KR101894100B1 - Bio sensor - Google Patents

Abstract

The present invention relates to a biosensor. A biosensor according to an embodiment of the present invention includes a lower plate including a detection unit, an upper plate coupled to the lower plate and including a first upper plate and a second upper plate positioned side by side, Wherein the first upper plate includes a sample injection portion formed at a position corresponding to the filter and a first projection projecting toward the second upper plate at a side adjacent to the second upper plate, The upper plate includes a second projection projecting toward the first upper plate at a side adjacent to the first upper plate, and the first upper plate and the second upper plate are coupled by the joining of the first projection and the second projection. Thereby, it is possible to prevent leakage of the liquid sample and to rapidly separate the analyte from the sample.

Description

Biosensor {Bio sensor}

The present invention relates to a biosensor, and more particularly, to a biosensor capable of preventing the leakage of a liquid sample and quickly separating the analyte from the sample.

As the life expectancy of modern people increases, the kinds of diseases that accompany them have also become diverse, and various diagnosis devices and diagnosis systems for the prevention and diagnosis of diseases have been developed.

Among them, the in vitro diagnostic apparatuses can detect the substance to be analyzed using the body fluids of the human body such as blood and urine as a sample, and can quantitatively analyze the presence or absence of the disease quickly, so that promptness, efficiency, And so on.

On the other hand, the biosensor, which can be used variously from the diagnosis of pregnancy to the inspection of various diseases such as cancer and multiple sclerosis, uses minute proteins and DNA such as antibodies, so that the accuracy of the biosensor becomes an important task have.

However, the conventional biosensor has a structure in which a filter is inserted between a flat upper plate and a lower plate, thereby generating a space that is not in contact with the upper plate and the lower plate by the thickness of the filter, When a physical joining process is performed in a state where there is a gap between the upper plate and the lower plate, distortion occurs in the upper plate and the lower plate. Thereby, a relatively strong pressure is applied to a part of the filter, and leakage of the specimen in the biosensor may occur before the filter separates the analyte from the specimen by the increased capillary force. Therefore, the accuracy of the biosensor may be lowered.

On the other hand, JP-A-2009-0049414 discloses a plasma separation filter element for minimizing the outflow of blood cells, while the plasma separation filter element disclosed in JP-A-2009-0049414 has a separate The O-ring-shaped upper elastic plate and the lower elastic plate are laminated with the filter portion and then buried in the upper plate or the lower plate.

Published Japanese Patent Application No. 2009-0049414

SUMMARY OF THE INVENTION An object of the present invention is to provide a biosensor capable of preventing leakage of a liquid sample and rapidly separating the analyte from the sample.

According to an aspect of the present invention, there is provided a biosensor including a lower plate including a detection unit, an upper plate coupled to the lower plate and including a first upper plate and a second upper plate, And a filter disposed between the lower plate and the upper plate, wherein the first upper plate includes a sample insertion portion formed at a position corresponding to the filter, and a first protrusion protruding toward the second upper plate at a surface adjacent to the second upper plate, And the second upper plate includes a second projection projecting toward the first upper plate at a side adjacent to the first upper plate, wherein the first upper plate and the second upper plate have a first protrusion and a second protrusion They are joined by bonding.

Further, the lower surface of the first projecting portion and the upper surface of the second projecting portion are joined.

Further, the second projecting portion is formed with an inclined surface.

Further, the filter has one end extended on the inclined surface and bent upward, and one end of the filter is positioned between the first projection and the second projection.

Further, the first upper plate includes a pressing portion protruding toward the filter at the outer periphery of the sample introducing portion.

A vent hole is formed in the first projection.

Further, the first upper plate is subjected to a hydrophobic surface treatment, and the second upper plate is subjected to a hydrophilic surface treatment.

Further, the lower plate is formed with fillers contacting the bottom surface of the filter.

Further, the fillers include a first filler and a second filler larger than the first filler, and the second filler is formed to correspond to the position of the pressing portion.

According to another aspect of the present invention, there is provided a biosensor including an upper plate, a lower plate coupled with the upper plate, and a filter positioned between the upper plate and the lower plate, A first upper plate and a second upper plate joined together positioned side by side, the first upper plate including a sample injection part formed at a position corresponding to the filter, and a second injection part projecting toward the second upper plate at a surface adjacent to the second upper plate And the second upper plate includes a second projection projecting toward the first upper plate at a surface adjacent to the first upper plate, the lower surface of the first projection and the upper surface of the second projection are joined, One end of the filter extends toward the second top plate and is positioned between the first projection and the second projection.

