KR101563644B1 - Streptomyces griseus KR-CS7 as a novel strain with antifungal and enzyme activity - Google Patents

Abstract

The invention which is characterized by having antimicrobial activity and activity against phytopathogenic fungi, accession No. KCTC18238P a novel Streptomyces draw three-house (streptomyces griseus ) and uses thereof.

Description

Streptomyces griseus KR-CS7 as a novel strain with antifungal and enzyme activity, which has antibacterial activity and enzyme activity against plant pathogenic bacteria,

The invention which is characterized by having antimicrobial activity and activity against phytopathogenic fungi, accession No. KCTC18238P a novel Streptomyces draw three-house (streptomyces griseus ) and uses thereof.

In order to solve the problem of food security along with the global population increase, the cultivation environment of the crop has also developed remarkably. However, crops such as wild animals, pests, and plant pathogens affect production, resulting in economic losses of up to 220 trillion won worldwide. In Korea, the three sides are surrounded by the sea. 65% of the country is a mountainous region and the land is narrow compared with the population. Recently, the cultivation area of horticultural crops has been gradually increasing by using the cultivation of facilities. , Cucumber, eggplant, pepper, strawberry, melon, tomato, melon, lettuce and leek. Organic synthetic pesticides and biological agents (biopesticides or microbial pesticides) are used as remedies for pests. Of these, organic synthetic pesticides were developed in the early 1900s and can be used to increase crop production, stabilize supply, and reduce labor force in a short period of time. However, due to the adverse effects such as soil pollution, groundwater pollution, accumulation of pesticides on agricultural products, and the emergence of resistant pathogens, the social demands of safety oriented, health oriented, and natural oriented are increasing in advanced countries in recent years, .

Therefore, since the Rio Declaration of the United Nations Conference on Environment and Development, in order to protect the global environment, abuse of chemical pesticides has been restricted and replaced with microorganisms themselves or biologically active pesticides using their functions. Biological pesticides are pesticides that are used to directly control microbes themselves or to control pests by using formulations containing them. These types of pesticides are classified into microbial insecticides, microbial fungicides and microbial herbicides. There are few toxicities, few effects on ecosystems, sustainable effects, and low investment costs. However, the efficacy of the drug is delayed, and the stability of the product and the difficulty in the production method become problems.

The microorganism preparation of the pesticide which is developed at this time shows the biological control effect by the antagonistic microorganism and the soil composting ability according to various enzyme activities. The principle of these microbial preparations has the main mechanism of action such as antibiotic action by antibiotics produced by them, and compaction through separation of residues in soil by various degrading enzymes.

Representative microbes currently registered for use as microbial agents include Bacillus sp., Candida sp., Lactobacillus sp., Lactococcus sp., Rhodobacter sp. Rhodobacter sp., Pseudomonas sp., And the like. As research on useful microorganisms continues, more and more microorganisms are registered as usable microorganisms every year. It is predicted that the annual growth rate will be maintained at 1520%, which is about 2 trillion won market until the early 2000s. Most of the strains that can be developed as a product are Fusarium solani ( Botrytis spp.), Botrytis cinerea (gray mold), Rhizoctonia solani ( constellation ), Stemphylium herbarum (rot, leaf blight), Diaporthe ambigua (stem blight), Colletotrichum acutatum (anthrax), Phytophthora cactorum (Plague), Phytophthora capsici (plague), Didymella bryoniae (vine blight disease) and the like, but it has not yet occupied a large market. As mentioned above, there is a tendency to use biopesticides for environmentally friendly agriculture. Among them, various microorganisms having antagonistic action have been studied in order to reduce damage to plant pathogens. However, various pathogenic microorganisms Microorganisms having excellent antagonistic action that can be effectively controlled have not been reported.

Under these circumstances, the present inventors have made intensive efforts to develop a microorganism having an excellent antagonistic action that can effectively control various pathogenic bacteria causing various diseases of crops. As a result, it has been found that various pathogens, especially plague, melancholic, gray mold, , The present inventors have developed a strain having excellent antagonistic activity and protease, cellulase, and? -Amylase enzyme activity in leaf blight, rot, sickle cell disease, anthrax and vine blight, and completed the present invention .

It is an object of the present invention to provide a process for the production of an antimicrobial agent having an antimicrobial activity against a pathogenic bacterium causing erosion, gray mold disease, root rot, root blight, anthrax, plague, vine blight, rot, Streptomyces three very funny (streptomyces griseus ).

