KR100989944B1 - A mixed herb medicine extracts having antioxidant whitening activity - Google Patents

Abstract

PURPOSE: An extract with antioxidation and whitening using natural herb mixture is provided to ensure antoxidation activity by DPPH radical removal ability and skin whitening by tyrosinase suppression. CONSTITUTION: A natural herb mixture extract with antioxidation and skin whitening contains extract of 20-40 weight% of Witch Hazel(Hamamelis Virginiana), 20-40 weight% of Eucalyptus Globulus, 5-15 weight% of Melissa Officinalis, 15-20 weight% of rosehip(Rosa canina L.), 2-8 weight% of Mentha piperita L., and 2-8 weight% of Origanum vulgare. The extract is isolated by ultrasonic extraction. A pharmaceutical composition for oxidative whitening contains the natural herb mixture extract as an active ingredient. A cosmetic composition for oxidative whitening contains the natural herb mixture extract as an active ingredient.

Description

A mixed herb medicine extracts having antioxidant whitening activity

The present invention relates to a herbal blend extract having an antioxidant whitening activity, more specifically, an extract having an antioxidant whitening activity prepared using a herbal mixture of witch hazel, eucalyptus, lemon balm, rosehip, thyme and oregano, and an active ingredient It relates to a composition for antioxidant whitening to contain.

Witch Hazel (Hamamelis Virginiana) is a capsule-like fruit that is hard, covered with black layers, and the seeds are white, oily and edible, and are widely distributed to Ontario in the west, Texas and Florida in the south. It is distributed. The land grows on ordinary land and grows well even if it is not good soil. Many branches are stretched and the light brown fruit is brown with 1/2 inch round capsule shape. Beta-Yonon, Gallic Acid, Isokercitrin, Camperol, Leucodelpinidine, Mycetin, Phenol, Quercetin, Quercetin, Saponin, Tannin, etc. It is used as a sedative, and it is effective when intestinal bleeding, gum bleeding, hemorrhoids and venous abnormalities, and it is a cut, burn, diarrhea, dysentery, bleeding, insect bites, excessive menstruation, tuberculosis, cancer, muscle pain, burning, bruising Use when heard. In addition, the skin sedation and skin protection effect is excellent, it is effective for allergic skin or inflammatory skin, strong astringent action, helps to stabilize the skin, excellent moisturizing effect.

In connection with witch hazel, a cosmetic composition prepared to have an excellent oxygen purification cleansing effect by containing witch hazel as an active ingredient in Korean Patent Application No. 10-2005-0020479 “Oxygen Cleansing Cleansing Composition” is disclosed. [0002] A mask pack containing witch hazel is disclosed in Utility Model No. 20-0277185, "Mask Pack."

Eucalyptus (Eucalyptus Globulus leaf extract) is mostly a very large tree belonging to the Platinum family, and its origin is Australia, New Zealand, Tasmania and neighboring islands. There are over 500 species, and in Australia eucalypti is known primarily as gum or stringy-bark trees. Many species are shade trees and are widely grown in many areas. The leaves are cortical, hanging obliquely or vertically. The petals stick together to form a CAP when the flower buds. Fruits are surrounded by wooden cupped jaws and contain many fine seeds. The largest fruits are 5-6 cm in diameter, known as mottlecah, silver-leaf and eucalyptus. Eucalypti grows fast, and many species are tall. The big sword tree has a height of 90m and its circumference is 7.5m. Many species, especially the leaf gland of E. salicifolia and E. globulus, contain volatile and aromatic oils, known as eucalyptus oil. The main ingredient is 3% essential oil, 80% is cineol, pinene, terpineol, etc., Tanizil, carbon, chlorogenic acid, ellagic acid, hyperside, parasimine, protocatechuic acid, Quercetin, quercitrin, rutin and the like.

Regarding eucalyptus, Korean Patent No. 10-0490982 “Antimicrobial composition comprising eucalyptus essential oil as an active ingredient and an antimicrobial agent comprising the composition”, Korean Patent Application No. 10-2003-0000644 “Including eucalyptus essential oil as an active ingredient To insecticide ”, Republic of Korea Patent Application No. 10-2009-7004185" Eucalyptus extract, its preparation and therapeutic uses "and the like are disclosed.