Further, the second projecting portion is formed on the inclined surface, and one end of the filter is positioned on the inclined surface.

Further, the first upper plate includes a pressing portion protruding toward the filter at the outer periphery of the sample introducing portion.

Further, the lower plate includes fillers contacting the bottom surface of the filter, the fillers include a first filler and a second filler larger than the first filler, and the second filler is formed to correspond to the position of the pressurizing portion.

Further, the first upper plate is subjected to a hydrophobic surface treatment, and the second upper plate and the lower plate are subjected to hydrophilic surface treatment.

According to one embodiment of the present invention, it is possible to prevent leakage of a liquid sample and to quickly separate the analyte from the sample.

1 is a perspective view illustrating a biosensor according to an embodiment of the present invention,
FIG. 2 is a perspective view showing a first upper plate of the biosensor of FIG. 1,
3 is a cross-sectional view of the BB 'section of FIG. 2,
FIG. 4 is a perspective view showing a second upper plate of the biosensor of FIG. 1,
FIG. 5 is a perspective view illustrating a lower plate of the biosensor of FIG. 1,
6 is a cross-sectional view taken along the line AA 'in FIG.

Hereinafter, the present invention will be described in detail with reference to the drawings.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention described below will be clarified with reference to the embodiments described below in detail with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Is provided to fully convey the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims. It is to be noted, however, that the terminology used herein is for the purpose of illustrating embodiments and is not intended to be limiting of the present invention. In the present specification, the singular form includes plural forms unless otherwise specified in the specification. It is noted that the terms "comprises" and / or "comprising" used in the specification are intended to be inclusive in a manner similar to the components, steps, operations, and / Or additions. Also, the terms first, second, etc. may be used to describe various elements, but the elements should not be limited by terms. Terms are used only for the purpose of distinguishing one component from another.

FIG. 1 is a perspective view illustrating a biosensor according to an embodiment of the present invention, FIG. 2 is a perspective view illustrating a first upper plate of the biosensor of FIG. 1, FIG. 3 is a cross- FIG. 4 is a perspective view of the second upper plate of the biosensor of FIG. 1, FIG. 5 is a perspective view of the lower plate of the biosensor of FIG. 1, and FIG. 6 is a cross- .

In the following, the biosensor may be any biological measurement device as long as one aspect can be embodied. For example, the biosensor may be, but is not limited to, a microarray chip, a lateral flow assay kit, and the like.

1 to 6, a biosensor 10 according to an embodiment of the present invention includes a lower plate 200, an upper plate 100 coupled with the lower plate 200, and a lower plate 200, And a filter 130 positioned between the top plate 100 and the top plate 100.

The upper plate 100 and the lower plate 200 are coupled to each other to form a channel 210 which is a moving passage of a liquid sample. Here, a specimen means a solution to be detected, which means a substance suspected of containing an analyte. For example, the sample may be any biological source such as a physiological fluid, including blood, saliva, cerebrospinal fluid, sweat, urine, milk, ascites, mucus, nasal fluid, hemoptysis, joint blood, peritoneal fluid, For example, a person, an animal, etc.).

In addition, a sample can be obtained directly from a biological source, or can be used after pretreatment to modify the characteristics of the sample. The pretreatment may include filtration, precipitation, dilution, mixing, concentration, inactivation of interference components, lysis, addition of reagents, and the like. As an example, measures such as separating plasma from blood may be performed.

The upper plate 100 and the lower plate 200 may be formed of a non-reactive material that does not react with a sample such as polycarbonate (PC), polystyrene (PS), polypropylene (PP), polymethyl methacrylate PMMA), or the like.

The upper plate 100 and the lower plate 200 may be bonded together by a hot bonding method, an epoxy bonding method, a chemical bonding method, an ultrasonic bonding method, a plasma bonding method, a solvent bonding method, The plates 200 may be surrounded by a label (not shown) while being coupled to each other.

Although the upper plate 100 and the lower plate 200 have the same size, the upper plate 100 and the lower plate 200 are not limited thereto. 210 may be formed on the lower plate 200. The lower plate 200 may have concave steps formed on the upper surface thereof and an upper plate 100 having a smaller size than the lower plate 200 may be seated on the stepped portions. Alternatively, the upper plate 100 may be formed larger than the lower plate 200.

