KR101978838B1 - Multi-spectral autoanalysis endoscope apparatus and method of processing image using the same - Google Patents

Abstract

The present invention relates to a multi-wavelength endoscopic system for photographing an observation site marked with a plurality of fluorescent materials having different colors, the system comprising: polarizing incident light reflected at a viewing site in a first direction and a second direction perpendicular to the first direction An image pickup unit for dividing a spectral region of the incident light polarized in the first direction and the second direction into a plurality of spectral channels and measuring image intensity of each of the plurality of spectral channels, Storing a single fluorescence spectrum extracted from the sample image data obtained by singly processing the observation region with each of the fluorescent materials, and storing the image data photographed by the photographing unit in the single fluorescent spectrum using one of the plurality of fluorescent substances And the fluorescence material of the fluorescent material is displayed separately.

Description

TECHNICAL FIELD [0001] The present invention relates to a multi-wavelength endoscopic system and an image processing method using the same. [0002]

The present invention relates to a multi-wavelength endoscopic system and an image processing method using the same.

Still, the incidence of cancer continues at a high rate, and if a diagnosis is made through an endoscope, there is a possibility of misdiagnosis because the tumor must be detected visually.

In particular, if the shape of the polyp is flat rather than lumpy, the probability of detection is lower.

Recently, with the development of molecular imaging technology, there have been ongoing studies to diagnose gastrointestinal cancer using this technique, or to image the molecular characteristics of cancer. The first attempt to introduce a molecular image targeting Cathepsin B to endoscopy was simple, but the images at that time were very simple and did not reach clinically applicable levels.

Recently, various studies have been carried out by various organizations. Until recently, techniques for imaging a specific tumor tissue using a peptide as a probe, ultra-small imaging technology capable of high-speed 3-dimensional endoscope imaging, and small microscope technology have been developed. A more advanced marker material is being developed through the development of image-capable Raman amplification probes and compact-probe-based compact fluorescent probes.

Dr. of the University of Mainz in Germany. The Goetz group has developed a probe that can identify the Epidermal Growth Factor Receptor (EGFR), and has attempted to image it using a special endoscope called Confocal Endomicroscopy.

However, until now, research has been continuing to make endoscopy possible using molecular imaging, but researches that have obtained a level of image applicable to actual endoscopes are rare, and even in the case of a probe which is very important in molecular imaging, It only shows the level of technology that verifies and verifies the probe.

SUMMARY OF THE INVENTION The present invention provides a multi-wavelength endoscopic system capable of processing image data of a marked observation region using a plurality of probes for a complex target and diagnosing diseases, and an image processing method using the same will be.

A multi-wavelength endoscope system for photographing an observation region marked with a plurality of fluorescent materials having different colors according to an embodiment of the present invention includes a first wavelength selective member for selectively irradiating incident light reflected at an observation site in a first direction and a second An image pickup unit for dividing a spectral region polarized in the first direction and the second direction into a plurality of spectral channels and measuring intensity of each of the plurality of spectral channels to obtain image data; Storing a single fluorescence spectrum extracted from the sample image data obtained by photographing the observed region where each of the plurality of fluorescent substances has been treated as a single one and storing the image data photographed by the photographing unit using the single fluorescent spectrum among the plurality of fluorescent substances And a computing unit for separating and outputting only one fluorescent substance so as to be displayed respectively.

Wherein the photographing unit comprises: a beam splitter for polarizing the incident light in the first direction and the second direction perpendicular to the first direction; a first beam splitter disposed in a path of light separated in the first direction, A first area camera located on the path of the light separated in the second direction and passing through the set range of light, a first area camera measuring the intensity of the light passing through the first area filter, And a second area camera that measures the intensity of light passing through the two-region filter.

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The computing unit may store the unprocessed fluorescence spectrum extracted from the raw image data obtained by photographing the observation region not treated with the fluorescent material.

The computing unit may perform correction to remove the self fluorescent component included in the image data photographed by the photographing unit using the unprocessed fluorescence spectrum.

