KR100963774B1 - Plant disease control agent and plant growth promoter using Bacillus megaterium VINCC 251 having the effect of controlling plant diseases and promoting plant growth and the strain - Google Patents

Abstract

The present invention is directed to Bacillus megaterium KNUC251 ( Bacillus) which activates induction resistance-defense mechanisms of plants against plant pathogens. megaterium KNUC251) strain, and more specifically, the Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251) or a plant disease control agent and plant growth promoter containing the culture filtrate of the strain. In addition, the strain relates to a culture method of the strain of Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251), characterized in that incubated for 24 hours at 37 ℃ on TSA medium.

The plant disease control agent and the plant growth promoter as described above have the effect of promoting the control effect and the growth of the plant against plant diseases occurring in the dicotyledonous plants, the effect is an effect that appears independently. In addition, the dicotyledonous plant is characterized in that it is selected from the group consisting of pepper, tobacco, tomato. In addition, the plant disease is characterized in that the bacterium of bacterial spot pattern or tobacco purine disease.

Bacillus megaterium, plant disease control, induction resistance-activation of defense mechanism

Description

Bacillus megaterium KNUC251 for controlling plant diseases and promoting plant growth, and plant disease control and plant growth promoters using the strains (Bacillus megaterium KNUC251 for controlling plant disease and accelerating plant growth, and plant disease controlling agent and plant growth accelerant using the same}

The present invention is directed to Bacillus megaterium KNUC251 ( Bacillus) which activates induction resistance-defense mechanisms of plants against plant pathogens. megaterium KNUC251) strain, and more specifically, the Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251) or a plant disease control agent and plant growth promoter containing the culture filtrate of the strain. In addition, the strain relates to a culture method of the strain of Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251), characterized in that incubated for 24 hours at 37 ℃ on TSA medium.

In general, in order to control plant diseases caused by plants in order to increase production in agriculture, countries around the world have manufactured and used pesticides based on organic compounds. The use of such chemical pesticides has caused harmful effects such as environmental pollution, human toxicity caused by residual pesticides, and the appearance of pathogens resistant to pesticides. Therefore, research on environmentally friendly plant disease control (biological control) by microorganisms such as bacteria and fungi has begun as a plant disease control technology to replace organic synthetic pesticides.

As one of the above studies, ISR (Induced Systemic Resistance) of a plant was studied. The ISR (Induced Systemic Resistance) is a technology that can control disease by operating a self-protection system owned by a plant. Once resistance to plants is induced by the induced systemic resistance (ISR), various diseases such as mold, bacteria, and viruses can be controlled in combination.

As one of the studies of the induced systemic resistance (ISR), the release material of the root region of the Festuca ovina L (Sheep fescue) mutant was analyzed. As a result of the analysis, m-tyrosine amino acids were found to be herbicides in which plant mutations inhibit the growth of surrounding weeds. The m-tyrosine is similar to the structure of para-tyrosine, which is one of the amino acids forming a protein. During the experiment, m-tyrosine did not inhibit fungi, mammals, and bacteria, but produced toxic substances only on poisonous plants, thus inhibiting growth and removing them.

Further research has found that the Sheep fescue mutant releases m-tyrosine as well as a common amino acid, phenylalanine (an amino acid that plants synthesize cell walls and lignin). When plant growth exposed to m-tyrosine is inhibited or dying, spraying phenylalanine on the root site restores vitality. That is, the plant mutant releases a large amount of phenylalanine while releasing m-tyrosine (meta-tyrosine) and decodes it by itself. In addition, a mechanism for inhibiting plant growth by m-tyrosine has been proposed.

The phenylalanine (phenylalanine), m-tyrosine (meta-tyrosine), p-tyrosine (para-tyrosine) is similar in structure, based on this property m-tyrosine (meta-tyrosine) is a high concentration of phenylalanine released by other plant roots It replaced (phenylalanine) and inhibited plant growth by interfering with the normal phenylalanine synthesis function.

The study of m-tyrosine, which inhibits plant growth, further understands the effect of allelopathy (the role of plants affecting other plants through the release of chemicals) and is also effective. Natural and natural herbicides (m-tyrosine) were readily soluble in water and not used directly in herbicides, providing the basis for research and development (KISTI GTB 2007.11.16).

However, the eco-friendly biological control technology used microorganisms directly exhibiting antimicrobial activity against plant pathogens, but its commercial range is limited, and its production varies considerably according to environmental conditions, causing many problems in use. In addition, the Bacillus megaterium KNUC251 ( Bacillus megaterium There was no method of controlling plant diseases using KNUC251) strain.

