KR100861943B1 - Fish feed and its use for simultaneously improving the growth rate of fish while converting conjugated linoleic acid to fish lipid components - Google Patents

Abstract

The present invention is a fish feed containing carotenoids, DHA (docosahexaenoic acid), EPA (eicosapentaenoic acid), CLA (conjugated linoleic acid) and anti-cancer, antioxidant Provides methods of producing functional fish such as sex.

According to the present invention, as a result of adding the conjugated linoleic acid, natural carotenoid pigment and polyunsaturated fatty acid to the fish feed, it was possible to overcome the problem that growth is inhibited when the conjugated linoleic acid is ingested in large quantities, Oxidation stability can be extended to improve storage stability, antioxidant and anticancer effects, and body fat reduction effect, resulting in functional lipids and fish oils.

Description

Feed for fishes for conversion CLA into lipid components of fish and for improvement of growth ratio of fishes, and the use }

The present invention relates to a feed for fish and its use for simultaneously converting conjugated linoleic acid to a fish lipid component and improving the growth rate of the fish, producing a feed containing a certain amount of carotenoid pigment, polyunsaturated fatty acid and conjugated linoleic acid The present invention relates to a fish obtained by feeding the feed and extracting and purifying functional lipids from the fish.

The carotenoid pigment is a pigment contained in plants and marine organisms and is known as a physiologically active substance that exhibits antioxidant, anticancer, antimutagenic and coloring effects on the color of salmon and fish.

In addition, the polyunsaturated fatty acid may include well-known DHA and EPA, and helps improve cholesterol, smooth circulation of blood, and supply of brain nutrition. The conjugated linoleic acid may be anti-cancer, antioxidant, anti-cholesterol, It is known as a natural multifunctional fatty acid that has antifungal and animal body fat reduction effects.

On the other hand, the conventional techniques for the functional feed or fish in relation to the carotenoid pigment is a manufacturing method of artificial coloring agent for salmon meat coloring using the shell color (10-1992-0001015), separated from the dietary fiber separated from the seafood shell Purification Method and Preparation of Functional Food Added thereto (10-1999-0043469), Production Method of Cellulose Using Snail Shell (10-1999-0043944), Feed Composition for Laying Hens for Improving Coloration and Eggshell Quality Anti-thrombotic composition comprising special egg (10-2000-0012290) prepared using the feed composition, anti-cholesterol composition (10-2001-0025097) containing acetone extract of squirrel bark, and ethyl acetate extract of squirrel bark ( 10-2001-0030716) and the like are known.

In addition, the related art related to conjugated linoleic acid is a method for producing functional eggs using conjugated linoleic acid-containing feed (10-1996-0044806), high purity conjugated linoleic acid isomer separation method (10-2000-0022905), airspace Novel strains for producing linolenic acid, capsules containing them and functional foods (10-2005-0004128), feed containing conjugated linoleic acid, methods for producing functional eggs, chicken and fish (10-1996) -0044806, and the like.

However, as mentioned in the above-mentioned prior arts, when a large amount of lipids such as CLA is added to the fish feed, there is a problem in that the growth rate of the fish is lowered due to the high content of fat oxide in the fish feed. In other words, conjugated linoleic acid is known to prevent cancer by ingesting about 3g / 60kg / day as an adult. However, when conjugated linoleic acid is ingested in fish at least 1% of feed additives, the growth of fish is inhibited. There was a problem.

Accordingly, the present inventors pay attention to the fact that in providing a fish feed to solve the above-mentioned problems, it is possible to provide a functional lipid showing storage stability if the lipid component such as CLA can be changed into a component of fish lipid. It was completed.

In other words, an object of the present invention is to change the lipid component to a component of fish lipids by providing a feed containing carotenoids and conjugated linoleic acid, which inhibits oxidation of polyunsaturated fatty acids and provides antioxidant and anticancer effects in providing fish feed. To provide a functional lipid that exhibits storage stability, antioxidant and anticancer effects, and body fat reduction effect.

Another object of the present invention is to produce a functional fish by feeding a fish feed obtained according to the above-described method.

Another object of the present invention is to obtain functional lipids from functional fish.

 In order to achieve the above object, the present invention provides a feed for fish comprising a carotenoid, polyunsaturated fatty acid and conjugated linoleic acid contained in fish oil.

The present invention also provides a method of producing functional fish by feeding the obtained fish feed.

Furthermore, the present invention provides functional lipids from functional fish.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The fish feed of the present invention comprises a carotenoid, polyunsaturated fatty acid and conjugated linoleic acid in addition to a conventional fish feed consisting of fish meal, soybean meal, wheat flour, vitamins, minerals, soybean oil and the like.