Lemon balm (Melissa Officinalis leaf extract) is native to the Mediterranean coast and is a wheat plant native to eastern Mediterranean, West Asia, Black Sea coast and Central European marsh. The stem is straight and the branches spread lushly everywhere. The leaves are opposite, long and round, and the ends are pointed. The sowing season is from April to June and from September to October, and it grows in the sunny and well-cultivated fertile soil. From mid-June to early September, white and small flowers grow on the petioles, 3 ~ 7 small, growing up from the bottom of the stem. Breed by seeding, folding, and giving up. Blooming season is mainly from July to August. It has a lemon-like scent that calms feelings and lowers heart rate by lowering heart rate. Efficacy in detoxification and fat loss, soothing, healthy, tonic, nerve uplift, etc. It is also used for food.

Also known as Melissa, lemon balm is an important herb that has been cultivated since ancient Greece because the fresh, juicy lemon scent attracts bees. Lemon balm loves bright halftones and grows well in organic, fertilized soils with adequate moisture. It is resistant to both low and high temperatures, and grows well even in high humidity, but if it dries a little in summer, it leaves no shine and discoloration occurs. After planting, it is effective to squeeze it once or twice to give a sense of volume, and in winter, the ground will dry out, so it is better to cut the bottom and winter. When planting pots, depending on whether they grow twice a year, change into large pots or give up, and fertilize once a month. The lemon balm, which spreads like mint, can be harvested at any time since the original stem comes out when the original stem is cut. When drying and preserving leaves, it is better to do it before flowering. Cut 5-6 stem stems with buds, tie them with strings, hang them upside down and dry them in the shade. In particular, when drying at high temperatures above 60 degrees, the fragrance will fall out and the leaves will be discolored.

Lemon balm is effective in reducing detoxification, diarrhea, and virus, and is effective for smallpox. Taking leaves with potassium nitrate is good for detoxification and abdominal pain, and taking it with salt is also effective for ulcers. It is effective in suppressing menstrual pain and promoting menstruation. Rinse with lemon balm oil to help prevent hair loss, and use it as a bath to help your body recover and warm up your skin.

Regarding lemon balm, Korean Patent Application No. 10-2000-0007187 “Massage Salt Using Lemon Balm”, Republic of Korea Patent No. 10-0899889 “Method of manufacturing leaching tea and liquid extract tea containing lemon and lemon balm and Leaching tea and liquid extraction tea manufactured therefrom.

Rosehip ( Rosa canina L. ) is a fruit of wild rose called 'Rosa Mosqueta' which grows in the Andes of Chile, 2000-3000m above sea level. In the past when fruit was not common as it is today, it was used as a dessert, and it was a rich source of vitamin C, especially effective for scurvy. Rosehip is widely evaluated in the United States and Europe, such as rosehip-based cosmetics and natural vitamins are commercially popular, but is currently recognized as an unfamiliar herb in Korea.

The vitamin content of rosehip is 20 times that of orange and 60 times that of lemon. It acts as a diuretic with vitamin A, which promotes the growth and development of children. Relieves eye fatigue, constipation, and cramps, and is good for nutrition when eating hot, cold, and during pregnancy. It prevents the skin of people who drink alcohol and tobacco from roughening, it is also good for beauty effects. Pectin in rosehips can be used as a light laxative and helps regular bowel movements. Rosehip oil is known to be effective on the cuts such as skin wounds and burns caused by trauma and has an excellent regeneration effect.

Rosehip has a high content of vitamin C and carotene and has been used to treat and prevent scurvy and vitamin C deficiency. Rosehips also have very good antioxidant activity. This is a rich source of vitamin C. Rosehip contains 60 times more vitamin C than lemon, and tea can help prevent colds and reduce chills. In Germany, rose hips were used for the production of bile, to treat abdominal pain and edema of the urinary system, and to strengthen the kidneys. Rosehip also acts as a diuretic and astringent, strengthening bones and joints. Pectin, contained in rosehips, can be used as a light laxative and helps with regular bowel movements. Rosehip oil is known to be effective in the cuts such as skin wounds and burns caused by trauma and has a very good regenerating effect.

