KR100472763B1 - A pollution soil disposal mehtod using Phytoremediation - Google Patents
A pollution soil disposal mehtod using Phytoremediation Download PDFInfo
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- KR100472763B1 KR100472763B1 KR10-2002-0027255A KR20020027255A KR100472763B1 KR 100472763 B1 KR100472763 B1 KR 100472763B1 KR 20020027255 A KR20020027255 A KR 20020027255A KR 100472763 B1 KR100472763 B1 KR 100472763B1
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- 239000002689 soil Substances 0.000 title claims abstract description 93
- 244000005700 microbiome Species 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 23
- 241000196324 Embryophyta Species 0.000 claims abstract description 18
- 241000219823 Medicago Species 0.000 claims abstract description 12
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 241000588625 Acinetobacter sp. Species 0.000 claims abstract description 4
- 241000589774 Pseudomonas sp. Species 0.000 claims abstract description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 4
- 239000011707 mineral Substances 0.000 claims abstract description 4
- 230000007935 neutral effect Effects 0.000 claims abstract description 4
- 239000005416 organic matter Substances 0.000 claims abstract description 4
- 150000003839 salts Chemical class 0.000 claims abstract description 4
- BBEAQIROQSPTKN-UHFFFAOYSA-N pyrene Chemical compound C1=CC=C2C=CC3=CC=CC4=CC=C1C2=C43 BBEAQIROQSPTKN-UHFFFAOYSA-N 0.000 abstract description 31
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthrene Natural products C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 abstract description 17
- 238000000746 purification Methods 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 10
- 230000000813 microbial effect Effects 0.000 description 5
- 239000003921 oil Substances 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 125000005581 pyrene group Chemical group 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 150000002118 epoxides Chemical class 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 102000008109 Mixed Function Oxygenases Human genes 0.000 description 1
- 108010074633 Mixed Function Oxygenases Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000006303 photolysis reaction Methods 0.000 description 1
- 230000015843 photosynthesis, light reaction Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000004053 quinones Chemical class 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02B—HYDRAULIC ENGINEERING
- E02B3/00—Engineering works in connection with control or use of streams, rivers, coasts, or other marine sites; Sealings or joints for engineering works in general
- E02B3/04—Structures or apparatus for, or methods of, protecting banks, coasts, or harbours
- E02B3/06—Moles; Piers; Quays; Quay walls; Groynes; Breakwaters ; Wave dissipating walls; Quay equipment
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- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Ocean & Marine Engineering (AREA)
- Mechanical Engineering (AREA)
- Civil Engineering (AREA)
- Structural Engineering (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
본 발명은 식물복원기술을 이용한 오염토양 처리방법에 관한 것으로서, 더욱 상세하게는 토양에 배양한 토양미생물 및 패각분말을 첨가하고 수분을 40∼60부피%를 유지하여 토양내의 pyrene의 농도를 낮게하는 것으로 식물복원기술을 이용한 오염토양 처리방법에 관한 것이다.The present invention relates to a method for treating contaminated soil using plant restoration technology, and more specifically, adding soil microorganisms and shell powder cultured to soil and maintaining 40 to 60% by volume of water to lower the concentration of pyrene in the soil. The present invention relates to a method for treating contaminated soil using plant restoration technology.
본 발명의 목적은 식물에 의하여 유류로 오염된 토양의 pyrene을 처리하는데 있어서 rhizosph토양의 미생물의 밀도와 토양의 상태가 pyrene의 처리효율에 크게 영향을 미칠 수 있을 것이므로, 토양미생물을 배양하여 rhizosphere 지역에 접종하므로서 미생물의 밀도를 높혀서 phytoremediation의 상승제로 사용할 수 있는 식물복원기술을 이용한 오염토양 처리방법을 제공함에 있다.It is an object of the present invention to treat pyrene in soil contaminated with oil by plants, so the density and soil condition of rhizosph soil microorganisms can greatly affect the treatment efficiency of pyrene, so that the soil microorganisms are cultured in the rhizosphere region. By inoculating on the microorganisms to increase the density of the phytoremediation to provide a soil treatment method using plant restoration technology that can be used as a synergist.
