KR100902947B1 - Composition Containing Culture of Bacillus subtilis MORI for Growth Promotion - Google Patents

Abstract

The present invention relates to a composition for promoting growth in the growth-promoting effect, and gasiohgapi safflower, Yam, Cornus officinalis, of Poria cocos, overgrown antler and ginseng extract (herbal extract) and Bacillus subtilis memory (Bacillus subtilis MORI) The present invention relates to a growth promoting composition capable of promoting the growth of infancy and adolescence by mixing and containing a culture.

Bacillus subtilis mori, Prickly Pear, Herbal Medicine, Growth

Description

Composition for promoting growth containing Bacillus subtilis Mori culture {Composition Containing Culture of Bacillus subtilis MORI for Growth Promotion}

1 is a rat (Rat) rat growth test using physiological saline (control) group, prickly scabies and 6 kinds of Chinese herbal medicine (GSW) extract feed group, prickly scabies and 6 kinds of medicinal herb extracts and Bacillus culture feeding group, prickly scabies and 6 Herbal Medicine Extracts and Lactobacillus Culture Feeding Group, Prickly Ginseng and 6 Herbal Medicine Extracts and Surfactant Feeding Group and 6 Herbal Medicine Extracts and Bacillus Culture, Lactobacillus Culture and Surfactant (Sorbitan monostearate) This is a graph showing weight gain rate for 2 weeks for the mixed pay group.

Figure 2 is a growth test using rat rats physiological saline feeding group (control), Bacillus culture in conventional products and thorny opi and 6 kinds of medicinal herb extracts feed group (existing), conventional products and thorny opi and 6 medicinal herb extracts Vitamin AD ₃ and Surfactant Feeding Group (improvement 2), Existing Products and Prunus scabbard and 6 Herbal Extracts, Improved Bacillus Culture, Vitamin AD _This is a graph showing growth rate for 2 weeks with respect to the feeding group (improvement 3) mixed with ₃ and surfactant.

Figure 3 is a growth test using rat rat physiological saline feeding group (control), Bacillus culture in conventional products and thorny opi and six kinds of medicinal herb extracts feed group (existing), conventional products and thorny opi and six medicinal herb extracts Vitamin AD _{3 in} water-adjusted group (Improvement 1), extracts of conventional products and thorny opi and 6 kinds of herbal medicine, Bacillus culture, vitamin AD _{3 is} a diagram measuring IGF-1 for a surfactant-adjusted group (improvement 3).

The present invention is a growth promoting composition having a growth promoting effect, and more specifically, thorn ogapi and safflower seeds, acid pills, cornus, bokyeong, green and ginseng (hereinafter referred to as' safflower seed, mountain pills, cornus, bokyeong, green and ginseng '' 6 kind of medicinal plants "referred to.) extracts and Bacillus subtilis Mori (Bacillus of subtilis MORI) relates to a growth promoting composition capable of promoting growth by containing a culture (hereinafter referred to as 'bacillus culture').

'Growth' is a complex biological phenomenon that is a change in size accompanied by maturation, but the term commonly used refers to an increase in kidneys. An increase in kidneys is associated with cartilage tissue synthesis, skeletal length growth, and extensive bone growth in the skeleton. Made through skeletal metabolism, including (Crotton. PM. Et . al . (1997), Clin. Chem., 43 (9); 1577-81).

The main factor contributing to the growth is the increase in skeletal length. The increase in bone length is caused by cartilage within the cartilage, and the bone growth is caused by the conversion of cartilage tissue to bone tissue. Cartilage occurs mainly in the long bones of the extremities, and the increase in bone develops directly into the skeletal tissue in the fibrous membrane.

