KR101695299B1 - Composition for preventing or treating obesity or hyperlipidemia containing Piper longum extract, soy extract containing isoflavon and L-carnitin - Google Patents

Abstract

The present invention relates to a composition for preventing or ameliorating obesity, and more particularly, to a composition for preventing or improving obesity, which comprises body lotion and body weight by simultaneously containing a petal extract, a soybean extract containing isoflavone and L-carnitine as an active ingredient And to a composition for preventing or improving obesity that provides a heat production promoting effect that increases energy consumption without increasing the amount of activity.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for prevention or treatment of obesity or hyperlipidemia containing soybean extract containing L-carnitine, a petroleum-derived extract, a soybean extract containing isoflavone,

The present invention relates to a composition for preventing or ameliorating obesity or hyperlipidemia. More particularly, the present invention relates to a composition for preventing or ameliorating obesity or hyperlipidemia, To a composition for the prevention or improvement of obesity or hyperlipidemia which provides a thermogenesis promoting effect of reducing energy consumption and increasing energy consumption without increasing activity.

Because weight changes occur by the difference in total energy intake and consumption, you need to reduce your energy intake or increase energy consumption to lose weight. The energy consumption of the human body can be divided into several components including the main components of rest and activity. Total daily energy expenditure (TEE) is composed of resting metabolic rate (RMR), thermic effect of food (TEF) and energy expenditure from physical activity .

"Heat production of food" refers to energy consumption that is higher than the basal metabolic rate (BMR) due to food intake, cold exposure, thermogenic agents, and psychological effects, That is, the energy can be consumed without movement. The organelles responsible for this heat generation in the body are mitochondria, where nutrients are produced as uncontrolled oxidative phosphorylation (ATP) and as heat. UCP (uncoupling protein) is a protein involved in this heat-producing reaction. When inducing UCP expression or increasing activity, heat production is increased and body weight is decreased (Nedergaard J., et al., EMBO Rep. 917-921, 2005). In addition, the increase in body weight in most obese patients after stopping the low calorie diet can be explained as a thermogenic defect. Heat generation defects can save up to 125 kcal of energy per day. An increase in energy consumption of less than 25 kcal / day for an increased body weight (kg) can account for a weight gain of 5 kg due to the effect of heat generation defects.

Recently, inhibitors of CB1 (cannabioid type I) receptors have been reported to promote the conversion of white fats to brown fats and increase heat production (Perwitz N., et al., Diabetes, Obesity and Metabolism, 12: 158 -166, 2010). However, since people avoid cold exposure through clothing or room temperature control, the effect of energy consumption through promotion of heat generation mechanism alone in everyday life is not significant.

On the other hand, hyperlipidemia is a state in which many fat components are present in the blood more than necessary and accumulate in the blood vessel walls to cause inflammation, resulting in cardiovascular diseases. Recently, abnormal blood lipid status is defined as dyslipidemia. Genetic factors increase the specific lipid in the blood and often cause hyperlipemia, but other causes such as obesity, alcohol, and diabetes can also cause hyperlipemia.

Accordingly, the inventors of the present invention found that simultaneous treatment of L-carnitine with soybean extract containing L. extract, Isoflavone, and L-carnitine provides a promoting effect of thermogenesis which decreases body fat and body weight and increases energy consumption without increasing activity Thereby completing the present invention.

Accordingly, it is an object of the present invention to provide a composition for preventing or improving obesity that can feel weight loss and heat generation as an immediate cognitive efficacy.

It is also an object of the present invention to provide a composition for treating or preventing hyperlipidemia which lowers the content of triglyceride or cholesterol in blood.

In order to achieve the above object, the present invention provides a composition for preventing or improving obesity, which comprises a petal extract, a soybean extract containing isoflavone, and L-carnitine as an active ingredient.

In addition, the present invention provides a composition for treating or preventing hyperlipidemia, which comprises a petal extract, a soybean extract containing isoflavone, and L-carnitine as an active ingredient.

The composition according to the present invention promotes thermogenesis, which includes a petal extract, a soybean extract containing isoflavone, and L-carnitine as active ingredients, thereby decreasing body fat and body weight and increasing energy consumption without increasing activity Obesity and the like. The composition according to the present invention was also effective in treating or preventing hyperlipidemia by significantly reducing the content of triglyceride and cholesterol in blood.