Another object of the present invention is to provide a microorganism preparation for controlling plant diseases, comprising the strain, the spore of the strain, the culture of the strain, or a mixture thereof as an active ingredient.

It is another object of the present invention to provide a method for controlling plant diseases using the microbial agent.

As one embodiment for achieving the above object, the present invention provides an antimicrobial agent having antimicrobial activity against a pathogenic bacterium which causes melancholia, gray mold, root rot, root blight, anthrax, plague, vine blight, rot or leaf blight characterized, accession No. KCTC18238P of Streptomyces draw three-house (streptomyces griseus ).

The term "Streptomyces" in the present invention is a representative strain of soil actinomycetes and produces antibiotics. Streptomyces belonging to the actinomycetes belong to the family Streptomyces, It is a collective term for a group of people who have.

The term "Streptomyces griseus" in the present invention refers to a species belonging to the genus Streptomyces, which grows best at alkaline pH, produces gray-yellow converted pigment and forms grayish yellow spores when forming colonies. Which is a species with morphological and physiological characteristics.

In the present invention, using the JEONBUK Jeongeup for 1 hour pre-treatment at 100 ℃ to collect good soil ability decomposed in Naejangsan and, 0.85% NaCl in order to identify a microorganism that is excellent in antibacterial activity and the enzyme activity used as a biological pesticide to 10. ⁵ The strains were isolated using an oatmeal medium supplemented with antibiotics, a selective actinomycete culture medium developed by our laboratory. After that, pure water was separated from bennett's medium and stored at -80 ° C freezer. Then, the cells were cultured for 7 days using GSS medium, centrifuged at 10,000 rpm for 15 minutes, and the supernatant was filtered with a 0.25 μm filter to separate the culture medium and cells. Thereafter, a hole was drilled in the PDA medium, and the purified culture was dispensed in an amount of 100 쨉 l each. After that, 100 쨉 l of the purified culture was dispensed, and the mixture was subjected to centrifugation at 50 르 / The strain was cultivated in Riccum aquatum, P. thophilus, Tomato, Phytophthora capsidis and D. melanifolia to isolate strains having excellent antimicrobial activity against all of the strains. Finally, strains superior in protease, cellulase, and alpha-amylase activity were finally selected for LB medium using skimmed milk powder, carboxy-methyl-celluloses and Soloblastase. Streptomyces strains Were identified as new strains belonging to Suse. Therefore, the present inventors named the strain as Streptomyces griseus KR-CS7 and submitted it to the Korea Collection for Type Culture (KCTC), an international depository institution under the Budapest Treaty, on Jan. 30, 2013 And received KCTC18238P as a trust number.

In accordance with one aspect of the present invention, the Streptomyces draw three mouse KR-CS7 strain of the present invention is Fusarium solani (Fusarium solani , Botrytis < RTI ID = 0.0 > cinerea , Rhizoctonia solani , Stemphylium herbarum), Deirdre O Bhikkhus podeo cancer (Diaporthe ambigua , Colletotrichum between acutatum), pie Saratov seashell kaek torum (Phytophthora cactorum), pie Saratov seashell kaepsi (Phytophthora capsici and Didymella < RTI ID = 0.0 > bryoniae strains (Figures 1 to 9).

The Fusarium solani is a causative organism of mosaic disease and root rot disease. Bortritis cinerea is a causative organism of gray mold, Riyotonia solanii is a causative agent of drowning and root rot, and Stempylium herbarum is a causative organism of rot and leaf Diaphora amibigua is a causative organism of the stalks, Colletoricum acutatum is a causative organism of anthracnose, Pitostaras chamomile and Phytophthora capsaceae are causative bacteria of the plague, The strain Streptomyces griseus strain KR-CS7 of the present invention is useful as a biological control agent against drowning, gray mold, pollinosis, root rot, stem blight, anthracnose, plague, vine blight, rot or leaf blight I have.

In addition, according to one embodiment of the present invention, the strain of the present invention produces a protease that is a protease, a cellulase that is a fibrinolytic enzyme, and an alpha-amylase that is a starchase, as well as a biocontroling effect (FIG. 10) .

Therefore, the Streptomyces griseus strain KR-CS7 of the present invention is a strain that can be usefully used as a biocidal pesticide for environmentally friendly agriculture, having both a biological control effect and soil composting effect through enzyme activity.

