KR102127706B1 - Preparation for improving blood circulation comprising mixed oriental medicinal extracts - Google Patents

Abstract

The present invention relates to a formulation for improving blood circulation, including a complex herbal herbal extract, more specifically, extracting each component efficiently using various herbal herbal ingredients in consideration of useful functionality and side effects of each component It relates to a formulation for improving blood circulation in the form of pills, tablets, capsules, injections, pouches, drinks, or beverages prepared by using a composition optimized by considering the taste and the like of a product by mixing at an appropriate mixing ratio. The agent for improving blood circulation according to the present invention may be helpful in the prevention and treatment of diseases including hyperlipidemia, arteriosclerosis, cerebral thrombosis, angina pectoris, as well as improving blood flow, improving vascular sclerosis, improving vascular endothelial function, stroke, and Dementia, cardiac hyperactivity, spasms and pain relief and clear blood make it circulate to peripheral blood vessels, so it can be appropriately used for patients who require it.

Description

Preparation for improving blood circulation comprising mixed oriental medicinal extracts

The present invention relates to a formulation for improving blood circulation, including a complex herbal herbal extract, more specifically, extracting each component efficiently using various herbal herbal ingredients in consideration of useful functionality and side effects of each component It relates to a formulation for improving blood circulation in the form of pills, tablets, capsules, injections, pouches, drinks, or beverages prepared by using a composition optimized by considering the taste and the like of a product by mixing at an appropriate mixing ratio.

Thrombus-related diseases, which account for about 39% of the causes of death worldwide, have been increasing in Korea due to westernization of diet, excessive stress, and aging population.

The global antithrombotic and anticoagulant market is expected to reach $24.4 billion in 2015, and sales are expected to increase gradually by 2022. In particular, increasing incidence, prevalence, and number of diagnoses in developing countries such as China and India will likely contribute to sales growth. It is expected that drugs and treatments that introduce new oral anticoagulants that are superior in safety and effectiveness and that introduce drug delivery methods that will facilitate simple prescription and regulatory compliance will replace the existing treatments.

Examples of thrombolytic enzymes currently in use include streptokinase, eurokinase, and tissue plasminogen activator (t-PA). Plasminogen in vivo is mainly used as plasmin. ) To dissolve thrombus in an indirect way. Plasminogen activators as described above are classified as allergies, fever, local bleeding, short half-life, and expensive medicines, and there is a problem that they are limited to thrombotherapy. Streptokinase and Eurokinase are foreign enzyme substances that convert plasminogen into plasmin, which has side effects such as fever, allergies, local bleeding, and hypotension, and is classified as a very expensive drug treatment drug, so it is limited to therapeutic agents. And has not been used to prevent thrombosis in the body.

Therefore, research on natural plant-derived substances that are safe and have excellent blood circulation improvement efficacy is actively underway, and it is better to develop a formulation that helps improve blood circulation for traditionally proven natural substances rather than synthetic substances that cause side effects. It may be more desirable.

Korean Registered Patent No. 10-1158739 (2012.06.15.) Korean Registered Patent No. 10-1259499 (2013.04.24.) Korean Registered Patent No. 10-1558524 (2015.10.01.)

The present inventors conducted extensive research to develop functional substances targeting natural products based on long-term experience with herbal herbal medicines. As a result, various functional herbal ingredients, as described below, were used to identify useful functionalities and side effects of each component. Efficient extraction of each ingredient in consideration, formulation by appropriate mixing ratio, and formulation prepared using an optimized composition in consideration of the taste of the product has no side effects and is suitable for the treatment of various cardiovascular diseases including improvement of blood circulation By doing so, it has been found that it can be effectively used for patients in need thereof, and has come to complete the present invention.

Accordingly, the object of the present invention is to extract and mix various herbal herbal ingredients efficiently and to prepare them by optimizing the taste, etc. of the product by optimizing or enhancing them, pills, tablets, capsules It is to provide a formulation for improving blood circulation formulated with an agent, an injection, a pouch, a drink, and a beverage.

The object of the present invention as described above is water, natural insects, insects, insects, scorpions, scorpions, 7 kinds of animal herbal medicines, including sediment, mineral minerals and dried radish, including alum, balsamic, county, triceps, rhubarb, Hyperlipidemia, arteriosclerosis, and thrombosis, which are made from 18 kinds of herbal medicines, including Doin, Sprout barley, Noni, Shou, Angelica, Aloe or Musk, Ginseng, Hwanggi, Cornus officinalis, Sansa, Gugamcho, rice water and cerebrum. , Angina pectoris, ischemic stroke and stroke can be achieved by a formulation for improving blood circulation for the prevention or treatment of any one of the diseases.

The above object, in an additional aspect, is prepared by mixing and extracting the whole mixture at a predetermined mixing ratio after individual pretreatment of each herbal medicine, and the raw material content of each component is 20-25 parts by weight of water, 20-25 parts by weight , 20-20 parts by weight of insects, 15-20 parts by weight of wukong, 15-20 parts by weight of scorpions, 15-20 parts by weight of recruitment, 12-17 parts by weight of sediment, 20-25 parts by weight of alum, 20-25 parts by weight of dried skin , Bongseonja 20~23 parts by weight, Counts 13~18 parts by weight, Triceps 13~18 parts by weight, Rhubarb 13~18 parts by weight, Phosphorus 13~18 parts by weight, Sprout barley 15~25 parts by weight, Noni 12~17 parts by weight Part, 12 to 17 parts by weight of sewage, 12 to 17 parts by weight of Angelica, 12 to 17 parts by weight of aloe or wood, 10 to 16 parts by weight of ginseng, 10 to 16 parts by weight of astragalus, 10 to 16 parts by weight of Cornus officinalis, 8 to 15 parts of sansa Hyperlipidemia, arteriosclerosis, thrombosis, angina pectoris, ischemic stroke and cerebral infarction, characterized in that it contains a content of 8 parts by weight, 8-15 parts by weight of gugamchocho, 20-25 parts by weight of rice water, and 8-10 parts by weight of cerebrum It can be achieved by agents for improving blood circulation that are for the prevention or treatment of diseases.

The agent for improving blood circulation according to the present invention may help in the prevention and treatment of diseases including hyperlipidemia and arteriosclerosis, cerebral thrombosis, angina pectoris, as well as improving blood flow, improving vascular sclerosis, improving vascular endothelial function, stroke, and It has dementia, palpitations, cramps and pain relief, so it can be used appropriately for patients who require it.

1 is a manufacturing process diagram of the herbal formulation of the present invention.
Figure 2 is a graph showing the results of analysis of blood clot decomposition of the mouse for improving blood circulation.
Figure 3 is a result of the TBA plate assay of herbal preparations.
Figure 4 is a graph showing the antihyperlipidemic effect of herbal preparations in hyperlipidemia-induced mice.
5 is a graph showing the results of an antithrombotic assay of a herbal preparation.

The present invention, in one aspect, water quality, insects, insects, konggong, scorpion, job openings, sediment, vitiligo, tendonpidae, bonja, county, triceps, rhubarb, doin, sprout barley, noni, sewage, Angelica, aloe or wood grain Effectively extract and mix various herbal herbal ingredients including ginseng, ginseng, cornus, sansa, gugamcho, jumi and cerebrum, and optimize or enhance the taste and functionality of the product by optimizing the taste of the product. It provides a preparation for improving blood circulation prepared by doubling and formulated with pills, tablets, capsules, injections, pouches, drinks, and beverages.

