KR101839918B1 - Method of Sterilizing and Promoting Germination of Seed Using Non-thermal Plasma - Google Patents

Abstract

The present invention relates to a method of sterilizing seeds and promoting germination using a non-thermal plasma. According to the present invention, by subjecting a seed surface such as a sprout seed to a direct heat plasma treatment, the microbes existing on the surface of the sprout can be sterilized, The germination rate and the germination rate of the seeds can be shortened to shorten the time required for germination of the seeds, thereby shortening the period of time required for the production of agricultural and sprouting vegetables. Thus, safer foods can be provided.

Description

Technical Field [0001] The present invention relates to a method of sterilizing seeds using non-thermal plasma,

The present invention relates to a method of sterilizing seeds and promoting germination using a non-thermal plasma, and more particularly, to a method of sterilizing seeds using non-thermal plasma which directly treats non-thermal plasma on a seed surface to sterilize microorganisms on the surface and accelerate seed germination rate and germination rate. And a method of promoting sterilization and germination of seeds.

Plant seeds are a means of propagation of plants, one of the important food crops of mankind. Seeds enable the growth of plants and species conservation. These seeds germinate by absorbing water in a growth-favorable environment and transform into plants.

Plant seeds are the largest proportion of human food, and are used as seeds or after germination for food. Vegetables have been the mainstream for cultivated food, but recently, various functional sprouts have been attracting attention as health foods.

Sprout vegetables are foods that require special attention in terms of food hygiene, since they are consumed as they are without going through a separate disinfection process. In fact, food poisoning accidents caused by sprouting vegetables are frequent in many parts of the world and account for more than 40% of the foods causing food poisoning accidents in the US, UK, Canada, and Japan.

Based on the results of the Center for Disease Control and Prevention (CDC) in 2009, the Center for Science in the Public Interest (CSPI) of the United States has identified 10 dangerous food groups I have announced that, among them, sprouts vegetables are included. In fact, it was reported that 31 spores were caused by 2,022 patients.

To produce hygienic sprout vegetables, seed sterilization is essential prior to a clean cultivation environment. Seed disinfection is usually carried out using a disinfectant, but the sprout vegetable ingested immediately after germination can not be used because of the high residual risk.

On the other hand, the plasma referred to as the ionized gas is a plasma in which ions and electrons are distributed at similar densities and contain ultraviolet rays generated during transition of ions together with many excited ions, free radicals, electrons, photons, It shows sterilization effect.

Plasma is composed of electrons with negative electric charge and ions with positive electric charge by discharging in gas, and the charge and ion are highly separated, but they have neutrality because they have the same charge density and ion density. As the gas receives high energy and separates into charge and ion, it becomes a plasma state. It absorbs energy and generates a large amount of excited ions whose energy level of the orbit electron rises. These excited ions, or ultraviolet rays generated by excited ions, affect microbial killing. Plasma is distinguished by a high-temperature plasma and a low-temperature plasma. Due to the nature of foods that are susceptible to heat, only cold (cold) plasma can be applied to food.

The cold plasma may be generated by a radio-frequency plasma (RFP), a microwave discharge plasma (MDP), a dielectric barrier discharge plasma (DBDP), a corona discharge plasma , CDP), and gliding arc discharge plasma (GADP). The cold plasma that can be generated most simply is the corona discharge plasma. However, the corona discharge plasma can be applied to foods only when a method of lowering the temperature is required because the temperature of the corona discharge plasma is higher than that of other types of cold plasma. The corona discharge plasma jet (CDPJ), which lowers the temperature of the plasma and ejects the plasma in the form of a jet while supplying a large amount of air between the electrodes while generating plasma by lowering the temperature of the corona discharge plasma, Is low and is easy to operate and can be applied to various types of object.

Korean Patent Publication No. 1998-068414 (Oct. 15, 1998) and US Patent No. 8,896,211B2 (Nov. 25, 2014) disclose an apparatus for promoting plant growth by treating low temperature plasma to plant seeds Korean Patent Laid-Open Publication No. 2014-0063211 (Apr. 26, 2014) discloses a method for decomposing and removing residual agricultural chemical components of agricultural products using a low-temperature plasma jet such as a corona discharge plasma jet (CDPJ).