Accordingly, the composition according to the present invention can be used in various fields such as foods and medicines for preventing or treating obesity and hyperlipemia.

1 and 2 are graphs showing weight loss effects of test substances in male C57BL / 6 mice in which obesity is induced by high fat diet.
FIG. 3 is a graph showing the effect of test substances on epididymal fat weight loss in male C57BL / 6 mice induced by obesity by high fat diet.
FIG. 4 is a graph showing the effect of enhancing the expression of thermogenesis-related protein (UCP1) in male C57BL / 6 mice induced by obesity by high fat diet.

The present invention relates to a composition for preventing or improving obesity, which comprises a petal extract, a soybean extract containing isoflavone, and L-carnitine as an active ingredient. The synergistic effect of each of the above- A composition for prevention and improvement of obesity showing body fat and weight loss effect is provided. In addition, the composition according to the present invention can treat or prevent hyperlipidemia by lowering the content of cholesterol and triglyceride in blood.

: Piper longum L.)은 필발(

)에서 유래되었으며, 생김새는 원주형으로 과축(果軸)의 주위에는 작은 알맹이의 열매가 무수히 붙어 있어 그물눈 모양을 나타내고, 바깥면은 적갈색이나 흑갈색이다. 열매의 위쪽에 셋으로 갈라진 주두(柱頭)가 있고 아래쪽은 과축이 붙어 있다.The

: Piper longum L.)

), And its appearance is cylindrical. Around the peripapillary axis, there are a lot of fruit of a small grain, and the outer surface is reddish brown or dark brown. On top of the fruit is a three-pronged headstock and the bottom is attached with an axilla.

The petal extract used in the present invention can be prepared by subjecting the petals to hot water extraction based on a conventional herbal medicine extraction method, followed by separation and purification. In one embodiment of the present invention, distilled water is pulverized 10 times Extracting it from a hot water tank for about 3 hours; And filtrating the filtrate obtained in the extraction step, followed by lyophilization and evaporation and concentration, but the present invention is not limited thereto. In the present invention, it was confirmed that the present invention promotes the expression of UCP1, which is a heat-production-related protein, thereby promoting heat generation, which increases energy consumption without increasing activity.

The composition according to the present invention may contain 0.0001 to 10% by weight based on the total weight of the composition, and if the content of the extract is less than 0.0001% by weight, the effect of promoting heat generation is insignificant, The formulation stability may be reduced.

Isoflavones used in the present invention have been reported to exhibit diverse physiological activities as a female hormone-like vegetable hormone, which is a component rich in soybeans. In recent years, there have been various studies such as the regulation of fat metabolism and the reduction of blood cholesterol Results have been reported. In the present invention, the isoflavone itself may be used, or soybean extract containing isoflavone may be used. The soybean extract may be commercially available or may be prepared by an extraction method commonly known in the art.

The composition according to the present invention may contain the isoflavone in an amount of 0.0001 to 10% by weight based on the total weight of the composition, and in the case of using the soybean extract containing isoflavone, the isoflavone may be added in an amount of 0.001 to 30% Of soybean extract. If the content of the isoflavone or soybean extract is less than the proper range, the fat and weight loss effect is insignificant. If the content exceeds the proper range, the formulation stability may be decreased.

L-Carnitine used in the present invention is known to be an important component for generating energy by oxidizing fat as a component that is synthesized in liver and kidney of a normal person and contained in a lot of red meat. Deficiency of L-carnitine reduces the concentration of fatty acids in the mitochondria, thereby reducing energy production. In addition, CPT-1, which uses L-carnitine as a substrate, acts as a rate-limiting enzyme for fatty acid oxidation among various enzymes involved in fatty acid oxidation (Eaton, Prog Lipid Res 41 (3): 197-269, 2002).

The composition according to the present invention may contain 0.001 to 40% by weight of L-carnitine based on the total weight of the composition. When the content is less than 0.001% by weight, the body fat and weight loss effect is insignificant, The formulation stability may be reduced.

The setting of the content range of the active ingredients may be synergistic to the efficacy of the human body when it is commercialized, and may be set to reflect the characteristics of each ingredient in terms of safety. In addition, the range of the maximum weight was limited in consideration of the molding conditions of the active ingredient suitable for the formulation of the product.