The present invention has been made in order to solve the above problems, the present invention is to increase the induction resistance of plants and effective growth promotion Bacillus megaterium KNUC251 ( Bacillus megaterium It is an object of the present invention to provide a plant disease control agent and a plant growth promoter comprising the strain, the culture method of the strain, the strain or the culture filtrate of the strain.

Bacillus megaterium KNUC251 according to the present invention for achieving the above object ( Bacillus megaterium KNUC251) strain is characterized by activating the plant's induced resistance-defense mechanism against plant pathogens.

Preferably, the plant disease control agent according to the present invention for achieving the above object Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251) or a culture filtrate of the strain is characterized in that it comprises a.

Preferably, the plant disease is a plant disease occurring in a dicotyledonous plant, the dicotyledonous plant is characterized in that selected from the group consisting of pepper, tobacco, tomato.

More preferably, the plant disease is characterized in that the bacterial spot pattern disease of tobacco or tobacco chamomile.

In addition, the plant growth promoter according to the present invention for achieving the above object is the Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251) or a culture filtrate of the strain is characterized in that it contains.

Preferably, the plant growth promoter is to promote the growth of the dicotyledonous plant, the dicotyledonous plant is characterized in that selected from the group consisting of pepper, tobacco, tomato.

Preferably, Bacillus megaterium KNUC251 described above ( Bacillus megaterium The strain of KNUC251) is characterized in that it is incubated for 12 hours to 24 hours at 30 ℃ to 60 ℃ TSA medium.

More preferably, the above-described strain of Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251) is characterized in that it is incubated for 12 to 24 hours at 37 ℃ on the TSA medium.

As described above, Bacillus megaterium KNUC251 ( Bacillus megaterium) to activate the induction resistance-defense mechanism of the plant against the plant pathogen according to the present invention as described above. According to the KNUC251 strain, the strain uses the induced systemic resistance (ISR) of the plant, which has the effect of preventing human toxicity due to environmental pollution and residual pesticides, which have been shown as side effects of pesticides prepared based on organic compounds. have. In addition, since the organic synthetic pesticides can prevent harmful effects such as the appearance of pathogens resistant to pesticides, it is eco-friendly and can increase the yield of plants.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the accompanying drawings, Figure 1 is a detailed view showing the 16S rDNA sequence of Bacillus megaterium KNUC251 strain (KCTC 11177BP), Figure 2 is a bacillus megaterium KNUC251 strain (KCTC 11177BP) plant plant inhibitory effect and growth promoting effect This is a plan view briefly showing the germination of tobacco seeds on the medium to find out.

And, Figure 3 is a graph showing the results of evaluating the control effect of bacterial spot pattern disease of pepper by Bacillus megaterium KNUC251 strain (KCTC 11177BP) at the level of symptoms, Figure 4 is Bacillus megaterium KNUC251 (KCTC 11177BP) strain It is a detailed diagram showing the control effect of bacterial spot pattern disease of pepper by a photograph, Figure 5 is a graph showing the growth promoting effect of pepper by the Bacillus megaterium KNUC251 strain (KCTC 11177BP) by measuring the dry weight.

In addition, Figure 6 is a graph showing the results of evaluating the control effect of tobacco purpura caused by Bacillus megaterium KNUC251 strain in terms of the number of leaves per cigarette individual A is the secondary metabolism produced by the Bacillus megaterium KNUC251 strain (KCTC 11177BP) This is a graph showing the control effect of tobacco softening disease by the product (material spreading in the liquid phase in the medium), B is a graph showing the control effect of tobacco softening disease by the volatile substance produced by Bacillus megaterium KNUC251 strain (KCTC 11177BP) to be.

In addition, Figure 7 is a detailed view showing the control effect of tobacco purine by Bacillus megaterium KNUC251 strain (KCTC 11177BP) and Figure 8 shows the growth promoting effect of tobacco by Bacillus megaterium KNUC251 strain (KCTC 11177BP) It is the detailed figure shown in the photograph.

The present invention is a bacillus megaterium KNUC251 ( Bacillus megaterium) which promotes the growth of plants with plant disease control effect by activating the induction resistance-defense mechanism of plants against plant pathogens KNUC251) strain. In addition, the present invention relates to a cultivation method of the strain and a plant disease control agent and a plant growth promoter containing the strain. Induced resistance-defense mechanism of the plant is called ISR (Induced Systemic Resistance). The induced systemic resistance (ISR) is to maintain the resistance to the disease by activating the resistance reaction that the plant itself has. The plant overcomes various diseases such as fungi, bacteria, viruses, etc., which have been introduced by inducing a system of self-defense (Induced Systemic Resistance). Bacillus megaterium KNUC251 strain of the present invention ( Bacillus megaterium KNUC251) enables the plant's own system of defense, ISR (Induced Systemic Resistance).

Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251) belongs to Bacillus sp. Among bacteria, is Gram-positive bacteria and forms thick-film spores. Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251) is screened through spore-forming bacteria screening process. Bacillus megaterium KNUC251 ( Bacillus megaterium It is preferable to select spore forming bacteria in KNUC251) strain. This is because the spore-forming bacteria have a high survival rate when treated in soil and can be coated on seeds. The strain is incubated for 12 hours to 24 hours at 30 ℃ to 60 ℃ on the TSA medium. When the temperature is 30 ℃ or less, the growth of the strain does not occur, but when the temperature is 60 ℃ or more, the strain is killed, it is preferable that the culture is carried out within the above temperature range. Most preferably the strain is incubated for 12 to 24 hours at 37 ℃ on the TSA medium. The cultivation increases the number of strains.

In addition, the plant disease control agent according to the present invention, the Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251) or the culture filtrate of the strain. The plant disease is a plant disease occurring in a dicotyledonous plant, and the dicotyledonous plant is selected from the group consisting of pepper, tobacco and tomato.

The dicotyledonous plant is called dicotyledonous plant in other words. The dicotyledonous plant belongs to a pizza plant, and the dicotyledonous means that there are two cotyledons. In addition to the cotyledon, the dicotyledonous plant has an open vascular bundle, and a predetermined number is formed in a ring-shaped or tubular shape, and there is always a formation layer. In addition, the leaves are single leaves, broad, divided into wing-shaped or palm-shaped, sometimes double-leaved to form a net. Moreover, the part which comprises the flower of said dicotyledonous plant is a multiple of 5 or 4, and the seed | pear pear is large.

The strain of the present invention has a control effect against bacterial spot pattern disease of tobacco or tobacco chamomile disease among many plant diseases. Bacterial spot of the pepper (징후 性 斑點 病; Bacterial spot) is a symptom of the disease is characterized by the appearance of stains on the leaves, petiole, stem, fruit and fruit of the pepper. Bacterial spot of the pepper (고추 性 斑點 病; Bacterial spot) initially shows a dark brown round or irregular small spot pattern on the leaves of the pepper and is accompanied by a pale yellow band (halo) around the dark brown spot pattern.

Bacterial spot disease of the red pepper (細菌 性 고추; Bacterial spot) of the disease progresses the center of the lesion is white, the edge of the alum is brown or black brown, several lesions are combined to become a large lesion. Bacterial spot of the pepper is severe (細菌 性 斑點 病; Bacterial spot) is severe, the leaves of the pepper is dried, and the leaves of the pepper is dark brown lesions are formed. In addition, a small brown pattern of dark brown immersion is formed on the stem of the red pepper, and the center of the lesion is convex later.

Bacterial spot pathogens of the red pepper are Xanthomonas axonopodis pv. Bessictoria ( Xanthomonas axonopodis pv. vesicatoria, a gram-negative mononuclear bacillus, aerobic, monopolar, and motile. Xanthomonas Campestris pv. The size of the cells of Xanthomonas campestris pv.vesicatorina is 0.7-1.0 × 2.0-2.4 μm, and the pathogens form yellow colonies in solid medium and grow slowly. The growth temperature of the pathogen is 24 to 30 ℃. It is known that passage of most pathogens in artificial media for a long time weakens the pathogenicity, but the pathogen maintains relatively strong pathogenicity even if it is passaged more than 15 times.

The pathogens overwinter in the remnants of diseased plants and are capable of soil transmission, but seed infections caused by contamination and infection of the epidermis are most common. In addition, pathogens are scattered and moved by rainwater from plant tissues and invade plants through pores and wounds. The pathogens are likely to occur in cool, high humidity environmental conditions.

In addition, the cigarette sclerosis is caused by the pathogen Pectobacterium carotovorum subsp. Carotovora or Erwinia carotovora subsp. Carotovora. do. The pathogen belongs to monophyte, has a size of 1.5 to 3.0 x 0.6 to 0.9 mu m, and has 2 to 10 hairs. The growth temperature of the pathogen is 32-35 ° C., maximum 40 ° C., minimum 0-9 ° C. The host range of the pathogen is very wide to damage plants such as eggplant, rape, chrysanthemum, liliaceae, apiaceae. In addition, the pathogen is capable of long-term survival in the soil and spreads through irrigation of bulbs and invades through wounds on plants. Occurs in high temperatures, high temperatures, and dry conditions.