또한, 본 발명의 또 다른 목적은 상기와 같은 pyrene의 phytoremediation에서 rhizosphere 토양에 패각을 첨가하여 phytoremediation에 의한 토양 정화를 효과적으로 촉진시켜 처리효율을 증진할 수 있는 식물복원기술을 이용한 오염토양 처리방법을 제공함에 있다.In addition, another object of the present invention is to provide a contaminated soil treatment method using a plant restoration technology that can increase the treatment efficiency by effectively promoting the soil purification by phytoremediation by adding a shell to the rhizosphere soil in the phytoremediation of pyrene as described above. Is in.
상기의 목적을 달성하기 위한 본 발명의 구성은, 유기물을 분해에 사용되는 슈드모나스 속(Pseudomonas sp.), 아시네토박터 속(Acinetobacter sp.) 균주 중 어느 하나를 선택하여 통상의 mineral salts medium 배지에 중성, 중온조건에서 진탕 배양한 토양미생물을 이용한 오염토양 처리방법에 있어서, 1kg의 토양당 상기 토양미생물의 양을 0.01 ∼ 1g 첨가하고 알팔파를 심어 실온에서 수분을 40∼60부피%로 유지하는 것을 특징으로 한다.The composition of the present invention for achieving the above object is, by selecting any one of the strain Pseudomonas sp., Acinetobacter sp. Used to decompose organic matter, mineral salts medium medium In the contaminated soil treatment method using soil microorganisms shaken and cultured in neutral and moderate temperature conditions, 0.01 to 1 g of the soil microorganism is added per 1 kg of soil, and alfalfa is planted to maintain water at room temperature to 40 to 60% by volume. It is characterized by.
또한 바람직하게는, 상기 1kg의 토양당 패각분말 5 ∼ 50g을 더 첨가한 것을 특징으로 한다.Also preferably, 5 to 50 g of shell powder per 1 kg of soil is further added.
Description
본 발명은 식물복원기술을 이용한 오염토양 처리방법에 관한 것으로서, 더욱 상세하게는 토양에 배양한 토양미생물 및 패각분말을 첨가하고 수분을 40∼60부피%를 유지하여 토양내의 pyrene의 농도를 낮게하는 것으로 식물복원기술을 이용한 오염토양 처리방법에 관한 것이다.The present invention relates to a method for treating contaminated soil using plant restoration technology, and more specifically, adding soil microorganisms and shell powder cultured to soil and maintaining 40 to 60% by volume of water to lower the concentration of pyrene in the soil. The present invention relates to a method for treating contaminated soil using plant restoration technology.
특히 유류에 포함된 PAHs(poly aromatic hydrocarbons)중에서 pyrene은 생물학적 분해능이 비교적 낮은 유해화학물질이다. PAHs의 독성효과는 호흡, 피부접촉 그리고 소화기에 의해 신체에 흡수되어 나타난다. PAHs는 eucaryotic degredation pathway를 거쳐 대사되며 화합물은 초기에 liver monooxygenases에 의해서 arene oxides(epoxides), dihydrodiols, phenols 그리고 quinones로 전환된다. 특히 Epoxides는 유전물질과 결합하여 종양의 출발점이 된다.Among the polyaromatic hydrocarbons (PAHs) contained in oils, pyrene is a hazardous chemical with a relatively low biological resolution. The toxic effects of PAHs are absorbed by the body by breathing, skin contact and digestive system. PAHs are metabolized via the eucaryotic degredation pathway and compounds are initially converted to arene oxides (epoxides), dihydrodiols, phenols and quinones by liver monooxygenases. Epoxides, in particular, bind to genetic material and become the starting point for tumors.
오염된 환경에서 PAHs를 제거하기 위한 과정은 휘발, 침출 그리고 광분해와 같은 메카니즘이 효과적일 수도 있지만 주요과정은 미생물에 의한 분해이며 bioremediation은 유망한 정화방법중의 하나이다.Processes for removing PAHs in contaminated environments may be effective mechanisms such as volatilization, leaching and photolysis, but the main process is microbial degradation and bioremediation is one of the promising purification methods.
