KR101609436B1 - Composition including the compound derived from Hypnea japonica for prevention of hair loss or for promoting hair growth - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for preventing hair loss or accelerating hair growth, which comprises a hookworm extract. The composition comprising the extract of Kashmi Kashimu can be used in various fields such as pharmacy, cosmetics and cosmetics since it has an effect of inhibiting and preventing hair loss and promoting hair growth.

Description

[0001] The present invention relates to a composition for preventing hair loss or accelerating hair growth,

The present invention relates to a composition for preventing hair loss or accelerating hair growth, which comprises a hookworm extract.

Hypnea japonica Tanaka is yellowish brown or reddish brown with bluish purple fluorescence. It has a serpentine cord shape, irregular branching, and the end of the branch is hooked. The cortex consisted of one or two small bead-like cells, and the water-like tissue became a bract-shaped cell. The central axillary cells are cylindrical and the umbilical cells are cylindrical. The hook Kashiuomu is placed on rocks below the edge of the western coastline where the waves face the outer (outer) sea. It is 7 ~ 20cm in height and 1.5 ~ 3mm in diameter. It has a cylindrical shape and is attached to the branches of the branches to form large lumps. The branches that come out of the lumps are split like feathers and the long and short branches are almost horizontal. Thickening of the end of the brow makes it look like a hook. They are distributed in Korea (Busan, Geoje Island, Jeju Island, Geomundo), Japan, and Taiwan. There is a patent for eliminating the hangover of the extract of Kashiwiuumu (Korean Application No. 10-2008-0040049), but no effect of preventing hair loss or promoting hair growth has been reported.

The hair of the human body is about 100,000 to 150,000, and it is formed in "hair follicle". There is a nipple in the hair follicle, where small blood vessels are distributed to supply the nutrients needed to grow hair, and beside the papilla there is a bore hole that supplies the hair with shine oil. Hair follicles are composed of several different epithelial cells and dermal papilla cells (DPCs). DPCs are mesenchymally-derived fibroblasts located at the base of hair follicles and play an important role in hair growth. In particular, minoxidil has been reported to have proliferative and anti-apoptotic effects on DPCs. Similarly, several reports have shown an increase in hair growth through the proliferation of DPCs. Each hair has a different cycle and grows through anagen, catagen, and telogen, and then falls off. This cycle repeats over 3 to 6 years, with an average of 50 to 100 hairs dropping normally on a daily basis. In general, alopecia refers to an abnormal increase in the number of hair that has fallen due to a shortening of the percentage of growing hair and an increase in the number of retrogressive or resting hair among these cycles.

The causes of hair loss include male hypothyroidism, excess sebaceous glands, poor blood circulation, peroxidation, hypofunction of the scalp, genetic factors, aging, and stress. Testosterone, a type of male hormone, is activated by dihydrotestoserone (DHT) by an enzyme called 5α-reductase, which binds to a specific receptor and induces a protein that causes hair loss. . In addition, this mechanism causes excessive production of sebum, acne and seborrheic dermatitis, which can lead to hair loss with inflammation in the scalp. Alopecia is known to be caused by disease, malnutrition, aging, and hormonal imbalance. Although many studies have been conducted, the mechanism of essential hair loss is not well known. Efforts have also been made to treat hair loss. Nevertheless, so far only two drugs (finasteride and minoxidil) have been approved by the Food and Drug Administration (USA) for hair loss treatment. The 5α-reductase inhibitor, finasteride, has been used to promote hair growth in male androgenetic alopecia. Minoxidil, an anti-hypertensive agent, can promote hair growth by opening the ATP-sensitive K ⁺ -channel. However, the effects of drugs are limited and transient due to unpredictable effects and side effects. There is a need for better new treatments to prevent hair loss and promote hair growth. In the case of preparations such as minoxidil and trichosaccharide, which have been known to prevent hair loss and promote hair growth and have the effect on hair growth, there is no clear efficacy and human stability, skin irritation There is a problem of side effects, and it is urgent to develop a composition having safety and efficacy.