The present invention, in an additional aspect, is prepared by mixing and extracting the whole of the crude medicines after individual pre-treatment at a predetermined blending ratio, and the raw material content of each component is 20 to 25 parts by weight based on 400 parts by weight of the total raw materials. , 20~25 parts by weight of insects, 20~25 parts by weight of insects, 15~20 parts by weight of wukong, 15~20 parts by weight of scorpions, 15~20 parts by weight of recruitment, 12~17 parts by weight of sediment, 20~25 parts by weight of alum, 20-25 parts by weight of dried chili, 20-23 parts by weight of balsam, 13-18 parts by weight of count, 13-18 parts by weight of rhizome, 13-18 parts by weight of rhubarb, 13-18 parts by weight of sprouts, 15-25 parts by weight of sprout barley Parts, 12 to 17 parts by weight of noni, 12 to 17 parts by weight of sewage, 12 to 17 parts by weight of Angelica, 12 to 17 parts by weight of aloe or wood, 10 to 16 parts by weight of ginseng, 10 to 16 parts by weight of astragalus, 10 to 16 parts of cornus It provides a composition for improving blood circulation, characterized in that it contains a content of 8 parts by weight, 8-15 parts by weight of sansa, 8-15 parts by weight of Gugamcho, 20-25 parts by weight of rice, and 8-10 parts by weight of cerebrum.

Hereinafter, each individual component is demonstrated.

The water quality is dried from the leech (Hirudo nipponica Whitman) and the horse leech (Whitmania pigra Whitman) belonging to the family Lyraceae, soaked in water between summer and autumn, toss the soil, and then put into lime or alcohol to kill. Dries. Water quality acts on the cirrhosis and bladder, promotes blood circulation, eliminates blood stagnation, smooths menstruation, excretes urine well and aborts, and the hirudin component in leeches is known to have anticoagulant properties.

The beetle is a dried beetle (Eupolyphaga sinensis Walker) that lives in the ground with the asteraceae. It is caught in summer, soaked in boiling water or boiled in salt water and dried in the sun. Jachung is known to act on the heart, liver, and parenteral areas, and it is known to be effective in removing blood and enemies, making the meridians well, and connecting broken bones.

The worm (Tabanus trigonus) is an insect of the family Lepidoptera, so it is also called "to the iron" because it lives on the blood of cattle or animals. It is collected in July-August, dried to remove the legs and wings, and is medicated. It has a peculiar smell, a bitter taste and a bit salty, and a little cold. Efficacy is known to be effective for liver cirrhosis and cardiac stimulation, and has the effect of tracing, gueohyeol, and pain.

Ogong (蜈蚣, Scolopendra subspinipes mutilans) is a dried body of the king centipede, an animal belonging to the family Centipede, and is known for its antispasmodic, cardiovascular, antibacterial, anticoagulant, and anticancer effects. have.

As a pharmacological action on the cardiovascular system, the water-soluble tablets having removed the protein of the pores have a significant enhancement effect on the releasing atrial muscle contractility of guinea pigs, and also have an effect on the aged heart. Intravenous injection of the ugly extract to dogs results in a pronounced decrease in blood pressure, which is dose-dependent, and experiments with toad's vascular perfusion have also reported a marked increase in lower vascular perfusion. In addition, it is known that the extract of Wukong has the effect of inhibiting the action of thrombin on fibrinogen, thereby prolonging the blood clotting time.

Scorpion (全蝎, Buthus martensi) is an insect belonging to the scorpion family and is known to have anti-spasmodic, analgesic, and cardiovascular effects as a pharmacological action. Among them, in the pharmacological action against cardiovascular diseases, scorpion doxine (Buthatoxin) and its anti-convulsant peptides, which are constituents, have been found to have effects on the cardiovascular system. Scorpion toxin increases LVP of the anesthetized rabbit heart, dp/dt of the left ventricle and decreases heart rate. Increased cardiac contractility of the extracted guinea pigs and decreased heart rate or heart rate distress appears during the process. Anticonvulsant peptides, on the other hand, have reduced myocardial contractility, increased heart rate, and at the same time, accompanied by heartbeat.

Guin (medicinal name: Lumbricus, scientific name: Pheretima aspergillum (E. Perrier)) is used as an herbal medicine by drying earthworms. Traditionally, traditional Chinese medicine has been prescribed as a treatment for adult diseases such as arteriosclerosis, hypertension, diabetes, etc. In particular, it is described as being effective as an antipyretic agent, a stroke treatment, a blood accelerator, a hemiplegic agent, a joint treatment agent, a urination agent, a treatment for bronchial asthma, and a hypertension agent. In the present invention, it is preferable to use earthworms for living.

Cryptosis (蟬退, Cryptotympana pustulata) is an insect that belongs to the cicada family, and the peeled shell when the larvae of cicada and related insects emerge from the ground and become adults, and their pharmacology includes anticonvulsant action, sedative action, antipyretic action, analgesic action. , Immunosuppression and anti-allergic effects. As a double antispasmodic action, it has been reported that the sediment extract reduces the number of strychnine-induced spasticity and prolongs the time to death after convulsions of tetanus-induced mice and rabbits. In addition, it is known that the pickling extract has a pharmacological effect to improve vascular sclerosis.

Vitiligo is a medicinal material made of crystals processed from mineral alum (明礬石, Alumen). It is healed by taking pills in chronic gastrointestinal diseases and stomach and duodenal ulcers. It is effective. The contraction effect of dehydration of the woman's womb is obtained, and the effect of sterilization and convergence is obtained even under white. In addition, the antiseptic action is effective for psoriasis, scabies, and lymphadenitis of the skin. It is a drug that releases detoxification and hardening.

Dried dried bark is a dried bark bark that removes the most powerful blood of vegetable medicine. It causes blood loss and menstruation is stopped, causing birth defects. It is also used to keep the small intestine well and to get rid of roundworms.

Bongseonja is a seed of Bongseonhwa. The decoction of the seeds has uterine contraction and contraception (suppression of ovulation, oxygen contraction). The application is to use seeds for poisoning and menstruation in consent therapy. In addition, the stem is called golgolcho and is used for rheumatism, bruises, and menopause as a pain relief medicine, inflammatory medicine, and pain relief medicine. In the civilian, it is known that bee venom has the efficacy of swelling, pain relief, and edema, so it is treated for addictive joint pain, menstrual pain, lymphadenitis, and snake bite.

Peony (말린) is a dried perennial plant belonging to the Baguji family, which is the root of dried persimmon flowers and mountain hamper flowers. In autumn, the roots are washed with water and dried in the sun. Peony works on cirrhosis, parenteral, and menopause. It helps blood, cools pain and sweat, stops bleeding, lowers liver and makes urine better. In pharmacological experiments, the peoniflorin component exhibits sedative, analgesic, antispasmodic, antipyretic, anti-ulcerative, anti-ulcerative, hypotensive, coronary vasodilating effects, and the peonol component is sedative, antipyretic, analgesic, It is known to exhibit antispasmodic, anti-inflammatory and hemostatic effects.

Samchil-geun (Panax notoginseng <Burk.> F. H. Chen ebx.) is the root of the perennial plant Samchil (三七) belonging to the Araliaceae family. The main effect of the triceps muscle is a very important medicine for hemostasis applied to all bleeding both inside and outside the body. In particular, it is used for various bleeding symptoms, abdominal pain caused by blood stagnation, boils, pain relief, and a lot of blood after birth after smoothing the blood flow. In addition, it has the effect of lowering swelling and stopping the pain, so it swells with bruises and fractures, and treats painful symptoms, swelling or boils.