최근에 식물을 이용한 PAHs의 정화방법은 넓은 범위에 걸쳐서 저농도로 오염되어 있는 토양인 경우에 적합한 것으로 주목받고 있다. 식물에 의한 토양중의 PAHs 제거의 효과적인 결과는 두가지 측면으로 고려할 수 있다.Recently, the method of purifying PAHs using plants has been attracting attention in the case of soils contaminated with low concentration over a wide range. Effective results of PAHs removal from soil by plants can be considered in two aspects.
첫 번째, 식물뿌리는 유기오염물질을 흡수, 축적하여 대사작용에 이용하거나 휘발시킨다.First, plant roots absorb and accumulate organic pollutants and use them for metabolism or volatilize them.
두 번째는 식물뿌리 주위의 rhizosphre microflora가 오염물질의 미생물 분해를 가속화 시킨다. Rhizosphre 토양에서 미생물의 높은 밀도와 다양성 때문에 후자가 PAHs의 미생물 분해를 위한 중요한 인자로 간주된다. Rhizosphre effect는 non-rhizosphre 토양에서의 미생물의 수 대 rhizosphre토양에서의 미생물의 수의 비로 보통 5∼20범위에 있다.Second, rhizosphre microflora around the plant roots accelerates microbial degradation of contaminants. Rhizosphre The latter is considered an important factor for the microbial degradation of PAHs due to the high density and diversity of microorganisms in soil. Rhizosphre effect is the ratio of the number of microorganisms in non-rhizosphre soils to the number of microorganisms in rhizosphre soils.
즉, 식물을 이용한 유류로 오염된 토양의 정화법등은 Ecological Engineering 또는 Biosource Technology등을 통해 널리 알려져 있다. 그러나 phytoremediation법은 처리속도가 느리고 처리기간이 길다.That is, the method of purifying soil contaminated with oil using plants is widely known through Ecological Engineering or Biosource Technology. However, the phytoremediation method is slow in processing and long in processing.
그러므로, 특히 유류으로 오염된 토양의 phytoremediation법에 의한 정화법에서 처리속도를 가속화시켜 처리기간과 효율을 증진시키는 기술이 절실하게 요구된다고 할 것이나 지금까지는 유류에 포함된 생물학적으로 난분해성인 PAHs 때문에 쉽게 정화할수 없는 단점을 해결할 수 있는 방안을 찾지 못하고 있는 실정이다.Therefore, especially in the phytoremediation of oil-contaminated soils, there is an urgent need for technology that accelerates the treatment speed and improves the treatment time and efficiency, but so far it is easily purified because of the biodegradable PAHs contained in the oil. There is no way to find a solution to the disadvantages.
본 발명은 상기의 제반 문제점을 해결하기 위해 창안된 것으로서, 본 발명의 목적은 식물에 의하여 유류로 오염된 토양의 pyrene을 처리하는데 있어서 rhizosph토양의 미생물의 밀도와 토양의 상태가 pyrene의 처리효율에 크게 영향을 미칠 수 있을 것이므로, 토양미생물을 배양하여 rhizosphere 지역에 접종하므로서 미생물의 밀도를 높혀서 phytoremediation의 상승제로 사용할 수 있는 식물복원기술을 이용한 오염토양 처리방법을 제공함에 있다.The present invention was devised to solve the above problems, and an object of the present invention is to treat pyrene in soil contaminated with oil by plants, and the density of microorganisms in soil and soil condition is related to pyrene treatment efficiency. In order to increase the microbial density by inoculating the rhizosphere by cultivating soil microorganisms, the soil soil treatment method using plant restoration technology can be used as a synergist of phytoremediation.
또한, 본 발명의 또 다른 목적은 상기와 같은 pyrene의 phytoremediation에서 rhizosphere 토양에 패각을 첨가하여 phytoremediation에 의한 토양 정화를 효과적으로 촉진시켜 처리효율을 증진할 수 있는 식물복원기술을 이용한 오염토양 처리방법을 제공함에 있다.In addition, another object of the present invention is to provide a contaminated soil treatment method using a plant restoration technology that can increase the treatment efficiency by effectively promoting the soil purification by phytoremediation by adding a shell to the rhizosphere soil in the phytoremediation of pyrene as described above. Is in.
