KR100521505B1 - UV-pretective constituents of gisenosides - Google Patents

Abstract

The present invention relates to a cosmetic composition for protecting against UV rays containing ginsenoside Rh ₂ of the following structural formula (1) and ginsenoside Rg ₃ of the following structural formula (2). More specifically, the present invention relates to a cosmetic composition for sunscreen comprising ginsenoside Rh ₂ and ginsenoside Rg ₃ in a ratio of 1: 1 to 1: 5. The composition containing ginsenoside Rh ₂ and ginsenoside Rg ₃ of the present invention in a ratio of 1: 1 to 1: 5 is excellent in UV blocking effect and has no side effects on the skin. It can be manufactured in cosmetics and applied to the skin.

Description

A cosmetic composition for protecting against ultraviolet rays, characterized by containing ginsenoside Rh2 and ginsenoside Rg3 {UV-pretective constituents of gisenosides}

The present invention relates to a cosmetic composition for protecting against UV rays containing ginsenoside Rh ₂ of the following structural formula (1) and ginsenoside Rg ₃ of the following structural formula (2). More specifically, the present invention relates to a sunscreen cosmetic composition comprising ginsenoside Rh ₂ and ginsenoside Rg ₃ in a ratio of 1: 1 to 1: 5. The composition containing ginsenoside Rh ₂ and ginsenoside Rg ₃ of the present invention in a ratio of 1: 1 to 1: 5 is excellent in UV blocking effect and has no side effects on the skin. It can be manufactured in cosmetics and applied to the skin.

Structural Formula 1.

Structural Formula 2.

Skin is an important organ that protects the body physically from the outside of the human body. Especially, the beauty of the skin is important in social life, and cosmetics for protecting and enhancing the skin have been widely used from whales.

The skin is damaged by various factors, especially in the spring and summer when the sun's light is intense. When the skin is damaged by ultraviolet rays, it can even cause skin cancer in severe cases, and even if it does not lead to skin cancer or other diseases, it causes various kinds of abnormalities to the skin, damaging the skin and harming the next.

Therefore, cosmetics for protecting the skin from ultraviolet rays have been widely developed in the past, and among them, there was also the development of cosmetics using extracts such as ginseng.

However, cosmetics or external skin preparations using such ginseng extracts have a disadvantage that the effect is insufficient to protect the skin from ultraviolet rays.

Therefore, the present inventors have long researched to develop an excellent skin external preparation that can sufficiently protect the skin from ultraviolet rays, and as a result, ginsenoside Rh ₂ and ginsenoside Rg ₃ are in a ratio of 1: 1 to 1: 5. When applied to it was found that excellent ultraviolet protection effect appeared to complete the present invention.

The composition containing the ginsenoside Rh ₂ and the ginsenoside Rg ₃ of the present invention in a ratio of 1: 1 to 1: 5 is superior to the sun protection of the ginsenoside Rh ₂ and the ginsenoside Rg ₃ alone. Effect.

Accordingly, an object of the present invention is to provide a cosmetic composition containing ginsenoside Rh ₂ and ginsenoside Rg ₃ having from 1: 1 to 1: 5 having excellent UV blocking effect.

Still another object of the present invention is to provide a softening longevity, nourishing longevity, massage cream or pack using the composition exhibiting excellent sunscreen effect by continuously applying to the skin.

Hereinafter, the present invention will be described in detail.

Ginsenoside Rh ₂ and Ginsenoside Rg ₃ as an active ingredient according to the present invention, the composition comprising a ratio of 1: 1 to 1: 5 is a cosmetic composition that exhibits excellent sunscreen effect to protect the skin It can be usefully used.

If the ratio of ginsenoside Rh ₂ and ginsenoside Rg ₃ is 1: 1 or more or 1: 5 or less, there is a problem in that the UV blocking effect is not sufficient.

The amount of ginsenoside Rh ₂ and ginsenoside Rg _{3 according} to the present invention applied to the human skin may be appropriately selected according to the condition of the skin, and in general, it is 0.0001 to 10% as a solid content per formulation. It is preferable to apply 1 to 3 times a day.

The composition of the present invention can be applied to the skin prepared by a flexible cosmetic water, nourishing cosmetics, massage cream or pack in a conventional manner.

In the case of producing cosmetics in such a formulation, other ingredients are not particularly limited and can be freely selected.

In addition, it can be used in combination with various substances (or drugs) that are effective for skin whitening, antioxidant, cell activity (anti-aging), moisturizing, skin roughening, skin elasticity improvement and the like.

When ginsenoside Rh ₂ and ginsenoside Rg ₃ which are active ingredients of the composition according to the present invention are prepared in a ratio of 1: 1 to 1: 5 and applied to the skin, the effect is not only excellent, but the experiment described below As evidenced by the results, it has no side effects and can be used safely.

The present invention is explained in more detail by the following examples and experimental examples, but the present invention is not limited in any way by these.

