 2016.12.29. open Republic of Korea Patent Publication No. 10-2009-0117199, 2009.11.12. open Republic of Korea Patent Publication No. 10-2016-0102638, 2016.08.31. open Republic of Korea Patent Publication No. 10-2017-0055104, 2017.05.19. open

Muddathir et al., J. Woos Sci. 2013, Vol. 59, pp. 426-431 Kim et al., J. Microbial. Biotechnol. 2010, Vol. 20, pp. 82-87 Lim et al., J. Microbiol. 2007, Vol. 45, pp. 473-477 Tsai et al., Food Chem. 2010, Vol. 119, pp. 964-968 Tangje Arung et al., Biodiversitas 2017, Vol. 18, pp. 321-325 Pothitirat et al., Pharm. Biol. 2010, Vol. 48, pp. 182-186 Sharma and Lall, S. Afr. J. Sci. 2014, Vol. 110, Aer. # 2013-0293 Sharma et al., Phytother. Res. 2011, Vol. 25, pp. 517-521 Chomnawang et al., Fitoterapia 2007, Vol. 78, pp. 401-408 Wang et al., J. Agric. Food Chem. 2013, Vol. 61, pp. 12274-12282 Kumar et al., Trop. J. Pharm. Res. 2007, Vol. 6, pp. 717-723 Sathishkumar et al., J. Photocheml. Photo Biol., B: Biology 2010, Vol. 20, pp. 82-87 Vijayalakshimi et al., Int. J. PharmTech Res. 2011, Vol. 3, pp. 320-327 Shah et al., J. Med. Plants Res. 2015, Vol. 9, pp. 755-763 Pothitirat et al., Med. Princ. Pracy. 2010, Vol. 19, pp. 281-286 Qidwai et al., Int. J. Pharm. Sci. Res. 2016, Vol. 7, pp. 2917-2924 An et al., Kor. J. Kor. Soc. Appl. Biol. Chem. 2004, Vol. 47, pp. 244-250 Son et al., J. Kor. Acad. Oral Health 2015, Vol. 28, pp. 97-104 Lee et al., J. Korean Soc. Food Sci. Nutr. 2005, Vol. 34, pp. 1545-1552 An et al., Kor. J. Kor. Soc. Appl. Biol. Chem. 2004, Vol. 47, pp. 131-145 Ban et al., Kor. J. Med. Crop Sci. 2005, Vol. 13, pp. 219-226 Goun et al., J. Ethnopharm. 2002, Vol. 81, pp. 337-342 Hwang et al., J. Physiol. Pathol. Kor. Med. 2014, Vol. 28, pp. 499-505 Kim et al., Nat. Prod. Sci. 2002, Vol. 8, pp. 177-182 Kim et al., Kor. J. Food Cook. Sci. 2011, Vol. 27, pp. 15-26 Park et al., Kor. J. Herb. 2012, Vol. 27, pp. 23-29 Park et al., Korean J. Food Preserv. 2012, Vol. 19, pp 744-750

It is an object of the present invention to provide a cosmetic composition comprising a hydrothermal extract of oil (root of cucumber) or a ethyl acetate fraction thereof having specific antimicrobial activity of Propionibacterium acnes.

In order to achieve the above object, the present invention is to prevent or improve acne containing propionibacterium acnes (Propionibacterium acnes) specific antibacterial activity oil (root of cucumber) hot water extract or its ethyl acetate fraction. Provide a cosmetic composition for.

As described above, according to the present invention, the hydrothermal extract of oil or a fraction thereof has antibacterial activity specific to Propionibacterium acnes, and thus is effective in preventing or improving acne. Therefore, it can be commercialized as a cosmetic composition, more specifically, a cosmetic composition, a soap, and a detergent composition.

