KR100927970B1 - Sebum secretion containing acryl extract as an active ingredient and acne relief cosmetic composition - Google Patents

Abstract

The present invention relates to a composition for inhibiting activity of 5α-reductase containing a twill extract as an active ingredient. In addition, the present invention suppresses the production of dihydrotestosterone by inhibiting the activity of 5α-reductase, and when used as an active ingredient of the cosmetic composition, it is excellent in the effect of inhibiting excessive secretion of sebum and improving acne. Therefore, the twill extract according to the present invention can be used at an effective concentration in sebum secretion or acne relief cosmetics because it has an acne relief effect and sebum secretion inhibitory effect at a safe concentration on the skin.

Twill Extract, 5α-Reductase, Acne, Sebum Inhibition, Cosmetic Composition

Description

Cosmetic composition for inhibiting sebum secretion and acne relieving acne as an active ingredient {Cosmetic Compositions for Inhibiting Secretion of Sebum and for reducing an Acne containing Extract rapa japonica As Active Ingredient}

The present invention relates to a composition for inhibiting 5α-reductase activity, containing a twill extract as an active ingredient.

In addition, the present invention relates to a cosmetic composition for inhibiting sebum secretion and acne alleviation by containing a twill extract having a 5α-reductase inhibitory ability.

5-α-reductase is present in testosterone-reactive tissues such as sebaceous glands, hair follicles, prostate, and epididymis, reducing testosterone, one of the testosterones, to dihydrotestosterone (DHT). As an enzyme involved, its conversion requires NDPH (NADPH). Testosterone is involved in male impulses, skeletal muscle growth, male external genitalia, scrotum growth, and spermatogenesis, and dihydrotestosterone is involved in acne, sebum, and prostatic hyperplasia (Diane et al; JID 775-778, 1995. Bruchovsky, N et al; JBC 243, 2112-2121, 1968). Therefore, studies are actively underway to develop acne therapy and sebum secretion inhibitors using inhibitors of 5α-reductase enzyme.

In general, sebum in the skin, including the scalp and face is responsible for maintaining the skin's moisturizing and microorganisms invasion, but after puberty causes acne. In particular, women in their 20s and later causes a lot of problems in terms of beauty, such as skin swelling, lifting of makeup and widening pores due to excessive secretion of sebum. Sebaceous excess is caused by various causes, the most important of which is the sebum gland is activated by the amount of dihydrotestosterone, one of the hormones involved in promoting sebum secretion, sebum hypersecretion It is known.

Acne is not a single cause, but a combination of various causes. Hyperplasia of the sebaceous glands is active due to hyperactivity of pubertal hormones, epithelial cells of hair follicles are blocked, and clogged hair follicles. Is formed. Bacteria that reside in hair follicles produce free fatty acids that stimulate hair follicles to cause inflammation. This progression to acne is exacerbated by sebum oversecretion.

For the treatment of acne due to such testosterone suppression, female hormones such as estrogen have been conventionally used or 5α-reductase inhibitors have been used. However, these female hormonal agents, in addition to the treatment of acne, skin inflammation, side effects caused by the administration of hormones have been reported, the current situation is discontinued or used in very small amounts. On the other hand, it is commercially available as a preparation containing 15 to 20 azeleic acid (azelaic acid) inhibitor of 5α-reductase as an acne treatment agent that inhibits sebum oversecretion.

On the other hand, even in the cosmetic field, sebum oversecretion is a factor of interest as a factor causing the lifting of the cosmetics and widening of the pores, but the study is insignificant. Currently, sebum inhibiting cosmetics use a porous powder that temporarily adsorbs sebum or ivy extract, which is a herbal extract known to inhibit sebum, as a cosmetic raw material, but its effect is insignificant.

Accordingly, the present inventors have tried to find a natural extract that inhibits the activity of 5α-reductase, an enzyme catalyzing dihydrotestosterone, in order to control the amount of dihydrotestosterone known to cause sebum secretion and acne production. The present invention was completed by finding that the striation among various natural products is particularly excellent in inhibiting effect of 5α-reductase, sebum secretion effect and acne relieving effect.