The carotenoids may be added within 0.5% by weight or less based on the total weight of the fish feed. This is an effective range for promoting the growth of fish, and exceeding this is not preferable because the growth promoting effect is poor. Moreover, as the kind, both synthetic carotenoids or natural carotenoids can be used, and it is more preferable to use the natural pigment extracted from the off-white shell etc. especially.

The polyunsaturated fatty acid is preferably added within the range of 5 to 25% by weight based on the total weight of the fish feed. This is not preferable because it is a range effective to promote the growth of fish, the growth promoting effect is poor beyond this. Examples thereof include, but are not limited to, DHA or EPA, and all of them are preferably extracted from fish.

In addition, the conjugated linoleic acid is preferably added within the range of 5% by weight or less based on the total weight of the fish feed. Most feed-added experiments show that fish fed 5% or more conjugated linoleic acid significantly reduced fish growth. In addition, the kind is not limited as long as it is available, and what synthesize | combined from fats and oils is especially preferable.

The fish feed thus obtained is fed to produce functional fish. At this time, the amount of salary twice a day, 3% by weight of the fish is enough for about 8 weeks.

As described above, functional lipids can be obtained from functional fish fed by feeding fish feed.

By using the obtained functional lipids can be obtained functional fish meat and food with improved storage stability, wherein the form of the functional food includes the form of capsules, tablets, granules and the like. In addition, the lipid obtained may be lyophilized to be used in powder form.

On the other hand, functional fish oil can be obtained from the intestines of functional fish fed and fed fish feed.

According to the above, the addition of conjugated linoleic acid, natural carotenoid pigment and polyunsaturated fatty acid to fish feed resulted in overcoming the problem that growth is inhibited when a large amount of conjugated linoleic acid is ingested, thereby prolonging the oxidative stability of fish meat. The storage stability was improved, and the accumulation of carotenoid pigments had the effect of improving fish quality. Accumulation of DHA and EPA can improve blood circulation and prevent coronary artery disease, and conjugated linoleic acid can reduce the weight loss. It was shown to be effective. As a result, functional lipids and fish oils can be obtained.

Hereinafter, the configuration and operation of the present invention through the examples will be described in more detail. Each example was repeated to confirm the reproducibility of the results.

  <Example 1> carotenoid, polyunsaturated fatty acid and CLA blending ratio

In order to determine the optimal ratio of lipid components in the feed, 45% of fish meal on the market, 7% of soybean meal, 25% of wheat flour to give viscosity, 2% of vitamin mixture and 1% of mineral mixture to facilitate metabolism The diet was added as a basic feed, and squid liver oil containing polyunsaturated fatty acid 6.4% and soybean oil 13.6% were added as a control diet.

To the carotenoid and conjugated linoleic acid (hereinafter referred to as "CLA")-containing feed composition according to the present invention, carotenoids containing 0.2% of synthetic carotenoids and 0.4% of natural carotenoids extracted from off-white shells were added to the above-mentioned composition, respectively. CLA was adjusted so that the ratio of soybean oil was 1% and 5%.

Feed composition and ingredient content are shown in Table 1 below. The control group was a sphere without addition of the conjugated linoleic acid and the pigment, Test Example 1 is a conjugated linoleic acid 1% and caroten pink 0.2%, Test Example 2 is a conjugated linoleic acid 1% and natural carotenoid 0.4%, Test Example 3 Silver conjugated linoleic acid 5% and caroten pink 0.2%, Test Example 4 prepared a test feed added with conjugated linoleic acid 5% and natural carotenoid 0.4%, and analyzed the general components of these feeds and the results It is shown together in Table 1.

Test Control Test Example 1 Test Example 2 Test Example 3 Test Example 4 Feed mixture (g / kg) Fishmeal 450 450 450 450 450 Soybean meal 70 70 70 70 70 flour 250 250 250 250 250 Vitamin Mix 20 20 20 20 20 Mineral mixtures 10 10 10 10 10 Squid Liver Oil 64 64 64 64 64 Soybean oil 136 124 122 84 82 Conjugated linoleic acid (CLA) 0 10 10 50 50 Pigment   Carofil Pink 0 2 0 2 0   Sea urchin extract 0 0 4 0 4 General ingredient (%)   Building quantity 93.0 ± 0.2 92.7 ± 0.1 92.5 ± 0.1 92.5 ± 0.1 92.2 ± 0.1   Crude protein 50.7 ± 2.1 51.0 ± 2.4 50.7 ± 1.3 50.3 ± 1.9 51.5 ± 1.2   Crude fat 20.4 ± 1.5 20.5 ± 1.1 20.9 ± 0.4 20.5 ± 1.2 19.3 ± 0.3   Ash  9.5 ± 0.0  9.6 ± 0.0  9.7 ± 0.0  9.5 ± 0.1  9.6 ± 0.1

As shown in Table 1, the dry matter content of both control and test diets was 92.2-93.0%, the crude protein content was 50.3-51.5%, the crude fat content was 19.3-20.9%, and the ash content was 9.5-9.7%. Feed composition ratio was shown.

