KR100721983B1 - Method of Extracting Finitol from Non-Repair and Functional Food Using Extract - Google Patents

Abstract

The present invention relates to a method for manufacturing a blood glucose control functional food using natural pinitol, which is useful for diabetes, by extracting pinitol, a blood sugar control substance, from a non-repair, in which a non-repair is added to ethanol. Additive grinding step of grinding with a homogenizer, precipitation step of leaving the crushed non-repair in alcohol, centrifugation step of centrifugation of the supernatant recovered from the precipitation step, and drying under reduced pressure to dry the supernatant liquid recovered from the centrifugation step under reduced pressure. In addition, a separation step of separating the solids obtained in the vacuum drying step, and passing the separated supernatant through an ion exchange resin filter to prepare a step of lyophilizing the extract.

In addition, the non-nuri can produce the outpost using the mountain or idle land, where the economic crops are not particularly seeded, so as not only to increase land use but also cost much to cultivate, economic effects can be obtained.

Non-Repair, Finitol, Blood Sugar, Diabetes

Description

A method of extracting a d-ounitol from Lespedeza cuneata and functional goods containing extracts}

1 is a flow chart showing a method for extracting finitol from non-repair according to the present invention.

The present invention relates to a method for extracting pinitol from non-repair and a functional food using the extract, in particular, to extract a blood glucose control substance finitol from non-repair (Lespedeza cuneata) to produce a blood glucose control functional food using natural finitol useful for diabetes It is about a method.

Beesuri (Lespedeza cuneata) means a herb that opens the bar at night, and when it is eaten, it is said to shine around outside the sky. It is also called the Great King (大力 王) because of the great power, and because it is chasing snakes. Non-auri has excellent effects in treating various male diseases, lack of nourishment, premature ejaculation, oil well, vulgaris, etc., especially in elderly people with weak kidney function. The leaves, roots, and stems of the rhinoceros contain flavonoids, finitol, phenol, tannin, and cytosterol, which remove inflammation and sputum, kill or inhibit staphylococcus aureus, pneumococci, streptococci, and catarrhals. (Kim Tae-Jung, Korea Plant Resources, Seoul National University Press, 1996; Jin-Kyu Choi, Our Herb, Flower, Tree, Korean Culture, 2001)

In general, for diabetics, glycemic control is often performed alone or in combination with medication, diet and exercise. In particular, Finitol, which has been known as an effective glycemic control ingredient for diabetic patients, is a natural blood sugar-enhancing ingredient contained in legumes and pine needles. In western India, a legume called Bougainvillea is traditionally used as a medicine for diabetic patients. Plant leaves have been used, and scientists have found that the active ingredient is pinitol.

In addition, there are reports that pinitol, an effective glycemic control agent, exists in the leaves of Dhaincha plants grown in India, monsoon climate, and Honeybush plants in Africa.

Finitol is the first known d-chiro-inositol in the stems of the sugar pine (Pinus lambertiana Dougl). That is, glucose extracted from pine is a kind of sugar alcohol having a methyl ester bond.

Finitol, a type of sugar alcohol, is known to be a useful ingredient for blood sugar control. The British pharmacological journal introduced the fact that pinitol acts like insulin ("Insulin-like effect of Pinitol", 2000, Vol. 130, 1944 ~ 1948p). Since then, many scholars have intensively studied the effects of glycemic fortification.

It is also known to be involved in helping blood sugar to be used as an energy source or to convert glucose into glycogen, which is known to be very effective in strengthening blood sugar. (Phytoglycemic effect of soybean isolated from soybean, Korea, Food Science and Industry, 36 : 1-5, 2003). It has been confirmed in animal studies that finitol has an effect of preventing cataract, liver and kidney damage, which are likely to be complications of diabetes.

One example of such a technique is Korean Patent Publication No. 2002-76095 (Invention name: Separation method of chiroinositol component from soybean processing by-product), Patent Publication No. 2004-35633 (Name of invention: Separation production method) and the like.

That is, the above-mentioned Patent Publication No. 2002-76095 discloses a method for separating chiroinositol by adsorption treatment on activated charcoal, fractionating and recovering a chiroinositol component, a finitol component, and an oligosaccharide component. In order to obtain d-chiro-inosito in soybean embryos, enzyme-reacted soybean embryo extracts were filtered, and insoluble material in the extracts was filtered with diatomaceous earth, and then chiro-inositol was prepared from soybean embryos by passing through an ionic resin. A method of doing so is disclosed.

