KR101764729B1 - Method of manufacturing a fine dust detox drink composition and itself - Google Patents

Abstract

The present invention relates to a method for producing a fine dust detoxifying beverage composition and a composition using the method,
10% by weight of bokbunja, 21% by weight of MBA grape, 5% by weight of jujube, 4% by weight of omija, 5% by weight of oats, 5% by weight of cranberry and 30% (S10); A mixed raw material crushing step (S20) of adding a mixed raw material to a crusher and adding 0.1% by weight of vitamin C based on the total weight of the mixed raw material obtained in the raw material mixing step (S10) and crushing the mixed raw material; The decomposed raw material is introduced into an extractor and an enzymatic decomposition extraction step of adding 0.05% by weight of a cellulase and 0.025% by weight of a pectinase based on the total weight of the mixed raw materials obtained in the raw material mixing step (S10) and decomposing and extracting at 60 ° C for 1 hour (S30); An extract filtration step (S40) of filtering the extract with a filter to obtain a composition having a particle size of 60 mesh or less; (S50) in which the composition is filled in a packaging container, sealed, and sterilized at 70 DEG C for 30 minutes to obtain a final composition, thereby maximizing the pharmacological efficacy of the active ingredient contained in each raw material, It is advantageous to provide a beverage and beverage composition for oriental and non-vegitable beverages, which is absorbed into the body through fine dusts and detoxified to accumulate toxic substances and exhibits immunity and anti-inflammatory function.

Description

TECHNICAL FIELD The present invention relates to a method for producing a fine dust detoxifying beverage composition and a composition using the same,

The present invention relates to a method for producing a fine dust detoxifying beverage composition and a composition using the method. More particularly, the present invention relates to a composition for extracting active ingredients from natural herbal materials and fruit and vegetables as raw materials, Thereby preventing accumulation of harmful substances absorbed in the body due to air pollution and showing an anti-inflammatory function.

Recently, the environmental pollution problem, which is getting worse and worse, has become a serious social issue because it gradually pollutes the atmosphere and water quality, which are essential for human survival, and causes various diseases such as this.

Especially, Korea is located geographically in the Far East of the Asian continent, and the yellow dust and air pollutants pushed by westerly winds from the continent are flowing into the Korean peninsula through the western coast of Korea, which ultimately pollutes water resources and soil. It is the situation that is going crazy.

Dusts were originally called sand dust winds originating from desert widely distributed in mainland China and Mongolia. However, recently, dust particles in the air including various pollutants from China, which is undergoing rapid industrialization, It is a helpless situation because it is affecting.

Based on the annual data released by the World Health Organization, the concentration of fine dust in Seoul is estimated to be 50 ~ 70 ㎍ / M ³ , which is 2 ~ 3 times higher than the recommended standard It is 1.5 to 2 times higher than New York, LA, London and Paris, and is higher than Japan.

The sand dust contained in the dust is mainly sucked into the human body through the eyes or the nose and directly harms the human body. In addition, it affects the crops and livestock by being mixed with the rainwater during the rainfall. Especially, , It induces various respiratory diseases such as asthma and chronic obstructive pulmonary disease as well as induces various inflammation in the body parts exposed to the air such as skin and eyeball and further causes cerebral blood vessels such as stroke, Diseases, cardiovascular diseases, depression, fetal growth disorders, and the like. Therefore, urgent measures are required to be prepared.

Fine dust contains large amounts of air pollutants, especially heavy metals such as lead, mercury, cadmium, nickel, sulfurous acid gas, and nitrogen oxides, which accumulate in the body once they are absorbed and accumulate. And it is known to cause genetic diseases.

In recent years, there has been an attempt to minimize the damage caused by dust and fine dust by cleaning indoor air using an air cleaner product or wearing a mask for preventing dust and fine dust when going out. However, Is not provided.

On the other hand, the development of various foods and medicines having the function of suppressing or releasing harmful toxic substances contained in fine dusts already inhaled into the body, such as heavy metals, has been continuously carried out. For example, -0839217 and a process for producing the same are as follows.

(W / w) of lemon juice, 38 to 39% (w / w) of orange juice, 3.7 to 3.8% of sugar syrup in 12 to 15% (w / w) (w / w) of pomegranate syrup, 12.3 to 13% (w / w) of pomegranate syrup and 25 to 25.7% (w / w) of water.