Due to the pathogen, the tobacco sores are caused on the leaves, tobacco stalks and bulbs of tobacco. The cigarette scaly disease is caused by the rot of a needle on the leaf stem is gradually enlarged to soften the leaves and stems. If the tobacco scleroderma is severe, the whole leaves will yellow and eventually wither and die. The bulbs of tobacco affected by the cigarette sores recede to dark brown and rot and die with the ground.

Plant growth promoter according to the invention the Bacillus megaterium KNUC251 ( Bacillus megaterium KNUC251) or the culture filtrate of the strain. Plant growth promoting effect of the strain is to appear independently of the effects of the plant disease control described above. The plant growth promoter promotes the growth of the dicotyledonous plant, the dicotyledonous plant is selected from the group consisting of pepper, tobacco, tomato. The dicotyledonous plants have been described in the description of the plant disease control agent, but the description thereof will be omitted since they are the same as those described above.

Hereinafter, the present invention will be described with reference to preferred embodiments of the present invention. However, the following examples of the present invention are only for better understanding of the present invention and do not limit the protection scope of the present invention.

[ Example  One. ISR  And plant growth-inducing microorganisms Bacillus Megaterium KNUC251  Strain ( KCTC  11177BP) Separation Identification]

Bacillus megaterium KNUC251 strain according to the present invention has been deposited in the Genetic Resources Center in the Korea Institute of Science and Technology Biotechnology Research Institute, the depositing institution of the Korean patent strain on August 28, 2007 (Accession No .: KCTC11177BP). After collecting the peppers of the Daegu region, the strain was heat-treated (root and surrounding soil) of the pepper for 30 minutes at a temperature of 60 ℃ to isolate only spore-forming bacteria. When the suspension of the isolated strain was applied to the pepper and inoculated with bacterial spot pathogens, the strains showing the best ISR induction ability were selected when the degree of inhibition was observed. 16s rDNA sequencing of the strain showing the most excellent ISR inducing ability was identified as Bacillus megaterium of the present invention strain ( Bacillus megaterium ).

The 16S rDNA base sequence of Bacillus megaterium KNUC251 (KCTC 11177BP), the strain of the present invention, is shown in FIG. 1. The strain forms flesh-colored colonies when incubated at 37 ° C. for 12 hours in TSA (Tryptic Soy Agar) medium and sufficiently covers the surface of TSA medium after 24 hours of culture. However, the strain in the medium did not form a pigment.

[ Example  2. ISR  And plant growth-inducing microorganisms Bacillus Megaterium KNUC251  Strain ( KCTC  Culture of 11177BP)]

Bacillus megaterium KNUC251 strain (KCTC 11177BP) stored at minus 75 ° C. was passaged on TSA solid medium to reactivate the strain, and the strain was completely plated on 1/10 TSA medium surface, and at 24 ° C. at 37 ° C. Time incubation. The total amount of cells was 10 ¹³ to 10 ¹⁴ cfu (colony forming unit) / ml. The cells of the strain were put in 50 ml of sterile distilled water or 0.85% saline solution to prepare a culture solution at a concentration of 10 ⁷ to 10 ⁸ cfu / ml. ISR induction experiments were performed by placing the suspension of the cells on soil.

[ Example  3. Bacillus Megaterium KNUC251  Strain ( KCTC  11177 BP By treatment Plant disease  Inhibitory effect (1)]

In the case of control of bacterial bacterial spot disease of pepper, the pepper germinated from the plate was transferred to a 50-ball plug seedling plate, and after about two weeks, when the main leaves were about 4 sheets, the suspension of the strain was sprayed on the soil by 10 ml for each individual. After 5 to 7 days of pepper bacterium bacterium ( Xanthomonas) at a concentration of 10 ⁵ cfu / ml axonopodis pv. When the vesicatoria suspension was injected on the back of the pepper leaves, the symptoms began to appear after about 4-5 days, and the incidence rate of each treatment group was analyzed at levels of 0-5.

The ISR-derived chemicals BTH and ISR-derived strain E681 were used as positive controls, and distilled water treatment was used as negative controls. Results are shown in FIGS. 3 and 4. In the case of the strain, it was confirmed that the control effect of bacterial spot pattern disease of pepper than the known ISR induced strain E681.