Although there are various indicators for assessing growth, the measurement indicators in animal studies measure the most weight (g), and bone growth through femoral length, weight, volume, density, area, mineral content, and mineral density. And bone density. Insulin-like growth factor-1 (hereinafter referred to as 'IGF-1') is a peptide hormone expressed in hepatocytes and released into the blood. Therefore, the measurement of the concentration of IGFF in the blood indirectly evaluates growth hormone secretion. (Former Jing Kyung et al. (2000), Korean Journal of Pediatric Endocrinology, 5 (1); 75-82)

IGF-1 acts similar to insulin by stimulating the synthesis of sugars, lipids and proteins in the target tissues of insulin under the influence of growth hormone. In other words, it affects growth by being involved in cell proliferation and differentiation. IGF-1 receptors are present in osteoblasts and chondrocytes. IGF-1 induces skeletal growth through the effect of chondrocytes, induces mitosis for osteoblasts, and collagen synthesis by osteoblasts. Promotes skeletal formation. IGF-1 is relatively easy to measure because it maintains a constant concentration in the body and is a useful diagnostic indicator for evaluating endogenous growth hormone secretion.

Increasing national income has attracted much attention to childhood growth, and the average height and weight of adolescents are gradually improving and improving in childhood, such as increasing nutritional intake levels, improving the living environment, and rationalizing lifestyles. As a result of the change of perception, a plan for promoting growth has emerged, and recently, researches on growth promotion extraction and pharmaceutical preparations containing the same have been conducted.

The thorny ogapi is known to contain carotene, ligustrin, etc., which are dried roots, roots and roots of bark, which is a deciduous shrub belonging to the oga family, and sedative action by pharmacological action , Antistress action, immune enhancing action, relaxation action, anti-inflammatory action has been reported. Various uses have been studied based on the pharmacological action of prickly pear, and it has been described that it treats acne and rashes. In the anti-stress composition (Korean Patent Registration No. 160402), prickly pear skin was used as a main ingredient.

In addition, growth promoting extracts containing the thorny scabies extract and pharmaceutical preparations containing the same (Korean Patent Publication No. 2003-30337) are known, but the degree of effect is still less than expected.

Thus, the present inventors found that the growth promoting effect is greatly improved by adding a Bacillus culture to the medicinal herb extract extracted from the thorny scabies and the six herbal medicines while studying the invention of the formulation with improved growth promoting effect. It was completed.

Accordingly, the present invention is to provide a growth promoting composition containing the thorny opi and the herbal extracts and Bacillus culture of the six herbs.

The present invention is 10-90% by weight, 0.1-50% by weight safflower seed, 0.1-50% by weight, wild corn oil 0.1-50% by weight, 0.1-50% by weight of Bokryeong, 0.1-50% by weight, green ginseng 0.1-50% It relates to a growth promoting composition comprising 100 parts by weight of the herbal extract extracted from the mixture comprising a weight percent and 0.1-10 parts by weight of Bacillus culture.

The present invention also relates to a growth promoting composition, characterized in that the composition is in a dry or liquid form.

Hereinafter, the present invention will be described in more detail.

First, Bacillus subtilis mori ( Bacillus) used in the present invention subtilis MORI) is a bacterium similar to that used for soybean fermentation. It has excellent starch resolution, excellent protein resolution, and breaks down carbohydrates of fats and polymers, and has low molecular weight amino acids, nucleic acids, fatty acids, vitamins, alkaloids, and viscosities. It has the characteristic of producing polymer, etc., and it is very beneficial for promoting growth when humans or animals ingest liquid or dried product of the culture, and it is traditionally safe bacteria or culture.

Next, prickly oleracea is a deciduous shrub belonging to the Araliaceae, and the dried roots, rhizomes, and bark of Acanthopanax senticosus (RUPR. Et MAXIM) HARMS are carotene, ligustrin, 7-methyl- 6,8-dimethylcoumaringlycoside, galactoside, syringaresinol di-o-beta-D-glucoside ) And various glycosides such as caryophyllenol, chlorogenic acid, flavone, essential oil and polysaccharide.

And, safflower seeds are effective in strengthening bones and strengthening the muscles.

The medicine is used to treat the symptoms of non-herb diarrhea, old diarrhea, seawater, small salt, oil well, lobster, urine binsak, with the efficacy of dry rain, abolism, high body, and Ikjeong. The main ingredient is saponin, mucus, choline, starch, glycoprotein, and free amino acid.