Registration No. 10 - 0839217 - 0000 Published Patent No. 10-2004-0033248

The functional tea beverage for detoxifying heavy metals to which the conventional techniques as described above is applied is in the form of a beverage containing a mixture of a tea tea and a fruit juice, which is a kind of fermentation tea having pharmacological properties such as antioxidant and anti-inflammation.

It has been known that the mushroom tea has physiological activities such as antioxidation, anti-inflammation and anti-allergy through a large number of studies, but it is difficult to meet the popular taste due to its unique flavor, The preference is lower than that of green tea or tea.

In addition, although the above-mentioned prior art has mixed flavors by mixing various fruit juices in the above-mentioned prior arts, it has been found that the flavor of the tea beans can be improved by drying the tea leaves from the picking of the leaves, tearing the leaves, rubbing the tea leaves, Due to the extremely difficult manufacturing process, there is a difficulty in supplying and dispensing a comparatively expensive boa tea. When boa tea, which is commercially available in mass production, is used, the content of the effective ingredient is insignificant, It is difficult to find out.

Accordingly, the present invention has been made to solve the above-mentioned problems,

10% by weight of bokbunja, 21% by weight of MBA grape, 5% by weight of jujube, 4% by weight of omija, 5% by weight of oats, 5% by weight of cranberry and 30% (S10);

A mixed raw material crushing step (S20) of adding the mixed raw material to a crusher and adding 0.1% by weight of vitamin C based on the total weight of the mixed raw material obtained in the raw material mixing step (S10) and crushing the mixed raw material;

The pulverized raw material is put into an extractor, and 0.05% by weight of the cellulase and 0.025% by weight of pectinase are added based on the total weight of the mixed raw materials obtained in the raw material mixing step (S10), followed by enzymatic decomposition extraction Step S30;

An extract filtration step (S40) of filtering the extract with a filter to obtain a composition having a particle size of 60 mesh or less;

And a sterilizing and packaging step (S50) in which the composition is filled in a packaging container, sealed and sterilized at 70 DEG C for 30 minutes to obtain a final composition, and a composition according to the preparation method is formed It is an object of the present invention to provide a detoxifying beverage composition which can maximize the pharmacological properties of the active ingredients contained in each raw material and thereby induce the toxic substance detoxifying enzyme inhaled and accumulated in the body through yellow sand and fine dust, It is possible.

The present invention relates to a natural herbal medicine having various pharmacological properties such as prevention of respiratory diseases and detoxification of toxic substances, anti-inflammation, immunity enhancement, etc. in order to protect human body from various harmful substances contained in fine dusts inhaled through environmental respiration, An advantage of providing a health functional beverage composition having an effect of activating a body-detoxifying enzyme and inhibiting an inflammatory reaction that is involved in detoxification of harmful substances that cause various diseases by effectively extracting an effective ingredient by selecting materials and fruits and vegetables .

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing a manufacturing process according to an embodiment of a method for producing a fine dust detoxifying beverage composition of the present invention. FIG.
FIG. 2 is a flow chart showing a process for manufacturing a bellflower herb extract in an embodiment of the method for producing a fine dust detoxifying beverage composition of the present invention. FIG.
FIGS. 3 to 4 are graphs illustrating analysis results of detoxification enzyme activity according to Experimental Examples of the fine dust detoxifying beverage composition of the present invention. FIG.
5 to 7 are graphs showing the results of the anti-inflammatory activity analysis according to Experimental Example of the fine dust detoxifying beverage composition of the present invention.

Hereinafter, the method of preparing the fine dust detoxifying beverage composition and the composition of the method of the present invention will be described in more detail as follows.

FIG. 1 is a flow chart showing a manufacturing process according to an embodiment of the method for producing a fine dust detoxifying beverage composition of the present invention. FIG. 2 is a view for explaining the preparation of a fine dust detoxifying beverage composition of the present invention FIGS. 3 to 4 are graphs showing analysis results of the detoxifying enzyme activation ability according to the experimental examples of the fine dust detoxifying beverage composition of the present invention, FIGS. 5 to 7 are graphs showing the results of the experiments of the fine dust detoxifying beverage composition The results of analysis of inhibition of inflammation according to the examples will be described together as a graph.

