KR102096703B1 - Cosmetic compostion including extracts of fig leaves - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a fig alcohol extract. Specifically, to the fig alcohol extract, further comprising a herbal medicine, persimmon extract, evening primrose oil saponification, sorghum powder extract, pig potato powder extract, cacao bean pulverized, ginseng extract and mung bean extract; The fig leaf extract has antioxidant, anti-inflammatory and whitening activity; The herbal medicine is a fine powder of hangin, Yulmu, Yulpi, Doin, Baekgangjam, sedija, peppermint, Baeknyeong, Baekji and wormwood; The persimmon extract is extracted from persimmon skin and nipple, and is obtained by using a distillation method; The evening primrose oil saponification is saponified by treating the evening primrose oil with any alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide and potassium hydroxide; The water powder extract is 0.5 to 5.0% by weight; The pork potato powder extract is 0.5 to 5.0% by weight; The cacao bean pulverized product is 10 to 10% by weight of 48 to 72 hours of continuous pulverization with no alkali treatment; The ginseng extract refers to a fermented ginseng to increase a specific ginseng saponin substrate; The mung bean extract is obtained by immersing the mung bean powder in an aqueous solution of alcohol, and is related to a cosmetic composition comprising an extract of fig leaf, characterized in that it contains bitexin and isobitexin as active ingredients.

Description

Cosmetic composition containing main extract of fig leaf {Cosmetic compostion including extracts of fig leaves}

The present invention relates to a cosmetic composition comprising a main extract of fig leaf, in detail, herbal extracts, persimmon extract, evening primrose oil saponification, sorghum powder extract, pig potato powder extract, cacao bean crushed, ginseng extract And it relates to a cosmetic composition further comprising a mung bean extract.

Figs are called figs because they do not bloom, but in reality, flowers only bloom inside the fruit, so they do not appear outside. The pulp is an alkaline fruit with a lot of protein and fiber. Since it is a unit fruit, there is no need for moisture, and the fruit mainly reproduces, but since it is not storable and transportable, it is mainly distributed as canned or dried fruits. The varieties are divided into four types: capri type, smiruna type, san pedro type, and normal type, and Korea mainly cultivates a lot of normal type and san pedro type.

Figs are semi-tropical deciduous broad-leaved fruit trees native to the western and Mediterranean coasts of Asia, which are easy to grow and have less pest damage. Cultivation is possible only in areas with an average annual temperature of 15 ℃ and winter temperatures of -9 ℃ or higher. With a long history of cultivation in Greece and Italy worldwide, Korea was introduced through Japan and China, and the current cultivation centers are California, Italy, Turkey, and Portugal in the United States. In particular, Italy and Greece account for 2/3 of the world's total production. Occupy. Currently, economically cultivated places in Korea are limited to some regions of Jeonnam, and are mainly planted and reproduced one or two at home, and are grown on Jeju Island and the southern coast of Korea.

These figs contain various nutrients such as niacin, sodium, protein, sugar, retinol, beta-carotene, vitamin A, B1, B2, B6, C, E, dietary fiber, zinc, folic acid, phosphorus, lipids, iron, potassium, and calcium. It is a plant that has been spotlighted in food science and pharmacy. In addition, it has been well known that these components are extracted and applied to cosmetics.

Herbal medicines such as Hangin, Yulmu, Yulpi, Doin, Baekgangjam, Tosaja, Peppermint, Baekji, Baekji, and Yamwork have been used in Oriental medicine and have been proven several times to have a pharmacological effect on the extract. In addition, it is a material that is in the spotlight in the health functional food and cosmetics industries. And there have been cases of using ingredients extracted from these herbal medicines in cosmetics.

Persimmon is an alkaline fruit rich in sugars such as glucose and fructose. It is rich in astringent tannins, and the content of vitamin C is about 5-20 times higher than that of the same amount of apples and is rich in minerals. In addition, the tap (Diospyros kaki calyx) is a medicinal name and is known as the body. In folk medicine, it is known to be effective in coughing and asthma, chronic bronchitis treatment, stopping daughter bleeding, enuresis, and strengthening blood pressure. It is known to have excellent whitening effect by inhibiting expression and inhibiting melanin production.

In addition, the tannin and proanthocyanidin components contained in the persimmon skin are water-soluble substances known for their antibacterial, antioxidant, and anti-tumor and heavy metal removal activities, and also the persimmon polyphenol component is antioxidant. And antigenotoxic effects.

However, it is common to consume persimmon only and discard the persimmons and persimmon skins, some of which are used as feed or secondary processing, but they are only partially used for recycling, and most persimmons and persimmon peels are used. A considerable amount of silver is being discarded.

Evening primrose oil has been used by Native Americans as a medicine to treat wounds or to treat colds, and is recently consumed as a cosmetic or nutritional supplement. And evening primrose oil is rich in gamma-linolenic acid and linolenic acid, and has various health effects such as improving vascular diseases, lowering cholesterol levels, controlling female hormones, and preventing adult diseases. Gamma-linolenic acid is a type of omega 6, an unsaturated fatty acid, and is not known to be a component of our body. If you have a skin condition such as atopy or skin psoriasis, applying evening primrose oil directly to your skin is also helpful.

The sorghum is 1m long and the butterfly is 5cm long, with large leaves, white middle and clear, no leaves, and the leaves are blackish brown, and it is a common plant distributed throughout the country with hair follicles. It has anti-cancer and antioxidant effects. It is widely used in medicine or cosmetic industry.

Pork potato is a naturalized plant, although it is not known exactly, it is believed to have been introduced to Korea from Europe through China through the 17th century, and grows wild in fields and fields. If you dig under the dry stem, you can find tubers of pig potatoes, and the flowers blooming in the fall are very attractive. As it is said to be good for diabetes among yosai, more and more farms are harvesting and growing natural products.