Cornus is a Chinese medicine commonly used to treat symptoms such as Hyun-hoon, Tinnitus, Yoshinan colic, Urinary well, Urinary tract, Urinary tract, and Bubodae, due to the efficacy of Boik Ganshin and eclampsia. Cornin, verbenalin (verbenalin), saponin (saponin), tannin (tannin), ursolic acid (ursolic acid), malic acid, tartaric acid and other ingredients are contained.

Bokryeong is an herbal medicine that has been commonly used to treat symptoms such as water urinary tract, Damdam Hyeon-hoon, unhavened place, diarrhea, and mental and anxiety with the efficacy of Isu Samsu and Geunbinyeongsim. 7.5 wt% of β-pacyman and triterpenoid compounds, pachymic acid, ebricoic acid, tumulosic acid, and pinicoric acid ( pinicolic acid).

Green rust is a herb that has been commonly used when both stomach oil and knee joints are cold and ill due to the efficacy of Onsinyang, Kang Geungol, and hemangioma. The main components are colloid, calcium phosphate, Calcium carbonate and the like.

Ginseng is a herbal medicine used for the symptoms of overwork, reduced meals, lethargy, forgetfulness, gastritis, and thirst due to the effects of Daebowongi, Gotalengjin, and Anshin. Ingredients, beta-elemen, palmitic acid, stearic acid, olein acid, olein acid, linol acid, and other ginseng acids such as cholin, protein, and polysaccharides It is contained.

Hereinafter, a method of preparing a Chinese herbal extract from the mixture of thorn ogapi and the six herbal medicines will be described.

First of all, 10-90% by weight, 0.1-50% by weight safflower, 0.1-50% by weight, 0.1-50% by weight of cornus oil, 0.1-50% by weight of fuxing, 0.1-50% by weight of green tea, ginseng Weigh to 0.1-50% by weight and place it in the extraction tank.

Next, 1-24 hours at room temperature using 20-100% alcohol solution (methanol, ethanol or butanol), ethyl acetate or water as an extractant, which is 2-20 times (W / V) of the total raw materials. And then extracted for 8-12 hours. In this case, the extraction temperature is preferably 60-80 ° C. when 20-100% alcohol solution or ethyl acetate is used, and 90 ° C.-100 ° C. when the extraction is performed using water.

After the extraction is completed, the first step is to remove the medicinal herbs and the remaining filtrate using a micro filter (Filter) or a filter press (Filter Press) to remove insoluble substances such as residues remaining in the extraction solvent, and then separated The extract is concentrated using a reduced pressure concentrator so that the solid concentration is 60-70% by weight.

Finally, the prepared concentrate is freeze-dried using a lyophilizer and pulverized to prepare a powder. The yield of the freeze-dried extract powder is 5-20% (W / W) of the weight of the herbal medicine added at the beginning of the extraction process. It is preferable to become).

However, the herbal extract extraction process may be used conventional ultrasonic extraction, filtration, reflux extraction or reduced pressure concentration method, in addition to the conventional extraction method may be used, concentration and freezing process is also a variety of commonly known methods Can be used.

In addition, the extract may be applied in the form of concentrate, extract, freeze-dried powder, depending on the formulation of the product to be prepared (type of powder, tablet, liquid, etc.).

Next, the manufacturing method of a Bacillus culture is demonstrated.

The Bacillus culture is cultured in sterile medium. The medium may be a conventional medium, preferably 0.5 to 5% by weight of carbohydrate components such as corn starch, 0.2 to 2% by weight of protein components such as soy flour, 0.2 to 0.7% by weight of yeast extract, 0.05 to 0.2% by weight of a phosphoric acid component such as potassium phosphate may be contained. After inoculating Bacillus subtilis MORI bacteria to the medium, the aeration amount and stirring speed of 0.05 to 0.4 vvm (vol / vol / min.) For 2 to 5 days at 30 to 45 ° C and agitation speed of 100 to 200 rpm ( rotary / min.), and may be incubated at an internal pressure of 0.1 to 1 kgf / cm 2. The pH of the medium may be 6.0 to 9.0. The cultured Bacillus culture is preferably centrifuged in a centrifuge and then concentrated by 5 to 15 times and dried by adsorbing to 3 to 7 times calcium carbonate or dextrin.