The upper plate 100 may be formed of a light transmitting material. When the upper plate 100 is formed of a light transmitting material, it is possible to confirm both the flow of the specimen and the results of the detection unit 240 on the channel 210, You can omit the sight window.

The top plate 100 may be configured to include a first top plate 110 and a second top plate 120 positioned side by side. That is, the upper plate 100 is divided into a first upper plate 110 located on the filter 130 and a second upper plate 120 covering the upper surface of the remaining lower plate 200, The first upper plate 110 and the lower plate 200 are prevented from being twisted due to the thickness of the filter 130 when the upper plate 110 and the lower plate 200 are joined together, The phenomenon can be prevented. On the other hand, depending on the type of the specimen, for example, if the specimen is blood, the first upper plate 110 may be subjected to hydrophobic surface treatment and the second upper plate 120 may be subjected to hydrophilic surface treatment for rapid movement of the specimen . The hydrophilic surface treatment may be, for example, an EDTA coating treatment or a heparin coating treatment.

The first upper plate 110 is located on the filter 130 and the first upper plate 110 includes a sample inlet 114, a first upper plate 110, A vent hole 116, and a pressurizing portion 118. The first protrusion 112 may be formed to extend from one side of the first protrusion 112, the vent hole 116,

The sample injecting unit 114 may be an opening formed by removing a part of the first upper plate 110 to inject the specimen into the biosensor 10. The sample injecting section 114 is formed so as to correspond to the position of the filter 130.

Meanwhile, although not shown in the drawing, a cover (not shown) having a hole at the center may be coupled to the sample injecting unit 114. A cover (not shown) prevents the filter 130 from being exposed to the outside, thereby preventing the filter 130 from being contaminated. When a specimen is introduced through the specimen injecting unit 114, for example, It is possible to prevent the filter 130 from being damaged by the direct contact of the filter 130.

Since the hole is formed in the center of the cover (not shown), the inserted specimen is always absorbed by the filter 130 at a predetermined position, thereby reducing the error of the inspection result and increasing the accuracy. Can be improved. To this end, the cover (not shown) may have a concave shape at the central portion where the holes are formed.

1, one end of the first upper plate 110 adjacent to the second upper plate 120 may protrude toward the second upper plate 120. The first protrusion 112 has a second protrusion 112 protruding from the second upper plate 120. The first protrusion 112 protrudes from the second upper plate 120, 122, the first upper plate 110 and the second upper plate 120 are engaged.

The vent hole 116 may be formed in the first projection 112. The extended portion 132 of the filter 130 is positioned below the vent hole 116 as described later. Accordingly, the filter 130 is exposed to the atmospheric pressure by the vent hole 116, so that the sample in the channel 210 can smoothly flow.

The pressing portion 118 may protrude from the bottom surface of the first upper plate 110 toward the filter 130. The pressing portion 118 is formed on the outer periphery of the sample input portion 114 and the shape of the pressing portion 118 can correspond to the shape of the filter 130. [

Such a pressing portion 118 fixes the filter 130 and the capillary force is increased by pressing the filter 130 so that the sample can quickly come out of the filter 130.

3, a groove 119 may be formed on the lower surface of the first upper plate 110 to receive the filter 130, and the pressing portion 118 may be formed in the groove 119 . When the filter 130 is seated in the groove 119 formed in the lower surface of the first upper plate 110, when the first upper plate 110 and the lower plate 200 are coupled, 1 partial distortion of the upper plate 110 and the lower plate 200 can be more effectively prevented.

The second upper plate 120 is coupled with the lower plate 200 to form a channel 210. Further, for example, when the sample is blood, the second upper plate 120 may be subjected to a hydrophilic surface treatment for rapid movement of the sample.

The second upper plate 120 may include a second protrusion 122 protruding toward the first upper plate 110 at a side adjacent to the first upper plate 110.

As shown in FIG. 1, the second protrusion 122 may be formed by protruding the lower side of one surface of the second upper plate 120, which is adjacent to the first upper plate 110, toward the first upper plate 110 have. Accordingly, the second upper plate 120 may have a cross-sectional shape in a section where the second projections 122 are formed, and the upper surface of the second projections 122 may extend from the first upper plate 110 And can be joined to the lower surface of the protruding first projection 112.