The rhubarb (Rheum palmatum Linne) is the root and rhizome of the Polygonaceae. Efficacy lowers excessively accumulated heat in the body, so it treats skin rash, constipation, and increased blood pressure and treats ocular hyperemia and pain. It is also used for initial symptoms of dysentery, and is effective for hemorrhage, nosebleed, and acute jaundice caused by heat, and it is widely used for symptoms such as severe fever, pain, and redness of the skin and hot water. In addition, if you put powder on frostbite that occurs frequently in winter and apply it to the affected area, the anti-inflammatory action is excellent and the wound is easily healed.

Doin (herbaceous name: Persicae semen, scientific name: Prunus persica (L.) Batsch) uses mature seeds of peaches to dry and use them as herbal medicine. Therefore, it can be applied to symptoms caused by most of the blood cells. At this time, in the present invention, it is preferable to crush the phosphorus and use it for living.

Sprout barley refers to a young plant that has grown to about 10-15 cm by sprouting on the barley. Sprout barley is known for its high nutritional content as well as its high content of inorganic components such as calcium, magnesium, and potassium, and its high content of vitamin B1 and vitamin C. Also, saponarine has antioxidant, anti-inflammatory, and anti-cancer properties ( Functional secondary metabolites, such as saponarin, lutonarin, and isovitexin, are increasing, and their industrial applicability is increasing as a material for health functional foods and pharmaceuticals. This ingredient serves to alleviate or neutralize the toxicity caused by herbal medicines, such as dried fruit. The sprout barley may be used for growing in the open field, or after immersing the barley in water for about a day, to remove moisture, to sow and germinate under conditions to prevent loss of moisture, to grow for 10 to 14 days to obtain. have.

Noni is called kanji or haepogeuk, and the scientific name is Morinda Citrifolia. It is the fruit of Southeast Asia Vietnam, East Malaysia, South Pacific, and Hawaii Titi. After drying, it is made of powder and sold, but it does not smell. It plays a role in catching chronic inflammation in our body. In other words, if there is inflammation or tissue damage in our body, the CRP level in the blood is slightly elevated, but normal if the CRP level is below 0.5mg/dl, there is no chronic inflammation, vasoconstriction, or loss of function of the vascular wall. There are noni as vegetable to lower, and inflammation is a precursor of cancer.

Healer heals healing, Ongjong, and five hemorrhoids, and heals the body's terrible wounds with thin, walled, and windy paths that have been many years old. It complements blood, strengthens tendons and bones, and replenishes essence. Blackens the hair, makes the face look good, and makes it live longer without getting old.

Angelica heals all wind diseases, all blood clots, all hurdles, heals evil blood, and produces new blood. It is mainly used for imperfection and impregnation of the wife and the wife, and it is used to heal various blood wounds and metal fittings to heal blood clots. The dysentery relieves the stomach ache, heals the oncology, reads chapter 5, and makes the flesh alive.

Acupuncture prevents the invasion of the pathogen and the spread of the wound when the wound is caused by the penetration of various pathogens, impact damage from the outside, or the invasion of insects during the growth process of the agar tree (Aquilaria agallocha Roxburgh). It refers to a tree in which resin generated by a protective action for protection is deposited. It cures feng shui or poison, removes bad energy and stops the end of the qi. Helps the new spirit, enhances sexual function, paralyzes with cold wind, vomits with gwakran, and diarrhea or rats is better.

Mokhyang is the root of the chrysanthemum perennial herb, Dangmokhyang, Cheonmokhyang, Wolseohyanghyang, and Geohyanghyang.It has a very unique scent, with chest and stomach pains of all kinds, 9 kinds of heartache, bloating and soreness with many years of cold, Heals the wall and punishment. It also stops diarrhea, kwakran and dysentery, releases poison and heals what is in vain. Prevent overheating, and make sure that the medications go to the intended destination.

Ginseng (人蔘, Panax ginseng) is a plant of the genus Agapiaceae and its roots are dried. Ginseng acts as a refresher, prevents the body from drying out, and causes blood. The properties are slightly warm, sweet, slightly bitter and non-toxic. It is a herbal medicine known to be effective in the central nervous system, immune enhancement, cardiovascular disease, endocrine system, and anti-aging. In particular, in cardiovascular diseases, ginseng has been found to have a strong cardiac action, a hamstring muscle ischemia action, an effect on blood vessels and blood pressure, and an effect on blood and hematopoietic systems.

Astragalus radix (Scientific name: Astragali radix, scientific name: Astragalus membranaceus Bunge) is used as a herbal medicine by drying the roots. It is sweet and slightly warm, and mainly acts on the spleen and lungs. Bokseungyang (작용陽) action to increase energy and lift energy, high-marked action to prevent sweating by firming the epidermis, and Takdokbae-nong (托毒排) that treats boils on the skin膿) It is used in various diseases of children and issuing edema to treat edema by smoothing action and water metabolism. It is used for symptoms such as a white or yellowish face due to weakness of the spleen, a sense of weakness in the limbs, and a weak feces. In addition, it is used when symptoms such as bleeding, powerlessness, and facial tinge appearing due to weak energy and weakening of the hematopoietic organs, resulting in bleeding, bleeding, subcutaneous hemorrhage, and uterine bleeding. It is known to be effective for symptoms such as hypothalamus, dehydration, long-term dehydration, and drowsiness because it has the effect of raising energy. At this time, in the present invention, it is preferable to use astragalus as a living.

Cornus officinalis Sieb. Cornus officinalis Sieb, a deciduous small tree belonging to the Cornus family. et. Dried fruits of Zucc., Cornus, Morroniside, Loganin, Tannin, Saponin, and other glycosides such as cornin, moronic acid, wine acid, malic acid, tartaric acid It contains a large amount of organic acids such as palmitic acid, oleic acid, linoleic acid, and vitamin A, and has antibacterial and antioxidant effects. According to Donguibogam and aroma-intensive, strong, strong, new, and novelty Efficacy such as reinforcement and convergence is known, and it is prescribed for symptoms of headache, tinnitus, sea sickness, and fever in oriental medicine.

Sansa is a fruit of hawthorn, which contains various organic acids, flavonoids, and vitamin C, which has the effect of strengthening vascular and cardiac functions, and is prescribed for postpartum abdominal pain, hangover, dry stomach, indigestion, and chronic diarrhea.

Gugamcho is a term that refers to licorice that has been slightly roasted and slightly weakened by roasting it. When baked and used, it becomes warm. It helps to protect the stomach and energy, and it is used to show the heart pounding symptoms due to lack of heartbeat, and the symptoms of physical discomfort and hysteria due to lack of heart and heartbeat, which is used to stabilize the mind. Depression, hysteria). In addition, it acts on the lungs, removes the phlegm, stops coughing, relieves cramping of the stomach and spasm of the limbs and relieves pain.

Beopmi refers to water (rice water) washed with rice, and neutralizes other ingredients added. In the present invention, the rice can be poured into rice with the same amount of water, soaked for about 30 minutes, washed, and repeated washing 3 times to collect and use it.

Dragon brain is a collection of lumps formed by cooling the liquid obtained by distilling water vapor by cutting the gin or stem or branch of the dragon brain (Dryobalanops aromatica Gaertner), dried in the shade, and acts on the heart, menopause and parenteral. The Dragon Brain clears the mind, lowers the heat, calms swelling and relieves pain.

Hereinafter, it will be described in connection with the preparation of a herbal preparation according to the present invention. 1 is a manufacturing process diagram of the herbal formulation of the present invention.

As shown in Fig. 1, 7 kinds of animal herbal medicines including water quality, insecticidal, larvae, scorpion, scorpion, guinea pig and sediment, and 1 mineral herbal medicine including alum and dried medicinal herb, balsamic, county, triceps, 18 kinds of herbal medicines, including rhubarb, doin, sprout barley, noni, sewage, Angelica, aloe or wood, ginseng, astragalus, cornus, sansa, gugamcho, jumi, and cerebrum, are prepared by extracting and mixing after individual pretreatment or raw materials A total of 400 parts by weight may be extracted and prepared at once.