Accordingly, the inventors of the present invention have completed the present invention by confirming the hair loss prevention effect and the hair growth promoting effect of the extract of Gherkin Kashmiu while searching for natural substances exhibiting hair loss prevention and hair growth promoting effect with little toxicity.

It is an object of the present invention to provide a pharmaceutical composition for preventing hair loss, which comprises a hookworm extract as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for accelerating hair growth, which comprises an extract of Kashimashiiu as an active ingredient.

In addition, the object of the present invention is to provide a health functional food for preventing hair loss or promoting hair growth, which comprises an extract of Ganoderma lucidum as an active ingredient.

In order to accomplish the above object, the present invention provides a pharmaceutical composition for preventing hair loss, which comprises a hookworm extract as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for promoting hair growth, which comprises an extract of Ganoderma lucidum as an active ingredient.

In addition, in order to achieve the above object, the present invention provides a health functional food for preventing hair loss or promoting hair growth, which comprises an extract of Ganoderma lucidum as an active ingredient.

Since the extract of Kashi Koshiumu promotes the proliferation of important DPCs and fibroblasts in hair growth, it can be used in various fields such as pharmacy, health food, cosmetics and cosmetics by inhibiting and preventing hair loss and promoting hair growth. Do.

FIG. 1 is a graph showing the effect of accelerating the proliferation of DPCs according to the concentration of the extract of the barnyardgrass according to the present invention.
FIG. 2 is a graph showing the promoting effect of fibroblast proliferation of the hookworm extract of the present invention.

Hereinafter, the present invention will be described in detail.

As used herein, the term " extract " as defined in the present invention includes water containing purified water, lower alcohols having 1 to 4 carbon atoms or a mixed solvent thereof, a solvent selected from hexane, butylene glycol, propylene glycol, glycerin, ethyl acetate, ether, Includes an extract which is soluble in water or a mixed solvent of methanol.

The " hook gashuum extract " is not particularly limited as long as it is obtained by extracting the active ingredient from the hook gashuum. For example, the resultant product is obtained by putting a hook-and-neck mushroom into water or an organic solvent and eluting the active ingredient through means such as setting, stirring, pressing or heating. The present invention also includes a lyophilized product obtained by lyophilizing the liquid extract obtained as described above.

It also includes a powder obtained by pulverizing such a lyophilizate. In addition, the present invention includes all the extracted extracts regardless of the means available to those skilled in the art for extracting the active ingredient, including those extracted and then subjected to lyophilization and the like. Other examples include extracts obtained by conventional extraction methods such as extracting by hot water or room temperature, or extracts obtained by conventional extraction methods as described in Korean medicine or textbooks.

It also includes a fraction extract obtained through a Stass Otto extraction method or various column chromatography methods, which is a special extraction method for separating specific active compounds.

The hook extract may be used without any limitation, such as a collected product or a commercially available product. For example, the extract may be washed with water to remove foreign substances, and then dried and pulverized.

The extracts of the hookworms may be obtained by a conventional extraction method such as an ultrasonic extraction method, a filtration method and a reflux extraction method.

Preferably, the extract may be obtained by dissolving the grafted Kashiwumi powder in DMSO.

Herein, " hair loss " means prevention of hair loss phenomenon as described above, and " hair loss prevention " Promoting " means not only promoting the generation of new hair but also allowing the existing hair to grow healthily.

In general, "hair growth" occurs in the growing phase, and is induced by the delay from the dormancy to the growth phase and from the growth phase to the retrograde phase. The hair cycle can be divided into three main stages known as growth phase, regressor phase, and quiescent phase. During the growing season, hair growth takes place as the hair follicles grow deep into the skin with rapid cell proliferation. The next phenomenon is the retrogressive period, which is a transitional period in which disruption of cell division is prominent, during which the hair follicles gradually regress and hair growth stops. In the next pause, the regressive hair follicle contains germs with densely packed dermal papilla cells. The onset of a new growth phase in dormancy is induced by rapid cell proliferation in the abdomen, swelling of the papilla and synthesis of basement membrane elements.