The method for producing a fine dust detoxifying beverage composition to which the technique of the present invention is applied and the composition according to the manufacturing method can be effectively used for extracting effective ingredients with various natural herbal materials and fruits and vegetables having pharmacological properties such as detoxification, And to provide a beverage composition having remarkable functionality such as detoxification of harmful toxins and anti-inflammation by inducing the activation of the body-detoxifying enzyme and the inhibition of the inflammatory reaction.

To this end, the present invention relates to a method for producing a raw material mixture, comprising a raw material mixing step (S10) of mixing a brambly, an MBA grape, a jujube, an omija, an oodi, a cranberry and a boat and adding a bellflower herb extract to a mixing raw material; (B) adding 0.1% by weight based on the total weight of the mixed raw materials obtained in the raw material mixing step (S10), and crushing the mixed raw materials; and feeding the crushed raw materials to an extractor, (S30) in which 0.05% by weight of a cellulase and 0.025% by weight of pectinase are added based on the total weight of the mixed raw materials obtained, and the resultant is decomposed and extracted at 60 DEG C for 1 hour, and the extract is filtered using a filter, (S40) for obtaining a composition having a viscosity of 60mesh or less and a sterilization container for obtaining the final composition by filling the container in a packaging container, sealing the container at 70 DEG C for 30 minutes, It comprises the step (S50).

In the raw material mixing step (S10), a mixed raw material in a state in which each raw material is uniformly stirred at a predetermined ratio is obtained. 10% by weight of brambles, 21% by weight of MBA grapes, 5% by weight of jujubes, 4% by weight of omija, 5% by weight of odi, 5% by weight of cranberries, 30% by weight of pearls in order to maximize efficacy while minimizing destruction of effective ingredients of each raw material. Are mixed and mixed, and 20% by weight of the bellflower herb extract is added to obtain a mixed raw material.

This is according to the result of analyzing the raw materials through a plurality of pharmacological experiments and deducing the optimum combination and compounding ratio. The pharmacological properties and efficacy of each of the raw materials will be briefly described below.

Bokbunja is a fragrant, sweet and bitter fruit, rich in anthocyanin compounds and contains vitamins and various minerals. Representative pharmacological actions include anti-inflammation and antioxidant activity, and it strengthens kidney and liver function, and is effective for fever, perspiration, diuretic, detoxification, and strengthens sight and strengthens the body.

The MBA grape is Muscat Bailey A (MBA), a grape variety derived from the West, and is sometimes referred to as the grape grape in Korea. It is abundant in pulp, excellent in dewaterability, and strong in sweetness, and is known as a wine varietal. Like bokbunja, it is abundant in anthocyanin, contains a large amount of nutrients such as calcium, carbohydrates and vitamins, and is effective in antioxidation, anti-aging, and vision improvement. Resveratrol, a polyphenolic substance, Is reported to be.

Jujube has a sweet and sour taste, and the pulpy contains abundant sugar as well as citric acid and tartaric acid. The jujube seeds contain betuline, betulic acid, and fat, and are used as a medicinal product for nourishment, nervous stability, blood purification, anti-aging and diuretic in one room.

Omija has five flavors: sweet, sour, bitter, salty, and spicy. It contains citric acid, malic acid, citral, sizandrine, and high ginsin to strengthen the heart, reduce blood pressure, and strengthen the immune system. In particular, it strengthens the pulmonary function and promotes the metabolism of the liver, and is effective for respiratory diseases and hepatitis. It has no toxicity and has a beautiful color, so it is good to drink with tea or liquor.

Audi is planted with the fruit of mulberry trees and has a sour taste and a refreshing fragrance. The main ingredient contains glucose, fructose, citric acid, malic acid, tannin, pectin, vitamins, calcium, phosphorus and iron. The main pharmacological effects of diabetes, thirst, alcohol and toxin decomposition, nervous stability, hematopoiesis, insomnia, fatigue, dizziness, blood sugar and cholesterol lowering effects are effective.

Cranberry is one of the most popular fruits in the United States and Europe, with blueberries originating in North America. It is mainly consumed as juice, jam, and dry matter, and is used as a main raw material for functional health food in Korea. It is rich in anthocyanins and contains nutrients such as carbohydrates, potassium, calcium, and vitamins. Its pharmacological actions include antioxidation, anti-aging, vision improvement, cholesterol lowering, and cardio-strengthening. It is also effective in preventing bacteria from being implanted in the body and improving periodontal disease, gastric ulcer and liver function.