On the other hand, as shown in FIG. 4, the inclined surface 124 may be formed on the second projection 122. The sloped surface 124 may be formed in a part of the area of the second projection 122, particularly in the central part.

The filter 130 extends at one end toward the second upper plate 120 and the extended portion 132 of the filter 130 contacts the upper surface of the second upper plate 120, Lt; / RTI > Accordingly, the extended portion 132 of the filter 130 is bent upward on the inclined surface 124, whereby the specimen which has flowed along the upper surface of the filter 130 and the lower surface of the first upper plate 110 It is possible to effectively prevent the phenomenon of leaking from one end of the filter 130. [

The extended portion 132 of the filter 130 located on the sloped surface 124 is located between the first projection 112 and the second projection 122 to form the first projection 112 and the second projection 122. [ (Not shown). Thereby, the filter 130 is more effectively fixed.

The lower plate 200 shown in FIG. 5 may be formed of the same material as the upper plate 100, and may be coupled with the upper plate 100 to form a channel 210, which is a path of movement of the sample. The lower plate 200 includes a seating part 220 where the filter 130 is located, a detection part 240 formed on the channel 210, a storage chamber 240 for storing the unreacted sample residue in the detection part 240, (250), and the like.

The seating part 220, the channel 210, and the storage chamber 250 may be formed to have a lower height than the surface of the lower plate 200, but the present invention is not limited thereto. For example, the seating part 220, the channel 210 and the storage chamber 250 have the same height as the lower plate 200, and a wall part (not shown) for partitioning them is formed in the lower plate 200 .

The seating part 220 is a region in which the filter 130 corresponding to the groove 119 formed in the lower surface of the first upper plate 110 is located and the seating part 220 is a region in which the sample 130, The remaining three sides have a clogged structure so that they can move only in one direction.

Fillers 222 and 224 may be formed on the seating part 220. The fillers 222 and 224 may include a first filler 222 having a uniform size and a second filler 224 having a larger size than the first filler 222, The second pillar 224 contacts the lower surface of the filter 130.

When the first pillar 222 and the second pillar 224 come into contact with the lower surface of the filter 130 to prevent the sample from being formed on the lower surface of the filter 130, As shown in FIG.

Meanwhile, the second pillar 224 having a larger size than the first pillar 222 may be formed to correspond to the position of the pressing portion 118 shown in Fig. Thus, the filter 130 is pressurized both at the top and bottom by the pressurizing portion 118 and the second pillar 224 at the same time, so that a large capillary force is formed, thereby allowing the sample to exit the filter 130 more quickly . Also, the second pillar 224 may be formed in the seating part 220 at a position close to the channel 210 for rapid introduction of the sample into the channel 210.

On the other hand, the length of the second pillar 224 measured in the same direction as the flow direction of the specimen can be formed to be equal to the width of the pressing portion 118. Here, the width of the pressing portion 118 means the sectional width of the pressing portion 118 measured in the same direction as the flow direction of the specimen.

The channel 210 is formed by the combination of the upper plate 100 and the lower plate 200 and the detection unit 240 is formed in the channel 210.

The detection unit 240 includes a detection substance that reacts with the analyte contained in the specimen as the detection target solution. The detection unit 240 may be formed by fixing a coloring reagent such as a fluorescent reagent or a gold reagent on the channel 210. [ For example, the detection unit 240 may include a fluorescence source such as fluorescence or gold nano-beads. When the analyte contained in the sample and the detection substance in the detection unit 240 specifically react with each other, , And these signals can be detected by the naked eye or a detector, or the intensity of light can be measured using a detection system. Therefore, the presence or absence and the amount of the analyte contained in the specimen can be known.

The storage chamber 250 is connected to the channel 210 to store the unreacted residue in the detection unit 240. The reservoir chamber 250 may have a shape that gradually increases in width along the flow direction of the specimen to increase the capillary force and promote the inflow of the residue.

The lower plate 200 further includes a first protrusion 230 formed to be adjacent to the seating part 220 along the flow direction of the specimen and a second protrusion 252 formed in the storage chamber 250 can do.

The first protrusion 230 diffuses the specimen that has passed through the filter 130 in the left and right directions to adjust the flow rate of the specimen and allows the specimen to flow into the channel 210 uniformly. For example, the first protrusion 230 may be formed in an embossed form. The sample moving toward the channel 210 is prevented from proceeding in a linear direction by the first protrusion 230, The channel 210 can be constantly introduced into the channel 210 with respect to the width thereof.