Therefore, it is necessary to prevent hair loss by promoting or prolonging the growing period, that is, to prevent hair loss or to induce regrowth of hair, that is, to promote hair growth, and the composition according to the present invention is effective in preventing hair An effect of improving the existing hair such as thickening, and an effect of generating new hair. Dermal papilla cells are believed to play an important role in hair growth through interactions with mosquito cells of hair follicles, which are mesenchymal-derived fibroblasts located at the base of hair follicles (Jahoda CA, meat al ., 1984; Oliver RF, meat al ., 1986). In addition, minoxidil, which is used as a hair loss treatment, has been shown to increase growth in NIH3T3 fibroblasts with a K _ATP- channel opener ( Sanders , et al ., 1996). In addition, the composition according to the present invention can induce hair growth by activating dermal papilla cells (DPCs) and fibroblasts.

The present invention provides a pharmaceutical composition for preventing hair loss, which comprises an extract of Kashmi Koshimu as an active ingredient.

The concentration of the hook extract is preferably 0.01 to 100 占 퐂 / ml, but is not limited thereto. The hook extract of the present invention has almost no toxicity since it is extracted from a natural product.

The hookworm-like extract may be contained in the pharmaceutical composition in an amount of 0.01 to 70% by weight based on the total weight of the whole composition.

The pharmaceutical composition of the present invention may be various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories.

Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The pharmaceutical dosage forms of the compositions of the present invention may also be used in the form of their pharmaceutically acceptable salts and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable set. The salt is not particularly limited as long as it is pharmaceutically acceptable so long as it is pharmaceutically acceptable and includes, for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, , Benzenesulfonic acid, toluenesulfonic acid, and naphthalenesulfonic acid.

The composition of the present invention may be administered parenterally or orally, and may be administered in one to several divided doses so as to be administered in an amount of 0.1 to 500 mg per kg of body weight per day. The dosage for a particular patient may vary depending on the patient's body weight, age, sex, health condition, diet, time of administration, administration method, excretion rate, severity of disease, and the like.

The pharmaceutical composition according to the present invention may be formulated into oral compositions such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations such as ointments and creams, suppositories and sterile injection solutions And may be formulated in any form suitable for pharmaceutical preparations.

The composition according to the present invention can be administered to mammals such as rats, mice, livestock, humans, and the like by various routes such as parenteral, oral, and the like, and all manner of administration can be expected. For example, Or by intravenous, intramuscular, subcutaneous, intramural or intracerebroventricular injection.

Meanwhile, since the composition according to the present invention has no serious toxicity and side effects, it can be safely used for prolonged use for preventive purposes.

Further, the present invention provides a cosmetic composition for accelerating hair growth, comprising an extract of Kashiko Koshimu as an active ingredient.

The concentration of the hook extract is preferably 0.01 to 100 占 퐂 / ml, but is not limited thereto.

The composition may be used in hair cosmetics such as hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisturizing cream, Hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair moisturizers, Mousse, and hair spray. However, the present invention is not limited thereto.

The cosmetic composition may be prepared by dissolving or dispersing at least one compound selected from the group consisting of a fatty substance, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, Such as cosmetics or skin, such as fillers, sequestering agents, chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics And may contain adjuvants conventionally used in the scientific field. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

The cosmetic composition according to the present invention can be, for example, a cosmetic composition, and the external form of the cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. It may be in any form suitable for topical application, for example, as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing the oil phase in water, a suspension, a microemulsion, a microcapsule, In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. These compositions may be prepared according to conventional methods in the art. The composition according to the invention may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition of the present invention is not particularly limited in its formulation and may be, for example, softening lotion, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, Water, a pack, a powder, a body lotion, a body cream, a body oil and a body essence.

The cosmetic composition as described above may be applied to the skin, or may be applied to the skin using a micro needle or the like.