In connection with rosehips, Korean Patent Application No. 10-2009-7002869 discloses a composition for the treatment of inflammatory diseases comprising rosehips and other active agents.

Thyme ( Mentha piperita L. ) is a hybrid of the watermint (Mentha aguatica) and the spearmint (Mentha spicata), which is named peppermint because its aroma resembles the peppery's tangy nature. Although it is native to Europe, it also grows in Europe, the United States, Japan, and China. The world's major supplier is the U.S., but the UK is considered the best. Mint is about 20 species, including Eau de Colon Colon, Apple Mint, Penny Royal Mint, Peppermint, Spearmint, and Black Peppermint. One of the strongest herbs ever grown, the soil can be anywhere but prefers a slightly damp halftone rather than sunny. It grows wild in fields or wetlands and has many varieties due to its good hybridization. The stem is square, the leaves are sublime, and there are crabs. Abandonment spreads to crawling ground and lanna of root. The flowers are white or purple, closely attached with water inflorescences in July-September, and bloomed the next year after planting. Leaves have a number of oil spots that give off aromas on the back, and the main component of essential oils is menthol. Cold resistance is strong but weak at high temperature and drying.

Peppermint is especially used for toothpaste among mints because it has high bactericidal and antiparasitic effects and is effective for removing bad breath. Grinding leaves as a poultice is effective for skin irritation, bruises, itching, and when placed in the bathroom, peppermint fragrance lasts long. Gastrointestinal disease, headache, cholera, diarrhea, hysteria, neuralgia, rheumatism, toothache, acid fever, colic, etc. It is used as an anti-inflammatory, analgesic, antiperspirant, and antiseptic agent. It is said that he does not have a cold in winter. Increases stomach acidity, helps digestion, weakly mucous membranes and digestive tract, chills, abdominal pain, headache, heart disease, motion sickness, loss of appetite, cramps

Origanum vulgare is native to Southern Europe and West Asia, and it is a perennial herb of Lamiaceae. The flowers are purple, red, and vital. It is also known as flower mint, and it has a scent like mint. The height is 30 ~ 60㎝, the leaves are about 1.5㎝ long and have a good aroma and bitter taste. Oregano, nicknamed 'Wild marjoram', is a herb that stands up to pests and cold as its name suggests, and the strength of wildflowers stands out in Europe and Southwest Asia. It is a perennial plant of Lamiaceae and is a viable species among many species of marjoram. It is the same genus as sweet marjoram, but its smell and taste are more excellent.

Oregano grows as if the stem is 30 to 90 centimeters in length, spreading horizontally to the ground. The flowers are 4mm in diameter, purple-pink in lip, and form a watery inflorescence at the end of the stem from July to October. It is the only herb used in marjoram robes of many cultivars. It has been used as an ornamental herb since ancient times. Stems, leaves, and flowers are used for cooking, bathing, potpourri, dyeing, and ornaments. Harvest and dry the flowers at the time of flowering and preserve the dried leaves with spices. Its unique aroma and spicy taste go well with tomatoes, making it an indispensable spice for Italian dishes, especially pizzas.

Oregano leachate, used as an herb from ancient Greece, has a tonic, diuretic, dry stomach, appetite enhancement, soothing and disinfecting effect.It is used to boil tea, potpourri and bath. Irritating oregano tea is especially liked by men, which helps to get rid of chills, help digestion, increase appetite, and also help with stomach sickness. It also has antiseptic and detoxification effects, so it is famous as an antidote when bitten by snakes or scorpions, and it can also prevent ants from entering the house. The fragrance ingredients from the leaves also have a tonic effect, most of which are used as food flavors rather than medicinal. Inhaling the oil in a towel can help treat neurological headaches or insomnia, and it can also be used as a dry flower, with dry leaves and flowers in a pocket that can be used for hot compresses in patients with rheumatism.