Example 1 Preparation of Ginsenoside Rh ₂

(1) Preparation of Glycolytic Enzyme Liquid

After incubation at 30 ° C for 80 hours by adding Korean bacteria (Nucillus bacteria) to a medium containing 3% wheat flour and 1% ginseng powder, the cells were removed by centrifugation, and the enzyme protein was precipitated with 70% ethyl alcohol solution. The protein was collected, dissolved in 1/10 volume of sodium acetate solution (0.02M, pH 5.0) of the culture solution, and centrifuged to remove impurities to obtain an enzyme solution.

(2) Preparation of Ginsenoside Rh ₂

3 g of ginsenoside Rd was dissolved in 100 ml of acetic acid solution (0.02 M, pH 5.0), 50 ml of the glycolytic enzyme solution prepared in (1) was added, and then reacted at 40 ° C. for 20 hours to prepare ginsenoside F2. It was. Then, 1/3 volume of butanol was added, and saponin was extracted three times, and evaporated to dryness under reduced pressure. Acetic acid was converted to ginsenoside Rh ₂ by hydrolysis of the pull at the 20th carbon position of the ginsenoside F 2 molecule with acetic acid. This was separated by silica gel column separation to prepare 1.2 g of ginsenoside Rh ₂ .

Example 2 Preparation of Ginsenoside Rg ₃

Ginseng irradiated 6 g of ginseng irradiated with 20 ml of 50% acetic acid prepared using a conventional method of separating the extract extracted using ginseng or red ginseng as an alcoholic extraction solvent, heated with stirring at 70 ℃ for 2 hours and then the reaction mixture at room temperature Cooled down. The reaction solution was diluted with 200 ml of distilled water and extracted three times with 100 ml of n-butanol. The combined extracts were washed twice with saturated sodium bicarbonate solution and the n-butanol layer was concentrated under reduced pressure. The reaction mixture was separated by silica gel column chromatography (chloroform: ethanol: water = 100: 30: 10, lower layer) to obtain 2.25 g of ginsenoside Rg ₃ .

[Manufacture example 1 ~ 5] flexible lotion (skin lotion)

A flexible lotion containing ginsenosides Rh ₂ and Rg ₃ of the present invention was prepared in the following component ratios.

                                                               (Unit: weight%)

ingredient Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Comparative Example 1 Comparative Example 2 Ginsenoside Rh ₂ 5.0 4.0 2.5 0.8 0.5 5.0 0 Ginsenoside Rg ₃ 0.5 0.8 2.5 4.0 5.0 0 5.0 1,3-butylene glycol 6.0 6.0 6.0 6.0 6.0 6.0 6.0 Sodium hyaluronate 2.0 2.0 2.0 2.0 2.0 2.0 2.0 glycerin 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Fiji 4000 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Poly Solvent 20 0.5 0.5 0.5 0.5 0.5 0.5 0.5 ethanol 10.0 10.0 10.0 10.0 10.0 10.0 10.0 antiseptic a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount incense a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Sum 100 100 100 100 100 100 100

[Manufacture example 6] Nourishing lotion (milk lotion)

A nourishing lotion containing ginsenosides Rh ₂ and Rg ₃ of the present invention was prepared in the following component ratios.

ingredient Content (% by weight) Ginsenoside Rh ₂ 2.5 Ginsenoside Rg ₃ 2.5 Beeswax 4.0 Poly Solvent 60 1.5 Sorbitan sesquioleate 0.5 Floating paraffin 5.0 Squalane 5.0 glycerin 5.0 Butylene glycol 5.0 Carboxyvinyl Polymer 0.2 Triethanol amine 0.2 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Remaining amount Sum 100

[Manufacture example 7] Massage cream

Massage creams containing ginsenosides Rh ₂ and Rg ₃ of the present invention were prepared in the following component ratios.

ingredient Content (% by weight) Ginsenoside Rh ₂ 0.8 Ginsenoside Rg ₃ 4.0 Beeswax 10.0 Poly Solvent 60 1.5 PIO 60 Hardened Castor Oil 2.0 Sorbitan sesquioleate 1.0 Floating paraffin 40.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Remaining amount Sum 100

Preparation Example 8 Pack

A pack containing ginsenosides Rh ₂ and Rg ₃ of the present invention was prepared at the following component ratios.

ingredient Content (% by weight) Ginsenoside Rh ₂ 4.0 Ginsenoside Rg ₃ 0.8 Polyvinyl alcohol 14.0 Sodium Carboxymethyl Cellulose 0.3 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Nonylphenyl Ether 0.3 Polysorbate 60 0.3 Pigment a very small amount Spices a very small amount Purified water Remaining amount Sum 100

Experimental Example 1 SPF (Ultraviolet Ray Blocking Index) Measurement Test

SPF (ultraviolet ray blocking index) measurement test was performed on ginsenoside Rh ₂ and ginsenoside Rg ₃ using Preparation Examples 1 to 5. Sun Protection Factor (hereinafter referred to as SPF) refers to the generation of MED on the treated skin against the UV energy required to produce minimal erythema on untreated skin (hereinafter referred to as MED). It is defined as the ultraviolet energy required to achieve this, which can vary slightly from race to race.