  Example 2 Fatty Acid Composition of Feed

  The feed should contain nutrients that directly affect the growth of the fish, of which the lipid component is the lipid component from each of the feeds prepared in Example 1, considering that it is the most important energy component of the fish feed. Was extracted and the identification of these fatty acids was carried out according to the Food Code, the main fatty acid composition results obtained are summarized in Table 2 below.

fatty acid(%) Control Test Example 1 Test Example 2 Test Example 3 Test Example 4 16: 0 13.7 ± 0.2 13.7 ± 0.1 13.7 ± 0.1 11.8 ± 0.5 11.4 ± 0.1 18: 0 3.9 ± 0.1 3.7 ± 0.2 3.8 ± 0.1 2.9 ± 0.1 2.9 ± 0.1 ΣSaturates 20.3 ± 0.9 20.2 ± 0.8 20.3 ± 0.5 17.6 ± 0.2 16.2 ± 0.4 18: 1n-9 19.2 ± 1.0 19.2 ± 0.5 19.4 ± 0.2 21.6 ± 0.7 22.9 ± 0.4 18: 1n-7 1.8 ± 0.1 1.7 ± 0.1 1.7 ± 0.2 1.4 ± 0.1 1.6 ± 0.2 ΣMonoenes 24.7 ± 0.8 24.0 ± 1.1 24.2 ± 0.8 25.5 ± 0.5 27.1 ± 0.6 18: 2n-6 40.3 ± 3.2 34.5 ± 2.4 35.8 ± 2.2 17.0 ± 1.4 17.4 ± 1.0 18: 3n-3 5.2 ± 0.1 4.2 ± 0.1 4.4 ± 0.1 2.0 ± 0.2 2.1 ± 0.1 20: 4n-6 0.3 ± 0.1 0.3 ± 0.1 0.4 ± 0.0 0.9 ± 0.1 0.2 ± 0.0 20: 4n-3 0.2 ± 0.0 0.2 ± 0.0 0.2 ± 0.0 0.2 ± 0.0 0.1 ± 0.0 20: 5n-3 (EPA) 3.2 ± 0.2 3.3 ± 0.1 3.4 ± 0.2 3.1 ± 0.2 2.0 ± 0.1 22: 6n-3 (DHA) 3.2 ± 0.1 3.5 ± 0.5 3.4 ± 0.1 3.2 ± 0.1 2.8 ± 0.1 ΣPolyenes 54.7 ± 2.9 50.8 ± 2.5 51.7 ± 2.2 28.0 ± 3.0 25.5 ± 2.4 Total CLA 0.0 ± 0.0 4.7 ± 0.6 3.5 ± 0.4 28.6 ± 1.9 30.9 ± 1.6 Σn-6    40.5    34.6    35.9    17.1    17.5 Σn-3    12.4    11.9    12.0     9.1     7.4 n-6 / n-3     3.3     2.9     3.0     1.9     2.4

As shown in Table 2, the content of saturated fatty acids (saturates) was 20.3% in the control, the lowest in Test Example 4 16.2% added 0.5% natural carotenoids, Test Examples 1 and 2 were 20.2%, 20.3%, respectively It was.

Monoenoic acid (monoenes) was the highest in the control group 24.7%, Test Example 4 27.1%. The content of linoleic acid (18: 2n-6), an essential fatty acid of rainbow trout, was 17.0% and 17.4% for 5% CLA and 17.0% for CLA, respectively. 2 was 34.5% and 35.8%, respectively, and the control was 40.3%.

In contrast, the contents of CLA were the highest in Test Examples 3 and 4 (28.6% and 30.9%, respectively), and Test Examples 1 and 2 contained 4.7% and 3.5%, respectively, in appropriate proportions. The content of polyenes) is considered to be appropriate.

In addition, the ratio of n-6 / n-3 for evaluating the ratio of essential fatty acids in rainbow trout is known to be 2-4. Considering this, the n-6 / n-3 ratio of each feed used in the experiment It is considered to be properly manufactured in the range of 1.9 to 3.3.

