KR101469797B1 - Cosmetic composition for anti-aging containing swallow's nest extract made by using colloidal gold solution as a solvent - Google Patents

Abstract

The present invention relates to a cosmetic composition containing colloidal gold-bird's nest extract extracted with colloidal gold solution as an active ingredient. The colloidal gold-bird's nest extract has an effect of inhibiting the expression of an inflammatory mediator, an increase in expression of a moisturizing factor, and an excellent skin irritation-mitigating effect.

Description

[0001] The present invention relates to an anti-aging cosmetic composition containing colloidal gold bird's nest extract as an active ingredient,

The present invention relates to a cosmetic composition containing colloidal gold-bird's nest extract extracted with a colloidal gold solution as an active ingredient. More specifically, the present invention relates to a cosmetic composition comprising a colloidal gold solution as a solvent, To a cosmetic composition containing the same.

The cause of skin aging is divided into extrinsic factor and endogenous factor. Exogenous factor that aging occurs when exposed to ultraviolet rays or environmental stimuli. Aging phenomenon that occurs naturally when aged is called endogenous factor. Although the aging reaction due to double endogenous factors varies, the theory of degradation, oxidation, and glycation of collagen synthesis arising from aging has been raised, among which saccharification is emphasized. Glucose is a phenomenon in which sugar flows along blood vessels and changes in properties due to binding with proteins in the body. The final oxide produced by glycation is called the Advanced Glycation End product (AGE), which is stiff, unlike tough, flexible collagen or elastin. At this time, when the collagen or elastin is abundant in the dermis layer, the junction of the dermis and the epidermis has a serpentine shape, but since it changes into AGE, it is flattened so that the area supplied with moisture and nutrition is reduced, Skin tone and other signs of aging. Therefore, anti - glycation has become an important keyword of anti - aging, and the development of materials based on our scientific research is increasing.

Other factors that accelerate aging include water deficiency and skin inflammation. Although 60-70% of the human body is made of water, the external stimulation causes the moisture to be lost to the air, causing the skin to lose moisture, which causes skin aging such as lack of water and skin elasticity. An inflammatory response is a physiological response that protects the body from factors that are harmful or harmful to the tissue. It is caused by proteolytic enzymes caused by excessive inflammation, damaging cells and connective tissues, reducing skin elasticity and causing wrinkles, resulting in rapid skin aging. Therefore, the skin aging can be suppressed by protecting the damaged cells by suppressing the proteins and lipolytic enzymes produced by excessive inflammation.

Korean Patent No. 1252149 entitled " Anti-aging cosmetic composition "discloses a cosmetic composition that exhibits an anti-aging effect through inhibition of final glycation end products including sea grape and auditory mixed extracts. On the other hand, Korean Patent Laid-Open Publication No. 2011-0116801 entitled " Composition for improving skin condition containing an extract of birds as an active ingredient ", discloses a composition for improving skin condition comprising bird's nest extract as an active ingredient.

The inventors of the present invention studied a variety of natural products having an antiglycosylation activity while preparing a colloidal gold solution and using the colloidal gold solution as a solvent to study the activity thereof. The colloidal gold- And moisturizing activity. Thus, the present invention has been completed.

It is an object of the present invention to provide a cosmetic composition which exhibits an excellent anti-aging activity by containing a bird's nest extract extracted with colloidal gold solution as an active ingredient.

Another object of the present invention is to provide a method for producing a bird's nest extract having excellent anti-aging activity using colloidal gold solution as a solvent.

To achieve the above object, according to the present invention, there is provided a cosmetic composition comprising a bird's nest extract extracted from colloidal gold solution as a solvent as an active ingredient. Wherein the colloidal gold solution contains gold particles in a concentration of 1 to 10000 ppm.

The colloidal gold solution used as an extraction solvent comprises: A) dissolving gold salts in a solvent to prepare a gold solution; B) reducing the reducing agent by adding a reducing agent; C) cleaning and drying to produce gold particles; And D) dissolving the gold particles in a solvent. The gold salts may be selected from the group consisting of gold chloride, gold bromide, gold sulfide, chloroauric acid, gold hydroxide, At least one selected from the group consisting of chlorotriethyl phosphine gold and chlorotriphenylphosphine gold may be used. The reducing agent may be at least one selected from the group consisting of ethylene oxide, sodium borohydride, sodium citrate, thiocyanate, and ascorbic acid. Is used.