상기의 목적을 달성하기 위한 본 발명의 구성은, 유기물을 분해에 사용되는 슈드모나스 속(Pseudomonas sp.), 아시네토박터 속(Acinetobacter sp.) 균주 중 어느 하나를 선택하여 통상의 mineral salts medium 배지에 중성, 중온조건에서 진탕 배양한 토양미생물을 이용한 오염토양 처리방법에 있어서, 1kg의 토양당 상기 토양미생물의 양을 0.01 ∼ 1g 첨가하고 알팔파를 심어 실온에서 수분을 40∼60부피%로 유지하는 것을 특징으로 한다.The composition of the present invention for achieving the above object is, by selecting any one of the strain Pseudomonas sp., Acinetobacter sp. Used to decompose organic matter, mineral salts medium medium In the contaminated soil treatment method using soil microorganisms shaken and cultured in neutral and moderate temperature conditions, 0.01 to 1 g of the soil microorganism is added per 1 kg of soil, and alfalfa is planted to maintain water at room temperature to 40 to 60% by volume. It is characterized by.
또한 바람직하게는, 1kg의 토양당 패각분말 5 ∼ 50g을 더 첨가한 것을 특징으로 한다.Also preferably, 5 to 50 g of shell powder per 1 kg soil is further added.
본 발명의 방법을 구체적으로 설명하면 다음과 같다.The method of the present invention will be described in detail as follows.
토양을 수거하는 단계, 토양은 사용하기전에 1/4＂체를 사용하여 처리하고 토양의 특성을 분석한다. 토양의 특성은 표 1과 2에 나타낸바와 같다. 토양중의 PAHs 농도를 가각 100mg/kg으로 조절하기 위하여 4000mg pyrene/L acetone 용액중에서 25mL를 취하여 1kg의 토양에 분무시켜 혼합하였다. 식물은 알팔파를 사용하였고 오염된 토양으로만 구성된 반응기(화분), 오염된 토양과 알팔파로 구성된 반응기, 오염된 토양과 알팔파로 구성된 반응기에 배양된 토양미생물을 다양한 밀도로 접종한 반응기, 오염된 토양과 알팔파로 구성된 반응기에 다양한 농도의 패각을 첨가한 반응기 그리고 오염된 토양과 알팔파로 구성된 반응기에 배양된 토양미생물을 접종하고 패각을 첨가한 반응기를 구성하여 pyrene으로 오염된 토양을 처리할 수 있도록 하였다.At the stage of harvesting the soil, the soil is treated with a 1 / 4-inch sieve prior to use and the soil is characterized. Soil characteristics are shown in Tables 1 and 2. In order to adjust the concentration of PAHs in the soil to 100mg / kg each, 25mL in 4000mg pyrene / L acetone solution was sprayed and mixed in 1kg soil. Plants used alfalfa, reactors consisting only of contaminated soil (pots), reactors consisting of contaminated soils and alfalfa, reactors inoculated at different densities of soil microorganisms in contaminated soils and reactors consisting of alfalfa, contaminated soil A reactor consisting of shells and alfalfa was inoculated with shells of various concentrations, and the inoculated soil microorganisms were inoculated with contaminated soils and alfalfa reactors and shells were added to treat the soil contaminated with pyrene. .
본 발명에 있어서 phytoremediation은 온도와 수분에 의하여 영향을 받을 수 있으므로 온도는 실온을 유지하였고 토양의 수분은 40-30%로 유지되도록 하였으며 실험은 150일동안 수행되었으며 샘플의 분석은 한번에 각 토양 종류별로 3개의 반응기를 취해서 15일 간격으로 하였다. In the present invention, the phytoremediation may be affected by temperature and moisture, so that the temperature was maintained at room temperature and the soil moisture was maintained at 40-30%. The experiment was performed for 150 days and the analysis of the sample was performed for each soil type at a time. Three reactors were taken and placed at 15 day intervals.
또한 다음 표 3에 도시한 바와 같은 토양미생물 배양액으로 배양한 토양미생물을 접종할때 토양미생물의 양과 패각의 양등의 조절이 매우 중요하였다.In addition, when inoculating the soil microorganisms incubated with the soil microbial culture as shown in Table 3, the control of the amount of the soil microorganisms and the amount of the shell was very important.
