KR102119622B1 - Cosmetic composition comprising the extract of fermented Sorghum Bicolor sprout for skin anti-wrinkle effect and producing method thereof - Google Patents
Cosmetic composition comprising the extract of fermented Sorghum Bicolor sprout for skin anti-wrinkle effect and producing method thereof Download PDFInfo
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- KR102119622B1 KR102119622B1 KR1020180041601A KR20180041601A KR102119622B1 KR 102119622 B1 KR102119622 B1 KR 102119622B1 KR 1020180041601 A KR1020180041601 A KR 1020180041601A KR 20180041601 A KR20180041601 A KR 20180041601A KR 102119622 B1 KR102119622 B1 KR 102119622B1
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- sorghum
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- sprout
- fermentation
- fermentation broth
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
본 발명은 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물 및 그의 제조방법에 관한 것이다.
본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물은 수수새싹 추출액을 사카로미세스로 발효시켜 수득한 수수새싹 발효액 또는 이의 발효 추출액을 함유한다.
상기한 구성에 의해 본 발명은 수수새싹 추출액을 사카로미세스로 발효한 발효액 또는 상기 발효액을 추출한 발효 추출액을 수득하여 피부 주름개선 효과가 우수하고 피부 자극에 대한 부작용이 없다.The present invention relates to a cosmetic composition for improving skin wrinkles and a method for manufacturing the same, comprising a fermentation broth of a sorghum sprout extract or a fermentation extract thereof as an active ingredient.
The cosmetic composition for improving skin wrinkles containing the fermentation broth of the sorghum sprout extract according to the present invention or the fermentation extract thereof as an active ingredient contains the sorghum sprout fermentation broth obtained by fermenting the sorghum sprout extract with Saccharomyces or its fermentation extract.
According to the above-described configuration, the present invention obtains a fermentation broth obtained by fermenting the sorghum sprout extract with Saccharomyces or a fermentation extract obtained by extracting the fermentation liquid, and has excellent skin wrinkle improvement effect and no side effects on skin irritation.
Description
본 발명은 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물 및 그의 제조방법에 관한 것으로서, 더욱 상세하게는 수수새싹 추출액을 사카로미세스로 발효한 발효액 또는 상기 발효액을 추출한 발효 추출액을 수득하여 피부 주름개선 효과가 우수하고 피부 자극에 대한 부작용이 없는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물 및 그의 제조방법에 관한 것이다. The present invention relates to a cosmetic composition for improving skin wrinkles containing a fermentation broth of a sorghum sprout extract or a fermentation extract thereof as an active ingredient and a method for manufacturing the same, and more specifically, a fermentation broth or a fermentation broth fermented with a sorghum sprout extract to Saccharomyces It relates to a cosmetic composition for improving skin wrinkles and a method for preparing the skin wrinkles containing the fermentation solution of the sorghum sprout extract or the fermentation extract thereof having no side effects on skin irritation by obtaining a fermentation extract obtained by extracting and having no side effects on skin irritation.
또한, 본 발명은 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 화장료 조성물을 제공함으로써, 섬유아세포 증식 촉진, 분자내 Collagen, TIMP-1, Tropoelastin의 유전자 발현과 MMP-1의 유전자 발현을 억제하여 피부의 주름을 개선하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물 및 그의 제조방법에 관한 것이다. In addition, the present invention provides a cosmetic composition containing a fermentation broth of a sorghum sprout extract or a fermentation extract thereof as an active ingredient, thereby promoting fibroblast proliferation, gene expression of Collagen, TIMP-1, Tropoelastin in the molecule and gene expression of MMP-1. It relates to a cosmetic composition for improving skin wrinkles and a method for manufacturing the same, using the fermentation broth of the sorghum sprout extract to improve the wrinkles of the skin by inhibiting or the fermentation extract thereof as an active ingredient.
화장품의 기능은 청결과 미적 기능뿐만 아니라, 수분, 미세먼지, 공해 물질, 스트레스, 자외선, 기타 등의 외적인 요인과 면역세포의 기능저하, 세포 활성의 저하 등의 내적 요인에 기인한 자유 라디칼(Free Radical), 활성산소, 과산화물 등에 의한 단백질, 핵산, 세포막 지질 파괴 등의 피부 노화의 발생을 차단하거나 억제하는 것이다. The function of cosmetics is not only cleanliness and aesthetic function, but also free radicals due to external factors such as moisture, fine dust, pollutants, stress, ultraviolet rays, etc., and internal factors such as deterioration of immune cells and decrease in cell activity. Radical), which blocks or suppresses the occurrence of skin aging such as destruction of proteins, nucleic acids, and cell membrane lipids by free radicals and peroxides.
또한, 소비자의 고기능성 화장품에 대한 욕구를 충족시키기 위한 미백, 피부주름 개선 및 억제, 그리고 자외선 차단 기능을 갖는 화장품들이 계속해서 개발되고 있다. 그 중에서도 특히 피부주름 개선 및 억제 제품은 피부를 젊게 유지하려는 소비자들로부터 많은 관심을 받고 있다.In addition, cosmetics that have whitening, skin wrinkle improvement and suppression, and UV protection to meet consumers' desire for high-functional cosmetics are continuously being developed. Among them, skin wrinkle improvement and suppression products have received a lot of attention from consumers who want to keep their skin young.
일반적으로 사람의 피부는 나이가 들어감에 따라 내적 또는 외적인 환경 요인에 의하여 수분의 소실과 탄력성 저하, 피부색과 윤기를 잃고 거칠어진다. 이것을 피부 노화라고 하며, 피부의 주름이 발생되고, 기미, 주근깨 등이 많아지는 과정이다.In general, as a person's skin ages, it loses moisture and decreases elasticity, loses skin color and shine, and becomes rough due to internal or external environmental factors. This is called skin aging, and it is a process in which wrinkles of the skin are generated and spots and freckles are increased.
피부에 노화가 진행되면 피부의 구성 성분인 표피, 진피 및 피하조직층의 두께가 얇아지게되고 피부에 탄력을 주는 세포외 기질(Extracellular matrix; ECM) 성분이 변화하게 된다.As the skin ages, the skin's constituent epidermis, dermis, and subcutaneous tissue layers become thinner and the extracellular matrix (ECM) component that gives elasticity to the skin changes.
콜라겐은 세포외 기질 중 전체 피부 건조중량의 약 70~80%를 차지하여 엘라스틴(elastin, 탄력섬유), 프로티오글리칸(proteoglycans), 글루코스아미노글리칸(glucosaminoglycan), 라미닌(laminin), 파이브로넥틴(fibronectin)과 함께 탄력을 유지하고 있으나 나이가 들면서 점차 콜라겐의 생성능력도 급격히 낮아지고, 피부세포에 산화가 진행되면서 피부가 탄력을 잃고 주름이 과도하게 형성되면서 노인성 피부로 변화되어 간다.Collagen accounts for about 70 to 80% of the total dry skin weight in the extracellular matrix, elastin (elastic fibers), proteoglycans, glucosaminoglycan, laminin, and fibro It maintains elasticity with fibronectin, but as it ages, the ability to produce collagen gradually decreases, and as skin cells become oxidized, the skin loses elasticity and wrinkles form excessively, resulting in aging skin.
아이 때는 체내에 콜라겐(collagen)이 많아서 온몸이 매끌매끌하지만, 나이가 들면 콜라겐이 부족하거나 질이 저하되어 피부는 탄력을 잃고 주름이 생기게 된다. 피부의 주름은 피부의 수분 함유량과 콜라겐의 함유량 그리고 면역 기능 등의 여러 요인에 영향을 받으며, 이 중에서도 가장 큰 영향을 미치는 것이 콜라겐의 함유량인 것으로 알려져 있다.When a child has a lot of collagen (collagen) in the body, the whole body is smooth, but with age, the collagen is insufficient or the quality is reduced, so the skin loses elasticity and wrinkles are formed. Wrinkles of the skin are affected by various factors such as skin moisture content, collagen content, and immune function, and it is known that collagen content is the most important.
콜라겐 분자는 파이브로블라스트(Fibroblast) 내에서 만들어지며, 이들이 세포 밖으로 분비된 후에 3중 나선 구조를 형성한다. 콜라겐의 주된 기능으로는, 피부의 기계적인 견고성 유지, 결합 조직의 저항력 및 조직의 결합력 강화, 세포 접착의 지탱, 세포 분할 및 분화의 유도 등이 알려져 있다(Van der Rest 등, 1990).Collagen molecules are made in fibroblasts, and after they are secreted out of cells, they form a triple helix structure. Main functions of collagen are known to maintain the mechanical rigidity of the skin, strengthen the resistance of connective tissues and tissues, sustain cell adhesion, induce cell division and differentiation (Van der Rest et al., 1990).
생체 내에서 콜라겐과 같은 세포외기질의 합성과 분해는 적절하게 조절되나 노화가 진행되면서 그 합성이 감소하며 콜라겐을 분해하는 효소인 기질 금속 단백질 분해효소(MMP, matrix metalloproteinase)의 발현이 촉진되어 피부의 탄력이 저하되고 주름이 형성된다. In vivo, the synthesis and degradation of extracellular matrix such as collagen is appropriately regulated, but the synthesis decreases as aging progresses, and the expression of matrix metalloproteinase (MMP), an enzyme that breaks down collagen, is promoted to promote Elasticity is reduced and wrinkles are formed.
또한, 자연 노화에 의한 세포 활성의 감소 및 콜라게나아제(collagenase)의 활성으로 인한 내적 요인 및 공해, 자외선, 스트레스 등의 외적 요인에 의한 활성산소 및 자유 라디칼의 증가로 인해 피부의 탄력이 감소하게 된다(Ingrid Emerit 등, Free radical and aging, 1992).In addition, the elasticity of the skin decreases due to an increase in free radicals and free radicals due to internal factors and pollution, ultraviolet rays, stress, etc., due to a decrease in cell activity due to natural aging and activity of collagenase. (Ingrid Emerit et al., Free radical and aging, 1992).
기질금속 단백질 분해효소(MMP)는 자연 노화와 자외선 노출에 의해서 생체 내에서 분비되는 단백질 분해제로 세포외기질(ECM, Extra Cellular Matrix)내의 결합조직(Collagen, Elastin, Glycosamino-glycans 등)을 분해하는 효소(Enzyme) 이다. Substrate metal protease (MMP) is a proteolytic agent secreted in vivo by natural aging and exposure to ultraviolet rays, which degrades connective tissue (Collagen, Elastin, Glycosamino-glycans) in the extracellular matrix (ECM). Enzyme.
기질금속 단백질 분해효소(MMP)는 다형 핵성 호중구 (Polymorphonuclearneutrophil), 대식 세포(Macrophage), 섬유아세포(Fibroblast), 골세포(Born cell)와 같은 세포로부터 분비되는 칼슘 및 아연의존성 엔도펩티다제(Endopeptidase)로 중성 pH에서 작용하며 기질로써 여러가지 세포외기질을 이용한다. 그 종류는 MMP 1~16까지 존재한다. 이중 collagen 분해와 관련하여 집중적으로 연구되는 기질금속 단백질 분해효소(MMP)는 MMP 1, MMP 9이다.Substrate metal protease (MMP) is a calcium and zinc-dependent endopeptidase (Endopeptidase) secreted from cells such as polymorphonuclear neutrophils, macrophages, fibroblasts, and bone cells. ) Acts at neutral pH and uses various extracellular substrates as substrates. Its types exist from MMP 1 to 16. Among these, matrix metal protease (MMP), which is intensively studied in relation to collagen degradation, is MMP 1 and MMP 9.