Regarding oregano, Korean Patent Application No. 10-2003-0041938 discloses oregano extract for protecting crops from phytopathogenic fungi, and Korean Patent Application No. 10-2008-0027185 discloses oregano fermentation and maintenance compositions.

On the other hand, a common method of extracting the active ingredient from the copper and plants is to use an organic solvent such as water, methanol, ethanol, hexane, etc. The extraction method using the organic solvent is easy to apply, while selecting the appropriate solvent, remaining of the organic solvent It is difficult to remove solvents, environmental pollution, low extraction yield, and usually requires a long extraction time of about 24 to 48 hours. However, for commercial use, it is necessary to shorten the stability and extraction time of the solvent, to acquire the environmental pollution and the ingredients to be extracted and to have the activity, but there is no clear method for this.

In addition, the hydrothermal extraction method has low extraction efficiency, high energy consumption, and has the disadvantages of destruction and loss of many useful components due to heat, protein variation, and heat instability.

As an alternative to the organic solvent extraction method or the hot water extraction method, the ultrasonic extraction method is applied to obtain good efficiency for fast reaction time in the grinding, mixing, and extraction processes. Many bioactives have the advantage of being safe and effective. In the ultrasonic extraction method, ultrasonic waves are sounds (sound waves) and sound of high frequency that cannot be heard by the human ear, where low temperature refers to 60 ° C. or less.

Regarding the ultrasonic extraction method, Republic of Korea Patent No. 10-0823611 "Extraction method of isoflavones and extraction of soy isoflavones from soybeans using ultrasonic waves", Republic of Korea Patent No. 10-0886201 "Jecheonsan Hwanggi manufactured by ultrasonic extraction Cosmetic composition for skin moisturizing containing the extract ”, Republic of Korea Patent No. 10-0779855" A method of extracting buckwheat sprout using ultrasonic waves "and the like are disclosed.

Plant mixtures are generally designed to avoid hot or cryogenic levels of extraction temperature to selectively remove unwanted components, selectively enhance active ingredients, and overcome the disadvantages of inadequate selectivity of solvents by ultrasonic extraction. .

The extract by the ultrasonic extraction method has an excellent ultraviolet absorption effect compared to the extract extracted by the hot water extraction method and the solvent extraction method, has a high non-absorbance, solubility and affinity for various bases and additives for cosmetics, organic solvents, etc. And stability is good, it is possible to provide a cosmetic composition excellent in the effect of protecting the skin from various stresses moist and clean.

In the preparation of plant extracts, the advantage of the ultrasonic extraction method is that the intensity of the ultrasonic energy change can be adjusted according to the raw material to be extracted, and accordingly the extraction efficiency is very high depending on the raw material, and the modification of the active ingredient exhibiting pharmacological activity and It minimizes the possibility of destruction and shortens the extraction time because the production speed is faster than the conventional hydrothermal extraction method, which results in low cost and low energy.

In the present invention, the herbal ingredients having antioxidant whitening activity are searched for witch hazel, eucalyptus, lemon balm, rosehip, thyme and oregano as described above, and the herbal extracts are prepared by applying an ultrasonic extraction method to the selected herbal mixtures. It was confirmed that they have superior activity than the extracts prepared by the conventional extraction method and led to the present invention.

Accordingly, an object of the present invention is to provide a herbal mixture extract excellent in antioxidant and whitening activity prepared by ultrasonic extraction using a herbal mixture of witch hazel, eucalyptus, lemon balm, rosehip, thyme and oregano.

Another object of the present invention is to provide an antioxidant whitening composition containing the herbal extract as an active ingredient.

The purpose of the present invention as described above is to prepare a herbal mixture extract by ultrasonic extraction method using a herbal mixture of witch hazel, eucalyptus, lemon balm, rosehip, thyme and oregano, DPPH radical scavenging ability and tyrosinase inhibition of the herbal mixture extract It was achieved by confirming that it had good antioxidant and whitening activity by measuring activity.