A multi-wavelength endoscope system for photographing an observation site marked with a plurality of fluorescent substances having different colors according to an embodiment of the present invention, the system comprising: A first area filter positioned in a path of light separated in the first direction and passing light in a set range, a second area filter positioned in a path of light separated in the second direction, A first area camera for measuring the intensity of light passing through the first area filter, a second area camera for measuring the intensity of light passing through the second area filter, The image data obtained by using the intensity of the light having passed through the area filter and the intensity of the light having passed through the second area filter, And a computing unit for separating and outputting only the substance to be displayed.

Wherein the computing unit stores a single fluorescence spectrum extracted from the sample image data obtained by photographing the observation region obtained by singly processing the plurality of fluorescent materials and transmits the image data photographed by the photographing unit to the plurality of Only one fluorescent substance of the fluorescent substance can be separated so as to be respectively displayed.

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The computing unit may store the unprocessed fluorescence spectrum extracted from the raw image data obtained by photographing the observation region not treated with the fluorescent material.

A method for processing an image in a multi-wavelength endoscopic system according to an embodiment of the present invention includes the steps of irradiating a light source to an observation site marked with a plurality of fluorescent materials having different colors, Separating the image data so that only one fluorescent substance of the plurality of fluorescent substances is displayed, and outputting the separated image data according to the wavelength band.

The image processing method may further include a step of extracting a single fluorescence spectrum from the image data obtained by photographing the observation region where each of the plurality of fluorescent substances has been uniquely processed.

Separating the image data so that only one fluorescent substance of the plurality of fluorescent substances is displayed, the image data is separated using only the single fluorescent spectrum so that only one fluorescent substance of the plurality of fluorescent substances is displayed .

The image processing method may further include extracting an untreated fluorescence spectrum from unprocessed image data obtained by photographing an observation site not treated with the fluorescent material.

The image processing method may further include performing correction to remove the self fluorescent component included in the image data using the unprocessed fluorescence spectrum.

In the multi-wavelength endoscopic system according to the embodiment of the present invention, the labeled observation sites are separated and output according to a predetermined wavelength band using a plurality of probes, so that the disease occurrence site can be precisely identified.

In the multi-wavelength endoscopic system according to the embodiment of the present invention, the self-fluorescence component included in the image data of the observed region is removed, and the false positive error can be reduced, thereby reducing the possibility of misdiagnosis.

1 is a block diagram of an automatic multi-wavelength analyzing system according to an embodiment of the present invention.
2 is a diagram illustrating an exemplary structure of a photographing unit according to an embodiment of the present invention.
3 is a view illustrating an example of a photographing unit according to an embodiment of the present invention.
4 is a diagram illustrating a result of driving a variable liquid crystal filter according to an embodiment of the present invention.
5 is a flowchart illustrating an image processing method using a multi-wavelength automatic analysis endoscopic system according to an embodiment of the present invention.
6A to 6D are graphs illustrating a simulation apparatus for evaluating the performance of the multi-wavelength automatic analysis endoscopic system according to an embodiment of the present invention, and simulation results thereof.
FIG. 7 is a diagram showing image results obtained from an untreated tissue sample and a single fluorescence-treated tissue sample by the multi-wavelength automated endoscopic system according to an embodiment of the present invention.
FIG. 8 is a view showing a result of an endoscopic imaging of a tissue sample treated with a plurality of fluorescent materials using a multi-wavelength automatic analysis endoscopic system according to an embodiment of the present invention.
9 is a view showing an endoscopic imaging result of a living colon cancer model mouse using a multi-wavelength automatic analysis endoscopic system according to an embodiment of the present invention.
FIG. 10 is a view showing an endoscopic imaging result of a live colon cancer model pig using a multi-wavelength autoanalysis endoscopic system according to an embodiment of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, which will be readily apparent to those skilled in the art to which the present invention pertains. However, the present invention can be implemented in various different forms, and is not limited to the embodiments described herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and like parts are denoted by similar reference numerals throughout the specification.