일반적으로 80세에는 20세에 비해 65%의 콜라겐이 감소하여 피부의 두께가 얇아지고, 이는 피부의 주름 형성과 밀접한 관련이 있다고 알려져 있다(Arthur K. Balin 등, Aging and the skin, 1989). In general, it is known that at 80 years of age, 65% of collagen is reduced compared to 20 years of age, and the thickness of the skin becomes thinner, which is closely related to the formation of wrinkles on the skin (Arthur K. Balin et al., Aging and the skin, 1989).
또한, 근래에는 피부 노화에 대한 광범위한 연구가 진행되면서 피부에서 콜라겐의 중요성이 한층 강조되고 있다. 피부 내 콜라겐의 합성촉진, MMP 1, MMP 9 등과 같은 기질 금속 단백질 분해효소의 활성억제를 통하여 진피 기질의 성분이 증가됨으로 이로부터 항산화, 주름개선, 탄력증진, 피부강화 및 상처 치유와 같은 항노화의 효과를 기대할 수 있는 것으로 알려져 있다. In addition, the importance of collagen in the skin has been further emphasized in recent years with extensive research on skin aging. Anti-aging such as anti-oxidation, wrinkle improvement, elasticity enhancement, skin strengthening and wound healing by increasing the composition of dermal matrix through the promotion of collagen synthesis in the skin and the inhibition of the activity of matrix metal protease such as MMP 1 and MMP 9 It is known that the effect of can be expected.
이러한 효과를 얻기 위하여, 종래에는 콜라겐을 화장품에 배합하여 제품화 하였다. 그러나 이들 화장품은 콜라겐을 피부 표면에 도포하는 것으로서, 고분자인 콜라겐의 경피 흡수가 어려워 그 기능을 기대할 수 없다. In order to obtain such an effect, conventionally, collagen was formulated into a cosmetic product. However, these cosmetics apply collagen to the surface of the skin, and it is difficult to expect its function due to the difficulty in percutaneous absorption of collagen, which is a polymer.
또한, 근래에는 피부의 진피에 직접 콜라겐을 주입하지만, 이 방법 역시 부작용으로 인해 올바른 피부 주름의 개선을 기대하기는 어렵다. In addition, although collagen is directly injected into the dermis of the skin in recent years, it is difficult to expect correct skin wrinkle improvement due to side effects.
최근에는 이 문제를 해결하고자 콜라겐 합성을 촉진하는 물질에 대한 관심이 높아졌으며, 콜라겐 합성 촉진물질로는 레티노익 산(Retinoic acid), 동물 태반 유래의 단백질(일본특허 특개평 8-231370)등이 알려졌다. In order to solve this problem, interest in substances that promote collagen synthesis has increased in recent years. Retinoic acid and protein derived from animal placenta (Japanese Patent Publication No. 8-231370) include collagen synthesis promoting substances. Became known.
그러나, 레티노익 산은 불안정하여 제형 기술이 복잡하며, 피부에 자극과 발적 등의 안전성 문제로 인해 사용에 한계가 있고, 동물 태반 유래의 단백질은 광우병에 의한 소 적출물의 사용이 금지되고 있으므로 실질적인 피부 콜라겐의 합성 촉진에 의한 피부 주름 개선효과를 기대하기는 어렵다. However, since retinoic acid is unstable, its formulation technology is complicated, and its use is limited due to safety problems such as irritation and redness to the skin, and protein derived from animal placenta is prohibited because the use of bovine extract due to mad cow disease is practical. It is difficult to expect the effect of improving skin wrinkles by promoting the synthesis of.
따라서, 생체에 안전하고 사용의 간편성이 있으며 또한 제형상 안정성이 높고 우수하며, 콜라겐 생성 촉진효과를 갖는 새로운 콜라겐 생성 촉진, MMP 1, MMP 9 등의 활성을 억제하는 물질의 개발과 이 물질을 함유하는 사용이 용이한 화장료 개발이 절실히 요구되고 있다.Therefore, it is safe and easy to use in the living body, and has high formulation stability and excellent, and the development of new collagen production promoting substances with collagen production promoting effect, MMP 1, MMP 9, etc. The development of an easy-to-use cosmetic is urgently required.
한편, 새싹은 발아하고 본 잎이 나오기 전의 상태를 의미하는 것으로서, 복숭아 새싹 추출물은 복숭아의 싹이 튼 어린식물에서 추출한 물질을 의미한다. 새싹은 식물의 줄기세포라고 할 수 있다. 식물은 동물과 달리 식물체 전체가줄기세포가 될 수 있는 가능성을 가지고 있는데 이는 식물체가 조직만 가지고 있고 기관으로까지 분화되어 있지 않기 때문이다. 식물의 대표적인 성체줄기세포는 뿌리나 줄기 끝의 생장점에 있는 세포이고, 씨앗 등은 동물의 배아줄기세포에 대응된다고 할 수 있다. 새싹에는 성장에 필요한 비타민, 단백질, 아미노산, 무기질 등의 다양한 물질들이 풍부하게 포함되어 있다. On the other hand, the sprout refers to the state before germination and the leaves appear, and the peach sprout extract refers to the material extracted from the young plants with sprouted peaches. A bud is a stem cell of a plant. Unlike animals, plants have the potential to become whole stem cells because plants have only tissue and are not differentiated into organs. It can be said that typical adult stem cells of plants are cells at the growth point of the root or the end of the stem, and seeds and the like correspond to the embryonic stem cells of animals. The sprouts are rich in various substances such as vitamins, proteins, amino acids, and minerals necessary for growth.
그리고 수수(Sorglum Bicolor)는 외떡잎식물 벼목 화본과의 한해살이풀로, 내건성이 강해 척박한 땅이나 건조한 땅에서도 자생하여 아시아 및 아프리카 전역에서 중요한 식량원으로 재배되고 있으며, 우리나라에서도 쌀, 보리, 밀, 옥수수와 함께 식량자원으로 재배되고 있다. 수수에는 다양한 품종이 존재하고, 용도에 따라 곡용수수(grain sorghum), 단수수(sorgo), 장목수수(broom-corn)로 분류된다(Chang et al., 2005). 장목수수는 이삭에 마디가 없고 꼬투리에 달린 줄기의 길이는 20~30cm이며, 열매는 두꺼운 껍질이 탈곡을 해도 벗겨지지 않기 때문에 종자로만 이용된다. 민간요법에서는 수수를 식욕개선, 소화촉진, 체온유지, 위장보호, 해독 등의 다양한 증상에 활용해왔다. 수수에는 식이섬유, phenolic compounds, tannins 등의 다양한 유효 성분이 함유되어있다고 알려져 있으나, 붉은수수는 tannin이 함유되어 있지 않는 특징이 있다. 수수에 함유된 phenolic compounds는 flavonoids, phenolic acids, antocyanins 등으로 구성되어 있으며, 대부분이 flavonoids로 알려져 있다(Ryu HS, Kim J, Kim HS. 2006. Enhancing effect of sorghum bicolor L. Moench (sorghum, su su) extracts on mouse spleen and macrophage cell activation. J. Korean Diet. Assoc. 19: 176-182.) (Chae KY, Hong JS. 2006. Quality characteristics of Sulgidduk with different amounts of waxy sorghum flour. Korean J Food Cookery Sci 22: 363-369., Chang HG, Park YS. 2005. Effects of waxy and normal sorghum flours on sponge cake properties. Food Eng Prog 9: 199-207.) (Kim KO, Kim HS, Ryu HS. 2006. Effect of Sorghum bicolor L. Moench(sorghum, su-su) Water extracts on mouse immune cell activation. J 43-13 2017-03-31 Korean Diet Assoc 12: 82-88.).And Sorglum Bicolor is a perennial plant with a monocotyledonous plant, which is strong and resistant to growing in rugged or arid lands, and is cultivated as an important food source across Asia and Africa.In Korea, rice, barley, wheat, and corn It is grown as a food resource. A variety of varieties exist in sorghum, and are classified into grain sorghum, sorgo, and broom-corn according to their use (Chang et al., 2005). Jangmoksu has no nodes on the ears, and the length of the stem attached to the pod is 20~30cm, and the fruit is used only as a seed because it does not peel off even if the thick skin is threshed. Folk remedies have used sorghum for a variety of symptoms such as improving appetite, promoting digestion, maintaining body temperature, protecting the stomach, and detoxifying. Sorghum is known to contain various active ingredients such as dietary fiber, phenolic compounds, and tannins, but red sorghum has a characteristic that it does not contain tannins. The phenolic compounds contained in sorghum consist of flavonoids, phenolic acids, antocyanins, etc., most of which are known as flavonoids (Ryu HS, Kim J, Kim HS. 2006.Enhancing effect of sorghum bicolor L. Moench (sorghum, su su su ) extracts on mouse spleen and macrophage cell activation.J. Korean Diet.Assoc. 19: 176-182.) (Chae KY, Hong JS. 2006.Quality characteristics of Sulgidduk with different amounts of waxy sorghum flour.Korean J Food Cookery Sci 22: 363-369., Chang HG, Park YS.2005.Effects of waxy and normal sorghum flours on sponge cake properties.Food Eng Prog 9: 199-207.) (Kim KO, Kim HS, Ryu HS. 2006.Effect of Sorghum bicolor L. Moench (sorghum, su-su) Water extracts on mouse immune cell activation.J 43-13 2017-03-31 Korean Diet Assoc 12: 82-88.).
이에 본 발명자는 피부 주름을 개선하는 효과와 유효성을 극대화시키기 위한 발효기술을 기초로 하여 연구한 결과, 수수새싹 추출액을 사카로미세스로 발효한 발효액 또는 이의 발효 추출액이 현저하게 분자내 Collagen, TIMP-1, Tropoelastin의 유전자 발현과 MMP-1의 유전자 발현을 억제하여 피부 개선 효과를 나타냄을 확인하여 본 발명을 완성하였다.Accordingly, the present inventors researched on the basis of fermentation technology for maximizing the effectiveness and effectiveness of improving skin wrinkles, and the fermentation broth fermented by Saccharomyces or the fermentation extract thereof was significantly intramolecular Collagen, TIMP- 1, by inhibiting the gene expression of Tropoelastin and the gene expression of MMP-1 was confirmed to show the effect of improving the skin was completed the present invention.
본 발명은 수수새싹 추출액을 사카로미세스로 발효한 발효액 또는 상기 발효액을 추출한 발효 추출액을 수득하여 피부 주름개선 효과가 우수하고 피부 자극에 대한 부작용이 없는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물 및 그의 제조방법을 제공하는데 있다.The present invention obtains a fermentation broth obtained by fermenting the sorghum sprout extract with Saccharomyces or a fermentation extract obtained by extracting the fermentation solution, and has an excellent effect on improving skin wrinkles and has no side effects on skin irritation. It is to provide a cosmetic composition for improving skin wrinkles and a method for manufacturing the same.
또한, 본 발명은 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 화장료 조성물을 제조함으로써, 섬유아세포 증식 촉진, 분자내 Collagen, TIMP-1, Tropoelastin의 유전자 발현과 MMP-1의 유전자 발현을 억제하여 피부의 주름을 개선하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물 및 그의 제조방법을 제공하는데 있다.In addition, the present invention promotes fibroblast proliferation, intramolecular collagen, TIMP-1, tropoelastin gene expression and gene expression of MMP-1 by preparing a fermentation broth of a sorghum sprout extract or a cosmetic composition containing the fermentation extract thereof as an active ingredient. It is to provide a cosmetic composition for improving skin wrinkles and a method for manufacturing the same, using the fermentation broth of the sorghum sprout extract to improve the wrinkles of the skin by inhibiting or the fermentation extract thereof as an active ingredient.
