KR100719761B1 - Cosmetic composition for atopic skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for atopic skin containing goat's milk and yangja extract. The cosmetic composition according to the present invention is excellent in safety for the skin and is substantially effective in preventing or alleviating atopic skin, and is easy to stabilize preservation when formulating cosmetics.

Atopic skin, goat's milk, nourishment, cosmetic composition

Description

A cosmetic composition for atopic skin

Figure 1 is a photograph of a three-dimensional artificial skin made of human cells cultured to determine the amount of IL-α released from goat milk and sheep extract mixture after inducing inflammation.

Figure 2 is a closed intelligence test results (24 hours later) to confirm the effect of erythema relief when applying the skin. Numerals 1 to 4 in the photographs show results when 1 is sodium lauryl sulfate, 2 is comparative prescription example, 3 is prescription example 1, and 4 is prescription example 1-1.

     Immunity is one of the most important functions in vivo. However, the function is not always beneficial to the living body. Allergic diseases, for example, excessive immune response, cause various damages to the living body.

Allergy is derived from the Greek word "allon argon", meaning "to try to react differently from normal," and as used in 1910 as a title written by a pediatrician in a book by Burke, a changed action of a person. ) Is meant. In the case of allergies, even though the foreign substance is not really harmful, it causes a lot of symptoms in our body as a result of an over-immune reaction. In other words, the external intruder is harmless, but it is mistaken as an overreaction. Allergies are basically classified as Types I to IV in the classification by Coombs and Gell. Among them, type I allergy is called "immediate type" allergy and causes atopic diseases. Type IV allergies are also called "delayed" allergies. Allergy type I is caused by an excessive immune response. Histamine-mediated reactions by IgE antibodies are typical, and asthma, allergic rhinitis, Atopic dermatitis and anaphylactic shocks are classified into atopic dermatitis. Of the four diseases, skin related is atopic dermatitis.

      Atopic skin is easily itch due to itching, eczema lesions occur, the skin moisturizing power is reduced to become rough skin. In addition, skin diseases caused by bacteria and viruses are easily generated.

  The term “Atopy” was first coined by Coca in 1923, and it is a (no) + top (place) + y (ness), meaning “weird” or “inappropriate” to food or inhalants. It refers to a constitution that has a genetic tendency to cause allergy easily. Literally, a variety of causes are entangled and complicated, recurring and recurring. Complex and diverse causes mean that treatment is difficult. Atopic dermatitis refers to a series of allergic symptoms that occur in the skin, respiratory mucosa, eye mucosa, and intestinal mucosa in individuals with atopic predisposition. These atopic predispositions (allergic predispositions) are inherited and appear as family history. Allergic diseases caused by atopy predisposition include allergic dermatitis, allergic rhinitis, asthma, allergic conjunctivitis, and atopic dermatitis. These diseases may be present singly or simultaneously.

Epidemiology Atopic dermatitis is a common skin disease that occurs in childhood. The frequency is 0.25%-20% and occurs in a ratio of male to female = 3: 2. In recent years, the incidence is increasing due to air pollution, increased exposure to antigens due to changes in residential environment, decreased breastfeeding, and reduced childhood infectious diseases. The onset of symptoms usually occurs between two and six months of age, especially under one year of age, with 85% occurring within five years of age. It is commonly known as a disease when young, but 50% of patients disappear within two stones, but 25% go to adolescence, and the remaining 25% do not go away even as adults. 80-90% of atopic dermatitis increases inflammatory cell activation and serum IgE, which is not necessarily proportional to the severity of dermatitis. When the antigen penetrates the human body, the antigen and IgE combine to excite mast cells and secrete inflammatory mediators such as histamine in the cell. These chemicals cause dermatitis and cause red spots, edema and pruritus. . Overproduction of IgE and cell-mediated immune abnormalities, which are immunological abnormalities in patients with atopic dermatitis, are known to interact with each other.

      At present, in order to alleviate or treat such atopic dermatitis, administration of steroid external preparations, antihistamines, anti-allergic agents, or herbal medicines, which are pharmacotherapy, is performed depending on symptoms. Of these, the use of external steroids is the most widely used method because it has an effect of alleviating inflammation and itching in a short period of time. However, long-term use of external steroids not only causes many side effects such as inhibiting cell proliferation, modulating cell function, and suppressing immune function, but also increases the amount of the drug used in order to produce a lasting effect. Indomethacin, which is used as an anti-inflammatory agent, is a substance that cannot be used in cosmetics, and licorice and its derivatives are difficult to stabilize or have poor solubility. Known anti-inflammatory drugs have problems in terms of skin safety and stability when formulating cosmetics, so their use is limited. In addition to external preparations, other preparations that are taken to prevent the release of itching, including histamine, which causes itching, may have the aforementioned side effects, and their therapeutic and palliative effects are not clear.

