KR101971633B1 - Mixer device for gene discrimination chip and buffer and gene mixing method using it - Google Patents

Abstract

The present invention relates to a mixer device for gene discrimination chip, a PCR buffer and a gene mixing method using the same, and more particularly, to a mixer device for gene discrimination chip for efficiently mixing PCR buffer and gene, . The present invention is characterized in that a mixer main chamber part in which a PCR buffer is accommodated and mixed with a gene extracted from a bacteria to be detected; And a mixer auxiliary chamber portion connected to the mixer main chamber portion and adapted to receive the gene and the PCR buffer accommodated in the mixer main chamber portion, wherein the gene and the PCR buffer are connected to the mixer auxiliary chamber portion And the mixer main chamber portion is arranged to be mixed while reciprocating the mixer main chamber portion.

Description

TECHNICAL FIELD [0001] The present invention relates to a mixer apparatus for a gene discrimination chip, and a PCR buffer and a gene mixing method using the same.

The present invention relates to a mixer device for gene discrimination chip, a PCR buffer and a gene mixing method using the same, and more particularly, to a mixer device for gene discrimination chip for efficiently mixing PCR buffer and gene, .

In recent years, studies on nano-biotechnology capable of directly confirming and manipulating biomolecule behavior of nano units such as genes, proteins and cells of an object have been actively conducted. Such nano-biotechnology can be used to diagnose a disease or to determine the type of a target object, and thus the recent industrial field is being expanded.

Particularly, in recent years, the development of a lab-on-a-chip has been actively carried out. A lab-on-a-chip is a type of biochip that allows a small chip to perform research that can be done in a laboratory. Specifically, the lab-on-a-chip is made of plastic, glass, silicon, etc. to create microchannels of nanometer (nm) or less. Through this, labs and researches that can be done in existing laboratories It is a chip made to replace. Because of this practicality, lab-on-a-chip is continuously being developed and used.

However, since the conventional gene discrimination apparatus is large, it is inconvenient to carry and it takes a long time to discriminate the gene, which is a problem in that it is not practical.

In general, a lab-on-a-chip mixes a PCR buffer and a gene to amplify the gene. However, since the lab-on-a-chip is provided by laminating thin films, there is a problem that the PCR buffer injected between the thin films and the gene are difficult to be mixed quickly.

In addition, conventionally, when a mixed PCR solution containing a PCR buffer and a gene is injected into a lab-on-a-chip, a predetermined amount of human is injected into the lab-on-a-chip. Therefore, conventionally, it takes a lot of time to inject the mixed PCR solution into the lab-on-a-chip, and it is difficult to always inject a certain amount accurately, which lowers the reliability of the experiment.

Therefore, there is a need for a mixer device and a quantitative injection device for gene discrimination chip in order to efficiently mix PCR buffer and gene, and to automatically inject a constant amount of mixed PCR solution.

Korean Patent Registration No. 10-1421098 (Apr. 14, 2014)

It is an object of the present invention to solve the above problems by providing a mixer device for a gene discrimination chip for efficiently mixing a PCR buffer and a gene, and a PCR buffer and a gene mixing method using the same.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

According to an aspect of the present invention, there is provided a PCR amplification apparatus comprising: a mixer main chamber part in which a PCR buffer is accommodated and mixed with a gene extracted from a target organism; And a mixer auxiliary chamber portion connected to the mixer main chamber portion and adapted to receive the gene and the PCR buffer accommodated in the mixer main chamber portion, wherein the gene and the PCR buffer are connected to the mixer auxiliary chamber portion And the mixer main chamber portion is arranged to be mixed while reciprocating the mixer main chamber portion.

In an embodiment of the present invention, the mixer main chamber portion includes a mixer main chamber main body in which the gene and the PCR buffer are accommodated; And a mixer main chamber extension body extending from one side of the mixer main chamber main body and having a relatively higher stepped portion compared to the mixer main chamber main body.

In the embodiment of the present invention, the mixer main chamber portion includes a mixer injection hole formed on a bottom surface of the mixer main chamber main body and into which the gene is injected, and the PCR buffer is inserted into the mixer main chamber main body Wherein the mixer main chamber extension body is filled with the mixture from the mixer main chamber extension to the front end of the mixer injection hole.

In the embodiment of the present invention, the mixer main chamber portion may further include a mixer air filter provided on the upper side of the mixer injection hole, the mixer air filter being provided on the upper side of the main mixer main body, And the PCR buffer is mixed with the gene while discharging the bubbles injected together with the gene through the hole.

In the embodiment of the present invention, the mixer main chamber part further includes at least one vortex induction unit provided in the main mixer main body, and the vortex induction unit is configured to vortex when it collides with the gene and the PCR buffer And the like.