More preferably, 7 kinds of animal herbal medicines, including water quality, insecticidal, larvae, wukong, scorpion, recruitment, and sediment, 1 mineral herbal medicine including vitiligo, and dried skin, balsamic, county, triceps, rhubarb, odontium , 18 kinds of herbal medicinal herbs, including sprout barley, Noni, Shou, Angelica, aloe or wood, ginseng, astragalus, cornus, hawthorn, citrus, rice, and cerebrum

a) a process of mixing the herbal medicines individually collected and pretreated and then naturally drying;

b) a step of individually pulverizing each of the dried herbal medicines to a particle size of 80 to 200 mesh;

c) a process of treating the pulverized herbal medicines under stirring at 70 to 80° C. for 2 to 10 minutes or by increasing at 100 to 105° C. for 2 to 10 minutes; And

d) a process of cooling the herbal medicines treated by ultra or thickening;

e) adding 10 to 25 times purified water based on the weight to the cooled herbal medicine to extract for 60 to 72 hours at a temperature of 0.5 to 0.6 atm and 70 to 80°C to obtain an extract;

f) filtering the foreign matter by filtering the extract of the process with a filter paper;

g) a process of filtering out foreign substances and adding 10 to 25 times purified water based on weight to the remaining solid content to extract for 60 to 72 hours at a temperature of 0.8 to 0.9 atm and 80 to 90°C to obtain an extract;

h) filtering the foreign matter by filtering the extract of the process with a filter paper;

i) The process of filtering foreign substances and concentrating the obtained filtrates to a solid brix of 40 or more for 4-8 hours under reduced pressure of -0.08 to -0.09 MPA at a temperature of 50 to 65°C:

j) a process of drying the concentrate obtained in the above process at a temperature of 55 to 65° C. under vacuum for 24 to 48 hours at low temperature; And

k) a step of pulverizing the dried product obtained in the above process into 120 to 200 mesh; can be extracted and used, and it may be desirable to formulate by mixing these extracts with purified or raw ingredients that do not require extraction.

In the above process, purified water or water may be used as the extraction solvent, and at least one selected from lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, or mixtures thereof may be used. As a lower alcohol having 1 to 4 carbons, methanol, ethanol, or the like can be used, and as the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol, and the like can be used. And, as the mixture, a mixture of water and lower alcohol, a mixture of water and polyhydric alcohol, a mixture of lower alcohol and polyhydric alcohol, or a mixture of water and lower alcohol and polyhydric alcohol may be used.

In more detail with regard to the extraction process of the herbal medicine, it is preferable that the pre-treated herbal medicine is first dried by hot air at a temperature of 55 to 65° C. for the efficiency of pretreatment.

Subsequently, the dried herbs are mixed in a predetermined ratio and then crushed to a particle size of 80 to 200 mesh. It is preferably ground to about 150 mesh using a pin mill or the like.

Then, the pulverized herbal medicines are treated under agitation at 70-80°C for 2-10 minutes or by increasing at a temperature of 100-105°C for 2-10 minutes, followed by cooling.

In the above process, the process of treating by candle or steam is for improving flavor and intake and preserving and expressing nutrients and medicinal ingredients as much as possible for 2-10 minutes at a temperature of 70-80°C. It is preferable to (i) or to increase and treat for 2 to 10 minutes at a temperature of 100 to 105°C. Here, Cho(炒) means roasting and Jeung(烝) means heating.

Subsequently, 10 to 25 times of purified water is added to the dried herbal medicine based on weight, and extracted for 60 to 72 hours at a temperature of 0.5 to 0.6 atm and 70 to 80°C to obtain an extract.

As a result of setting temperature conditions for standardization of the temperature conditions to be used for the extraction of herbal medicines, it was found that herbal herbal medicines are more efficient in extracting low or medium temperatures of 60℃~70℃ than in high temperature extraction. In addition, when looking at the content of various indicator components of herbal medicines, the extract extracted at 60℃~70℃ showed the highest content, and when extracted at 80℃ or higher, the content of the active ingredient tended to decrease.

In addition, the extraction time for the extraction of herbal medicines was the highest in the content of the active ingredient when extracted for 72 hours, and when it deviated, the extracted solid content and the active ingredient content tended to be lower.

On the other hand, the amount of the extraction solvent shows good results when extracted with 10 to 25 times of the experimental results for standardization of solvent usage, and considering energy efficiency and economic efficiency, it may be most effective to extract the solvent by adding it in 10 times. It is preferable to perform extraction pressure under conditions of 0.5 to 0.6 atmospheres.

The extract of the above process is filtered through a filter membrane or paper filter paper to filter out foreign substances and filter under no residue.

Subsequently, the foreign matter was filtered out, and 10 to 25 times of purified water was added to the remaining solids based on weight, followed by extraction at 0.8 to 0.9 atmospheres and a temperature of 70 to 80°C for 60 to 72 hours to obtain additional extracts, and similarly the extract was filtered paper Filtration with to filter out foreign substances and extract the active ingredients as much as possible.

Then, the foreign substances were filtered and the filtrates were combined and concentrated to a solid brix of 40 or more for 4 to 8 hours under reduced pressure of -0.08 to -0.09 MPA at a temperature of 50 to 65°C. As a result of various condition tests, the yield was best when concentrated under reduced pressure of -0.08 to -0.09 MPa using a reduced pressure concentrator.

It is preferable to dry the concentrate obtained in the above process at a temperature of 55 to 65° C. under vacuum for 24 to 48 hours at low temperature.

Next, as a process of pulverizing the dried product obtained in the above process into 120 to 200 mesh, it is preferably pulverized to about 150 mesh using a pin mill or the like.

Following the drying process or the grinding process, a sterilization process may be further included in accordance with product conditions.

In addition, alternatively, it is also possible to treat animal medicines and vegetable medicines separately. First, seven kinds of animal and minerals, including pre-treated water quality, insects, insects, pollen, scorpions, guinea pigs, and swine, are in a fixed ratio. After mixing,

a1) naturally drying the mixed herbal medicine;

b1) a step of individually pulverizing the dried animal medicinal herbs to a particle size of 80 to 200 mesh;

c1) a process of treating the pulverized animal medicinal herb by stirring for 2 to 10 minutes at 70 to 80° C. for 2 to 10 minutes under stirring or for 2 to 10 minutes at a temperature of 100 to 105° C.; And

d1) the process of cooling the animal herbal medicines treated by ultra- or thickening;

In addition, as an alternative, the herbal extracts, botanical extracts, county medicinal herbs, trigeminal roots, rhubarb, doin, sprout barley, noni, sageu, Angelica, aloe or aroma, ginseng, astragalus, cornus officinalis, hawthorn, citrus, juvenile and cerebrum Can be extracted individually or in combination,

a2) adding 10 to 25 times purified water based on weight to the dried herbal, extracting it for 60 to 72 hours at a temperature of 0.5 to 0.6 atm and 70 to 80°C to obtain an extract;

b2) filtering the foreign matter by filtering the extract of the process with a filter paper;

c2) a process of filtering out foreign substances and adding 10 to 25 times purified water based on weight to the remaining solid content to extract for 60 to 72 hours at a temperature of 0.8 to 0.9 atmosphere and 80 to 90°C to obtain an extract;

d2) a process of filtering foreign substances by filtering the extract of the process with a filter paper;

e2) The process of filtering foreign substances and concentrating the obtained filtrates to a solid brix of 40 or more for 4-8 hours under reduced pressure of -0.08 to -0.09 MPA at a temperature of 50 to 65°C:

f2) a process of drying the concentrate obtained in the above process at a temperature of 55 to 65° C. under vacuum at low temperature for 24 to 48 hours; And

g2) a step of pulverizing the dried product obtained in the above process into 120 to 200 mesh; it may be preferable to extract by.