      In particular, the baby's skin, as well as the major appendages such as sweat glands, are still immature and easily lose moisture, become dry and resistant to damage. It is the same, but it is insufficient in terms of function, and since it is only about 3 years old, it becomes the thickness of adult skin, so skin protection is a basic requirement and important thing for babies. In addition, soft baby skin is thin and soft, sensitive to external stimuli and bacterial infections, and penetrating ability is much faster than adults, so sweat or bacteria remaining on the skin penetrates into the skin again, which causes the skin to crush and other skin. Trouble may occur. In other words, baby's skin is not only soft, delicate, but also sensitive, so it is easy to cause skin troubles even with small external stimuli. Therefore, it is also important to select and use raw materials without irritation while enhancing skin immunity. <See BAYER KOREA, Bayer HEALTHCARE>

     As such, baby skin tends to become dry skin, and this dry skin can weaken immune function and develop into various skin diseases as well as atopic dermatitis, so weaker and more sensitive skin need preventive measures.

      Accordingly, the present inventors completed the present invention by preparing a cosmetic composition comprising the goat oil and yang extract mixture as an active ingredient, which is excellent in safety on the skin and is substantially effective in preventing or alleviating atopic skin, and is easy to stabilize and preserve during cosmetic formulation. It was.

      As an example of the prior invention, Korean Patent Application No. 10-2004-7013313 discloses a mammalian oil by granules in which at least five to six kinds of functions such as lactic acid and yeast are incorporated into a seed called kefir grains. Discloses a skin external preparation composition comprising a fermented milk product obtained by fermenting soy milk and skim milk powder and the like or a pharmaceutically or cosmetically acceptable carrier. Korean Patent No. 10-0030790 discloses the main ingredient of animal milk. Whey was collected from the culture obtained by inoculating lactic acid bacteria in a culture incubator, and the whey was heated to about 30-50 ° C. under reduced pressure, and the whey was evaporated by about 10-20% to acetaldehyde and acetone in whey. And a method for producing a moisturizer for skin, characterized in that the evaporation of a portion until the diacetyl component is less than 0.5ppm is disclosed. These compositions differ from the composition of this invention which has the effect of preventing or alleviating atopic skin.

      On the other hand, the Republic of Korea Patent No. 10-0552617 discloses the purified Lavda-12-ene-15,16-dial compound and its separation and purification method under the loading, but this component has an antimicrobial activity and anticancer activity It is irrelevant to the cosmetic composition described and having a prophylactic or alleviating effect of atopic dermatitis to be achieved in the present invention.

      In addition, European Patent Application EP 1 319 407 discloses a pharmaceutical composition for topical treatment of skin disorders and skin wounds, including milk globules, which centrifuges sheep's milk rather than goat's milk. The milk fat globule obtained by using as an active ingredient and used for the treatment of acne, burns, excess sebum, including goat's milk extract in addition to goat's milk composition is different from the composition of the present invention that has the effect of preventing or relieving atopic skin . In addition, US patent application US 10/996410 discloses a cosmetic composition comprising lamb's milk, water, at least one vegetable oil and an emulsifier, and a method for preparing the same, which is used for the prevention of atopic skin including the goat's milk and the medicinal extract mixture of the present invention. Or the structure differs from a composition for relaxation.

Therefore, the technical problem to be achieved by the present invention is to solve the above problems and to suppress the type 1 allergy causing itching and inflammation caused by atopic dermatitis, as well as for skin redness, dry skin, erythema and the like. It is to provide a cosmetic composition for the prevention or alleviation of atopic dermatitis that can be used safely without irritation.

      The present invention relates to a cosmetic composition for atopic skin containing goat's milk and yangja extract.

     Goats, also called goats, are useful species that use milk, broilers that use meat, and combinations. Black goats are broilers, and lactose using milk is a useful species. Goat milk is milk squeezed from goats, the composition of which is 88.0% moisture, 3.1% protein, 3.6% fat, 4.5% lactose and 0.8% ash. Calories are 62 kcal per 100 g, which is higher in protein, fat, and ash than milk and has a higher percentage of albumin. Goat milk fat particles (脂肪 粒 子) is small to form a light curd (curd) is high digestibility, and because the carotene is less than milk, the color looks white. Casein Micelle, a protein source of milk, is composed of three components: alpha S-1 casein, beta casein and gamma casein. Goat milk, unlike regular milk, is a major component of beta casein. When goat milk or milk enters the human body, enzymes penetrate and decompose into the linkage of essential amino acids, including protein, to facilitate digestion. In this case, casein micelle of large milk is difficult to break down. On the other hand, casein micelles of goat's milk are small in size and are easily digested due to enzyme penetration. Also, unlike milk, goat's milk contains little alpha S-1 casein, so it is digested smoothly. Unlike milk formula, goat milk made with goat's milk has similar ingredients to breast milk, especially as a substitute for babies with allergies or low digestibility. Iron and zinc in goat's milk are well absorbed and have high bioavailability to aid growth and development, and contain 27% more antioxidant selenium than milk. Since breast milk is a complete food for babies, the nutritional value of proteins can be compared with the composition of breast milk proteins. In that sense, goat's milk is the food closest to human milk, which is the most suitable food for human body. In Korea and Japan, most households drink alcohol, but on the Mediterranean coast, goat's milk is a representative food that has a strong positive energy and is known as a good food for sourness. Weak foods have been known for the elderly and the elderly. Hence, goat milk has been called “milk of the poor” since ancient times, and Indian Gandhi always drank goat milk to recover the body after a long fast.