Cacao is also known as the cocoa logo, and is native to tropical America, with thick stems and a height of 12m. The fruit is long oval-shaped, about 10cm long, divided into 5 pieces, and contains 40-60 seeds. Seeds do not have endosperm and have bloated cotyledon. The seeds contain about 2% theobromin, some caffeine, and 50% fat. When the seeds are taken out of the ripe fruit and fermented for a few days in a wooden barrel, the seeds turn reddish brown and have a unique scent. After washing this with water and drying it is cacao beans, and it is cacao paste that is roasted and powdered. Chocolate is made by adding sugar, milk, and spices to it. It is cocoa that extracts fat by compressing the cacao paste, and the fat obtained here is called cacao butter. Cacao butter is transparent yellow and has a unique scent and flavor, so it is used in margarine, pomade, and medicine. Theobromine is a type of alkaloid and has a strong effect on the kidneys, so it is used as a diuretic. It has been cultivated since BC, and is now cultivated not only in the Americas, but also in tropical parts of Africa, West Africa, India, and Sri Lanka.

Ginseng is the broadest concept including ginseng, white ginseng, red ginseng, wild ginseng, camphor, rice ginseng, and raw ginseng. Ginseng is a natural product that has been used as a highly valuable medicine in Korean medicine. The main functional component of ginseng is ginseng saponin called "ginsenoside", which is specially named only among ginseng saponins among various saponins in plants. The main component of ginseng is ginsenosides, which are saponins, and ginsenosides Rb1, Rb2, and Rc, which are protopanaxadiol systems, and ginsenosides Re, Rg1, Rf, etc., which are protopanaxatriol systems, are known. Representative pharmacological effects of these components include anti-cancer activity, anti-inflammatory, and anti-diabetic effects. In addition, domestic and foreign studies on minor ginsenosides such as Rh2, Rg3, C-K, and F2, which are considered metabolites of ginsenosides, have recently been conducted. However, it is difficult to obtain minor ginsenosides, so studies on pharmacological effects in the future are insufficient, and the comparison of pharmacological effects of various ginsenosides is still insufficient.

Mungbean (Vigna Radiata (L.) Wilczek or Mungbean) has been widely used as an antipyretic or antidote in oriental medicine since ancient times, and in Donguibogam's literature, it is used like soap to make the face look pretty like jade, remove skin impurities, and is effective for dry skin. It removes blemishes, removes sweat bands that occur well in summer, and is effective for swelling, boils, and mumps caused by the penetration of skin filamentous fungi into the stratum corneum. The skin beauty effect of such mung beans is due to vitexin and isovitexin contained in mung beans, and these bioactive substances are a type of flavonoid-C-glycoside. Vitexin and isobitexin components are known to be effective in preventing the accumulation of skin wastes and inhibiting aging because they have the function of removing free radicals, which are toxic components in the body, and sebum of the skin. As a study of these vitexins and isobitexins, a method of separating the active substances contained in the ethanol extract of green beans or separating the vitex and isobitexins contained in the water extract of green beans has been reported, and other natural substances other than green beans As a method of separating bitexin from moss, or isobitexin from rice ethanol extract, and method of separating bitexin and isobitexin from buckwheat ethanol extract.

There have been many cases of using components extracted from various natural products, but in the present invention, synergistic effect compared to when used alone in a combined state with various components above, especially with respect to the antioxidant, anti-inflammatory and whitening activity of the main extract of fig leaf Found out.

As described above, the present invention has an object to provide a cosmetic composition containing various extracts of natural substances, which have been found in the prior art, focusing on antioxidant, anti-inflammatory and whitening activity of the main extract of fig leaf.

In addition, there is an object to provide a cosmetic composition having a synergistic effect when a number of components are mixed compared to a single composition of various natural extract components found in the related art.

As a means for solving the above problems, an embodiment according to the present invention is in a cosmetic composition comprising a main extract of fig leaf; Herbal medicine, persimmon extract, evening primrose oil saponification, sorghum powder extract, pig potato powder extract, cacao bean pulverized, ginseng extract and mung bean extract further comprising; The fig leaf extract has antioxidant, anti-inflammatory and whitening activity; The herbal medicine is a fine powder of hangin, Yulmu, Yulpi, Doin, Baekgangjam, sedija, peppermint, Baeknyeong, Baekji and wormwood; The persimmon extract is extracted from persimmon skin and nipple, and is obtained by using a distillation method; The evening primrose oil saponification is saponified by treating the evening primrose oil with any alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide and potassium hydroxide; The water powder extract is 0.5 to 5.0% by weight; The pork potato powder extract is 0.5 to 5.0% by weight; The cacao bean pulverized product is 1 to 10% by weight of 48 to 72 hours of continuous pulverization with no alkali treatment; The ginseng extract refers to a fermented ginseng to increase a specific ginseng saponin substrate; The mung bean extract is obtained by immersing the mung bean powder in an aqueous alcohol solution, and contains a bitexin and an isobitexin as active ingredients; and is a cosmetic composition comprising a main extract of fig leaf.

Preferably, the fig leaf extract is a cosmetic composition comprising a fig leaf extract, characterized in that it has antioxidant, anti-inflammatory and whitening activity.

More preferably, the antioxidant activity is a cosmetic composition comprising a fig leaf alcoholic extract, characterized by a polyphenol of 5.0 to 46.7 mg / g at a concentration of 0.1 to 1 mg / mL of the main extract of the fig leaf.

More preferably, the antioxidant activity is a cosmetic composition comprising a fig leaf alcoholic extract characterized by ABTS free radical scavenging activity of 3.8 to 5.2% at a concentration of 0.1 to 1 mg / mL of the main extract of a fig leaf.

More preferably, the antioxidant activity is a cosmetic composition comprising a fig leaf alcoholic extract characterized by DPPH free radical scavenging activity of 0.2 to 25.8 mg / g at a concentration of 0.1 to 1 mg / mL of the fig leaf alcoholic extract.

More preferably, the anti-inflammatory activity is a fig characterized in that the IL-6 inhibitory activity is 25.3 to 32.5% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10-50 μg / ml of the main extract of fig leaf It is a cosmetic composition comprising a leaf extract.

More preferably, the anti-inflammatory activity is a fig characterized in that the IL-12 inhibitory activity is 26.1 to 35.4% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10-50 μg / ml of the main extract of fig leaf It is a cosmetic composition comprising a leaf extract.

More preferably, the whitening activity comprises a fig leaf alcoholic extract, characterized in that the activity of inhibiting the expression of tyrosinase at a concentration of 0.265 to 2.5 mg / ml of fig leaf alcoholic extract is 15.7 to 82.5%. It is a cosmetic composition.

As a means for solving the above problems, another embodiment according to the present invention, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, A hand cream, essence, nutrient essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder and rust powder, any one selected from the group consisting of It is a cosmetic composition comprising the main extract of fig leaf of claim 8.