The present invention has been made as a result of diligent efforts to develop a formulation with less side effects and improved blood circulation by using various herbal herbal medicines, and various herbal herbal ingredients and their combination ratio, effective extraction conditions of each component, and taste of the product, etc. There is a main characteristic to the optimization, and the composition ratio of the formulation according to the present invention is set by long-term research results in consideration of the effective dose and side effects of each component, and if it is outside the range of the ratio, Since it does not exhibit a desirable synergistic effect, the effect may be deteriorated.

More specifically, the above herbal medicines are based on the arrangement of Gunshin-jwasa (君臣佐使) on the basis of oriental medicine.'Gun' compared to wages is the ingredient that provides the most important medicinal properties, such as water quality, dried skin, and alum, This includes worms, worms, bee pollens, wukong, and scorpions, and the medicinal efficacy of'Gun(君)' can be maximized by'Sinjasa', which is likened to a subordinate. 'The rest of the ingredients are new to the left side of the body (강화佐使) to assist and enhance the efficacy of the military drug, or aim to relieve the toxicity and relieve symptoms associated with, or to guide the functional site for the desired functionality to the disease site, and various Chinese medicine These are the ones used to neutralize.

The blood circulation improving agent of the present invention can be effectively used as a medicinal or health functional food for the prevention and improvement of diseases including hyperlipidemia, arteriosclerosis, cerebral thrombosis, angina pectoris.

The agent for improving blood circulation of the present invention has no toxicity and side effects, but also improves blood flow, improves vascular sclerosis, improves vascular endothelial function, relieves cramping and pain, improves hyperlipidemia, improves arteriosclerosis, improves cerebral thrombosis, promotes blood flow, and promotes blood clotting. It is effective for patients with coronary artery disease such as angina pectoris, cardiac hyperactivity, sweating cold, short of breath and frequent fatigue by acting to improve blood vessel hardening such as relief and arteriosclerosis. Also, stroke and dementia during cerebral infarction recovery It can be particularly preferably used for diseases such as hemiplegia, poor eye circulation, and poor orbital symptoms, and those skilled in the art can easily identify individuals suspected of suffering from such diseases, conditions, and abnormalities.

The pharmaceutical and food formulations according to the present invention may be formulated and used in the form of oral or injectables such as pills, pouches or drinks according to conventional methods in the art.

Particularly, in the case of manufacturing as a pill, each material is mixed for 20 minutes or more in a mixer such as a double cone mixer, and then combined using a coalescing machine, and then exchanged using a conventional ventilation machine and dried at about 30° C., It can be prepared by coating and packaging it.

In addition, when coating, it may be desirable to dissolve hydroxypropylmethylcellulose in 95% alcohol and then coat it with a spray method, and it is preferable to dry it at 30±2°C for 1 hour or more and dry it to 5% or less for packaging.

The dosage of the pharmaceutical and food preparation of the present invention is appropriately set depending on the form of the preparation, the method of administration, the purpose of use and the age, weight, and symptoms of the individual who applies to it, and is not constant, but is generally an active ingredient contained in the preparation. The amount is from 25 mg to 5,000 mg/kg per adult per day. Of course, since the dosage varies depending on various conditions, an amount smaller than the above intake may be sufficient, or it may be necessary beyond the range.

The pharmaceutical preparation according to the present invention can be formulated in combination with a known pharmaceutical carrier. In general, the active ingredient is combined with a pharmaceutically acceptable liquid or solid carrier, and if necessary, a solvent, dispersant, emulsifier, buffer, stabilizer, excipient, binder, disintegrant, lubricant, etc. are added, and purified , Granules, powders, solids such as powders, capsules, liquids such as ordinary liquids, suspensions and emulsions. Moreover, it can also be set as a dried product that can be made into a liquid by adding a suitable carrier before use.

The pharmaceutical carrier can be selected according to the above dosage form and dosage form. For oral preparations, for example, starch, lactose, white sugar, mannitol, carboxymethylcellulose, corn starch, inorganic salts, etc. are used. In addition, in the preparation of an oral preparation, a binder, a disintegrating agent, a surfactant, a luster, a fluidity accelerator, a mating agent, a colorant, a fragrance, etc. may be further added.

The food preparation according to the present invention is a common adjuvant or additive, or sweetener, for example, honey, malt, glycerin, red ginseng, mushroom powder, cordyceps, grapefruit extract, licorice, pulverized medicinal leaf powder, western mountain lion extract, depending on the form of the desired preparation. , St. John's wort, selenium yeast, vitamin C, citric acid, nicotinic acid, sodium benzoate, aspartame, saccharin, pectin, malitol, sorbitol, xylitol, guar gum, skim milk and oligosaccharides. It can increase preference and aesthetics. It is appropriate to use these at about 50-80% based on the total weight of the formulation of the present invention.

<Example>

Hereinafter, the present invention will be described in more detail through examples. These examples are only intended to illustrate the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example 1: Preparation of a formulation for improving blood circulation, including a composite herbal herbal extract

Each commercially available herbal medicine, water quality 220g, jachung 220g, insect 230g, konggong 170g, scorpion 170g, job opening 170g, sediment 150g, alum 220g, dried dried 220g, seaweed 210g, county medicinal 150g, triceps 150g, rhubarb 150g, doin 150g, 200 g of sprout barley, 150 g of Noni, 150 g of sewage, 150 g of Angelica, 150 g of aloe, 130 g of ginseng, 130 g of astragalus, 130 g of Cornus officinalis, 100 g of Sansa, 100 g of Gugamcho, 100 g of Korean medicinal herbs and 90 g of Chinese herbal medicines are mixed and dried naturally. It was pulverized to a particle size of 150 mesh, and the pulverized herbal medicine was stirred at a temperature of 80° C. for 5 minutes under stirring, and then left to dry at room temperature. The rice was poured into 500 g of rice, 500 g of water, soaked for about 30 minutes, washed, and then repeated 3 times to collect the washed and used 220 g.

50 liters of purified water was added to the dried herbal mixture, followed by extraction at a temperature of 0.5 at 80° C. for 72 hours. The filtrate was collected by filtering the extract with a paper filter paper, and 50 liters of purified water was added to the remaining solids, followed by secondary extraction at a temperature of 0.9 at 80° C. for 72 hours. The extract was filtered through paper filter paper, the filtrates were combined, and then concentrated under reduced pressure of -0.08 MPA at a temperature of 80°C for 4 hours. The solid content of brix was 50. Subsequently, the concentrate was dried under vacuum at a temperature of 55° C. for 24 hours at a low temperature to prepare a formulation for improving blood circulation, including a complex herbal herbal extract.

Example 2: Pill preparation

Formulation for improving blood circulation of Example 1 (hereinafter, based on weight), malt powder 23%, shiitake mushroom powder 3%, citric acid 7%, sorbitol 10%, xylitol 35% and oligosaccharide 15% in a double cone mixer for 25 minutes After mixing for a while, coalescing using an associative group, and then using an associating machine, drying at about 30° C., dissolving hydroxypropylmethylcellulose in 95% alcohol, coating with a spray method, and 30 ㅁ on a dryer The product was dried at 2° C. for 1 hour to 5% or less, and then packaged (PS) to prepare a pill having a content of 3.5 g.