In addition, the compositions of the present invention may be made into a hair composition comprising a carrier or carrier mixture suitable for application to the hair. The carrier comprises a vehicle component commonly used in a carrier or other carrier or hair protective composition and is present in an amount ranging from about 0.5% to 99.5%, preferably from about 5.0% to 99.5%, and most preferably, About 10.0% to 90.0%. As a carrier, the solvent is selected by the copolymer to be used, regardless of whether the formulated hair composition remains on the hair after use such as hair spray, mousse, tonic, etc., or whether it is cleaned, such as shampoos, conditioners and the like. Suitable solvents for use in the present invention include water, lower alcohols (ethanol, isopropanol, etc.), hydroalcoholic mixtures, hydrocarbons such as isobutane, hexane and decene, acetone, halogenated hydrocarbons such as Freon, Dibutyl phthalate and the like), volatile silicone derivatives, siloxane (phenylpentamethyldisiloxane, methoxypropylheptamethylcyclotetrasiloxane, chloropropylpentamethyldisiloxane, hydroxypropylpentamethyldisiloxane, octamethylcyclotetrasiloxane, decamethyl Cyclopentasiloxane, and the like), and mixtures thereof.

In addition, the present invention provides a health functional food for preventing hair loss or promoting hair growth, which comprises an extract of Ganoderma lucidum as an active ingredient.

The concentration of the hook extract is preferably 0.01 to 100 占 퐂 / ml, but is not limited thereto.

The health functional food may be powder, granule, tablet, capsule or beverage, but is not limited thereto.

The food of the present invention can be used as it is, or can be used with other food or food ingredients, and can be suitably used according to conventional methods.

There is no particular limitation on the kind of the food. Examples of the food to which the hook-like Kashimu extract can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, , A drink, an alcoholic beverage, and a vitamin complex, and includes foods in a conventional sense.

The beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the food of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in a carbonated beverage, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.001 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more fully explain the present invention to those skilled in the art.

< Example  1> Manufacture of hooked Kashiwumu extract

Hypnea , a seaweed hook japonica Tanaka) was supplied as a powder form from Jeju Biodiversity Research Institute.

< Example  2> Extract of Kashiwiumu extract Dermal papilla cells ( Dermal papilla cell , DPCs ) Confirmation of proliferation promoting effect

Immortalized dermal papilla cells isolated from rat beard (Filsell W, et . al, 1994) to 100 units / ml penicillin-100 ㎍ / ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated fetal bovine serum (FBS; Gibco Inc, NY, USA) are contained DMEM (Hyclone Inc, USA) at 37 ° C in a 5% CO ₂ incubator and subcultured once every 3 days. The proliferation of dermal papilla cells was measured using MTT assay. 1.0 × 10 ⁴ cells / ml of dermal papilla cells were suspended in DMEM medium containing 1% FBS, and the cells were cultured for 24 hours in a 96-well plate. Then, the extracts prepared in Example 1 were treated with 0.1, 1 and 10 ㎍ / ml. The positive control, minoxidil (Sigma, MO, USA), was treated at a concentration of 10 μM. After 4 days of incubation, 50 μl of MTT (Sigma, MO, USA) was added and reacted for 4 hours. The supernatant was removed, 200 μl of DMSO was added to dissolve the precipitate, and the absorbance was measured at 540 nm using a VERSAmax microplate reader (Molecular Devices, CA, USA). The average absorbance value of each sample group was obtained and compared with the absorbance value of the control group, the degree of proliferation of the dermal papilla cells was examined. All measurements were expressed as mean ± SD. Statistical significance was tested by student's t test and significance was considered when p-value was less than 0.05. Statistical analysis was performed using SPSS 12.0K for Windows (Release 12.0.1, SPSS Inc. USA).