In the embodiment of the present invention, the vortex induction unit may be provided extending from both sides of the mixer main chamber body inwardly and spaced apart from each other.

The mixer transfer chamber unit may further include a mixer transfer chamber unit connected to the mixer auxiliary chamber unit. The mixer transfer chamber unit receives the mixed PCR solution in which the gene and the PCR buffer are mixed, Is discharged toward the dosing device.

In the embodiment of the present invention, the mixer pump unit may further include a mixer pump unit connected to the mixer transfer chamber unit, wherein the mixer pump unit mixes the gene and the PCR buffer to mixer main chamber unit And suction and discharge are performed.

In the embodiment of the present invention, the mixer pump unit sucks the mixed PCR solution accommodated in the mixer auxiliary chamber part and accommodates the mixed PCR solution in the mixer transfer chamber part, and mixes the PCR solution contained in the mixer transfer chamber part And air is injected to be discharged toward the injection device.

In an embodiment of the present invention, the mixer pump unit injects the PCR buffer into the mixer main chamber.

In the embodiment of the present invention, the mixer pressure regulating unit further includes a mixer pressure regulator connected to the mixer pump unit. When the air injection tube for injecting air into the mixer pump unit is connected, And the air between the air injection tube and the air injection tube is discharged to the outside.

In the embodiment of the present invention, the mixer pump unit and the mixer pressure adjusting unit may be provided at a position adjacent to the mixer pump unit and the mixer pressure adjusting unit such that when the air is sucked or discharged, Is formed.

In an embodiment of the present invention, the apparatus may further include a mixer valve unit provided between the mixer auxiliary chamber unit and the mixer transfer chamber unit.

In an embodiment of the present invention, the mixer valve portion includes a first mixer valve for controlling the flow between the mixer auxiliary chamber portion and the mixer transfer chamber portion; And a second mixer valve for controlling the flow between the mixer transfer chamber part and the dosing device.

According to an aspect of the present invention, there is provided a PCR buffer and a gene mixing method using a mixer device for gene discrimination chip, the method comprising the steps of: a) injecting the PCR buffer into the mixer main chamber; b) injecting the gene into the mixer main chamber portion into which the PCR buffer is injected; c) injecting and inhaling air so that the injected gene and the PCR buffer are mixed while reciprocating the mixer main chamber part and the mixer auxiliary chamber part. Gene mixing method.

In an embodiment of the present invention, after the step c), d) a step of sucking the mixed PCR solution in which the gene and the PCR buffer are mixed and transferring the mixed PCR solution to the mixer transfer chamber part; And e) injecting air into the mixer transfer chamber and transferring the mixed PCR solution to a dosing device.

In order to achieve the above object, the present invention provides a gene discrimination apparatus to which a mixer apparatus for a gene discrimination chip is applied.

In order to accomplish the above object, the present invention provides a food poisoning discriminator to which a mixer device for a gene discriminating chip is applied.

In order to achieve the above object, the present invention provides a fish species discriminator to which a mixer device for a gene discrimination chip is applied.

The effect of the present invention according to the above configuration is that the gene and the PCR buffer can be efficiently mixed while reciprocating the mixer main chamber part and the mixer auxiliary chamber part.

Also, the mixer air filter provided in the mixer main chamber can discharge the bubbles to be injected together with the gene injected through the mixer injection holes, blend the gene and the PCR buffer while the bubbles blow out to be discharged through the mixer air filter The mixing efficiency can be improved.

In addition, the vortex induction unit provided in the main chamber of the mixer can generate vortex when the gene and the PCR buffer reciprocate between the mixer main chamber portion and the mixer auxiliary chamber portion, thereby improving the mixing efficiency.

Further, the mixer pressure regulator is sealed after discharging the air between the mixer pump portion and the air injection tube to the outside when the air injection tube for injecting air into the mixer pump portion is connected. As a result, when the air injection tube is connected to the mixer pump unit, it is possible to prevent the air flowing between the mixer pump unit and the air injection tube from flowing into the mixer pump unit. That is, only a predetermined amount of air can always be injected.