Pears contain more than 85% of the moisture contained in the pulp and contain carbohydrates, sugars, malic acid, tartaric acid, citric acid, and vitamins. It protects the lungs and suppresses coughing and is effective for bronchial diseases, and is effective for colds, asthma, defecation and diuretic action. In addition, anti-inflammatory and detoxifying action is effective in eliminating hangovers.

In addition to the addition of water to obtain a liquid beverage composition during the manufacturing process of the beverage composition of the present invention, the bellflower extract of the present invention can be applied to a variety of oriental herbs, fruits, vegetables and the like including bellflower having a remarkable effect on anti- Is added as a raw material to further elucidate the weakness. The concrete production process of the bellflower herb extract will be described later.

On the other hand, after mixing the raw materials having the above-mentioned weakness, the raw material crushing step (S20) is performed to a predetermined size using a crusher to facilitate the extraction process.

In the mixing raw material crushing step (S20), vitamin C is added together with the raw material before crushing in an amount of 0.1% by weight based on the total weight of the mixed raw materials obtained in the raw material mixing step (S10). Vitamin C, as well known, resists infections, heals wounds and restores tissue, especially antioxidative action to prevent damage to the cell, such as adding, so that the nutrients contained in the raw materials to balance .

In the enzymatic decomposition extraction step (S30), an extract is obtained through an enzymatic hydrolysis extraction method. That is, by adding a pectinase and a cellulase enzyme to the crushed mixed raw materials, the effective ingredients of the raw materials are effectively extracted.

The cellulase is an enzyme that hydrolyzes cellulose to produce cellobiose. Pectinase is an enzyme that hydrolyzes pectin, which is a carbohydrate polymer, and decomposes and softens cell walls and fibrils of plant cells.

Generally, herbivores produce cellulase in the body to digest enough fiber. However, since human can not produce cellulase directly in the body, the fiber can not be digested and is discharged or partially decomposed by E. coli.

Accordingly, in the enzymatic decomposition and extraction step (S30), it is necessary to effectively soften the fibers contained in the raw materials used in the present invention and to decompose and extract the solid and liquid extracts, 0.05% by weight of cellulase and 0.025% by weight of pectinase.

In addition, extracts are extracted for 1 hour at a relatively low temperature of 60 ° C to facilitate digestion and absorption during drinking, and inhibit the formation of insoluble sediments, thereby softening the texture.

In the extract filtration step (S40), the solid material contained in the extract extracted in the enzymatic decomposition extraction step (S30) is filtered and filtered using a 60 mesh filtrate to obtain a liquid having a concentration suitable for drinking.

In the sterilization and packaging step (S50), various harmful bacteria remaining after the preparation process of the composition are removed before the packaging of the composition, but the paste is pasteurized at 70 DEG C for 30 minutes to minimize the destruction of nutritional components.

Meanwhile, the process for manufacturing the first step of the first step of the first step of the raw material mixing step (S10) is as follows.

(S100) in which water is mixed with water at a weight ratio of 3:10 and extracted for 2 hours, and a step (S100) of extracting water from the raw water, which is a mixture of ginger, bellflower, grape seed, hwanggi, licorice, cinnamon, Chinese cabbage, A first cooling step (S200) of cooling the extract obtained at the first hot water extraction step (S100) at room temperature for 20 hours, and cooling the solid material using the filter material to obtain a powder having a particle size of 60 mesh or less A first secondary filtration step S300 for obtaining a primary extract and a second secondary hot water extraction step S400 for collecting the solid filtered in the primary filtration step S300, A second cooling step (S500) of cooling the extract obtained at the second hot water extraction step (S400) at room temperature for 20 hours; cooling the cooled extract using a filter to obtain 60mesh (S600) for obtaining a second extraction liquid having a concentration A mixture of a second tea extract obtained in the first extract and the second filtering step (S600) obtained in the filtering step (S300) comprises a mixing completion (S700) to complete the final bellflower herbal extract.