Finally, the prepared herbal extract and the Bacillus culture are mixed, so that the content of the final composition is 100 parts by weight of the herbal extract and 0.1-10 parts by weight of the Bacillus culture.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following examples.

< Production Example  1> Preparation of Prickly Pear and Six Herbal Medicine Extracts

10 times the total weight of the raw material (50% by weight of shiogapi, 14% by weight of safflower seed, 6.5% by weight of acidic acid, 6.5% by weight of cornus oil, 10% by weight of Fukryeong, 6.5% by weight of green tea, 6.5% by weight of ginseng) 50% (v / v) drinking water solution corresponding to w / v) was used as an extraction solvent and soaked for 1 hour. Ultrasonic extraction for 5 hours while maintaining at 50 ℃, overnight extraction (10 hours) at 70 ℃ after the extraction, the supernatant was filtered through a micro filter. The filtered extract was concentrated to a solid concentration of 65% by weight. The concentrate was dried with a lyophilizer and ground to prepare a powder. After lyophilization, more than 10% (w / w) of the weight of the initially added herbal medicine was obtained.

< Production Example  2> Bacillus  Preparation of Culture

Bacillus subtilis MORI KCCM 10450 is a medium containing 2% by weight of corn starch, 1.0% by weight of soy flour, 0.5% by weight of yeast extract, 0.1% by weight of potassium monophosphate, and 0.02% by weight of antifoaming agent (pH). 7.5) and incubated for 3 days at 250rpm, 37-38 ℃, aeration 0.3vvm conditions, and then put calcium carbonate at 10% of the culture medium weight and stirred well inlet temperature 120 ℃, outlet (outlet) Bacillus cultures were prepared by spray drying at a temperature of 85 ° C.

< Production Example  3> Preparation of Lactic Acid Bacteria Culture

Lactobacillus helveticus ) bacteria 1% by weight peptone, 1% by weight meat extract, 0.5% by weight yeast extract, 2% by weight glucose, sorbitan monooleate 0.01%, ammonium citrate 0.5%, sodium acetate 0.01%, magnesium sulfate 0.01%, sulfate Inoculate in medium of 0.005% manganese and 0.2% potassium diphosphate (pH 6.5), shake culture at 120rpm for 3 days at 37 ℃, centrifuge at 8,000rpm to obtain only Lactobacillus, and 30% of the original weight. After putting milk (skim milk) was prepared by freeze drying.

[ Experimental Example ]

In Examples 1 to 2, SD male rats (3 weeks old) were purchased (Samtaco, South Korea) and adjusted to an animal laboratory by adjusting the temperature to 22 ° C., 50% humidity, and 12 hours of day and night for 5 days. The total test period was 2 weeks, and body weight and feed intake were measured once daily.

< Experimental Example  1> Growth Promotion Test

  Each test group was composed of SD rats of the same age and sex. As shown in the table below, 1) control (administration of saline only), 2) mixed squid and 6 kinds of herbal medicine, 3) mixed squid and 6 kinds of herbal medicine and Bacillus culture, 4) squid and 6 kinds of herbal medicine And a mixture of the lactic acid bacteria cultures, 5) a mixture of treated with a surfactant (Sorbitan monooleate) in the Gasiogapi and six kinds of herbal medicines, 6) a mixture of Gasiogapi and six herbs, the surfactant in the lactic acid cultures and the Bacillus culture The mixture was divided into treated spheres and performed as Table 3 below. As a result, 3) the weight gain rate was the highest in the mixed diets of psigapi and the six medicinal herbs and Bacillus cultures (see FIG. 1). In the case of serum IGF-1 content, all the test groups were better than the control group. Especially, the mixture treated with surfactant was the highest in Gasiogapi, six herbs, lactic acid bacteria cultures and Bacillus cultures (see Table 4). In addition, the maturation length of the Bacillus culture mixture was shown to be significantly longer in the thorn stem and 6 kinds of Chinese herbal medicine (see Table 5).