In addition, the mixer support hole can prevent a problem that leakage occurs when the film substrate stacked in a plurality of layers spreads when air flows into the mixer pump portion and the mixer pressure control portion.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

FIG. 1 and FIG. 2 are schematic diagrams illustrating a configuration of a mixer device and a quantitative injection device for gene discrimination chip according to an embodiment of the present invention.
3 is an illustration of a mixer main chamber portion of a mixer device for gene discrimination chip according to an embodiment of the present invention.
FIG. 4 is a view illustrating an example of a vortex induction unit of a mixer apparatus for a gene discrimination chip according to an embodiment of the present invention.
FIG. 5 is a view illustrating an internal fluid flow of a mixer main chamber of a mixer device for gene discrimination chip according to an embodiment of the present invention.
6 is a flowchart of a PCR buffer and gene mixing method using a mixer apparatus for a chip for gene discrimination according to an embodiment of the present invention.
7 is a flowchart of a method for injecting a gene using a quantitative injection device for gene discrimination chip according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" (connected, connected, coupled) with another part, it is not only the case where it is "directly connected" "Is included. Also, when an element is referred to as " comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 and FIG. 2 are schematic diagrams illustrating a configuration of a mixer device and a quantitative injection device for gene discrimination chip according to an embodiment of the present invention. FIG. 3 is a view showing an example of a mixer main chamber part of a mixer device for gene discrimination chip according to an embodiment of the present invention, and FIG. 4 is a view showing an example of a vortex induction unit of a mixer device for gene discrimination chip according to an embodiment of the present invention. And FIG. 5 is an exemplary view showing an internal fluid flow of a mixer main chamber portion of a mixer device for a gene discrimination chip according to an embodiment of the present invention.

1 to 5, the mixer apparatus 200 for gene discrimination chip includes a mixer main chamber unit 210, a mixer auxiliary chamber unit 220, a mixer transfer chamber unit 230, a mixer pump unit 240, A mixer pressure adjusting unit 250, a mixer support hole 260 and a mixer valve unit 270. The gene and PCR buffer (polymerase chain reaction buffer) are connected to the mixer auxiliary chamber unit 220, (210).

The mixer main chamber portion 210 includes a mixer main chamber body 211, a mixer main chamber extension 212, a mixer injection hole 213, a mixer air filter 214 and a vortex induction unit 215, And the PCR buffer is accommodated and mixed with the gene extracted from the target bacteria.

The mixer main chamber main body 211 is provided so that the gene and the PCR buffer can be accommodated therein. Specifically, the mixer main chamber body 211 may be provided on a substrate formed by stacking a plurality of films, and a space in which the gene and the PCR buffer can be accommodated may be formed therein.

The mixer main chamber extension body 212 extends to one side of the mixer main chamber body 211 and a relatively higher step than the mixer main chamber main body 211 can be formed. The mixer main chamber extension 212 is connected to the mixer sub chamber 220 only when the mixer sub chamber 220 sucks the gene and the PCR buffer introduced into the mixer main chamber body 211. [ A step can be formed so as to be able to be transported. The mixer main chamber extension 212 may prevent the gene and the PCR buffer from moving to the mixer sub-chamber 220 when the mixer sub-chamber 220 does not apply a suction force.

The mixer injection hole 213 is formed on the bottom surface of the mixer main chamber body 211 and the extracted gene can be injected. 5 (a), the PCR buffer is injected from the mixer main chamber extension 212 before the gene is introduced into the mixer main chamber body 211, and is injected into the mixer injection hole 213 To the front end of the housing. That is, the mixer injection hole 213 provided in the mixer main chamber body 211 can prevent the PCR buffer pre-filled in the mixer main chamber body 211 from flowing into the mixer injection hole 213 and flowing back May be provided.

The mixer air filter 214 is provided on the upper side of the mixer main chamber body 211 and may be provided on the upper side of the mixer injection hole 213. The mixer air filter 214 mixes the gene and the PCR buffer while discharging the bubbles injected together with the gene through the mixer injection hole 213 to the outside. Specifically, the gene introduced through the mixer injection hole 213 includes bubbles. The mixer air filter 214 can discharge the bubbles introduced together with the gene to the outside. At this time, the bubble may burst and the gene and the PCR buffer may be mixed. That is, the mixer air filter 214 can improve mixing efficiency.

In addition, the mixer air filter 214 may increase the mixing efficiency as described above while discharging the bubbles generated while the gene and the PCR buffer reciprocate.

The vortex induction unit 215 may be provided in the mixer main chamber body 211 and the vortex induction unit 215 may be provided to vortex while colliding with the gene and the PCR buffer when they move . 4, the vortex induction unit 215 may be provided to extend inward from both sides of the mixer main chamber body 211, And is provided. The vortex induction unit 215 may be provided on one side of the mixer main chamber body 211 and on the other side of the mixer main chamber body 211 so as to be offset from each other.

4 (a), the vortex induction unit 215 may be provided to extend vertically inward from both sides of the mixer main chamber body 211, and as shown in FIG. 4 (b) and may be formed to extend diagonally inward from both side surfaces of the mixer main chamber body 211 as shown in Figs.

