KR101608882B1 - Cosmetic Composition Comprising Golden Peanut Extracts - Google Patents

Abstract

The present invention relates to a skin cosmetic composition comprising a golden peanut extract obtained by using water, an organic solvent, or an extraction method such as supersonic, supercritical, fermentation, or spraying methods. The golden peanut extract according to the present invention is excellent in skin antioxidative effect and cell proliferation effect and thus has excellent skin aging prevention effect. The present invention relates to a cosmetic composition containing such a golden peanut extract.

Description

Cosmetic Composition Comprising Golden Peanut Extracts Containing Golden Peanut Extract

The present invention relates to a cosmetic composition comprising a peanut extract. More particularly, the present invention relates to a cosmetic composition comprising peanut extract, and more particularly to a cosmetic composition containing peanut extract obtained by using water, organic solvent, And an extract thereof.

In general, the free radical theory that skin is aged by free radicals caused by various causes outside the body among various theories that explain skin aging is regarded as the most valid theory. Free radicals are very chemically reactive chemicals that are generated by the external factors such as ultraviolet rays, radiation, smoke, smoke, tobacco smoke, and stress in the human body and metabolic processes of mitochondria and leukocytes in the body, The peroxidation of the lipid component of the cell membrane.

Most of the free radicals associated with skin aging are Reactive Oxygen Species (ROS). They include super oxide radicals, hydrogen peroxide (H ₂ O ₂ ), hydroxy radicals, and singlet oxygen. The lipid peroxidation caused by these causes various active oxygen species of radicals, peroxides, aldehydes and epoxides and damages DNA, RNA, protein, cell membrane and cell structure.

So far, research on antioxidants has been one of the most studied areas for inhibiting skin aging. Radicals such as superoxide radicals (O ₂ ^- ) and hydroxyl radicals (OH) and non-radicals such as hydrogen peroxide (H ₂ O ₂ ) cause oxidative stress on the skin, thereby damaging cells and promoting skin aging . Therefore, not only the direct antioxidant effect on various oxidizing substances but also the suppressive effect of TNF-a and IL-8 secretion from keratinocytes suppresses oxidative stress of cells, and after that, TNF-a and IL-8 secreted from keratinocytes There have been ongoing studies on antioxidants that inhibit secondary reactions to peripheral skin cells such as Langerhans cells, fibroblasts, and melanocytes to fundamentally prevent skin aging.

These antioxidants that prevent skin damage by active oxygen include superoxide dismutase (SOD), enzymes such as catalase and tocopherol (vitamin E), beta- carotene, Lipid soluble and water soluble antioxidants or radical scavengers such as ascorbic acid (vitamin C) and glutathione.

As described above, since free radical oxygen or reactive oxygen species are highly related to skin aging, conventionally, superoxide dismutase, vitamin E derivatives, vitamin C and derivatives thereof, and flavonoids have been added to cosmetics There have been many efforts to achieve skin anti-aging effects by blending. However, when the above ingredients are blended in cosmetics, safety, stability, and the like are poor, and it is difficult to expect a substantial effect.

Accordingly, in order to solve the problems of the prior art described above, there is a continuing need for the development of products and raw materials having excellent antioxidative effect and excellent cell proliferation effect.

An object of the present invention is to provide a cosmetic composition excellent in antioxidative effect and excellent in cell proliferation effect. It is another object of the present invention to provide a cosmetic composition having an anti-aging effect through an effect of removing active oxygen in the skin and protecting the skin from cell damaging substances and enhancing collagen biosynthesis by promoting proliferation of skin cells have.

The above-mentioned problem is achieved by a cosmetic composition containing peanut (hereinafter referred to as 'golden peanut') extract grown using gold ionized water.

Preferably, the golden peanut extract is an extract of peanut fruit.

The peanut extract of the present invention is also characterized by its antioxidant effect on skin, cell migration effect and skin elasticity.

In addition, the golden peanut extract may contain 0.001 to 30.0% (w / w) based on the total weight of the cosmetic.

Preferably, the golden peanut extract is prepared by dissolving the peanut in water, anhydrous or lower alcohols having 1-4 carbons, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, dichloromethane, A supercritical extraction method, a fermentation method, and a pulverization method, which comprises extracting by using a solvent extraction method using an extraction solvent selected from the group consisting of hexane, hexane, and mixtures thereof.

The cosmetic composition may also be selected from the group consisting of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, It can have a formulation.

The present invention relates to a cosmetic composition for preventing skin aging comprising peanut extract grown using gold ionized water. The golden peanut extract, which is an active ingredient contained in the cosmetic composition of the present invention, has excellent antioxidative and cell migration effects on skin and excellent skin elasticity, and thus has excellent efficacy as a cosmetic for preventing skin aging.