Here, the biological pesticide is defined as a commercial product of microorganisms isolated and collected in a natural environment in order to control plant pathogenic bacteria of crops.

On the other hand, peach blight, root rot, gray mold, melancholy, rot, leaf blight, stem blight, anthrax, plague and vine blight are the major diseases causing crop damage.

Plague is a major disease that affects all parts of the crop, such as roots, stems, leaves, and fruits, throughout its entire life cycle. Phytophthora ( Phytophthora) cactorum ), Phytophthora capsici) is so strong that when the pathogenic ratio produces a lot of damage and breaking on agricultural crops in a short period of time (Kang HJ et al., Res . Plant Dis. (2011) 17 (1), 1-12. Lim YS et al., Korean J. Plant Pathol. (1998) 14 (6), 735-737).

In addition, the anthrax of the plant is caused by Colletotrichum acutatum , and it destroys the leaves, stems, fruits, flowers and so on of the crops. In the case of pepper crops, which is an indispensable seasoning vegetable in Korean food, (Han KS et al., Res. Plant Dis. (2009) 15 (2), 88-93).

Root rot disease is a symptom in which part or all of the roots of a plant are decomposed or rotted, and its pathogen Rhizoctonia solani and Fusarium solani are damaged not only because the internal structure of the root is destroyed, but also because it is low in the ability of the root to absorb the root, so that the leaf is dropped and the fertilization rate is remarkably lowered (Kim WG, Korean J Plant Pathol. (1996) 12 (1), 21-32, Jeon HM, Isolation and Identification of Sickle Root Rot of Plants (2012) Master's Thesis, Sunchon National University).

Gray mold fungus is caused by Botrytis cinerea . It is a common disease occurring in the cultivation of plants. Generally, fruits and vegetables among crops are often caused by flower part after flowering, resulting in a great loss of fruit (Cheong SS et al., Kor J. Mycol. (2013) 41 (1) 38-41).

Aphrodisiac is a soilborne disease that can be caused by Rhizoctonia solani , in which part of the plant, for example the stem part, is constricted.

It is known to be a disease that can occur in almost all crops, especially in young seedlings that have germinated and have fallen to the root of the trunk of the edible grave. Fusarium solani ( Fusarium solani ) is known as a causative organism.

Stem blight disease is a disease that occurs in the main stem or branch of a tree and gradually becomes irregular with a dark brown lesion on the surface at first. The diseased part is depressed or swollen and the branch is dead. It is called. Staphylococcus is a diaphora ambigua ) are known to cause parasitism.

Vine blight disease is a plant disease caused by acanthosis, and occurs in leaves, stems, fruits, or fruits of watermelons, melons, melons, cucumbers, The leaves initially have small brown spots, and when they develop, they are enlarged to a large brown lesion (1 to 2 cm) of irregular shape. Small white spots appear on the stems and stomachs. When the stems grow, the lesions are enlarged along the stems. Severe stems all over and lesions form small black spots. As a representative pathogenic bacterium of the vine blight disease , Didymella bryoniae is known. A rot disease is an irregular rotting discoloration of a diseased part, which can occur as parasitism of Stemphylium herbarum . Leaf blight is a botanical disease that manifests as a wilting symptom of the leaf, and may appear particularly in onion, garlic, and onion by Stemphylium herbarum .

As described above, since the strain of the present invention exhibits a strong antagonistic action against pathogenic bacteria causing various plant diseases including plague, anthracnose and root rot disease, when the strain is used as an environmentally friendly non-pesticide biological pesticide, Destruction and toxicity problems, and can reduce the financial burden on each farm. In addition, due to the nature of the microorganism, it can be sustained and its long-term benefits can be seen in the future.

In another embodiment, the present invention relates to a microbial agent for controlling plant diseases, comprising the strains Streptomyces griseus, Accession No. KCTC18238P, the spore of the strain, the culture of the strain, or a mixture thereof as an active ingredient to provide.

The strain No. KCTC18238P having the above-mentioned Streptomyces griseus strain is as described above, and the strain is Fusarium solani solani , Botrytis < RTI ID = 0.0 > cinerea , Rhizoctonia solani , Stemphylium herbarum), Deirdre O Bhikkhus podeo cancer (Diaporthe ambigua , Colletotrichum acutatum ), Phytophthora ( Phytophthora cactorum ), Phytophthora capsici and Didymella < RTI ID = 0.0 > bryoniae ), it can be used for the control of plant diseases caused by the bacterium. In addition, the microbial agent may be used as an overall microbial agent such as soil improving agent, but is not limited thereto, in addition to being used as a plant disease controlling agent.