In the first primary hot water extraction step (S100), the raw materials are mixed at a predetermined ratio into an extraction vessel, followed by hot water extraction for 2 hours to obtain an extract. 20% by weight of ginger, 40% by weight of bellflower, 1% by weight of grape seed, 3% by weight of grape seed, 10% by weight of grape seed, 2% by weight of licorice, 20% by weight of cinnamon, 1% by weight of cabbage, 5% by weight of bank, and 8% by weight of jujube are mixed with water at a weight ratio of 3:10 and extracted.

This is in accordance with the results obtained by analyzing the raw materials through a plurality of pharmacological experiments and deducing optimum combination and blending ratio. The pharmacological characteristics and efficacy of each raw material including the bellflower are as follows.

Ginger has more than 85% moisture and contains a large amount of minerals. It has a unique flavor due to its gingerol ingredient, it has a powerful sterilizing action against various pathogenic bacteria and acts as a pharmacological effect on heart strengthening, blood circulation promotion, chills, fever, headache and sputum. Diastase and proteolytic enzymes are also effective in promoting digestion and intestinal motility.

Platycodon is rich in carbohydrates, calcium, iron and fiber, and especially contains saponin and fliticone, which are the main components of ginseng, and acts as a pharmacological agent for antiinflammation, germane, antiulcer, shinhae, fever and analgesia. It is also used as a medicinal product because it has an effect of lowering blood sugar and cholesterol.

Grape seed is rich in antioxidant ingredients such as catechin and polyphenol to prevent aging, anti-inflammatory, anti-allergic and anti-viral functions to strengthen the immune system and lower cholesterol, which is also effective in vascular disease.

Hwanggi uses roots as medicines and contains polyacids, cholines, etc., and acts on the central nervous system as excitatory, tonic, and diuretic, and has a pharmacological action to inhibit nephritis, proteinuria, cholesterolemia, and lower blood pressure. It has also been used as a substitute for ginseng since it has efficacy for body weakness, fatigue bloating, spasmolithiasis, cold sweat, peripheral nerve palsy, edema, chronic nephritis.

Licorice is a medicinal herb that is used all over the world because it harmonizes the toxicity of all drugs and makes them appear more effective. It has the effect of strengthening blood circulation and musculoskeletal by regulating the intestines and fraud. It contains glycyrrhizin, glabric acid, sucrose, glucose, liquiritin, lycoricidine, etc. Glycyrrhizin has the effect of detoxifying diphtheria toxin, snake venom and blowfish poison. The main pharmacological action is detoxification, hepatitis, dermatitis, Jinhae, geomorphic, muscle relaxation, diuretic, anti-inflammation, acts and inhibits gastric and duodenal ulcers. Glycyrrhizin, a major component of licorice, inhibits enzymes metabolizing steroids, resulting in abnormal hormonal regulation when taken in large doses.

Cinnamon is rich in carbohydrates, minerals, and vitamins, warms the inside, keeps the blood vessels well, evenens the lungs and liver, and activates the nonfunctioning function. Therefore, it has effects on nourishment, sweating, fever, analgesia and fragility, and has a unique flavor.

Chinese cabbage is mainly used for soaking kimchi in Korea, and most of the water is rich in carbohydrates, vitamins, carotene, minerals such as calcium and phosphorus. Therefore, it is effective in fever, thirst, and digestion. Especially, it is known that the cinchinol component contained in the cabbage leaves is involved in the anticancer action.

The bank is called "白果 (白果)" in the oriental medicine, and it is used as a nourishing agent by acting as a ginger. It contains Jangsoo Platonic component to improve blood circulation, so it can prevent various vascular diseases, has an effect to regain energy, and is effective in preventing aging. Since it contains a toxic substance called 4-methoxypyridoxine (MPN), it is desirable not to consume more than the recommended amount.

The pharmacological action of jujube is omitted because it is similar to the above description.

Meanwhile, the extract extracted in the first hot water extraction step (S100) is naturally cooled at a room temperature through a first cooling step (S200).

The cooled extract is filtered through a first filtering step (S300) and filtered using a 60 mesh filtrate to form a liquid phase having a proper concentration, thereby obtaining a first extract.

The step of extracting the second hot water (S400) is a step of extracting the filtered solids again in the first filtering step (S300) so that the remaining effective ingredient can be extracted sufficiently.