FIGS. 1A to 1C show the results of measuring the antioxidative effect of the golden peanut extract of the present invention.
2 shows the cell migration effect of the peanut extract (2a), peanut extract (2b), mung bean extract (2c) and control group (3d) of the present invention.

The present invention provides a skin cosmetic composition comprising peanut extract.

The peanut ( Arachis Hypogaea ) used as an active ingredient in the cosmetic composition of the present invention is a high-calorie food rich in high-protein, high-fat and vitamin B group. It is known that peanuts contain a lot of vitamin E, which strengthens cells, increases red blood cells, and helps iron absorption. Unsaturated fatty acids in peanut fats are known to lower cholesterol levels. Also, peanuts cleanse blood vessels to clear blood, and vitamin E ingredients in peanuts are known to be effective in preventing aging by exerting an excellent effect in the prevention of arteriosclerosis.

In addition, the presence of resveratrol, an anticancer substance, in peanuts has recently been confirmed. Resveratrol is a type of phytoalexin, an anti-toxic substance that peanuts produce against external physical stimuli or fungal infestations. Phytoalexin protects itself against external biological or non-biological stimuli. It is a collective term for the substances that it produces. Resveratrol has been reported to exhibit anticancer, antithrombotic, antioxidant, and lowering the incidence of cardiovascular disease (YJ Surh et al. Cancer Letters, 140: 1-10, 1999. L Front. Life Sciences, 66: 663-673,2000). Also, the peanut used as an active ingredient in the composition of the present invention was obtained by purchasing and extracting golden peanut from Udo Mountain, Jeju Island containing gold ionized water.

In the present invention, harvested peanut (golden peanut) after the application of the gold nano-ionized water to the peanut, which is the technology described in Korean Patent Registration No. 10-0555584, 'Method for manufacturing metal nanoparticles using electrolysis method' was purchased and used. The golden peanut used in the present invention was purchased from " Golden Udo Peanut Co., Ltd. " Golden peanuts were prepared by dissolving 99.9% of gold nanoparticles of 2 nm or less in distilled water and then fertilizing them three times.

The golden peanut extract of the present invention can be prepared by conventional methods known in the art, that is, under the conditions of ordinary temperature and pressure, using a conventional solvent (e.g., water, anhydrous or hydrous lower alcohol , Acetone, ethyl acetate, butyl acetate or 1,3-butylene glycol).

According to a preferred embodiment, the method for preparing the golden peanut extract of the present invention is as follows. First, the peanut was peeled off, washed with purified water, dried and crushed into small pieces, and then water, 1 to 10 times the weight of dry weight, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, Butyl acetate or 1,3-butylene glycol. Then, it is extracted by heating at 40 to 100 ° C for 3 to 20 hours or at 4 to 40 ° C for 1 to 15 days in a state where a cooling condenser is installed to prevent evaporation of the active ingredient, and is completely dried by a rotary evaporator . In this case, in the case of 1,3-butylene glycol, it is difficult to dry using a rotary evaporator, so it is extracted directly under the above conditions and adjusted to a 1% (w / v) loss on drying.

Meanwhile, the golden peanut extract of the present invention includes extracts by supercritical extraction or ultrasonic extraction by decompression by carbon dioxide and high temperature as well as extracts by the above extraction method.

Decompression by carbon dioxide and extraction by supercritical extraction by high temperature means supercritical fluid extraction. Generally, supercritical fluids have the properties of liquid and gas when gas reaches the critical point at high temperature and high pressure, and chemically have polarity similar to that of nonpolar solvent. Due to this characteristic, supercritical fluid (J. Chromatogr. A. 1998; 479: 200-205).

Carbon dioxide is a supercritical fluid with both liquid and gaseous nature, with the operation of supercritical fluid equipment reaching its critical pressure and temperature, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the lipophilic substance contained in the sample is extracted into supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using a mixed fluid in which a cosolvent is further mixed with supercritical carbon dioxide or carbon dioxide. These co-solvents may be used alone or in admixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether.

Most of the extracted samples contain carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a reduced pressure evaporator.

As the extraction solvent which can be used for the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether can be used.

The extracted sample is recovered by vacuum filtration, and the filtrate is recovered, and the extract is removed by a vacuum evaporator and freeze-dried to obtain an extract.