본 발명이 해결하고자 하는 다양한 과제들은 이상에서 언급한 과제들에 제한되지 않으며, 언급되지 않은 또 다른 과제들은 아래의 기재로부터 당업자에게 명확하게 이해될 수 있을 것이다.Various problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.
본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물은 수수새싹 추출액을 사카로미세스로 발효시켜 수득한 수수새싹 발효액 또는 이의 발효 추출액을 함유한다.The cosmetic composition for improving skin wrinkles containing the fermentation broth of the sorghum sprout extract according to the present invention or the fermentation extract thereof as an active ingredient contains the sorghum sprout fermentation broth obtained by fermenting the sorghum sprout extract with Saccharomyces or its fermentation extract.
상기 수수새싹은 물, 탄소수 1 내지 4개의 무수 또는 함수 저급 알코올, 아세톤, 에틸아세테이트, 부틸아세테이트 및 1,3-부틸렌 글리콜로 구성된 군으로부터 선택되는 어느 하나의 추출 용매를 사용하여 추출될 수 있다.The sorghum sprout can be extracted using any extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol. .
상기 수수새싹 추출액의 발효액 또는 이의 발효 추출액은 화장료 조성물 총 중량에 대해 0.05 내지 20.0 중량%로 포함될 수 있다.The fermentation broth of the sorghum sprout extract or the fermentation extract thereof may be included in an amount of 0.05 to 20.0 wt% based on the total weight of the cosmetic composition.
상기 수수새싹 추출액의 발효액 또는 이의 발효 추출액은 피부 주름 개선 효과를 가지며 피부 자극에 대한 부작용이 없다.The fermentation broth of the sorghum sprout extract or the fermentation extract thereof has an effect of improving skin wrinkles and has no side effects on skin irritation.
또한, 본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물의 제조방법은 수수새싹 추출액을 얻는 단계(S10); 상기 수수새싹 추출액에 균주 사카로미세스를 접종하여 발효액을 얻는 단계(S20); 상기 단계(S20)의 수수새싹 발효액에서 배양균을 제거한 후 중탕 추출법을 이용하여 수수새싹 발효 추출액을 얻는 단계(S30); 및 상기 단계(S20)의 수수새싹 발효액 또는 상기 단계(S30)의 수수새싹 발효 추출액에 안정화제, 비타민, 안료, 색소 및 향료를 더 포함하여 화장료 조성물을 얻는 단계(S40)를 포함한다.In addition, the method for preparing a cosmetic composition for improving skin wrinkles using the fermentation broth of the sorghum sprout extract according to the present invention or the fermentation extract thereof as an active ingredient comprises: obtaining a sorghum sprout extract (S10); Inoculating the strain Saccharomyces in the sorghum sprout extract to obtain a fermentation broth (S20); Removing the cultured bacteria from the sorghum sprout fermentation broth of the step (S20) and obtaining a sorghum sprout fermentation extract using a hot water extraction method (S30); And obtaining a cosmetic composition (S40) further comprising a stabilizer, a vitamin, a pigment, a pigment, and a fragrance in the sorghum sprout fermentation liquid of the step (S20) or the sorghum sprout fermentation extract of the step (S30).
상기 단계(S20)에서 수득한 수수새싹 발효액 또는 단계(S30)에서 수득한 수수새싹 발효 추출액은 화장료 조성물 총 중량에 대해 0.05 내지 20.0중량%로 포함될 수 있다.The sorghum sprout fermentation broth obtained in step S20 or the sorghum sprout fermentation extract obtained in step S30 may be included in an amount of 0.05 to 20.0 wt% based on the total weight of the cosmetic composition.
상기 단계(S10)는 상기 수수새싹을 정제수로 세척하고 건조한 다음 2cm 이내로 세절하여 세절된 시료를 얻으며, 상기 수수새싹 세절물에 50 내지 100g/L의 증류수를 가하고 중탕 조건에서 3 내지 8시간씩 3회 환류추출하고 냉침을 한 후, 3 내지 8㎛ 여과지로 여과하여 추출액을 얻을 수 있다.In the step (S10), the sorghum bud is washed with purified water, dried, and then shredded to within 2 cm to obtain a shredded sample. 50 to 100 g/L of distilled water is added to the sorghum bud shredded water, and 3 to 8 hours for 3 to 8 hours under hot water condition. After reflux extraction and cold immersion, the extract can be obtained by filtration with 3 to 8 μm filter paper.
상기 단계(S20)는 상기 단계(S10)에서 얻은 수수새싹 추출액에 균주 사카로미세스와 YPD Broth 배지를 넣어 25 내지 35℃ 호기적 조건에서 48 내지 72시간 발효를 진행하며, 배양 후 더 이상 배양되지 않도록 멸균(121℃, 15분, 1.5기압)한 후 수수새싹 발효물에 30%(V/V) 알코올을 첨가하여 5시간씩 3회 환류중탕추출하고 냉침하고, 냉침한 후 원심분리하여 배양균을 1차 제거하여 얻은 여과액을 다시 여과기를 이용하여 0.2㎛까지 여과한 후 멸균 처리를 할 수 있다.In the step (S20), strain Saccharomyces and YPD Broth medium is added to the sorghum sprout extract obtained in the step (S10) to perform fermentation for 48 to 72 hours at 25 to 35°C aerobic conditions, and no further culture after cultivation After sterilization (121℃, 15 minutes, 1.5 atm), 30% (V/V) alcohol was added to the fermented sorghum sprouts, extracted under reflux three times for 5 hours, and then cold-cooled. The filtrate obtained by primary removal may be filtered again to 0.2 μm using a filter, and then sterilized.
상기 단계(S30)는 상기 단계(S20)에서 수득한 수수새싹 발효액을 30%(V/V) 알코올을 첨가하여 3 내지 6시간씩 3회 환류중탕 추출하고 냉침을 한 후 멸균 및 방부제를 투여 및 원심분리와 3 내지 8㎛ 여과지로 여과하여 상기 배양균을 제거할 수 있다.In the step (S30), 30% (V/V) alcohol is added to the sorghum sprout fermentation liquid obtained in the step (S20) to extract the reflux water 3 times for 3 to 6 hours, sterilize and preservative after cold immersion, and The cultured bacteria can be removed by centrifugation and filtration with 3 to 8 μm filter paper.
기타 실시 예들의 구체적인 사항들은 상세한 설명에 포함되어 있다Details of other embodiments are included in the detailed description
본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물은 수수새싹 추출액을 사카로미세스로 발효한 발효액 또는 상기 발효액을 추출한 발효 추출액을 수득하여 피부 주름개선 효과가 우수하고 피부 자극에 대한 부작용이 없다.The cosmetic composition for improving skin wrinkles containing the fermentation broth of the sorghum sprout extract according to the present invention or the fermentation extract thereof as an active ingredient, improves skin wrinkles by obtaining a fermentation broth fermented with a sorghum sprout extract with Saccharomyces or a fermentation extract obtained by extracting the fermentation broth Excellent effect and no side effects to skin irritation.
또한, 본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물의 제조방법은 수수새싹 추출액의 발효액을 유효성분으로 함유하는 화장료 조성물을 제조함으로써, 섬유아세포 증식 촉진, 분자내 Collagen, TIMP-1, Tropoelastin의 유전자 발현과 MMP-1의 유전자 발현을 억제하여 피부 주름을 개선할 수 있다.In addition, the method for preparing a cosmetic composition for improving skin wrinkles containing a fermentation broth of a sorghum sprout extract or a fermentation extract thereof as an active ingredient, by preparing a cosmetic composition containing a fermentation broth of a sorghum sprout extract as an active ingredient, fibroblasts Skin wrinkles can be improved by promoting proliferation, inhibiting intramolecular collagen, TIMP-1, and Tropoelastin gene expression and MMP-1 gene expression.
본 발명의 기술적 사상의 실시예는, 구체적으로 언급되지 않은 다양한 효과를 제공할 수 있다는 것이 충분히 이해될 수 있을 것이다.It will be fully understood that embodiments of the technical idea of the present invention can provide various effects not specifically mentioned.
도 1은 본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물의 제조방법을 설명하기 위한 순서도이다.1 is a flow chart for explaining a method of manufacturing a cosmetic composition for improving skin wrinkles containing a fermentation broth of a sorghum sprout extract according to the present invention or a fermentation extract thereof as an active ingredient.
본 발명의 이점 및 특징, 그리고 그것들을 달성하는 방법은 상세하게 후술되어 있는 실시예를 참조하면 명확해질 것이다. 그러나 본 발명은 여기서 설명되는 실시예들에 한정되지 않고 다른 형태로 구체화될 수도 있다. 오히려, 여기서 소개되는 실시예들은 개시된 내용이 철저하고 완전해질 수 있도록 그리고 당업자에게 본 발명의 사상이 충분히 전달될 수 있도록 하기 위해 제공되는 것이다.Advantages and features of the present invention, and methods for achieving them will be clarified with reference to embodiments described below in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided to ensure that the disclosed contents are thorough and complete and that the spirit of the present invention is sufficiently conveyed to those skilled in the art.
본 출원에서 사용한 용어는 단지 특정한 실시예를 설명하기 위해 사용된 것으로, 본 발명을 한정하려는 의도가 아니다. 단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한, 복수의 표현을 포함한다.The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise.
다르게 정의되지 않는 한, 기술적이거나 과학적인 용어를 포함해서 여기서 사용되는 모든 용어들은 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미가 있다. 일반적으로 사용되는 사전에 정의되어 있는 것과 같은 용어들은 관련 기술의 문맥상 가지는 의미와 일치하는 의미가 있는 것으로 해석되어야 하며, 본 출원에서 명백하게 정의하지 않는 한, 이상적이거나 과도하게 형식적인 의미로 해석되지 않는다.Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person skilled in the art to which the present invention pertains. Terms such as those defined in a commonly used dictionary should be interpreted as having meanings consistent with meanings in the context of related technologies, and should not be interpreted as ideal or excessively formal meanings unless explicitly defined in the present application. Does not.
이하, 본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물에 대하여 더욱 상세하게 설명하기로 한다.Hereinafter, a cosmetic composition for improving skin wrinkles containing the fermentation broth of the sorghum sprout extract according to the present invention or the fermentation extract thereof as an active ingredient will be described in more detail.
본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물은 수수새싹 추출액을 사카로미세스로 발효시켜 수득한 수수새싹 추출액의 발효액을 함유하는 피부 주름개선용 화장료 조성물인 것을 특징으로 한다.The cosmetic composition for improving skin wrinkles containing the fermentation broth of the sorghum sprout extract according to the present invention or the fermentation extract thereof as an active ingredient is for improving skin wrinkles containing the fermentation broth of the sorghum sprout extract obtained by fermenting the sorghum sprout extract with Saccharomyces It is characterized by being a cosmetic composition.
또한, 본 발명은 수수새싹 추출액을 사카로미세스로 발효시켜 수득한 수수새싹 발효액으로부터 추출한 발효 추출액을 함유하는 피부 주름개선용 화장료 조성물인 것을 특징으로 한다.In addition, the present invention is characterized in that the cosmetic composition for improving skin wrinkles containing a fermentation extract extracted from a sorghum sprout fermentation broth obtained by fermenting a sorghum sprout extract with Saccharomyces.