* [Table 1]

Test Dosage Long bone length (mm) Upper iliac width (mm) Long bone medium width (mm) Lower iliac width (mm) Iliac weight (mg) Control (saline) 100 mg / kg 27.72 ± 0.80 6.10 ± 0.08 2.42 ± 0.07 3.49 ± 0.05 265 ± 9.26 Gogagapi and 6 kinds of herbal medicine 100 mg / kg 28.11 ± 0.25 6.12 ± 0.08 2.40 ± 0.06 3.46 ± 0.05 262 ± 8.38 Ogapi and 6 Herbs, Bacillus Culture 100 mg / kg 5mg / kg 28.39 ± 0.20 6.03 ± 0.06 2.26 ± 0.05 3.50 ± 0.04 260 ± 5.69 Ogapi and 6 Herbs, Lactic Acid Bacteria Culture 100 mg / kg 5 mg / kg 28.14 ± 0.25 6.05 ± 0.04 2.33 ± 0.07 3.40 ± 0.05 255 ± 7.73 Prickly Pear and 6 Herbs, Surfactants 100 mg / kg 5 mg / kg 28.44 ± 0.24 6.26 ± 0.10 2.33 ± 0.06 3.57 ± 0.04 * 259 ± 8.47 Prickly Pear and Six Herbs, Surfactants, Lactic Acid Bacteria Culture, Bacillus Culture 100 mg / kg 5 mg / kg, 5 mg / kg, 5 mg / kg 27.99 ± 0.18 5.91 ± 0.07 2.20 ± 0.04 3.37 ± 0.04 255 ± 6.82

*; p <0.05

The method of measuring iliac growth was anesthetized with experimental rat SD rats orally administered for two weeks, and then, the left and right iliac bones were separated, and total length, width (upper, middle, lower) and weight were measured using calipers.

TABLE 2

Test IGF-1 (ng / ml)  Control 1508 ± 9  Gogagapi and 6 kinds of herbal medicine 1530 ± 6  Ogapi and 6 Herbs, Bacillus Culture 1677 ± 7  Ogapi and 6 Herbs, Lactic Acid Bacteria Culture 1613 ± 1  Prickly Pear and 6 Herbs, Surfactants 1565 ± 3  Prickly Pear and Six Herbs, Surfactants, Lactic Acid Bacteria Culture, Bacillus Culture 1819 ± 23 *

*; p <0.05

In the blood IGF-1 analysis method, anesthetized the experimental animals SD rats administered orally for 2 weeks with ether, followed by orbital collection for 1 ml or more in a blood coagulation tube (BD vacutainer, USA) and centrifuged. Serum was isolated. The serum IGF-1 content was measured using the OCTEIA®Rat / Mouse IGF-1 kit (IDS; Immunodiagnostic systems, UK).

TABLE 3

Test Rest Growth Mature Hypertrophy  Control 48.7 ± 2.3 136.7 ± 6.1 153.1 ± 6.3 295.9 ± 5.2  Gogagapi and 6 kinds of herbal medicine 48.2 ± 2.6 116.8 ± 3.5 152.3 ± 4.8 285.9 ± 5.4  Ogapi and 6 Herbs, Bacillus Culture 45.3 ± 2.7 116.0 ± 3.2 183.2 ± 5.0 * 272.7 ± 5.4  Ogapi and 6 Herbs, Lactic Acid Bacteria Culture 53.9 ± 2.4 * 110.3 ± 4.3 162.6 ± 7.7 285.2 ± 7.2  Prickly Pear and 6 Herbs, Surfactants 43.4 ± 1.5 108.4 ± 2.8 157.7 ± 4.3 311.3 ± 6.4  Prickly Pear and Six Herbs, Surfactants, Lactic Acid Bacteria Culture, Bacillus Culture 47.9 ± 2.3 138.5 ± 6.9 159.3 ± 4.9 295.9 ± 5.2

*; p <0.05

< Experimental Example  2> Growth promotion efficacy test of existing and improved products

In each test group, SD rats of the same age and sex as Experimental Example 1 consisted of 5 normal weight mice (initial weight of 70 to 80 g or more), 3 low weight mice (initial weight of 30 to 40 g), and 8 in total. The test was divided into different cages for sufficient feed intake.

