KR101777368B1 - Composition for skin-whitener containing extract of china rose - Google Patents

Abstract

The present invention relates to a skin whitening cosmetic composition containing an extract of Laureliaceae as an active ingredient and can be usefully used for skin whitening because it inhibits the production of melanin pigment and suppresses expression of tyrosinase gene and exhibits excellent inhibitory effect on pigment deposition .

Description

[0001] The present invention relates to a skin whitening cosmetic composition containing an extract of Laurel onion as an active ingredient,

The present invention relates to a skin whitening cosmetic composition containing as an active ingredient an extract of Laurel on White which can be usefully used for skin whitening, and a pharmaceutical composition for treating and preventing skin pigmentation diseases.

Melanin pigment absorbs ultraviolet rays and protects skin organs from damage caused by ultraviolet rays. It protects skin by removing harmful substances such as harmful oxygen and free radicals generated in skin .

Despite the useful role of the melanin pigment, when melanin pigment is overly synthesized by various environmental factors such as ultraviolet ray, hormone change, inflammation, or medicines and is deposited on the skin, it causes skin pigmentation disease and adversely affects skin beauty And, furthermore, it causes skin cancer.

Skin pigmentation disease caused by melanin pigment is recognized as a factor that hinders whitening, which is a standard of modern beauty, and various solutions are being studied in terms of health as well as cosmetics.

Currently, skin pigmentation treatment methods include hydroquinone bleach, vitamin A ointment, topical steroid ointment, vitamin C, glycolic acid, and azelaic acid, while using a mixture of high concentrations of vitamin C and electrophoresis . Recently, TCA or glyceral acid - based dressings have been used in many cases with local treatment.

However, until now, there is no perfect way to treat skin pigmentation disease, and there is a possibility of recurrence easily with any treatment method. Therefore, it is required to develop a new therapeutic agent capable of inhibiting the production of melanin pigment based on the fundamental understanding of the mechanism of skin melanogenesis.

On the other hand, the melanin pigment is converted from tyrosinase (DOPA) to dopaquinone (DOPA quinone) by an enzyme called tyrosinase in the melanocyte endoplasmic reticulum in the melanocyte cell, It is synthesized through DOPA chrome through oxidation and enzymatic reaction.

The synthesized melanin pigment is transferred and distributed to the surrounding keratinocyte and becomes a phenotype called dark brown pigmentation or skin pigmentation disease in the skin.

Therefore, inhibiting the synthesis of melanin pigment is the most fundamental method for treating and preventing skin pigmentation disease, and it is a toxic substance specifically acting on melanocyte cells synthesizing the melanin pigment or melanin pigment metabolism Studies have been actively conducted to achieve skin whitening and skin pigmentation diseases by regulating the expression of tyrosinase gene, which is involved in the synthesis process.

P-methoxyphenol, hydroquinone, kojic acid, arbutin, etc. have been used to treat skin pigmentation diseases so far, but they are weakly active It may cause denaturation or lethality of the pigment cells, and the original function of the cells is impaired.

On the other hand, vitamin C and its derivatives and the like have been used for the purpose of inhibiting melanin production, but these also have a disadvantage of low inhibitory activity.

In addition to these therapeutic agents, research on finding whitening active ingredients in natural products, especially plants, has been continued. Among them, many plant extracts and herbal medicine extracts such as alfalfa, licorice, peony, cinnamon, Have been found to act on tyrosinase to inhibit melanin production. However, these have also been found to have a number of problems in terms of their safety from being used in cosmetics or medicines in excess of the effective concentration, have.

Accordingly, it is urgently required to develop natural products having a small amount of melanin biosynthesis inhibiting activity and a small side effect.

Korean Patent No. 0917281 Korean Patent No. 0831092

It is an object of the present invention to provide a skin whitening cosmetic composition containing an extract of Wolgyering, which can be usefully used for skin whitening, as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for the treatment and prevention of skin pigmentation diseases, which comprises an extract of Wallaceae as an active ingredient.

In order to attain the above object, the skin whitening cosmetic composition of the present invention may contain an extract of Wolgyering, as an active ingredient.

The Walukae extract may be an extract obtained by extracting petals and stem of Walukae flower.