As another example, unlike the drawing, the first protrusion 230 may be a line pattern formed in a direction perpendicular to the flow direction of the specimen. A plurality of line patterns may be formed along the flow direction of the specimen. Such a plurality of line patterns can extend the specimen left and right so that the specimen can flow into the channel 210 uniformly.

The second projection 252 is formed in the reservoir chamber 250 and can prevent the backflow of the residue stored in the reservoir chamber 252. [

6 is a cross-sectional view taken along the line A-A 'of the biosensor of FIG. 1, illustrating a state where the first upper plate 110, the second upper plate 120, and the lower plate 200 are coupled . Hereinafter, the operation of the biosensor 10 according to an embodiment of the present invention will be described with reference to FIGS. 1 to 6. FIG.

First, the specimen is introduced into the filter 130 through the specimen injecting section 114, and the injected specimen flows into the channel 210 through the filter 130. At this time, the filter 130 may perform a function of modifying the characteristics of the specimen. For example, when the sample is blood, blood as a specimen can be separated into plasma through the filter 130.

The filter 130 may be made of a porous film such as paper, nitrocellulose, cellulose, polyvinylidene fluoride (PVDF), poly (ethyleneterephthalate), polyethersulfone (PET), glass fiber or nylon. Not limited.

The upper plate 100 includes a first upper plate 110 positioned on the filter 130 and a second upper plate 120 coupled to the first upper plate 110. Therefore, when the first and second upper plates 110 and 200 are joined to each other, the first plate 110 and the lower plate 200 are twisted due to the height difference caused by the thickness of the filter 130 Thus, leakage of the sample can be prevented.

The first upper plate 110 and the second upper plate 120 include a first protrusion 112 and a second protrusion 122 protruding from the first protrusion 112 and a lower surface of the first protrusion 112, The first upper plate 110 and the second upper plate 120 can be easily coupled to each other. The first protrusion 112 and the second protrusion 122 may be bonded together by a hot-air bonding method, an epoxy bonding method, a chemical bonding method, an ultrasonic bonding method, a plasma bonding method, a solvent bonding method, or the like.

The second protrusion 122 may include an inclined surface 124 and the inclined surface 124 may have a portion 132 extending from one end of the filter 130. The extended portion 132 of the filter 130 may decrease in thickness along the direction in which it extends. The extended portion 132 of the filter 130 is bent upward on the inclined surface 124 to thereby effectively prevent the specimen from leaking from one end of the filter 130. [

On the other hand, the filter 130 is fixed by the pressing portion 118 formed on the first upper plate 110, and is pressed. The lower surface of the filter 130 is in contact with the first pillar 222 and the second pillar 224. The first pillar 222 and the second pillar 224 increase the capillary force and prevent the sample from being formed on the lower surface of the filter 130. Therefore, ). ≪ / RTI >

As described above, the sample passed through the filter 130 is moved toward the channel 210 by the capillary force. However, if there is a large flow resistance between the specimen and the filter 130, the specimen can not move quickly to the channel 210 in the lower part of the filter 130, and a stagnant stagnation section may occur.

In order to prevent this, the first upper plate 110 may be subjected to a hydrophobic surface treatment, and the lower plate 200 and the second upper plate 120 may be subjected to a hydrophilic surface treatment. In particular, May be formed to be larger than the filler (222) and formed to correspond to the position of the pressing portion (118).

6A, the upper surface and the lower surface of the filter 130 are simultaneously pressed by the pressing portion 118 and the second pillar 224, so that a larger capillary force is obtained. As a result, at the portion A, the speed at which the specimen comes out from the filter 130 and the speed at which the specimen moves are increased, and the specimen at the portion A moving at a high speed is stuck together with the stagnant specimen under the filter 130 To the channel 210 side.

Thus, the specimen can quickly exit the filter 130 and enter the channel 210. The second pillar 224 is preferably formed to correspond to the pressing portion 118 formed adjacent to the channel 210 in order to rapidly flow the sample into the channel 210.

The sample introduced into the channel 210 reacts specifically with the detector 240 and the result is detected by the naked eye or a detector or by using a detection system to determine the presence or amount of analytes contained in the sample .