Accordingly, it is an object of the present invention to provide a composition for inhibiting 5α-reductase activity, which contains a twill extract as an active ingredient.

In addition, another object of the present invention to provide a cosmetic composition for inhibiting sebum secretion or acne relief containing the active ingredient extract as an active ingredient.

The twill extract provided by the present invention not only has an excellent 5α-reductase inhibitory effect, but also a cosmetic composition containing the same, which has an excellent effect of inhibiting sebum oversecretion and improving acne, and hardly irritating the skin. Therefore, since the cosmetic composition containing the twill extract of the present invention as an active ingredient has sebum secretion suppression and acne relieving effect at a concentration that does not affect the product, it can be effectively used for sebum secretion inhibiting cosmetics or acne relief cosmetics.

The composition for inhibiting 5α-reductase activity, the cosmetic composition for inhibiting sebum secretion, and the acne-relieving cosmetic composition provided in the present invention contain the active ingredient as an active ingredient.

Trapa japonica is a dicotyledonous plant, a yearly herbaceous plant with two-leaved plated cranberries, growing in ponds or marshes, roots in mud, long stems, and floating on water. The leaves appear to be clustered and have thick areas on the petioles, which allow them to float on the water as air pockets. The leaf body is a lozenge-like triangle with fine jagged teeth. Feather-like roots come from the nodes of the main stem in the water.

A white or somewhat reddish flower blooms in the axilla in July-August, about 1cm in diameter, with 4 petals. Flowers are short and up, but bend downward as the fruit grows, 2 ~ 4cm long. The calyx leaves are hairy, with 4 stamens and 1 pistil. Fruits are hard, inverted triangular, with thorns with calyx change at both ends, with central part prominent.

Seeds are contained one by one and eaten. In folklore, berries are used for antidote and stomach cancer. Eating too much dryness only causes abdominal swelling, and drinking ginger juice can help prevent this. The dry leaves are used when the afl of a child is broken down, the rind is used for dysentery, diarrhea, prolapse and hemorrhoids, and the stem is used to treat gastric ulcers. Dried starch is also used as a yellow wine.

It is known to have anticancer activity as an annual herb that grows in water because its leaf stem is thick and hollow. Originally, this fruit is called medicinal herb and yield. In the past, it was steamed or boiled and eaten to boil porridge. The Medicinal Plant Dictionary says that if you eat dried fruit, it will not only heal the window, loosen the wine, brighten your eyes, but also cure stomach and uterine cancer. Drinking three times is effective in all kinds of cancer, eliminating alcohol poisoning and poisoning is said to be well digested.

In China, experiments showed that water-sweetened water in the ascites and liver cancers of rats had a certain inhibitory effect, and that 60% percent of sarcoma-180 cancers in rats had a certain inhibitory effect.

The dried fruit has anti-cancer activity on its bark, so the stomach, esophageal, and uterine cancers can be dried with powdered water or honey water, and the various kinds of cancers are dried with three pieces of dried fruit, yeolmu, burnt vines and wisteria lumps. The leaves and stems are also sweetened with tea, so it is good to drink from time to time.

The book The Secrets of Home Nursing in Japan states that dried fruit is placed in a bowl made of earth and dried on low heat for 3 to 4 times a day. have.

In the cosmetic composition of the present invention, the twill extract means that the extract is obtained by a conventional method from various organs such as leaves, stems, roots, flowers, and fruits of the twill plant, preferably an extract obtained from the fruits, stems, roots and leaves. Means.

The twill extract of the present invention may be prepared according to conventional methods known in the art, i.e., under the conditions of conventional temperature and pressure, using conventional solvents.

The twill extract may be selected from a variety of extraction solvents, such as water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol and butanol), acetone, ethyl acetate, chloroform, 1,3-butylene glycol and butyl It can be obtained as an extraction solvent selected from the group consisting of acetate, preferably obtained by using a suitable mixture of water and ethanol, more preferably obtained by using a mixed solution of 3: 7 mixed with water and ethanol will be. On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained in addition to the above-described extraction solvents, extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The active fraction is also included in the extract of the present invention.