The wollatwaal extract may be obtained by extracting a mixture of petal and stem of Wolgye-gae with a weight ratio of 1: 0.2 to 0.9.

The Walukae extract may be a hot-water extract at 80 to 120 ° C for 0.5 to 3 hours.

The skin whitening cosmetic composition may be used as a skin cosmetic composition for skin such as a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, , A cleansing lotion, a cleansing cream, a body lotion, a body cleanser, an emulsion, a press powder, a loose powder, and an eye shadow.

The skin whitening can be carried out by inhibiting the production of melanin pigment, and the skin whitening can also be carried out by inhibiting tyrosinase gene expression.

According to another aspect of the present invention, there is provided a pharmaceutical composition for the treatment and prevention of skin pigmentation diseases, which comprises an extract of Wolgyunggae as an active ingredient.

The skin pigmentation disease occurs locally in the skin due to increased synthesis of melanin pigment and is selected from hyperpigmentation caused by spots, freckles, black spots, nevi, drug-induced pigmentation, post-inflammatory pigmentation, and dermatitis It may be one or more diseases.

The wartime extract may be contained in an amount of 0.001 to 99.9% by weight based on the total weight of the composition.

The skin whitening cosmetic composition of the present invention can effectively inhibit the production of melanin pigment by inhibiting the synthesis of tyrosinase involved in melanin pigment formation by using an extract obtained from a mixture of rosemary extract, especially a mixture of rosemary flower petal and stem. Accordingly, the skin whitening cosmetic composition of the present invention has an excellent skin whitening effect by inhibiting the formation of melanin pigment, and thus can be effectively used as a skin whitening cosmetic composition.

In addition, the skin whitening cosmetic composition of the present invention can be effectively used for treating and preventing skin pigmentation diseases caused by overproduction of melanin pigment by inhibiting the formation of melanin pigment.

The present invention relates to a skin whitening cosmetic composition containing as an active ingredient an extract of Laurel on White which can be usefully used for skin whitening, and a pharmaceutical composition for treating and preventing skin pigmentation diseases.

The above-mentioned "skin whitening" means inhibiting or preventing skin deposition of melanin pigment by inhibiting synthesis of melanin pigment, and is used to include treatment or prevention of skin pigmentation disease.

The above-mentioned "skin pigmentation disease" includes symptoms such as spots, freckles, black spots and nevi occurring locally in the skin due to an increase in synthesis of melanin pigment, pigmentation caused by drugs, Or hyperpigmentation after inflammation, or hyperpigmentation, which is a symptom of dermatitis.

Hereinafter, the present invention will be described in detail.

The skin whitening cosmetic composition of the present invention contains an extract of Laureliaceae as an active ingredient.

In order to prepare the ugly extract, firstly, it is washed with water to remove the moisture remaining in the petals and stems of the rosemary which have been removed from the debris, and freeze-dried in the freeze dryer to be powdered.

The stem is a mixture of branches and leaves attached to the branches in a weight ratio of 1: 1 to 2. When the content of the leaf is less than the lower limit based on the branch, the whitening effect can not be exhibited when mixed with the petal. If the content is less than the lower limit, toxicity may be caused.

The extract of Walukae extract of the present invention can be excellently whitened by using an extract of petal powder or a mixture of petal powder and stem powder. However, the whitening effect can not be expected at all when the extract is prepared using only the stem powder.

The mixed powder is obtained by mixing the petal powder and the stem powder at a weight ratio of 1: 0.2 to 0.9, preferably 1: 0.4 to 0.6. When the content of stems on the basis of the petals is less than the lower limit value, an excellent whitening effect can not be expected, and when the content is above the upper limit value, whitening effect can not be expected at all.

Next, the above-described launderability powder and water are mixed at a weight ratio of 1: 8 to 12, preferably 1:10 to 12, at 80 to 120 ° C, preferably 100 to 120 ° C for 0.5 to 3 hours, Is subjected to hot water extraction for 1.5 to 2 hours.

When a lower alcohol having 1 to 4 carbon atoms other than water is used as the extraction solvent, the production of melanin pigment may not be suppressed even at a low concentration as well as at a high concentration.

When the weight of the pomegranate powder and water is out of the above weight ratio, the effective amount of pomegranate may be extracted in a small amount.