종래 기술에서는 수수 잎/줄기 추출물 또는 발효물, 수수알곡 추출물 또는 발효물, 기타 식물의 새싹 추출물 및 새싹의 발효 추출물을 화장료 조성물로 사용하고 있었으나, 본 발명에서는 피부주름 개선 효과와 유효성을 극대화시키기 위해서 수수새싹만을 사용하였으며, 또한 유효성분의 피부흡수 효율을 높여 피부주름 개선 효과를 높이기 위해 수수새싹 추출액을 사카로미세스로 발효시킨 발효액 또는 이의 발효 추출액을 피부주름 개선용 화장료 조성물로 사용하여 분자내 Collagen, TIMP-1, Tropoelastin의 유전자 발현과 MMP-1의 유전자 발현을 종래보다 현저하게 억제하여 피부 개선 효과를 극대화시켰다.In the prior art, sorghum leaf/stem extract or fermented product, sorghum grain extract or fermented product, other plant sprout extract and bud ferment extract were used as cosmetic compositions, but in the present invention, in order to maximize the effectiveness and effectiveness of improving skin wrinkles Only sorghum sprout was used, and in order to increase the skin absorption efficiency of the active ingredient and improve the effect of improving skin wrinkles, the fermentation broth fermented with sorghum sprout extract or Saccharomyces or the fermentation extract thereof is used as a cosmetic composition for improving skin wrinkles. , TIMP-1, Tropoelastin gene expression and MMP-1 gene expression were significantly suppressed than before to maximize the skin improvement effect.
상기 수수새싹의 바람직한 건조 중량비는 처음 수수새싹 중량의 5 내지 30 중량%가 되는 것이 바람직하며, 상기 수수새싹은 물, 탄소수 1 내지 4개의 무수 또는 함수 저급 알코올, 아세톤, 에틸아세테이트, 부틸아세테이트 및 1,3-부틸렌 글리콜로 이루어진 군에서 선택된 어느 하나 이상의 추출 용매를 사용하여 추출되는 것이 바람직하다.The preferred dry weight ratio of the sorghum sprout is preferably 5 to 30% by weight of the first sorghum sprout, and the sorghum sprout is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1 It is preferably extracted using any one or more extraction solvents selected from the group consisting of ,3-butylene glycol.
상기 수수새싹은 당업계에 공지된 통상의 방법에 따라, 즉, 통상적인 온도와 압력의 조건하에서, 통상적인 용매를 사용하여 수수새싹 추출액을 제조될 수 있다. The sorghum sprout can be prepared according to conventional methods known in the art, that is, under conditions of conventional temperature and pressure, using a conventional solvent.
예를 들어, 본 발명의 조성물의 유효성분인 수수새싹 추출액은 물, 탄소수 1 내지 4개의 무수 또는 함수 저급 알코올, 아세톤, 에틸아세테이트, 부틸아세테이트 및 1,3-부틸렌 글리콜로 이루어진 군에서 선택된 어느 하나 이상의 용매를 사용하여 추출하거나 수수새싹을 상기 용매를 이용하여 추출한 후, 이를 혼합하여 제조할 수 있다.For example, the sorghum sprout extract, which is an active ingredient of the composition of the present invention, is selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol. After extraction using one or more solvents or extracting sorghum buds using the solvent, it can be prepared by mixing it.
본 발명에서 상기 용매는 알코올이 사용될 수 있더, 바람직하게는 에탄올이 사용될 수 있는데, 상기 에탄올은 더욱 바람직하게 30 ~ 50중량% 에탄올인 것이 바람직하다. 또한, 상기 추출 용매의 적합한 첨가량은 수수새싹 건조 중량의 5 내지 30배 중량이며, 더욱 바람직하게는 10 내지 30배 중량이며, 가장 바람직하게는 20배 중량이다.In the present invention, the solvent may be used alcohol, preferably ethanol may be used, the ethanol is more preferably 30 to 50% by weight ethanol. In addition, a suitable addition amount of the extraction solvent is 5 to 30 times the weight of the sorghum sprout dry weight, more preferably 10 to 30 times the weight, and most preferably 20 times the weight.
한편, 본 발명의 수수새싹 추출액을 사카로미세스로 발효한 발효액은 상술한 추출법에 의한 추출물뿐만 아니라, 통상적인 정제과정을 거친 추출물도 포함한다. On the other hand, the fermentation broth fermented with Saccharomyces of the sorghum sprout extract of the present invention includes not only extracts by the above-described extraction method, but also extracts that have been subjected to a conventional purification process.
예를 들어, 일정한 분자량 컷-오프 값을 갖는 한외 여과막을 이용한 분리, 다양한 크로마토그래피(크기, 전하, 소수성 또는 친화성에 따른 분리를 위해 제작된 것)에 의한 분리 등, 추가적으로 실시된 다양한 정제 방법을 통해 얻어진 분획도 본 발명의 수수새싹 추출액을 사카로미세스로 발효한 발효액에 포함된다고 해석할 수 있다.For example, various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, and separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity) It can be interpreted that the fraction obtained through is also included in the fermentation broth fermented with Saccharomyces of the sorghum sprout extract of the present invention.
또한, 본 발명의 수수새싹 추출액을 사카로미세스로 발효한 발효액은 발효액을 감압 증류 및 동결 건조 또는 분무 건조 등과 같은 추가적인 과정에 의해 분말 상태로 제조된 것을 포함하기도 한다.In addition, the fermentation broth fermented with Saccharomyces of the sorghum sprout extract of the present invention may include those prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.
본 발명의 수수새싹 추출액의 발효액 또는 수수새싹 발효 추출액은 상기 수수새싹 추출액을 사카로미세스로 발효한 발효액 또는 상기 발효액을 추출한 발효 추출액으로써, 섬유아세포 증식 촉진, 분자내 Collagen, TIMP-1, Tropoelastin의 유전자 발현과 MMP-1의 유전자 발현을 억제하여 피부의 주름개선 효과가 우수하고 피부 자극에 대한 부작용이 없는 천연의 안전 제제에 해당하며, 생산비용이 저렴하다. The fermentation broth or sorghum sprout fermentation extract of the sorghum sprout extract of the present invention is a fermentation broth fermented with the saccharomyces extract or a fermentation extract obtained by extracting the fermentation broth, promoting fibroblast proliferation, intramolecular collagen, TIMP-1, Tropoelastin By suppressing gene expression and gene expression of MMP-1, it is excellent in wrinkle improvement effect of skin and is a natural safety preparation without side effects on skin irritation, and has low production cost.
본 발명에서 수수새싹 추출액을 사카로미세스로 발효한 발효액은 농축된 액상일 수도 있고, 건조된 분말형태일 수도 있다.In the present invention, the fermentation broth fermented from Sorghum bud extract with Saccharomyces may be a concentrated liquid or a dried powder.
상기 발효단계의 균주는 자낭균류에 속하는 1속으로, 난형 또는 타원형이며 다극 출아로 증식하는 유포자 효모로, 상기 사카로미세스는 사카로미세스 바이아누스(Saccharomyces bayanus), 사카로미세스 바울라디(Saccharomyces boulardii), 사카로미세스 세레비시아(Saccharomyces cerevisiae), 사카로미세스 케발리에리(Saccharomyces chevalieri), 사카로미세스 다이레넨시스(Saccharomyces dairenensis), 사카로미세스 엘립소이두스(Saccharomyces ellipsoideus), 사카로미세스 유바야누스(Saccharomyces eubayanus), 사카로미세스 엑시구스(Saccharomyces exiguus), 사카로미세스 플로렌시노(Saccharomyces florentinus) 및 사카로미세스 우바룸(Saccharomyces uvarum)으로 이루어진 군에서 선택된 어느 하나 이상의 균주일 수 있다.The strain of the fermentation step is a first genus belonging to the ascites fungus, an ovate or ellipsoid, a diffuse yeast that multiplies with multipolar germination, and Saccharomyces is Saccharomyces bayanus , Saccharomyces boulardii ), Saccharomyces cerevisiae , Saccharomyces chevalieri , Saccharomyces dairenensis , Saccharomyces elirenessis , Saccharomyces ellipsoideus , Saccharomyces ellipsoideus may be a yuba Janus (Saccharomyces eubayanus), saccharose in MRS eksi Goose (Saccharomyces exiguus), saccharose in MRS Florencio Sino (Saccharomyces florentinus) and a saccharide as a MRS Uva room (Saccharomyces uvarum) any one or more strains selected from the group consisting of.
본 발명의 수수새싹 추출액의 발효액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물은 우수한 주름 개선 효능을 나타내며, 적용 제품들에서 우수한 안정성을 나타낸다. The cosmetic composition for improving skin wrinkles, which contains the fermentation broth of the sorghum sprout extract of the present invention as an active ingredient, exhibits excellent wrinkle improvement efficacy and excellent stability in applied products.
또한, 섬유아세포 증식 촉진, 분자내 Collagen, TIMP-1, Tropoelastin의 유전자 발현과 MMP-1의 유전자 발현을 억제하여 피부의 주름을 개선하는 효과를 가진다.In addition, it promotes fibroblast proliferation, inhibits intramolecular Collagen, TIMP-1, and Tropoelastin gene expression and MMP-1 gene expression, thereby improving skin wrinkles.
본 발명의 바람직한 실시예에 따르면, 수수새싹 추출액을 사카로미세스로 발효한 발효액 또는 발효액을 추출한 발효 추출액은 화장료 조성물 중 총 중량대비 0.05 내지 20.0 중량%이며, 바람직하게는 0.5 내지 15.0 중량%이며, 보다 바람직하게는 1 내지 10.0 중량%이다. According to a preferred embodiment of the present invention, the fermentation broth or the fermentation extract extracting the fermentation broth fermented with Sorghum sprout extract is 0.05 to 20.0 wt% of the total weight of the cosmetic composition, preferably 0.5 to 15.0 wt%, More preferably, it is 1 to 10.0 weight%.
상기 수수새싹 추출액의 발효액 또는 발효액을 추출한 발효 추출액은 화장료 조성물 총 중량대비 0.05 중량% 미만인 경우 섬유아세포 증식 촉진, 분자내 Collagen, TIMP-1, Tropoelastin의 유전자 발현과 MMP-1의 유전자 발현에 대한 억제효과가 나타나지 않는 문제가 있으며, 20 중량% 초과인 경우 건조 피부를 가진 사람에게 피부 가려움과 따끔거리는 문제가 발생할 수 있다. The fermentation broth of the sorghum sprout extract or the fermentation extract obtained by extracting the fermentation broth promotes fibroblast proliferation when it is less than 0.05 wt% of the total weight of the cosmetic composition, and suppresses the gene expression of Collagen, TIMP-1, Tropoelastin in the molecule and gene expression of MMP-1. There is a problem that the effect does not appear, and when it is more than 20% by weight, itching and tingling of the skin may occur in people with dry skin.
본 발명의 수수새싹 추출액의 발효액 또는 발효액을 추출한 발효 추출액은 피부 주름 개선의 효과를 가지며, 건조 피부를 가진 사람을 제외하고는 피부 자극에 대한 부작용이 없기 때문에, 20중량%를 초과하여 100 중량%까지 수수새싹 추출액의 발효액 또는 발효액을 추출한 발효 추출액을 화장료 조성물로 사용할 수 있다. The fermentation broth of the sorghum sprout extract of the present invention or the fermentation extract from which the fermentation broth is extracted has an effect of improving skin wrinkles and has no side effects on skin irritation except for people with dry skin, so it exceeds 100% by weight in excess of 20% by weight Until the fermentation broth of the sorghum sprout extract or the fermentation extract from which the fermentation broth was extracted can be used as a cosmetic composition.
본 발명의 화장료 조성물에 포함되는 성분은 유효 성분으로서의 수수새싹 추출액을 사카로미세스로 발효한 발효액 이외에 화장료 조성물에 통상적으로 이용되는 성분들을 포함하며, 예컨대 항산화제, 안정화제, 용해화제, 비타민, 안료, 색소 및 향료와 같은 통상적인 보조제, 그리고 담체를 포함한다.The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions other than the fermentation broth fermented with Saccharomyces as an active ingredient, such as antioxidants, stabilizers, solubilizers, vitamins, pigments , Conventional adjuvants such as pigments and flavors, and carriers.
