KR100459265B1 - Culture media containing grinded jellyfish - Google Patents

Abstract

The present invention relates to a culture medium comprising the jellyfish pulverized product as an active ingredient and a method for producing the same. Since the medium of the present invention regulates the growth and metabolism of the microorganism to increase the viability and activity of the microorganism, it can be usefully used for culturing microorganisms and tissue culture of animals and plants for the preparation of microbial pesticide raw materials and preparations.

Description

Culture medium containing jellyfish pulverization {Culture media containing grinded jellyfish}

The present invention relates to a culture medium comprising the jellyfish pulverized product as an active ingredient and a method for producing the same.

In general, the medium contains a nutrient required by the culture to culture microorganisms or tissues of animals and plants as a main component, and is prepared by adding various inorganic compounds or organic compounds depending on the purpose of culture.

Jellyfish belong to the animal taxonomy of cryptic tonic or tonic animal portals. Jellyfish are largely hydroclamp (ClassHydrozoa) And Jellyfish River (ClassScyphozoaAre classified as). Jellyfish, belonging to the Heathentulum, are hydromedus, a floating larvae of the life cycles of the various Heathoptera lifeHydromedusae) And about Paris (SiphonophoresCan be divided into Most jellyfish are umbrella-shaped and have many tentacles around them. The jellyfish's body looks like a clear jelly, and most of it's water is very fragile. It is per 100 g of jellyfish It contains nutrients of 3.3 g protein, 1.7 g ash, 23 mg calcium, 10 mg phosphorus, 5 mg iron and 0.8 g fat. Therefore, it is widely used for food after processing, and it is known that there is no problem in safety.

Meanwhile, methods for controlling diseases and pests of crops include physical methods for improving the cultivation environment, chemical methods using fungicides, and microbiological methods using microorganisms. However, physical control alone cannot be expected to have a great effect, and chemical control has not only significantly reduced the control effect due to the emergence of multi-drug resistance bacteria, but also residual toxicity and It causes serious environmental pollution caused by residual pesticides. As an alternative, the research on microbiological control has been actively conducted in recent years, and attention has been paid to research on microbial pesticides for environmentally friendly agriculture.

As microorganisms for the disinfectant of the present microbial pesticides, bacteria include Bacillus (Bacillus sp. BS555, KCTC 8821P ), Bacillus poly Rev. (Bacillus polymoxa B36, FERM BP- 6068), Pseudomonas Oreo Pacific Enschede (Pseudomonas aureofaciens B5, FERM BP-6067 ), Enterobacter cloacae B51 (FERM BP-6069), Bom1 (St. KFCC 10992) and Bom2 (KFCC 10993) in Streptomyces, an actinomycetes that secrete antifungal substances. Also, as microorganisms having antagonistic ability of the pathogenic fungus, alkali jeneseu par faecalis (Alcaligenes faecalis KL1179, KCTC 8914P), Pseudomonas ah rugi labor (Pseudomonas aeruginosa KL1121, KCTC 8915P), Pseudomonas Oran thienyl car (Pseudomonas aurantiaca KL1326, KCTC 8916P) and Psedomonas fluorescens . In addition, bacteria having an antagonistic ability against Actinomycetes Streptomyces genus include Psedomonas fluorescens biovar 1, and fungi include Trichoderma harzianum and Penicillium sp. ), Aspergillus sp ., And Acremonium Strictium BCP ( Acremonium strictum, KCTC 0639BP).

As described above, a number of attempts have recently been made to select novel microorganisms having antagonistic ability against pathogens and to prepare microbial preparations using such microorganisms. In order to prepare a microbial preparation, it is necessary to mass-produce microorganisms with antagonistic ability. As a result, some microorganisms grow in chains (groups) in the process of culturing the microorganisms, so the viability and activity of the microorganisms are drastically decreased. . In addition, growth inhibition by gas and metabolic products produced in-house may occur, resulting in great difficulty in preparing microbial preparations. Therefore, in recent years, in order to easily manufacture microbial preparations, many attempts have been made to suppress or easily recover chain formation and fermentation metabolites (gas, etc.) when culturing microorganisms, but an effective method has not been developed yet. to be.

Therefore, the present inventors prepared a culture medium by adding jellyfish pulverized to a general medium for cultivation, and the microorganisms grown in the medium not only increased viability but also increased metabolic products, and increased stability after formulation. The present invention was completed by confirming.

An object of the present invention is to reduce the addition of nutritional sources and trace components by preparing a culture medium containing jellyfish pulverized marine organisms, and also by using a medium in which jellyfish crushed is added to a general medium component. To provide culture and stable material production.