4 (d), the vortex induction unit 215 may be formed to extend inwardly from both sides of the mixer main chamber body 211 in a curved shape.

However, the vortex induction unit 215 is not limited to the illustrated form, and may be included in one embodiment as long as the shape and position of vortex can be actively generated when the gene and the PCR buffer move.

The vortex induction unit 215 thus provided can improve the mixing efficiency of the gene and the PCR buffer.

Since the mixer main chamber body 211 provided as described above is provided on the substrate formed by lamination of the thin films, the mixing of the gene and the PCR buffer in the same space is not performed quickly. However, the mixer main chamber part 210 according to the present invention is configured to rapidly mix the gene and the PCR buffer, so that the mixed PCR solution can be rapidly formed.

The mixer auxiliary chamber unit 220 is connected to the mixer main chamber unit 210 to receive the gene and the PCR buffer accommodated in the mixer main chamber unit 210.

The mixer transfer chamber part 230 is connected to the mixer auxiliary chamber part 220 and the mixer transfer chamber part 230 can be provided to receive a mixed PCR solution in which the gene and the PCR buffer are mixed. The mixer transfer chamber 230 may be connected to the dosing device 300 so that the mixed PCR solution can be discharged toward the dosing device 300.

The mixer pump unit 240 is connected to the mixer transfer chamber unit 230 and the mixer pump unit 240 mixes the gene and the PCR buffer with the mixer auxiliary chamber unit 220 and the mixer main chamber unit 210 to reciprocate and mix air. 5 (b), the mixer pump unit 240 sucks the mixed PCR solution contained in the mixer main chamber 210 and moves the mixed PCR solution to the mixer auxiliary chamber unit 220, 5C, air may be injected into the mixed PCR solution accommodated in the mixer sub-chamber 220 and transferred to the mixer main chamber 210. As shown in FIG. In this way, the mixer pump unit 240 can mix the gene and the PCR buffer between the mixer sub-chamber 220 and the mixer main chamber 210 and mix them.

In addition, the mixer pump unit 240 may suck the mixed PCR solution contained in the mixer auxiliary chamber unit 220 and store the mixed PCR solution in the mixer transfer chamber unit 230. The mixer pump unit 240 may be provided to inject air to discharge the mixed PCR solution contained in the mixer transfer chamber unit 230 toward the dosing device 300.

In addition, the mixer pump unit 240 may inject the PCR buffer into the mixer main chamber 210. Specifically, the mixer pump unit 240 may inject a preset amount of PCR buffer into the mixer main chamber 210 before the gene is injected.

The mixer pressure regulator 250 is connected to the mixer pump 240 and the mixer pressure regulator 250 includes an air injection tube for injecting air into the mixer pump 240, The air between the mixer pump unit 240 and the air injection tube is discharged to the outside, and then the air is sealed. When the air injection tube is connected to the mixer pump unit 240, the air supplied from the mixer pump unit 240 to the air injection tube is supplied to the mixer pump unit 240, Can be prevented. That is, only a predetermined amount of air can always be injected.

The mixer pressure regulator 250 may be opened when the mixer pump 240 discharges the mixed PCR solution to the quantitative injection device 300 to adjust the internal pressure. The mixer pressure regulator 250 may be separated from the mixer pump 240 in a state in which the internal pressure of the mixer pressure regulator 250 is adjusted, thereby improving safety.

The mixer support hole 260 may be formed at a position adjacent to the mixer pump 240 and the mixer pressure regulator 250. The mixer support hole 260 may prevent a substrate on which a plurality of films are stacked from being deformed when air flows into the mixer pump unit 240 and the mixer pressure control unit 250. In addition, the mixer support hole 260 prevents the substrate from being deformed, thereby preventing leakage of water.

The mixer valve unit 270 is provided between the mixer auxiliary chamber unit 220 and the mixer transfer chamber unit 230 and includes a first mixer valve 271 and a second mixer valve 272.

The first mixer valve 271 may control the flow between the mixer auxiliary chamber part 220 and the mixer transfer chamber part 230.

The second mixer valve 272 may control the flow between the mixer transfer chamber part 230 and the dosing device 300.

The first mixer valve 271 may be configured such that the mixer pump unit 240 sucks the mixed PCR solution contained in the mixer auxiliary chamber unit 220 and transfers the mixed PCR solution to the mixer transfer chamber unit 230 It may be in an open state. At this time, the second mixer valve 272 is closed.

The second mixer valve 272 may be opened when the mixer pump unit 240 transfers the mixed PCR solution contained in the mixer transfer chamber unit 230 to the dosing device 300. At this time, the first mixer valve 271 is closed.

The mixer device 200 for gene discrimination prepared as described above can be applied to a gene discriminator, a food poisoning discriminator, a fish species discriminator, and the like.