The composition according to the present invention can be applied in the form of oral medicines and foods and has an effect of promoting energy consumption by increasing the expression of UCP1, which is a major biomarker involved in the heat production mechanism while lowering body fat . This is because the composition of the present invention simultaneously contains the soybean extract containing L-carnitine and L-carnitine and the L-carnitine extract, thereby promoting lipid degradation, fat burning and heat generation mechanisms through different mechanisms in the adipocytes, , Which provided more weight loss effects compared to soybean extract containing isoflavones and L-carnitine alone. In addition, by significantly reducing the content of triglyceride and cholesterol in the blood, the treatment or prevention of hyperlipidemia was provided.

The composition of the present invention may be prepared by appropriately selecting ingredients commonly used in the art besides the above-mentioned ingredients, and thereafter tableting the composition in the form of tablet, capsule, soft capsule, pill, granule, drink, diet bar, chocolate, caramel, It can be used as a health food, medicine, and the like.

Hereinafter, the present invention will be described more specifically with reference to examples and test examples. It is to be understood that the scope of the present invention is not limited to these embodiments, and variations, substitutions, and insertions conventionally known in the art may be carried out, And this is included in the scope of the present invention.

[Preparation Example 1] Preparation of a petal extract

In the present invention, Chinese-made petals were used. After pulverizing the petals, distilled water was added 10 times as much as the content of the petals, and the filtrate was extracted for 3 hours in a hot water bath. The resulting filtrate was filtered, freeze-dried and evaporated to obtain a petal extract.

[Example 1]

The mixture of Example 1 was prepared by mixing the petal extract prepared in Preparation Example 1, soybean extract (isoflavone content: 10.76 wt%) and L-carnitine in a weight ratio of 1: 0.75: 1.

[Test Example 1] Weight loss effect in male C57BL / 6 mice

To conduct this experiment, 10 mice per 7 weeks old C57BL / 6J male rats were prepared. After 1 week of adaptation period, the mice were separated from each other and cultured at 12 hour intervals (7 to 17 hours) . 3) "High-fat soybean extract + isoflavone-containing soy extract + L-carnitine (350 mpk)", 4) "High fat diet" Carnitine (350mpk) + soybean extract containing isoflavones + L-carnitine (350mpk) + Lobster extract (200mpk) ", 5) (200mpk) group, and 6) "high fat diet + 200ml / kg group extract" group, and they were orally administered once a day for 8 weeks at a certain time (10:00 am) , And 10 animals of the control group, high-fat diet group, were given the same amount of water. It has been widely known that tea catechins contain more than 80% catechins and have an anti-obesity effect by reducing body fat (Thielecke F., et al ., Phytochemistry, 70: 11-24, 2009). The tea catechin was added to a mixture of soybean extract containing isoflavone and L-carnitine to verify synergistic efficacy. At this time, as shown in Table 1, the total calories of the high-fat diet and normal diet are 4,057 kcal, while the high fat diet accounts for 60% of the total calories and the normal diet accounts for 10% of the fat.

ingredient (1) (g / kg) (2) (g / kg) Casein 200 200 Corn powder 0 315 Maltodextrin 125 35 Sucrose 68.8 350 cellulose 50 50 Soybean oil 25 25 Lard 245 20 Mineral Mix 10 10 Calcium phosphate 13 13 Calcium carbonate 5.5 5.5 Potassium citrate 16.5 16.5 Vitamin mix (see Table 8) 10 10

1) syntheric diet D12494 (Research Diets, Inc)

2) syntheric diet D12450B (Research Diets, Inc)

Body weights were measured once a week (11 am) during the experimental period. After 8 weeks from the start of administration, the body weight change was calculated by measuring the final body weight of the experimental group and the control group. The results are shown in Table 2, Respectively.

Test substance Weight before start of experiment (g) Weight after experiment (g) Weight change (%) Normal diet (n = 10) 18.78 ± 0.82 26.3 ± 1.78 40.4% High-altitude mode (n = 10) 20.28 ± 0.62 34.1 ± 2.16 68.5% High fat diet + soybean extract containing isoflavones + L-carnitine (n = 10) 20.63 + - 1.05 34.0 ± 3.3 64.9% High fat diet + soybean extract containing isoflavone + L-carnitine + petal extract (n = 10) 19.03 + - 0.54 27.1 + 1.63 42.5% * High fat diet + soybean extract containing isoflavones + L-carnitine + tea catechin (n = 10) 20.23 + - 0.85 34.4 ± 3.41 69.7% High fat diet + Lilium extract (n = 10) 19.98 ± 0.87 33.9 ± 2.45 69.7%