The effect according to the present invention provides a cosmetic composition having a more enhanced cosmetic effect by containing a variety of natural ingredients centered on the antioxidant, anti-inflammatory and whitening activity of the fig leaf composition.

1 is the total polyphenol content of the fig leaf EtOH extract A is a standard curve using gallic acid; B is the total polyphenol content (mg gallic acid quivalent / g sample) of fig leaves derived using a standard curve.
Figure 2 relates to the ABTS free radical scavenging activity content of ethanol extract of fig leaf A is a standard curve using ascorbic acid; B is the scavenging activity of the ABTS radical (mg ascorbic acid quivalent / g sample) of fig leaves derived using a standard curve.
Figure 3 relates to the DPPH free radical scavenging activity content of ethanol extract of fig leaf, A is a standard curve using ascorbic acid and B is a DPPH radical scavenging activity of the fig leaf derived using a standard curve (mg ascorbic acid quivalent / g sample).
Figure 4 relates to the toxicity evaluation of EtOH extract of fig leaf on RAW 264.7 cells, A is a sample concentration of 1 ~ 1,000 μg / mL; B is 10-50 μg / mL.
Figure 5 shows the inhibitory activity of nitric oxide production of ethanol extract of fig leaves in RAPS 264.7 cells induced by LPS.
Figure 6 shows the (A) IL-6 and (B) IL-12 production inhibitory activity of the fig leaf EtOH extract in RAPS 264.7 cells induced by LPS.
FIG. 7 shows a mechanism for inhibiting tyrosinase activity of a fig leaf and a method for obtaining an activity inhibition rate.
Figure 8 shows the activity of whitening from the ethanol extract of fig leaf (A is the tyrosinase inhibitory activity of the standard leaven acid, B is the tyrosinase inhibitory activity of the ethanol extract of the fig leaf).
Figure 9 shows the IC50 values and graphs of Nuruksan and fig leaves.

Fig is a long-established traditional food that is a completely non-polluting fruit that does not use any pesticides. It has excellent pharmaceutical value as a pharmaceutical effect that prevents adult diseases, and has various antimicrobial activities such as ficine and minerals abundantly contained in figs. It is known that there are many substances.

Figs are also used as athlete's foot and anthelmintic agents, and various active substances exist in fig leaves. However, there is little research on extracting fig leaves or fruits, separating pharmacologically active substances, and measuring active effects on each active substance to make them cosmetic compositions.

Therefore, the present inventors came to invent a cosmetic composition containing a main extract of a fig leaf, but augmenting a cosmetic effect by mixing conventionally known substances.

Hereinafter, examples and related descriptions of specific inventions are as follows.

According to an embodiment of the present invention, in the cosmetic composition comprising the main extract of fig leaf; Herbal medicine, persimmon extract, evening primrose oil saponification, sorghum powder extract, pig potato powder extract, cacao bean pulverized, ginseng extract and mung bean extract further comprising; The fig leaf extract has antioxidant, anti-inflammatory and whitening activity; The herbal medicine is a fine powder of hangin, Yulmu, Yulpi, Doin, Baekgangjam, sedija, peppermint, Baeknyeong, Baekji and wormwood; The persimmon extract is extracted from persimmon skin and nipple, and is obtained by using a distillation method; The evening primrose oil saponification is saponified by treating the evening primrose oil with any alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide and potassium hydroxide; The water powder extract is 0.5 to 5.0% by weight; The pork potato powder extract is 0.5 to 5.0% by weight; The cacao bean pulverized product is 10 to 10% by weight of 48 to 72 hours of continuous pulverization with no alkali treatment; The ginseng extract refers to a fermented ginseng to increase a specific ginseng saponin substrate; The mung bean extract is obtained by immersing the mung bean powder in an aqueous alcohol solution, and contains a bitexin and an isobitexin as active ingredients; to provide a cosmetic composition comprising a main extract of a fig leaf.

The herbal medicine, as a fine powder of almonds, Yulmu, Yulpi, Doin, Baekgangjam, Tosaja, peppermint, Baeknyeong, Baekji, and wormwood, as well as exerts a whitening effect, removes blemishes, itchiness, and feels moist and clean after use. To make.

The herbal medicines listed above, namely passersby, freckles, whitening. Skin cleanliness; Yulmu promotes skin metabolism, skin recovery, whitening, skin redox, blemishes, warts; Doin has freckles, freckles, moisturizing, whitening; Baekjanggam is used to remove spots, freckles, appearance, prevention of digestion, and itching; Sediment, spots, freckles, acne, blemishes, and skin moisture; Peppermint boils, swelling, itching, skin diseases, headache, neuralgia, migraine, toothache, blood circulation, fever; White paper shrinks pores, moisturizes skin, removes whitening and itching Wormwood is effective for cell regeneration, antibacterial, anti-inflammatory, blemish, freckle, and acne.

After preparing the listed herbal medicines, washed with purified water and dried, filled with 100% by weight of ethanol inside the pottery jar, immersed in the dried herbal drugs listed above, and tightly sealed the top of the pottery jar with the outside. The sealed pottery jars are stored in a sun-free warehouse for 2-5 days to sterilize the herbal drugs listed above, and when sterilized, remove the listed herbal drugs from the pottery jars and filter and dry them. The dried herbal medicine is obtained by using a separate grinder to finely powder the herbal medicine into 170-200 mesh.

The finely divided herbal drug is preferably 3 to 5% by weight. Below 3% by weight, it is difficult to expect a whitening effect and a removal effect such as blemishes, itching, etc. If it is more than 6% by weight, the whitening effect and removal effects such as blemishes, itching, etc. are excellent, but the cleaning power is lowered and it is uneconomical. It is more preferably 5% by weight.

The persimmon extract is an extract from persimmon peels and nipples that have excellent skin whitening and antibacterial activity, but has skin exfoliation function, and is obtained by using a distillation method. And mixed with caustic soda, washed and dried to obtain an extract using a distillation method.

The tannin and proanthocyanidin components contained in the persimmon peel and the extract of the persimmon are water-soluble substances and have antibacterial, antioxidant, antitumor, and heavy metal removal effects, resulting in skin trouble due to excellent antibacterial and antioxidant effects You can prevent it.