Example 3: Tablet formulation

Blood circulation improving agent (Example 1) 25 mg

Lactose 60 mg

Starch 15 mg

Magnesium stearate 15 mg

Crystalline cellulose 15 mg

Tablets may be prepared by conventional methods, and one preferred embodiment is in the form of tablets or soft capsules having a conventional enteric coating (eg, hydroxypropylmethyl cellulose phthalate), sugar coating or coating.

Example 4: Preparation of beverage

20 g of citric acid, 17 g of aspartame and 30 g of vitamin C were added to 100 g of the formulation for improving blood circulation obtained in Example 1, and the rest was added as purified water to prepare 10 l of beverage and pasteurized. As a result, the beverage of the present invention obtained a good response in terms of mouthfeel, taste, and comprehensive evaluation with the conventional beverage product.

Example 5: Preparation of injection

Blood improvement agent of Example 1 20 mg

Mannitol 180 mg

2800mg sterile distilled water for injection

It is prepared with the above-mentioned ingredient content per ampoule (3 ml) according to the preparation method of a conventional injection.

Example 6: Preparation of pouch agent

A pouch was prepared by an automatic filling machine so that 40 g of the blood circulation improving agent obtained in Example 1 per pouch contained 10 g of vitamin C, 10 g of aspartame, and 50 g of purified water.

Example 7: Acute toxicity test

Toxic experiments on the experimental animals of the blood circulation improving agent according to Example 1 were performed and the presence or absence of the toxicity was observed. Experimental animals were 4 and 5 weeks of age, body weight of 105±4g, male and 95±3g of female SD rats, 60 rats were used and tested using distilled water as a negative control as a formulation of the present invention. First, the rats are about 1 in a laboratory breeding box with a temperature of 22±2°C, a relative humidity of 53±2%, a contrast cycle of fluorescent lighting (09:00 lit-18:00 off), and illuminance conditions of 150-300 Lux. After purifying over a period of a week or so, only healthy animals are selected, divided into groups to have an average weight, and administered orally for 14 days in an amount of 20 ml/kg once a day, followed by changes in general conditions and symptoms of addiction , Mobility, appearance, autonomic nerve, weight change, and presence or absence of dead animals.

According to the results of the experiment, although there was a change within 5% in body weight during the experiment period, there was no significance, and a dead animal was not observed even though the highest concentration of 20 ml per kg, which is the maximum amount that can be administered to the test animal, was selected. As the lethal dose was not calculated, LD ₅₀ was found to be 20 ml/kg BW or more, and no specific lesions were observed at the time of autopsy or autopsy. Therefore, it was found that the formulation was non-toxic.

Example 8: Blood clot digestion assay

After receiving blood, 0.5 ml each was contained in several tubes that were previously measured for mass, and reacted at 37° C. for 45 minutes to form a clot, and after forming the clot, the serum layer was completely removed and the clot mass was measured. The herbal preparation of Example 1 was reacted therewith to confirm the blood clot decomposition effect.

The results are shown in FIG. 2. Figure 2 is a result of the mouse blood clot decomposition analysis of the blood circulation improving agent according to the present invention. The control group included only the phosphate buffer solution, and the blood circulation improving agent contained 100 μg.

In the mouse blood clot decomposition analysis, 100 μg of an herbal medicine extract (Example 1) degraded fibrin clot by about 87%. Mouse blood clots Through the in vitro test results used, it was possible to confirm the thrombolytic activity capable of directly decomposing clots formed in the blood of mice of herbal herbal preparations.

Example 9: lipolysis effect

(1) TBA plate essay

10 μl of the herbal extract preparation of Example 1 was made by depositing a 3 mm ring on a TBA (tributyrin agar) plate containing 0.1 ml of tributyrin and 0.1 g of agar, and then reacted at 37° C. for 12 hours to disassemble the clear zone. Confirmed.

The results of performing the TBA (tributyrin agar) plate assay of the herbal extract formulation are shown in FIG. 3. 3 is a result of the TBA plate assay of the herbal extract formulation. The buffer solution contained only the phosphate buffer solution, and the herbal extract formulation contained 10 μg. From the above results, 10 μg of herbal extract formulation was able to confirm the clean zone around the ring by decomposing lipids. Through this experiment, it was confirmed that the herbal extract formulation had an effect of directly decomposing lipid components.

(2) Lipid-degrading activity assay using lipolytic enzyme synthesis substrate

Enzyme activity was measured by a colorimetric method (Stuer et al., 1986) using 4-nirtophenyl palmitate and 4-nitrophenyl butryrate. 1 mg of the herbal extract preparation of Example 1 was dissolved in 1 ml of 50 mM phosphate buffer (pH 6.0), 10 μl of the substrate solution (100 mM p-nitrophenyl butyrate (pNPB) dissolved in 2-propanol) was added, and then 10 minutes. During the reaction at 30 ℃. After centrifugation at 2000 x g for 5 minutes at 4°C, the supernatant was recovered to measure activity. Lipidase activity was confirmed by measuring the amount of p-nitrophenol produced at 410 nm. The definition of 1 unit of enzyme refers to the amount of enzyme that produces 1 μM of p-nitrophenol per minute. The results are shown in Table 1 below.

temperament Lipolytic activity (mU/ml) p -nitrophenyl butyrate 3640 p -nitrophynyl palmitate 1470

As can be seen from the results of Table 1, the herbal extract formulation exhibited lipid decomposition activity of 3640 mU/ml on the p- nitrophenyl butyrate substrate and 1470 mU/ml on the p- nitrophynyl palmitate substrate. Through this experiment, it was confirmed that the herbal extract preparation had the same activity as lipase, an enzyme that directly degrades lipid components, and confirmed high activity in each substrate.

Example 10: Antihyperlipidemic effect test

Blood was collected through the tail vein of a laboratory animal rat, and the collected blood was left at room temperature for about 30 minutes, and then centrifuged at 3000 rpm for 20 minutes to extract serum and stored at -30°C until analysis. Anticholesterolemia effect was analyzed and Friedewald was analyzed by measuring total cholesterol, TG (triglyceride), HDL-C (high density lipoprotein cholesterol), LDL-C (low density lipoprotein cholesterol), and VLDL-C (ultra low density lipoprotein cholesterol). It was analyzed by the concentration calculation method using the formula.

Fig. 4 shows the results of analyzing the antihyperlipidemic effect by dieting the feed containing the herbal extract preparation (Example 1) in the rats that induced hyperlipidemia. 4 is a graph showing the antihyperlipidemic effect of the herbal extract preparation in hyperlipidemia-induced mice. C is the plasma of normal rats as a control, NC is the plasma of hyperlipidemia-inducing rats as a negative control, and the sample is the plasma of a hyperlipidemic-induced rats fed with a herbal extract (1 mg) as an experimental group.

As can be seen from the results of FIG. 4, the total cholesterol level of the control group (N, normal diet) fed only with normal feed was 107 mg/dL, and the HDL-cholesterol (high density lipoprotein cholesterol) value was 68.3 mg/ dL and LDL-cholesterol (low density lipoprotein cholesterol) levels were 15.3 mg/dL, VLDL-cholesterol (ultra low density lipoprotein cholesterol) levels were 22.4 mg/dL, and TG (triglyceride) levels were 110.3 mg/dL.

The total cholesterol level of the negative control group (NC, hyperlipidemia-inducing group) fed only with high-cholesterol feed was 140.2 mg/dL, HDL value was 52.3 mg/dL, LDL value was 38 mg/dL, VLDL value was 23.5 mg/dL , TG (triglyceride) level was measured to be 132.3 mg/dL. Accordingly, as a result of comparing total cholesterol, HDL, LDL, VLDL, and TG of the normal group fed only with normal feed, total cholesterol increased by about 31% and HDL decreased by about 23.4%. In addition, LDL increased by about 148.3% and VLDL and TG increased by 4.9% and 19.9%, respectively.