* p < 0.05

FIG. 1 shows a process of changing the body weight according to time in each population group, and FIG. 2 shows an increase in body weight. In the results of Table 2, Figs. 1 and 2, "soybean extract containing + isoflavone + L-carnitine intake group in high fat diet", "soybean extract containing high fat + isoflavone + L-carnitine + Ingestion group "and" high fat diet + fat extract group "did not show a specific weight loss effect as compared with the control group fed only with high fat diet. On the other hand, it was confirmed that the weight change was about 42.5% after 8 weeks in the case of "soybean extract containing high fat diet + soy isoflavone + L-carnitine + petal extract" &Lt; 0.05).

Therefore, the combination of the soybean extract + L-carnitine and the L. carnitine extract containing isoflavones according to the present invention is superior to the combination of the soybean extract / L-carnitine containing isoflavones statistically to suppress the weight gain Respectively. However, it was confirmed that only L. extract alone had no effect of inhibiting weight gain. Dietary intake was not significantly different among the experimental groups.

[Test Example 2] Evaluation of adipose tissue in male C57BL / 6 mice

In order to investigate the effect of the composition of the present invention on the fat reduction of the obese animals induced by the high fat diet, the fat diet of the high fat diet was divided into two groups, namely, "normal diet", "high fat diet (control group)", L-carnitine + L-carnitine extract group "," High fat diet + soybean extract containing isoflavone + L-carnitine supplement group "," High fat diet group + soybean extract containing isoflavone + L-carnitine + tea catechin ingestion group "and" high fat diet group + petal extract ingestion group "for 8 weeks. At the end of the diet, liver and epididymal fat were sacrificed. The extracted tissues were washed with physiological saline, lightly removed from the filter paper, and weighed. The results are shown in Table 3 and FIG. 3, respectively.

Test substance Liver (g / body weight g) Epididymis
(g / body weight g) Normal diet (n = 10) 47.15 + 2.43 24.39 + - 5.36 High-altitude mode (n = 10) 31.1 ± 1.99 ^a 62.6 ± 9.65 ^a High fat diet + soybean extract containing isoflavones + L-carnitine (n = 10) 31.7 ± 2.83 54.8 ± 14.84 High fat diet + soybean extract containing isoflavone + L-carnitine + petal extract (n = 10) 32.3 ± 1.83 39.8 ± 5.53 ^b High fat diet + soybean extract containing isoflavones + L-carnitine + tea catechin (n = 10) 31.8 ± 2.56 61.1 ± 11.96 High fat diet + Lilium extract (n = 10) 31.9 ± 4.56 63.3 ± 9.56

^a p <0.05 vs normal, ^b p <0.05

In the results of the above Table 3, the weight of the epididymal adipose tissue of each population was calculated to be 62.6 ± 9.65 g for "high fat diet fat", "soy fat fat fat containing soy isoflavone + high fat fat fat" , And "high fat diet + dietary supplement intake" was 54.8 ± 14.84 g, 61.1 ± 11.96 g for the soybean extract + high carotene + tea catechin intake group with high fat diet + isoflavone, and 63.3 ± 9.56, but it was 39.8 ± 5.53 g in the case of "high fat diet + soybean extract containing isoflavone + L-carnitine + fillet composite intake group".

In addition, in the "soybean extract + soybean extract containing isoflavone + soybean extract + L-carnitine + petal extract extract group", "soybean extract + soybean extract containing isoflavone + L- carnitine group" Carnitine + tea catechin supplemented with soybean extract containing isoflavone + L-carnitine + tea catechin ", the weight of epididymal adipose tissue was significantly reduced. Thus, it can be seen that the combined extract of soybean extract, L-carnitine and petal extract containing isoflavone according to the present invention has a synergistic effect on body fat reduction.