Zingiber mioga ) is a ginger plant native to tropical Asia. Roots stalked sideways and covered with scaly leaves. The leaves are lanceolate or long oval shape, and the bottom part is leaf shape, and they grow in the shape of stems while wrapping each other up to 40-100cm in height. Flower is yellow in August-October, 5cm in diameter, and runs in long oval inflorescence on stalk. The stalk is wrapped in scaly leaves at the end of the rhizome and reaches 5-7 cm in length. Po is narrow egg-shaped and pointed at the end. Calyx is tubular, corolla is divided into three. Lip petal is the largest of the three split and split pieces, with one stamen. The stalk is edible before flowering and the stem is edible before spring. In oriental medicine, root stems and seeds are used as medicinal herbs. Root stems are used to treat women's menstrual disorders and white crabs, and have antitussive and expectorant effects. Seeds are taken by adding sugar and water when the stomachache is severe.

      When the composition is used in the cosmetic composition, in addition to the active ingredient of the goat's milk and the medicinal extract as described above, it contains components commonly used in cosmetics. For example, conventional adjuvants such as thickeners, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers and the like.

      Cosmetics of the present invention can be prepared in any formulation commonly prepared in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, It is not limited to this. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

      The cosmetic composition of the present invention is derived from food itself and has little toxicity and side effects, and thus can be safely used as a cosmetic material.

      The cosmetic composition according to the present invention can be prepared by mixing the dry extract obtained by extracting medicament with sterile goat milk.

      The preparation method of the medicinal extract is as follows. First, 5-20 times one or more solvents selected from the group consisting of water, anhydrous or hydrous ethanol, butanol, acetone, ethyl acetate, hexane, butylene glycol, and propylene glycol are extracted as dry solvents by weight. . It is equipped with a cooling condenser to prevent the solvent from evaporating and extracted by heating at 50-95 ℃ for 3-20 hours, or by extracting the active ingredient by deposition at 5-37 ℃ 1-15 days. have. The extracted extract is concentrated under reduced pressure while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser, and then lyophilized or spray dried to obtain a dry extract. The extract may be prepared by appropriate selection in a wide range of 0.01 to 10.0 (w / v)% in terms of solids relative to the total extract, and preferably, may be included in an amount of 0.1 to 5.0 (w / v)%. .

      Goat milk is cooled after sterilizing the goat milk obtained from the goat ranch using the pasteurization method of conventional milk. Sterilized goat's milk is removed using activated charcoal to remove odors and salts that can cause skin irritation. Thereafter, using a microfluidizer or the like can be homogenized to be more easily absorbed by the skin, and mixed with a certain amount of extract and a certain ratio can be prepared a mixed extract.

The mixture of goat's milk and sheep's extract may be appropriately selected and included in a wide range of 1-10% based on the total weight of the composition when formulating cosmetics.

The cosmetic composition according to the present invention not only has an excellent effect of suppressing type 1 allergy causing itching or inflammation caused by atopic dermatitis, but also can be used safely without irritation to skin such as skin redness, dry skin, erythema and the like. It is easy to stabilize preservation when compounding.

The following Examples and Experimental Examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

<Example>

Example  1-1. Manufacturing method of spearmint extract

      500 g of fully dried sheep was put in 10 kg of distilled water, and extracted by boiling for 5 hours in an extractor equipped with a cooling condenser. Thereafter, the extract was filtered through a 400 mesh filter cloth, cooled to room temperature, left to mature at 5-15 ° C. for 7 days, and then filtered using Whatman No. 2 filter paper. The extract was filtered with a 0.45 μm filter, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser to obtain 20.2 g (dry weight).

Example  1-2 ~ 1-7

Using the solvent of Table 1 below and extracting in the same manner as in Example 1-1 and the results are shown in Table 1 below.

Fermentation Extract and Yield According to Solvent Composition Extraction solvent Solid content (%) Yield (g) Example 1-1 Distilled water 1.98 480 Example 1-2 Ethyl alcohol 2.0 473 Example 1-3 30% hydrous ethyl alcohol 2.4 498 Example 1-4 Butylene glycol 2.6 495 Example 1-5 30% water butylene glycol 2.5 479 Example 1-6  Propylene glycol 2.12 472 Example 1-7 30% water propylene glycol 2.1 474

Example  2 Goat milk  Manufacturing method

      50L of goat's milk obtained from Daegwallyeong, Gangwon-do was sterilized at 65 ° C for 30 minutes, cooled to 4 ° C, and 50g of activated carbon of 80-100 mesh (Aldrich) was stirred for 1 hour. Goat milk mixed well with activated carbon was centrifuged at 10,000 rpm, and then filtered sequentially using filter paper having a particle size of 0.8 μm, 0.4 μm, 0.2 μm, and 0.1 μm. The filtered goat milk was homogenized three times using a microfluidizer at a pressure of 1,000 bar to obtain 45.2 L of homogenized goat milk.