[ Example  4. Bacillus Megaterium KNUC251  Strain ( KCTC  11177 BP ) Promoting Plant Growth by Treatment and Related Characteristics]

The plant growth promoting effect of the strain was measured by the dry weight of the pepper is shown in Figure 5 the results. The dry weight was measured 3 weeks after application of the strain suspension, and the ground and underground parts of red pepper were measured. Compared with the dry weight of the control, the dry weight of the ground was 116.4%, the underground was 152.1%, and the overall dry weight was increased by 123.5%. Based on this result, nitrogen fixation ability, phosphorylation ability, and auxin production performance were investigated to determine the plant growth promoting properties of the strain, and the results are shown in Table 1.

  Nitrogen-fixing ability +++  Phosphate solubility +++  Auxin productivity  7.9 μg / ml

The growth promoting effect of the tobacco by the strain was more dramatic than the control (control) and is shown in the photograph in FIG. ISR-derived compounds, including BTH, have been shown to exert superior plant disease control effects but adversely affect plant yield and growth (fitness cost). However, when treating the strain of the present invention did not show this problem, it was confirmed that this effect is superior to the known ISR and plant growth promoting strain GB03.

[ Example  5. Bacillus Megaterium KNUC251  Strain ( KCTC  11177 BP By treatment Plant disease  Inhibitory effect and growth promoting effect (2)]

To more specifically determine the properties of the ISR determinant produced by the strain germination of tobacco seeds as shown in Figure 2 was carried out the following experiment. Referring to FIG. 2, the tobacco seeds were sterilized, rinsed three times with sterile water, the seeds were scattered in MS medium, and germinated at 20 ° C. The germinated tobacco seeds were planted by moving five individuals on one side of the split I-Plate so that only the volatile substances produced by the strain could affect tobacco. On the other side, a sterile paper disc (size: 8 mm) is placed on the wall edge of the I-Plate, and three seeds are planted at a fixed distance (2 cm) to produce a secondary metabolite, i.e., a medium. The effect of the substance directly diffusing in the phase can be seen. 20 μl of the strain suspension (10 ⁸ cfu / ml) was dropped on a paper disc and sealed to grow plants in a 20-25 ° C. growth chamber for 2 weeks.

For verification ISR induced effects by the strain 10 ⁷ cfu / ㎖ tobacco sores germ density (Erwinia carotovora subsp. 6 μl of carotovora strain SCC1) suspension was dropped onto the whole tobacco leaf and examined for the number of leaves per subject 24 hours after pathogen treatment. As above, ISR-derived chemical BTH and ISR-derived strain GB03 were used as positive control and distilled water treatment was used as negative control. In the case of the treatment of the present invention, the control, including the known ISR-derived strain GB03 was found to be superior to the control of tobacco sores, which was similar to the degree of inhibition of symptom with the BTH treatment.

On the other hand, the defense system of plant diseases by the ISR is currently in the early stages of development in the world, there is no current product based on such technology. Bacillus megaterium KNUC251 ( Bacillus megaterium If the defense system of plant diseases by ISR induced by KNUC251) is established, it is possible to control various plant diseases only by treating the suspension of the strain, and these ISR substances by microorganisms are resistant to the induction resistance by conventional synthetic materials. Compared with the minimum impact on the yield and growth of plants can minimize the disadvantages of conventional chemical synthesis derivatives.

Specifically, plants that can be used according to the invention of the Bacillus megaterium KNUC251 strain (KCTC 11177BP) are dicotyledonous plants, preferably can be used in the group consisting of pepper, tobacco, tomato.

Plant disease control agent and plant growth promoter containing the Bacillus megaterium KNUC251 strain (KCTC 11177BP) or strain filtrate according to the present invention is environmentally friendly because it does not use organic chemicals. In addition, it is a useful invention that can increase the yield of plants by efficiently controlling plant diseases and at the same time promoting plant growth.

Although the present invention has been described with reference to one embodiment shown in the drawings, this is merely exemplary, and those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. . Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.