[Test Example 3] Evaluation of blood biochemical component efficacy in male C57BL / 6 mice

In order to investigate the effect of the composition of the present invention on the serum lipid metabolism-related indicators of obesity animals induced by high-fat diet, "normal diet", "high fat diet (control group)", "high fat diet L-carnitine supplemented soybean extract + L-carnitine supplemented soybean extract + L-carnitine supplemented soybean extract + L-carnitine soybean extract containing isoflavone + high- Soybean extract + L-carnitine + tea catechin ingestion group "and" high fat diet group + petal extract ingestion group "for 8 weeks. After the experiment, 1 ml of blood was collected by orbital blood sacrifice at sacrifice. (Glucose), total cholesterol, HDL (total cholesterol), and total cholesterol were measured using an automatic hematology analyzer (H1 system, Technicon, USA) after centrifugation at 3,000 rpm for 30 minutes. , LDL and triglyceride were analyzed, and the results are shown in Table 4 below.

Test substance Blood sugar
(mg / dL) Total cholesterol
(mg / dL) HDL
(mg / dL) LDL
(mg / dL) Triglyceride
(mg / dL) Normal diet (n = 10) 234.4 ± 8.49 110.4 ± 11.58 93.5 + 0.09 18.8 ± 0.12 78.3 ± 6.97 High-altitude mode (n = 10) 270.0 ± 0.77 ^a 159.5 ± 9.09 ^a 125.5 + 4.05 ^a 29.5 ± 3.51 ^a 97.4 ± 8.48 ^a High fat diet + soybean extract containing isoflavones + L-carnitine (n = 10) 236.4 ± 36.88 158.5 ± 12.76 126.0 ± 6.04 28.6 + 4.54 76.4 ± 12.25 ^b High fat diet + soybean extract containing isoflavone + L-carnitine + petal extract (n = 10) 213.1 ± 17.22 ^b 140.4 ± 9.3 ^b 119.9 ± 6.55 25.9 ± 1.96 ^b 69.9 ± 8.80 ^b High fat diet + soybean extract containing isoflavones + L-carnitine + tea catechin (n = 10) 241.3 ± 32.46 165.1 ± 10.64 136.0 + 24.91 32.4 ± 4.60 104.9 ± 33.63 High fat diet + Lilium extract (n = 10) 243.1 ± 17.34 166.2 ± 10.23 137.2 ± 18.23 30.4 ± 5.21 110.3 ± 25.43

^a p <0.05 vs normal, ^b p <0.05

In the results of the above Table 4, "soybean extract + L-carnitine intake group with high fat diet + soy isoflavone" + "soybean fat fat + L-carnitine + tea catechin fat diet with high fat diet + isoflavone" "High fat diet + dietary supplement group" showed no significant decrease in blood glucose and total cholesterol, especially LDL, compared to the control group (high fat diet group) Carnitine + petal extract group ". Carnitine supplemented with soybean extract containing high levels of + isoflavone + L-carnitine was significantly reduced in the neutral region compared to the control group. Especially, the soybean extract + L-carnitine + In the group fed with PEP extracts, triglyceride was decreased by 28% and showed the best effect.

[Test Example 4] Evaluation of expression of heat-related protein UCP1 in male C57BL / 6 mice

In order to investigate the effect of the composition of the present invention on the heat production mechanism of obesity animals induced by high fat diet, "normal diet", "high fat diet (control group)", "high fat diet + , Soybean extract containing isoflavone + soybean extract containing L-carnitine, soybean extract containing high-fat soybean + isoflavone + soybean extract containing L-carnitine + petal extract, high-fat soybean extract + isoflavone + L-carnitine + tea catechin ingestion group "and" high fat diet group + petal extract ingestion group "for 8 weeks. After the white adipose tissue of each experimental group was pulverized, RNA was extracted using TRIzol agent (Invitrogen). CDNA was synthesized using 1 ㎍ / 의 of RNA extracted and quantified as above and a reverse transcription system (Promega). Primers and probes (Applied biosystems; UCP1, NM_009463.2, PGC1-α, Mm00447181_m1, GAPDH and Mm99999915_q1) designed for each gene of the synthesized cDNA and UCP1, PGC1α and GAPDH Expression patterns of each gene were measured. The PCR reaction and analysis were carried out using a Rotor-Gene 3000 system (Corbett Research, Sydney, Australia), pre-denaturing at 50 ° C for 2 minutes, denaturing at 95 ° C for 10 minutes, Annealing for 15 seconds, and extension for 60 seconds at 60 DEG C were repeated 40 times. The results are shown in Fig.