Spare Experimental Example  One.

      In order to investigate the prevention and alleviation effect of atopic dermatitis of the extracts extracted with various solvents, the stimulation was induced by culturing the dermis and then the amount of IL-1α released by each extract was examined. Among them, the extract of the extract solvent showing the best activity was used in combination with goat's milk.

      IL-1α (interleukin-1α) is an intercellular cytokine that acts as a pre-inflammatory mediator and acts as a potential derivative of wound re-epithelialization. It is known to enhance the production of arachidonic acid lipoxygenase metabolites such as leukotriene B4, 5-, 12- and 15 HETE, which promotes leukocytosis as an allergic and inflammatory response regulator.

A 7: 1: 1 buffer solution prepared with Type I collagen matrix (Bioland, Korea), 5-fold concentrated DMEM (Dubelcco's Modified Eagle Medium, GIBCO BRL, USA), and 2.2% NaHCO ₃ , 200 mM HEPES and 0.05 N NaOH The mixed human fibroblasts cell line (ATCC, American Type Culture Collection, Cat.No. CRL-2076) was mixed at a density of 1 × 10 ⁵ cells / ml, and 200 μl of fibroblasts each were inserted into a 10 mm diameter insert. Prepare the dermis by adding the mixed collagen solution. After dermal preparation, the DMEM containing 10% FBS is replaced every 2 days and incubated for 7 days. 10 μl of skin irritant (SLS: sodium lauryl sulfate) is administered on the cultured dermis, and 10 μl of each extract is applied after 4 hours, followed by incubation at 37 ° C. and 5% CO ₂ for 24 hours. IL-1α was sampled using a human IL-1α assay kit (Endogen Inc. Boston, MA, USA) and measured at 450 nm by ELISA-reader (enzyme-linked immunosorbent assay). The results are shown in Table 2.

Anti-inflammatory effect in the dermis Preliminary Example Extraction solvent Final concentration of the extract contained in the reaction solution (㎍ / ml) IL-1α release amount (pg / ml) SLS (Inflammatory Substance)  0.07% 89.7 Preliminary Example 1 Distilled water 100 66.5 Preliminary Example 2 Ethyl alcohol 100 73.9 Preliminary Example 3 30% hydrous ethyl alcohol 100 69.3 Preliminary Example 4 Propylene glycol 100 69.6 Preliminary Example 5 30% water propylene glycol 100 63.9 Preliminary Example 6 Butylene glycol 100 67.3 Preliminary Example 7 30% water butylene glycol 100 59.8

Goat Milk  Preparation of Unloading Extract Mixture

     Goat milk and Yangha extract was mixed in the mixing ratio as shown in Table 3, and then the effect of preventing or mitigating atopic dermatitis was measured.

Goat Milk and Ferment Extract Blend Ratio  Example Mixing ratio of extract (%) Goat milk Spear extract  Example 1 0 100  Example 2 10 90  Example 3 20 80  Example 4 30 70  Example 5 40 60  Example 6 50 50  Example 7 60 40  Example 8 70 30  Example 9 80 20  Example 10 90 10  Example 11 100 0

Experimental Example 1 . mouse On ear edema  Anti-inflammatory experiment

     In order to examine the anti-inflammatory effects on goat's milk and sheep's extract mixture, the mouse's left ear was used as the control site and the right ear as the test site. After continuous application, 1 hour after the last application, ethanol was applied to the left ear, and 2 mg / ear of arachidonic acid was applied to the right ear. After 1 hour, the ear edema was repeated three times with both micrometers. Measured.

     Anti-inflammatory effect was determined as the degree of edema inhibition based on Arachidonic acid treatment group and the results are shown in Table 5.

※ Inhibition Rate (%) = (A―B) / A × 100

         A: Average thickness of control ears (thickness of arachidonic acid treated ears-thickness of untreated ears)

         B: thickness of the sample-coating group ear (thickness of the sample-treated ear-thickness of the untreated ear)

Inhibition of Ear Thickness and Inflammation by Topical Application of Goat Milk and Fermented Extracts sample Sample throughput menstruum Ear thickness (㎛) % Inhibition Before sample processing After sample processing Indomethacin 1.0 µg / ml ethanol 284 421 45.6 Arachidonic acid 2mg / ear ethanol 299 551 - Example 1 100 μl ethanol 284 505 12.4 Example 2 100 μl ethanol 294 511 13.8 Example 3 100 μl ethanol 295 503 17.4 Example 4 100 μl ethanol 295 498 19.6 Example 5 100 μl ethanol 263 466 19.5 Example 6 100 μl ethanol 279 495 14.3 Example 7 100 μl ethanol 288 499 16.4 Example 8 100 μl ethanol 284 487 19.3 Example 9 100 μl ethanol 283 472 25.0 Example 10 100 μl ethanol 296 497 20.2 Example 11 100 μl ethanol 279 477 21.4