Existing products (trade name Kicker, fazin biosa, main ingredients-50% by weight of extracts of Sookji, acid, cornus, bokyeong, green and ginseng, 20% by weight lactose, 15% by weight seaweed calcium, cocoa powder 7% by weight, 5% by weight of xylitol, 2% by weight of chlorella, 1% by weight of brewer's yeast, 0.02% by weight of sucralose) and thorny scabies and six Chinese herbs were added to 100 mg / kg per kg of rat body weight, respectively. Next, 10 mg / kg of Bacillus culture was added to the first round of improvement, and 4 mg / kg and 0.1 mg / kg of vitamin AD3 and SFT-65 were respectively added to the second round of improvement, and the third round of the Bacillus culture and vitamin AD3 was added. , SFT-65 (polyethylene sorbitan monostearate ) was added to 10mg / kg, 4mg / kg, 0.1mg / kg, respectively, as shown in Table 4 below. As a result, the low-weight rats showed better feed intake and improved weight gain significantly (see FIG. 2 and Table 5).

TABLE 4

<Normal weight rat target>

Dosage (mg / kg) Long bone length (mm) Upper iliac width (mm) Long bone medium width (mm) Lower iliac width (mm) Iliac weight (mg)  Cod saline 100  29.98 ± 0.15  6.54 ± 0.07  2.41 ± 0.04  3.65 ± 0.05  318 ± 4.58 Kicker 100, Prickly Pear + 6 Herbs 100  30.45 ± 0.25  6.43 ± 0.09  2.47 ± 0.07  3.64 ± 0.06  324 ± 12.13 Kicker 100, Prickly Pear + 6 Herbs 100 Bacillus Cultures 10  30.16 ± 0.13  6.37 ± 0.03  2.42 ± 0.06  3.69 ± 0.03  328 ± 3.84 Kicker 100, Prickly Pear + 6 Herbs 100, AD3 4, SFT-65 0.1,   30.34 ± 0.09   6.38 ± 0.06   2.34 ± 0.04   3.54 ± 0.04   322 ± 6.41 Kicker 100, Viscera + 6 Herbs 100, Bacillus Culture 10, AD3 4, SFT-65 0.1   29.88 ± 0.15   6.22 ± 0.08   2.95 ± 0.04   3.53 ± 0.05   298 ± 7.03

TABLE 5

<Underweight rat subject>

Test Dosage (mg / kg) Long bone length (mm) Upper iliac width (mm) Long bone medium width (mm) Lower iliac width (mm) Iliac weight (mg)  Control  Saline  27.80 ± 0.12  6.13 ± 0.04  2.40 ± 0.06  3.70 ± 0.03  270 ± 4.82  existing Kicker 100, Prickly Pear + 6 Herbs 100  28.52 ± 0.31 *  6.16 ± 0.05  2.47 ± 0.06  3.56 ± 0.03  286 ± 4.87   Improvement 1 Kicker 100, Prickly Pear + 6 Herbs 100, Bacillus Cultures 10  28.33 ± 0.04 *  6.30 ± 0.09  2.32 ± 0.04  3.62 ± 0.06  279 ± 6.45   Improvement 2 Kicker 100, Viscera + 6 Herbs 100, AD3 4, SFT-65 0.1,   27.82 ± 0.15   6.05 ± 0.04   2.26 ± 0.06   3.35 ± 0.17   258 ± 4.83    Improvement 3 Kicker 100, Viscera + 6 Herbs 100, Bacillus Culture 10, AD3 4, SFT-65 0.1   28.46 ± 0.14 *   6.25 ± 0.09   2.45 ± 0.08    3.64 ± 0.03   287 ± 6.77 *

*; p <0.05

The test results of IGF-1 were measured that improvement 1 was most significantly higher in underweight mice (see Table 7, Figure 3).

TABLE 6

<Normal weight rat target>

Test Life IGF-1 (ng / ml) Control 1398 ± 115 existing 1415 ± 56 Improvement 1 1645 ± 59 * Improvement 2 1652 ± 45 * Improvement 3 1511 ± 48

*; p <0.05

TABLE 7

<Underweight rat subject>

Test Life IGF-1 (ng / ml) Control 1249 ± 47 existing 1272 ± 17 Improvement 1 1616 ± 55 ^* Improvement 2 1511 ± 67 ^* Improvement 3 1497 ± 18 ^*

*; p <0.05

The following is to prepare the present invention according to the dry form or liquid form.