In the steps A) and D), additives such as polyvinylpyrrolidone (PVP), sodium dodecyl sulfate (SDS) and / or polyvinyl alcohol (PVA) are mixed together Can be used.

The bird's nest extract extracted from the colloidal gold solution as a solvent may be contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic. The cosmetic composition containing the colloidal gold-bird's nest extract obtained by using the colloidal gold solution as a solvent has anti-glycation activity and inhibitory activity on the skin inflammatory mediator expression. In addition, the cosmetic composition increases the moisturizing power of the skin by increasing the expression of a moisturizing factor and exhibits a skin irritation mitigation effect.

According to another aspect of the present invention, there is provided a method for preparing a colloidal gold solution, comprising the steps of: A) B) extracting at a low temperature of 4 to 50 캜 for 3 days; And (C) adding a polyol to remove impurities, and then aging the mixture by immersing the mixture at a low temperature of 4 to 50 DEG C for about 3 to 4 days, thereby producing a colloidal gold bird's nest extract.

The colloidal gold-bird's nest extract prepared by extracting a bird's nest with the colloidal gold solution according to the present invention is excellent in anti-glycation effect, anti-inflammatory effect and moisturizing effect, and thus the cosmetic containing the extract as an active ingredient has excellent anti- It can be usefully used as a cosmetic for improvement.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition containing a bird's nest extract prepared by using colloidal gold solution as an extraction solvent as an active ingredient.

There is a fine current in gold. Gold is harmless to the human body and is excellent in electric conduction. When pure gold is applied to the skin, it regulates the movement of bioelectric current and smoothes the skin physiological function. It acts on the electrical signal that governs the heartbeat, promoting blood circulation, and simultaneously helping the veins and lymphatic system to quickly wipe out waste products. When blood passes through the lungs, hemoglobin binds with oxygen to carry oxygen to the capillaries of the dermis. This process also combines gold microcurrent and excellent oxygen transfer ability to promote the circulation of the skin and help the skin to regenerate quickly.

In order to utilize the gold in the form of particles, the gold can be electrolytically decomposed in a solution or chemically reduced to form a colloid having a small particle size of gold. The gold can be used by being adsorbed on silica or zeolite.

Colloidal gold is a colloidal state of high-purity gold in a solvent and exists in an ionic form. Colloidal gold facilitates particle size control and facilitates phagocytosis. In addition, gene peptides and proteins used for therapeutic purposes exist in the form of ions and are hydrophilic materials, so they can be easily dissolved in water to form complexes with existing colloidal gold.

The swallow's nest is a nest of sea-swallows called Collocalia fuciphaga . Nesting on a coastal cliff, it nests a nest that has not been digested yet. At this time, the bird's nest is white and shiny. The bird's nest is mainly known as seaweed and fish fins. The ingredient is known as a ginseng protein, which is known as a ginseng ginseng ceremony. It is said to be "beneficial to blood and vitalize in skin". In addition, the bird's nest is excellent for the rejuvenation effect, and the Chinese embryo was used for skin beauty by mixing sea birds and pearl powder to maintain their beauty and youth. The bird's nourishment contains protein that nourishes the skin, giving it a more gentle treatment for dry skin, and contains vitamin C, vitamin E, arbutin and collagen to give the skin a tired look and keep it healthy and resilient. Proteoglycan, a major component in the bird's nest, plays a major role in binding or supporting individual cells such as whole body, skin, and organs. Thus, it has excellent regenerating effect on the skin, It plays a major role in

In the present invention, a bird's nest extract using colloidal gold solution as a solvent is used as an active ingredient of the cosmetic. In this case, the antioxidant activity was superior to that of the common extract of the bird by the synergistic action of the gold component and the active ingredient of the bird 's nest.

According to the present invention, there is provided a cosmetic composition comprising, as an active ingredient, a bird's nest extract extracted from colloidal gold solution as a solvent. As the colloidal gold solution as an extraction solvent, gold particles having a concentration of 1 to 10000 ppm can be used.

According to one embodiment of the present invention, the colloidal gold solution used as an extraction solvent can be prepared by the following method.