The extract extracted in the second hot water extraction step (S400) is naturally cooled at a room temperature through a second cooling step (S500), and the remaining solid through a secondary filtration step (S600) is filtered using a 60 mesh filtrate Followed by filtration to obtain a second extract.

Finally, the first tea extract obtained in the first tea filtration step (S300) and the second tea extract obtained in the second tea filtration step (S600) are mixed through the mixing completion step (S700) And is used together with the other raw materials in the above-described raw material mixing step (S10).

As described above, there is an advantage that the extraction efficiency can be maximized while minimizing the destruction of the effective nutrients contained in the raw material by repeating the extraction process of the Drosophila orientalis extract twice.

Hereinafter, embodiments and experimental examples including the present invention having the above-described configuration will be described, and the effect of the embodiments will be grasped thoroughly.

≪ Example 1 >

Preparation of the extract of the herbal medicine of the present invention.

Ginger 60g, bellflower 120g, grape seed 3g, Hwanggi 9g, licorice 6g, cinnamon 60g, cabbage 3g, bank (bagasse) 15g, jujube 24g are prepared and mixed with 1ℓ of water and water is extracted for 2 hours.

The extract was cooled at room temperature for 20 hours and then filtered through a 60 mesh filtrate to separate the solid and obtain a first extract.

The solids were collected, mixed with 1 liter of water, mixed and extracted with hot water for 2 hours, cooled at room temperature for 20 hours, filtered through a 60 mesh filtration sieve, and the second extract was obtained.

The first tea extract and the second tea extract are mixed to complete an extract of Bellflower Herb Extract.

≪ Example 2 >

Preparation of the fine dust detoxifying beverage composition of the present invention.

200 g of bokbunjae, 420 g of MBA grape, 100 g of jujube, 80 g of omija, 100 g of oats, 100 g of cranberry and 600 g of pears were mixed and 400 g of the herb extract obtained in Example 1 was added to obtain a mixed raw material.

The mixed raw material is introduced into a crusher, and 0.1% by weight based on the total weight of the mixed raw materials obtained in the raw material mixing step (S10) is added to crush the vitamin C.

The crushed raw materials are added to the extractor, and the hydrolytic enzymes, such as cellulase and pectinase, are added in amounts of 0.05 wt% and 0.025 wt%, respectively, based on the total weight of the mixed raw materials obtained in the raw material mixing step (S10). Followed by decomposition and extraction at 60 ° C for 1 hour.

The extract was filtered through a 60 mesh filter to obtain a composition. The composition was sealed in a packaging container and sterilized at 70 ° C for 30 minutes to complete a fine dust detoxifying beverage composition.

Table 1 below shows the analysis of the active ingredients contained in the finished fine dust detoxifying beverage composition.

Raw material Content (% by weight) Active ingredient Bokbunja 10 sanguinin H-4, gallic acid, quercetin, ellagic acid and various phenolic acids MBA Grape 21 Vincifer, amurensin, heyncanol Jujube 5 Juubosides A1 and C, aceryljujuboside B, spinosine, magnofluorine Schisandra 4 Inulin, lignin (arctigenin, arctin), terpenoids Audi 5 Anthocyanin Cranberry 5 Anthocyanin, Proanthocyanidin ship 30 Polyphenol compound Bellflower herbal extract 20 Platycodigenin I, platycodigenic acid A, B, C, botulin, a-spinasterol

Hereinafter, Experimental Examples 1 to 2 for confirming detoxification enzyme activity and inflammation inhibiting ability of the fine dust detoxifying beverage composition of the present invention will be described.

The detoxifying enzyme activation ability is a detoxifying enzymatic action mechanism mainly occurring in the liver responsible for the detoxification of the body. It activates the phosphorylation enzyme including the mitogen-activated protein kinase (MAPK) in the cell due to the stimulation of the chemical component from the outside, Nrf2, which is a transcription factor, migrates into the nucleus and binds to the promoter gene, thereby detoxifying the toxic substance through the action of the expression and activation of the detoxifying enzyme protein.

Inhibition of inflammation is caused by activation of inflammatory signal pathway caused by external inflammatory factors, among which NFkappaB, which is a transcription factor, is transferred into the nucleus and binds to NFkappaB site to secrete inflammatory cytokine Therefore, NFkappaB signaling to inhibit the secretion of inflammatory cytokines or inhibit the secretion of selected ingredients to induce inflammation is induced.