The vent hole 116 may be formed in the first protrusion 112 so that the extended portion 132 of the filter 130 is exposed to atmospheric pressure by the vent hole 116, The sample can flow smoothly.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the invention as defined by the appended claims. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.

10: biosensor 100: upper plate
110: first upper plate 112: first protrusion
114: sample insertion portion 116: vent hole
118: pressing portion 120: second upper plate
122: second protrusion 124: inclined surface
130: Filter 200: Lower plate
210: channel 220:
222: first filler 224: second filler
230: first protrusion 240: detection part
250: storage chamber 252: second projection

Claims (14)

A lower plate including a detection portion;
An upper plate coupled to the lower plate and including a first upper plate and a second upper plate positioned side by side; And
And a filter positioned between the lower plate and the upper plate,
Wherein the first upper plate includes a sample injecting portion formed at a position corresponding to the filter and a first protrusion protruding toward the second upper plate from a side adjacent to the second upper plate,
Wherein the second upper plate includes a second projection projecting toward the first upper plate at a side adjacent to the first upper plate,
Wherein the first upper plate and the second upper plate are coupled by the joining of the first projection and the second projection,
The lower surface of the first projecting portion and the upper surface of the second projecting portion are bonded to each other,
The second protrusion is formed with an inclined surface,
Wherein one end of the filter extends on the inclined surface and is bent upward, and one end of the filter is positioned between the first protrusion and the second protrusion. delete delete delete The method according to claim 1,
Wherein the first upper plate includes a pressing portion protruding from the outer periphery of the sample injecting portion toward the filter. A lower plate including a detection portion;
An upper plate coupled to the lower plate and including a first upper plate and a second upper plate positioned side by side; And
And a filter positioned between the lower plate and the upper plate,
Wherein the first upper plate includes a sample injecting portion formed at a position corresponding to the filter and a first protrusion protruding toward the second upper plate from a side adjacent to the second upper plate,
Wherein the second upper plate includes a second projection projecting toward the first upper plate at a side adjacent to the first upper plate,
Wherein the first upper plate and the second upper plate are coupled by the joining of the first projection and the second projection,
And a vent hole is formed in the first protrusion. A lower plate including a detection portion;
An upper plate coupled to the lower plate and including a first upper plate and a second upper plate positioned side by side; And
And a filter positioned between the lower plate and the upper plate,
Wherein the first upper plate includes a sample injecting portion formed at a position corresponding to the filter and a first protrusion protruding toward the second upper plate from a side adjacent to the second upper plate,
Wherein the second upper plate includes a second projection projecting toward the first upper plate at a side adjacent to the first upper plate,
Wherein the first upper plate and the second upper plate are coupled by the joining of the first projection and the second projection,
Wherein the first upper plate is subjected to a hydrophobic surface treatment, and the second upper plate is subjected to a hydrophilic surface treatment. 6. The method of claim 5,
Wherein the lower plate includes fillers contacting the bottom surface of the filter. 9. The method of claim 8,
Wherein the pillar includes a first pillar and a second pillar larger than the first pillar, and the second pillar corresponds to a position of the pushed portion. An upper plate;
A lower plate engaging with the upper plate; And
And a filter positioned between the upper plate and the lower plate,
Wherein the upper plate includes a first upper plate and a second upper plate coupled and positioned side by side,
Wherein the first upper plate includes a sample injecting unit formed at a position corresponding to the filter and a first protrusion protruding toward the second upper plate at a side adjacent to the second upper plate, And a second projection projecting toward the first upper plate at a surface adjacent to the first upper plate,
Wherein a bottom surface of the first protrusion is joined to an upper surface of the second protrusion, and one end of the filter extends toward the second top plate, and is positioned between the first protrusion and the second protrusion. 11. The method of claim 10,
Wherein the second protrusion is formed on an inclined surface, and one end of the filter is located on the inclined surface. 11. The method of claim 10,
Wherein the first upper plate includes a pressing portion protruding from the outer periphery of the sample injecting portion toward the filter. 13. The method of claim 12,
The lower plate comprising fillers in contact with the bottom surface of the filter,
Wherein the fillers include a first filler and a second filler larger than the first filler, and the second filler corresponds to the position of the pressing portion. 11. The method of claim 10,
Wherein the first upper plate is subjected to a hydrophobic surface treatment, and the second upper plate and the lower plate are subjected to a hydrophilic surface treatment.

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