It is preferable to add 0.5 to 3% by weight of the extract obtained from the persimmon peel. When the persimmon skin crushed material is added in an amount of less than 0.5% by weight, the scrub effect of the soap is lowered, which is not preferable.

The extract obtained from the nipple is preferably 2 to 30% by weight. If it is added in less than 2% by weight, the whitening function of the extract of the nipple is insignificant and undesirable, and when it is added in excess of 30% by weight, the color of the soap becomes excessively dark and odor may be generated, which is not preferable.

The evening primrose oil saponified is saponified by treating the evening primrose oil with any one alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide and potassium hydroxide.

The evening primrose oil refers to the oil component obtained by physically crushing the evening primrose seeds, and the saponification is a reaction to generate carboxylic acids and alcohols by hydrolyzing the ester bonds in the oil and fat components contained in the evening primrose oil. In other words, it means the reverse reaction of the esterification reaction, and the "saponifiable" refers to an alkali salt of a fatty acid hydrolyzed by treating the ester group bond in the oil component contained in the evening primrose oil with a "saponification reagent". The "saponification reagent" which can be used is preferably an alkali metal hydroxide such as sodium hydroxide, potassium hydroxide or lithium hydroxide.

The evening primrose oil saponification product has an excellent skin whitening effect through suppression of melanin pigment production, and is effective in treating and preventing skin pigmentation diseases caused by excessive production of melanin pigment.

The evening primrose oil saponified product is a mixture of evening primrose oil and 2.5M KOH solution dissolved in ethanol in a 2: 1 volume ratio, and heated to 80 ° C to prepare evening primrose oil saponified product.

In order to confirm that the evening primrose oil saponification reduces melanin production, melanoclast cells are treated with IBMX (Isobutylmethylxanthine) to create an environment that promotes melanin production, and evening primrose oil saponification is compared to the control group to produce melanin. It was confirmed to decrease.

Human melanoma cells were treated with IBMX at a concentration of 0.1 mM to promote the production of melanin pigment in melanoma cells, and evening primrose seed oil saponified at a concentration of 0 to 100 ug / ml. The evening primrose oil saponified human melanoma cells were cultured for 3 days.

In order to measure the melanin pigment content of the cultured melanoma cells, the cells were washed with physiological saline, dissolved in lysis buffer, and treated with 20% TCA solution. The precipitate was washed twice with 10% TCA solution, and then treated sequentially with a mixed solution of ethanol and diethyl ether (3: 1), and diethyl ether solution, dried in air, and then 0.85M potassium hydroxide. Dissolved in solution and heated for 15 minutes. After cooling, absorbance was measured at 440 nm. In order to correct the difference in melanin pigment content according to the number of cells, the protein is quantified by the Bradford method (Anal Biochem 1976; 72: 248-54), and the measured amount of melanin pigment is divided by the amount of protein per 1 mg of protein The melanin pigment production amount (melanin pigment measurement / protein) is determined.

As a result, the non-saponified evening primrose oil treatment control group did not significantly decrease the production of melanin pigment even when the dose was increased to a concentration of 100 ug / ml.

On the other hand, in the group treated with the evening primrose oil saponification, the melanin content generated in the cells was reduced depending on the treatment capacity, and it was observed that the melanin production was inhibited by about 90% at a concentration of 100.0 ug / ml.

As a result of the above cell experiment, it was found that the evening primrose oil saponification has an excellent effect in suppressing the production of melanin pigment in real cells.

The aqueous powder extract and the pig potato powder extract are 0.5 to 5.0% by weight, and a phenol compound, proanthocyanin and vitamin B that improve skin moisture are extracted.

The sorghum powder extract and the pig potato powder extract were used as raw materials, and then extruded, cut, and struck in a soap making machine to prepare a cosmetic soap composition in the form of a solid soap and tested for skin irritation and skin moisturization.

The skin irritation experiment was conducted with 20 subjects. A series of evaluation criteria (0: no reaction to the skin; 1: partial skin pulmonary rash; 2: overall skin pulmonary rash; 3: skin pulmonary rash and skin Swells; 4: Prepares skin thorax and severely cracks the skin) and averages the results.

Since the skin moisturization experiment changes makeup residues and wastes to the moisture level, it was measured with a moisture meter after 2 hours after washing. And when pressed against the skin part to be measured, the moisture content is checked on the LCD display panel that senses the electronic action, that is, the skin moisture. The display unit is%, and if the moisture is 37% or less, it is very dry, and the average is 37 to 47%. The judgment was conducted on 20 subjects and the results were averaged.

As a result of checking the degree of skin irritation, it was found that there was no reaction to the skin, or the skin chest was partially caused. And after 2 hours after washing, the skin moisture was checked with a moisture meter. As a result, it was checked to be 55 to 78%, which is higher than that of the normal person, 37 to 47%.

The cacao bean pulverized product is 1 to 10% by weight, which is continuously pulverized for 48 hours to 72 hours by not treating alkali, and the cacao bean contains a large amount of polyphenols, which are anti-aging and antioxidant components, and 48 to 72 hours in a grinder. After continuous grinding until time, the raw cacao beans are roasted at 200 degrees for 20 to 30 minutes before crushing, and then the cacao beans are shelled and then crushed and used without mixing with an alkali agent.

When making foods such as chocolate using cacao beans, cacao beans are removed from the shell immediately after roasting and mixed with an alkali agent to neutralize the taste such as sourness, but in the present invention, cacao is crushed immediately without mixing an alkali agent. Maximize the polyphenol content of soybeans.

Experiments were conducted with different roasting temperatures and times with respect to the polyphenol content.

Total polyphenols from Sample 1 (roast for 20 minutes at 150 degrees), Sample 2 (roast at 150 degrees for 30 minutes), Sample 3 (roast at 200 degrees for 25 minutes), and Sample 4 (roast at 200 degrees for 30 minutes) The contents were 14.5 mgGAE / g, 15.3 mgGAE / g, 16.8 mgGAE / g, and 15.9 mgGAE / g, respectively. Therefore, the total polyphenol content of cacao beans roasted at 200 degrees for 25 minutes was the highest.

The cacao bean pulverized product is 1 to 10% by weight, but preferably, it is sufficient to add it in the range of 5 to 8% by weight. If it exceeds the content, it is difficult to obtain the intended effect in the present invention, and when the content of each component is exceeded, it is difficult to obtain the desired effect, and rather, it may irritate the skin and adversely affect the skin. Is insufficient and the desired result cannot be obtained.