<Experimental Example 1>

Analysis of Detoxification Enzyme Activation Ability of the Fine Dust Detox Beverage Composition of the Present Invention.

To confirm the detoxification effect, the activation of the two-phase detoxifying enzyme system was confirmed using the colorimetric method. Hepa1c1c7 cells, hepatocellular carcinoma cells of the experimental animals, were treated with the raw materials of the micro-dust detoxifying beverage composition of the present invention prepared in Examples 1 and 2, and NQO1 activation, a two-phase detoxifying enzyme indicator enzyme, was measured. As shown in the graphs shown in Figs. 3 to 4.

As a result, it was confirmed that tBHQ was used as a positive control and NQO1 enzyme activity, which is similar to or higher than that of the positive control group, was found in the raw material of beverage detergent composition of the present invention, and grape seed, The bloom extract showed a similar NQO1 enzyme activity, though slightly lower than the positive control group. In addition, NQO1 enzyme activity was lower in blueberry, raspberry, audi and garlic than in the positive control group, but the enzyme activity was stronger than the control group.

These results suggest that quercetin, terpenoids, anthocyanin, and the like contained in the raw material added to the fine dust detoxifying beverage composition of the present invention induce a two-phase detoxifying enzyme system, Was able to verify the enzymatic activity of the detoxification enzyme.

<Experimental Example 2>

Analysis of inflammation inhibition ability of the fine dust detoxifying beverage composition of the present invention.

(1) In order to confirm the inhibition of inflammation, lung cancer cells are used to evaluate the efficacy of IL-6 (interleukin 6), an inflammatory cytokine, in respiratory diseases.

First, lung cancer cells were cultured and IL-6 was identified using an conditioned medium after treating the fine dust detoxifying beverage composition of the present invention prepared in Examples 1 and 2, 5 as shown in the graph.

As a result, it was confirmed that the fine dust-detoxifying beverage composition slightly reduced IL-6 by treatment with LPS, which is an exogenous inflammation inducer.

(2) In order to confirm the change in the amount of inflammatory cytokine secretion in the microenvironment through co-culture of macrophages and lung cancer cells, which initiate the inflammatory reaction, THP-1 monocytes ) Was treated with TPA to differentiate into macrophages. Pretreatment of the micro-dust detoxifying beverage composition of the present invention into lung cancer cells, followed by co-culturing of the two cells, IL-6 secretion was assayed in the culture medium after treatment with LPS, And the results are as shown in the graph shown in FIG.

As a result, IL-6 was increased by 200 pg / ml when LPS was treated with the inflammation inducer, and IL-6 was remarkably decreased by pretreatment of the fine dust detoxifying beverage composition. Therefore, it was confirmed that the detoxifying beverage composition of the present invention alleviated lung inflammation.

(3) The efficacy evaluation test of the fine dust detoxifying beverage composition of the present invention was carried out in an experimental animal treated with benzoprene (benzo [a] byrene), which is a strong fine dust component.

Benzopyrene is a strong carcinogen with five benzene rings. It is absorbed into the lungs through the bronchial mucous layer after inhalation and irritates the skin. It is also transformed into the toxic metabolite, benzopyran 7,8-diol-9,10 epoxide, by the cytochrome P450 1A1,1A2,1B2, which is a 1-phase enzyme in the body.

The more specific toxic action and the mechanism to decode it are outlined below.

The benzopyran is bound to an aryl hydrocarbon receptor and the arylhydrocarbon is transferred into the nucleus by an arylhydrocarbon nuclear translocator and activates the cytochrome P450 promoting gene to activate the CYP450 protein and activate the enzyme Benzopyran is transformed into a more potent toxic component, causing oxidative stress and inflammatory responses.

Therefore, the experiment is conducted to activate the Nrf2 / 2 phase detoxifying enzyme action mechanism by the detoxification mechanism which can inhibit it.

Over the course of a total of 15 days, the fine dust detoxifying beverage composition of the present invention was orally administered to experimental animals at intervals of two days. On the 16th day, 1 mg of benzopyran was administered to each animal, sacrificed the next day to collect blood and tissues, and the blood was separated into plasma, and the secretion amount of IL-6, an inflammatory cytokine, was analyzed. As shown.