FIG. 1 is a graph measuring the growth of Bacillus subtilis KBC1010 in a medium having different addition rates of jellyfish pulverization for 24 hours.

Figure 2a is a graph measuring the dry cell mass (g / ℓ) of the cultured strain after culturing Streptomyces natalensis for 5 days in a medium with a different rate of jellyfish pulverization.

Figure 2b is a graph measuring the dry cell mass (g / ℓ) of the culture strain after culturing Pseudozyma flocculosa in a medium of varying the rate of jellyfish pulverization ( Pseudozyma flocculosa ) for 5 days.

Figure 2c is a graph measuring the dry cell mass (g / ℓ) of the cultured strains after incubating Ampelomyces quisqualis for 5 days in a medium with a different rate of jellyfish pulverization.

FIG. 3 is a micrograph showing the chain formation inhibition of Bacillus subtilis KBC1010 cultured in nutrient medium ( A ) and added medium ( B ) of 10% by weight jellyfish pulverization.

FIG. 4 shows Natamycin production (g / l) of Streptomyces natalensis grown in nutrient medium ( A ) and medium ( B ) to which 10% by weight of jellyfish pulverization was added. It is a graph.

5 is a graph measuring total lipid content (dry weight%) of Pseudozyma flocculosa grown in nutrient medium ( A ) and medium ( B ) to which 10% by weight of jellyfish pulverization was added.

FIG. 6 shows Ralstonia solanacearum , a foot blight pathogen of Bacillus subtilis KBC1121 grown in medium ( A ) and nutrient medium ( B ) and 10% by weight of jellyfish pulverized. This is a picture of a petri dish looking at the control effect on).

In order to achieve the above object, the present invention provides a culture medium comprising the jellyfish pulverized product as an active ingredient.

In addition, the present invention (1) washing the jellyfish; (2) pulverizing and dehydrating the washed jellyfish of step 1 to obtain a jellyfish pulverized product; And (3) adding the jellyfish pulverized product obtained in step 2 to the medium.

Hereinafter, the present invention will be described in detail.

The present invention is to provide a culture medium comprising the jellyfish pulverized product as an active ingredient.

The jellyfish of the present invention is not particularly limited, but is preferably selected from the group consisting of remission fly neck, cruciform jellyfish neck, cubic jellyfish neck, near-mouth jellyfish neck, and balloon jellyfish neck, and more preferably, balloon jellyfish neck. In a preferred embodiment of the present invention, a water jellyfish, which is an instrumental jellyfish tree distributed in Korea near the sea, was used. Jellyfish's main components are water and protein (mainly gelatin) and mucus (mucin). When the water content is 70%, the nutritional analysis table of jellyfish is shown in Table 1.

Jellyfish Nutrition Facts (100g, 70% Moisture) carbohydrate 3.3 g calcium 325 mg Fat 0.5 g iron content 8 mg protein 11.3 g sign 70 mg Ash 19.5 g vitamin a very small amount

The water content of the jellyfish pulverized product of the present invention is preferably 50 to 70% in consideration of the role of the nitrogen source and the carbon source of the culture medium and the effect on the physical properties (crushing and mixing) when added to the medium, but not necessarily limited thereto. No.

The microorganism culture medium of the present invention is a jellyfish pulverization in place of the auxiliary trace component in the component for general microbial growth, the amount of jellyfish pulverization used is not particularly limited, it is preferred to add 1 to 90% by weight More preferably, 2 to 20% by weight, most preferably 5 to 15% by weight (see FIGS . 1 and 2 ).

The medium of the present invention provides a medium for the purpose of culturing microorganisms. In this case, the microorganism that can grow in containing the jellyfish pulverized product of the present invention the medium is not particularly limited, Bacillus (Bacillus) genus Lactobacillus bacteria (Lactobacillus) genus Pseudomonas (Pseudomonas) in the alkali jeneseu (Alcaligenes), A Genus Enterobacter , Genus Streptomyces , Genus Ampelomyces , Genus Pseudozyma , Genus Trichoderma , Genus Aspergillus, Genus Penicillium Penicillium) in and Acre monitor Titanium (Acremonium) are preferably selected from the group consisting of genus Bacillus (Bacillus) genus Lactobacillus bacteria (Lactobacillus) genus Streptomyces (Streptomyces), An arm Tupelo My process (Ampelomyces) in And Pseudozyma genus. At this time, the microorganism is from the group consisting of Bacillus subtilis , Streptomyces natalensis , Ampelomyces quisqualis and Pseudozyma flocculosa It is preferred to be selected, most preferably Bacillus subtilis .