The ginseng extract refers to an increase in a specific ginseng saponin substrate by fermenting ginseng, thereby suppressing skin aging and improving wrinkles.

The fermented ginseng extract is characterized by mixing at 0.01 to 50% by weight.

The fermented ginseng extract is prepared by mixing and incubating yeast-derived bacteria, malt, wheat bran, and water that are active in beta-glucosidase, fermenting ginseng, and extracting food, pharmaceutical, and cosmetic compositions to inhibit skin aging It can be formulated as dragon, skin wrinkle improvement, skin whitening and pore reduction, sebum reduction, skin moisturizing food, medicine and cosmetics.

The fermented ginseng extract was cultured in a culture medium in which potato dextrose broth (PDB) and corn meal broth were mixed, and a strain isolated from yeast was cultured to prepare a strain suspension. The prepared ginseng is typically mixed with rice flour and prepared and then put into a fermenter. 100 g of prepared rice flour, 100 g of ginseng, 100 g of water, and 50 g of a strain suspension were mixed and put into a fermenter to ferment at a temperature of 30 ° C. and 70% moisture to obtain an extract.

And as a control, an extract was obtained using ginseng without any treatment.

Sensory evaluation was performed using the fermented ginseng extract and a ring made using the control. Twenty healthy women and men aged 20 to 50 years were to take pills made with fermented ginseng three times a day for two weeks. After 2 weeks, the degree of skin aging inhibition and wrinkle improvement was evaluated through a subject survey. The subjects were judged as three stages of no improvement, slight improvement, and significant improvement, and as a result, significantly improved (9 patients), slight improvement (7 patients), and no improvement (4 patients) for skin aging inhibition; As for the degree of skin wrinkle improvement, it was counted as significantly improved (10 people), slightly improved (8 people), and no improvement (2 people), and it was confirmed that when taking the fermented ginseng extract, it has the effect of inhibiting skin aging and improving wrinkles.

The mung bean extract is obtained by immersing the mung bean powder in an aqueous solution of alcohol, and contains vitexin and isobitexin as active ingredients, and has excellent antioxidant effects, such as removing skin blemishes such as spots, freckles, and improving wrinkles.

The mung bean powder is first prepared by washing mung beans with clean water, removing impurities, etc., and then drying and sufficiently crushing the mung beans.

Thereafter, the crushed mung beans were weighed to be 1 kg, and then precipitated in an aqueous solution mixed with alcohol and water at 7: 3 and left for 5 days. Preferably, the alcohol is ethanol.

The neglected precipitate was filtered using a 300 mesh filter cloth, and the filtrate was concentrated at 70 ° C using a vacuum reduced pressure concentrator to obtain a mung bean extract.

Antioxidant action of the concentrated mung bean extract, that is, to see if there is an effect of removing skin blemishes such as blemishes and freckles, and improving wrinkles, separately prepare a green bean extract from which vitexin and isobitexin are removed as a control, and the concentrated green bean extract And the sensory evaluation was performed using the mask pack made using the control group. Mask packs made using the concentrated mung bean extract and the control group were used for 20 healthy females and 20 males aged 20 to 50 years once a day for 2 weeks. After 2 weeks, the effects of removing skin blemishes such as blemishes and freckles and improving wrinkles were evaluated through a subject survey. The subjects were judged as three stages of no improvement, slight improvement, and significant improvement, and as a result, significant improvement (10 persons), slight improvement (8 persons), and no improvement for the skin blemish removal effect from the concentrated mung bean extract (2 people); Regarding the degree of improvement in skin wrinkles, it was counted as significantly improved (11 persons), slightly improved (5 persons), and no improvement (4 persons). Antioxidative effect on the concentrated mung bean extract, that is, removal of skin blemishes such as spots, freckles, and wrinkle improvement It was confirmed that the effect of the back.

The main extract of fig leaf has antioxidant, anti-inflammatory and whitening activity.

The antioxidant activity is due to the polyphenol of the fig leaf alcoholic extract, preferably from 5.0 to 46.7 mg / g of polyphenol at a concentration of 0.1 to 1 mg / mL of the fig leaf alcoholic extract.

There are more than thousands of types of polyphenols. In general, polyphenols are known to prevent aging because they have antioxidant effects that can remove free radicals from our body. In addition, it has been reported to reduce the risk of various diseases because of its excellent function of protecting DNA damaged by exposure to free radicals, protecting cellular components, and enzymes. It is also known to prevent heart disease along with its anti-cancer action.

In order to analyze the content of polyphenols contained in the main extract of fig leaf, the present inventors developed blue to yellow when the Folin-Ciocalteus' reagent is reduced by the polyphenol compound of the sample under alkaline conditions. It was measured using the principle.

That is, 2 mL of 2% Na ₂ CO ₃ was added to 100 μL of the sample to form alkali conditions, followed by reaction for 3 minutes, and 100 μL of 50% polyline-Siocalteus reagent was added for reaction for 30 minutes, followed by 750 nm. By measuring at, it is possible to confirm the total polyphenol content of the antioxidant component. A calibration curve was prepared using gallic acid as a standard for quantification, and the content of the total polyphenol compound in the sample was expressed in mg gallic acid equivalents (GE) / g). Shown.

As a result, the content of the total polyphenol compound of the main extract of fig leaf was measured in a concentration range of 0.1 to 1 mg / mL, showing a concentration-dependent tendency and a content corresponding to 5.0 to 46.7 mg / g (Fig. One). This was confirmed to be a total polyphenol content of about 4.7 to 5.0% when converted to an amount corresponding to gallic acid used as a standard.

In addition, the antioxidant activity has a scavenging activity of ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) free radicals of fig extract, preferably at a concentration of 0.1 to 1mg / mL In 3.8 to 5.2% ABTS is characterized by having a free radical scavenging activity.

In addition, the antioxidant activity is characterized by the free radical scavenging activity of DPPH (2,2-diphenyl-1-picrylhydrazyl) of 0.2 to 25.8 mg / g at a concentration of 0.1 to 1 mg / mL of the main extract of fig leaf.