As a result, the amount of secreted IL-6, TNF alpha and IL-1beta in blood was increased in the group treated with benzopyran, and when the composition of the present invention was administered by concentration, It was confirmed that the secretion amount of cain was reduced.

Through the above-described Experimental Examples 1 to 2, it was verified that the fine dust-detoxifying beverage composition of the present invention exhibits detoxification enzyme activity and inflammation inhibitory activity in vivo and in vivo.

The method for producing a fine dust detoxifying beverage composition of the present invention having the above-described composition and the composition for the manufacturing method according to the present invention can be applied to a natural herbal medicine having a function capable of protecting the human body from various harmful substances generated by air pollution And the effective ingredient is effectively extracted by selecting materials and fruits and vegetables to prepare a fine dust-detoxifying beverage composition.

Accordingly, the present invention provides a health functional beverage composition having an excellent effect of detoxifying poisonous substances that are absorbed and accumulated in the body due to inhalation of yellow dust or fine dusts, causing various diseases, and preventing anti-inflammatory and respiratory diseases, and enhancing immunity There are various advantages of.

S10: raw material mixing step
S20: Mixing raw material crushing step
S30: Enzymatic decomposition extraction step
S40: Extraction filtration step
S50: Sterilization packaging step
S100: First hot water extraction step
S200: First cooling step
S300: First filtration step
S400: Second hot water extraction step
S500: Second cooling step
S600: Second filtration step
S700: Mixed completion stage

Claims (3)

10% by weight of bokbunja, 21% by weight of MBA grape, 5% by weight of jujube, 4% by weight of omija, 5% by weight of oats, 5% by weight of cranberry and 30% (S10);
A mixed raw material crushing step (S20) of adding the mixed raw material to a crusher and adding 0.1% by weight of vitamin C based on the total weight of the mixed raw material obtained in the raw material mixing step (S10) and crushing the mixed raw material;
The pulverized raw material is put into an extractor, and 0.05% by weight of the cellulase and 0.025% by weight of pectinase are added based on the total weight of the mixed raw materials obtained in the raw material mixing step (S10), followed by enzymatic decomposition extraction Step S30;
An extract filtration step (S40) of filtering the extract with a filter to obtain a composition having a particle size of 60 mesh or less;
And sterilizing and packaging the composition (S50) in which the composition is filled in a packaging container, sealed, and sterilized at 70 DEG C for 30 minutes to obtain a final composition. The method according to claim 1,
The bellflower herbal extract solution may contain,
A raw material mixed with 20% by weight of ginger, 40% by weight of bellflower, 1% by weight of grape seed, 3% by weight of sulfur, 2% by weight of licorice, 20% by weight of cinnamon, 1% by weight of cabbage, 5% by weight of bank, A first hot water extraction step (S100) in which water is mixed at a weight ratio of 3:10 and extracted for 2 hours;
A first cooling step (S200) of allowing the extract obtained in the first hot water extraction step (S100) to be left at room temperature for 20 hours to cool;
A first primary filtration step (S300) of filtering the solid material by using the filtered extract to obtain a first extract having a particle size of 60 mesh or less;
A second hot water extraction step (S400) of collecting the solid filtered in the first filtering step (S300), mixing the resultant with 1 liter of water and extracting it for 2 hours;
A second cooling step (S500) of allowing the extract obtained in the second hot water extraction step (S400) to be left at room temperature for 20 hours to cool;
A second filtration step (S600) of filtering the cooled extract using a filter to obtain a second extract having a particle size of 60mesh or less;
And a mixing and finishing step (S700) of mixing the first extraction liquid obtained in the first filtration step (S300) and the second extraction liquid obtained in the second differential filtration step (S600) to complete the final Dorage herbal extract &Lt; RTI ID = 0.0 &gt; 1, &lt; / RTI &gt; 10% by weight of brambles, 21% by weight of MBA grapes, 5% by weight of jujubes, 4% by weight of omija, 5% by weight of oats, 5% by weight of cranberries, 30% by weight of pears and 20%
The bellflower extract solution contained 20% by weight of ginger, 40% by weight of bellflower, 1% by weight of grape seed, 3% by weight of sulfur, 2% by weight of licorice, 20% by weight of cinnamon, 1% Wherein the detergent composition is a detergent composition.

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