In particular, the actinomycetes Streptomyces griseus KR-CS7 according to the present invention can reduce damages caused by contamination of plant pathogens that cause problems in cultivation of crops, and has various enzyme activities, Do.

The damage caused by the contamination of the plant pathogenic bacterium is caused by the fungus such as Fusarium solani, Borotitis cinerea, Raietonia solani, Stempylium herbarium, Diaphora ambuchea, Colletorum acutumum, Lt; RTI ID = 0.0 > Diatomella < / RTI >

In addition, as long as the antibiotic activity against the microorganism is exhibited, not only the microorganism but also the spore of the microorganism, the culture of the microorganism, or a mixture thereof may be included in the microorganism preparation.

In the present invention, the term "spore" refers to a reproductive cell such as a bacterium. For the purpose of the present invention, the spore refers to a reproductive cell of Streptomyces griseus KR-CS7. In the present invention, the sporozoites of Streptomyces griseus KR-CS7 are selected from the group consisting of fusarium solani, botrytis cinerea, ryotonia solani, stamphylium herbarum, diaphora ambugua, , Phytosphaerosomes, pyothorosaccharide capsicum, and didymela bryoniae, and can be used as a microbial agent for plant disease control.

In the present invention, the term "culture product" means a product obtained by culturing the strain, and includes cultured strains or both cultured strains.

On the other hand, the strain of the present invention can be used as a probiotic agent, preferably in the form of a liquid probiotic agent or a live bacterial powder.

At this time, the probiotic and live bacterial powder containing the actinomycetes Streptomyces griseus KR-CS7 of the present invention may further include an excipient and a diluent. Streptomyces griseus strain KR-CS7 may be used alone or in combination with another extract , Can be used together with by-products.

Streptomyces Streptomyces griseus strain KR-CS7 according to the present invention may be formulated in the form of a culture solution or powder according to a conventional method. In the present invention, excipients and diluents that may be contained in Streptomyces strains are lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, Such as calcium phosphate, calcium silicate, cellulose, microcrystalline cellulose, methylcellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, stevia extract, mineral oil and natural material extract .

The microbial formulation according to the present invention is preferably diluted 1,000 to 2,000 times with water to be sprayed on the side surface and rooted, but not limited thereto.

In another aspect, the present invention provides a method for controlling a plant disease using the microbial agent. Specifically, the present invention provides a method of controlling a plant, comprising introducing the microbial agent into a plant or a growth environment of the plant.

The above microbial preparation and control are as described above.

Preferably, the plant disease may be plague, anthracnose, melancholia, pollen, leaf blight, vine blight, stem blight, rot, gray fungus or root rot.

It is preferable that the above-mentioned microorganism preparation is sprayed by using the foliar treatment method and the root of the plant roots at the ground surface of the plant at the time of clearing the soil before the preparation of the crop, 15 days after the planting, or just before the rainy season and the onset of the rainy season.

The present invention shows excellent antimicrobial activity and growth inhibitory effect on plant pathogenic microorganisms which cause plague, anthracnose, melancholia, moss green leaf, leaf blight, vine blight, stem blight, rot disease, gray mold disease or root rot disease, The enzyme activity of alpha-amylase is excellent and affects soil composting ability. The Streptomyces strain Streptomyces griseus strain KR-CS7 is useful as a prophylactic and live bacterial powder, and is useful as an environmentally friendly microorganism preparation capable of preventing destruction of ecosystems.

Figure 1 shows the antimicrobial activity of Streptomyces griseus KR-CS7 of the present invention against Fusarium solani .
Figure 2 shows the antimicrobial activity of Streptomyces griseus KR-CS7 of the present invention against Rhizoctonia solani .
Figure 3 shows the antimicrobial activity of Streptomyces griseus KR-CS7 of the present invention against Botritis cinerea .
Figure 4 shows the antimicrobial activity of Streptomyces griseus KR-CS7 of the present invention against Stemphylium herbarum .
Figure 5 shows the antimicrobial activity of Streptomyces griseus KR-CS7 of the present invention against Diaporthe ambigua .
Figure 6 shows the antimicrobial activity of Streptomyces griseus KR-CS7 of the present invention against Colletotrichum acutatum .
Figure 7 shows the antimicrobial activity of Streptomyces griseus KR-CS7 of the present invention against Phytophthora cactorum .
Figure 8 shows the antimicrobial activity of Streptomyces griseus KR-CS7 of the present invention against Phytophthora capsici .
Figure 9 shows the antimicrobial activity of Streptomyces griseus KR-CS7 of the present invention against Didymella bryoniae .
Fig. 10 shows the enzymatic activity of Streptomyces griseus KR-CS7 of the present invention on protease, cellulase, and alpha-amylase.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