In addition, the medium of the present invention provides a medium for the purpose of tissue culture of plants or animals, and may be prepared by adding various inorganic compounds or organic compounds according to the purpose of culture.

The microorganisms cultured in the medium containing the jellyfish pulverized product of the present invention not only increase antimicrobial activity but also stability to excipients compared to microorganisms cultured in a general medium (see Table 3 and Table 5 ). Therefore, in the medium containing the jellyfish of the present invention can produce a large amount of microorganisms with excellent antimicrobial activity, it can be usefully applied in the production of microbial pesticides and microbial preparations using the cultured microorganisms.

Specifically, in order to prepare a microbial preparation, it is necessary to mass-produce a microorganism having high antimicrobial activity, which is an essential component of the microbial preparation.In the case of culturing microorganisms in a commonly used medium, fermentation odors and chains generated during fermentation of microorganisms Formation and metabolic products reduce the antimicrobial activity of the microorganisms. However, when the microorganism is cultured in the medium containing the jellyfish of the present invention, not only the chain formation is suppressed, but also the antimicrobial activity of the microorganism can be continuously maintained, so that the microorganism preparation having high activity can be used ( Fig. 6 ).

In addition, the present invention (1) washing the jellyfish; (2) pulverizing and dehydrating the washed jellyfish of step 1 to obtain a jellyfish pulverized product; And (3) adding the jellyfish pulverized product obtained in step 2 to the culture medium.

Step 1 of the present invention is to wash the jellyfish. The jellyfish species of the present invention is not particularly limited, but is preferably selected from the group consisting of remission fly neck, cruciform jellyfish neck, cubic jellyfish neck, near-mouth jellyfish neck, and balloon jellyfish neck, and more preferably, balloon jellyfish neck. In a preferred embodiment of the present invention, the water jellyfish, which is an instrumental jellyfish neck distributed in the near sea of Korea, was used. Thereafter, the jellyfish was removed using salt and alum to remove foreign substances and seawater from the body fluid, and then washed with clean fresh water to completely remove salts.

Step 2 of the present invention is a step of obtaining a jellyfish pulverized by grinding and dehydrating the washed jellyfish of step 1. In a preferred embodiment of the present invention, the jellyfish pulverized product was adjusted by using a natural method or a dehydrator, the moisture content of the jellyfish pulverized is preferably 5 to 98%, more preferably 50 to 70%. .

Step 3 of the present invention is a step of adding the jellyfish pulverized product obtained in step 2 to the culture medium. At this time, the jellyfish pulverized may be added at 0.1 to 99.9% by weight, preferably added to 2 to 20% by weight, more preferably added to 5 to 15% by weight. There is no restriction | limiting in the kind of culture medium to which the jellyfish grind | pulverized product of this invention is added.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Example 1 Preparation of Medium Containing Jellyfish Crushed

The present inventors pretreated to use jellyfish as a medium, and then prepared the culture medium of the present invention using the pretreated jellyfish.

Specifically, after trapping the jellyfish inhabiting the ocean, the salt and alum were treated to separate foreign bodies and seawater attached to the outside of the body and washed with fresh water for 1 hour to completely remove the salt. After that, the washed jellyfish were ground and homogenized (Avestin A-001 Homogenizer), and the water content was 5-15%, 15-30%, 30-50%, 50-70% and 70-98%, respectively. Pretreated by centrifugal dehydration as possible, and then stored frozen. The adjustment of the water content affects the amount of jellyfish pulverized product during the production of the medium, and if the moisture content is high, the solid content is relatively low, so that the amount of jellyfish pulverized should be proportionally increased. Preference is given to jellyfish mills having a water content adjusted in the range of 50 to 70%.

Jellyfish Moisture Table Water content Solid content Moisture content / solid content 90 10 9 70 30 2.33 50 50 One 30 70 0.43 20 80 0.25 10 90 0.11

Thereafter, the jellyfish pulverized pretreatment to a water content of 67% was added to the nutrient medium (medium medium containing 3 g of beef extract and 4 g of peptone) in distilled water to adjust the final volume to 1 L, respectively. , 15, 20, 30, 50, 75, 90 and 100% by weight was added to prepare a culture medium.

Example 2 Growth Analysis of Microorganisms According to the Amount of Jellyfish Crushed

The present inventors cultured microorganisms using the medium prepared in Example 1, and analyzed the growth of microorganisms according to the amount of jellyfish pulverized products.