According to a preferred embodiment of the present invention, the content of the twill extract is 0.0001 to 10% by weight, preferably 0.5 to 5%, most preferably 1.0 to 3.0% by weight relative to the total weight of the cosmetic composition.

If the content of the twill extract is less than 0.0001% by weight, there is a problem that the effect by the twill extract can not be expected, and when the content of the twill extract exceeds 10% by weight, there is no apparent increase in effect due to the increase in content, but may cause skin irritation. And there is a problem in the stability of the formulation.

The components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions in addition to the twill extract as an active ingredient, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and Carrier.

The cosmetic composition of the present invention may further include a thickener, a skin protectant, a moisturizer, a neutralizer, a pH adjuster, a solubilizer, a preservative, and a solvent as needed in addition to the above components.

As used herein, "acne" includes any skin disease known in the art as acne or any skin disease invented through a mechanism similar to acne. Examples include, collective acne, bromide acne, common acne, congoblate acne, acne from cosmetic use, acne dtergicans, adolescent acne, acne fulminans, and aging Acne furunculoid, acne caused by halogen, acne acne, keloid acne, mechanical acne, drug acne, necrotic acne, lip acne, neonatal acne neonatorum, oily acne (acne oil), papular acne, pomade acne, premenstrual acne, acne rosacea, swelling acne, tropical acne, acne venenata, and vulgar acne. Include.

The sebum secretion reducing or acne improving composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powder soaps, Surfactant-containing cleansing, oils, powders, foundations, emulsions and foundations, wax foundations, sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples and Experimental Examples. These examples are only for illustrating the present invention in more detail, it is to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example 1 Preparation of Twill Extract

1 kg of finely cut twill fruit, stem, root and leaf were immersed in an aqueous solution mixed with ethyl alcohol and water at 7: 3 (v / v) for 5 days, filtered through a 300 mesh filtration membrane, and then vacuum reduced. Concentrated at 80 ° C. using a concentrator. The twill powder thus obtained was used after being dissolved in 1,3-butylene glycol to be easily applied to the following experimental examples or products.

Experimental Example 1. 5α-Reductase activity inhibitory effect test

This experiment was carried out to determine whether the extract of the twill is an inhibitor that inhibits the activity of 5α-reductase.

1-1. Preparation of Enzyme Liquid

A 24-hour fasted Sprogue-Dawley rat (7-8 weeks old) was killed with diethyl ether, and the liver was ice-cold in Crebs-Ringer phosphate buffer (pH). Perfusion). Five times of ice-cold Tris HSI buffer (Pri-HCL buffer, pH 7.2) was added thereto and homogenized, followed by centrifugation at 900Xg for 10 minutes. The upper layer was centrifuged at 5000Xg for 10 minutes, and the upper layer obtained when the upper layer was centrifuged at 9000Xg for 10 minutes was used as the enzyme solution. The enzyme solution was cryopreserved at 80 ° C.

1-2. Inhibitory effect test of 5α-reductase

0.3 ml of testosterone (500 ug / ml) in 1.0 ml of Tris-Hcl buffer (pH 7.2), twill extract prepared in Preparation Example 1 at a concentration of 0.1, 0.2, 0.5, 1%, and enzyme solution 1.0 The ml was mixed, 0.5 ml of NADPH (0.77 mg / ml) was added to initiate the reaction, and the reaction was carried out at 37 ° C for 30 minutes. 5 ml of dichloromethane was added to stop the reaction. 0.5 ml of an internal standard (p-hydroxybenzoic acid n-hexyl ester, 0.1 mg / ml) was added thereto, shaken for 10 minutes, and centrifuged at 3000 rpm for 10 minutes. After the upper layer was removed, a dichloromethane layer was left, and 5 ml of methanol was added thereto to prepare a sample for high performance liquid chromatography (HPLC).

5α-reductase inhibition rate was calculated according to the following formula 1 after measuring the amount of testosterone of each experimental group using the HPLC method, the results are shown in Table 1.