  Example 3 Growth and Growth Index of Rainbow Trout

  The fish used in the experiment was about 150g of rainbow trout, which can simultaneously metabolize natural carotenoid pigment, polyunsaturated fatty acid, and CLA. 10 fish per experiment were placed in a 1-ton tank at 10 am and 6 pm 2 per day. Sashimi was fed at 3% of body weight and raised for 8 weeks.

The water quality of the breeding tank was designed to be circulated by the circulating filtration system, and the average water temperature was measured in growth weights of rainbow trout while maintaining the average of 11.0 to 17.2 ° C.

Test Control Test Example 1 Test Example 2 Test Example 3 Test Example 4  Body weight before experiment (g) 153.6 ± 13.3 153.2 ± 13.7 154.1 ± 12.4 155.2 ± 15.4 153.8 ± 14.8  Body weight after experiment (g) 421.0 ± 67.2 422.6 ± 66.4 471.4 ± 67.0 404.4 ± 51.8 411.3 ± 57.7  Daily growth rate    3.80    3.84    4.32    3.61    3.71  Feed efficiency    0.73    0.73    0.76    0.71    0.74

As shown in Table 3, Test Examples 3 and 4, which were added 5% CLA after 8 weeks, showed the slowest growth to 404.4g and 411.3g, respectively, and Test Example 1, which was added 1% CLA, weighed 422.6 g and feed efficiency 0.73. Although similar to the control, Test Example 2 weight 471.4g, feed efficiency 0.76 was higher than the control, the rainbow trout CLA content of less than 5% by weight, natural pigment less than 5% by weight was found to be appropriate there was.
Example 4 Carotenoid Content in Rainbow Trout Muscle and Shell

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  The quality of rainbow trout is determined by the degree of red pigmentation in the muscle. Therefore, in the case of aquaculture, a certain amount of pigment is added to the feed, but the pigment content according to the addition of natural carotenoid pigments, not synthetic dyes, is analyzed to meet the needs of consumers seeking well-being and the results are summarized in Table 4 below. .

Carotenoid content in each site (mg / kg) Test Control Test Example 1 Test Example 2 Test Example 3 Test Example 4 Muscle 4 weeks after experiment  1.9 ± 0.0  6.9 ± 0.1  7.8 ± 0.0  8.0 ± 0.1  8.8 ± 0.1 4 weeks after experiment 13.2 ± 2.3 55.3 ± 2.3 40.3 ± 1.6 53.7 ± 2.1 43.1 ± 3.0 Muscle after 8 weeks of experiment  2.3 ± 0.0  13.8 ± 1.6 10.2 ± 0.4 9.7 ± 1.2 9.5 ± 0.9 8 weeks after experiment 14.0 ± 2.2 69.3 ± 3.6 53.0 ± 4.4 63.7 ± 4.2 58.7 ± 3.6

 As shown in Table 4, the carotenoid content was 1.9 mg / kg in the control, 6.9 and 7.8 mg / kg in Test Examples 1 and 2, 8.0 and 8.8 mg / kg in Test Examples 3 and 4 after 4 weeks of experiment. , After 8 weeks of experiment, the control group showed the highest accumulation in 2.3 mg / kg, Test Examples 1 and 2, 13.8 and 10.2 mg / kg in Test Examples 1 and 2.