Figure 1 is a flow diagram of the active fraction separation process from the hot water extract (HWE) of the oil.
FIG. 2 is a paper disc agar diffusion method that analyzes the antibacterial activity of Propylonibacterium acnes of the oily hot water extract (HWE) and the ethyl acetate fraction (HWE-EA) of the hot water extract. Figure (A) is a photograph of the HWE 10 mg/disk treatment tool (1), no treatment tool (2), and distilled water treatment tool (3) for the inhibition of acne bacteria. Figure (B) is a picture of observing the inhibitory ring against acne bacteria in distilled water treatment (1) and HWE 1 mg/disk treatment (2), 3 mg/disk treatment (3), and 5 mg/disk treatment (4) . Figure (C) observed distilled water treatment (1) and HWE-EA 1 mg/disk treatment (2), 3 mg/disk treatment (3), and 5 mg/disk treatment (4) for the inhibition of acne bacteria It is a picture.
FIG. 3 is a paper disc agar diffusion method of n-hexane, ethyl acetate, n-butanol, and water fractions of hot water extract (HWE) of oil. It is a result of comparing the suppression of acne bacteria.
Figure 4 is a result of confirming the presence or absence of colony formation in a solid medium to determine the minimum inhibitory concentration (MIC) and the minimum sterilization concentration (MBC) for the acne (Propionibacterium acnes) of the hot water extract (HWE) of oil.
Figure 5 is the ethyl acetate (ethyl acetate) fraction of the hot water extract (HWE) of fat oil on a silica gel 60 glass plate and mixed solvent ethyl acetate, n-hexane, chloroform, acetic acid (40:30:30:2, v /v/v/v) and analyzed by UV wavelength 254 nm (A), 366 nm (B) and sulfuric acid spray coloration (C).
Figure 6 is obtained by developing the ethyl acetate (ethyl acetate) fraction of the hot water extract (HWE) of the oil in TLC and analyzed by HPLC. (1) mixed compounds that are not isolated, (2) caffeic acid, (3) ferulic acid, (4) quercetin, and (5) kaempferol.

One embodiment of the present invention can provide a cosmetic composition for improving acne comprising a hot water extract or a fraction thereof of cucumber (Sanguisorba officinalis).

Cucumber is a perennial plant belonging to the family Rosaceae. It is distributed in Korea, Japan, Europe, and Manchuria. When the leaves are picked and poured, they smell like cucumber and are called cucumber grass. In oriental medicine, the root of cucumber grass is used as a medicine called Jiyu. Cucumber root contains 17% tannin, 2.5% saponin, and glycosides called Sanguisorbigenin, and stems and leaves contain triterpene-like substances such as qurcetin, Kaemferol glycosides, and Ursolic acid. Jiyu (root of cucumber grass) is known to have the effect of lowering body heat and stopping bleeding in oriental medicine, and has a detoxifying effect. Oil (root of cucumber) is effective for burn treatment, hemostasis, vomiting suppression, and antibacterial action.

The cosmetic composition of the present invention is not particularly limited, but may be used for external application to the skin or may be taken orally. The cosmetic composition of the present invention contains a base oil extract as an active ingredient and is a basic cosmetic composition (face wash, cream, essence, cleansing foam and cleansing water-like cleansing agent, pack, bodioyl), and color cosmetic composition (Foundation, lipstick, mascara, makeup base), hair product composition (shampoo, conditioner, hair conditioner, hair gel) and soap.

The excipients include, but are not limited to, for example, skin emollients, skin penetration enhancers, colorants, fragrances, emulsifiers, thickening agents and solvents. In addition, fragrances, pigments, disinfectants, antioxidants, preservatives and moisturizers may be further included, and for the purpose of improving physical properties, may include thickeners, inorganic salts, synthetic polymer materials, and the like. For example, in the case of preparing the cleanser and soap with the cosmetic composition of the present invention, it can be easily prepared by adding the above oil extract to a conventional cleanser and soap base. In the case of manufacturing the cream, it can be prepared by adding an oil-based extract or a salt thereof to a cream base of a general oil-in-water type (O/W). Synthetic or natural materials such as proteins, minerals, vitamins, etc. for the purpose of improving physical properties may be additionally added to fragrances, chelating agents, pigments, antioxidants, preservatives, and the like.