In addition, Korean Patent Laid-Open Publication No. 2004-81256 (manufacturing method for reproductive health supplements containing pinitol ingredient) and Korean Patent Laid-Open Publication No. 2004-98839 (non-repair extract and its use), etc., extracts the pinitol and mixes it with cereals. A technique of adding water to non-repairing, hot water extraction, concentration and freezing and extracting is disclosed.

However, the technique described in the above publication discloses a method for separating chiroinositol, but since diatomaceous earth and the like must be regenerated or consumed each time, there is a problem in that the manufacturing cost increases and the manufacturing process is complicated.

In addition, in the technique described in the above-mentioned publication No. 2004-81256, there is a problem that the reproductive health supplements containing the pinitol component are merely described, and the process of extracting the pinitol component is not specifically described.

An object of the present invention was made to solve the conventional problems as described above, and extracts the pinotitol, a blood sugar control substance in non-repair, and using the extracted pinitol to extract a useful pinitol and blood glucose control function using the extract To provide food

In order to achieve the above object, a method for extracting finitol in non-repair according to the present invention comprises the steps of preparing an outpost in non-repair, adding the non-repair to ethanol and grinding with a homogenizer, crushed non-repair alcohol Precipitation step to stand, centrifugation step of centrifugation of the supernatant recovered in the precipitation step, decompression drying step of drying the supernatant recovered in the centrifugation step under reduced pressure, separation step of separating the solids obtained in the vacuum drying step, separated supernatant After passing through the ion exchange resin filter is characterized in that it comprises the step of lyophilizing the extract.

The precipitation step of the present invention is to be allowed to settle for about 1 hour in 4 ° C low temperature room, and is extracted according to the method of extracting finitol in non-repair. In addition, the active ingredient of the extract is characterized in that it controls the blood sugar of diabetic patients with finitol or d-chiro-inositol.

The above and other objects and novel features of the present invention will become more apparent from the description and the accompanying drawings.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.

Example 1

1 is a flow chart illustrating a method for extracting finitol from non-repair according to the present invention.

Preparation Steps-Step 1

Prepare 1 kg of fresh Non-Repair Outposts.

Addition and grinding step-step 2

1200 ml (v) of a 70% (v / v) ethanol solution was added to the prepared non-repair, and pulverized for 20 minutes using a homogenizer of 3500 rpm (revolution per minute) for lipolysis (S2). .

Sedimentation Stage-Stage 3

The pulverized solution mixed with the pulverized non-repair and ethanol is allowed to settle for about 1 hour in a low temperature room at 4 ° C. (S3)

Centrifuge-Step 4

After precipitation, the supernatant liquid, which is the supernatant liquid, is transferred to a new vessel, and 200 ml of ethanol 60% (v / v) solution maintained at 4 ° C. is added to the recovered supernatant, and then 50 ml of a flacon tube for centrifugation. After dispensing in and centrifuged for 20 minutes at 22000g (S4).

Decompression drying step-step 5

After recovering the supernatant again, transfer to a 3000ml reduced pressure flask and dried under reduced pressure (S5).

Separation Stage-Stage 6

50 ml of tertiary distilled water was added to the depressurized flask containing the solid obtained in step 5, and then dissolved. 20 ml of chloroform solution was added to the mixture and mixed well. (S6).

Freeze drying step-step 7

In step 6, the supernatant is transferred to a new flask and passed through an ion exchange resin filter for use as a sample for gas liquid chromatography, and the obtained extract is lyophilized (S7).
At this time, about 0.42 g of finitol is obtained from 100 g of dried non-repaired outpost.

The freeze-dried sample obtained above was dissolved in tertiary distilled water, and activated carbon was added at a rate of 3 mg (w / w) based on 1 mg of the sample, followed by filtration.

The gas-liquid chromatography described above was performed using OV-17 column of Oxime-TMS packing.

<Example 2>

In Example 2, non-repair was prepared as follows for a clinical trial for glycemic control of diabetic patients using the non-repair method and the functional food using the method according to the present invention.