Specifically, Bacillus subtilis KBC1010 (KCTC 10304), Streptomyces natalensis (KCCM 40720), Pseudozyma flocculosa ( Pseudozyma flocculosa ) (JCM 10321 ), Amfelomyces Ampelomyces quisqualis (KCTC 8940P) was inoculated into nutrient medium to which jellyfish crushes were added, respectively. After culturing at 30 ° C., the absorbance (550 nm) and dry cell mass were measured to analyze the degree of cell proliferation. At this time, the dry cell weight was measured by the dry weight by collecting the culture, filtration or centrifugation, cell separation, washing and drying sufficiently at 105 ℃ according to a conventional method.

As a result, Bacillus subtilis KBC1010, Streptomyces natalensis , and Pseudozyma flocculosa were most effective in medium containing 10% by weight jellyfish pulverization. The growth curve was shown, and the higher the concentration was added, the higher the percentage of protein contained in the jellyfish. Ampelomyces quisqualis showed an effective growth curve in medium containing 15% and 20% by weight jellyfish pulverization ( FIGS. 1 and 2 ).

<Example 3> Bacillus subtilis in a medium containing jellyfish pulverization ( Bacillus subtilis ) Chain Formation Analysis of KBC1010

In order to prepare a medium for inhibiting chain formation, the present inventors cultured Bacillus subtilis KBC1010 in a medium containing the jellyfish pulverized product prepared in Example 1 and analyzed whether the chain was formed. Specifically, as a control group, a medium containing 10% by weight of jellyfish ground product adjusted to a moisture content of 67% was prepared as a control group. Thereafter, Bacillus subtilis KBC1010 was cultured in each medium, and the formation of the chain was observed under a microscope.

As a result, the control group began to form chains after 4 hours of cultivation, and after 24 hours, most strains formed chains, but strains cultured in a medium containing jellyfish pulverized did not form more than 70% of chains ( 3 ).

Example 4 Analysis of Natamycin Production of Streptomyces Natalensis

The present inventors examined the content of natamycin produced by Streptomyces natalensis cultured in a medium containing the jellyfish pulverized powder prepared in Example 1. Specifically, a medium containing 10% by weight of jellyfish ground product adjusted to a moisture content of 67% in a nutrient medium was prepared as a control medium, and a nutrient medium without adding jellyfish ground product. Thereafter, each medium was inoculated with Streptomyces natalensis and incubated at 30 ° C. for 4 days to increase the natamycin content in the agar diffusion method (Egorov, NS, et al., 1985. In Antibiotic, a Scientific Approach, English Edition.MIR Publisher, Moscow).

As a result, the content of natamycin in the control group was 0.23 g / L, but the content of natamycin in the strain cultured in the medium containing jellyfish pulverized was 0.34 g / L ( Fig. 4 ).

Example 5 Analysis of Total Lipid Production of Pseudojima Floculosa

The inventors examined the total lipid content (%) produced by Pseudozyma flocculosa cultured in a medium containing the jellyfish pulverized product prepared in Example 1. Specifically, as a control group, a medium containing 10% by weight of jellyfish ground product adjusted to a moisture content of 67% was prepared as a control group. Then, inoculated with Pseudozyma flocculosa in each medium and incubated at 30 ° C. for 4 days to determine the total lipid content (%) in the biomass by the chloroform-methanol method (Folch, et al., J. Biol. Chem. , 226, 497-509, 1957).

As a result, in the control group, the total lipid content was 24.3 g / L, but the total lipid content of the strain cultured in the medium containing jellyfish pulverization was high as 27.9 g / L ( FIG. 5 ).

<Example 6> Ampelomyces quiscusis ( Ampelomyces quisqualis Control Effect of Cucumber Powdery mildew

The present inventors examined the effect of controlling the cucumber powdery mildew of Ampelomyces quisqualis cultured in the medium containing the jellyfish pulverized product prepared in Example 1 above. Specifically, a medium containing 10% by weight of jellyfish pulverized powder adjusted to a moisture content of 67% in a nutrient medium and a nutrient medium not added jellyfish pulverized was prepared. Subsequently, each medium was inoculated with Ampelomyces quisqualis and incubated at 30 ° C. for 4 days to recover spores. Meanwhile, powdery mildew fungus ( Sphaerotheca) isolated from Balsam. ) Was infected with cucumber leaves. Thereafter, the recovered spore supernatant (10 ⁶ spores / ml) was treated with infected cucumber leaves to investigate the powdered powder area ratio after 8 days.