6 is a flowchart of a PCR buffer and gene mixing method using a mixer apparatus for a chip for gene discrimination according to an embodiment of the present invention.

Hereinafter, a PCR buffer and gene mixing method using a mixer apparatus for a gene discriminating chip will be described with reference to FIG.

First, a PCR buffer and a gene mixing method using a mixer device for gene discrimination chip can perform a step S210 of injecting a PCR buffer into a mixer main chamber. In this step, the PCR buffer is injected from the mixer main chamber extension 212 by the mixer pump unit 240 before the gene is introduced into the mixer main chamber body 211, To the front end of the housing.

Next, a step S220 of injecting a gene into the mixer main chamber portion into which the PCR buffer is injected may be performed. At this stage, the gene injected through the mixer injection hole 213 may be mixed with the PCR buffer while bubbles are discharged by the mixer air filter 214 formed in the upper part of the mixer injection hole 213 .

Next, a step S230 of injecting and sucking air such that the injected gene and the PCR buffer are mixed while reciprocating the mixer main chamber part and the mixer auxiliary chamber part can be performed. 5 (b), the mixer pump unit 240 sucks the mixed PCR solution contained in the mixer main chamber 210 and moves the mixed PCR solution to the mixer auxiliary chamber unit 220, 5C, air may be injected into the mixed PCR solution accommodated in the mixer sub-chamber 220 and transferred to the mixer main chamber 210. As shown in FIG. As described above, the mixer pump unit 240 may mix the gene and the PCR buffer by reciprocating the mixer auxiliary chamber unit 220 and the mixer main chamber unit 210 a plurality of times.

In addition, when the gene and the PCR buffer are mixed while reciprocating between the mixer auxiliary chamber part 220 and the mixer main chamber part 210, they may collide with the vortex induction unit 215 to generate a vortex. The resulting vortex can improve the mixing efficiency of the gene and the PCR buffer.

Next, the mixed PCR solution in which the gene and the PCR buffer are mixed may be sucked and transferred to the mixer transfer chamber part (S240). In this stage, the mixer transfer chamber unit 230 can receive and receive the mixed PCR solution contained in the mixer auxiliary chamber unit 220 by the suction force of the mixer pump unit 240.

Next, air may be injected into the mixer transfer chamber to transfer the mixed PCR solution to the dosing device (S250). At this stage, the mixer pump unit 240 may inject air to discharge the mixed PCR solution contained in the mixer transfer chamber unit 230 toward the dosing device 300.

The PCR buffer and gene mixing method using the mixer device for gene discrimination chip prepared as described above can improve the reliability of the experimental result and improve the mixing efficiency of the gene and the PCR buffer,

1 and 2, the quantitative injection device 300 for a gene discriminating chip includes a quantification chamber 310, a quantitative stopper 320, a residual amount discharging unit 330, a quantitative pump 340, A support hole 350, a metering valve portion 360, and a metering air filter portion 370.

The quantification chamber 310 has a plurality of quantification chambers for receiving a mixed PCR solution formed by mixing a gene and a PCR buffer. Specifically, the metering chamber 310 includes a first metering chamber 311, a second metering chamber 312, a third metering chamber 313, a fourth metering chamber 314, and a fifth metering chamber 315, . The first to third quantification chambers 311 to 315 may be provided to have a size capable of accommodating a predetermined amount of the mixed PCR solution.

The first quantification chamber 311 is provided on the upstream side of the flow of the mixed PCR solution and is connected to the quantification chamber 312, the third quantification chamber 313, the fourth quantification chamber 314 And the fifth metering chamber 315 may be sequentially arranged. The number of the metering chamber units 310 is not limited to one embodiment but may be plural.

The metering stopper 320 is provided between adjacent pair of metering chambers, and has a plurality of metering stoppers having a relatively higher stepped portion compared to the metering chambers. A first metering stopper 321 is provided between the first metering chamber 311 and the second metering chamber 312 and the second metering chamber 312 and the third metering chamber 313 are provided between the first metering chamber 311 and the second metering chamber 312, A second metering stopper 322 is provided. A third metering stopper 323 is provided between the third metering chamber 313 and the fourth metering chamber 314 and between the fourth metering chamber 314 and the fifth metering chamber 315 A fourth metering stopper 324 is provided.

The quantitative stopper unit 320 may be provided with a stepped portion for allowing the mixed PCR solution to pass therethrough in a state where the mixed PCR solution is filled in the quantitative chamber located upstream. The first quantitative stopper 321 to the fourth quantitative stopper 324 of the quantitative stopper 320 may be formed so as to form steps having the same height. In the path of the mixed PCR solution, .