Experimental Example  2. Measurement of Growth Activity of Immune Cells

MTT assay is a method widely used for cell proliferation and toxicity by measuring the number of living cells. The living cells are water-soluble and yellow salts of MTT (3- [4,5-dimethylthiazole-2-yl] -2, in the mitochondria. 5-diphenyltetrazolium bromide) is used to reduce the water-insoluble blue formazan derivative by succinate dehydrogenase. The resulting formazan derivative is dissolved in a dissolving agent and then measured for absorbance. In more detail, the human immune cells Raji (Human B cell) and Jurkat (Human CD4 ⁺ T cell) were analyzed by MTT (3- [4,5-dimethylthiazole-2-yl] -2,5-diphenyltetrazolium bromide) assay. It was carried out using. Cell growth was incubated in 37%, 5% CO ₂ incubator using 10% FBS in RPMI1640 (GIBCO, USA) medium. For the measurement of immune cell growth activity, each sample is applied to each concentration after 24 hours of incubation with a cell concentration of 4x10 ⁴ cells / ml in a 24 well plate. After 48 hours of incubation, the medium in each well was discarded, 1000 μl of MTT solution was added to each well, and after 4 hours, MTT was removed, and 1000 μl of DMSO was added to the well, and then absorbed at 570 nm after incubation at 37 ° C. overnight. . At this time, the experiment was conducted in the same manner without adding the goat's milk and sheep extract mixture as a control to measure the difference in relative absorbance over a total of 8 days to observe the growth activity of the cells. The results are shown in Table 5.

As a result of measuring the growth activity of immune cells using Raji (Human B cell) and Jurkat (Human CD4 ⁺ T cell) which are human immune cells, it can be seen that when Example 9 was applied, it showed the best growth activity to increase immune activity. have.

Evaluation of Immune Cell Growth Activity of Goat Milk and Fermented Extracts      Example Final concentration of goat's milk and dipping extract mixture in the reaction solution (㎍ / ml) Growth activity (%) B cell T cell   Example 1 200 100 102  Example 2 200 103 101  Example 3 200 101 106  Example 4 200 104 107  Example 5 200 105 109  Example 6 200 107 106  Example 7 200 105 111  Example 8 200 112 109  Example 9 200 117 120  Example 10 200 113 110  Example 11 200 110 107

Experimental Example  3. Anti-allergy  Experiment (β- hexosaminidase  Emission measurement)

     β-hexosaminidase is Enzymes contained in mast cells are released with histamine when the mast cells are activated by an immune response. Therefore, it can be used as an indicator of degranulation of mast cells. <Planta Med. 64, 577-578 (1998)>

RBL-2H3 cells are incubated with EMEM containing 10% FBS and glutamine. Dispense ⁵ x 10 ⁵ cells per well into a ²⁴ well plate. At this time, using medium containing 0.5μg / ml mouse monoclonal IgE in EMEM, incubated in 37 ℃, 5% CO ₂ incubator for 24 hours. Rinse the cells with 500 μl of siraganian buffer I per well, add 160 μl of siraganian buffer II, and incubate in 37 ° C., 5% CO ₂ incubator for 10 minutes. After treating the goat and sheep mixed extract and incubated for 20 minutes in 37 ℃, 5% CO ₂ incubator and treated with antigen (1㎍ / ㎖, DNP-BSA) for 10 minutes. The reaction is stopped in a ice bath for 10 minutes to stop the reaction and centrifuge. 20 μl of substrate (1mM ρ-nitrophenyl-N-acetyl-β-D-glucosaminide) was added to the supernatant, and then incubated for 1 hour at 37 ° C and 5% CO ₂ incubator, followed by reaction with 0.1M Na ₂ CO ₃ / NaHCO ₃ . To stop. Absorbance was measured at 405 nm using an ELISA reader.

※ Inhibition Rate (%) = (B-C-D) / (A-C-D)

-A (control): no test material added (allergen IgE reaction measurement)

-B (treated): Test material added (allergen IgE reaction measurement)

-C (blank): add only test material and substrate to ELISA plate

-D (spontaneous): Antigen, IgE, test material not added

Antiallergic Evaluation of Goat Milk and Fermented Extracts sample Final concentration of goat's milk and dipping extract mixture in the reaction solution (㎍ / ml)  β- hexosaminidase % Inhibition of release Example 1. 200 21.3 Example 2. 200 23.6 Example 3. 200 27.9 Example 4. 200 32.8 Example 5. 200 25.6 Example 6. 200 29.9 Example 7. 200 30.4 Example 8. 200 35.8 Example 9. 200 45.8 Example 10. 200 39.4 Example 11. 200 35.9

Experimental Example  4. allergy  evaluation( LLNA assay )

To confirm allergy to the goat and ram extract mixture, apply 25µl sample to the mouse ear once a day for 3 days, remove lymph nodes on day 4, separate lymphocytes, and incubate for 24-48 hours in a 5% carbon dioxide incubator. The degree of amplification is measured by the amount of radioisotope [ ³ H] -methyl thymidine inserted. The local lymph node assay (LLNA) results indicate the lymphocyte amplification of the sample group compared to the control group, and allergens when the stimulation index (SI) of the sample is more than 3 and increases by concentration in one concentration. Considered as.