In addition, the golden peanut extract of the present invention also includes an extract obtained by a conventional purification process and / or a fermentation process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, The fraction is also interpreted to be included in the golden peanut extract of the present invention. The fermentation product obtained by fermentation for 1 to 7 days while maintaining the pH of 5 to 7 under a temperature condition of 20 to 40 DEG C as a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis bacteria and mixtures thereof, It is interpreted to be included in the extract.

In addition, the golden peanut extract of the present invention includes the extract obtained by the pulverization method. Fojera is a pharmaceutical technology that changes the original properties of medicines by processing the medicines based on the oriental medicine theory. Mainly refers to the process of steaming, boiling, roasting, roasting, burning, or mixing processes. In the present invention, golden peanuts were steamed and dried to prepare an extract.

The golden peanut extract of the present invention can be prepared in a powder state by an additional process such as vacuum distillation and freeze drying or spray drying.

The golden peanut extract of the present invention is excellent in antioxidative effect and cell migration (cell migration), and thus has excellent anti-aging effect on skin.

According to a preferred embodiment of the present invention, the content of the peanut extract of the present invention is 0.0001 to 30.0% by weight, preferably 0.001 to 20.0% by weight based on the total weight of the cosmetic composition. When the total weight of the peanut extract is less than 0.0001% by weight, the effect of the peanut extract is less likely to be exhibited. When the total weight of the peanut extract is more than 30.0% by weight, there is a high possibility of causing skin irritation.

The ingredients contained in the cosmetic composition of the present invention include ingredients conventionally used in cosmetic compositions in addition to the golden peanut extract as an active ingredient and include conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And a carrier.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

Example  One. Golden peanut  Preparation of extract

100 g of dried peanuts were peeled off and powdered and finely ground using a mesh. Thereafter, 1,000 ml of 70% ethanol adjusted to a ratio of water and ethanol of 7: 3 was added and the mixture was leached at room temperature for 4 days. The solution was filtered and the filtrate was concentrated in vacuo to give a dry extract. The extraction yield was 34.8 g / 100 g. The dried extract was dissolved in 30% 1,3-butylene glycol solution and purified water to a concentration of 10% (w / v) and filtered to prepare a golden peanut extract.

Comparative Example  1. Preparation of peanut extract

100 g of dried peanuts, which had been peeled off, were pulverized and finely ground using a mesh. Thereafter, 1,000 ml of 70% ethanol adjusted to a ratio of water and ethanol of 7: 3 was added and the mixture was leached at room temperature for 4 days. The solution was filtered and the filtrate was concentrated in vacuo to give a dry extract. The extraction yield was 34.8 g / 100 g. The dried extract was dissolved in 30% 1,3-butylene glycol solution and purified water to a concentration of 10% (w / v) and filtered to prepare peanut extract.

Comparative Example  2. Preparation of mung bean extract

The mung bean was washed with purified water and dried. The dried mung bean was powdered and made fine using 300 mesh. Thereafter, 1,000 ml of 70% ethanol adjusted to a ratio of water and ethanol of 7: 3 was added and the mixture was leached at room temperature for 4 days. The solution was filtered and the filtrate was concentrated in vacuo to give a dry extract. The extraction yield was 34.8 g / 100 g. The dried extract was dissolved in 30% 1,3-butylene glycol solution and purified water to a concentration of 10% (w / v) and filtered to prepare mung bean extract.

Experimental Example  One: Golden peanut  Antioxidative effect of extract

The antioxidative effect (free radical scavenging ratio) of the peanut extract and the peanut extract of Example 1 and Comparative Example 1 was measured using the DPPH method.

The DPPH method measures the antioxidative effect by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The antioxidant effect was measured at a wavelength of 517 nm by comparing the absorbance of the DPPH reduced by the test substance (golden peanut extract) with the absorbance of the blank test solution.

As a simple measurement method

① Prepare a sample by diluting peanut extract and golden peanut extract with concentrations of 0.01%, 0.05%, 0.1%, 0.5%, 1% and 10% in ethanol 100%, ethanol 70% and purified water, respectively.

② Prepare α-tocopherol 200 μM and 400 μM as a control group.

③ Mix each sample solution 1: 1 with 0.2 mM DPPH solution.

④ Add 100ul to 96 well and react in the dark room for 30 minutes.

⑤ After 30 minutes, absorbance is measured at 517 nm with an ELISA instrument

Fig. 1 shows the result of the antioxidative effect measurement. Fig. 1 (a) shows the results when the golden peanut extract and the common peanut extract were diluted in 70% ethanol, Fig. 1 (b) diluted in distilled water and Fig. 1 (c) Respectively. FIG. 1 shows that the golden peanut extract (Example 1) significantly increased the antioxidative effect compared to the peanut extract of Comparative Example 1 in all three cases. Vitamin E (tocopherol) was used as a control, and it was confirmed that Example 1 of the present invention is superior to Comparative Example 1 in antioxidative effect.