For example, the introduced mixed PCR solution is first directed to the first quantitative stopper 321. However, since the first quantitative stopper 321 has a stepped height, the mixed PCR solution can not pass over the first quantitative stopper 321 and is naturally transferred to the first quantitative chamber 311, And is filled in the first metering chamber 311. When the first quantitative chamber 311 is filled with the mixed PCR solution, the water level of the mixed PCR solution rises by the step height of the first quantitative stopper 321, and the first quantitative stopper 321 It passes over. The mixed PCR solution having passed through the first quantitative stopper 321 is directed to the second quantitative stopper 322. However, since the second quantitative stopper 322 also has a stepped height, the mixed PCR solution moves toward the second quantitative chamber 312 without passing over the second quantitative stopper 322, The chamber 312 is filled. When the second quantitative chamber 312 is filled with the mixed PCR solution, the water level of the mixed PCR solution rises by the step height of the second quantitative stopper 322, and the second quantitative stopper 322 It passes over. In this manner, the third PCR chamber 313 and the fourth PCR chamber 314 can be filled with the mixed PCR solution.
In addition, since the quantitative stopper 320 is not provided in a stepped shape and the wall surface of the channel channel is made hydrophobic, the mixed PCR solution can be injected in a predetermined amount into each of the metering chambers in accordance with the difference in fluid resistance in the channel channel. .

The remaining amount discharging unit 330 may be provided to discharge the mixed PCR solution remaining after filling the metering chamber unit 310. [

The metering stopper 320 further includes a discharge stopper 325 provided between the metering chamber 310 and the residual amount discharging unit 330. Specifically, the discharge stopper 325 is provided between the fifth metering chamber 315 and the remaining amount discharging portion 330, and is relatively higher than the metering stoppers 321, 322, 323, Can be formed. In the same manner as described above, the discharge stopper 325 may be discharged to the remaining amount discharging unit 330 through the mixed PCR solution while the mixed PCR solution is completely filled in the metering chamber 310 So that a stepped portion is formed.

The quantitative chamber 310, the quantitative stopper 320, and the residual amount discharger 330 provided as described above may be filled with a predetermined mixed PCR solution in each of the quantitative chambers without any separate control. That is, according to the present invention, the mixed PCR solution injected into the amplifying device 400 can be kept constant, thereby improving the accuracy of the experiment, and the mixed PCR solution can be injected quickly, which is economical.

The metering pump unit 340 is provided on the upstream side of the metering chamber unit 310 and may transfer the mixed PCR solution contained in the metering chamber unit 310 to the amplifying unit 400. More specifically, the metering pump unit 340 is provided between the metering chamber unit 310 and the metering stopper unit 320. When the metering chamber unit 310 is filled with the mixed PCR solution, So that the mixed PCR solution contained in the quantification chamber 310 can be transferred to the amplification unit 400.

The quantitative support hole 350 may be formed at a position adjacent to the quantitative pump 340. When the air is injected into the metering pump unit 340, the quantitative support hole 350 is prevented from being deformed due to the widening of the substrate. In addition, the quantitative support hole 350 prevents the substrate from being deformed, thereby preventing leakage of water.

The metering valve unit 360 is provided on the upstream side of the metering pump unit 340. The metering valve unit 360 may be disposed between the metering pump unit 340 and the metering stopper unit 320. The metering valve unit 360 may be disposed between the metering pump unit 340 and the metering stopper unit 320, And may be in a closed state when transferring the mixed PCR solution contained in the metering chamber portion 310 to the amplification device 400. [

The quantitative air filter unit 370 is provided on the downstream side of the quantification chamber unit 310 and the quantitative air filter unit 370 is disposed on the downstream side of the quantitative air filter unit 370, Can be removed. Specifically, when the amplification device 400 amplifies the gene contained in the mixed PCR solution, if the bubble is included, an error may occur in the experimental result. Therefore, the quantitative air filter unit 370 can remove the bubbles contained in the mixed PCR solution transferred toward the amplification unit 400, thereby preventing the above-described problems from occurring.

In addition, the quantitative air filter unit 370 may be configured to discharge all of the air injected from the metering pump unit 340 to stop the transfer of the mixed PCR solution when the mixed PCR solution has passed through (370) . The metering pump unit 340 injects air and the mixed PCR solution contained in the metering chamber unit 310 is transferred to the amplifying unit 400. The metering pump unit 340 injects the mixed PCR solution, Air can be exhausted through the quantitative air filter unit 370. The quantitative air filter unit 370 may prevent the mixed PCR solution contained in the amplification unit 400 from being pressurized even when the metering pump unit 340 continuously injects air. Accordingly, the quantitative air filter unit 370 is not required to precisely control the injection time of the air for feeding the mixed PCR solution by the quantitative pump unit 340, which is convenient.