When the extraction temperature is lower than the lower limit, the production of melanin pigment may not be suppressed at all. If the extraction temperature is above the upper limit, the production of melanin pigment can not be inhibited at a concentration of 100 ug / ml or lower, It is possible to suppress the production of melanin pigment which is slightly reduced by about 5% as compared with cialumin.

In addition, when the extraction time is less than the lower limit value, the effective amount of the laurel treatment can be extracted in a small amount. If the extraction time exceeds the upper limit value, the production of melanin pigment can not be inhibited and toxicity can be caused.

The Walukehae extract prepared as described above may be used as an extract itself or may be pulverized for convenient use. In the method of pulverizing the mulberry flower extract, the extract is concentrated under reduced pressure to reduce its volume, and then lyophilized by a freeze dryer to be pulverized.

The skin whitening cosmetic composition of the present invention may be blended with other components which are usually added to cosmetics, if necessary, in addition to the above-mentioned wattled calli extract, which is an essential ingredient. Examples of such blending ingredients include a moisturizing agent, an emollient agent, a surfactant, And an inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an alcohol, a pigment, a flavor, a blood circulation promoter, a cold agent, a limiting agent and purified water.

The skin whitening cosmetic composition may be prepared in the form of an emulsified formulation and a solubilized formulation commonly used in the art, and examples thereof include a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, Nutrition Creams, Moisture Creams, Hand Creams, Essences, Nutritional Essences, Packs, Soaps, Shampoos, Cleansing Foams, Cleansing Lotions, Cleansing Creams, Body Lotions, Body Cleansers, Milk, Press Powder, Loose Powder, or Eye Shadow have.

In addition, the composition of the present invention effectively inhibits gene expression of enzymes involved in melanin pigment metabolism synthesis, and thus has excellent skin whitening activity. Thus, the composition of the present invention has excellent skin whitening activity, As a pharmaceutical composition for treating or preventing skin pigmentation diseases such as hyperpigmentation, post-inflammatory pigmentation, and hyperpigmentation caused by dermatitis.

In the present invention, the content of the rosemary extract contained in the pharmaceutical composition is preferably 0.001 to 99.9% by weight based on the total weight of the composition, taking into account the types of skin pigmentation diseases, the severity of symptoms, ≪ / RTI >

The pharmaceutical composition may be any of the formulations that are usually applied to the skin in the art and examples thereof include creams, gels, patches, sprays, ointments, alerts, lotions, liniments, pastes, And a plasma agent.

As described above, it is used as an external preparation for skin to be absorbed into the human body, and there is no fear of side effects, and no irritation is given to the skin surface.

In addition, the pharmaceutical composition may be used in a homogeneous mixture with a pharmaceutically acceptable carrier, excipient, diluent or a mixture thereof, if necessary. Examples of such carriers, excipients or diluents include petrolatum, liquid paraffin, paraffin, A water-soluble base such as plastibase, rod, vegetable oil, wax and refined lanolin, a water-soluble base such as polyethylene glycol, an emulsion base such as an ointment ointment, a hydrophilic ointment, an antioxidant, a waterproofing agent, a moisturizer and a softening aid have.

The amount of the pharmaceutical composition to be used is not particularly limited so long as it is sufficient to completely apply the entire skin, and the number of times of application may also be applied to the skin from time to time in accordance with the convenience of the user.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Example  1. Rootten flower petal extract

After drying the petals, they were pulverized with a freeze dryer. The powder thus prepared and water were mixed at a weight ratio of 1:10, and the mixture was subjected to hot water extraction at 100 ° C for 2 hours to obtain a rose flower petal extract.

Example 2. Rawalpinia flower + stem extract

The same procedure as in Example 1 was carried out except that a rosemary extract was obtained by using a powder mixture in which rosemary flower petals and stem (branch: leaf = 1: 1 weight ratio) were mixed at a weight ratio of 1: 0.5.

COMPARATIVE EXAMPLES 1. Ruminal stalk extract

The same procedure as in Example 1 was carried out except that the stem powder mixed at a weight ratio of 1: 1 was used instead of the rosemary flower petal, and the rosemary stem extract was obtained.

Comparative Example 2 80% Ethanol

The same procedure as in Example 1 was carried out except that 80% ethanol was used instead of water as an extraction solvent to obtain a rose flower petal extract.