  Example 5 Lipid Class Composition of Rainbow Trout Muscle and Visceral Lipids

In the above-described Example, the muscle and visceral lipids of Test Example 2, which had the best growth and carotenoid accumulation after 8 weeks of culture, were extracted and the lipid class composition was examined.
Specifically, fish meat and intestines were separated from the reared fish, and finely cut, and 1 g of chloroform and 2 times of alcohol were added to about 100 g of the sample, and homogenized with homogenization at 16,000 rpm for about 3 minutes. Homogenized samples were prepared in Toyo No. Filtration with 5 filter paper separated the filtrate and residue. Put the same amount of chloroform in the filter paper together with the residue, homogenize for 2 minutes, combine the filtrate obtained by filtration with the first separated filtrate and transfer to the separation filter, add 0.88% KCl corresponding to 1 times of the sample, 4 ~ 10 ℃ After standing for one day at, the water layer and the chloroform layer were separated. Put the chloroform layer into the Erlenmeyer flask and remove 2 ~ 5g of anhydrous sodium sulfate dried for more than 5 hours in a dryer to remove the excess water contained in the extract, leave for 4 minutes at 4 ~ 10 ℃, and then filter only the chloroform layer and vacuum Concentrated under reduced pressure at 40 ℃ or less using an evaporator to obtain a lipid.
2) Fatty Acid Methyl Ester Derivatization
Extracted from fish 50 mg of concentrated lipid and 1 mL of internal standard (C _{23: 0} methyl ester) (1 mg C _{23: 0} ) were taken in a cap tube, and 1.5 mL of 0.5 N NaOH-methanol solution was added to nitrogen and charged at 100 ° C. It was heated for 3 minutes and saponified. After cooling, 2 mL of 1.0 N sulfuric acid was added, followed by nitrogen filling. The tube was tightly tightened, and heated at 55 ° C. for 30 minutes to be methylated. After cooling to about 30 ~ 40 ℃ 1mL isooctane was added, filled with nitrogen and mixed with a shake mixer for 30 seconds. Immediately 5 mL of saturated saline was added, filled with nitrogen and shaken to separate the isooctane layer. After transferring the isooctane layer to the sample bottle, 1 mL of isooctane was added again, and then shaken and reextracted to collect a sample bottle, which was used as a fatty acid methyl ester sample.
3) Gas liquid chromatography (GLC)
GLC used for fatty acid analysis was performed using Shimadzu GC 14A with Omegawax-320 fused silica capillary coulmn (30mx 0.32mm, id, SUPELCO, supelco Park, PA, USA). Analytical conditions were a column temperature of 185-230 ° C. (3 ° C./min), an inlet temperature of 250 ° C., and a detector temperature of 250 ° C., and a transfer gas of He (1.0 kg / cm 3) was used. The analysis of fatty acids was identified by comparison with the standards analyzed under the same conditions. The fatty acid standards were 14: 0, 16: 0, 18: 0, 18: 1, 18: 2, 18: 3, 20: 0, 22: 0, 22: 1, 24: 0 (Sigma) was used.
The lipid class composition thus obtained was analyzed to evaluate the nutrition of the fed feed lipids, and the results obtained are summarized in Table 5 below.

 Geological class (%) Control Test Example 1 Test Example 2 Test Example 3 Test Example 4                       Muscle (8 weeks)  Free fatty acids trace trace trace trace trace  Triacylglycerol 73.8 ± 5.9 74.1 ± 3.2 78.9 ± 4.0 75.5 ± 6.4 79.2 ± 0.9  Sterol  1.7 ± 0.3  1.8 ± 0.2  2.3 ± 0.9  2.2 ± 0.4  1.8 ± 0.3  Polar lipids 24.5 ± 5.6 24.1 ± 3.4 18.8 ± 2.6 22.3 ± 2.7 19.0 ± 0.8                       Built-in (8 weeks)  Free fatty acids trace trace trace trace trace  Triacylglycerol 78.5 ± 3.3 84.5 ± 4.6 89.0 ± 4.1 80.1 ± 3.1 84.1 ± 2.8  Sterol  0.9 ± 0.1  1.2 ± 0.0  0.9 ± 0.3  8.6 ± 2.1  5.8 ± 1.8  Polar lipids 20.7 ± 3.4 14.3 ± 4.6 10.1 ± 3.9 11.2 ± 2.8 10.1 ± 2.9

As shown in Table 5, free fatty acids did not accumulate in the fish body after feeding the prepared feed, and triacylglycerol occupied most of the muscle fat.

After 8 weeks, triacylglycerols were 74.1 and 78.9%, sterols were 1.8 and 2.3%, polar lipids were 24.1 and 18.8%, respectively. 84.5 and 89.0% of acylglycerols, 1.2 and 0.9% of sterols, 14.3 and 10.1% of polar lipids, further accumulating triacylglycerol in the intestines.

 Example 6 Fatty Acid Composition of Rainbow Trout Muscle after 8 Weeks of Breeding

  Since the lipid contained in the feed is reflected in the fish, the change in fatty acid composition which directly affects the growth of rainbow trout after 8 weeks was analyzed in the same manner as in Example 5, and the results are summarized in Table 6 below.