The quantitative injection device 300 for gene identification provided as described above can be applied to a gene discrimination device, a food poisoning discriminator, a fish species discriminator, and the like.

7 is a flowchart of a method for injecting a gene using a quantitative injection device for gene discrimination chip according to an embodiment of the present invention.

Hereinafter, a gene quantitative injection method using a quantitative injection device for gene discrimination chip will be described with reference to FIG.

In the method of injecting a gene using a quantitative injection device for gene discrimination chip, a step S310 of sequentially filling the mixed PCR solution from a quantification chamber located upstream can be performed. This step may be performed automatically by the quantitative stopper having a stepped portion through which the mixed PCR solution is passed after the mixed PCR solution is filled in the quantification chamber located upstream.

For example, the introduced mixed PCR solution is first directed to the first quantitative stopper 321. However, since the first quantitative stopper 321 has a stepped height, the mixed PCR solution can not pass over the first quantitative stopper 321 and is naturally transferred to the first quantitative chamber 311, And is filled in the first metering chamber 311. When the first quantitative chamber 311 is filled with the mixed PCR solution, the water level of the mixed PCR solution rises by the step height of the first quantitative stopper 321, and the first quantitative stopper 321 It passes over. The mixed PCR solution having passed through the first quantitative stopper 321 is directed to the second quantitative stopper 322. However, since the second quantitative stopper 322 also has a stepped height, the mixed PCR solution moves toward the second quantitative chamber 312 without passing over the second quantitative stopper 322, The chamber 312 is filled. When the second quantitative chamber 312 is filled with the mixed PCR solution, the water level of the mixed PCR solution rises by the step height of the second quantitative stopper 322, and the second quantitative stopper 322 It passes over. In this manner, the third PCR chamber 313 and the fourth PCR chamber 314 can be filled with the mixed PCR solution.

Next, a step S320 of filling the remaining portion of the metering chamber and discharging the remaining mixed PCR solution to the remaining amount discharging portion may be performed. At this stage, the discharge stopper 325 (see FIG. 3), which has a stepped portion to allow the excess mixed PCR solution to pass therethrough and be discharged to the remaining amount discharge portion 330 in a state in which the mixed PCR solution is filled in the dosing chamber portion 310 ). In this case, as shown in Fig.

Next, a step S330 of closing the metering valve unit may be performed.

Next, air is injected into the metering pump unit, and the mixed PCR solution contained in the metering chamber unit is injected into the amplifying unit (S340). At this stage, when the mixed PCR solution is completely filled in the quantification chamber part 310, the quantitative pump part 340 injects air to amplify the mixed PCR solution contained in the quantification chamber part 310, To the device (400). At this time, the quantitative air filter unit 370 can remove bubbles contained in the mixed PCR solution conveyed toward the amplification unit 400. When the mixed PCR solution passes through the quantitative air filter unit 370, The mixed PCR solution may be stopped to discharge the air injected from the mixing chamber 340 to stop the transfer of the mixed PCR solution.

The method of injecting a gene using the quantitative injection device for gene discrimination chip as described above can always be injected into the amplifying device 400 by a predetermined amount so as to increase the reliability of the experimental results and automatically So that a convenient and rapid experiment can be performed.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

200: Mixer device for gene discrimination chip 210: Mixer main chamber part
211: Mixer main chamber body 212: Mixer main chamber extension
213: Mixer injection hole 214: Mixer air filter
215: vortex induction unit 220: mixer auxiliary chamber part
230: mixer transfer chamber part 240: mixer pump part
250: Mixer pressure regulator 260: Mixer support hole
270: mixer valve unit 271: first mixer valve
272: Second mixer valve 300: Quantitative injection device for gene discrimination chip
310: Quantification chamber section 311: First quantification chamber
312: Second Quantification Chamber 313: Third Quantification Chamber
314: fourth determination chamber 315: fifth determination chamber
320: Quantitative stopper part 321: First quantitative stopper
322: second quantitative stopper 323: third quantitative stopper
324: Fourth quantitative stopper 325: Discharge stopper
330: remaining amount discharging part 340:
350: Quantitative support hole 360: Quantitative valve section
361: first metering valve 362: second metering valve
363: third metering valve 364: fourth metering valve
365: fifth metering valve 370: metering air filter section
371: first quantitative air filter 372: second quantitative air filter
373: Third quantitative air filter 374: Fourth quantitative air filter
375: fifth quantitative air filter 400: amplifying device

Claims (19)