※ (counter per minute) -cpm: [3 H] - methyl-thymidine (methyl thymidine) amount to be inserted into the cell

       S.I (stimulation index): The mean cpm of the sample divided by the mean cpm of the control.

      As shown in Table 7, the stimulation index (S.I) of the extract was found to be almost allergic to less than 3.

Allergy Evaluation Results of Goat Milk and Fermented Extracts sample Final concentration of goat's milk and dipping extract mixture in the reaction solution (㎍ / ml) Average cpm Amplification degree (S.I) Ethanol (60%) 100 2261 ± 121 ― Example 1 100 3215 ± 125 1.86 Example 2 100 3233 ± 265 1.62 Example 3 100 3187 ± 288 1.54 Example 4 100 2993 ± 231 1.98 Example 5 100 4533 ± 215 1.45 Example 6 100 4238 ± 241 1.27 Example 7 100 4548 ± 331 2.01 Example 8 100 4522 ± 135 1.70 Example 9 100 4002 ± 195 1.74 Example 10 100 4566 ± 301 1.59 Example 11 100 4577 ± 302 1.32

Prescription Example  One.

      The prescription example of the nutrition lotion in the cosmetics containing the goat's milk and nourishing extract mixture of Example 9 is as follows.

number Raw material Prescription Example 1 Prescription Example 1-1 Comparative Prescription 1 One  Goat milk and sheep extract mixture (Example 5) 5.0 10.0 - 2  Beeswax 1.0 1.0 1.0 3  Polysorbate 60 1.5 1.5 1.5 4  Solbitan Sesquioleate 0.5 0.5 0.5 5  Floating paraffin 10.0 10.0 10.0 6  Sorbitan stearate 1.0 1.0 1.0 7  Lipophilic Monostearic Acid Glycerin 0.5 0.5 0.5 8  Stearic acid 1.5 1.5 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 1.0 1.0 10 Propylene glycol 3.0 3.0 3.0 11 Carboxy Polymer 0.1 0.1 0.1 12 Triethanolamine 0.2 0.2 0.2 13 antiseptic a very small amount a very small amount a very small amount 14 Pigment a very small amount a very small amount a very small amount 15 Spices a very small amount a very small amount a very small amount 16  Purified water Remaining amount Remaining amount Remaining amount 17 water - - 10

      <Production method>

Formulation Examples 1, 1-1: 10, 11, 13, 16 while stirring and mixing to 80 to 85 ℃ to put into the manufacturing unit and then actuated emulsifier 2, 3, 4, 5, 6, 7, 8, 9 and 12 were heated to 80 to 85 ° C., and then emulsified. After emulsification, heat-cool to 50 ℃ while stirring using a stirrer, add 15 times, cool to 45 ℃, add 14 times, add 1 to 35 ℃, cool to 25 ℃, and mature. .

Comparative Example : 10, 11, 13, 16 while stirring and mixing to 80 to 85 ℃ heated to the manufacturing unit after the operation of the emulsifier and 2, 3, 4, 5, 6, 7, 8, 9, 12 80 Emulsify after heating to ~ 85 ℃. After emulsification, heat-cool to 50 ℃ while stirring using a stirrer, add 15 times, cool to 45 ℃, add 14 times, add 17 times to 35 ℃, cool to 25 ℃, and mature. .

Prescription Example  2

      The prescription example of the nutrition cream in the cosmetics containing the goat's milk and the nourishing extract mixture of Example 9 is as follows.

number Raw material Content (% by weight) One Goat milk and sheep extract mixture (Example 5) 5.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / Capric Triglycerides 4.0 12 Carboxy Vinyl Polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Remaining amount

      <Production method>

      12, 13, 14, and 16 were mixed and stirred at 80 to 85 ° C. and added to the manufacturing unit, followed by actuating an emulsifier. It is heated to 85 ℃ and added, and 15 is emulsified. After emulsification, the mixture is cooled to 35 ° C. while stirring using a stirrer, 1 time is added, cooled to 25 ° C., and aged.

Prescription Example  3.