SPF = minimum erythema of treated skin / minimum erythema of untreated skin

Experimental method was applied to 30 adult men and women with healthy skin using surgical glove to thickness 2mg / ㎠ and width 30㎠ and dried at room temperature for more than 15 minutes to ensure volatilization of the sample. I was. After 18 hours of irradiation with ultraviolet light using a solar simulator, two judges measured the MED value for each person and calculated the SPF value.

Table 1 shows SPF values measured for Preparation Examples 1 to 5 and Comparative Examples 1 and 2 of the present invention.

SPF measurement result Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Comparative Example 1 Comparative Example 2 SPF 8 10 15 17 11 7 5

From the above and the experimental results, it can be seen that Preparation Example 3 and Preparation Example 4 has a very excellent UV blocking effect compared to the Comparative Example. In other words, the group administered with ginsenoside Rh ₂ and ginsenoside Rg ₃ in a ratio of 1: 1 to 1: 5 is much better than the group administered with ginsenoside Rh ₂ alone or ginsenoside Rg ₃ alone. It can be seen that appears.

Experimental Example 2 Safety Test

In order to test the skin safety of the composition of the present invention, a safety test was performed as follows.

3-1 Skin Primary Irritation Test

The samples of Preparation Example 3 and Preparation Example 4 were applied to the skin of the white male rabbit (group 1 10) from which the hair of the dorsal skin was removed so as to have a thickness of 2 mg / cm 2 or more three times a day. Determination was performed with erythema and edema as an indicator by primary irritation rating at 24, 48 and 72 hours after application. As a result, erythema and edema did not appear in all groups, which was judged to be negative.

3-2 Skin Accumulation Irritation Test

The samples of Preparation Example 3 and Preparation Example 4 were applied to the skin of white male rabbits (group 1 10) from which the hair of the dorsal skin was removed so as to have a thickness of 2 mg / cm 2 or more once a day, five times a week. The application was carried out for 2 weeks and the hair removal was carried out on the last application day every week. The determination was carried out on the day of each application and the day after the last application, with erythema and edema as an index by the primary irritation rating method. As a result, all the groups did not show any erythema or edema over 2 weeks, which was judged as negative.

As a result of the skin primary irritation test and the cumulative skin irritation test, it can be seen that the composition of the present invention is very safe for the skin.

Experimental Example 3 Acute Toxicity Test of Ginsenoside Rh ₂ and Ginsenoside Rg ₃

1) Test substance: Ginsenoside Rh ₂ and Ginsenoside Rg ₃

Test animals: Male rats 6 weeks old and male mice 5 weeks old (10 animals / group)

3) Preparation of test substance: The composition was prepared by completely suspending the composition in 1% CMC before administration on the test day.

4) Test Method: After fasting the test animals for 6 hours, the drug was administered orally into the stomach of the test animals using a mouse and rat bulge. Dosage was administered based on body weight immediately prior to dosing and observed death for 14 days.

5) Test Results: As a result of acute toxicity test in rats and mice, LD ₅₀ of the composition of rats and mice was 3g / kg or more.

Acute Toxicity Test Results in Rats and Mice Medication Test animals Test concentration (mg / kg) Tuyeng female animals Social network % Mortality LD ₅₀ Rh ₂ : Rg ₃ = 1: 1 Rat 01883757501,5003,000 101010101010 000000 000000 > 3g / kg Rh ₂ : Rg ₃ = 1: 1 mouse 01883757501,5003,000 101010101010 000000 000000 > 3g / kg Rh ₂ : Rg ₃ = 1: 5 Rat 01883757501,5003,000 101010101010 000000 000000 > 3g / kg Rh ₂ : Rg ₃ = 1: 5 mouse 01883757501,5003,000 101010101010 000000 000000 > 3g / kg

Ginsenosides Rh ₂ and ginsenosides Rg ₃ of the active ingredient according to the present invention contains a 1: 1 to 1: 5 composition exhibits an excellent sunscreen effect than ginsenoside Rh ₂ and ginsenoside Rg ₃ alone .

In addition, there is no side effect on the skin can be applied to the skin prepared by applying cosmetics to the skin for a long time.

Claims (3)

Ginsenoside Rh ₂ and Ginsenoside Rg ₃ A cosmetic composition having a sunscreen effect, characterized in that it is contained in a weight ratio of 1: 1 to 1: 5. According to claim 1, wherein the cosmetic composition is a cosmetic composition, characterized in that prepared in the formulation of the flexible cosmetics, nourishing cosmetics, massage cream or pack. The cosmetic composition according to claim 2, wherein the ginsenoside Rh ₂ and the ginsenoside Rg ₃ are contained in an amount of 0.0001 to 10% (wt%) as a solid content per formulation.