Experimental Example  2: cell migration effect

Analysis of the promoting effect of migration of fibroblasts is one of the common methods of measuring the wound healing effect of cells. Therefore, the fibroblast HDFn cells were confluently cultured in a 24-well plate, and then cells were scraped in vitro using microtip, and the peanut extract of Example 1 and the peanut extract of Comparative Example 1 and the mung bean extract of Comparative Example 2 Was treated with 1ppm and 1000ppm of the cells, followed by reaction for 24 hours. After 24 hours, the cells migrated under the microscope were photographed and the density of cells transferred to the wounding location was determined.

Compared with the extracts of Comparative Examples 1 and 2, the peanut extracts obtained in Example 1 were compared with the extracts of Comparative Examples 1 and 2 to confirm the cell migration effect. Figure 2d shows a control group.

As a result of the migration evaluation in the HDFn cell, it can be seen that Example 1 shown in FIG. 2A shows a better cell migration effect than Comparative Example 1 (FIG. 2B) and Comparative Example 2 (FIG. 2C) After 24 hours, it can be seen that the cell migration was actively performed in the middle empty space in Example 1 compared with Comparative Examples 1 and 2 by concentration.

Experimental Example  3: Evaluation of skin elasticity improvement effect

 To confirm the effect of improving the skin elasticity of the golden peanut extract obtained in Example 1 of the present invention, a cosmetic (Formulation Example 1) comprising a composition containing a golden peanut extract was prepared and its skin elasticity improvement effect was examined The following experiment was conducted. Comparative Example 1 containing Comparative Peanut Extract of Comparative Example 1, Comparative Formulation Example 2 containing mung bean extract, and Comparative Example 3 containing no extract were prepared as shown in Table 1 below, The effect of improving skin elasticity was compared.

The cream containing the peanut extract of Golden Ripe (Formulation Example 1) and Comparative Formulation Examples 1 to 3 The skin elasticity improvement effect of the cream was evaluated as follows.

15 creams (20 to 35 years old) were applied to the right side of the face with the cream of Formulation Example 1 and the left side of the face with the creams of Comparative Formulation Examples 1 to 3 twice a day for 4 consecutive weeks Respectively.

The skin elasticity improvement effect after the completion of the experiment was measured using a skin elasticity meter (SEM 575, C + K Electronic Co., Germany) before and 4 weeks after use of the product. The results are shown in Table 2 as ΔR 8 values of a skin elasticity measuring instrument (Cutometer SEM 575). The R 8 value indicates the viscoelasticity of the skin.

As shown in the following Table 2, it can be seen that the skin elasticity improving effect of the creamer of Formula 1 containing the golden peanut extract is most excellent.

(Unit: wt%) Raw material Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3



One

Stearyl alcohol 8 8 8 8 Stearic acid 2 2 2 2 Stearic acid cholesterol 2 2 2 2 Squalane 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 Polyoxyethylene (25 mole addition) alcohol ester 3 3 3 3 Glyceryl monostearate Aster 2 2 2 2 2 Butylene glycol 5 5 5 8
3
Golden peanut extract
(Example 1) 3 - - - Peanut extract
(Comparative Example 1) - 3 - - Mung bean extract
(Comparative Example 2) - - 3 - 4 Purified water To 100 To 100 To 100 To 100

Experimental product Skin elasticity effect (ΔR8) Before use after use Formulation Example 1 0.12 0.42 Comparative Formulation Example 1 0.12 0.31 Comparative Formulation Example 2 0.12 0.28 Comparative Formulation Example 3 0.11 0.17

(n = 15, p < 0.05)

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

An antioxidant cosmetic composition containing a golden peanut extract grown using gold ionized water. A cosmetic composition for improving skin elasticity containing a golden peanut extract grown using gold ionized water. The cosmetic composition according to claim 1 or 2, wherein the golden peanut extract is an extract of peanut fruit. The cosmetic composition according to claim 1 or 2, wherein the golden peanut extract contains 0.001 to 30.0% (w / w) based on the total weight of the cosmetic. The peanut extract of claim 1 or 2, wherein the peanut is selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, di A method selected from the group consisting of a conventional extraction method, an ultrasonic extraction method, a supercritical extraction method, a fermentation method and a foaming method, which extracts by using a solvent extraction method using an extraction solvent selected from ethyl ether, dichloromethane, . ≪ / RTI > delete

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