In the results of the sample group in which diets supplemented with herbal extracts were fed to rats induced with hyperlipidemia, the total cholesterol level was 109.2 mg/dL, the HDL level was 73.6 mg/dL, the LDL level was 21.7 mg/dL, and the VLDL level was 16.8. The mg/dL and TG levels were determined to be 92.7 mg/dL. Accordingly, when the control group fed with the normal feed and the negative control group fed with the high cholesterol feed were compared with the total cholesterol, HDL, LDL, VLDL, and TG, the total cholesterol, VLDL, and TG levels were restored to normal values, and the LDL was While it was significantly reduced from 38 mg/dL to 21.7 mg/dL, the HDL level was elevated to confirm the recovery effect by the diet of the herbal preparation.

In addition, AI (Arteriosclerosis Index, Atherogenic index (total cholesterol-HDL cholesterol) / HDL-cholesterol), HDL/LDL ratio, TC/HDL ratio was calculated and compared, and the results are shown in Table 2.

Item C NC Herbal medicine AI 0.56 1.67 0.48 Total cholesterol/HDL ratio 1.56 2.68 1.48 HDL/LDL ratio 4,46 1.37 3.39

As a result, as a result of dieting for 14 days the feed containing the herbal extract formulation in rats that induced hyperlipidemia, it was confirmed that it had the effect of improving blood circulation (prevention of hyperlipidemia) in the results of all 5 items, arteriosclerosis index, Total The cholesterol/HDL ratio and HDL/LDL ratio also confirmed the significance of the antihyperlipidemic effect.

Example 11: Analysis of hemolytic activity using Rodent blood

Hemolytic activity analysis is an antithrombotic efficacy evaluation method using a blood clot derived from the blood of a laboratory animal through an anti-thrombotic activity assay method. This is an experiment to evaluate the thrombus removal efficacy of a sample by analyzing the amount of thrombus and the reaction solution.

After the reaction of the herbal extract and blood clot for 1 hour, the weight of the remaining blood clot and the absorbance of the reaction solution were measured to analyze the thrombus removal efficacy of the herbal extract.

Blood clots were formed by spontaneous clotting of 300 μL of blood clots from mouse blood in vitro. The digestion of blood clot was evaluated by incubating a sample of herbal extract preparation, positive control t-PA (20 U), and saline treated clot at 37° C. for 1 hour. After incubation, antithrombotic activity was evaluated by color development, and the absorbance of the supernatant (blood clot + saline(con), herbal extract preparation or t-PA) was read as absorbance at 410 nm. 5 is a graph showing the results of the antithrombotic assay of the herbal extract formulation.

In the herbal extracts of the experimental group, absorbances of 0.430, 0.715, 1.120, 1.220, and 1.382 at 410 nm were confirmed in order at concentrations of 10 μg, 20 μg, 50 μg, 100 μg, and 200 μg. The absorbance of the negative control treated with physiological saline instead of the sample was 0.403, and the positive control, t-PA, was 0.463 at 10 U concentration, 0.880 at 20 U, and 1.379 at 30 U.

The absorbance of the reaction solution of the 10 μg herbal extract was slightly lower than that of the 20 U t-PA, but the absorbance of the reaction solution of the 50 μg to 200 μg herbal extract was higher than that of the 10 U t-PA.

Through the antithrombotic activity assay, after the formation of blood clots in experimental animals, the herbal extract preparation had excellent thrombolytic ability and showed a higher thrombi removal capability than 10U t-PA in extracts of 20 μg or more. Therefore, it seems that the herbal medicine extract acts on the blood of experimental animals and can expect the effect of coagulation control and blood circulation improvement through prevention of excessive thrombus formation.

Example 12: Platelet aggregation inhibition ability test

Platelet rich plasma (PRP) and platelet poor plasma (PPP) were separated from the blood obtained by anesthetizing male rats (200-300 g) with ether, and the antithrombotic effect was measured using an aggregometer. In order to examine the ability to inhibit platelet aggregation of the herbal extract preparation according to the present invention, after adding the saline administration group and the preparation ug/ml of Example 1 according to the present invention as a control, thrombin (thrombin-100 unit/ml) was treated, The results are shown in Table 3 below.

Concentration (ug/ml) Platelet aggregation inhibition rate (%) Control 0.01±0.45 Example 1 86.4±0.27

As can be seen from the results of Table 3, the efficacy when treated with thrombin inhibited 86.4% and showed excellent antithrombotic effects.

Example 13: Influence test on coronary blood flow

Cholesterol diet was performed for 2 weeks in a 6-week-old rat, and the control group was administered orally with saline and the herbal herbal extract preparation of Example 1 according to the present invention once daily. Two weeks later, the rats were anesthetized with pentobarbital and the heart was removed while perfusion of the heart with a Krebs-Henseleit physiological solution at 37°C. After 30 minutes, when the extracted heart was stable, the change in coronary blood flow was measured through an electric blood flow meter, and the results are shown as mean±S.E.M. in Table 4 below. Statistical significance level (P<0.05) compared to control.

Concentration (mg/kg) Coronary blood flow (ml/min) Control 18.8±1.91 Example 1 29.6±1.21

As can be seen from the results in Table 4, the control group showed 18.8 ml/min for coronary blood flow, whereas the herbal herbal preparation of the present invention showed a significantly increased concentration-dependent increase to 29.6 ml/min.

Example 14: Effect test for ischemic stroke

The effect of inhibiting ischemic stroke of the herbal preparation of Example 1 was confirmed. As a test animal, male Spargue-Dawley rats weighing 280-300 g were used. To confirm the inhibitory effect of ischemic stroke, the experimental animal was anesthetized with a mixed gas of N ₂ O and O ₂ mixed with a volume ratio of 7:3 and 3% isoflurane, and then the experimental animal's common artery (common) The carotid artery and the external carotid artery are ligated, and a 19 mm probe (approximately 2.1 cm of 4-0 surgical nylon thread from the branch of the internal carotid artery) is burned and rounded. Insertion) into the right sheath endovascular middlecerebral artery occlusion to induce local cerebral ischemia. Thereafter, the blood flow in the middle cerebral artery was blocked for 90 minutes to maintain an ischemic condition, and then the surgical site was exposed again to remove the probe and reperfused. The incised skin of the reperfused laboratory animal was completely sealed using a surgical suture, and the rectal body temperature of the laboratory animal was maintained continuously so as not to fall below 36-37°C using the hot pad used during the entire experimental process. The sample of Example 1 was orally administered twice at 3 hours and 8 hours after inducing ischemia (100 mg/kg each). At this time, no sample was treated in the control group. Next, after reperfusion of the middle cerebral artery, the animal was killed 24 hours to extract the brain of the experimental animal, and then a brain slice was formed to a thickness of 2 mm using a brain shape frame, and then 2,3,5-triphenyltetrazolium chloride ( 2,3,5-triphenyltetrazonlium chloride, TTC) solution was immersed at 37°C for 20 to 30 minutes. After confirming the color reaction, the brain slices were fixed in 4% paraformaldehyde, and the infarction site was analyzed using an image analysis system. The infarct area due to ischemia is expressed as a percentage (%) of the total brain volume, and the results are shown in Table 5.

As a positive control, MK-801{(+)-5-methyl-[0091] 10,11-dihydro-5H dibenzo[a,d]-cyclohepten-5,10-imine maleate, which is an excitatory neurotoxic inhibitor NMDA receptor blocker } Was used.

sample Infarct volume(%) Example 1 9.12 Control 25.7 Positive control 9.65

As shown in Table 5, in the cerebral infarction region of the middle cerebral artery occlusion/reperfusion injury model brain, the sample of Example 1 was found to have a significantly reduced volume of the infarct area due to ischemia compared to the positive control group, thereby confirming excellent brain tissue protection.