1 is a detailed view showing the 16S rDNA base sequence of Bacillus megaterium KNUC251 strain (KCTC 11177BP);

2 is a plan view briefly showing the germination of tobacco seeds on the medium to examine the plant disease inhibitory effect and growth promoting effect of Bacillus megaterium KNUC251 strain (KCTC 11177BP);

Figure 3 is a graph showing the results of evaluating the control effect of bacterial spot pattern disease of red pepper by the Bacillus megaterium KNUC251 strain (KCTC 11177BP) at the level of symptoms;

4 is a photograph showing the control effect of bacterial spot pattern disease of red pepper by the Bacillus megaterium KNUC251 strain (KCTC 11177BP);

5 is a graph showing the growth promoting effect of pepper by the Bacillus megaterium KNUC251 strain (KCTC 11177BP) by measuring dry weight;

Figure 6 is a graph showing the results of evaluating the control effect of tobacco softwood by Bacillus megaterium KNUC251 strain (KCTC 11177BP) in the number of leaves per cigarette individual A is 2 produced by Bacillus megaterium KNUC251 strain (KCTC 11177BP) This is a graph showing the control effect of tobacco chorea by tea metabolites (substances that diffuse into the liquid phase in the medium), and B is the control effect of tobacco chorea by the volatiles produced by the Bacillus megaterium KNUC251 strain (KCTC 11177BP). Graph shown;

Figure 7 is a detailed view showing the control effect of tobacco purine by Bacillus megaterium KNUC251 strain (KCTC 11177BP) photograph; And

Figure 8 is a detailed view showing the growth promoting effect of tobacco by the Bacillus megaterium KNUC251 strain (KCTC 11177BP).

<110> KYUNGPOOK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION <120> Bacillus megaterium KNUC251 for controlling plant disease and          accelerating plant growth, and plant disease controlling agent          and plant growth accelerant using the same <130> 1036274 <160> 1 <170> KopatentIn 1.71 <210> 1 <211> 1245 <212> DNA <213> Bacillus megaterium KNUC251 <400> 1 tagatgggat acttcgggaa ccgaagctaa taccggatag gatcttctcc ttcatgggag 60 atgattgaaa gatggtttcg gctatcactt acagatgggc ccgcggtgca ttagctagtt 120 ggtgaggtaa cggctcacca aggcaacgat gcatagccga cctgagaggg tgatcggcca 180 cactgggact gagacacggc ccagactcct acgggaggca gcagtaggga atcttccgca 240 atggacgaaa gtctgacgga gcaacgccgc gtgagtgatg aaggctttcg ggtcgtaaaa 300 ctctgttgtt agggaagaac aagtacaaga gtaactgctt gtaccttgac ggtacctaac 360 cagaaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg gcaagcgtta 420 tccggaatta ttgggcgtaa agcgcgcgca ggcggtttct taagtctgat gtgaaagccc 480 acggctcaac cgtggagggt cattggaaac tggggaactt gagtgcagaa gagaaaagcg 540 gaattccacg tgtagcggtg aaatgcgtag agatgtggag gaacaccagt ggcgaaggcg 600 gctttttggt ctgtaactga cgctgaggcg cgaaagcgtg gggagcaaac aggattagat 660 accctggtag tccacgccgt aaacgatgag tgctaagtgt tagagggttt ccgcccttta 720 gtgctgcagc taacgcatta agcactccgc ctggggagta cggtcgcaag actgaaactc 780 aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc 840 gaagaacctt accaggtctt gacatcctct gacaactcta gagatagagc gttccccttc 900 gggggacaga gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt 960 taagtcccgc aacgagcgca acccttgatc ttagttgcca gcatttagtt gggcactcta 1020 aggtgactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct 1080 tatgacctgg gctacacacg tgctacaatg gatggtacaa agggctgcaa gaccgcgagg 1140 tcaagccaat cccataaaac cattctcagt tcggattgta ggctgcaact cgcctacatg 1200 aagctggaat cgctagtaat cgcggatcag ctgccgcgga tctcc 1245  

Claims (8)

Bacillus megaterium KCTC 11177BP strain activates induction resistance-defense mechanism against pepper or tobacco. A plant disease control agent capable of preventing and treating bacterial spotty disease or tobacco purine disease of red pepper comprising the strain of Bacillus megaterium KCTC 11177BP of claim 1 or the culture filtrate of the strain. A pepper and tobacco plant growth promoter comprising the strain of Bacillus megaterium KCTC 11177BP of claim 1 or a culture filtrate of the strain. delete delete delete The method of claim 1, The strain is Bacillus megaterium KCTC 11177BP strain, characterized in that incubated for 12 hours to 24 hours at 30 ℃ to 60 ℃ on TSA (Tryptic soy agar) medium. The method of claim 7, wherein The strain is Bacillus megaterium KCTC 11177BP strain characterized in that it is incubated for 12 hours to 24 hours at 37 ℃ on TSA (Tryptic soy agar) medium.

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