A mixer main chamber part in which PCR buffer is accommodated and mixed with genes extracted from the detection target bacteria; And
And a mixer auxiliary chamber portion connected to the mixer main chamber portion and adapted to receive the gene and the PCR buffer accommodated in the mixer main chamber portion,
The gene and the PCR buffer are arranged to be mixed while reciprocating between the mixer auxiliary chamber part and the mixer main chamber part,
The mixer main chamber portion includes:
A mixer main chamber body in which the gene and the PCR buffer are accommodated; And
And at least one vortex induction unit provided in the mixer main chamber body,
Wherein the vortex induction unit is configured to generate a vortex while colliding with the gene and the PCR buffer when moving. The method according to claim 1,
The mixer main chamber portion includes:
Gene chips determine mixer apparatus is provided extending to one side of the mixer main chamber body, it characterized in that it further comprises a mixer main chamber yeonjangche relatively high compared to the level difference formed in the mixer main body chamber. 3. The method of claim 2,
The mixer main chamber portion includes:
And a mixer injection hole formed on a bottom surface of the main body of the mixer for injecting the gene,
Wherein the PCR buffer is filled from the mixer main chamber extension to the front end of the mixer injection hole before the gene is introduced into the mixer main chamber main body. The method of claim 3,
The mixer main chamber portion includes:
Further comprising a mixer air filter provided on the upper side of the mixer main chamber body and provided on an upper portion of the mixer injection hole,
Wherein the mixer air filter mixes the gene with the PCR buffer while discharging the bubbles injected together with the gene through the mixer injection hole to the outside. delete 3. The method of claim 2,
The vortex induction unit includes:
Wherein the mixer main chamber body is provided so as to extend inward from both sides of the mixer main chamber body, and are spaced apart from each other. The method according to claim 1,
And a mixer transfer chamber part connected to the mixer auxiliary chamber part,
Wherein the mixer transfer chamber part receives the mixed PCR solution in which the gene and the PCR buffer are mixed and discharges the mixed PCR solution to the quantitative injection device. 8. The method of claim 7,
And a mixer pump unit connected to the mixer transfer chamber unit,
Wherein the mixer pump unit sucks and discharges air such that the gene and the PCR buffer are mixed while reciprocating between the mixer auxiliary chamber unit and the mixer main chamber unit. 9. The method of claim 8,
The mixer pump unit includes:
The mixing PCR solution accommodated in the mixer auxiliary chamber portion is sucked and accommodated in the mixer transfer chamber portion and air is injected to discharge the mixed PCR solution accommodated in the mixer transfer chamber portion toward the dosing device Wherein the mixer comprises: 9. The method of claim 8,
The mixer pump unit includes:
And the PCR buffer is injected into the main chamber of the mixer. 9. The method of claim 8,
A mixer pressure adjusting unit connected to the mixer pump unit,
Wherein the mixer pressure regulating unit is configured such that when the air injection tube for injecting air into the mixer pump unit is connected, the air between the mixer pump unit and the air injection tube is discharged to the outside, Mixer device for chips. 12. The method of claim 11,
Wherein a mixer support hole is formed at a position adjacent to the mixer pump unit and the mixer pressure adjuster to prevent the substrate from being deformed when sucking or discharging air through the mixer pump unit and the mixer pressure adjuster. Mixer device for discrimination chip. 9. The method of claim 8,
Further comprising a mixer valve unit provided between the mixer auxiliary chamber unit and the mixer transfer chamber unit. 14. The method of claim 13,
The mixer-
A first mixer valve for controlling the flow between the mixer auxiliary chamber portion and the mixer transfer chamber portion; And
And a second mixer valve for controlling the flow between the mixer transfer chamber and the dosing device. A PCR buffer and gene mixing method using the mixer device for chip discrimination according to claim 1,
a) injecting the PCR buffer into the mixer main chamber;
b) injecting the gene into the mixer main chamber portion into which the PCR buffer is injected; And
c) injecting and inhaling air so that the injected gene and the PCR buffer are mixed while reciprocating the mixer main chamber part and the mixer auxiliary chamber part. Gene mixing method. 16. The method of claim 15,
After step c)
d) transferring the mixed PCR solution containing the gene and the PCR buffer into a mixer transfer chamber part; And
and e) injecting air into the mixer transfer chamber unit to transfer the mixed PCR solution to a dosing device. The method of mixing a PCR buffer and a gene using a mixer device for a gene identification chip. A gene discrimination apparatus to which a mixer apparatus for chip discrimination according to claim 1 is applied. A food poisoning discriminator to which a mixer device for chip discrimination according to claim 1 is applied. A fish species discriminator to which a mixer device for chip discrimination according to claim 1 is applied.

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