<Test Example>

Test Example 1. Cytotoxicity test

Cytotoxicity was measured according to the concentrations of the extracts of Walukae extract prepared in Examples and Comparative Examples.

Cytotoxicity was measured by the method of Rochem et al. In order to carry out the test in the safety concentration range of the sample. Cells (2 × 10 ⁵ cells / well) (RAW 264.7) were seeded in 24-well plates and cultured for 24 hours. After that, the medium was removed and 1 mL of the medium containing 3% of the serum in which the extract was dissolved was dispensed and cultivation was carried out for 24 hours. After 24 hours, 0.25 mL of XTT-PMS solution (1 mg XTT and 10 g PMS / mL of MEM without phenol red) was added to each well and incubated for another 2 hours. The degree of cytotoxicity was determined by measuring the absorbance of formazan at 450 nm using a microplate reader. The medium was MEM supplemented with 10% (v / v) fetal bovine serum (FBS), 0.5% (v / v) 50 g / mL streptomycin, 50 IU / mL penicillin, 0.125 g / mL fungizone, 3.024 g sodium bicarbonate And incubated at 37 ℃, 5% CO _2, 95% humidified air.

division Cell viability (%) 10 [mu] g / ml 25 μg / ml 50 μg / ml 100 [mu] g / ml Example 1 110.4 107.8 104.5 103.4 Example 2 119.5 115.6 111.1 110.9 Comparative Example 1 96.4 92.1 90.1 87.3 Comparative Example 2 94.8 90.4 80.5 61.5

As shown in the above Table 1, it was confirmed that the rosiglitazone extract prepared according to Examples 1 and 2 of the present invention showed no toxicity in the interval of 10 to 100 ug / ml, and in particular, the cell viability of the rosiglitazone extract of Example 2 The best.

On the other hand, in Comparative Examples 1 and 2, it was confirmed that the toxicity appeared in the whole section of 10 to 100 ug / ml.

Test Example  2. Melanin formation inhibitory effect

In order to confirm whether or not the extract of Walukae extract prepared in Examples and Comparative Examples reduced the production of melanin pigment, B16-F10 cells (ATCC, Manassas, USA), melanoma cells, 1 μM melanocyte stimulating hormone (MSH), a representative inducer of melanogenesis, was treated and cultured for 3 days.

The melanin content of cultured melanoma cells was measured by washing with physiological saline, dissolution with lysis buffer, and treatment with 20% TCA solution. The precipitate was washed twice with 10% TCA solution, and then sequentially treated with a mixed solution of ethanol and diethyl ether (3: 1) and diethyl ether solution, and dried in air. Then, 0.85 M potassium hydroxide Solution and heated for 15 minutes. After cooling, the absorbance was measured at 440 nm. Proteins were quantified by the Bradford method (Anal Biochem 1976; 72: 248-54) and the measured amount of melanin pigment was divided by the amount of protein to obtain 1 mg unit protein The measured amount of melanin pigment production (melanin pigment measurement amount / protein) is shown in Table 2 below. As a control, melanoma cells were treated with ugly extract only without α-MSH.

division Melamine concentration (%) α-MSH Control group albumin 10 [mu] g / ml 25 μg / ml 50 μg / ml 100 [mu] g / ml Example 1 58.8 100.1 98.4 95.1 63.3 37.5 28.9 Example 2 58.8 100.1 98.4 88.0 61.8 27.9 14.2 Comparative Example 1 58.8 100.1 98.4 100 99.4 98.2 98.5 Comparative Example 2 58.8 100.1 98.4 100 100 100 99.8

As shown in Table 2 above, it was confirmed that the extracts of Walukae extract prepared according to Examples 1 and 2 of the present invention exhibit a more excellent melanin production inhibitory effect than albumin, which is a typical melanin formation inhibitor, from 10 ug / ml or more. In particular, the writhing extract of Example 2 showed the most excellent melanin production inhibitory effect.

On the other hand, in Comparative Examples 1 and 2, it was confirmed that the melanin formation inhibitory effect was hardly exhibited in the entire section of 10 to 100 ug / ml.

Test Example  3. Tyrosinase  Activity inhibitory effect

The effects of ugly extracts on the activity of tyrosinase, which plays an important role in the formation of melanin pigment, were evaluated.