First, an aqueous gold solution is prepared by dissolving gold salts in a solvent, preferably water. Then, a reducing agent is added to reduce gold ions. Then, it is washed and dried to prepare gold particles. The gold particles thus prepared are dissolved in a solvent, preferably water, to prepare a colloidal gold solution. At this time, the gold salts include gold (III) chloride, gold bromide, gold sulfide, chloroauric acid, gold hydroxide gold hydroxide, chlorotriethyl phosphine gold, and chlorotriphenylphosphine gold may be used. The reducing agent may be at least one selected from the group consisting of ethylene oxide, sodium borohydride, sodium citrate, thiocyanate, and ascorbic acid. Can be used.

According to one embodiment of the present invention, the colloidal gold solution thus prepared is used as an extraction solvent to prepare a bird's nest extract according to the following method.

 First, a colloidal gold solution with a weight of 5 to 10 times is added to a chopped bird's nest after drying, and the mixture is extracted for 3 days at a low temperature of 4 to 50 ° C. It is preferable to extract at low temperature to minimize the alteration of the proteoglycan, an active ingredient of the bird's nest. Further, in order to increase extraction efficiency, it is possible to carry out extraction stabilized by decompression (vacuum).

Next, the polyol is added to remove the impurities, and the mixture is aged by immersing at low temperature of 4 to 50 ° C. for 3 to 4 days.

The bird's nest extract extracted from the colloidal gold solution as a solvent may be contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic.

The colloidal gold-bird's nest extract of the present invention thus prepared and the cosmetic composition containing the colloidal gold-avian extract of the present invention were superior in antagonism activity (Test Example 1) And the effect of increasing the expression of factors related to moisturization (Test Example 3).

The cosmetic composition containing the colloidal gold-bird's nest extract having anti-glycation, anti-inflammation and moisturizing effect of the present invention can be prepared in any formulations which are conventionally prepared, and examples thereof include lotion, cream, essence, cleansing foam, Body lotion, body oil, body gel, shampoo, rinse, hair conditioner, hair gel, foundation, lipstick, mascara, make-up base and the like.

[Example]

Hereinafter, the present invention will be described in detail by way of the following examples and test examples, but the present invention is not limited to these examples.

Example 1: Preparation of colloidal gold solution

Gold (III) chloride is dissolved in water to a concentration of 0.01 wt% to prepare an aqueous solution. Ethylene oxide is then added in an amount of 0.00075 wt% based on the weight of the aqueous solution. The precipitate was filtered, washed and dried, and gold particles were dissolved in water 10 times by weight to obtain a colloidal gold solution (1000 ppm).

Example 2: Preparation of colloidal gold-bird's nest extract

The colloidal gold solution of Example 1 was used as the extraction solvent of the bird's nest. After drying, the colloidal gold solution (5-fold weight) was added to 50 g of the chopped bird's nest and the mixture was extracted at a low temperature of 40 캜 for 3 days. To remove impurities, 5 g of Butylene Glycol was added and the mixture was immersed for 4 days at 40 ℃ for aging. The concentrated colloidal gold-virgin extract 38.7 g.

Comparative Example 1: Production of bird's nest extract

As the extraction solvent of the above-mentioned chopped bird's nest, the weight of 5 times 70% ethanol was added, and the mixture was boiled at 100 ° C for 8 hours in an extractor equipped with a cooling condenser. After filtering, the mixture was filtered through a 300-mesh filter paper, left at room temperature for 3 days, and the precipitate was filtered twice using filter paper Edbentek No. 5 and Watman GFC 47 mm filter paper. The mixture was concentrated at 50 ° C using a vacuum concentrator, and then dried using a spray dryer (manufactured by BUCHI) to obtain 20.9 g of a bird's nest extract.

Test Example 1: Experiment to measure the anticarcinogenic effect

When the glycation reaction proceeds, a series of complex processes such as oxidation and condensation are performed to produce final glycation products (AGEs) in which various compounds are mixed. Some of the final glycation products are fluorescent, and are used as a measure of the degree of glycation reaction. Among them, fluorescence at 370nm / emission 440nm is generally known as the degree of glycosylation. The experiment was conducted using the above principle. Preparation of the reaction was carried out by modifying the method of Mcpherson et al. (Role of fructose in glycation and cross-linking of proteins. Biochemistry 1998, 27, 1901-1907). (BSA, 5 mg / ml), 25 mM ribose, 1 mM diethylenetriaminepentaacetic acid (DTPA) and 0.02% sodium azide were mixed to a final concentration in a 0.1M phosphate buffer (pH 7.0) 180 占 퐇 was dispensed into each well plate (Nunc, Denmark), and then 20 占 퐇 of each sample (10 mg / ml) was added. At this time, a control group (A) in which glycosylation did not occur without mixing the ribose, a glycosylation control group (B) in which glycosylation occurred in the preparation, and a sample treatment group (C) were prepared.