< Example  1>

  50% by weight herbal extract extracted from a mixture comprising 75% by weight of barberry, 7% by weight of safflower seed, 5% by weight of Bokryeong, 3.25% by weight of mountain green, 3.25% by weight of green tea, 3.25% by weight of ginseng, and 3.25% by weight of cornus oil, lactose 20 Wt%, algae calcium 15%, cocoa powder 7% by weight, xylitol 5% by weight, Bacillus culture 3% by weight was mixed to complete the dry pharmaceutical composition having a growth promoting effect.

< Example  2>

  Herbal medicine extracted from a mixture comprising 50.5% by weight of Prickly Pear Extract, 14% by weight of Safflower Seed Extract, 10% by weight of Bokryeong Extract, 6.5% by weight Poach Extract, 6.5% by weight Green Extract, 6.5% by weight of Ginseng Extract, and 6% by weight of Cornus Extract 10% by weight of the extract, 0.5% by weight of Bacillus culture, 69% by weight of honey, 0.2% by weight of grapefruit seed extract, 0.3% by weight of apple flavor, 20% by weight of sodium carboxymethylcellulose, and then filled into a polypropylene resin packing liquid A pharmaceutical composition having a growth promoting effect in form was completed.

< Example  3>

  The composition of Example 1 is further mixed with 1% by weight of CMC-Na and 0.5% by weight of apple flavor, 0.3% by weight of vitamin C and then tableting with a tableting machine in a dry form having a growth promoting effect The composition of was completed.

By containing the growth active material of the present invention, the thorny opiaceae and the six herbal medicine extracts and Bacillus cultures have the effect that the growth promotion can be further improved, promote the growth during the growth stage of childhood-pubertal normal growth In addition, it has the effect of treating and preventing diseases related to growth disorders such as microcertification, dwarfism and poor growth and deterioration of children.

Claims (3)

It contains 10 to 90% by weight, 0.1 to 50% by weight of safflower seeds, 0.1 to 50% by weight, 0.1 to 50% by weight of cornus oil, 0.1 to 50% by weight of Fuling, 0.1 to 50% by weight of green rust, and 0.1 to 50% by weight of ginseng. 100 parts by weight of the herbal extract extracted from the mixture to be; Bacillus subtilis is characterized by excellent starch resolution, excellent protein resolution, and breaks down carbohydrates of fats and polymers to produce low molecular weight amino acids, nucleic acids, fatty acids, vitamins, alkaloids, and viscous polymers. Bacillus subtilis MORI KCCM 10450 was inoculated in a medium containing 2% by weight of corn starch, 1.0% by weight of soybean meal, 0.5% by weight of yeast extract, 0.1% by weight of potassium monophosphate, 0.02% by weight of antifoaming agent and pH 7.5 After culturing for 3 days at 250rpm, 37 ~ 38 ℃, aeration rate 0.3vvm, calcium carbonate was added at 10% of the culture medium weight, and then stirred at inlet temperature of 120 ℃ and outlet temperature of 85 ℃. 0.1 to 10 parts by weight of the culture of Bacillus subtilis S. aureus obtained by spray drying with; Lactobacillus helveticus ( Lactobacillus helveticus ) bacteria 1% by weight, meat extract 1%, yeast extract 0.5%, glucose 2% by weight, sorbitan monooleate 0.01% by weight, ammonium citrate 0.5%, sodium acetate Inoculated in a medium containing 0.01% by weight, 0.01% by weight of magnesium sulfate, 0.005% by weight of manganese sulfate, 0.2% by weight of potassium diphosphate, and incubated at 120 rpm for 3 days at 37 ° C, followed by centrifugation at 8,000 rpm. 5 parts by weight of a culture of Lactobacillus helvetticus obtained by only adding Lactobacillus helvetticus and then adding a skim milk (skim milk) corresponding to 30% of the original weight; And 5 parts by weight of a surfactant. delete delete

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