* cont 0: without adding succinct extract and adding dichloromethane before adding NADPH to avoid reaction

* cont 1: done without addition of the intact extract

Twill Extract (%) 5α-reductase inhibition rate (%) 0.0001  9.06 0.001 24.9 0.01 47.3 0.02 61.7 0.05 88.3 0.10 93.5

As can be seen from the results of Table 1, it was confirmed that the twill extract inhibited the activity of 5α-reductase from about 0.1% to 93.5%, thereby inhibiting the activity of 5α-reductase depending on the treatment concentration. . Therefore, it was found that the stalk extract that inhibits 5α-reductase activity has an effect of suppressing the amount of testosterone dihydrosterone produced.

Experimental Example 2

Treating a cotton-causing agent in the rabbit's ears will cause the cotton to grow after one week, but the larger cotton usually looks the same as before acne's inflammation. As the scalp grows, there is an excess of sebum in the pores, which causes the pores to become larger than normal. Thus, the scum outbreak experiment is somewhat different from the acne effect in humans, but it is the experimental model that is most similar to acne at present.

In order to check whether the twill extract prepared in Preparation Example 1 has a scrim suppression ability, the following experiment was performed.

Applying 200ul of 50% oleic acid / paraffin oil, which is a cotton-causing substance, to the rabbit's ears once a day to induce cotton cloth, and simultaneously extract the twill extract prepared in Preparation Example 1 as a cosmetic raw material and not causing cotton cloth. Dissolved in 1% in BG, 200ul was applied twice a day. Experiments were performed by treating oleic acid as a control group and setting the group without applying the twill extract (control). The number of cotton cloth was based on the size of the cotton cloth being 0.2 mm or more. After exfoliation of the tissue of the scleroderma site by 0.8 mm, the size of the scrim was measured by an optical microscope. In order to measure the size of the cotton wool, A ~ E randomly selected six among the well-developed ones from the whole tissue and measured the size of the cotton cloth. The results are shown in Table 2.

The number of animals Cotton catcher Measured cotton cloth size A B C D E Average Oleic Acid Treatment Group (Control) n = 3 17 6 5 6 5 4 5.2 5.47 5 5 6 6 5 5.4 5 6 7 5 6 5.8 Oleic Acid Treatment + Twill Extract n = 3 8 3 3 3.5 4 2.5 3.2 2.83 2.5 3 3.5 3 2 2.8 3 2 3 2.5 2 2.5

As can be seen from the results of Table 2, the extract of the present invention effectively suppressed the generation of cotton cloth according to the oleic acid treatment. In other words, the treatment resulted in the small number and size of cotton cloths treated by the twill extract, suggesting that the twill extract induced less sebum secretion and inhibited the progression to acne.

Through the above experiments, it was indirectly confirmed that the twill extract inhibited the activity of 5α-reductase causing sebum secretion, thereby inhibiting sebum secretion, thereby effectively suppressing the occurrence of cotton blemishes and alleviating acne. Thus, in order to directly confirm whether the extract of the twill extract has sebum secretion and acne relieving effect, the following formulation example shows a formulation example of a cosmetic containing a twill extract, but not all prescriptions are limited thereto.

Formulation Example 1 Flexible Lotion

Formulation examples of the flexible lotion in the cosmetic containing the twill extract of Preparation Example 1 are as follows. Also for comparison, Comparative Example 1 containing no twill extract and Comparative Example 2 containing Chelidonium majus extract (Korean Patent Registration No. 720329), which are known to have sebum secretion efficacy, were used as a formulation example. Prepared.