In addition, the inventor's prior article (Yoon, Keun-A, Mok, Chul-gyun, Journal of the Korean Society of Food Science and Technology v.47, No. 1, pp. 239 (February 2015) pp. 70-74; (August 2013) pp. 266-270) have studied the microbial or microbial effect of E. coli on the surface of cereals using a corona discharge plasma jet (CDJP).

On the other hand, germination is facilitated because the dormancy period after seeding is reduced and yields can be produced with less production time. In order to increase the germination rate of seeds, there are various methods of controlling the dormancy by controlling the dormancy before germination. Among them, the plant growth regulators are relatively used because they can confirm the effect of increasing the germination rate at a relatively low treatment cost. However, plant growth regulators are difficult to apply because they are known to have effects on selective crop action and conditions.

Therefore, the present inventors have found that, instead of the plant growth regulator having a problem that the effect varies depending on the selective crop action and the condition, the seed surface is sterilized by non-thermal plasma treatment such as corona discharge plasma jet (CDPJ) And when germination is promoted, the microorganisms remaining on the seed surface are sterilized, thereby securing safety against microorganisms at germination, promoting seed germination while providing stability without changing quality after seed germination, and thereby improving the safety and economy of sprouts vegetables The present invention has been completed.

Korean Patent Publication No. 1998-068414 (Oct. 15, 1998) U.S. Patent No. 8,896,211 B2 (November 25, 2014). Korean Patent Publication No. 2014-0063211 (April 27, 2014).

It is an object of the present invention to provide a method of disinfecting the seed surface and promoting germination of the seed by directly spraying a non-thermal plasma on the seed surface.

In order to achieve the above object, the present invention provides a method for sterilizing seeds and promoting germination, which comprises subjecting seed surfaces to specific heat plasma treatment.

The non-thermal plasma may be a corona discharge plasma jet (CDPJ).

It is possible to perform the non-thermal plasma treatment with any one value selected from the range of 1 second to 3 minutes.

Plasma spraying can be performed at a frequency of 25 to 50 kHz at a current density of 1.0 to 1.55 A and at a distance of 15 to 75 mm from the plasma discharge port.

The non-thermal plasma can be subjected to three-dimensional injection processing.

The seed may be a sprout vegetable seed.

The sprouts may be selected from the group consisting of oilseed rape, broccoli, non-walnut, and green walnut.

The method for promoting sterilization and germination of seeds according to the present invention is a method for promoting germination of seeds by promoting germination of sprouts and seeds by promoting the safety of microorganisms on germination by treating the microorganisms remaining on the surface, The quality can be improved.

1 is a graph showing a sterilization pattern of a surface of a germ seed by a corona discharge plasma jet (CDPJ) treatment according to an embodiment of the present invention.
FIGS. 2A to 2D are graphs showing growth patterns of microorganisms on a surface of a seed of a seed by a corona discharge plasma jet (CDPJ) treatment according to an embodiment of the present invention. FIG.
FIG. 3 is a graph showing seed germination results with time according to a corona discharge plasma jet (CDPJ) treatment according to an embodiment of the present invention.
FIG. 4 is a graph showing changes in weight of seeds with time according to a corona discharge plasma jet (CDPJ) treatment according to an embodiment of the present invention.
FIG. 5 is a graph showing changes in growth length of seeds with time according to a corona discharge plasma jet (CDPJ) treatment according to an embodiment of the present invention.
FIG. 6 is a graph comparing the numbers of seeds after germination by corona discharge plasma jet (CDPJ) treatment according to an embodiment of the present invention.
7 is a graph showing germination rates of seeds (a: oilseed, b: broccoli, c: non-conspicuous) in the corona discharge plasma jet treatment according to an embodiment of the present invention with distance from the plasma discharge port.
8 is a graph showing a sterilization rate of a seed (a: oilseed, b: broccoli, c: unsorted, d: blueberry) according to distance from the plasma discharge port in the corona discharge plasma jet treatment according to an embodiment of the present invention.
9 is a cross-sectional view and a photograph showing an outline of a corona discharge plasma jet apparatus used in an embodiment of the present invention.
10 is a cross-sectional view schematically showing an apparatus for sprouting germs used in an embodiment of the present invention.