The content of the oil extract contained in the cosmetic composition of the present invention is not limited to this, but is preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight relative to the total weight of the total composition. If the content is less than 0.001% by weight, the desired effect of improving skin whitening or pigmentation cannot be expected, and if it is more than 10% by weight, there may be difficulties in safety or formulation.

The hot water extract of the cucumber pool can be extracted from 40°C to 160°C for 1 hour to 5 hours, preferably from 60°C to 120°C for 1 hour to 3 hours, most preferably from 80°C to 120°C Can be extracted for 2 hours.

In the present invention, the cosmetic composition may be characterized by inhibiting the activity of the acne bacteria Propionibacterium acnes (Propionibacterium acnes).

In the present invention, the hot water extract of the cucumber (Sanguisorba officinalis) may be characterized in that it is one or more hot water extracts selected from the group comprising flowers, leaves, stems, fruits, seeds, roots and outposts of the cucumber grass, preferably Can be the root of cucumber grass.

In the present invention, the cosmetic composition comprises a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray It may be characterized by having one or more formulations selected from the group, preferably soap or surfactant-containing cleansing.

In the present invention, the hot water extract of the cucumber (Sanguisorba officinalis) may be characterized in that it is included in a concentration of 350 ㎍ / mL to 850 ㎍ / mL, preferably 625㎍ / mL.

In the present invention, the fraction may be characterized in that it comprises an ethyl acetate fraction obtained by fractionation with ethyl acetate from a hot water extract of cucumber (Sanguisorba officinalis).

In the present invention, the ethyl acetate fraction may be characterized in that it contains any one or more active ingredients selected from the group consisting of hydroxycinnamic acids and flavonols.

In the present invention, the ethyl acetate fraction may be characterized as comprising 100 μg/mL to 200 μg/mL concentration, preferably 156 μg/mL.

It is also possible to provide quasi-drugs containing a hot water extract of Sanguisorba officinalis or a fraction thereof.

When the composition of the present invention is used as a quasi-drug additive, the extract or fraction may be added as it is or used with other quasi-drugs or quasi-drug components, and may be suitably used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of use. The quasi-drug composition of the present invention is not limited thereto, but preferably, disinfecting cleaners, shower foams, greens, wipes, detergent soaps, hand washes, masks, ointments, It can be a patch, or a filter filler.

Hereinafter, the present invention will be described in more detail according to examples, but the present invention is not limited by the following examples.

Hot water extract and fractions of oil

1. Manufacture of hot water extract (HWE) of oil

Cucumber roots (oil) were washed with distilled water, dried and then finely crushed with a grinder. The pulverized sample (50 g) was added with 10-fold weight of 100° C., extracted for 2 hours, filtered with Whatman No. 2, and the filtrate was concentrated under reduced pressure at 65±2° C. and frozen in a cryogenic freezer. Then, freeze-drying with a freeze dryer gave 0.4673 g of the final extract. Table 1 shows the yield of hot water extracted from the base oil building.

Yield through extraction of hot water from oil Jiyu building Freeze-dried yield of hot water extract (HWE) 50 g 0.4673 g

2. Preparation of HWE fraction

After adding a certain amount of water to the hot water extract, each fraction was extracted by sequentially mixing n-hexane, ethyl acetate, butanol, and water, which are different polarity extraction solvents, using a separating filter. First, n-hexane was added to distilled water in the same amount as distilled water, mixed well, and left to be divided into two layers, an aqueous layer and an extraction solvent. The extraction solvent was separated, and the remaining filtrate was extracted with ethyl acetate and butanol in turn in the same manner. At this time, the extraction solvent was added in the same amount as the remaining filtrate. The schematic diagram of the extraction and fractionation process is shown in [Figure 1]. As a result of the experiment, the sequential fraction was suspended by distilling 50 g of hot water extract of fat oil into 500 mL of distilled water, and then sequentially fractionating it with n-hexane (500 mL), ethyl acetate (500 mL), n-butanol (500 mL) and distilled water. Got

Antibacterial activity test of plant extracts and fractions

To measure the antimicrobial activity against Gram-negative bacteria (2 species) and Gram-positive bacteria (3 species), the paper disk diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were used. Was evaluated.