Ingredient (% by weight) Example 1 Comparative Example 1 Comparative Example 2 Twill Extract 1.0 - - White bran extract - - 1.0 glycerin 6.0 6.0 6.0 1,3-butylene glycol 3.0 3.0 3.0 PEG 1500 1.0 1.0 1.0 Allantoin 0.1 0.1 0.1 DL-panthenol 0.3 0.3 0.3 EDTA-2Na 0.02 0.02 0.02 Benzophenone-9 0.04 0.04 0.04 Sodium hyaluronate 5.0 5.0 5.0 ethanol 10.0 10.0 10.0 Octicdodeceth-16 0.2 0.2 0.2 Polysorbate 20 0.2 0.2 0.2 Preservative, fragrance, coloring a very small amount a very small amount a very small amount Distilled water Remaining amount Remaining amount Remaining amount Sum 100 100 100

Formulation Example 2. Convergence Lotion

Formulation examples of the astringent lotion in the cosmetic containing the twill extract of Preparation Example 1 are as follows.

ingredient Content (% by weight) Twill Extract 1.0 glycerin 6.0 1,3-butylene glycol 3.0 Allantoin 0.2 DL-panthenol 0.2 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Witch hazel extract 2.0 Citric acid a very small amount Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Formulation Example 3 Nutritional Lotion

The formulation example of the nutritional lotion in the cosmetic containing the twill extract of Preparation Example 1 is as follows.

ingredient Content (% by weight) Twill Extract 3.0 Glyceryl Stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 1.3 Sorbitan stearate 0.5 Cured Vegetable Oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxyvinyl Polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaluronate 5.0 Triethanolamine 0.12 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Formulation Example 4. Nutrition Cream

Formulation example of the nutrient cream in the cosmetic containing the twill extract of Preparation Example 1 is as follows.

Ingredients (content: weight%) Example 2 Comparative Example 3 Twill Extract 3.0 - Lipophilic monostearate 2.0 2.0 Cetearyl Alcohol 2.2 2.2 Stearic acid 1.5 1.5 Beeswax 1.0 1.0 Polysorbate 60 1.5 1.5 Sorbitan stearate 0.6 0.6 Cured Vegetable Oil 1.0 1.0 Squalane 3.0 3.0 Mineral oil 5.0 5.0 Trioctanoine 5.0 5.0 Dimethicone 1.0 1.0 glycerin 5.0 5.0 Betaine 3.0 3.0 Triethanolamine 1.0 1.0 Sodium hyaluronate 4.0 4.0 Preservative, fragrance, coloring a very small amount a very small amount Distilled water Remaining amount Remaining amount Sum 100 100

Formulation Example 5. Massage Cream

Formulation example of the massage cream in the cosmetic containing the twill extract of Preparation Example 1 is as follows.

ingredient Content (% by weight) Twill Extract 3.0 Lipophilic Monostearic Acid Glycerin 1.5 Stearyl alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl Isosterate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Formulation Example 6 Pack

Formulation examples of the pack in the cosmetic containing the twill extract of Preparation Example 1 are as follows.

ingredient Content (% by weight) Twill Extract 3.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Chicdestrin 0.15 DL-panthenol 0.4 Allantoin 0.1 Glycyrisin Monoammonium 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 Cured Castor Oil 0.3 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Formulation Example 7 Essence

Formulation examples of the essence in the cosmetic containing the twill extract of Preparation Example 1 are as follows.

ingredient Content (% by weight) Twill Extract 3.0 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer 0.2 Triethanolamine 0.18 Octyl dodenkanol 0.3 Octyldodeceth-16 0.4 ethanol 6.0 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Experimental Example 3. Sebum secretion inhibitory effect

In order to determine whether the sebum secretion inhibitory effect of the cosmetic containing the extract of the present invention is sebum secretion in the 20s men and women with a large amount of sebum secretion was divided into three groups of A, B and C as follows.

The experiment was carried out using the formulations of Example 1 and Comparative Examples 1 and 2 prepared in Formulation Example 1, the flexible lotion of Example 1 containing the twill extract in Group A, and the twill extract was not included in Group B. In Comparative Example 1, group C, Comparative Example 2, which contains the extract of Sesame Seeds, known to have sebum secretion effect, was used every morning and evening for 4 weeks. Sebum measurement was stabilized in a constant temperature and humidity room for 30 minutes after washing the face, and then sebum was measured on the forehead by using a sebumeter. The sebum amount was set to an initial value before using the flexible lotion for the test, and the change in sebum amount was measured once a week for 4 weeks while using the flexible lotion continuously. The change of sebum amount by the extract of the present invention was determined whether the sebum inhibitory effect compared to the initial sebum amount, the results are shown in Table 10.