In the present invention, when the seed surface is treated with a non-thermal plasma such as a corona discharge plasma jet (CDPJ) to sterilize and germinate the seed, the microorganisms remaining on the seed surface are sterilized, And to confirm that seed germination can be promoted while providing stability without changing quality after seed germination.

Therefore, the present invention relates to a method of sterilizing seeds and promoting germination, characterized by subjecting seed surface to specific heat plasma treatment in one aspect.

In the present invention, by optimizing the characteristics and conditions of the nonthermal plasma used in the seed germination promoting method, seeds can be sterilized and seed germination can be promoted efficiently.

The non-thermal plasma treatment used in the seed germination promoting method of the present invention can be preferably a corona discharge plasma jet (CDPJ) treatment. The corona discharge plasma jet (CDPJ) treatment is carried out between narrow electrodes A plasma can be generated by applying a high frequency current and then injected at a high speed to emit a jetless type of non-thermal plasma at the same time as air cooling. This method is simple in control and capable of moving plasma And at the same time, the action of the plasma can be enhanced by increasing the contact between the active particles and the object by the stirring effect by the gas.

The non-thermal plasma treatment according to the present invention can be treated within 2 minutes, and preferably for 0.5 to 2 minutes. If the treatment is performed for more than 2 minutes, the germination proceeds slowly, which is similar to the weight of seeds that have not germinated, and the germination rate is lowered. Therefore, it can be seen that the germination of the sprout seeds is the longest and grows rapidly when treated for less than 2 minutes, which is effective for hygiene improvement without affecting the sensory quality, and also has a positive effect on the growth by shortening the germination time You can give.

The nonthermal plasma treatment according to the present invention may also be applied to the case where the current intensity is 1.0 to 1.55 A applied at a frequency of 25 to 50 kHz and the distance is 15 to 75 mm, preferably 20 to 35 mm, more preferably 20 to 30 mm The spraying process can be performed under the condition set at. If the distance from the discharge port is less than 15 mm, seed deformation due to deterioration affecting the germination of the seed may occur, which is undesirable. In the case of the non-thermal plasma treatment under the above conditions, the germicidal effect of the microorganisms existing on the surface of the bud is increased, and the germination rate and the germination rate of the germination seed are promoted.

Further, the corona discharge plasma jet (CDPJ) can preferably be sprayed in three dimensions.

The seed of the present invention is preferably a sprout vegetable seed, and may be applied to a sprout seed such as rapeseed seed, broccoli seed, a seedless seed, a seedless seed, etc. However, the present invention is not limited thereto, And can be directly applied to the seed surface as it is.

As a result of observing DPPH radical scavenging ability and antioxidative ability by the nonthermal plasma treatment of the present invention as described above, the germination promoting method of the present invention is effective for promoting germination of seed without changing quality before and after plasma treatment. do.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these examples are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.

[Example]

Manufacturing example  1: corona discharge plasma  Z( CDPJ ) Produce

Corona Discharge Plasma Jet (CDPJ) uses a pair of electrodes in a ceramic shield (shield) and applies a voltage of 1.0-20.0 kV pulsed DC between electrodes while introducing a large amount of air using a blower to generate a plasma And the generated plasma is injected to the lower portion of the electrode using the blowing pressure.

A corona discharge plasma jet (CDPJ) treatment apparatus is shown in Fig. Specifically, the corona discharge plasma jet (CDPJ) treatment system consists of four parts: a voltage / high frequency power supply, a blower, a plasma discharge part, and a sample transfer system. At this time, 220 V AC was used as the power source, and a pulse type square wave was generated by varying the frequency in the range of 10-58 kHz. The output voltage is DC 1-20 kV, the current is changed according to the frequency, and the maximum power consumption is 2 kW. The tungsten lead having a diameter of 3 mm and a length of 20 mm was arranged in parallel with the discharge part, and the discharge part was embedded in a Teflon shield having a thickness of 5 mm. The plasma was discharged by injecting air at an air velocity of 2.5 m / jet type plasma was released. Sliding gears (or ball bearing gears) are mounted on the plasma processing table to enable linear movement.