DPPH and ABTS, which are chemically stabilized free radicals, dissipate electrons and change color when they react with antioxidants, so they are applied to experiments to apply DPPH and ABTS by three kinds of fig leaf solvent system fractions. The degree of color fading was measured and intended to be expressed as antioxidant power.

To measure antioxidant power using ABTS radicals, a 7.4 mM ABTS solution and 2.6 mM potassium bisulfite (K ₂ S ₂ O ₈ , potassium persulphate) solution were left in the cow for at least 12 hours to form a turquoise ABTS radical. , Diluted at 734 nm so that the absorbance value was 1.5 was used. Subsequently, 50 μL of the sample is added to the solution and left at room temperature for 60 minutes to measure the change in absorbance value of the reaction solution and ascorbic acid equivalent antioxidant for the standard ascorbic acid. capacity, AEAC).

To measure antioxidant activity using DPPH free radicals, 50 μL of a sample was added to a 0.2 mM DPPH radical solution and reacted at room temperature for 60 minutes to measure the change in absorbance of the reaction solution at 517 nm. The results were compared and calculated with ascorbic acid equivalent antioxidant capacity (AEAC) for ascorbic acid, a standard substance, similar to ABTS scavenging activity.

As shown in FIG. 2, the ABTS free radical scavenging activity of the main extract of fig leaf was measured in a concentration range of 0.1 to 1 mg / mL, showing a concentration-dependent tendency and showing an antioxidant power of about 3.8 to 5.2%. This corresponds to an antioxidant power of 38 to 52 mg / g when converted to an amount equivalent to ascorbic acid used as a standard.

On the other hand, as shown in Figure 3, in the antioxidant power using another type of radical DPPH, the activity of the main extract of Fig leaf showed a concentration-dependent tendency in the concentration range of 0.1 ~ 1 mg / mL. When converted to an equivalent amount of ascorbic acid used as a standard substance, it was found that the main extract of fig leaf had antioxidant activity of 0.2 to 25.8 mg / g.

As a result of ABTS free radical scavenging activity, the result showed the best DPPH free radical scavenging activity in the EtOAc layer (107.1 mg ascorbic acid equivalent / g; about 10.7% antioxidant power compared to ascorbic acid), H2O layer and BuOH layer Showed antioxidative power of 37.7 mg / g (3.8%) and 30.9 mg / g (3.1%) compared to ascorbic acid, respectively.

Through the results of the polyphenol content and the two types of radical scavenging activity, the main extract of fig leaf will be able to guess good results in the anti-inflammatory and whitening activity to be performed later.

The anti-inflammatory activity is characterized in that the IL-6 inhibitory activity is 25.3 to 32.5% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10-50 μg / ml of the main extract of fig leaf.

In addition, the anti-inflammatory activity is characterized in that the IL-12 inhibitory activity is 26.1 to 35.4% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10-50 μg / ml of the main extract of fig leaf.

To evaluate the anti-inflammatory activity, toxicity was evaluated for the RAW 264.7 cell line, a murine macrophage cell line.

The cell line was obtained from the Korean Cell Line Bank, and DMEM with 10% fetal bovine serum (FBS) and 1% penicillin / streptomycin (P / S) was added. The medium (Dulbecco's modified Eagle's medium) was subcultured once every 2-3 days under conditions of 37 ° C and 5% CO2 in a medium and used in the experiment.

First, in order to evaluate the toxicity of the sample to the cells, the cells were plated in a 96-well plate at 2 × 10 6 / well and incubated for 12 hours in a 37 ° C, 5% CO ₂ incubator to stabilize the cells. Thereafter, all the culture medium was removed, 160 µl of fresh medium and 20 µl of a suitably diluted sample were added and cultured for 30 minutes, followed by administration of lipopolysaccharide from Escherichia coli (LPS) extracted from E. coli as an inflammatory substance Cultured for an hour. After removing all the culture medium, 100 µl of EZ-cytox (Daeil Labservice) solution dissolved in PBS at a concentration of 10% was added to measure the absorbance at 450 nm to measure the cytotoxicity of the sample in the saline control. It was expressed in terms of cell viability (%).

The inflammatory response in the body is a defense function against risky stimuli such as microbial infection, endotoxin, and tissue damage. This is a reaction that is essential to the normal restoration of tissue structure and function. In normal inflammatory reactions, the production of pro-inflammatory mediators is reduced over time, and anti-inflammatory mediators are increased, so the inflammatory response is regulated by itself. Macrophages, one of the cells involved in the inflammatory response in the body, are activated by being exposed to stimuli such as LPS, which is a cell membrane component of Gram-negative bacteria, and play an important role in the inflammatory response. Activated macrophages are interferon-γ (IFN-γ), It is known to promote the production of inflammatory cytokines such as IL-1β, IL-6, IL-12 and TNF-α.

In addition, through the expression of enzymes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX) in addition to inflammatory cytokines, inflammatory mediator molecules such as nitric oxide (NO) and prostaglandin E2 (PGE2) are generated, and these mediators It is known that excessive production contributes to the development of various chronic inflammatory diseases. In order to examine the applicability as a health functional material capable of preventing or delaying the development of inflammatory diseases, research using the macrophage cell line RAW 264.7 has been actively conducted, and in the present invention, anti-aging using RAW 264.7 cell line against the main extract of fig leaf Examine the inflammatory activity.

Prior to the experiment, the sample was evaluated for toxicity to RAW 264.7 cells. As shown in FIG. 4A, the ethanol extract of fig leaves did not show cytotoxicity in the concentration range of 1 to 100 μg / mL, but when the concentration increased to 1,000 μg / mL, it exhibited about 55% cytotoxicity compared to the LPS treatment group. Was confirmed.

Therefore, the present invention selected a concentration range of 10-50 μg / mL as a sample concentration range in which cytotoxicity did not occur through re-testing, and then evaluated anti-inflammatory activity in a concentration range in which toxicity did not appear as shown in FIG. 4B.

As shown in Figure 5, the fig leaf extract did not show inhibitory activity of nitrogen oxide (nitric oxide, NO) induced by LPS in the range of 10 to 50 μg / mL, the concentration at which cytotoxicity did not appear (p <0.05). ).

In the measurement of the inhibitory capacity of inflammatory cytokines IL-6 and IL-12, the main extract of fig leaf showed a concentration-dependent inhibitory activity effect, and this tendency was statistically significant (Fig. 6).