Example  1: Isolation of strains used

In the present experiment, an example of the present invention is shown in Table 1. In the present experiment, there was shown a case in which, in the case of Fusarium solani, Bortithis cinerea, Raiotonia solani, Stempylium herbarum, Diaphora ambuchea, Colletorum acutum, Soil samples were collected from Busan, Jeongeup, Jeonbuk Province to isolate microorganisms having antimicrobial activity. Samples were collected at depths of about 15 centimeters from the topsoil, and weeds and dead leaves were removed. This was sealed in a zipper bag and then transported to the laboratory at 0 to 4 ° C. 1 g of the sample was treated at 100 ° C for 1 hour and then diluted to 10 ⁵ times with the diluted soil agar plate method. 0.1 ml of the diluted solution was added to an oat meal medium supplemented with antibiotics, The colonies were cultured in a 28 ° C incubator for 7 days, and the resulting colonies were transferred to Bennett's solid medium (Table 1) and stored at -80 ° C. The test strains were selected from the group consisting of Fusarium solani, Botrytis cinerea, Riyotonia solani, Stempylium herbarium, Diaphora ambugua, Colletorum acacutum, Ddimmela bryoniae were used and the most excellent strain showing strong antibacterial activity was selected in 9 strains. The strains of the selected strains formed circular colonies in bennett's solid medium and appeared white to light red pigment over time.

Medium component Concentration (g / L) Beef Extract 1.0 Yeast Extract 1.0 Glucose 20.0 Peptone 2.0 Agar 15.0

Example  2: Identification of isolated strains

The nucleotide sequence of 16S ribosomal RNA was analyzed to confirm the phylogenetic location of the strains selected in Example 1 above. The selected strains were PCR amplified using modified colony PCR method. The primers used for 16S rRNA amplification were amplified with 27F (5'-AGAGTTTGATCCTGGCTCAG-3 ', SEQ ID NO: 1), 1492R (5'-GGTTACCTTGTTACGACTT-3', SEQ ID NO: 2), and then PCR product purification kit ) Was used to purify the amplified product and the nucleotide sequence was analyzed using a DNA sequencer 3730xl (applied biosystems).

The 16S rDNA of the final selected strain was analyzed for homology in the databases of DDBJ / NCBI / Genebank and Ribosomal database project. As a result, high homology with Streptomyces griseus was confirmed. In addition, the results, the cell fatty acid composition was analyzed cells fatty acid composition was C _16: exhibited a _{1 w7c (17.69%), C} 10:: 1 w7c (32.88%), C 16: 0 (22.44%) and C _{18 0} 3OH, C _{12 : 0} 2OH, C _{12 : 0} 3OH, and exhibited a folate composition similar to that of the standard strain, indicating that the strain was a Streptomyces griseus strain.

Based on the results of analysis of the sequence and physiological characteristics, the strain of the present invention was identified as a novel strain having high homology with Streptomyces griseus, which was named Streptomyces cerevisia KR-CS7, (KCTC) on January 30, 2013, and received KCTC18238P as a deposit number. KCTC18238P has been deposited with the Korea Collection for Type Culture (KCTC) on November 1, 2013, located in Science Route 125, Yuseong-Gu, Daejeon.

Example  3: Antimicrobial activity measurement against various plant pathogenic bacteria

Plant pathogens include, but are not limited to, Rhizoctonia solani AG-2-1 KACC 40123 (constipation), Botrytis cinerea KACC 40573 (gray mold), Fusarium solani KACC 40384 (rootstock, root rot), Diaporthe ambigua KACC 41219 (Stalks blight), Colletotrichum acutatum KACC 40689 (anthrax), Phytophthora cactorum KACC 40166 (Plague), Didymella brtoniae KACC 40669 (vine blight), Phytophthora capsici KACC 40475 (Plague), Stemphylium herbarum (rot, leaf blight), take pre-sale test bacteria of a total of nine species in the Microbiological Resource Center South Korea Agriculture investigated the antibacterial activity of a range of Streptomyces three very funny KR-CS7.