      Prescription example of the wash foam in the cosmetic composition containing the goat oil and yang extract mixture of Example 9 is as follows.

number        Raw material Content (% by weight) One  Goat Milk and Fertilizer Extract Mixture (Example 5) 5.0 2  T.A.-Cocoyl Glutamate 30.0 3  Disodium laureth sulfosuccinate glycerin 10.0 4  glycerin 10.0 5  Cocamide D.A. 2.0 6  P.G.-120 Methylglucose Dioleate 1.0 7  Methylgluses-20 0.5 8  P.E.G.-150 Pentaerythritol Tetrastearate 0.5 9  Tetrasodium E.D.T.A. 0.05 10  antiseptic a very small amount 11  Spices a very small amount 12  Purified water Remaining amount

      <Production method>

     2,3,4,5,6,7,8,9,10 are sequentially added to the manufacturing unit and heated to 60 to 65 ℃ and stirred for 15 minutes. After stirring, part of 12 was added, and after stirring for 30 minutes, part of 12 was slowly added. After stirring for 30 minutes, the mixture was cooled to 35 ° C., 1,11 was added, cooled to 25 ° C., and aged.

Prescription Example  4.

     A prescription example of the shampoo in the cosmetic composition containing the goat oil and yang extract mixture of Example 9 is as follows.

number        Raw material Content (% by weight) One  Goat Milk and Fertilizer Extract Mixture (Example 5) 5.0 2  T.A.-Cocoyl Glutamate 35.0 3  Sodium laureth sulfate 10.0 4  glycerin 5.0 5  Cocamide D.A.A 3.0 6  Disodium laureth sulfosulcinate 2.0 7  Laures-2 1.5 8  P.E.G.-150 Pentaerythritol Tetrastearate 1.5 9  Linolaminopropyl P.-dimonium chloride phosphoric acid 1.5 10  Sodium cocoyl isethionate 1.0 11  Olive Oil P.G.-7 Ester 1.0 12  P.G.-120 Methylglucose Dioleate 1.0 13  Methylgluses-20 1.0 14  P.E.-12 Dimethicone 0.7 15  Polyquaternium-7 0.5 16  Polyquaternium-10 0.1 17  Tetrasodium E.D.T.A. 0.1 18  antiseptic Quantity 19  Spices Quantity 20  Purified water Remaining amount

      <Production method>

     2,3,4,5,6,7,8,9,10,11,12,13,14,17,18 are sequentially added to the manufacturing unit, heated to 60 to 65 ℃ and stirred for 30 minutes. Part of 15, 16, 20 is added separately in the melting part, stirred for 30 minutes, then added to the manufacturing part and stirred for 30 minutes. Again, a portion of 20 was slowly added and stirred for 30 minutes, then cooled to 35 ° C, 1,19 was added, cooled to 25 ° C, and aged.

Application example  1. 3D In artificial skin  Anti-inflammatory IL -1α emission measurement)

     IL-1α (interleukin-1α) is an intercellular cytokine that acts as a pre-inflammatory mediator and acts as a potential derivative of wound re-epithelialization. It is known to enhance the production of arachidonic acid lipoxygenase metabolites such as leukotriene B4, 5-, 12- and 15 HETE, which promotes leukocytosis as an allergic and inflammatory response regulator. After the three-dimensional artificial skin was cultured to induce inflammation, the amount of IL-1α released from the goat and ram extract mixture was investigated.

      The lotion of prescription example 1,1-1 and the comparative prescription example 1 were used for the test.

A 7: 1: 1 buffer solution prepared with Type I collagen matrix (Bioland, Korea), 5-fold concentrated DMEM (Dubelcco's Modified Eagle Medium, GIBCO BRL, USA), and 2.2% NaHCO ₃ , 200 mM HEPES and 0.05 N NaOH The human fibroblasts cell line (ATCC, American Type Culture Collection, Cat.No. CRL-2076) was mixed at a density of 1 × 10 ⁵ cells / ml, and a collagen solution containing 200ul of fibroblasts in each 10mm diameter insert was prepared. To prepare the dermis. After dermal preparation, the DMEM containing 10% FBS is replaced every 2 days and incubated for 7 days. Inoculate 3 × 10 ⁵ cells / insert of normal keratinocytes onto cultured dermis. 7-day submerge culture with DMEM (10% FBS) and K-SFM (Keratinocyte Serum Free Medium, GIBCO, USA) (supplemented with EGF (epidermal growth factor) and BPE (bovine pituitary extract) In order to cultivate at the gas-liquid interface (air-liquid interface culture), the medium in the insert was removed and the medium used for the submerge culture outside the insert was cultured for 14 days using the air-liquid interface culture. After removal, 1.5 ml of fresh medium is added to the tissue culture vessel, and 10 µl of skin stimulant (SLS: sodium lauryl sulfate) is injected into the insert. Incubate for 24 hours at 5% CO ₂ The concentration of SLS, goat milk, and nutrient extract mixtures was the percent by weight of the toxic substance in solution IL-1α was obtained by taking a sample of the medium and using the Human IL-1α assay kit (Endog). en Inc. Boston, MA, USA) and measured at 450 nm by ELISA-reader (enzyme-linked immunosorbent assay) The results are shown in Table 8.