4, " soybean extract containing L-carnitine + soybean extract + L-carnitine supplement group ", "soybean extract containing high- The expression of UCP1, a heat - related protein, was not observed in the high - fat diet supplement group. However, the expression of UCP1 was increased in the case of " soybean extract containing high isoflavone + isoflavone + L-carnitine + petal extract supplemented group ". Thus, the present invention confirms that the wilted extract gives a synergistic effect to the efficacy of soybean extract containing isoflavone and L-carnitine.

The compositions of soybean extract, L-carnitine and petroleum extract containing isoflavones according to the present invention can be applied to various formulations as described below, but the present invention is not limited thereto.

[Formulation Example 1] Preparation of tablet

After mixing the components listed in Table 5, tablets were prepared by tableting according to a conventional manufacturing method.

ingredient content The composite of Example 1 50 mg Corn starch 100 mg Lactose 100 mg Magnesium stearate 2 mg Vitamin C 50 mg

[Formulation Example 2] Preparation of capsules

Ingredients listed in Table 6 below were mixed according to a conventional method for producing a capsule, and filled in a gelatin capsule to prepare a capsule.

ingredient content The composite of Example 1 50 mg Corn starch 100 mg Lactose 100 mg Magnesium stearate 2 mg Vitamin C 50 mg Serine 50 mg

[Formulation Example 3] Production of liquid agent

The components listed in Table 7 below were added to purified water in accordance with the usual liquid preparation method and dissolved, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed and purified water was added thereto to adjust the total volume to 100 ml, filled in a brown bottle, .

ingredient content The composite of Example 1 100 mg Isomer 10 g Mannitol 5 g Vitamin C 50 mg Serine 50 mg maintain Suitable amount Purified water Balance

[Formulation Example 4] Preparation of health food

The composition ratio of the following vitamins and minerals mixture is comparatively mixed with a suitable ingredient for a health food, but the compounding ratio may be arbitrarily changed. After mixing, granules can be prepared and used in the manufacture of a health food composition according to conventional methods.

ingredient content The composite of Example 1 1,000 mg Vitamin mixture Vitamin A acetate 70 [mu] g Vitamin E 1.0 mg Vitamin B1 0.13 mg Vitamin B2 0.15 mg Vitamin B6 0.5 mg Vitamin B12 0.2 [mu] g Vitamin C 10 mg Biotin 10 [mu] g Nicotinic acid amide 1.7 mg Folic acid 50 [mu] g Calcium pantothenate 0.5 mg Mineral mixture Ferrous sulfate 1.75 mg Zinc oxide 0.82 mg Magnesium carbonate 25.3 mg Potassium monophosphate 15 mg Dicalcium phosphate 55 mg Potassium citrate 90 mg Calcium carbonate 100 mg Magnesium chloride 24.8 mg

[Formulation Example 5] Preparation of beverage

The ingredients described in Table 9 below were mixed according to a conventional beverage manufacturing method, and then the mixture was stirred and heated at 85 DEG C for about 1 hour. The resulting solution was filtered and sterilized in a sterile 2 l container. The solution was sealed and then stored in a refrigerator for use in the preparation of the beverage composition of the present invention.

ingredient content The composite of Example 1 1 g Citric acid 1 g oligosaccharide 100 g Plum concentrate 2 g Taurine 1 g Purified water Balance

[Formulation Example 6] Cream

The cream can be prepared according to a conventional method as shown in Table 10 below (unit: wt%).

ingredient content The composite of Example 1 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG60 hardened castor oil 2.0 Liquid paraffin 10 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 antiseptic Suitable amount Pigment Suitable amount Spices Suitable amount Purified water Balance

Claims (8)

A food composition for improving obesity containing as an active ingredient, a petal extract, a soybean extract containing isoflavone, and L-carnitine. The method according to claim 1,
Wherein the wilted extract is contained in an amount of 0.0001 to 10% by weight based on the total weight of the composition. The method according to claim 1,
Wherein the soybean extract is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition. The method according to claim 1,
Wherein the L-carnitine is contained in an amount of 0.001 to 40% by weight based on the total weight of the composition. 5. The method according to any one of claims 1 to 4,
Wherein the composition promotes heat production in the body. 5. The method according to any one of claims 1 to 4,
Wherein the composition is an oral composition for improving obesity. 5. The method according to any one of claims 1 to 4,
Wherein the composition inhibits triglyceride. A pharmaceutical composition for alleviating or treating hyperlipidemia, which comprises a petal extract, soybean extract containing isoflavone, and L-carnitine as an active ingredient.

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