Throughout the specification, when an element is referred to as " comprising ", it means that it can include other elements as well, without excluding other elements unless specifically stated otherwise. Also, the terms " part, " " module, " and " module ", etc. in the specification mean a unit for processing at least one function or operation and may be implemented by hardware or software or a combination of hardware and software have.

Hereinafter, an automatic multi-wavelength analyzing system according to an embodiment of the present invention will be described in detail with reference to the drawings.

FIG. 1 is a configuration diagram of an automatic multi-wavelength automatic analysis system according to an embodiment of the present invention. FIG. 2 is a diagram illustrating a structure of a photographing unit according to an exemplary embodiment of the present invention. 1 is a diagram illustrating an example of a photographing unit according to an embodiment.

Referring to FIG. 1, the multi-wavelength endoscopic system 100 is a system using a camera that allows an object to be detected to be observed with the naked eye and a camera to which a multi-fluorescence imaging is enabled so that the visible light region is continuously photographed Obtain the supersensitized radiance of the channel.

Here, the channel is a unit band for measuring wavelength, and the spectral image of each channel can be obtained by adjusting the filter.
In the present specification, an endoscope is a word that goes through a machine that observes the inside of the body, and includes a bronchoscope, a gastroscope, a hard shaft, and an anus.

The multi-wavelength endoscope system 100 includes a photographing unit 200 and a computing unit 300.

The photographing unit 200 includes an objective lens 210, a relay lens 220, a beam splitter 230, a first area lens 240, a first area filter 242, a first area camera 244, Area lens 250, a second area filter 252, and a second area camera 254.

The light source 400 shown in FIG. 2 located outside the multi-wavelength endoscope system 100 irradiates light to excite a photographed region. The light source 400 may include two or more light sources having different wavelengths in order to capture an object to be observed marked with a fluorescent sample having different wavelengths.

In this embodiment, the subject to be observed is a marker that is expressed in cancer. In this embodiment, the marker can be made into a probe having various wavelength ranges and labeled using different fluorescent dyes.

The objective lens 210 is a lens through which incident light enters. The objective lens 210 may provide an image focused at any wavelength in the spectral region of the multi-wavelength endoscope system 100.

The relay lens 220 is a lens for advancing incident light along the optical axis, and outputs light in parallel. The relay lens 220 may be a triplet lens having a predetermined focal length.

Referring to FIG. 2, the relay lens 220 according to the present embodiment may be connected to a light source 400 that emits light for exciting a photographed region. The relay lens 220 may be located inside the endoscope inserted into the body for photographing the subject to be examined.

The beam splitter 230 separates the parallel light in two directions. The beam splitter 230 is a polarization-based beam splitter that processes incident light in the wide-band spectrum. The beam splitter 230 may cover the spectrum within the visible light region.

Light composed of electric fields in various directions is polarized in two directions called p-polarization and s-polarization. Here, the p-polarized light means parallel to the slit direction of the polarizing plate, and the s-polarized light means perpendicular to the slit direction of the polarizing plate.

The first zone lens 240 and the second zone lens 250 are located in the path of light divided from the beam splitter 230, respectively. The first area lens 240 and the second area lens 250 are disposed perpendicular to each other to adjust the light split from the beam splitter 230 to a predetermined magnification, Can be obtained.

The first area lens 240 and the second area lens 250 adjust the light split from the beam splitter 230 at an appropriate magnification and transmit the adjusted light to the first area filter 242 and the second area filter 252 ). ≪ / RTI >

The first area filter 242 and the second area filter 252 can pass light in a specified spectral range among the light passing through the first area lens 240 and the second area lens 250, respectively.

The first region filter 242 and the second region filter 252 may be, for example, a Liquid Crystal Tunable Filter (LCTF), which is a local bandpass filter for passing light in a designated spectral region.

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As a filter of the present system, when a variable liquid crystal filter that passes a channel in a specific wavelength band in a spectral range (for example, 440 nm to 720 nm) is used, it can be controlled to pass light at 10 nm intervals, for example .

Since the variable liquid crystal filter can electronically change the wavelength, wavelength selection is possible at a high speed.