(DOPA) was oxidized to DOPA chrome by using a cell eluate of melanoma cells treated with α-MSH and urea extract of Test Example 2 by a spectrophotometer at 475 nm . The reaction was carried out by adding the cell extract to a freshly prepared substrate solution (0.1% L-DOPA in 0.1 M sodium phosphate, pH 6.0) and incubating at 37 ° C. And tyrosinase activity was corrected by tyrosinase activity per 1 mg unit protein using the protein quantification method described in Test Example 2, and is shown in Table 3 below. As a control, melanoma cells were treated with ugly extract only without α-MSH.

division Tyrosinase activity (%) α-MSH Control group albumin 10 [mu] g / ml 25 μg / ml 50 μg / ml 100 [mu] g / ml Example 1 78.5 100 79.3 77.5 69.9 63.2 43.0 Example 2 78.5 100 79.3 72.0 62.4 58.6 38.1 Comparative Example 1 78.5 100 79.3 97.8 98.9 87.2 81.5 Comparative Example 2 78.5 100 79.3 98.4 95.6 89.6 85.4

The inhibitory effect of intracellular melanin production is shown by inhibiting the activity of tyrosinase, a key enzyme of melanogenesis.

As shown in Table 3 above, it was confirmed that the extracts of Walukae extract prepared according to Examples 1 and 2 of the present invention exhibited more excellent inhibition of tyrosinase activity than albumin over 10 ug / ml.

On the other hand, it was confirmed that Comparative Examples 1 and 2 inhibited tyrosinase activity not better than albumin in the whole section of 10 to 100 ug / ml.

Test Example  4. Skin whitening effect

(Aurora UV-Light, Chaiyang Medix, Seongnam) by irradiating ultraviolet rays (UVB) of 400 mJ / cm ² onto the inside of the upper arm of a human, three times each at 2 x 2 cm ² area for 5 weeks, Deposition was induced.

The effect of the rosemary extract extract on skin whitening effect was evaluated by continuously applying the crude extract of rosemary extract to the skin of the above-mentioned pigmentation-induced human skin twice a day for 2 weeks to 10 weeks from the beginning of UV irradiation. As a control group, the other arm was irradiated with ultraviolet light under the same conditions, and the group without any treatment was used.

The skin whitening effect was measured by measuring the MI (melanogenic index) value using a mexameter (CK, Mexameter, MPA 9, Courage, Khazaka, Germany), and the difference ) Are shown in [Table 4].

division △ MI 2 weeks 4 weeks 6 weeks 8 weeks Control group 9.4 3.4 -1.2 -7.8 Example 1 -8.5 -21.5 -28.9 -32.5 Example 2 -7.0 -15.9 -22.1 -26.6 Comparative Example 1 8.9 2.9 -1.0 -8.9 Comparative Example 2 9.0 3.3 -1.0 -7.5

As shown in the above Table 4, the ura value of MIW started to decrease as soon as the extract was treated and the Δ MI value decreased steadily until 8 weeks, , The ΔMI value is linearly declined to -26 to -33, so that it acts on the skin induced by melanin pigment formation and has an excellent skin whitening effect.

On the other hand, Comparative Examples 1 and 2 were found to have no effect on whitening by showing similar values to those of the untreated control group. Melanogenesis was increased in the control group and Comparative Examples 1 and 2 by 4 weeks after irradiation with ultraviolet light, and no significant decrease of melanin production was observed even after 8 weeks.

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example  1. Manufacture of cosmetic products of emulsified formulations

Cosmetics of emulsified form such as nutrient lotion, cream, essence, and cosmetics of solubilized form such as softened longevity were prepared.

Emulsifier type cosmetics were prepared with the compositions shown in Table 5 below. The production method is as follows.

1) A mixture of raw materials 1 to 9 was heated to 65 to 70 占 폚.

2) The starting material of 10 was added to the mixture of step 1).

3) The mixture of raw materials 11 to 13 was completely dissolved by heating to 65 to 70 占 폚.

4) While the above step 3) was carried out, the mixture of step 2) was gradually added and emulsified at 8,000 rpm for 2 to 3 minutes.

5) The raw material of 14 was dissolved in a small amount of water and then added to the mixture of step 4) and further emulsified for 2 minutes.