1. Target strain and culture conditions

The Gram-positive bacteria used in the present invention, Propionibacterium acne (KCTC3314; Biological Resource Center) was cultured for more than 2 days in anaerobic conditions in brain-heart infusion (BHI) medium, Listeria monocytogenes and Staphylococccus aureus was cultured in BHI medium under aerobic conditions. The Gram-negative bacteria Escherichia coli and Vibrio parahaemolyticus used in the present invention were cultured in liquid in Luria-Bertani (LB) medium. Each inoculated strain is shaken and cultured for 1~2 days at 37°C and 200 rpm for shaking, and the growth and lysis of each bacterium is measured using a Biochrom Libra S22 spectrophotometer. Measured at 600 nm.

2. Paper disc agar diffusion method

The cultured strain was uniformly plated on a BHI agar plate or an LB agar plate using a sterile cotton swab that was adjusted to a constant concentration (absorption value 0.5 at an optical density of 600 nm). After drying the plate, a paper disc (Φ 6 mm, Adventec. USA) was placed on the surface of the plate inoculated with the strain, and each of the hot water extracts was absorbed by 1, 3, 5 or 10 mg/disk. After the sample-treated plate was incubated at 37°C for 48 hours, the antibacterial activity was measured by the size of the inhibition zone around the disc. At this time, four antibiotics (ampicillin, kanamycin, spectinomycin, timentin) were used as controls, and the treatment concentration was 20 ug/disc. As a result of the experiment, strong anti-acne activity was confirmed in the hot water extract 10mg/disk treatment of fat milk [Fig. 2A and Table 2]. Excellent anti-acne activity was also confirmed up to 1 mg/disk treatment in each treatment group by concentration [Fig. 2B]. However, no antibacterial activity was observed for the other 4 bacteria. Through these results, the hot water extract of the oil was found to have specific antibacterial activity against propionium bacterium acne, but no antibacterial activity against other bacteria.

Antibacterial activity against Gram-positive bacteria Propionibacterium acne Sample (10 mg/disc) Growth inhibition ring size (cm) Hot Water Extract (HWE) 3.4 x 3.5 ampicillin ― kanamycin 3.1 x 3.0 spectinomycin ― timentin ―

In addition, excellent anti-acne activity was observed in the ethyl acetate fraction obtained from the hot water extract of fat oil, but was weak or not observed in the hexane, butanol and distilled water fractions [FIG. 3 ]. As a result of treatment by concentration of ethyl acetate fraction, excellent anti-acne activity was confirmed up to 1 mg/disk treatment [Figure 2C].

3. Minimum inhibitory concentration (MIC) and minimum sterilization concentration (MBC) of oil-based hot water extract (HWE) and ethylacetate fraction (HWE-EA) for acne bacteria

Propionibacterium acne was incubated at 37° C. for 24 hours in a test tube containing BHI broth medium. Here, the hot water extract and ethylacetate fraction were diluted in a two-fold dilution method and added to a test tube. After culturing for 48 hours in a test tube inoculated with the bacterial dilution solution and the sample, the minimum concentration that inhibited the visible growth of the bacteria was determined as the MIC. As shown in Table 3 below, the MIC values of the hot water extract and ethyl acetate fraction for Propionibacterium acne were measured to be 625 μg/mL and 156 μg/mL. 100 microliters of the culture medium of the test tube corresponding to a concentration higher than the concentration selected by the MIC was smeared on a solid medium, and the bacteria were cultured, and the number of colonies observed on the plate was directly counted. At this time, the concentration of the extract showing the effect of killing 100% of the number of colonies confirmed was determined by MBC and determined as shown in Tables 3 and 4 below. As shown in Table 3 below, the MBC values of the hot water extract and ethyl acetate fraction for Propionibacterium acne were measured to be 1250 μg/mL and 312 μg/mL.