fatty acid Control Test Example 1 Test Example 2 Test Example 3 Test Example 4 16: 0 14.8 ± 1.1 14.4 ± 1.1 14.9 ± 0.8 14.6 ± 0.9 13.9 ± 0.1 18: 0 4.2 ± 0.0 4.9 ± 0.6 4.7 ± 0.2 4.4 ± 0.1 3.9 ± 0.1 ΣSaturates 22.5 ± 0.3 22.5 ± 0.3 23.0 ± 0.2 22.6 ± 0.2 21.3 ± 0.0 18: 1n-9 20.3 ± 1.1 21.1 ± 1.0 20.1 ± 0.7 21.6 ± 0.5 22.3 ± 0.4 18: 1n-7 2.4 ± 0.2 2.3 ± 0.1 2.6 ± 0.1 2.3 ± 0.2 2.4 ± 0.0 ΣMonoenes 29.7 ± 0.2 30.4 ± 0.2 29.5 ± 0.1 32.0 ± 0.2 33.1 ± 0.1 18: 2n-6 26.0 ± 1.9 24.0 ± 1.2 25.7 ± 1.6 16.8 ± 0.4 17.7 ± 0.2 18: 3n-3 2.9 ± 0.3 2.9 ± 0.2 2.7 ± 0.1 1.8 ± 0.1 1.9 ± 0.0 20: 4n-6 0.7 ± 0.1 0.6 ± 0.2 0.7 ± 0.0 0.6 ± 0.1 0.7 ± 0.0 20: 4n-3 0.6 ± 0.1 0.7 ± 0.2 0.6 ± 0.2 0.5 ± 0.2 0.6 ± 0.0 20: 5n-3 (EPA) 2.5 ± 0.3 2.5 ± 0.3 2.2 ± 0.2 2.5 ± 0.3 2.2 ± 0.0 22: 6n-3 (DHA) 7.5 ± 0.6 7.9 ± 1.1 7.2 ± 0.4 7.0 ± 0.5 7.8 ± 0.3 ΣPolyenes 47.7 ± 0.2 45.1 ± 0.2 45.1 ± 0.1 34.7 ± 0.2 36.5 ± 0.1 Total CLA 0.0 ± 0.0 2.0 ± 0.1 2.2 ± 0.1 10.6 ± 0.9 9.1 ± 0.3 Σn-6    29.0    27.0    28.6    19.0    20.2 Σn-3    15.7    16.3    14.7    14.0    14.6 n-6 / n-3     1.8     1.7     1.9     1.4     1.4

As shown in Table 6, the polyenoic acid in each test zone of muscle lipid was 34.7 ~ 47.7%, the conjugated linoleic acid content of Test Examples 1 and 2 is 2.0 and 2.2%, and the conjugated linoleic acid content of Test Examples 3 and 4 is Accumulation was increased in the group fed the feed with a high addition amount of 10.6 and 9.1%, but n-6 / n-3, which is the nutritional evaluation index of Test Examples 3 and 4, was 1.4, which was less than the proper amount.

The content of 18: 3n-3, an essential fatty acid in rainbow trout, was also found to be appropriate.

Example 7 Fatty Acid Composition of Defensive Muscle After 8 Weeks of Breeding

  Defense is a fish that grows in seawater and contains a large amount of lipids in muscle, which is suitable for assessing changes in lipids in feed. Fatty acid composition changes directly affecting the growth of defense during 8 weeks of breeding were analyzed in the same manner as in Example 5 and the results are summarized in Table 7 below.

fatty acid Control Test Example 1 Test Example 2 Test Example 3 Test Example 4 16: 0 15.2 ± 0.9 16.5 ± 1.5 16.6 ± 0.7 15.4 ± 0.6 16.6 ± 0.4 18: 0 6.9 ± 0.6 6.1 ± 0.4 4.6 ± 0.0 4.3 ± 0.1 4.6 ± 0.1 ΣSaturates 25.2 ± 0.3 27.2 ± 0.3 24.7 ± 0.1 25.0 ± 0.1 25.3 ± 0.1 18: 1n-9 11.1 ± 3.3 13.9 ± 2.2 14.6 ± 0.3 16.7 ± 0.9 17.7 ± 0.3 18: 1n-7 2.7 ± 0.5 3.0 ± 0.3 4.4 ± 0.1 4.6 ± 0.2 4.6 ± 0.1 ΣMonoenes 19.8 ± 0.6 24.4 ± 0.4 29.0 ± 0.1 30.6 ± 0.3 31.6 ± 0.1 18: 2n-6 12.9 ± 0.8 12.4 ± 0.5 8.5 ± 1.0 9.5 ± 0.2 6.2 ± 0.7 18: 3n-3 1.1 ± 0.0 0.6 ± 0.2 1.2 ± 0.1 0.7 ± 0.0 0.6 ± 0.1 20: 4n-6 1.6 ± 0.0 0.6 ± 0.2 0.5 ± 0.0 0.8 ± 0.1 1.5 ± 0.1 20: 4n-3 0.4 ± 0.1 0.3 ± 0.2 0.3 ± 0.1 0.3 ± 0.2 0.3 ± 0.1 20: 5n-3 (EPA) 6.6 ± 0.4 5.1 ± 0.5 5.7 ± 0.1 5.6 ± 0.2 5.7 ± 0.2 22: 6n-3 (DHA) 19.4 ± 1.1 9.4 ± 0.9 10.2 ± 0.6 6.5 ± 1.1 10.2 ± 1.7 ΣPolyenes 52.5 ± 0.1 34.6 ± 0.2 33.5 ± 0.1 26.3 ± 0.2 26.3 ± 0.2 Total CLA 0.0 ± 0.0 14.4 ± 0.0 13.8 ± 0.1 18.0 ± 0.5 19.4 ± 0.2 Σn-6    15.4    13.3     9.1    10.5     7.9 Σn-3    32.5    16.0    17.2    14.5    17.8 n-6 / n-3     2.1     1.9     0.5     0.7     0.5