A sample treatment group (D) without treatment with ribose was also prepared to eliminate the interference of fluorescence by each sample. After completion of the reaction, the reaction was carried out for 7 days at 37 ° C in a constant-temperature and constant-humidity incubator to prevent drying during the reaction. After the reaction was completed, fluorescence was measured at ex = 370 nm / em = 440 nm using a spectrophotometer (VICTOR3, Perkin Elmer, Waltham, MA, USA). Each sample was repeated three times.

The inhibitory activity of the final glycation end product was 100% for the control group (A) without saccharification reaction and 0% for the glycosylation control group (B) in which the glycation reaction occurred. (CD), which excluded the fluorescence of each sample in which no saccharification reaction occurred, was used. As a control, aminoguanidine was used, and samples were measured by adding Comparative Example 1, Example 1, and Example 2. The inhibition rate (%) of the final glycation end product formation was calculated by the following formula, and the results are shown in Table 1 below.

(%) = 100 - [(C-D) / (A-B) x 100]

Name of sample Treatment concentration (w / v%) Production inhibition rate (%) Example 1 0.1 35.4 Example 2 0.1 58.2 Comparative Example 1 0.1 32.9       aminoguanidine 0.1 40.8

As shown in the above Table 1, it was confirmed that colloidal gold-virion extracts extracted with colloidal gold solution had better inhibitory effect on final glycation endproducts than the ordinary bird's nest extracts or colloidal gold aqueous solutions.

Test Example 2: Confirmation of inhibitory effect of inflammatory mediator

RAW 264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After incubation, starvation was maintained for 12 hours, LPS 10 ng / mL and samples were treated and incubated for 24 hours. RNA was extracted from the cultured cells and the inhibition rate of TNF-α expression was confirmed by RT-PCR (reverse transcription polymerase chain reaction). The inhibition rate of TNF-α expression was calculated by the following formula, and the results are shown in Table 2 below. Dexamethasone was used as a control.

TNF-α expression level (%) = (sample TNF-α expression level / control group TNF-α expression level) × 100

Name of sample Treatment concentration (w / v%) TNF-α inhibition rate (%) Example 1 0.1 68.2 Example 2 0.1 79.8 Comparative Example 1 0.1 72.0 Dexamethason 0.1 70.1

As shown in the above Table 2, it was confirmed that colloidal gold-virion extract extracted with colloidal gold solution had better TNF-α expression inhibitory effect than the usual bird's nest extract or colloidal gold aqueous solution at the same concentration treatment.

Test Example 3: Confirmation of increasing effect of moisturizing factor

CCD-986Sk cells were adjusted to 5 × 10 ⁵ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After culturing, the samples were treated and incubated for 24 hours. RNA was extracted from cultured cells and the expression of Hyaluronan Synthase-3 (HAS-3) was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The HAS-3 expression increase rate was calculated by the following equation, and the results are shown in Table 3 below. As a control group, hyaluronic acid was used.

(%) = (Expression amount of sample HAS-3 / expression amount of control HAS-3) × 100

Name of sample Treatment concentration (w / v%) HAS-3 growth rate (%) Example 1 0.1 82.7 Example 2 0.1 92.1 Comparative Example 1 0.1 85.3 Hyaluronic Acid 0.1 83.8

As shown in Table 3 above, it was confirmed that the colloidal gold-virion extract extracted with colloidal gold solution exhibited a better HAS-3 expression enhancement effect than the normal bird's nest extract or colloidal gold aqueous solution at the same concentration treatment.

Examples 3-4: Preparation of lotion containing colloidal gold-virgin extract

Lotion containing colloidal gold-bird's nest extract (Example 2) extracted using a colloidal gold aqueous solution was prepared according to a conventional method with the composition and content shown in Table 4 below.

Comparative Example 2: Production of lotion containing bird's nest extract

Lotions containing the bird's nest extract (Comparative Example 1) were prepared according to a conventional method with the composition and content shown in Table 4 below.