The present invention provides a herbal mixed extract having antioxidant whitening activity consisting of witch hazel, eucalyptus, lemon balm, rosehip, thyme and oregano.

The herbal mixed extract according to the present invention is 20 to 40% by weight of witch hazel, 20 to 40% by weight of eucalyptus, 5 to 15% by weight of lemon balm, 15 to 25% by weight of rose hips, 2 to 8% by weight and oregano 2 to 8 It is composed of 30% by weight of witch hazel, 30% by weight of eucalyptus, 10% by weight of lemon balm, 20% by weight of rose hips, 5% by weight of thyme and 5% by weight of oregano.

The herbal mixture extract according to the present invention comprises 10 times to one or more solvents selected from the group consisting of distilled water, ethanol, methanol, butanol, acetone, ethyl acetate, hexane, propanol, hydrous butylene glycol, and hydrous propylene glycol as extraction solvents. It may be prepared by a method of extracting by heating at a volume of 20 times, by heating at 50 to 100 ℃ for 4 to 20 hours in a state that the cooling capacitor is installed to prevent the solvent from evaporating.

In addition, the herbal mixed extract according to the present invention comprises a distilled water, ethanol, methanol, butanol, acetone, ethyl acetate, hexane, propanol, hydrous butylene glycol, hydrous propylene glycol as an extraction solvent to the sample 10 After adding to a volume of 20 times to 20 times, it can be prepared using an ultrasonic extraction method of 1 hour to 10 hours, preferably 1 hour to 4 hours, wherein in the general hydrothermal to solvent extraction method 1 By applying a long-term continuous or more than two times of intermittent rotation can maximize the extraction efficiency of the active ingredient.

In the present invention, the term "herbal herbal extract" or "herbal extract" means an extract prepared by extracting the herbal mixture consisting of the eucalyptus, lemon balm, rosehip, thyme and oregano by the above method.

The herbal mixture extract prepared by using the herbal mixture of witch hazel, eucalyptus, lemon balm, rosehip, thyme and oregano according to the present invention has antioxidant activity through excellent DPPH radical scavenging activity, and whitening effect through tyrosinase inhibitory activity. great.

In another aspect, the present invention provides an antioxidant whitening pharmaceutical composition containing the herbal extract as an active ingredient.

In the present invention, the term "active ingredient" refers to a substance or a group of substances (a medicinal agent whose pharmacologically active ingredient is not known, etc.) which is expected to express the efficacy or effect of the medicine directly or indirectly by inherent pharmacological action. It means containing a main component).

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Dosage forms of the pharmaceutical compositions of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable collection.

In another aspect, the present invention provides a cosmetic composition for improving antioxidant whitening containing the herbal extract as an active ingredient.

Cosmetic compositions of the present invention further comprise physiologically acceptable media such as fatty substances, preservatives, vitamins, gelling agents, flavoring agents, surfactants, water, antioxidants, fillers, wetting agents, masking agents and mixtures thereof. can do.

The cosmetic composition according to the present invention may be prepared and used in the formulation of cosmetics, cosmetics, makeup products, essences, creams, lotions, packs, foundations, makeup bases and the like. It can be applied in various forms such as liquid, cream, paste, solid, and can be prepared by a conventional cosmetic preparation method.

The cosmetic composition according to the present invention is added in an amount of 1 to 10% by weight, preferably 1 to 5% by weight, as a dry weight of the herbal mixture extract.

The herbal mixture extract prepared using a herbal mixture of witch hazel, eucalyptus, lemon balm, rosehip, thyme and oregano according to the present invention has an antioxidant activity through excellent DPPH radical scavenging activity and a whitening effect through tyrosinase inhibitory activity. This has an excellent effect.

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited to these examples.