In the operation of the CDPJ treatment apparatus, first, the fan is operated to supply air at a stable wind speed, then a voltage is applied to generate plasma by corona discharge, and the generated plasma forms a jet by the wind pressure.

Example  1-3: For sprouting plasma  process

The seed sprouts as shown in Table 1 were prepared in a Petri dish, and then the corona discharge plasma jet (CDPJ) produced in Production Example 1 was treated for 1 minute and 3 minutes.

The external shape and functional configuration of the corona discharge plasma jet apparatus are shown in Fig. 9, and the external shape and functional configuration of the germination apparatus are shown in Fig.

The construction and operation of the non-thermal plasma jet device using the corona discharge are described in Korean Patent Registration No. 10-1471854 (Apr. 12, 2014), which was previously filed and registered as "the residual pesticide component Decomposition and removal method 'of the present invention, and redundant explanation will be omitted because the corresponding configuration is used as it is.

On the other hand, the germination apparatus shown in FIG. 10 has a cylindrical shape as a whole and has a vertical length of 35 to 50 centimeters in height, a relatively preferable diameter of 43 cm, a diameter of 15 to 25 cm, The size of the diameter may vary from part to part. The lower water tank is a water tank with a capacity of about 4 liters. It has a water pump that operates to input 220 volts and 60 Hz power. The water is pumped through the water transfer pipe to the upper cover, And is generally cylindrical in shape and has a diameter ranging from 15 to 20 cm and preferably has a diameter of 20 cm. The growth portion located at the middle portion includes a wicker tray, a region through which a water transfer pipe of a water pump passes, a variety of seeds are arranged and grown, a diameter ranging from 18 to 23 cm, and a diameter of 23 cm Is relatively preferable. The upper cover part is provided with a water transmission pipe extended and a spin nozzle is installed at the end of the water transmission pipe to discharge the water supplied by the water tank in a scattered state and has a diameter ranging from 21 to 25 cm, cm < / RTI > in diameter. A filter portion made of a nonwoven fabric may be formed at a lower end portion of the cover portion in order to block the passage of foreign matter contained in the water discharged from the cover portion in a scattered state, and the nonwoven fabric may be configured to block foreign matter having a size of 0.1 mm or more from passing therethrough ≪ / RTI >

At this time, the corona discharge plasma jet (CDPJ) was obtained under conditions set at a current intensity of 1.5 A applied at a frequency of 25 kHz and a distance of 25 mm from the plasma discharge port.

division Korean name Scientific name English name Example 1 Rapeseed Brassicanapus napus L. Rapeseed Example 2 broccoli Brassica There is oleracea . kialica plen. Broccoli Example 3 Random Raphanussativus saltivus L. Young radish Example 4 Cheonggyeongae Brassica campestris L. ssp. ch Bok choy

<Experimental Example>

Experimental Example  1: corona discharge plasma  Z( CDPJ ) Disinfection effect by treatment

1. General characteristics of sprout seeds

As a result of the pollution degree of the sprout seed purchased, the pollution degree as shown in Table 2 was shown.

Bud name microbe Log (CFU / g) Rapeseed Aerobic bacteria 8.46 ± 0.05 Bacillus 7.04 ± 0.17 mold 6.19 ± 0.06 Coliform group 6.09 ± 0.13 Salmonella 5.33 ± 0.20 Staphylococcus N / D Listeria N / D broccoli Aerobic bacteria 8.52 ± 0.18 Bacillus 7.38 ± 0.23 mold 7.18 ± 0.13 Coliform group 5.88 ± 0.05 Salmonella 4.90 ± 0.09 Staphylococcus N / D Listeria N / D Random Aerobic bacteria 7.99 + 0.04 Bacillus 6.92 + 0.03 mold 6.60 + 0.08 Coliform group 6.12 ± 0.07 Salmonella 5.81 ± 0.03 Staphylococcus N / D Listeria N / D Cheonggyeongae Aerobic bacteria 8.15 ± 0.05 Bacillus 6.98 ± 0.05 mold 6.72 + 0.03 Coliform group 6.36 ± 0.07 Salmonella 5.91 + 0.02 Staphylococcus N / D Listeria N / D

In 2009, the CSPI (Center for Science in the Public Interest) included sprouting vegetables as one of the 10 dangerous foods that frequently cause food poisoning in the United States since 1990, and 31 cases of food poisoning caused by sprouting vegetables And the number of patients was 2,022.