본 발명의 화장료 조성물은 당 업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다. 보다 상세하게는, 유연 화장수, 영양 화장수, 영양 크림, 맛사지 크림, 에센스, 팩, 아이 크림, 클렌징 크림, 클렌징 포옴, 클렌징 워터, 스프레이 또는 파우더의 제형으로 제조될 수 있다.The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleaning , May be formulated as an oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, pack, eye cream, cleansing cream, cleansing foam, cleansing water, spray or powder.
본 발명의 화장료 조성물은 당업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예들 들어 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 스프레이 등으로 제형화될 수 있으나, 이에 한정되지는 않는다. 보다 상세하게는 스킨, 로션, 유연 화장수, 영양 화장수, 영양 크림, 마사지 크림, 에센스, 아이 크림, 클렌징크림, 클렌징폼, 클렌징워터, 팩, 스프레이 또는 파우더의 제형으로 제조될 수 있다.The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, oils, powder foundations, emulsions It may be formulated as a foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it can be prepared in the form of skin, lotion, soft lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성유, 식물성유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 중 어느 하나 이상을 선택하여 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, any one or more of animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as carrier components It can be used by selecting.
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally chlorofluorohydrocarbon, propane /Propellant such as butane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 부틸렌글리콜, 1,3-부틸글리콜 오일, 폴리옥시에칠렌 경화피마자유, 글리세롤, 글리세린, 지방족 에스테르, 페녹시에탄올, 트리에탄올아민, 폴리에틸렌 글리콜, 밀납, 폴리솔베이트 60, 솔비탄세스퀴오레이드, 파라핀, 소르비탄 스테아레이트, 친유형 모노스테아린산 글리세린, 스테아린산, 글리세릴스테아레이트/피이지-400 스테아레이트, 카르복시폴리머, 시토스테롤, 폴리글리세릴 2-올레이트, 세라마이드, 콜레스테롤, 스테아레스-4, 디세틸포스페이트, 마카다미아 오일, 카르복시비닐폴리머, 산탄검 및 소르비탄의 지방산 에스테르로 이루어진 군에서 선택된 어느 하나 이상이 이용될 수 있다.When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butyl Ren glycol, 1,3-butyl glycol oil, polyoxyethylene cured castor oil, glycerol, glycerin, aliphatic esters, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleide, paraffin, Sorbitan stearate, lipophilic monostearic acid glycerin, stearic acid, glyceryl stearate/page-400 stearate, carboxypolymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, steareth-4, disetyl phosphate , Macadamia oil, carboxyvinyl polymer, any one or more selected from the group consisting of fatty acid esters of xanthan gum and sorbitan can be used.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올, 글리세린, 부틸렌글리콜 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 하이드록시에틸셀룰로오즈, 소디움히아루로네이트, 페녹시에탄올, 알루미늄 메타히드록시드, 벤토나이트, 스테아린산, 세틸알코올, 글리세릴모노스테아레이트, 폴리옥시에틸렌소르비탄모노스테아레이트, 솔비탄세스퀴올레이트, 글리세릴모노스테아레이트 /글리세릴스테아레이트/폴리옥시에틸렌스테아레이트, 왁스, 파리핀, 스쿠알란, 카프릴릭 /카프릭트리글리세라이드, 카르복시비닐폴리머, 트리에탄올아민 및 아가 또는 트라칸트로 이루어진 군에서 선택된 어느 하나 이상이 이용될 수 있다.When the formulation of the present invention is a suspension, as a carrier component, liquid diluents such as water, ethanol, glycerin, butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Same suspending agent, microcrystalline cellulose, hydroxyethylcellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate , Sorbitan sesquioleate, glyceryl monostearate/glyceryl stearate/polyoxyethylene stearate, wax, parffin, squalane, caprylic/capric triglyceride, carboxyvinyl polymer, triethanolamine and agar or tra Any one or more selected from the group consisting of Kant may be used.
본 발명의 제형이 계면-활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 라놀린 유도체 및 에톡실화 글리세롤 지방산 에스테르로 이루어진 군에서 선택된 어느 하나 이상이 이용될 수 있다.When the formulation of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide as a carrier component Any one or more selected from the group consisting of ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative and ethoxylated glycerol fatty acid ester can be used.
이하, 첨부된 도면을 참조하여 본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물의 제조방법에 대하여 더욱 상세하게 설명하기로 한다.Hereinafter, with reference to the accompanying drawings will be described in more detail with respect to the manufacturing method of the cosmetic composition for improving wrinkles of the skin containing the fermentation broth of the sorghum sprout extract according to the present invention or a fermentation extract thereof as an active ingredient.
도 1은 본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물의 제조방법을 설명하기 위한 순서도이다.1 is a flow chart for explaining a method of manufacturing a cosmetic composition for improving skin wrinkles containing a fermentation broth of a sorghum sprout extract according to the present invention or a fermentation extract thereof as an active ingredient.
이하, 본 발명에 대한 바람직한 실시 내용은 첨부한 도면을 참조하여 설명하면, 본 발명의 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물의 제조방법은 하기 단계를 포함하는 것이 바람직하나 이에 한정되지 않는다.Hereinafter, a preferred embodiment of the present invention will be described with reference to the accompanying drawings, a method for preparing a cosmetic composition for improving skin wrinkles containing the fermentation broth of the sorghum sprout extract or the fermentation extract thereof as an active ingredient. It is preferred to include, but is not limited thereto.
도 1을 참조하면, 본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물의 제조방법은 수수새싹 추출액을 얻는 단계(S10), 상기 수수새싹 추출액에 균주 사카로미세스를 접종하여 발효액을 얻는 단계(S20), 상기 단계(S20)의 수수새싹 발효액을 중탕 추출법을 이용하여 수수새싹 발효액을 추출하고 배양균을 제거한 후 발효 추출액을 얻는 단계(S30) 및 상기 단계(S20)의 수수새싹 발효액 또는 상기 단계(S30)의 수수새싹 발효 추출액에 안정화제, 비타민, 안료, 색소 및 향료를 더 포함하여 화장료 조성물을 얻는 단계(S40)를 포함한다.Referring to Figure 1, the method of manufacturing a cosmetic composition for improving skin wrinkles containing a fermentation broth or extract of fermented extract according to the present invention as an active ingredient step (S10) to obtain a sorghum sprout extract, the sorghum sprout extract Obtaining a fermentation broth by inoculating the strain Saccharomyces (S20), extracting the sorghum sprout fermentation broth from the sorghum sprout fermentation broth of step (S20) and removing the culture bacteria to obtain a fermentation extract (S30) and It includes a step (S40) of obtaining a cosmetic composition further comprising a stabilizer, vitamins, pigments, pigments and flavors in the sorghum sprout fermentation liquid of the step (S20) or the sorghum sprout fermentation extract of the step (S30).
본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물의 제조방법을 각 단계별로 나누어서 설명하면 다음과 같다.The method for preparing a cosmetic composition for improving skin wrinkles containing the fermentation broth of the sorghum sprout extract according to the present invention or the fermentation extract thereof as an active ingredient will be described in detail as follows.
먼저, 상기 수수새싹 추출액을 얻는 단계(S10)를 수행한다.First, a step (S10) of obtaining the extract of sorghum sprout is performed.
상기 단계(S10)에서 수수는 10 ~ 11월에 수확한 수수로, 새싹을 건조시킨 후 세절기를 이용하여 2cm 이내로 세절하여 얻은 수수새싹을 중량대비 10 내지 20 배수의 증류수를 가하여 중탕조건에서 3 내지 8시간씩 3회 환류추출하고 냉침한다.In the above step (S10), sorghum is sorghum harvested from October to November, and after drying the buds, sorghum buds obtained by slicing within 2 cm by using a slicing machine are added in distilled water of 10 to 20 times the weight, and then 3 The reflux is extracted 3 times for 8 to 8 hours and then cooled.
그 후, 3 내지 8 ㎛ 여과지로 여과하여 추출액을 제조하여 발효 과정의 추출액으로 사용한다.After that, it is filtered through 3 to 8 μm filter paper to prepare an extract and used as an extract in the fermentation process.
다음으로, 상기 수수새싹 추출액에 균주 사카로미세를 접종하여 발효액을 얻는 단계(S20)를 수행한다.Next, a step (S20) of inoculating the strain Saccharomyces in the sorghum sprout extract to obtain a fermentation broth is performed.
상기 단계(S10)에서 얻은 수수새싹 추출액에 균주 사카로미세스와 YPD(Yeast extract, Peptone, Dextrose 2%) Broth 배지를 넣어 25 내지 35 ℃ 호기적 조건에서 48 내지 72 시간 발효를 진행한다. 배양 후 배양액을 원심분리하여 배양균을 1차 제거하고, 이렇게 얻은 여과액을 다시 여과기를 이용하여 0.2 ㎛까지 여과한 후 더 이상 배양되지 않도록 살균 처리를 한다.The strain Saccharomyces and YPD (Yeast extract, Peptone, Dextrose 2%) Broth medium were added to the sorghum sprout extract obtained in the step (S10) to ferment for 48 to 72 hours at 25 to 35°C aerobic conditions. After the culture, the culture medium is centrifuged to remove the culture bacteria first, and the filtrate thus obtained is again filtered to 0.2 μm using a filter, and sterilized to prevent further culture.
상기 단계(S20)에서 수득한 수수새싹 발효액은 화장료 조성물 총 중량에 대해 0.05 내지 20.0 중량%이 포함될 수 있다.Sorghum sprout fermentation broth obtained in the step (S20) may contain 0.05 to 20.0% by weight relative to the total weight of the cosmetic composition.
상기 수수새싹 발효액은 화장료 조성물 총 중량대비 0.05 중량% 미만인 경우 리폭시게나아제, 히아루로니다아제의 활성억제 및 자유라디칼의 소거 효과가 나타나지 않는 문제가 있으며, 20 중량% 초과인 경우 건조 피부를 가진 사람에게 피부 가려움과 따끔거림이 발생되는 문제가 있다. The sorghum sprout fermentation broth has a problem that the activity inhibition of lipoxygenase, hyaluronidase and the scavenging effect of free radicals do not appear when the total weight of the cosmetic composition is less than 0.05% by weight, and if it is more than 20% by weight, people with dry skin There is a problem of skin itching and tingling.
그 다음으로, 상기 단계(S20)의 수수새싹 발효 후 환류중탕추출법을 이용하여 추출하여 침적 후 배양균을 제거한 수수새싹 발효 추출액을 얻는 단계(S30)를 수행한다.Next, after the fermentation of sorghum sprout in the step (S20) and extracting using the reflux middle hot water extraction method to perform the step (S30) to obtain a fermentation extract of sorghum sprout after removal of the cultured bacteria after deposition.
상기 단계(S20)에서 수득한 수수새싹 발효액을 멸균(121℃, 15분, 1.5기압)하여 더 이상 배양되지 않도록 한 후 30%(V/V) 알코올을 2배수 첨가하여 3 내지 6시간 씩 3회 환류중탕추출하고 냉침한 후 원심부리하여 배양균을 1차 제거하고, 이렇게 얻은 여과액을 다시 여과지를 이용하여 0.2㎛까지 여과하여 수수새싹 발효 추출액을 얻는다. After sterilizing the sorghum sprout fermentation broth obtained in the step (S20) (121° C., 15 minutes, 1.5 atmospheres) to prevent further culture, 30% (V/V) alcohol is added in multiples of 2 to 3 to 6 hours 3 After extracting the reflux and centrifuging it after cooling, the culture bacteria are first removed, and the filtrate thus obtained is filtered again to 0.2 µm using filter paper to obtain a fermented extract of sorghum sprout.