Specifically, each pathogenic bacterium was cultured in a potato agar medium (PDA, Difco) and used as a seed bacterium. The culture of the isolated strain grown in the GSS medium (Table 2 below) was centrifuged and filtered with a 0.25 μm filter Respectively. Then, a pre-treated culture broth was poured into a hardened potato agar broth with a diameter of 5 mm, and each pathogenic bacterium was cultured and cultured at 25 ° C for 3 to 7 days. At this time, the effect of the antagonistic microorganism on various pathogens was examined with or without support, and the results are shown in Table 3 and FIGS. 1 to 9.

Medium component Concentration (g / L) Soluble starch 10.0 Glucose 20.0 Yeast Extract 4.0 Beef Extract 1.0 NaCl 2.0 K ₂ HPO ₄ 0.25 CaCO ₃ 2.0 Soy bean 25.0 Agar 15.0

As a result, as shown in Table 3 and FIG. 1 to FIG. 9, the strain of the present invention showed excellent antibacterial activity against all of the tested nine strains, and Fusarium solani KACC 40384 (rootstock, root rot), Diaporthe ambigua KACC 41219 (stem blight), Colletotrichum acutatum KACC 40689 (anthrax), Phytophthora The antimicrobial activity of the capsici KACC 40475 ( pest ) and Stemphylium herbarum (rot, blight) was particularly excellent.

Example  4: Isolate  Enzyme activity measurement

The enzymatic activity of the isolated strain was measured by measuring the size of the transparent ring by adding 1% skim milk to the LB medium and measuring the protease activity. Cellulase activity was measured by adding 0.5% of CMC to the LB medium for 24 hours After staining with 0.2% Congo-red for 15 min and destaining with 1 M NaCl, the size of the yellow ring was measured. The α-amylase activity was determined by measuring the size of the yellow circle formed by dropping Rugol's solution for 24 hours in a medium containing 1% soluble starch. The results are shown in Tables 4 and 10 below.

Strain Enzyme activity (mm) Protease Cellulase α-amylase Isolated strain 12 13 8

As a result, the strain of the present invention showed excellent results in protease, cellulase, and amylase activity.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Korea Biotechnology Research Institute KCTC18238P 20130130

<110> Korea Research Institute of Bioscience and Biotechnology <120> Streptomyces griseus KR-CS7 as a novel strain with antifungal and          enzice activity <130> PA131318KR <160> 2 <170> Kopatentin 2.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 27F Primer <400> 1 agagtttgat cctggctcag 20 <210> 2 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> 1492R Primer <400> 2 ggttaccttg ttacgactt 19

Claims (8)

Fusarium solani , Botrytis cinerea , Rhizoctonia solani , Stemphylium herbarum , Diaporthe ambigua , Cholerae , It has an antimicrobial activity against all pathogenic bacteria consisting of Colletotrichum acutatum , Phytophthora cactorum , Phytophthora capsici and Didymella bryoniae . Accession No. KCTC18238P Streptomyces griseus strain.
The strain according to claim 1, wherein the strain has a controlling effect against drought, gray mold, root rot, root blight, anthracnose, plague, vine blight, rot or leaf blight.
The strain of claim 1, wherein the strain exhibits protease, cellulase, and amylase activity.
A fusarium solani ( Fusarium solani ), a Botrytis cinerea ( Bacterium sp. ) Containing the strain of any one of claims 1 to 3, a spore of the strain, a culture of the strain, cinerea , Rhizoctonia solani , Stemphylium herbarum , Diaporthe ambigua , Colletotrichum acutatum , Phytophthora ( Phytophthora bacterium ), Rhizoctonia solani , a microbial agent for controlling plant diseases caused by one or more pathogens selected from the group consisting of cactorum , Phytophthora capsici , and Didymella brioniae .
delete 5. The microbial formulation according to claim 4, wherein the plant disease is at least one selected from the group consisting of melancholia, gray mold, root rot, root blight, anthracnose, plague, vine blight, rot, and leaf blight.
The microbial formulation according to claim 4, wherein the microbial agent exhibits a soil compaction effect.
A method for controlling a plant disease, comprising introducing the microorganism preparation of claim 4 into the plant or the growth environment of the plant.

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