In the case of foods with freshness of life, when the microorganisms are found at 7-8 log CFU / g or more, the value of the product drops greatly and the consumer does not receive the trust. In addition, E. coli was detected in 97% of fresh vegetables, Staphylococcus was detected in 17.7 ~ 24%, Salmonella was detected in 3.3%, and Listeria was detected in 0.3%.

2. Corona Discharge plasma  Sterilization effect of sprout seeds by treatment

FIG. 1 shows a microbial reduction graph of the sprout seeds (oilseed rape, broccoli, silkworm, and bluegrass) according to the corona discharge plasma treatment. As the plasma treatment time continued, the number of aerobic bacteria, Bacillus , fungi, E. coli and Salmonella microorganisms continued to decrease, and it was confirmed that the number of microorganisms decreased by more than 2 log in 3 minutes treatment.

The D values of the sprouted seeds treated with corona discharge plasma are shown in Table 3.

Bud name microbe D-value ^* (minutes) Rapeseed Aerobic bacteria 1.17 Bacillus 2.30 mold 1.49 Coliform group 1.29 Salmonella 1.44 broccoli Aerobic bacteria 1.09 Bacillus 2.20 mold 1.59 Coliform group 1.20 Salmonella 1.36 Random Aerobic bacteria 1.30 Bacillus 2.26 mold 1.62 Coliform group 1.30 Salmonella 1.58 Cheonggyeongae Aerobic bacteria 1.21 Bacillus 2.45 mold 1.69 Coliform group 1.39 Salmonella 1.69

* D value is an abbreviation of decimal reduction value, which means the time required to reduce the number of microorganisms in the seed by 1, or 90%, in log cycle.

As shown in Table 3 and Fig. 1, it is shown that the aerobic bacteria, Bacillus , fungus, coliform and Salmonella can be reduced by 90% within 1 to 2.5 minutes on the surfaces of rape seed, broccoli seed, Respectively.

As shown in Figs. 2 (a) to 2 (d), as the germination time increases, the rising curve of the growth rate of aerobic bacteria, Bacillus , fungi, E. coli and Salmonella in each sprout seeds slows down as the plasma treatment time increases .

Further, as shown in Fig. 6, in the bactericidal effect of aerobic bacteria, fungi and Escherichia , it was found that the above-mentioned bacteria were considerably killed as compared with the case where the seed surface was subjected to non-thermal plasma treatment for 2 minutes .

Experimental Example  2: corona discharge plasma  Effect of treatment on the growth of buds

In order to investigate the effect of corona discharge plasma treatment on the growth of buds, we measured the weight, length and germination rate of buds according to plasma treatment time.

The germination rate of the plasma treated broccoli sprout seeds was expressed as the ratio of the number of germinated seeds to the number of seeds sown after 5 days of growing the sprouts. As shown in FIG. 3, the germination rate of the seeds sprayed with plasma for 1 minute was the best, and that of the seeds treated for 3 minutes was rather lower than the germination rate.

Seeds become dormant before germination, which is a natural attribute of the seed to preserve the race, and cold plasma is known to be able to stimulate the dormancy of this seed. Plasma processing produces ultraviolet, active oxygen, and the resulting chemical reaction affects the dormancy. Cold plasma treatment has been reported to improve the growth of wheat, oats, and radish buds. However, the difference in the effect of plasma treatment with different output power was shown. Low energy plasma treatments did not affect the seeds, and high energy plasma treatments could have too much effect on the seeds. This result indicates that the optimum treatment time is required for the plasma treatment, and the optimum time for the treatment of the bud is experimentally confirmed in the range of 1 minute to 2 minutes.