이처럼 본 발명에 의해 토양미생물을 phytoremediation 공정에 첨가하면 rhizosphere 토양속의 미생물밀도의 증가에 기인하여 처리효율이 증가하고 처리기간이 단축되었으며, 또한 패각분말을 첨가한 rhizosphere 토양의 처리 효율도 증가하고 처리기간도 단축되는 것을 확인 할 수 있었다. As such, when the soil microorganism is added to the phytoremediation process according to the present invention, the treatment efficiency is increased and the treatment period is shortened due to the increase of the microbial density in the rhizosphere soil. Also, the treatment efficiency of the rhizosphere soil containing shell powder is increased and the treatment period is increased. It could also be confirmed that it is shortened.
이는 패각의 주성분인 탄산칼슘이 미생물의 성장에 필요한 무기성분(영양분)이 되어 미생물의 성장을 촉진하는 것으로 판단된다.It is believed that calcium carbonate, which is a major component of the shell, becomes an inorganic component (nutrient) necessary for the growth of microorganisms and promotes the growth of microorganisms.
토양미생물과 패각을 동시에 첨가하면 상승효과가 나타나 rhizosphere 토양의 처리효율과 처리기간이 각각을 첨가할 때 보다 향상되었다.Simultaneous addition of soil microorganisms and shells produced synergistic effects, which improved the treatment efficiency and treatment duration of rhizosphere soil.
또한 본 발명에 의해 종래의 phytoremediation과 토양미생물과 패각을 첨가한 개선된 phytoremediation의 처리효과 정도를 알아보기 위하여 시간에 따라 각 구성된 반응기의 토양을 채취하여 토양속의 pyrene을 용매로 추출하여 가스크로마토그래프로 농도를 측정하여 처리효율과 처리기간을 확인 할 수 있었다.한편, 상기 토양미생물은 유기물을 분해에 사용되는 슈드모나스 속(Pseudomonas sp.), 아시네토박터 속(Acinetobacter sp.) 균주 중 어느 하나를 선택하여 통상의 mineral salts medium 배지에 중성, 중온조건에서 진탕 배양된 공지의 토양미생물이며, 상기 미생물 균종은 토양에서 얻은 미생물이고, 그 배양방법은 오염된 토양의 복원기술에 주로 사용되어 왔던 기술이다.In addition, the present invention extracts the soil of each configured reactor with time to extract the phytoremediation of the conventional phytoremediation and improved phytoremediation with the addition of soil microorganisms and shells, and extracts the pyrene in the soil as a solvent to gas chromatography. By measuring the concentration, it was possible to confirm the treatment efficiency and the treatment period. Meanwhile, the soil microorganism was selected from strains of Pseudomonas sp. And Acinetobacter sp., Which are used to decompose organic matter. It is a known soil microorganism selected and cultured in normal mineral salts medium medium under shaking and neutral conditions, and the microbial species is a microorganism obtained from soil, and the culture method is a technique that has been mainly used for the restoration of contaminated soil. .
이하 실시예에 의해 상세히 설명하기로 한다.It will be described in detail by the following examples.
실시예 1Example 1
1kg의 토양을 반응기에 넣고 알팔파를 심어 실온으로 유지하고 수분을 40∼60%로 유지하여 시간에 따른 pyrene의 분해정도를 확인한다.Put 1kg soil into the reactor and plant alfalfa to keep it at room temperature and keep the moisture at 40 ~ 60% to check the decomposition of pyrene over time.
실시예 2Example 2
1kg의 토양에 배양한 토양미생물의 양을 0.01 ∼ 1g 첨가하여 반응기에 넣고 알팔파를 심어 실온으로 유지하고 수분을 40∼60%로 유지하여 시간에 따른 pyrene의 분해정도를 확인한다.Add 0.01 ~ 1g of the amount of soil microorganisms cultured in 1kg of soil, put it in the reactor, plant alfalfa, keep it at room temperature, and keep moisture at 40 ~ 60% to check the decomposition of pyrene over time.