As a result, when treated with the extract of Kashii Kashiwumu at the concentrations of 0.1, 1 and 10 μg / ml, the concentrations of 110.2 ± 7.8% ( P <0.05 ), 114.0 ± 7.3% ( P <0.01 ) and 126.5 ± 8.3 % ( P < 0.001 ). In particular, at the concentration of 10 / / ml, the extract of the Kashiwiushi extract showed a higher proliferative effect than 122.3 賊 9.5% ( P <0.001 ) of the positive control minoxidil (10 袖 M) (Fig. 1). These results indicate that the extract of Kashmi Kashiumu has similar hair growth properties to that of minoxidil.

< Example  3> Confirmation of fibroblast proliferation promoting effect of extract

NIH3T3 fibroblasts were purchased from the American Type Culture Collection (ATCC, Rockville, USA) and incubated with 100 units / ml penicillin-100 ug / ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated bovine calf serum (BCS; (Gibco Inc, NY, USA) at 37 ° C in a 5% CO ₂ incubator and subcultured once every 3 days. The proliferation of NIH3T3 fibroblasts was measured using the MTT assay. NIH3T3 fibroblasts (1.0 × 10 ⁴ cells / ml) were suspended in DMEM medium containing 10% or 1.5% BCS and placed in a 96-well plate. After culturing for 24 hours, the extracts of Kashii Kashiumu were added at concentrations of 0.1, 1 and 10 μg / ml Respectively. The positive control, minoxidil (Sigma, MO, USA), was treated at a concentration of 75 μM. After 4 days of incubation, 50 μl of MTT (Sigma, MO, USA) was added and reacted for 4 hours. After the reaction, the supernatant was removed, 200 μl of DMSO was added to dissolve the precipitate, and the absorbance was measured at 540 nm using a VERSAmax microplate reader (Molecular Devices, CA, USA). The average absorbance of each sample group was calculated and compared with the absorbance of the control group. All measurements were expressed as mean ± SD. Statistical significance was tested by student's t test and significance was considered when p-value was less than 0.05. Statistical analysis was performed using SPSS 12.0K for Windows (Release 12.0.1, SPSS Inc. USA).

As a result, the extracts of Kashii Kashiwumu showed the proliferative activities of 102.0 ± 5.1%, 108.4 ± 7.3% and 119.2 ± 4.6% ( P <0.001 ) in the concentrations of 0.1, 1 and 10 ㎍ / 75 [mu] M minoxidil showed 133.6 + 4.4% ( P < 0.001 ) proliferative effect (Fig. 2). These results indicate that the extract of Kashmi Kashimu has K _ATP- channel opening effect like minoxidil.

Hereinafter, based on the results of the above-described examples, various examples of pharmaceuticals are prepared and presented. However, these formulation examples are for the purpose of illustrating the present invention, and it is obvious to those having ordinary skill in the art that the formulation of the present invention is not limited to these formulation examples

< Formulation example  1> Preparation of tablets

Gherkin Kashimu extract 1 mg

Corn starch 68 mg

Lactose 90 mg

40 mg of microcrystalline cellulose

Magnesium stearate 2 mg

According to the conventional preparation method of tablets, the above ingredients were added in the prescribed amounts, uniformly mixed, stirred, and then granulated. After drying, the tablets were used to prepare the desired tablets each containing 1 mg of the hookworm Kashiwumu extract as an active ingredient per one tablet.

< Formulation example  2> Preparation of capsule

Gherkin Kashimu extract 1 mg

Corn starch 68 mg

Lactose 90 mg

40 mg of microcrystalline cellulose

Magnesium stearate 2 mg

According to a conventional method for preparing a capsule, the above ingredients were added in the prescribed proportions and mixed uniformly. The resulting mixture was filled in gelatin capsules of appropriate size so as to contain 1 mg of glycoprotein Kashiuomu extract per capsule to prepare a desired capsule .

< Formulation example  3> Ointment in skin external medicine

1% by weight Kashiwumu extract

Glycerin 8 wt%

4% by weight of butylene glycol

Liquid paraffin 15 wt%

7% by weight of beta-glucan

Carbomer 0.1 wt%

Caprylic / capric triglyceride 3 wt%

Squalane 1 wt%

1.5% by weight of cetearyl glucoside

0.4% by weight of sorbitan stearate

1% by weight of cetearyl alcohol

Wax 4 wt%

Preservative, pigment, fragrance Appropriate amount

Purified water balance (up to 100)

According to a conventional ointment preparation method, the above ingredients were added in the prescribed amounts and uniformly mixed to prepare an ointment.