The weights of the sprouts were measured using a scale and the lengths were measured with vernier calipers. As shown in Fig. 4, the weight was the highest in the seeds treated for 2 minutes, and the seeds weighed in the order of 1 minute and 0 minutes (without plasma treatment). The seeds treated for 3 minutes were seeds which did not germinate . As shown in FIG. 5, the length of the germinated seeds treated for 2 minutes was the longest and fastest at the time of germination, and the germination was very slow at the seeds treated for 3 minutes.

Experimental Example  3: corona discharge plasma  Changes in quality of buds after treatment

Table 4 shows the moisture content, reducing sugar, total phenol content and DPPH radical scavenging ability of the buds treated with different corona discharge plasma treatment times.

Bud name CDPJ processing time
(minute) Physical and chemical properties Water content (%) Reducing sugar content (%) Total phenol content (μg GAL / g) DPPH radical scavenging ability (%) Rapeseed 0 87.01 + 4.93 a 0.78 ± 0.05 a 14.16 ± 0.33 a 43.19 + 1.23 a One 85.38 ± 2.96 a 0.80 + 0.07 a 13.86 ± 0.51 a 43.46 ± 1.58 a 2 85.14 ± 2.85 a 0.78 + 0.04 a 14.03 ± 0.58 a 43.01 + 1.37 a 3 81.52 ± 5.31 a 0.79 + 0.03 a 13.86 ± 1.09 a 43.22 ± 1.40 a broccoli 0 94.71 ± 1.56 a 0.55 + 0.01 a 11.76 ± 0.86 a 69.77 ± 6.01 a One 94.72 ± 1.15 a 0.54 0.03 a 11.94 ± 0.86 a 68.83 ± 3.83 a 2 94.07 ± 1.74 a 0.54 + 0.04 a 11.71 ± 0.76 a 69.77 ± 5.15 a 3 94.78 +/- 0.47 a 0.55 0.03 a 11.99 ± 0.97 a 65.82 ± 3.73 a Random 0 88.12 + 4.63 a 0.55 0.02 a 12.37 ± 0.72 a 78.03 ± 3.76 a One 86.46 ± 2.19 a 0.55 + 0.04 a 12.40 ± 0.87 a 78.47 ± 3.49 a 2 88.70 ± 3.86 a 0.55 0.02 a 12.27 ± 0.81 a 78.34 + - 6.41 a 3 86.35 + 4.46 a 0.55 + 0.01 a 12.58 ± 0.89 a 78.60 + - 4.31 a Cheonggyeongae 0 86.04 + 1.55 a 0.77 ± 0.05 a 11.39 ± 1.09 a 64.78 ± 3.86 a One 87.51 ± 0.85 a 0.77 ± 0.05 a 11.90 ± 0.97 a 62.63 + 4.59 a 2 85.45 ± 3.65 a 0.77 ± 0.08 a 11.70 ± 1.14 a 63.31 ± 3.80 a 3 87.47 ± 1.08 a 0.78 + 0.01 a 11.40 ± 1.21 a 63.27 ± 3.99 a

As shown in Table 4, the moisture content, reducing sugar, total phenol content, and DPPH radical scavenging ability of rapeseed, broccoli, non-seeded, and seedless seeds were not significantly changed with increasing corona discharge plasma treatment time.

As a result of measuring the moisture content, reducing sugar, total phenol content and DPPH radical scavenging ability by the nonthermal plasma treatment of the present invention, the germination promoting method of the present invention was found to be effective for promoting germination of the seed before and after the plasma treatment, It is experimentally confirmed that it has an effective action.

Experimental Example  4: Corona discharge plasma  Effect of treatment on the sensory characteristics of buds

The results of the sensory evaluation of the sprouted seeds treated with corona discharge plasma are shown in Table 5.