Referring to FIG. 4, the variable liquid crystal filter (www.perkinelmer.co.kr) can be controlled to pass light at predetermined intervals, and has the effect of putting several tens to several hundreds of filters into one filter, Multi-wavelength imaging can be implemented in sharp, diverse colors.

1, the first area camera 244 and the second area camera 254 are disposed at the end of the optical path, respectively, and pass through the first area filter 242 and the second area filter 252 Measure the intensity of light.

The first area camera 244 and the second area camera 254 may be monochrome cameras. At this time, the first area camera 244 and the second area camera 254 can acquire the intensity of the focused image through a positive triplet lens having a predetermined focal distance, respectively.

The computing unit 300 may sort spectral images of the channels obtained by the photographing unit 200 and output the radiance depending on the wavelengths. The multi-spectral image may be composed of a combination of spectral images photographed for a plurality of channels.

The computing unit 300 separates the multi-spectral image taken by the photographing unit 200 according to the wavelength band and outputs the separated multi-spectral image.

In this embodiment, in order to accurately detect various markers related to the disease at the site to be photographed and accurately diagnose the disease, the site to be photographed can be labeled with fluorescent materials of different wavelengths.

In the case of labeling a single marker with a single fluorescent material, it is difficult to accurately determine the disease site. In this embodiment, the complex probe is labeled with fluorescent materials of different wavelengths, Can be imaged.

The spectroscopic image obtained by photographing the photographed region marked with the fluorescent material having different wavelengths by the photographing unit 200 may display a fluorescent signal having different wavelength regions.

When a plurality of markers labeled with fluorescent materials having different wavelengths are used to photograph a region to be observed, a plurality of fluorescent materials may interfere with the image, and it may be difficult to distinguish each fluorescent material.

In addition, a substance other than a marker labeled with a fluorescent substance may be irradiated with the excitation light irradiated from the light source 400 and emit intrinsic light.

For example, the autofluorescence phenomenon in which collagen, elastin, keratin, NADH, flavin, porphyrin, etc. contained in the biological tissue to be observed is reflected by excitation light Lt; / RTI >

There is a possibility of misdiagnosis when diagnosing the disease by using the result of photographing in which the autofluorescent material, which is generally distributed in the body rather than the labeling substance to be detected due to the self fluorescence phenomenon, is mistaken for the marker.

Accordingly, the computing unit 300 of the multi-wavelength endoscopic system according to an exemplary embodiment of the present invention allows the user to precisely diagnose a multi-spectral image, So that the fluorescence can be separated and outputted according to the wavelength band of each fluorescent material.

At this time, the computing unit 300 can extract a self fluorescence spectral result indicating the intensity of light according to a wavelength band from a self-fluorescence image obtained by photographing a non-treat tissue sample.

Also, the computing unit 300 may extract a single fluorescence spectral result indicating the intensity of light according to the wavelength band from a plurality of single processed image images obtained by photographing a tissue sample of a single Treated single fluorescent material.

First, the computing unit 300 extracts an image spectrum result indicating the intensity of light according to a wavelength band in the image data photographed by the photographing unit 200, and calculates the intensity of light according to each wavelength band according to the spectrum of the self- And corrects to delete the self fluorescent portion.

The computing unit 300 may calculate a normalized value representing the intensity of the light according to the wavelength band from the single fluorescence spectrum. For example, it is possible to calculate the ratio of the intensity of light according to each wavelength band by setting the intensity of light according to the propagation field band to 100. [

Next, the computing unit 300 unmixes the image spectral results of the image data by the intensity of the light according to each wavelength band according to the normalization value calculated from the single fluorescence spectrum, The photographed image may be divided into a plurality of image images so that only the respective fluorescent materials are displayed.

Accordingly, the computing unit 300 according to an exemplary embodiment of the present invention performs correction to remove the autofluorescence component from the multispectral image in order to reduce the probability of misdiagnosis when diagnosing disease or not according to the photographing result, Only a marked marker can be displayed.