In the case of IL-6 inhibitory activity (FIG. 6A), at a concentration of 10-50 μg / mL, the inhibitory activity was about 25.3-32.5% compared to the LPS-treated group, and all of them showed p <0.05 (when compared to the LPS-treated group) 95% confidence level).

IL-12 inhibitory activity (Fig. 6B) also showed a concentration-dependent inhibitory effect at a concentration of 10-50 μg / mL, which was found to have an inhibitory capacity of about 26.1 to 35.4% compared to the LPS-treated group. All were statistically significant in the range of p <0.01 (99% confidence level) compared to the LPS treatment group.

The whitening activity is characterized by inhibiting the expression of tyrosinase, and preferably the expression inhibitory activity of tyrosinase at a concentration of 0.265 to 2.5 mg / ml of fig leaf extract is 15.7 to 82.5%. It is characterized by being.

The method for measuring tyrosinase inhibitory activity is as follows.

 In a test tube, 220 μL of 0.1 M phosphate buffer (pH 7.0), 20 μL of sample solution, and 20 μL of tyrosinase from mushroom (12500 U / mL) are sequentially added. Here, 40 μL of 1.5 mM tyrosine solution was added as a substrate for tyrosinase, reacted at 37 ° C. for 10-15 minutes, and absorbance was measured at 490 nm. The sample solution was used instead of the sample solution, and the sample solution was corrected. As a positive control, kojic acid in a concentration range of 0 to 100 μg / mL was used. The tyrosinase activity inhibition rate by the sample was derived through the following equation (Fig. 7).

[Mathematics]

(a is the absorbance measured after the reaction of the test sample solution, b is the absorbance measured after the reaction of the sample solution, and a 'and b' are the absorbance measured by replacing the tyrosinase with a buffer solution)

The evaluation of whitening activity is evaluated by confirming the inhibitory activity of tyrosinase, which acts as a key enzyme in the synthesis of melanin, a major material for skin deposition and aging. That is, tyrosinase is an enzyme that is involved in the initial rate determining step, which is the most important step in the biosynthetic pathway of melanin in the human body, and inhibiting the activity of this enzyme inhibits melanogenesis.

This experiment is well known as a method of evaluating the effect of a test sample on inhibition of tyrosinase activity by reacting a test sample, purified tyrosinase and a substrate tyrosine in vitro (FIG. 7).

First, the sample was dissolved in distilled water step by step at a concentration of 0.625 to 10 mg / mL, and when the color change was observed, the main extract of fig leaf showed a visible yellow-based color at a concentration of 2.5 mg / mL or more. To evaluate the tyrosinase inhibitory activity, experiments were conducted in a concentration range of 0.625 to 2.5 mg / mL without color.

As shown in FIG. 8A, kojic acid used as a standard substance showed tyrosinase inhibitory activity of 3.9 to 84.8% in a concentration range of 0.1 to 0.4 mg / mL, while it was shown in FIG. 8B. As shown, the main extract of fig leaf showed 15.7 to 82.5% inhibitory activity in the concentration range of 0.625 to 2.5 mg / mL.

When calculated as a concentration capable of inhibiting 50% of tyrosinase activity based on the results of tyrosinase inhibitory activity by concentration (half maximal inhibitory concentration, IC50), the standard substance, nurukic acid, has an IC of 0.29 mg / mL. ₅₀ , and the ethanol extract of fig leaves showed an IC ₅₀ value of 1.52 mg / mL (FIG. 9).

This can inhibit 50% of tyrosinase activity with a small amount of 0.29 mg / mL in the case of nurukic acid, whereas the ethanol extract of fig leaf, which is a sample, requires a relatively large amount of 1.52 mg / mL to be applied to tyrosinase. It can be interpreted that it can inhibit 50% of the activity.

Nuruk acid is a substance obtained through fermentation of yeast used to make brewed food such as traditional alcohol, miso, and soy sauce, and through many studies, kojic acid is a tyrosinase, a melanin-producing enzyme that causes famine and freckles. It has been confirmed that it prevents freckles and freckles by inhibiting its activity, and is a functional single substance that has been widely recognized as one of the whitening ingredients with good efficacy.

In the present invention, it did not exhibit tyrosinase inhibitory activity as much as the well-known whitening ingredient, nurukic acid, but it clearly showed a concentration-dependent inhibitory effect in the simple extract state of fig leaf to whitening activity in the main extract of fig leaf. It was thought to contain the functional substances involved, which were thought to be polyphenol-based substances.

In the future, evaluation of fig leaves for intracellular melanin synthesis using melanocytes and cell lines will be conducted, and it is thought that structural identification of substances involved in various physiological activities can be performed simultaneously.

In the cosmetic composition of the present invention, an essential ingredient, the main extract of the fig leaf, and other ingredients, which are usually formulated in a cosmetic composition, can be blended as necessary. And inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavors, blood circulation accelerators, coolants, limiting agents, purified water, and the like.

In addition, according to an embodiment of the present invention, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence , Pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, and any one selected from the group consisting of powder powder provides a cosmetic composition containing the main extract of fig leaf do.

On the other hand, in the cosmetic composition comprising the main extract of fig leaves according to the present invention; A soap prepared by further comprising a herbal medicine, persimmon extract, evening primrose oil saponified product, sorghum powder extract, pig potato powder extract, cacao bean crushed product, ginseng extract, and mung bean extract is prepared, and the combination of each component It was confirmed that it has a higher synergistic effect than the simple sum.

Specifically, each component of fig leaf extract, herbal medicines (all of which are listed as herbal medicines), persimmon extract, evening primrose oil saponification, sorghum powder extract, pig potato powder extract, cacao bean crushed product, ginseng extract, and mung bean extract Soap using only raw materials (each control group) and soap (experimental group) combining all of the above components were prepared, and 20 groups of 20 to 50 adults in one group were grouped against 9 groups, and 9 control groups and experimental groups were prepared. It was distributed to each group and washed repeatedly three times a day for one week, and a questionnaire was conducted for changes for two weeks.