상기 단계(S30)에서 수득한 수수새싹 발효 추출액은 감압농축 및 동결전조하여 화장료 조성물 총 중량에 대해 0.05 내지 20.0 중량%가 포함될 수 있다.The sorghum sprout fermentation extract obtained in the step (S30) may be included in a concentration of 0.05 to 20.0% by weight relative to the total weight of the cosmetic composition by concentration under reduced pressure and freeze rolling.
상기 수수새싹 발효 추출액은 화장료 조성물 총 중량대비 0.05 중량% 미만인 경우 리폭시게나아제, 히아루로니다아제의 활성억제 및 자유라디칼의 소거 효과가 나타나지 않는 문제가 있으며, 20 중량% 초과인 경우 건조 피부를 가진 사람에게 피부 가려움과 따끔거림이 발생되는 문제가 있을 수도 있다. The sorghum sprout fermentation extract has a problem that the activity inhibition of lipoxygenase, hyaluronidase, and the scavenging effect of free radicals does not appear when the total weight of the cosmetic composition is less than 0.05% by weight, and when it is more than 20% by weight, it has dry skin. People may have skin itching and tingling problems.
이어서, 상기 단계(S20)의 수수새싹 발효액 또는 상기 단계(S30)의 수수새싹 발효 추출액에 안정화제, 비타민, 안료, 색소 및 향료를 포함하여 화장료 조성물을 얻는 단계(S40)를 수행할 수 있다.Subsequently, a step (S40) of obtaining a cosmetic composition including a stabilizer, a vitamin, a pigment, a pigment, and a fragrance may be performed in the sorghum sprout fermentation liquid of the step (S20) or the sorghum sprout fermentation extract of the step (S30).
상기 단계(S40)에서 안정화제, 비타민, 안료, 색소 및 향료는 당해 화장료 분야에서는 공지된 구성이며 화장료의 종류에 따라 다양할 수 있는바, 본 발명의 기술적 사상의 명확화 및 설명의 편의를 위하여 상기 안정화제, 비타민, 안료, 색소 및 향료에 대한 구체적인 설명은 생략하기로 한다.In the step (S40), stabilizers, vitamins, pigments, pigments, and fragrances are well-known components in the cosmetic field and may vary depending on the type of cosmetic, for clarity of the technical idea of the present invention and convenience of explanation. Detailed descriptions of stabilizers, vitamins, pigments, pigments and flavors will be omitted.
이하, 본 발명에 따른 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물에 대한 제조예, 실시예, 비교예, 시험예 및 제형예를 들어 더욱 구체적으로 설명하기로 한다.Hereinafter, a preparation example, an example, a comparative example, a test example and a formulation example for a cosmetic composition for improving skin wrinkles containing a fermentation broth of a sorghum sprout extract according to the present invention or a fermentation extract thereof as an active ingredient, more specifically Shall be
하기 제조예, 실시예, 비교예, 시험예 및 제형예는 본 발명을 예시하는 것일뿐, 본 발명이 하기 제조예, 실시예, 비교예, 시험예 및 제형예에 한정되는 것이 아니다.The following Preparation Examples, Examples, Comparative Examples, Test Examples, and Formulation Examples are merely illustrative of the present invention, and the present invention is not limited to the following Preparation Examples, Examples, Comparative Examples, Test Examples, and Formulation Examples.
[제조예] 수수새싹 추출액 제조방법[Production Example] Sorghum sprout extract manufacturing method
수수새싹을 정제수로 세척하고 건조한 다음 2cm 이내로 세절하여 세절된 시료를 얻었다. 세절된 수수새싹 50g/L가 되도록 증류수 가하고 중탕 조건에서 8시간씩 3회 환류추출하고 냉침하였다. 그 후, 6㎛의 여과지(와트만(whatman) #3)로 여과하여 추출액을 제조하여 발효 과정의 추출액으로 사용하였다.The sorghum bud was washed with purified water, dried, and then shredded within 2 cm to obtain a shredded sample. Distilled water was added so that the shredded sorghum sprout was 50 g/L, reflux was extracted 3 times for 8 hours in a hot water condition, and then cooled. Thereafter, an extract was prepared by filtration with a filter paper of 6 μm (whatman #3) and used as an extract in the fermentation process.
또한, 상기의 추출액을 50 ℃ 이하에서 감압농축 및 동결 건조하여 효능 평가 시에 사용하였다.In addition, the extract was concentrated under reduced pressure and freeze-dried at 50° C. or lower, and used for efficacy evaluation.
[실시예] 수수새싹 발효액 및 수수새싹 발효 추출액의 제조방법[Example] Manufacturing method of fermented sorghum sprout and fermented extract of sorghum sprout
상기 제조예에서 제조된 수수새싹 추출액에 균주 Saccharomyces cerevisiae(KCTC 7917)와 YPD Broth배지를 넣어 30℃ 호기적 조건에서 60 시간 발효를 진행한다. 발효 후 더 이상 배양되지 않도록 멸균(121℃, 15분, 1.5기압)한 후 수수새싹 발효물에 30%(V/V) 알코올을 첨가하여 5시간씩 3회 환류중탕추출하고 냉침한다.The strain Saccharomyces cerevisiae (KCTC 7917) and YPD Broth medium were added to the sorghum sprout extract prepared in the preparation example, and fermentation was performed for 60 hours at 30°C aerobic conditions. After fermentation, after sterilization (121°C, 15 minutes, 1.5 atmospheres) so that it is no longer cultured, 30% (V/V) alcohol is added to the fermented sorghum sprouts, and the mixture is refluxed 3 times for 5 hours and cooled.
상기 냉침한 발효배양액을 원심분리하여 배양균을 1차 제거하고, 이렇게 얻은 여과액을 다시 여과기를 이용하여 0.2㎛ 까지 여과하였다. 여과하여 얻어진 수수새싹 발효 추출액을 농축조로 이송하여 50℃ 이하에서 감압농축 및 동결 건조하였다.The cold-fermented fermentation broth was centrifuged to first remove the cultured bacteria, and the filtrate thus obtained was again filtered to 0.2 μm using a filter. The sorghum sprout fermentation extract obtained by filtration was transferred to a concentration tank and concentrated under reduced pressure and freeze-dried at 50°C or lower.
[비교예] 사카로미세스 배양액 제조방법의 비교예[Comparative Example] Comparative example of Saccharomyces culture method
본 비교예에서는 실시예에서 사용된 발효균주 Saccharomyces cerevisiae(KCTC 7917)을 YPD broth 배지에 30 ℃ 호기적 조건에서 60 시간 배양을 진행한 후 더 이상 배양되지 않도록 멸균(121℃, 15분, 1.5기압)한다.In this comparative example, the fermentation strain Saccharomyces cerevisiae (KCTC 7917) used in the Examples was cultured in YPD broth medium for 60 hours at 30°C aerobic conditions and sterilized to prevent further culture (121°C, 15 minutes, 1.5 atmospheres). )do.
멸균 후 원심분리하여 배양균을 1차 제거하고, 이렇게 얻은 여과액을 다시 여과기를 이용하여 0.2㎛ 까지 여과한 후 배양균을 제거한 배양액을 농축조로 이송하여 50℃ 이하에서 감압농축 및 동결 건조하였다.After sterilization, the cultured bacteria were first removed by centrifugation, and the filtrate thus obtained was again filtered to 0.2 µm using a filter, and then the cultured liquid from which the cultured bacteria were removed was transferred to a concentration tank, concentrated under reduced pressure at 50° C. or freeze-dried.
[시험예 1] 본 발명의 수수새싹 발효액에 의한 세포 증식 및 독성 확인[Test Example 1] Cell proliferation and toxicity confirmation by sorghum sprout fermentation broth of the present invention
< 세포 배양 ><Cell culture>
상기 실시예, 비교예에서 제조된 수수새싹 발효액 건조분말과 사카로미세스 배양액 건조분말 및 제조예에서 제조한 건조분말의 항노화에 작용하는 유전자 발현을 확인하기 위하여 콜라겐(Collagen)와 Timp-1는 HaCaT(Human keratinocyte cell line)을 사용하고, MMP-1와 트로포엘라스틴(Tropoelastin)은 CCD-986Sk(Human fibroblast cell line)을 사용하였다. HaCaT, CCD-986Sk 세포는 10%(v/v)의 FBS(fetal bovine serum, Gibco, USA), 100U/mL의 streptomycin, 100U/mL의 penicillin 항생제(Gibco, USA)가 포함된 DMEM(Dulbecco's modified eagle's medium, Gibco, USA)배지를 사용하여 5% CO2 및 37℃ 조건의 인큐베이터에서 배양하였다.In order to confirm the gene expression acting on the anti-aging of the dried powder of Sorghum sprout fermentation broth and the dried powder of Saccharomyces prepared in the above Examples and Comparative Examples, and collagen and Timp-1, HaCaT (Human keratinocyte cell line) was used, and MMP-1 and Tropoelastin were used as CCD-986Sk (Human fibroblast cell line). HaCaT, CCD-986Sk cells contain 10% (v/v) of FBS (fetal bovine serum, Gibco, USA), 100 U/mL of streptomycin, and 100 U/mL of penicillin antibiotic (Gibco, USA) with DMEM (Dulbecco's modified) eagle's medium, Gibco, USA) medium, and cultured in an incubator at 5% CO 2 and 37°C.
< 세포 생존율 측정 실험(MTT assay) ><Experiment for measuring cell viability (MTT assay)>
상기 실시예, 비교예에서 제조된 수수새싹 발효액 건조분말과 사카로미세스 배양액 건조분말 및 제조예에서 제조한 건조분말에 대하여 세포 증식에 미치는 영향을 확인하였다.The effects on the cell proliferation of the dry powder of sorghum sprout fermentation broth prepared in Examples and Comparative Examples, the dry powder of Saccharomyces culture and the dry powder prepared in Preparation Example were confirmed.
세포 증식 및 독성 확인을 위하여 하기와 같은 방법으로 실험을 진행하였다. 배양하고 있는 세포 3T3(fibroblast cell line)을 96 웰 마이크로플레이트에 5,000 세포/웰로 분주(分株)하여 30분간 항온조에서 배양하고, 시료를 농도 별 각각 0.01, 0.05, 0.1, 0.5(%, W/V))로 투여하여 72시간 동안 배양하였다. 72시간 배양 후, 티아졸린 블루(Thiazoline blue)를 투여하고 4시간 동안 추가 배양을 하였다.In order to confirm cell proliferation and toxicity, experiments were conducted as follows. The cultured cell 3T3 (fibroblast cell line) is divided into 5,000 cells/well in a 96-well microplate and cultured in a thermostat for 30 minutes, and the samples are 0.01, 0.05, 0.1, 0.5 (%, W/, respectively) for each concentration. V)) to incubate for 72 hours. After incubation for 72 hours, thiazolin blue was administered and further cultured for 4 hours.
배양액을 모두 버리고 마이크로플레이트의 각 웰에 반응 정지액을 가하고 5분간 교반한 후, 570 ㎚에서 흡광도를 측정하였다. 대조군은 시료 주입량만큼 10% 우태아혈청(Fetal Bovine Ser㎛, FBS) 배지를 투여하여 세포 성장의 최적 조건으로 동시배양을 하였으며, 대조군의 세포증식을 100 %로 하고 시료 투입 실험군의 세포 증식율을 계산하였다.The culture solution was discarded, the reaction stopper solution was added to each well of the microplate, and the mixture was stirred for 5 minutes, and absorbance was measured at 570 nm. As a control group, 10% fetal bovine serum (FBS) medium was administered at the same time as the sample injection amount, and co-culture was performed under optimal conditions for cell growth. The cell proliferation of the control group was set to 100% and the cell proliferation rate of the sample injection experiment group was calculated. Did.