Example 15: Influence test on the change in the degree of neurological deficiency

Influence on the effect of local cerebral ischemia on the change in the degree of neurological deficit according to the middle cerebral artery occlusion was investigated.

The male rats (280-300 g) were administered orally once daily to the saline administration group and the herbal medicine preparation group of Example 1 according to the present invention as a control group. Thereafter, the cervical incision was made while maintaining the pentobarbital anesthesia at 37°C to separate the right common carotid artery, the internal carotid artery, and the external carotid artery. The total carotid artery and the external carotid artery were ligated and the probe was inserted into the internal carotid artery from the branch of the internal and external carotid arteries, and the base of the middle cerebral artery was closed by ligation just above the insert.

The surgical site was sutured and placed in a single breeding box until anesthesia broke. After 30 minutes, the neurological defect was observed by the movement of the tail. After closing the middle cerebral artery for 120 minutes, the thread connected to the tip of the probe was recirculated by pulling out about 1 cm.

The following Table 6 is a result of examining the degree of neurological deficiency of the forelimbs, and the semi-incomplete paralysis symptoms were observed, and the degree of paralysis was divided into 0, 1, 2, 3, and 4 grades.

Middle cerebral artery occlusion surgery Hypoxia Recirculation Control 2.81±0.21 2.77±0.23 2.73±0.52 Example 1 1.78±0.35 2.02±0.27 1.72±0.35

In the case of the control group, the grades immediately after surgery, hypoxia, and recirculation were 2.81±0.21, 2.77±0.23, and 2.73±0.52, respectively. In Example 1, there were almost no changes in grades to 1.78±0.35, 2.02±0.27, and 1.72±0.35.

The following Table 7 is the result of examining the degree of neurological deficit in the hind limb and was divided into 2 grades of 0 and 1 for scoring. The administration group according to the invention was significantly improved compared to the control group.

Middle cerebral artery occlusion surgery Hypoxia Recirculation Control 0.51±0.43 0.24±0.26 0.61±0.42 Example 1 0.10±0.33 0.01±0.21 0.02±0.33

The herbal medicine preparation for improving blood circulation according to the present invention improves blood circulation, improves hyperlipidemia, tests for treatment for coronary artery disease, tests for treatment for heart disease improvement, tests for treatment for brain diseases, tests for treatment for hyperlipidemia As confirmed through, it was confirmed that the pharmacological effect is excellent.

From the above results, blood circulation improvement effect and anti-hyperlipidemic effect were confirmed, and not only can it be applied to medicine or health-oriented food, but also developed into the age of aging, which is emerging as a social problem by using herbal medicine extract material developed. Development of a customized health-oriented silver food product for use, as a product for maintaining a healthy life such as prevention and suppression of degenerative physical functions, and prevention centered on continuous intake without side effects aimed at preventing cardiovascular diseases It can also be used in the health care industry.

Claims (5)

Water Quality, Insect, Insect, Wukong, Scorpion, Job, Seon, Vitiligo, Dried Peel, Balsam, Peony, Triceps, Rhubarb, Doin, Sprout Barley, Noni, Sewage, Angelica, Aloe or Musk, Ginseng, Hwanggi, Cornus, Sansa , Gugamgamcho, containing rice water and hot water extract of cerebral,
The raw material content of each component is 20 to 25 parts by weight of water, 20 to 25 parts by weight of insects, 20 to 25 parts by weight of insects, 15 to 20 parts by weight of scorpions, 15 to 20 parts by weight of scorpions, 15 to 20 parts by weight of recruitment 12 to 17 parts by weight, alum 20 to 25 parts by weight, dried dried skin 20 to 25 parts by weight, balsamic seeds 20 to 23 parts by weight, earl counts 13 to 18 parts by weight, triceps 13 to 18 parts by weight, rhubarb 13 to 18 parts by weight, 13-18 parts by weight, 15-25 parts by weight of sprout barley, 12-17 parts by weight of noni, 12-17 parts by weight of sewage, 12-17 parts by weight of Angelica, 12-17 parts by weight of aloe or wood, 10-16 parts by weight of ginseng Part, characterized by including the content of 10-16 parts by weight of astragalus, 10-16 parts by weight of Cornus officinalis, 8-15 parts by weight of Sansa, 8-15 parts by weight of Gugamcho, 20-25 parts by weight of rice water and 8-10 parts by weight of cerebrum A preparation for improving blood circulation for preventing or treating diseases of any one of hyperlipidemia, arteriosclerosis, thrombosis, angina pectoris, ischemic stroke and cerebral infarction.
The method according to claim 1, wherein the agent for improving blood circulation
a) a process of mixing and drying the herbal medicines individually collected and pretreated;
b) a step of individually pulverizing each of the dried herbal medicines to a particle size of 80 to 200 mesh;
c) a process of treating the pulverized herbal medicine under stirring at a temperature of 70 to 80°C for 2 to 10 minutes, or increasing or treating at a temperature of 100 to 105°C for 2 to 10 minutes; And
d) a process of cooling the herbal medicines treated by ultra or thickening;
e) adding 10 to 25 times purified water based on weight to the cooled herbal medicine to extract for 60 to 72 hours at a temperature of 0.5 to 0.6 atm and 70 to 80° C. to obtain an extract;
f) filtering the foreign matter by filtering the extract of the process with a filter paper;
g) a process of filtering out foreign substances and adding 10 to 25 times purified water based on weight to the remaining solid content to extract for 60 to 72 hours at a temperature of 0.8 to 0.9 atm and 80 to 90°C to obtain an extract;
h) filtering the foreign matter by filtering the extract of the process with a filter paper;
i) The process of filtering the foreign substances and concentrating the obtained filtrates to a solid brix of 40 or more for 4-8 hours under reduced pressure of -0.08 to -0.09 MPA at a temperature of 50 to 65°C:
j) a process of drying the concentrate obtained in the above process at a temperature of 55 to 65° C. under vacuum at low temperature for 24 to 48 hours; And
k) a process for pulverizing the dried product obtained in the above process into 120-200 mesh; improved blood circulation for preventing or treating diseases of hyperlipidemia, arteriosclerosis, thrombosis, angina pectoris, ischemic stroke and cerebral infarction, characterized by being extracted by Dragon formulation.
The method according to claim 1,
The preparation is for the prevention or treatment of any disease of hyperlipidemia, arteriosclerosis, thrombosis, angina pectoris, ischemic stroke and cerebral infarction, characterized in that it is in the form of oral, pill, tablet, capsule, injection, pouch, drink or beverage. Yongin blood circulation improvement agent.
The method according to claim 1,
The agent prevents or improves diseases including hyperlipidemia, arteriosclerosis, cerebral thrombosis, angina pectoris, stroke, dementia, palpitations and pain, cerebral infarction, coronary artery disease, or improves vascular sclerosis, improves blood flow, improves vascular endothelial function, and peripherals. A drug for improving blood circulation for preventing or treating diseases of any one of hyperlipidemia, arteriosclerosis, thrombosis, angina pectoris, ischemic stroke, and cerebral infarction, which are helpful in improving vascular circulation.
The method according to claim 1,
The agent is a hyperlipidemia, arteriosclerosis, thrombosis, angina pectoris, ischemic stroke, which helps to prevent aging of blood vessels, improve skin elasticity, restore memory, or prevent a variety of blood vessels in advance. A drug for improving blood circulation for preventing or treating any one of cerebral infarctions.

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