실시예 3 Example 3
1kg의 토양에 패각분말 5 ∼ 50g 첨가하여 반응기에 넣고 알팔파를 심어 실온으로 유지하고 수분을 40∼60%로 유지하여 시간에 따른 pyrene의 분해정도를 확인한다.Add 5-50 g of shell powder to 1 kg of soil, put it in the reactor, plant alfalfa and keep it at room temperature, and keep the moisture at 40-60% to check the decomposition of pyrene over time.
실시예 4Example 4
1kg의 토양에 배양한 토양미생물의 양을 0.01 ∼ 1g 첨가하고, 패각분말 5 ∼ 50g 첨가하여 반응기에 넣고 알팔파를 심어 실온으로 유지하고 수분을 40∼60%로 유지하여 시간에 따른 pyrene의 분해정도를 확인한다.Add 0.01-1 g of soil microorganism cultured to 1 kg soil, add 5-50 g of shell powder, put it in the reactor, plant alfalfa, keep it at room temperature and maintain moisture at 40-60%. Check.
상기 실시예 1 내지 4의 pyrene의 농도를 표 4에 나타내었다.The concentration of pyrene of Examples 1 to 4 is shown in Table 4.
시험결과 본 발명에 의해 처리되어 pyrene의 농도가 낮아진 토양의 처리효율은 5%에서 25%로 증가하였고 따라서 처리기간도 단축 시킬수 있었다.As a result of the test, the treatment efficiency of the soil treated with the present invention, which lowered the concentration of pyrene, increased from 5% to 25%, thus shortening the treatment period.
이는 본 발명에 의해 phytoremediation으로 유류로 오염된 토양을 우수한 효율로 처리 할 수 있음을 의미하는 것이다.This means that the soil contaminated with oil by phytoremediation can be treated with excellent efficiency.
상술한 바와 같이 본 발명은 종래 일반폐기물로 분류되어 폐기되던 패각을 토양오염처리를 위한 phytoremediat 촉진제로 사용함으로서 재활용자원으로 활용할 수 있고 고유의 토양미생물을 배양하여 함께 phytoremediation에 적용함으로서 오염된 토양을 저비용으로 효과적으로 처리할 수 있는 방안이므로 국가의 경제에도 도움이 될 수 있는 매우 유용한 발명인 것이다.As described above, the present invention can be utilized as a recycling resource by using the shells that have been classified and disposed of as conventional wastes as phytoremediat accelerators for soil pollution treatment, and by cultivating unique soil microorganisms and applying them to phytoremediation, low-cost polluted soils It is a very useful invention that can help the economy of the country because it can be effectively dealt with.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5286140A (en) * | 1992-12-17 | 1994-02-15 | Petro Environmental Technologies, Inc. | Bioremediation systems and methods |
| JPH1199380A (en) * | 1997-09-26 | 1999-04-13 | Ohbayashi Corp | Method of decomposing and removing oil polluted soil by microorganism |
| JPH11333442A (en) * | 1998-05-26 | 1999-12-07 | Toko Corp | Method for decontaninating contaminated soil |
| KR20000034035A (en) * | 1998-11-27 | 2000-06-15 | 구본탁 | Biological Purification of Oil Polluted Soils Using Petroleum Hydrolyzed Microbial Agents |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5286140A (en) * | 1992-12-17 | 1994-02-15 | Petro Environmental Technologies, Inc. | Bioremediation systems and methods |
| JPH1199380A (en) * | 1997-09-26 | 1999-04-13 | Ohbayashi Corp | Method of decomposing and removing oil polluted soil by microorganism |
| JPH11333442A (en) * | 1998-05-26 | 1999-12-07 | Toko Corp | Method for decontaninating contaminated soil |
| KR20000034035A (en) * | 1998-11-27 | 2000-06-15 | 구본탁 | Biological Purification of Oil Polluted Soils Using Petroleum Hydrolyzed Microbial Agents |
Non-Patent Citations (2)
| Title |
|---|
| Pure Appl. Chem., Vol.71, No.1, pp.161-171, 1999 * |
| 한국생물공학회, 학술대회논문집, 2001 * |
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