As shown in Table 7, the CLA-added polyenoic acid in the defense muscle was 26.3-34.6%, the content of conjugated linolenic acid was 14.4% and 13.8% in Test Examples 1 and 2, respectively, 18.0% in Test Examples 3 and 4, 19.4% showed a difference in the accumulation amount according to fish species, and CLA accumulation was high in marine fish.

The ratio of n-6 / n-3 was 0.5 ~ 1.9 in the conjugated linolenic acid addition groups, respectively, which was lower as the amount of CLA added.

Therefore, in order to obtain a large amount of functional lipids, it is considered effective to use sea fish as a sample.

  Example 8 Radical Scavenging Activity of Lipids Extracted from Breeding Rainbow Trout

  In this example, the antioxidant power through DPPH radical scavenging ability was measured in order to measure the change in the functional lipid component of the rainbow trout according to the present invention.

At this time, in order to extract the lipid of the radical scavenging activity experiment, 1-fold chloroform and 2-fold alcohol were added to about 100 g of rainbow trout meat and internal organs, and homogenized with homogenization at 16,000 rpm for about 3 minutes. Homogenized samples were prepared in Toyo No. Filtration with 5 filter paper separated the filtrate and residue. Put the same amount of chloroform in the filter paper together with the residue, homogenize for 2 minutes, combine the filtrate obtained by filtration with the first separated filtrate and transfer to the separation filter, add 0.88% KCl corresponding to 1 times of the sample, 4 ~ 10 ℃ After standing for one day at, the water layer and the chloroform layer were separated. The same amount of distilled water was added to the chloroform layer remaining in the separating funnel, mixed and separated into a chloroform layer and a water layer.Then, the chloroform layer was placed in an Erlenmeyer flask and dried in a drier for at least 5 hours to remove excess moisture contained in the extract. 2-5 g of anhydrous sodium sulfate was added, and the mixture was left for 4 minutes at 4 ° C to 10 ° C, and then the chloroform layer was filtered and concentrated under reduced pressure at 40 ° C or below using a vacuum evaporator to obtain lipids.
In order to use this lipid extract as a sample, a concentrate was completely removed from the solvent by a rotary evaporator.
Then, the concentrate was dissolved in 10 mg / mL DMSO (dimethyl sulfoxide), 10, 25, 50, and 100 μg / mL of the solution were mixed with 2 mL of 0.0005M DPPH, and reacted at room temperature for 30 minutes, and then the reaction was absorbed at 517 nm. The radical scavenging ability of the lipid component of the rainbow trout obtained as a result of the measurement is summarized in Table 8 below.

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Concentration (μg / mL) DPPH radical scavenging activity (μg / mL) muscle guts Ascorbic acid Tocopherol 10 trace trace 95.5 ± 0.6 19.1 ± 0.5 25 trace trace 95.8 ± 0.4 55.3 ± 0.7 50 11.7 ± 0.4 11.3 ± 0.7 96.1 ± 0.9 82.3 ± 0.6 100 22.6 ± 0.2 24.9 ± 0.5 96.6 ± 1.3 93.2 ± 0.6

As shown in Table 8, the ascorbic acid used as a control showed an inhibitory effect of at least 95% regardless of the concentration, tocopherol 19% at 10μg / mL concentration, 82% at 50μg / mL concentration, At 100 μg / mL, the inhibitory effect was over 93%.

However, rainbow trout muscle and viscera extract showed more than 20% inhibitory effect at the concentration of 100μg / mL and can be expected to be about 1,000 times the effect when converted to about 10g of lipid that can be ingested from a rainbow trout.

 Example 9 Storage Stability of Lipids Extracted from Breeding Rainbow Trout

  To 60 mg of the concentrate used for the radical scavenging ability was mixed 125 mg of linoleic acid.