세포증식 효과는 계산식 1에 의해 산출되었고, 그 결과는 하기 표 1에 나타내었다.The cell proliferation effect was calculated by the calculation formula 1, and the results are shown in Table 1 below.
[계산식 1][Calculation formula 1]
상기 [표 1]을 참조하면, 세포 성장 최적 조건에서의 세포 증식을 100%로 하였을 때, 실시예와 비교예에서 제조된 수수새싹 발효액 건조분말과 사카로미세스 배양액 건조분말 및 제조예에서 제조된 수수새싹 추출액 건조분말 모두 세포 독성이 없었다.Referring to [Table 1], when cell proliferation under the optimal conditions for cell growth was 100%, dried sorghum sprout fermentation broth prepared in Examples and Comparative Examples and Saccharomyces culture broth dried powder and prepared in Preparation Example Dry powder of Sorghum sprout extract was not cytotoxic.
특히, 실시예와 제조예에서는 세포 증식효과가 있음을 확인하였고, 실시예는 제조예보다 월등한 세포증식효과를 나타내어 가장 우수하였다. 반면, 비교예의 경우 세포독성은 없었으나 세포증식효과 또한 없는 것으로 확인되었다.In particular, it was confirmed that the cell proliferation effect was found in the Examples and Production Examples, and the Examples showed the superior cell proliferation effect than the Production Examples, and were the best. On the other hand, in the case of the comparative example, it was confirmed that there was no cytotoxicity, but also no cell proliferation effect.
[시험예 2] 세포내 collagen, Timp-1 및 Tropoelastin의 유전자 발현과 MMP-1의 유전자 발현 억제 효과[Test Example 2] Intracellular collagen, Timp-1 and Tropoelastin gene expression and MMP-1 gene expression inhibitory effect
HaCaT와 CCD-986Sk 세포는 세포의 수를 5×105 cells/㎖로 조제한 후에 페트리디쉬에 처리하여 24시간 동안 5% CO2 및 37 ℃의 조건에서 배양하였다. 세포가 페트리디쉬에서 80% 이상 자란 다음, 추출물 세포 생존률 100% 이상으로 나타난 농도로 첨가하였다.HaCaT and CCD-986Sk cells were prepared at 5×10 5 cells/ml, and then treated with petri dishes to incubate at 5% CO 2 and 37° C. for 24 hours. Cells were grown to 80% or more in Petri dishes, and then added to a concentration indicated by 100% or more of the cell viability of the extract.
실시예와 비교예에서 제조된 수수새싹 발효액 건조분말과 사카로미세스 배양액 건조분말 및 제조예에서 제조된 수수새싹 추출액 건조분말을 처리한 후 DMED/10 FBS에서 24시간 동안 5% CO2 및 37 ℃의 조건에서 배양하였다. 24시간 후 세포에서 RNA 분리, cDNA 합성 및 RT-PCR은 Komoroski 등(Drug Metab. Dispos. 32:512-518, 2004)이 기술한 방법에 따라 수행하였다. RT-PCR에서 측정한 유전자는 COL1A1, TIMP-1 및 Tropoelastin의 발현과 MMP-1의 발현 억제를 확인하였다. 유전자 발현의 차이를 확인하기 위해 house keeping gene으로 GAPDH을 사용하였다.5% CO 2 and 37° C. for 24 hours in DMED/10 FBS after treating the dry powder of sorghum sprout fermentation broth prepared in Examples and Comparative Examples and the dry powder of Saccharomyces culture and dry sorghum sprout extract prepared in Preparation Example It was cultured in the conditions of. After 24 hours, RNA isolation from cells, cDNA synthesis, and RT-PCR were performed according to the method described by Komoroski et al. (Drug Metab. Dispos. 32:512-518, 2004). Genes measured in RT-PCR confirmed the expression of COL1A1, TIMP-1 and Tropoelastin and suppression of MMP-1 expression. GAPDH was used as a house keeping gene to confirm the difference in gene expression.
각각의 유전자의 프라이머 서열은 표 2에 나타냈다. 추출된 RNA를 주형으로 올리고 dT-어댑터 프라이머(Oligo dT-adaptor primer)와 DEPC water를 이용하여 총량 15㎕로 하고 70℃ 5분 변성후, M-MLV(Moloney murine leukemia virus) RNA의 역전사를 수행하여 42℃에서 60분, 94℃에서 5분 동안 cDNA를 합성하였다.The primer sequence of each gene is shown in Table 2. Raise the extracted RNA as a template and make a total amount of 15 µl using dT-adapter primer and DEPC water, and after 5 minutes denaturation at 70°C, reverse transcription of M-MLV (Moloney murine leukemia virus) RNA Thus, cDNA was synthesized for 60 minutes at 42°C and 5 minutes at 94°C.
합성된 cDNA는 PCR 반응을 통해 증폭하였으며, 총 반응액은 25㎕로 하였다. 각 반응물의 최종 농도는 프라이머 100p㏖, 1.0μM, dNTP 혼합액 0.2mM, 5x green or colorless GoTaq Reaction buffer 1×1.5mM, MgCl2(PCR 완충액) 및 GoTaq DNA 폴리머라제 1.25units 이었다.The synthesized cDNA was amplified through a PCR reaction, and the total reaction solution was 25 μl. The final concentration of each reaction was 100 pmol of primer, 1.0 μM, 0.2 mM of dNTP mixture, 5× green or colorless GoTaq Reaction buffer 1×1.5 mM, MgCl 2 (PCR buffer) and 1.25 units of GoTaq DNA polymerase.
변성(Denaturation), 어닐링(annealing) 및 익스텐션(extension)에 대한 PCR 반응 조건은 다음과 같으며, 95℃에서 2분, 95℃에서 30초, 60℃에서 30초, 72℃에서 1분, 72℃에서 5분 동안, 30~40 사이클로 수행하였다. PCR 반응 후 생성물의 10 ㎕를 2% 아가로즈 겔(agarose gel)에서 전기영동 하였다. Gel Documentation system(코리아랩텍, Cat. No DGS-200D)을 이용하여 발현양을 확인했다.PCR reaction conditions for denaturation, annealing, and extension are as follows, 2 minutes at 95°C, 30 seconds at 95°C, 30 seconds at 60°C, 1 minute at 72°C, 72 At 5° C. for 5 minutes, 30-40 cycles were performed. After the PCR reaction, 10 μl of the product was electrophoresed on a 2% agarose gel. The expression level was confirmed using a Gel Documentation system (Korea Labtech, Cat. No DGS-200D).
(%)Collagen expression
(%)
expression
(%)TIMP-1
expression
(%)
expression
(%)Tropoelastin
expression
(%)
expression
(%)MMP-1
expression
(%)
상기 [표 3] 내지 [표 6]을 참조하면, PT-PCR 수행 후 관찰결과, 본 발명의 실시예는 비교예에서 제조된 사카로미세스 배양액 건조분말 및 제조예에서 제조한 수수새싹 추출액 건조분말 보다 collagen 및 TIMP-1의 발현이 증가하였고, MMP-1의 발현은 농도 의존적으로 감소하는 경향을 확인할 수 있었다. 대조군인 아데노신(Adenosine)과 비교했을 때 우수하거나 비슷한 결과를 확인할 수 있었다. Referring to [Table 3] to [Table 6], observation results after performing PT-PCR, Examples of the present invention are dried powder of Saccharomyces culture powder prepared in Comparative Example and sorghum sprout extract prepared in Preparation Example The expression of collagen and TIMP-1 was increased, and the expression of MMP-1 was decreased in a concentration-dependent manner. When compared with the control group, adenosine, excellent or similar results were found.
또한, 트로포엘라스틴(Tropoelastin)은 피부 탄력을 나타내는 탄력 섬유 중심의 엘라스틴을 형성하고 있는 인자로 실시예 1에서 대조군 Adenosine과 비슷한 결과를 확인할 수 있었다.In addition, Tropoelastin (Tropoelastin) is a factor that forms the elastic fiber-centered elastin showing skin elasticity, it was confirmed that the results similar to the control Adenosine in Example 1.
[제형예 1] 본 발명의 수수새싹 발효액을 함유하는 화장수의 제조 [Formulation Example 1] Preparation of cosmetic water containing sorghum sprout fermentation broth of the present invention
실시예의 수수새싹 발효액 건조 분말을 함유한 화장료 중 화장수(스킨로션)의 제형예는 [표 7]과 같다.The formulation example of the cosmetic lotion (skin lotion) in the cosmetic composition containing the dry powder of the sorghum sprout fermentation liquid of the example is shown in [Table 7].
상기 화장수를 제조하기 위하여, [표 7]에 기재된 11번에 2, 3, 4 및 8번을 순서대로 투입하고 교반하여 용해시킨 후, 5번을 60℃ 정도 가열하여 용해시킨 후, 10번을 투입하여 용해한 후 11번에 투입하였다. 마지막으로 1, 6, 7 및 9번을 투입하여 충분히 교반한 뒤 숙성시켰다.To prepare the lotion, 2, 3, 4, and 8 were sequentially added to No. 11 described in [Table 7] and stirred to dissolve, followed by dissolving No. 5 by heating at about 60° C. and then No. 10. It was added and dissolved, and then added to No. 11. Lastly, 1, 6, 7, and 9 were added to sufficiently stir and aged.
[제형예 1] 본 발명의 수수새싹 발효액을 함유하는 영양로션의 제조[Formulation Example 1] Preparation of nutrient lotion containing sorghum sprout fermentation broth of the present invention
실시예의 수수새싹 발효액 건조 분말을 함유한 화장료 중 영양로션의 제형예는 [표 8]과 같다.The formulation example of the nutritional lotion in the cosmetic composition containing dry powder of the sorghum sprout fermentation broth of the embodiment is shown in [Table 8].
상기 영양로션을 제조하기 위하여, [표 8]에 기재된 10, 11, 13 및 16번을 혼합 교반하면서 80 ~ 85℃ 사이로 가열하여 제조부에 투입한 후 유화기를 작용시키고 2, 3, 4, 5, 6, 7, 8, 9 및 12번을 80 ~ 85℃사이로 가열 용해한 후 유화하였다.In order to prepare the nutrient lotion, 10, 11, 13, and 16 described in [Table 8] were mixed and stirred, heated to between 80 and 85° C., and introduced into the production unit to act as an emulsifier and 2, 3, 4, 5 , 6, 7, 8, 9 and 12 were heat-dissolved between 80 and 85°C and then emulsified.
유화가 끝나면 교반기를 이용하여 교반하면서 50℃까지 냉각한 뒤 15번을 투입하고 45℃까지 냉각한 뒤 14번을 투입하고 35℃에 1번을 투입하여 25℃까지 냉각한 뒤 숙성시켰다.After the emulsification is completed, the mixture was cooled to 50°C with stirring using a stirrer, and then 15 times were added, and after cooling to 45°C, 14 times were added, and 1 time at 35°C, followed by cooling to 25°C, and then aged.
[제형예 3] 본 발명의 수수새싹 발효액을 함유하는 영양크림의 제조[Formulation Example 3] Preparation of nutrient cream containing sorghum sprout fermentation broth of the present invention
실시예의 수수새싹 발효액 건조 분말을 함유한 화장료 중 영양크림의 제형예는 [표 9]와 같다.The formulation example of the nutrient cream in the cosmetic composition containing the dry powder of the fermented sorghum sprout of Example is as shown in [Table 9].