Minimum inhibitory and minimum sterilization concentrations for propionibacterium acnes of ethyl acetate fractions of oil-based hot water extracts and extracts extract MIC (µg/mL) MBC (µg/mL) Hot Water Extract (HWE) 625 1250 Ethyl acetate fraction (HWE-EA) 156 312

Component analysis of ethyl acetate fraction with anti-acne activity

1.Thin layer chromatography (TLC)

The ethyl acetate fraction having anti-acne activity was silica gel 60 glass plate (20 x 20 cm, Merck, DarDarmstadt, Germany) using ethyl acetate/hexane/chloroform/acetic acid (40:30:30:2, v /v/v/v) developed as a mixed solvent and combined with ethyl acetate fraction and standard materials (caffeic acid, ferulic acid, quercetin, kaempferol) by UV lamp (254 nm and 366 nm wavelength) and sulfuric acid spray coloration method. Rf values were compared and analyzed. As a result of experimenting with the above method, a band similar to the standard substances caffeic acid, ferulic acid, quercetin, and kaempferol Rf was confirmed.

2. High performance liquid chromatography (HPLC)

The ethyl acetate fraction was dissolved in methanol and filtered (0.45 μm filter) to ZORBAX Eclipse Plus C18 column (4.6×150 mm, 5 μm, Agilent Technologies, Lawrence, KS, HPLC) on HPLC (1260 Infinity Quaternary LCSystem, Agilent Technologies, Waldbronn, Germany). USA). Injection volume is 10 μL, mobile phase is solvent A (2% acetic acid in 30% methanol) and B (2% acetic acid in 50% acetonitrile) at a flow rate of 1 mL/min. /v) from 70 to 70 minutes at a ratio of 0:100 (v/v). The content measurement was obtained from the regression equation of the calibration curve prepared using the samples caffeic acid, ferulic acid, quercetin, and kaempferol. As a result of experimenting with the above method, the retention time with the standard substance was compared in the fractions of blue and white by reverse-phase HPLC method to confirm two hydroxycinnamic acids (caffeic acid, ferulic acid) and two flavonols (quercetin, kaempferol) [ Fig. 6]. Among the ethyl acetate fractions of the oil-based hot water extract, several unknown substances were detected, including caffeic acid, ferulic acid, quercetin, and kaempferol, among which the highest ferulic acid content was detected.

Claims (9)

Cucumber (Sanguisorba officinalis) contains a hot water extract or a fraction thereof,
The hot water extract of the cucumber (Sanguisorba officinalis) is characterized in that it is included in a concentration of 350 ㎍ / mL to 625 ㎍ / mL,
The fraction is characterized in that it is an ethyl acetate fraction obtained by fractionation with ethyl acetate from a hot water extract of cucumber (Sanguisorba officinalis),
The ethyl acetate fraction is characterized in that it comprises a concentration of 100 ㎍ / mL to 200 ㎍ / mL,
A cosmetic composition for improving acne, which suppresses the activity of the acne bacterium Propionibacterium acnes. delete The method according to claim 1, wherein the hot water extract of the cucumber (Sanguisorba officinalis) is one or more hot water extracts selected from the group comprising flowers, leaves, stems, fruits, seeds, roots and outposts of the cucumber grass for acne improvement. Cosmetic composition. The method of claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray A cosmetic composition for improving acne, characterized by having at least one formulation selected from the group. delete delete The cosmetic composition for improving acne according to claim 1, wherein the ethyl acetate fraction contains at least one active ingredient selected from the group consisting of hydroxycinnamic acids and flavonols. delete Cucumber (Sanguisorba officinalis) contains a hot water extract or a fraction thereof,
The hot water extract of the cucumber (Sanguisorba officinalis) is characterized in that it is included in a concentration of 350 ㎍ / mL to 625 ㎍ / mL,
The fraction is characterized in that it is an ethyl acetate fraction obtained by fractionation with ethyl acetate from a hot water extract of cucumber (Sanguisorba officinalis),
The ethyl acetate fraction is characterized in that it comprises a concentration of 100 ㎍ / mL to 200 ㎍ / mL, quasi-drug to inhibit the activity of acne bacteria Propionibacterium acnes (Propionibacterium acnes).

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