Bud name characteristic Processing time (minutes) ²⁾ 0 One 2 3 Rapeseed Exterior 8.08 a 8.17 a 8.25 a 2.92 b color 8.17 a 8.00 a 8.33 a 8.25 a incense 5.58 a 5.83 a 5.67 a 4.92 b flavor 5.25 a 5.33 a 5.75 a 2.00 b Texture 6.17 a 6.67 a 6.17 a 6.25 a Overall likelihood 6.67 a 7.00 a 7.08 a 5.58 b broccoli Exterior 8.25 a 8.08 a 8.33 a 2.75 b color 8.08 a 8.08 a 8.17 a 5.75 b incense 6.08 b 6.33 b 6.25 b 7.33 a flavor 6.17 a 6.25 a 6.08 a 2.08 b Texture 6.75 b 6.67 b 6.75 b 8.75 a Overall likelihood 7.58 a 7.67 a 7.50 a 2.42 b Random Exterior 8.08 a 8.42 a 8.25 a 2.83 b color 8.00 a 7.83 a 8.58 a 5.83 b incense 6.00 b 6.42 b 6.50 b 7.25 a flavor 6.08 a 6.33 a 6.17 a 2.17 b Texture 6.83 a 6.75 a 7.00 a 6.75 a Overall likelihood 7.50 a 7.75 a 7.92 a 2.50 b Cheonggyeongae Exterior 7.50 a 7.67 a 7.92 a 2.42 b color 7.75 a 7.67 a 7.83 a 7.17 a incense 6.58 a 6.67 a 6.50 a 6.83 a flavor 5.83 a 6.00 a 5.83 a 1.92 b Texture 6.42 b 6.42 b 6.50 b 8.42 a Overall likelihood 7.25 a 7.42 a 7.08 a 2.08 b ¹⁾ Twelve students were graded on a 9-point scoring system.
²⁾ Significant difference was found in the same row using significance test (p <0.05).

There was no statistically significant difference between the treatments within 2 minutes and the control. However, it was found that there was a significant difference in the sprouts cultivated after 3 minutes treatment, and it was confirmed that processing within 2 minutes did not affect the sensory quality. It is expected that the proper adjustment of the treatment time of the corona discharge plasma is effective for improving hygiene without affecting the sensory quality, and will have a positive impact on the growth by shortening the germination time, so that seed technology will be influential in the future.

Experimental Example  5: Corona Discharge plasma  Effect of treatment distance on germination and sterilization rate of bud

In the corona discharge plasma jet treatment, when the distance from the plasma discharge port was 15 mm, 25 mm, 35 mm and 45 mm, the germination rate and the sterilization rate of the seed were confirmed and shown in FIG. 7 and FIG.

7 is a graph showing the germination rate per seed, wherein 7a is rapeseed ( Brassicanapus napus L., rapeseed), 7b is broccoli ( Brassica There is oleracea . kialica plen., broccoli), 7c is Raphanussativus saltivus L., young radish, 7d is Brassica campestris L. ssp. ch, bok choy) germination rate.

As shown in FIGS. 7A to 7D, the germination rate was remarkably increased as the distance between each seed and the plasma discharge outlet increased from 15 mm to 25 mm. However, when the distance increased to 35 mm, the germination rate was slightly decreased. The results are similar to those of non - treatment. In addition, the germination rate decreased in the proximity treatment of less than 15 mm.

8 (a) to 8 (d), 8a is rapeseed ( Brassica napus napus L., rapeseed), 8b is broccoli ( Brassica oleracea var. Kialica plen., Broccoli) , 8c is Raphanussativus saltivus L., young radish, and 8d is a graph showing the sterilization rate of Brassica campestris L. ssp. Ch. Bok choy.

As shown in FIGS. 8A to 8D, as the distance between each seed and the plasma discharge outlet increased from 15 mm (I) to 25 mm (II), the sterilization rate was greatly increased and increased to 35 mm (III) In the case of the case, the result showed a slight decrease.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (7)

In the nonthermal plasma treatment on the seed surface
The non-thermal plasma is a corona discharge plasma jet (CDPJ)
The non-thermal plasma treatment is a three-dimensional injection treatment at a distance of 15-50 mm from the plasma discharge opening at a current intensity of 1.0 to 1.55 A at a frequency of 25 to 50 kHz with a value selected from the range of 30 seconds to 2 minutes,
Wherein the seed is a sprout vegetable seed selected from the group consisting of oilseed rape, broccoli, non-walnut, and green walnut seeds.
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