More specifically, the present invention, the fig leaf alcoholic extract is 5 to 15% by weight, preferably 10% by weight, the crude drug is 10 to 20% by weight, preferably 15% by weight, the persimmon extract is 5 to 10% by weight %, Preferably 7% by weight, the evening primrose oil saponification is 5 to 15% by weight, preferably 10% by weight, the sorghum powder extract is 0.5 to 5.0% by weight, preferably 2.5% by weight, the pig potato powder The extract is 0.5 to 5.0% by weight, preferably 2.5% by weight, and the cacao pulverized product is 1 to 10% by weight. Preferably 5% by weight, the mung bean extract containing bitexin and isobitexin is 8 to 12% by weight, preferably 10% by weight.

Therefore, the experimental group is 10% by weight of the main extract of the fig leaf, 15% by weight of the herbal medicine, 7% by weight of the persimmon extract, 10% by weight of the evening primrose oil saponification, 2.5% by weight of the sorghum powder extract, 2.5% of the pig potato powder extract %, 5% by weight of the cacao beans, 10% by weight of the mung bean extract, and soap prepared with soap paste or the like is used.

On the other hand, the control group has the same weight percent as the experimental group, and uses the soap prepared by using the rest as a soap paste.

As a result, the results of the survey of the first group using the experimental group and the soap (control group) made only from the extract of the main extract of the fig leaf, significantly improved in the experimental group compared to the control group (10 persons), slight improvement It was counted as (6) and no change (4).

As a result of a survey of the second group using the test group and soaps made of only the raw drug (all of which were previously listed as herbal drugs) as a sole raw material, significantly improved in the experimental group compared to the control group (9 persons), slightly It was counted as improvement (5 people) and no change (4 people).

As a result of the questionnaire survey on the third group using the experimental group and the soap (control group) made only of persimmon extract, significantly improved (7 persons), slight improvement (5 persons) in the experimental group compared to the control group, It was counted as no change (8 people).

As a result of the questionnaire survey on the fourth group using the experimental group and the soap (control group) made of only the evening primrose oil saponified product, significant improvement (7 persons) and slight improvement (5 persons) compared to the control group ), No change (8).

As a result of the questionnaire survey on the fifth group using the experimental group and the soap (control group) made only from the extract of sorghum powder, significantly improved (6 persons) and slight improvement (6 persons) in the experimental group compared to the control group , No change (8).

As a result of the questionnaire survey on the 6th group using the experimental group and the soap (control group) prepared only with the extract of the pig potato powder, significantly improved (5 persons), slight improvement (5 persons) compared to the control group ), And there was no change (10).

As a result of the questionnaire survey on the 7th group using the experimental group and the soap (control group) prepared only from the cocoa beans crushed material, significantly improved (6 persons) and slight improvement (3 persons) compared to the control group ), And there was no change (11).

As a result of the questionnaire survey on the 8th group using the experimental group and the soap (control group) made only from the ginseng extract alone, significantly improved (6 persons) and slight improvement (5 persons) in the experimental group compared to the control group , No change (11).

As a result of the questionnaire survey on the ninth group using the experimental group and the soap (control group) made only of the mung bean extract, significantly improved (7 persons), slight improvement (7 persons) in the experimental group compared to the control group, It was counted as no change (6).

As a result of this survey, in the cosmetic composition comprising the main extract of fig leaf; Further containing herbal medicines, persimmon extract, evening primrose oil saponification, sorghum powder extract, pig potato powder extract, cacao bean crushed products, ginseng extract, and mung bean extract have significant improvement compared to those used alone as one of them. Was confirmed.

Claims (9)

In the cosmetic composition comprising the main extract of fig leaf;
Herbal medicine, persimmon extract, evening primrose oil saponification, sorghum powder extract, pig potato powder extract, cacao bean pulverized, ginseng extract and mung bean extract further comprising;
The fig leaf extract has antioxidant, anti-inflammatory and whitening activity;
The herbal medicine is a fine powder of hangin, Yulmu, Yulpi, Doin, Baekgangjam, sedija, peppermint, Baeknyeong, Baekji and wormwood;
The persimmon extract is extracted from persimmon skin and nipple, and is obtained by using a distillation method;
The evening primrose oil saponification is saponified by treating the evening primrose oil with any alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide and potassium hydroxide;
The water powder extract is 0.5 to 5.0% by weight;
The pork potato powder extract is 0.5 to 5.0% by weight;
The cacao bean pulverized product is 1 to 10% by weight of 48 to 72 hours of continuous pulverization with no alkali treatment;
The ginseng extract refers to a fermented ginseng to increase a specific ginseng saponin substrate;
The mung bean extract is obtained by immersing the mung bean powder in an aqueous alcohol solution and contains bitexin and isobitexin as active ingredients; a cosmetic composition comprising a main extract of fig leaf. According to claim 1,
The fig leaf extract is a cosmetic composition comprising a fig leaf extract, characterized in that it has antioxidant, anti-inflammatory and whitening activity. According to claim 2,
The antioxidant activity is a cosmetic composition comprising a fig leaf extract, characterized in that the polyphenol of 5.0 to 46.7mg / g at a concentration of 0.1 to 1mg / mL of the extract of the fig leaf. According to claim 2,
The antioxidant activity is a cosmetic composition comprising a fig leaf alcoholic extract characterized by ABTS free radical scavenging activity of 3.8 to 5.2% at a concentration of 0.1 to 1 mg / mL of the fig leaf alcoholic extract. According to claim 2,
The antioxidant activity is a cosmetic composition comprising a fig leaf alcohol extract characterized by DPPH free radical scavenging activity of 0.2 to 25.8 mg / g at a concentration of 0.1 to 1 mg / mL of fig leaf alcoholic extract. According to claim 2,
The anti-inflammatory activity includes a fig leaf alcoholic extract characterized in that the IL-6 inhibitory activity is 25.3 to 32.5% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10-50 μg / ml of the fig leaf alcoholic extract. Cosmetic composition to be said. According to claim 2,
The anti-inflammatory activity includes a fig leaf alcoholic extract characterized in that the IL-12 inhibitory activity is 26.1 to 35.4% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10-50 μg / ml of the fig leaf alcoholic extract. Cosmetic composition to be said. According to claim 2,
The whitening activity is a cosmetic composition comprising a fig leaf extract, characterized in that the activity of inhibiting the expression of tyrosinase (tyrosinase) at a concentration of 0.265 to 2.5mg / ml of the fig leaf extract is 15.7 to 82.5%. Skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing A cosmetic composition comprising any of the fig leaf extracts of any one of claims 1 to 8 as a formulation selected from the group consisting of lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders and rust powders.

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