< Formulation example  4> Sanje  Produce

Gherkin Kashimu extract 1 mg

Lactose 100 mg

Talc 10 mg

Maintenance 5 mg

According to a conventional method for producing an acid, the above ingredients are mixed and filled in an airtight container to prepare a powder.

< Formulation example  5> Liquid  Produce

Gherkin Kashimu extract 1 mg

10 g per isomer

5 g mannitol

Vitamin C 50 mg

Serine 50 mg

Maintenance amount

Purified water balance

According to a conventional method for producing a liquid agent, the above components are mixed to prepare a liquid agent.

< Formulation example  6> Manufacture of hair tonic

10%

0.1% by weight of lenocinol

Menthol 0.05 wt%

Panthenol 0.2 wt%

0.1% by weight of salicylic acid

0.1% by weight of tocopheryl acetate

0.1% by weight of pyridoxine hydrochloride

Castor oil 5.0 wt%

Pigment amount

Fragrance appropriate amount

Ethanol suitable amount

According to a conventional method for producing a hair tonic, the above ingredients are mixed to prepare a hair tonic.

< Formulation example  7> Hair Conditioner  Produce

Kashiwishi extract 2.5% by weight

Cetanol 3.5 wt%

1.5% by weight of self emulsifying glycerin monostearate

2.5% by weight of propylene glycol

Stearylmethylbenzylammonium chloride (25%) 7.0 wt%

0.3% by weight of methyl paraoxybenzoate

Pigment amount

Fragrance appropriate amount

Purified Water

According to a conventional method for producing a hair conditioner, the above components are mixed to prepare a hair conditioner.

< Formulation example  8> Flexible longevity (skin lotion)

Compounding ingredient Content (% by weight) Kashiwium extract extract 0.1 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservatives, coloring and flavoring Proper amount Purified water Remaining amount (up to 100)

The softening longevity was prepared by a conventional method according to the composition shown in Table 1 above.

< Formulation example  9> Nourishing cream

Compounding ingredient Content (% by weight) Kashiwium extract extract 0.1 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservatives, coloring and flavoring Proper amount Purified water Remaining amount (up to 100)

Nutritive creams were prepared according to the compositions shown in Table 2 in a conventional manner.

< Formulation example  10> Pack

Compounding ingredient Content (% by weight) Kashiwium extract extract 0.1 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservatives, coloring and flavoring Proper amount Purified water Remaining amount (up to 100)

Packs were prepared in a conventional manner according to the composition shown in Table 3 above.

Claims (9)

A pharmaceutical composition for prevention of hair loss comprising an extract of Hypnea japonica as an active ingredient, wherein the concentration of the hook extract is 0.01 to 100 占 퐂 / ml.
delete The method according to claim 1,
Wherein the composition is a formulation selected from the group consisting of tablets, capsules, injections, creams, gels, patches, sprays, ointments, alerts, lotions, liniments, pastes and cataplasms Gt;
A cosmetic composition for preventing hair loss or hair growth comprising an extract of Hypnea japonica as an active ingredient, wherein the concentration of the hook extract is 0.01 to 100 占 퐂 / ml. Composition.
delete 5. The method of claim 4,
The composition may be used in hair cosmetics such as hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisturizing cream, Hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair moisturizers, Wherein the composition is selected from the group consisting of mousse and hairspray.
A health functional food for preventing hair loss or promoting hair growth comprising an extract of Hypnea japonica as an active ingredient, wherein the concentration of the hook extract is 0.01 to 100 쨉 g / ml. Health functional food for.
delete 8. The method of claim 7,
Wherein said health functional food is a powder, granule, tablet, capsule or beverage which is a health functional food for preventing hair loss or promoting hair growth.

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