(weight%) Control Example Comparative Example 2 Example 3 Example 4 Comparative Example 1 - 0.5 - - Example 2 - - 0.5 1.0 Wax 1.0 1.0 1.0 1.0 Polysorbate 60 1.5 1.5 1.5 1.5 Sorbitan sesquioleate 0.5 0.5 0.5 0.5 Liquid paraffin 10.0 10.0 10.0 10.0 Sorbitan stearate 0.5 0.5 0.5 0.5 Chin type monostearate
glycerin 0.5 0.5 0.5 0.5 Stearic acid 1.5 1.5 1.5 1.5 Glyceryl stearate /
FAGE-400 stearate 1.0 1.0 1.0 1.0 Propylene glycol 3.0 3.0 3.0 3.0 Carboxy polymer 0.1 0.1 0.1 0.1 Triethanolamine 0.2 0.2 0.2 0.2 antiseptic a very small amount a very small amount a very small amount a very small amount Purified water To 100 To 100 To 100 To 100

Test Example 4: Confirmation of skin soothing effect

In 10 male and 20 female subjects aged between 20 and 30 years without skin allergy symptoms, skin irritation was induced by tape stripping method on the inner part of the lower arm, The degree of stimulation induced by applying a cosmetic composition and a cosmetic composition not containing the cosmetic was relatively evaluated. First, taping was repeated 30 times with a scotch tape on both sides of the underbody to cause skin erythema. After the erythema induction, the cosmetic composition containing the colloidal gold bird's nest extract according to the present invention was applied and the cosmetic composition containing the colloidal gold bird's nest extract according to the present invention was applied. The degree of skin soothing was evaluated 3 hours after application.

≪ Evaluation of degree of skin irritation (erythema) >

0 (no response) 1 (very mild erythema) 2 (faint or mild erythema)

3 (clear erythema but weak erythema) 4 (pronounced erythema) 5 (severe erythema)

Name of sample Degree of skin irritation Left Right Example 3 One 0 Example 4 0 0 Comparative Example 2 2 One Control Example 2 2

As shown in Table 5, the lotion compositions of Examples 3 to 4 containing the colloidal gold-bird's nest extract according to the present invention were superior in the skin irritation mitigation effect as compared with the control and comparison examples not containing the extract. Among them, the lotion formulation of Example 4 showed the best skin irritation mitigation effect.

Claims (10)

A cosmetic composition comprising a bird's nest extract extracted with colloidal gold as a solvent as an active ingredient.       The cosmetic composition according to claim 1, wherein the colloidal gold solution contains gold particles at a concentration of 1 to 10000 ppm. The method of claim 1, wherein the colloidal gold solution comprises: A) dissolving gold salts in a solvent to prepare a gold solution; B) reducing the reducing agent by adding a reducing agent; C) cleaning and drying to produce gold particles; And D) dissolving the gold particles in a solvent. 4. The method of claim 3, wherein the gold salt is selected from the group consisting of gold chloride, gold bromide, gold sulfide, chloroauric acid, gold hydroxide, Wherein the cosmetic composition is at least one selected from the group consisting of Chlorotriethyl phosphine gold and Chlorotriphenylphosphine gold. The method of claim 3, wherein the reducing agent is selected from the group consisting of ethylene oxide, sodium borohydride, sodium citrate, thiocyanate, and ascorbic acid. Wherein the cosmetic composition is at least one selected from the group consisting of: The cosmetic composition according to claim 1, wherein the cosmetic composition has an anti-glycation effect. [Claim 3] The cosmetic composition according to claim 1, wherein the cosmetic composition has an activity of suppressing the inflammatory mediator expression of skin. [Claim 7] The cosmetic composition according to claim 1, wherein the cosmetic composition increases the moisturizing power of the skin through increase in the expression of a moisturizing factor. The cosmetic composition according to claim 1, wherein the cosmetic composition has a skin irritation-mitigating effect. A) adding 5 to 10 times by weight colloidal gold solution to the chopped bird's nest after drying;
B) extracting at a low temperature of 4 to 50 캜 for 3 days; And
C) a step of adding a polyol to remove impurities, and aging the mixture by immersing the mixture at a low temperature of 4 to 50 DEG C for about 3 to 4 days, thereby producing a colloidal gold bird's nest extract.