Prepare 10.5 kg (W) of dried non-repaired plant ground (including leaves and stems).

42 liters (V) of distilled water was added to the prepared sample, and hot water was extracted for 12 hours under pressure conditions of 110 ° C. and 1.5 kg / cm 3.
At this time, about 0.42 g of finitol is obtained from 100 g of dried non-repaired outpost.

100 mL of the extracted sample was used as a sample of hot water extract for blood sugar control using a packing device.

Packing 100ml each sample extracted in the above process was confirmed that the content of finitol present in the non-repairing plant of Example 1 was 420mg (weight) per 100g (weight) of the sample and 100mg per 100ml (V) of the hot water extract sample. It was adjusted to be contained at a concentration of (W).

The basis for this calculation is that the dry repair ratio of 420 weight mg / dry ratio repair and the weight of dry weight of 50 weight g is required because the amount of phytinol required 200 days per day of adulthood.

For the clinical trial for glycemic control, the following subjects were examined for changes in blood glucose after eating the above extract containing 100 mg (W) of non-repaired pinitol, 30 minutes before breakfast and dinner every 30 days.

Blood Glucose Control Effects of Non-Repairing Extracts Target / Survey Items gender  Age (years) Weight (Kg) Diabetes history (years) Blood glucose before irradiation (mg / dl) Blood glucose after 30 days (mg / dl) Blood sugar reduction (%) Remarks A south 48 65     14 250 180 28.0 B female 37 53      7 220 135 38.6 C south 63 68     20 310 160 48.4 D south 52 66      4 235 184 21.7 E female 55 59      4 280 170 39.2 F south 67 63      9 205 155 24.4 G female 63 65     15 260 220 15.4 Average 55.0 62.7  10.4 251.4 172.0 30.8

In Table 1, the dried non-repaired 50g daily was extracted with hot water and administered 30 minutes before meals 30 minutes each morning, and fasting blood glucose levels before breakfast the next day.

As can be seen in Table 1, the present invention is about 15.4 ~ 48.4% (30 mg after pre-irradiation-blood glucose level after irradiation = changed blood glucose level / pre-irradiation glucose level) 30 days after the start of administration to diabetic patients from the extract containing the non-nurinate finitol X100), an average of 30.8% of blood glucose was improved.

Although the present invention has been described in detail according to the above embodiments made by the present inventors, the present invention is not limited to the above embodiments and can be variously modified without departing from the gist of the present invention.

In other words, in the above embodiment, the extraction of the extract, which is a method of extracting finitol from non-repair, has been described, but the present invention is not limited thereto, and of course, it can be added to other reproductive supplements and used.

As described above, according to the method for extracting finitol and the functional food using the extract according to the present invention, in the method for extracting the finitol, about 0.42 g of finitol was obtained from 100 g of dried non-repaired outpost, and about 60 kg of Considering that the amount of ingredient administered to adult diabetic patients with body weight is 200mg (W) or more, it is possible to provide a functional food for controlling blood sugar by extracting usefull for the diabetic patients from dried non-repaired starches of about 40 ~ 80g (W). The effect can be obtained.

In addition, according to the present invention, the non-nuri can produce outpost by using the mountain or idle land where economic crops are not particularly seeded. Therefore, not only the land use is increased, but also it does not cost much to cultivate.

Claims (5)

Preparing outposts in non-repair; An addition grinding step of adding the non-repair to ethanol and grinding with a homogenizer; A precipitation step of leaving the ground non-repair in alcohol; A centrifugation step of centrifuging the supernatant recovered in the precipitation step; A vacuum drying step of vacuum drying the supernatant recovered in the centrifugation step; A separation step of separating the solids obtained in the vacuum drying step; Passing the separated supernatant through an ion exchange resin filter, the method of extracting finitol from non-repair, characterized in that it comprises the step of lyophilizing the extract. The method of claim 1, The precipitation step is a method for extracting finitol from non-repair, characterized in that the precipitate is left standing for about 1 hour at 4 ℃ low temperature room. Non-repair extract, characterized in that extracted according to the method of extracting finitol in the non-repair according to claim 1 or 2. The method of claim 3, The non-repair extract is a non-repair extract, characterized in that the active ingredient is finitol. delete

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