In addition, by separating the image of the observed region marked with a plurality of markers so that only the respective markers are displayed, it is possible to accurately diagnose the division of the cancer by complementing different markers.

5 is a flowchart illustrating an image processing method using the multi-wavelength automatic analysis endoscopic system according to an embodiment of the present invention.

Referring to FIG. 5, a region to be photographed is labeled with a fluorescent material in various wavelength regions (S110). In this experiment, the site to be photographed may be internal tissues for cancer screening, and is a marker that is expressed in cancer. In this embodiment, the marker can be labeled using a probe labeled with a fluorescent material in various wavelength ranges .

Table 1 shows the various area probes for multiple wavelength detection.

Probe Name Marker Labeling substance Wavelength region (nm) HMRG g-Glutamyl transpeptidase Rhodamine 501/524 Cetuximab EGFR receptor Flamma553 553/570 Herceptin Her-2 receptor Flamma675 675/700

The probe may be an antibody probe. Antibody probes in this example can be EctR and Cetuximab and Herceptin, which are targeted antibodies to EGFR and HER2, which are highly expressed in tumor and colorectal cancer cells. In this example, Cetuximab and Herceptin were labeled with Flamma-553 and Flamma-675 fluorescent materials, respectively.

Further, the probe may be an active probe. In this embodiment, the active probe may be gGlu-HMRG, which exhibits fluorescence activity in association with tumor cells and GGT (gamma-glutamyltranspeptidase), which is highly expressed in colon cancer cells. In this embodiment, HMRG may be labeled with rhodamine.

Antibody probes may be administered intravenously to the tail of a mouse 48 hours prior to multi-wavelength detection endoscopic image acquisition, and active probes may be applied to the colon 10 minutes before the multi-wavelength detection endoscope is performed.

Then, the excitation light source is irradiated to the photographed region, and the photographed image is acquired by receiving the reflected light (S120).

At this time, the light source entered through the endoscope end excites the observation object, and the light reflected from the observation object is transmitted to the first area camera 244 and the second area camera 254 through the relay lens 220 .

The light source 400 may include two or more light sources having different wavelengths in order to capture an object to be observed marked with a fluorescent sample having different wavelengths.

The first region camera 244 and the second region camera 254 each include a first region filter 242 and a second region filter 252 and are each a local bandpass filter that passes light in a specified spectral region, Liquid crystal filter.

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The self-fluorescence portion included in the next captured image is removed, separated according to a predetermined wavelength band, and output (S130).

The fluorescence spectral data obtained through the endoscope outputs the result divided by the wavelength band through the division operation of the computing unit 300, and the autofluorescence portion is removed to finally acquire the image of the wavelength region to be obtained from the observation target .

The multi-wavelength endoscope system 100 may store a self fluorescence spectrum and a single fluorescence spectral result, which represent intensity of light according to a wavelength band of a pre-stored untreated tissue sample image and a single-processed tissue sample image.

At this time, it is possible to further store the normalization value indicating the intensity of light according to the wavelength band calculated from the self fluorescence spectrum and the single fluorescence spectrum.

The multi-wavelength endoscope system 100 then corrects for eliminating the autofluorescence portion by attenuating the spectrum of the image data photographed with a plurality of fluorescent substance-labeled observation regions by the autofluorescence spectrum normalized value.

Then, the multi-wavelength endoscope system 100 separates the image data of the image data by unmixing the intensity of the light according to each wavelength band according to the normalized value calculated from the single fluorescence spectrum, It is possible to display a plurality of image images so that only the respective fluorescent materials appear.

That is, in the case where one marker is labeled with one fluorescence substance, it is difficult to accurately determine the disease site. Therefore, in this embodiment, by labeling the diseased site using a complex probe labeled with a fluorescent substance of different wavelength, Other probes can be supplemented to accurately image diseased areas.

In addition, the computing unit 300 according to an exemplary embodiment of the present invention performs correction to remove the autofluorescence component from the multispectral image to reduce the probability of misdiagnosis when diagnosing disease or not according to the photographing result, Only one marker can be displayed.