Sebum secretion (g / ㎠ / h) Example 1 Comparative Example 1 Comparative Example 2 Initial value 203.4 ± 15.9 201.8 ± 18.6 206.7 ± 17.3 2 weeks 140.3 ± 14.8 181.3 ± 19.6 146.1 ± 19.2 4 Weeks 120.9 ± 17.1 165.3 ± 18.5 131.2 ± 18.4

As can be seen from the results of Table 10, the flexible lotion of Example 1 containing the twill extract of the present invention effectively reduced sebum secretion over time compared to Comparative Example 1 containing nothing, When compared with Comparative Example 2 containing the white bran extract known to have a sebum secretion inhibitory effect was confirmed to show a similar result in the sebum secretion effect. Therefore, it is expected that the twill extract of the present invention can effectively prevent sebum secretion from occurring due to excessive secretion of sebum by effectively reducing sebum secretion.

Experimental Example 4: Acne Improvement

Ten men and women with acne were subject to apply the flexible lotion of Example 1 evenly throughout the face for 4 weeks in the morning and evening. The effect was judged by 1 to 3 points depending on the degree of feeling felt by the person who participated in the test.

<Evaluation before the test>

1 = some acne 2 = some acne 3 = severe acne

<Acne effect judgment>

-: Almost no effect +: Slight effect

++: Relaxed a lot +++: Completely healed

Pretest State Results after 2 weeks Results after 4 weeks Subject 1 3 + ++ Subject 2 3 - + Subject 3 3 + ++ Subject 4 2 + ++ Subject 5 3 ++ +++ Subject 6 2 - ++ Subject 7 2 + + Subject 8 3 + ++ Subject 9 2 + + Subject 10 3 + ++

As can be seen from the results of Table 11 above, as a result of using the flexible lotion of Example 1 containing the twill extract of the present invention for 4 weeks, the majority of subjects were able to confirm the improvement of acne.

Experimental Example 5. Evaluation of Safety of Twill Extract

In order to evaluate the stability of the cosmetic containing the twill extract of the present invention, the Sting test was performed as follows. The test was conducted on 15 subjects aged 25-33 years with an average age of 31.5 years. After a sufficient period of sweating for 15 minutes using a steam generator, a sufficient amount of the sample was buried in the hands, and Example 2 and Comparative Example 3, which were the nutrition creams prepared in Formulation Example 4, were placed around the nose and cheeks on the left and right sides, respectively. Rubbing strongly around. The degree of response was found to be within 30 seconds depending on the time of stimulation response after applying nourishing cream.Severe Irritation; within 2.5 minutes; Strong Irritation; within 5 minutes. Irritation) and cases appearing within 8 minutes were evaluated as classified as no irritation, and the reaction rate (%) and the determination result in terms of percentage are shown in Table 12.

Evaluation standard score No irritation 0.00 to 0.90 Moderate Irritation 0.91-2.50 Strong Irritation 2.51 to 5.00 Severe Irritation 5.00 ~

Comparative Example 1 Example 1 Reaction degree 45 43 % Reaction (n = 15) 1.25 1.14 Judgment Unstimulated Unstimulated

As can be seen from the results of Table 13, the cosmetic composition containing the extract of the present invention was found to be extremely weak skin irritation.

Claims (6)

delete Sebum secretion cosmetic composition for containing sesame extract as an active ingredient. Acne relieving cosmetic composition characterized in that it contains a twill extract as an active ingredient. The cosmetic composition according to claim 2 or 3, wherein the content of the twill extract is 0.0001-10% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 2 or 3, wherein the cosmetic composition is a solution, a suspension, an emulsion, a paste, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation and a spray Cosmetic composition, characterized in that it has a formulation selected from the group consisting of. A cosmetic method according to claim 2 or 3, wherein the cosmetic composition is applied to human skin to suppress sebum secretion and alleviate acne.