6) The raw materials of 15 to 17 were each weighed, and then put into the mixture of step 5) and further emulsified for 30 seconds.

7) The mixture of step 6) was degassed after emulsification and cooled to 25-35 占 폚 to prepare an emulsifier-type cosmetic.

Furtherance Emulsifier type 1 Emulsifier type 2 Emulsifier type 3 One Stearic acid 0.3 0.3 0.3 2 Stearyl alcohol 0.2 0.2 0.2 3 Glyceryl monostearate 1.2 1.2 1.2 4 Wax 0.4 0.4 0.4 5 Polyoxyethylene sorbitan
Monolauric acid ester 2.2 2.2 2.2 6 Methyl paraoxybenzoate 0.1 0.1 0.1 7 P-hydroxybenzoic acid profile 0.05 0.05 0.05 8 Cetyl ethyl hexanoate 5 5 5 9 Triglyceride 2 2 2 10 Cyclomethicone 3 3 3 11 Distilled water ~ 100 ~ 100 ~ 100 12 Concentrated glycerin 5 5 5 13 Triethanolamine 0.15 0.15 0.15 14 Polyacrylic acid polymer 0.12 0.12 0.12 15 Pigment 0.0001 0.0001 0.0001 16 incense 0.10 0.10 0.10 17 Example 1: 0.0001 One 10

Formulation example  2. Solubilization  Manufacture of Cosmetics for Formulation

Cosmetics of the solubilized formulations were prepared with the compositions shown in Table 6 below. The production method is as follows.

1) 2 to 6 raw materials were put into 1 raw material (purified water) and dissolved using a mixer.

2) Raw materials 8 to 11 were completely dissolved in 7 raw materials (alcohol).

3) The mixture of step 2) was slowly solubilized by adding it to the mixture of step 1).

Furtherance Solubilization Formulation 1 Solubilization Formulation 2 Solubilization Formulation 3 One Purified water ~ 100 ~ 100 ~ 100 2 Concentrated glycerin 3 3 3 3 1,3-butylene glycol 2 2 2 4 EDTA-2Na 0.01 0.01 0.01 5 Pigment 0.0001 0.0002 0.0002 6 Example 1: 0.1 5 5 7 Alcohol (95%) 8 8 8 8 Methyl paraoxybenzoate 0.1 0.1 0.1 9 Polyoxyethylene
Hydro genide ester 0.3 0.3 0.3 10 incense 0.15 0.15 0.15 11 Cyclomethicone - - 2

Claims (10)

A cosmetic composition for skin whitening characterized by comprising an extract of Wolgye-gil as an effective ingredient, which extracts a mixture of petal and stem of Wolgye-hwa. delete [Claim 2] The skin whitening cosmetic composition according to claim 1, wherein the waxy flower extract is obtained by extracting a blend of petals and stem of a rosemary flower at a weight ratio of 1: 0.2 to 0.9. [Claim 2] The skin whitening cosmetic composition according to claim 1, wherein the rosemary extract is hydrolyzed at 80 to 120 [deg.] C for 0.5 to 3 hours. The composition according to claim 1, wherein the composition is at least one selected from the group consisting of a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritive cream, a moisturizing cream, a hand cream, Wherein the at least one cosmetic composition is at least one selected from the group consisting of a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, an emulsion, a press powder, a loose powder and an eye shadow. The skin whitening cosmetic composition according to claim 1, wherein the skin whitening is performed by inhibiting the production of melanin pigment. The skin whitening cosmetic composition according to claim 1, wherein the skin whitening is carried out by inhibiting expression of tyrosinase gene. A pharmaceutical composition for the treatment and prevention of skin pigmentation diseases, which comprises an extract of Wolgye-hwa which extracts a mixture of petal and stem of Raisinhaeae as an active ingredient. 9. The method according to claim 8, wherein the skin pigmentation disease occurs locally in the skin due to an increase in the synthesis of melanin pigment and occurs in spots, freckles, black spots, nevi, drug-induced pigmentation, &Lt; / RTI &gt; hyperpigmentation, and hyperpigmentation. [Claim 9] The pharmaceutical composition according to claim 8, wherein the wartime extract is 0.001 to 99.9% by weight based on the total weight of the composition.