0.7 mL 5 mM ferric chloride, 0.2 mL 8.1% sodium dodecylsulfate, 1.5 mL 20% acetic acid (pH 3.5), 1.5 mL 0.8% thiobarbituric acid in 0.1 mL of the mixed solution acid), 50 μL 0.8% Bechti (BHT) were added in this order and then heated at 5 ° C. for 60 minutes and at 100 ° C. for 60 minutes.

Subsequently, 1.0 mL of deionized water was added and red pigment was extracted with a 5.0 mL normal-butylol / pyridine (15: 1) mixed solution to measure the degree of oxidation at 532 nm. The results are summarized in Table 9 below. .

Storage period (days) Storage stability (TBARS, nmol / mL) muscle guts Linoleic acid Tocopherol 0 98.9 ± 1.3 99.1 ± 2.6 95.5 ± 0.6 99.7 ± 0.5 One 95.4 ± 2.5 95.6 ± 3.7 11.7 ± 0.4 95.5 ± 1.3 2 87.2 ± 3.2 91.4 ± 2.4 95.8 ± 0.4 91.9 ± 3.1 3 85.2 ± 3.4 81.3 ± 1.8 96.1 ± 0.9 84.3 ± 2.0 4 62.7 ± 2.2 53.1 ± 2.5 11.7 ± 0.4 72.8 ± 2.6 5 58.6 ± 1.9 49.8 ± 2.2 11.7 ± 0.4 70.0 ± 1.5 6 19.9 ± 1.6 20.3 ± 1.7 11.7 ± 0.4 57.7 ± 2.1

As shown in Table 9, the tocophenol used as a control showed a stability of 99.7% at the beginning of the experiment, and gradually began to oxidize from the first day of storage, followed by 84% on the third day of storage, and 57% from the sixth day of storage. It appeared to progress rapidly.

In case of rainbow trout meat, it began to decrease to 87% from the second day of storage, and began to oxidize to 58% on the fifth day of storage. It was.

It has been shown that the storage period can be extended when the feed was raised.

Therefore, the addition of CLA, natural carotenoid pigment and polyunsaturated fatty acid to fish feed resulted in prolonged storage period and prolonged the oxidative stability of fish meat.

Preparation Example 1 Preparation of Functional Lipid-Containing Fish Meat Powder

  Only fish meat was recovered from the fish containing the functional lipid obtained in Example 3, and a fish meat powder having a water content of 5% or less was prepared using a vacuum freeze dryer.

 Preparation Example 2 Preparation of Functional Lipid-Containing Functional Fish Oil

  A functional lipid-containing oil was obtained by using an organic solvent such as alcohol of 3 to 10 times in the intestines of the fish containing the functional lipid obtained in Example 5.

 Preparation Example 3 Preparation of Functional Lipid-Containing Functional Food

  1 to 100% of the functional lipid-containing fish oil obtained in Preparation Example 2 was added to obtain functional capsules, tablets, granules, and the like by a conventional manufacturing method.

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According to the above, the addition of conjugated linoleic acid, natural carotenoid pigment and polyunsaturated fatty acid to fish feed resulted in overcoming the problem that growth is inhibited when a large amount of conjugated linoleic acid is ingested, thereby prolonging the oxidative stability of fish meat. The storage stability was improved, and the accumulation of carotenoid pigments had the effect of improving fish quality. Accumulation of DHA and EPA can improve blood circulation and prevent coronary artery disease, and conjugated linoleic acid can reduce the weight loss. It was shown to be effective. As a result, functional lipids and fish oils can be obtained.

Claims (16)

In the fish feed consisting of fish meal, soybean meal, wheat flour, squid liver oil, vitamins, minerals, soybean oil, Based on the total weight of the fish feed, 0.5% by weight of carotenoids selected from at least one of carotenoids extracted from natural carotenoids and synthetic carophil pink; 5-25% by weight polyunsaturated fatty acid extracted from fish, and 1-5% by weight of conjugated linoleic acid, comprising as an active ingredient, fish feed for improving the growth rate of fish at the same time while converting conjugated linoleic acid to fish lipid components. delete delete delete delete delete delete delete delete Feed the fish feed of claim 1 to the fish, Separating viscera from fish with improved growth rate by converting conjugated linoleic acid in the fish feed to fish lipid components, and Fish oil is obtained by adding 3-10 times alcohol based on the weight of the intestines to the intestines, fish oil production method. Feed the fish feed of claim 1 to the fish, Separating fish meat from fish with improved growth rate while converting conjugated linoleic acid in the fish feed into fish lipid components, and Vacuum freezing the separated fish meat obtained in the form of a powder having a water content of less than 5%, a method for producing fish meat powder. delete delete delete delete delete