6A to 6C are graphs illustrating a simulation apparatus for evaluating performance of a multi-wavelength automatic analysis endoscopic system according to an embodiment of the present invention and simulation results thereof.

6A, a polyethylene tube (0.28 mm inner diameter, PE-10) having a length of about 15 mm was prepared to evaluate the performance of the multi-wavelength automatic analysis endoscope system according to an embodiment of the present invention. Fluorescent dye was injected.

One end of the tube was attached to a circular metal ring and the other end was collected toward the center of the endoscopic observation. The fluorescent dyes used used a plurality of dyes having different colors of visible light band and contained wavelength regions close to each other.

Referring to FIG. 6B, the tubes injecting the fluorescent dye were respectively photographed to acquire image data, and a spectrum representing the intensity of light according to the wavelength band was obtained from the respective image data results as shown in FIG. 6C. As a result, it is possible to identify regions separated by wavelengths of each dye.

At this time, the set wavelength range read in the multi-wavelength endoscope system 100 according to the present embodiment may be 420 nm to 620 nm.

Referring to FIG. 6D, a complete image image file is obtained through a decomposition process according to a single fluorescence spectrum result through the computing unit 300.

FIG. 7 is a view showing image results obtained from an untreated tissue sample and a single fluorescence-treated tissue sample by the multi-wavelength automatic analysis endoscopic system according to an embodiment of the present invention, and FIG. FIG. 2 is a view showing a result of endoscopic imaging of a tissue sample treated with a plurality of fluorescent materials using an automatic multi-wavelength analysis endoscopic system according to the present invention.

At this time, an active probe (HMRG) was injected via topical application, and an antibody probe (Cetuximab-Flamma553, Herceptin-Flamma675) was injected intravenously, and then colon tissues were extracted and analyzed using a multi-wavelength automatic analysis endoscope system Images of each wavelength were acquired.

Referring to FIG. 7, fluorescence images by autofluorescence can be observed in the non-treat colonic tissue of the non-probe-treated animal for the control experiment.

A single probe-treated tissue sample was taken at the observation site, and a single fluorescence spectral result indicating the intensity of light according to the wavelength band was extracted from the captured image data.

Referring to FIG. 8, in this embodiment, a composite probe labeled with a fluorescent material having a different wavelength is used. In the image data obtained by photographing a tissue sample labeled with a complex probe, only the fluorescent material is labeled according to a single fluorescence spectral result By separating and outputting the images, it can be confirmed that the images are imaged so as to accurately diagnose diseased parts by complementing different probes.

9 is a view showing an endoscopic imaging result of a living colon cancer model mouse using a multi-wavelength automatic analysis endoscopic system according to an embodiment of the present invention.

An active probe (HMRG) was injected into a colorectal mouse model mouse via topical application, and an antibody probe (Cetuximab-Flamma553, Herceptin-Flamma675) was injected intravenously, and then the multi-wavelength automatic analysis endoscope system And images of each fluorescent substance were obtained through colonoscopy.

While it is difficult to accurately determine each of the single probes by imaging the sections of the cancer to different regions, the composite probes complement the different probes and observe imaging the sections of the cancer.

FIG. 10 is a view showing an endoscopic imaging result of a living colon cancer model pig using a multi-wavelength autoanalysis endoscopic system according to an embodiment of the present invention.

An active probe (HMRG) and an antibody probe (Cetuximab-Flamma553, Herceptin-Flamma675) were injected into a human-like pig, and then an image of each wavelength was acquired through a colonoscopic endoscope system.

Fluorescence images were not obtained when the probe was not treated for control experiments.

Signals were detected only at the spectral wavelength of the fluorescent light labeled by each probe when each fluorescence probe was treated as a single treatment, and a triple-treated image of three probes was simultaneously processed using a single fluorescence spectral result Data were separated from the data so that only each fluorescent substance was labeled.

Thus, by complementing the different probes, it can be seen that false positive errors are reduced and the compartments of the cancer are imaged more accurately.