상기 영양크림을 제조하기 위하여 [표 9]에 기재된 12, 13, 14 및 16번을 혼합 교반하면서 80 ~ 85℃ 사이로 가열하여 제조부에 투입한 후 유화기를 작용시키고 2, 3, 4, 5, 6, 7, 8, 9, 10 및 11번을 80 ~ 85℃사이로 가열하여 용해한 후 15번을 투입 교반하여 제조부에 투입하고 유화하였다.In order to prepare the nutrient cream, 12, 13, 14, and 16 described in [Table 9] were mixed and stirred, heated to between 80 and 85° C., put into a production unit, and then acted as an emulsifier. 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11 were heated and dissolved between 80 and 85°C, and 15 were added and stirred, and then added to the production unit and emulsified.
유화가 끝나면 교반기를 이용하여 교반하면서 35℃까지 냉각하고 1번을 투입하여 25℃까지 냉각한 뒤 숙성시켰다.When the emulsification is finished, the mixture is cooled to 35°C with stirring using a stirrer, cooled to 25°C by adding No. 1, and aged.
[제형예 4 ] 본 발명의 수수새싹 발효액을 함유하는 에센스의 제조[Formulation Example 4] Preparation of Essence Containing Fermented Sorghum Sprout of the Present Invention
실시예의 수수새싹 발효액 건조 분말을 함유한 화장료 중 에센스의 제형예는 [표 10]과 같다.The formulation example of the essence in the cosmetic composition containing the dry powder of the sorghum sprout fermentation broth of the embodiment is shown in [Table 10].
상기 에센스를 제조하기 위하여 [표 10]에 기재된 2, 3, 4, 5 및 6번을 일정한 온도에서 균질화하여 제조되는 것을 비이온계 양친매성 지질이라 칭한다. 상기 비이온계 양친매성 지질과 1, 7, 8 및 14번을 혼합하고 일정한 온도에서 균질화하여 마이크로플루이다이져를 통과하고 이어 9번을 일정한 온도에서 서서히 접종하여 균질화한 후 다시 마이크로플루이다이져에 재차 통과시켰다 그리고 10, 11, 12 및 13번을 투입하여 분산시켜 안정화하고 숙성시켰다.In order to prepare the essence, those prepared by homogenizing 2, 3, 4, 5 and 6 described in [Table 10] at a constant temperature are referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid was mixed with 1, 7, 8, and 14, homogenized at a constant temperature, passed through a microfluidizer, and then slowly inoculated at 9 at a constant temperature to homogenize, and then back to the microfluidizer. It was passed again and stabilized and aged by dispersing by adding 10, 11, 12, and 13.
본 발명에서 설명한 것은 수수새싹 추출액의 발효액을 유효성분으로 함유하는 피부 주름개선용 화장료 조성물 및 그의 제조방법을 위한 실시예에 불과한 것으로, 본 발명은 상기 실시예에 한정되는 것이 아니라 서로 다른 다양한 형태로 구현될 수 있으며, 단지 본 실시예는 본 발명의 개시가 완전하도록 하고, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에게 발명의 범주를 완전하게 알려주기 위해 제공되는 것이며, 본 발명은 청구항의 범주에 의해 정의될 뿐이다.What has been described in the present invention is only an example for a cosmetic composition for skin wrinkle improvement and a method for manufacturing the same, which contains the fermentation broth of the sorghum sprout extract as an active ingredient, and the present invention is not limited to the above embodiment but in various different forms. It can be implemented, only this embodiment is provided to make the disclosure of the present invention complete, and to fully inform the scope of the invention to those skilled in the art to which the invention pertains, and the present invention is claimed. It is only defined by the category of.
이상, 첨부된 도면을 참조하여 본 발명의 바람직한 일 실시예를 설명하였지만, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 본 발명이 그 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 그러므로 이상에서 기술한 일 실시예는 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다.The preferred embodiments of the present invention have been described above with reference to the accompanying drawings, but those of ordinary skill in the art to which the present invention pertains have other specific forms without changing the technical spirit or essential features of the present invention. It will be understood that it can be practiced. Therefore, it should be understood that the embodiment described above is illustrative in all respects and not limiting.
Claims (9)
Sorghum sprout extract contains fermented Sorghum sprout fermentation broth or fermented extract obtained by fermentation with Saccharomyces cerevisiae, promotes fibroblast proliferation, increases gene expression of Collagen, TIMP-1, Tropoelastin in cells, and MMP- A cosmetic composition for improving wrinkles in skin, comprising a fermentation broth of a sorghum sprout extract or a fermentation extract thereof as an active ingredient, characterized by inhibiting gene expression of 1.
상기 수수새싹은 물, 탄소수 1 내지 4개의 무수 또는 함수 저급 알코올, 아세톤, 에틸아세테이트, 부틸아세테이트 및 1,3-부틸렌 글리콜로 구성된 군으로부터 선택되는 어느 하나의 추출 용매를 사용하여 추출되는 것을 특징으로 하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물.
According to claim 1,
The sorghum sprout is characterized by being extracted using any one extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol. A cosmetic composition for improving skin wrinkles using the fermentation broth of the sorghum sprout extract or the fermentation extract thereof as an active ingredient.
상기 수수새싹 추출액의 발효액 또는 이의 발효 추출액은 화장료 조성물 총 중량에 대해 0.05 내지 20.0 중량%로 포함되는 것을 특징으로 하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물.
The method according to claim 1 or 2,
The fermentation broth of the sorghum sprout extract or the fermentation extract thereof is a cosmetic composition for improving skin wrinkles using the fermentation broth of the sorghum sprout extract or the fermentation extract thereof as an active ingredient, characterized in that it is contained in an amount of 0.05 to 20.0 wt% based on the total weight of the cosmetic composition.
상기 수수새싹 추출액의 발효액 또는 이의 발효 추출액은 피부 주름 개선 효과를 가지며 피부 자극에 대한 부작용이 없는 것을 특징으로 하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물.
According to claim 3,
The fermentation broth of the sorghum sprout extract or the fermentation extract thereof has a skin wrinkle improving effect and has no side effects on skin irritation, a cosmetic composition for improving skin wrinkles using the fermentation broth of the sorghum sprout extract or its fermentation extract as an active ingredient.
상기 수수새싹 추출액에 균주 사카로미세스 세레비지에를 접종하여 발효액을 얻는 단계(S20);
상기 단계(S20)의 수수새싹 발효액에서 배양균을 제거한 후 중탕 추출법을 이용하여 수수새싹 발효 추출액을 얻는 단계(S30); 및
상기 단계(S20)의 수수새싹 발효액 또는 상기 단계(S30)의 수수새싹 발효 추출액에 안정화제, 비타민, 안료, 색소 및 향료를 더 포함하여 화장료 조성물을 얻는 단계(S40)를 포함하는 것을 특징으로 하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물의 제조방법.
Obtaining a sorghum sprout extract (S10);
Inoculating the strain Saccharomyces cerevisiae to the sorghum sprout extract to obtain a fermentation broth (S20);
Removing the cultured bacteria from the sorghum sprout fermentation broth of the step (S20) and obtaining a sorghum sprout fermentation extract using a hot water extraction method (S30); And
It characterized in that it comprises the step (S40) of obtaining a cosmetic composition further comprising a stabilizer, vitamins, pigments, pigments and flavors in the sorghum sprout fermentation broth of the step (S20) or the sorghum sprout fermentation extract of the step (S30) Method for producing a cosmetic composition for improving skin wrinkles using the fermentation broth of the sorghum sprout extract or the fermentation extract thereof as an active ingredient.
상기 단계(S20)에서 수득한 수수새싹 발효액 또는 단계(S30)에서 수득한 수수새싹 발효 추출액은 화장료 조성물 총 중량에 대해 0.05 내지 20.0중량%로 포함되는 것을 특징으로 하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물의 제조방법.
The method of claim 5,
Sorghum sprout fermentation broth obtained in step (S20) or sorghum sprout fermentation extract obtained in step (S30), fermentation broth or fermentation thereof, comprising 0.05 to 20.0% by weight based on the total weight of the cosmetic composition Method for manufacturing a cosmetic composition for improving skin wrinkles using the extract as an active ingredient.
상기 단계(S10)는 상기 수수새싹을 정제수로 세척하고 건조한 다음 2cm 이내로 세절하여 세절된 시료를 얻으며, 상기 수수새싹 세절물에 50 내지 100g/L의 증류수를 가하고 중탕 조건에서 3 내지 8시간씩 3회 환류추출하고 냉침을 한 후, 3 내지 8㎛ 여과지로 여과하여 추출액을 얻는 것을 특징으로 하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물의 제조방법.
The method of claim 5,
In the step (S10), the sorghum bud is washed with purified water, dried, and then shredded to within 2 cm to obtain a shredded sample. 50 to 100 g/L of distilled water is added to the sorghum bud shredded water, and 3 to 8 hours for 3 to 8 hours under hot water condition. After reflux extraction and cold soaking, a method of manufacturing a cosmetic composition for improving skin wrinkles using a fermentation broth of a sorghum sprout extract or a fermentation extract thereof as an active ingredient, wherein the extract is obtained by filtering with 3 to 8 μm filter paper.
상기 단계(S20)는 상기 단계(S10)에서 얻은 수수새싹 추출액에 균주 사카로미세스와 YPD Broth 배지를 넣어 25 내지 35℃ 호기적 조건에서 48 내지 72시간 발효를 진행하며, 배양 후 더 이상 배양되지 않도록 멸균(121℃, 15분, 1.5기압)한 후 수수새싹 발효물에 30%(V/V) 알코올을 첨가하여 5시간씩 3회 환류중탕추출하고 냉침하고, 냉침한 후 원심분리하여 배양균을 1차 제거하여 얻은 여과액을 다시 여과기를 이용하여 0.2㎛까지 여과한 후 멸균 처리를 하는 것을 특징으로 하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물의 제조방법.
The method of claim 5,
In the step (S20), strain Saccharomyces and YPD Broth medium is added to the sorghum sprout extract obtained in the step (S10) to perform fermentation for 48 to 72 hours at 25 to 35°C aerobic conditions, and no further culture after cultivation After sterilization (121℃, 15 minutes, 1.5 atm), 30% (V/V) alcohol was added to the fermented sorghum sprouts, extracted under reflux three times for 5 hours, and then cold-cooled. Preparation of the cosmetic composition for skin wrinkle improvement using the fermentation broth of the sorghum sprout extract or the fermentation extract thereof as an active ingredient after filtering the filtrate obtained by the first removal to 0.2 μm again using a filter Way.
상기 단계(S30)는 상기 단계(S20)에서 수득한 수수새싹 발효액을 30%(V/V) 알코올을 첨가하여 3 내지 6시간씩 3회 환류중탕 추출하고 냉침을 한 후 멸균 및 방부제를 투여 및 원심분리와 3 내지 8㎛ 여과지로 여과하여 상기 배양균을 제거한 수수새싹 발효 추출액을 얻는 것을 특징으로 하는 수수새싹 추출액의 발효액 또는 이의 발효 추출액을 유효성분으로 하는 피부 주름개선용 화장료 조성물의 제조방법.The method of claim 5,
In the step (S30), 30% (V/V) alcohol is added to the sorghum sprout fermentation liquid obtained in the step (S20) to extract the reflux water 3 times for 3 to 6 hours, sterilize and preservative after cold immersion, and A method for manufacturing a cosmetic composition for improving wrinkles of a skin using a fermentation broth of a sorghum sprout extract or a fermentation extract thereof as an active ingredient, wherein the fermentation extract of sorghum sprout is removed by centrifugation and filtration through 3 to 8 µm filter paper to obtain the fermented extract of sorghum sprout.
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