Anti-inflammatory Effects on 3-D Bioartificial Skin sample Final concentration of goat's milk and dipping extract mixture in the reaction solution (㎍ / ml) IL -1α emission amount ( pg Of ml ) SLS  0.07% 79.3 Comparative Prescription 1 50 73.2 Prescription Example 1 50 62 Prescription Example 1-1 50 50.1

Application example  2. Primary skin  Efficacy of erythema after stimulation ( Closed Patch Experiment )

      In order to check the effect of erythema on skin application, add 0.5% Sodium Laurly Sulfate, a skin stimulant, to the nutrition lotion of Prescription Example 1 and Comparative Example 1-1. The experiment was conducted. 20 healthy males, females and children aged 3 or older (age distribution: 3-8 years, average age: 5 years old, 15 men, 5 women).

The erythema index (E-index) is measured before applying the sample to the inside of the forearm of both sides of the subject, and a certain amount (0.2 g) of each sample is measured for 24 hours using a large finn chamber (Epitest, Hyryla Finland) and filter paper. The patch was attached during. The patch was removed and the erythema index was measured after the subject exposed the test site in a space without air movement and in direct sunlight, and then stabilized for 30 minutes. Dermaspectometer (Cortex Technology, Hadsund, Denmark) after the measurement using the results are shown as follows. The room temperature was 22 ° C. and the humidity was 43%.

Skin Primary Erythema Relief Test Results sample BL DAY0 DAY1 DAY2 WEEK1 Sodium Lauryl Sulfate  0.5% 8.9 16.9 14.1 13.3 11.1    compare Prescription Example  One 8.9 14.8 12.1 10.3 10.1 Prescription Example  One 9.0 12.9 10.7 9.8 9.5 Prescription Example  1-1 8.8 12.3 10.2 9.4 9.2

      As a result of the investigation, it can be seen that the skin erythema relief effect is superior to that of Formulation Example 1 and Comparative Example 1-1 prepared without the addition of goat's milk and yang extract mixture.

Application example  3. For skin sensitization  Inhibitory effect of contact hypersensitivity

      The lotion of prescription example 1,1-1 and the comparative prescription example 1 were used for the test.

     C57BL / 6 mice, 8-10 weeks old, who had undergone a one-week acclimation period, had their abdomen removed and irradiated with UV B 2.4KJ / ㎡ using an FS-20 sun lamp. It was observed that the inhibition compared to the. The lotions of Formulations 2 and 2-1 and Comparative Formula 2 were applied to the mouse abdomen after UV irradiation, and after 3 days, sensitized with 3% trinitrochlorobenzene in the UV irradiation area. After 6 days, 1% trinitrochlorobenzene was applied to the ears of mice and the edema status was measured.

sample  Immune enhancing effect (%)    compare Prescription Example  One 2.7 Prescription Example  One 18.9 Prescription Example  1-1 25.6

      The lotion of Formulation Example 1,1-1 and Comparative Example 1 were prepared and tested as a result of contact hypersensitivity by skin sensitization. When Formulation Examples 1 and 1-1 were applied, the secondary skin after the first stimulation The effects of sensitization on skin irritation, erythema and edema are effectively reduced, suggesting that the immune enhancing effect is improved.

Claims (11)

      Cosmetic composition for atopic skin containing goat's milk and yang extract. The cosmetic composition according to claim 1, wherein the extract is extracted by dipping 1-15 days in a solvent at 10-37 ° C. The cosmetic composition according to claim 1, wherein the extract is extracted by adding a solvent at 50-100 ° C. for 3-20 hours. A solvent according to claim 2 or 3, wherein the solvent used for extraction is water, anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol, and propylene glycol Cosmetic composition, characterized in that one or more solvent selected from the group consisting of. The cosmetic composition according to claim 1, which is a lotion (skin lotion), nutrition lotion, nutrition cream, massage cream or nutrition essence.       The cosmetic composition according to claim 1, which is a baby lotion (skin lotion), lotion, cream, sunscreen lotion or sunscreen cream. The cosmetic composition according to claim 1, which is a baby bath cleaner, shampoo or soap.      The composition of claim 1, wherein the extract is contained in an amount of 0.01 to 10.0% (w / v) in solids content of the total extract. 9. The composition of claim 8, wherein the extract is contained in an amount of 0.1 to 5.0% (w / v) in solids content of the total extract.       The composition of claim 1, wherein the goat's milk and the medicinal extract comprise 1 to 10% by weight of the total weight of the composition.        A process for preparing the composition of claim 1 comprising the steps of 1) to 5):         1) preparing dried loading;         2) extracting by heating or depositing by adding a solvent to the dried amount;         3) concentrating and drying the dipping extract of 2) to obtain a dry extract;         4) sterilizing the goat's milk;         5) removing impurities of the goat milk of 4);         6) homogenizing the goat's milk of 5); And  7) mixing the sheep extract of 3) and goat milk of 6)

Images