The embodiments of the present invention described above are not implemented only by the apparatus and method, but may be implemented through a program for realizing the function corresponding to the configuration of the embodiment of the present invention or a recording medium on which the program is recorded.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, It belongs to the scope of right.

Claims (15)

A multi-wavelength endoscope system for imaging an observation site marked with a plurality of fluorescent substances having different colors,
Polarized incident light reflected from the observation region in a first direction and a second direction perpendicular to the first direction and separating the spectral region of the incident light polarized in the first direction and the second direction into a plurality of spectral channels An imaging unit for acquiring image data by measuring light intensity of each of the plurality of spectral channels;
Storing the single fluorescence spectrum extracted from the sample image data photographed by treating the observation region with each fluorescent substance,
And a computing unit for separating and outputting the image data photographed by the photographing unit using the single fluorescence spectrum so that only one of the plurality of fluorescent materials is displayed,
The computing unit
Storing the untreated fluorescence spectrum extracted from the raw image data obtained by photographing the observation site not treated with the fluorescent substance,
And performing correction to remove the self fluorescent component included in the image data photographed by the photographing unit using the unprocessed fluorescence spectrum
Multi - Wavelength Endoscopy System. The method of claim 1,
The photographing unit
A beam splitter for polarizing the incident light in the first direction and the second direction perpendicular to the first direction;
A first area filter positioned in a path of light separated in the first direction and passing light in a predetermined range;
A second area filter located in a path of light separated in the second direction and passing light in a predetermined range,
A first area camera for measuring the intensity of light passing through the first area filter,
And a second region camera for measuring the intensity of light passing through the second region filter. delete delete A multi-wavelength endoscope system for imaging an observation site marked with a plurality of fluorescent substances having different colors,
A beam splitter for polarizing the incident light reflected from the observation region in a first direction and a second direction perpendicular to the first direction;
A first area filter positioned in a path of light separated in the first direction and passing light in a predetermined range;
A second area filter located in a path of light separated in the second direction and passing light in a predetermined range,
A first area camera for measuring the intensity of light passing through the first area filter,
A second area camera for measuring intensity of light passing through the second area filter,
Wherein the image data obtained by using the intensity of light passing through the first area filter and the intensity of light passing through the second area filter is separated and output so that only one of the plurality of fluorescent materials is displayed, ≪ / RTI >
The computing unit
Storing the untreated fluorescence spectrum extracted from the raw image data obtained by photographing the observation site not treated with the fluorescent substance,
And performing correction to remove the self fluorescence component included in the image data using the unprocessed fluorescence spectrum
Multi - Wavelength Endoscopy System. The method of claim 5,
The computing unit
Storing the single fluorescence spectrum extracted from the sample image data photographed by treating the observation region with each fluorescent substance,
And separating the image data so that only one of the plurality of fluorescent materials is displayed using the single fluorescence spectrum. delete delete A method for processing an image in a multi-wavelength endoscope system including a photographing unit and a computing unit,
Irradiating a light source to an observation site marked with a plurality of fluorescent substances having different colors by the photographing unit;
Acquiring image data by receiving light reflected from the observation site by the photographing unit;
Separating the image data so that only one of the plurality of fluorescent materials is displayed by the computing unit;
Outputting the separated image data according to a wavelength band by the computing unit;
The computing unit extracts the unprocessed fluorescence spectrum from the unprocessed image data photographed at the observation site not treated with the fluorescent material and performs a correction for removing the self fluorescence component contained in the image data using the unprocessed fluorescence spectrum The method comprising the steps of: The method of claim 9,
And separating the image data so that only one fluorescent material among the plurality of fluorescent materials is displayed,
And extracting a single fluorescence spectrum from the sample image data photographed by singly processing the observation region with each of the fluorescent materials. 11. The method of claim 10,
And separating the image data so that only one fluorescent material among the plurality of fluorescent materials is displayed,
And separating the image data so that only one fluorescent material of the plurality of fluorescent materials is displayed using the single fluorescence spectrum. delete delete delete delete

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