Example 1 Preparation of Extracts of Herbal Medicines Using Ultrasonic Extraction

In this example, a herbal mixture consisting of witch hazel, eucalyptus, lemon balm, rosehip, thyme and oregano was extracted by ultrasonic extraction. 30 g of witch hazel, 30 g of eucalyptus, 10 g of lemon balm, 20 g of rose hips, 5 g of thyme and 5 g of oregano are added thereto, and 10 times of distilled water is added thereto, followed by mounting a cooling condenser to prevent evaporation of the solvent from 1 hour to 1 hour. The herbal mixture extract was prepared using an ultrasonic extraction method for 4 hours.

Experimental Example 1 Measurement of Antioxidant Activity by DPPH Scavenging Activity

In this experimental example, the antioxidant activity of the herbal mixture extract prepared in Example 1 was measured.

The process of skin aging with free radicals includes lipid peroxidation, protein oxidation, DNA oxidation, melanin production, cleavage of connective tissue and abnormal crosslinks. These reactions are almost always radical reactions, which include an automatic oxidation process. Antioxidants are responsible for blocking the automatic oxidation in vivo. The greater the ability to give electrons, that is, the greater the reducing power, the stronger the antioxidant. One reagent that can measure the reducing power of antioxidants is 1,1-diphenyl-2picrylhydrazyl radical (DPPH). DPPH exists as a radical of a stabilized structure of the nitrogen center in the compound.

In order to measure the scavenging ability of the stable free radical 1,1-diphenyl-2-picryl-hydrazyl (DPPH), the herbal mixture extract was mixed with 100 μL of DPPH radical in methanol solution (0.2 mM). After incubating for 20 minutes at room temperature, the absorbance at 517 nm was measured using a microplate reader, and the results are shown in FIG. 1.

Experimental Example 2 Measurement of Tyrosinase Inhibition Activity

In this experimental example, tyrosinase inhibitory activity of the herbal mixture extract prepared in Example 1 was measured.

Excessive melanin synthesis forms blemishes, freckles, and black mushrooms in the human body, promotes skin aging, and is involved in skin cancer. This melanin begins to be synthesized by the biosynthesis of an enzyme called tyrosinase in ribosomes, which are intracellular organelles, and is synthesized in several steps from tyrosine, an amino acid, by the action of this enzyme, and deposited on the surface of the black vesicles called melanosite. The melanin granules are produced. In order to prevent the deposition of melanin pigments, various methods have been used in the development of skin whitening raw materials, and in vitro tyrosinase activity inhibition experiments are most frequently used.

Test compound solution was prepared by mixing the herbal mixture extract with DMSO and 0.1 M phosphate buffer (pH 7.0). The test tube was then filled with 80 μL of 0.1 M phosphate buffer (pH 7.0), 40 μL of test compound solution and 40 μL of mushroom tyrosinase (1,500-2,000 U / μL). Then 40 μL of 2.5 mM L-dopa was added. On the other hand, the same control solution containing no test compound was prepared. UV absorbance at 475 nm was measured using a microplate reader, and the inhibition rate was calculated using the following equation, and the results are shown in FIG. 2.

% Inhibition = [(Ac-As) / Ac] X 100

Where Ac is the absorbency of the control and As is the absorbency of the sample.

1 is a graph showing the antioxidant activity of the herbal extracts of the present invention.

Figure 2 is a graph showing the tyrosinase inhibitory activity of the herbal extracts of the present invention.

Claims (5)

Herbal mixed extract with antioxidant whitening activity consisting of witch hazel, eucalyptus, lemon balm, rosehip, thyme and oregano. According to claim 1, wherein the herbal extract is 20 to 40% by weight of witch hazel, 20 to 40% by weight of eucalyptus, 5 to 15% by weight of lemon balm, 15 to 25% by weight of rose hips, 2 to 8% by weight and oregano 2 Herbal mixed extract, characterized in that consisting of to 8% by weight. According to claim 1, wherein the herbal mixture extract is a herbal mixture extract, characterized in that extracted by ultrasonic extraction. Antioxidant whitening pharmaceutical composition containing the herbal extract according to claim 1 as an active ingredient. Antioxidant whitening cosmetic composition comprising the herbal extract according to claim 1 as an active ingredient.

Images