Effect of Ampelomyces quisqualis on Powder Powder Disease Treatment group on cucumber leaf Lesion area ratio (%) Spore supernatant (10 ⁶ spores / ml) incubated in culture medium containing 10% by weight jellyfish crushed 10.2 Spore supernatant cultured and recovered only in nutrient medium (10 ⁶ spores / ml) 12.3 Water 75.6

As a result, the control effect of cucumber powdery mildew was higher than that of Ampelomyces quisqualis cultured in a medium containing jellyfish pulverization than in the control (water treatment) and nutrient medium containing no jellyfish pulverization. The control effect was excellent ( Table 3 ).

<Example 7> Bacillus subtilis ( Bacillus subtilis Extracellular Conditions of Control Effects of KBC1121 on Foot Blight in vitro Test in)

The present inventors have a control effect against Ralstonia solanacearum , a foot blight pathogen of Bacillus subtilis KBC1121 (KCTC 10411BP), cultured in a medium containing the jellyfish pulverized in Example 1 I looked at it. Specifically, as a control group, a medium containing 10% by weight of jellyfish ground product adjusted to a moisture content of 67% was prepared as a control group. Thereafter, Bacillus subtilis KBC1121 was incubated at 50 ° C. for 2 days in each medium. 50 μl of the culture solution was inoculated in Petri dish inoculated with Ralstonia Solanasearum to compare the size of the low-ring ring.

As a result, the size of the ring was 9 mm in the control group, 21 mm in the medium containing jellyfish pulverization. That is, it was confirmed that Bacillus subtilis KBC1121 cultured in a medium containing jellyfish pulverization is excellent in controlling foot blight disease ( FIG. 6 ).

Example 8 Fermentation Odor Analysis of Bacillus Subtilis KBC1121 in a Medium Containing Jellyfish Crushed

The present inventors examined whether the production of fermentation odor for the culture in the medium containing jellyfish crushed in the nutrient medium. Specifically, a medium containing the jellyfish pulverized powder, in which the jellyfish pulverized powder was not added, was used as a control and the moisture content was adjusted to 67% in the nutrient medium as the experimental group. Thereafter, Bacillus subtilis KBC1121 was incubated in each of the medium, and then the culture solution was subjected to a panel test for flavor through an experienced steering technician.

Fermentation of Bacillus subtilis KBC1121 grown in a medium containing jellyfish pulverization Amount of jellyfish grounds in the medium (% by weight) 0 5 10 20 30 40 50 score + ++ ++ +++ +++ +++ +++

+: Normal ++: good +++: very good

As a result, it was confirmed that the fermentation odor of the culture was good in both the medium containing 50% by weight jellyfish pulverization, including the medium containing 20% by weight jellyfish pulverization ( Table 4 ).

Example 9 Formulation Stability Test

The inventors tested the formulation stability to confirm that the nutrient medium containing the jellyfish pulverization of the present invention maintains its stability as Bacillus subtilis KBC1010 culture medium over time. Specifically, after preparing a medium containing 0 to 50% by weight jellyfish pulverized, 5% by weight of polypropylene glycol (PPG) was added as an excipient and bottled and stored at room temperature. Then, after inoculating Bacillus subtilis KBC1010 in the medium, the number of colonies remaining in the medium (cfu / ㎖) according to the storage period of the medium was investigated.

Number of remaining colonies in the medium after inoculation with Bacillus subtilis KBC1010 in the medium Storage period Residual colony number (cfu / ml) in medium according to jellyfish crush content (% by weight) 0 5 10 15 20 30 50 75 0 months 2X10 ⁸ 2X10 ⁸ 4X10 ⁸ 7X10 ⁸ 3X10 ⁸ 2X10 ⁸ 7X10 ⁷ 2X10 ⁷ 1 month 8X10 ⁷ 1 X ^{10 8} 3X10 ⁸ 6X10 ⁸ 2X10 ⁸ 1 X ^{10 8} 6X10 ⁷ 1 X 10 ⁷ 2 months 5X10 ⁷ 8X10 ⁷ 3X10 ⁸ 6X10 ⁸ 1 X ^{10 8} 9X10 ⁷ 4X10 ⁷ 9X10 ⁶ 3 months 4X10 ⁷ 3X10 ⁸ 2X10 ⁸ 5X10 ⁸ 9X10 ⁷ 7X10 ⁷ 2X10 ⁷ 6X10 ⁶ 4 months 2X10 ⁷ 2X10 ⁸ 9X10 ⁷ 4X10 ⁸ 8X10 ⁷ 5X10 ⁷ 1 X 10 ⁷ 5X10 ⁶ 5 months 1 X 10 ⁷ 9X10 ⁷ 4X10 ⁷ 3X10 ⁸ 7X10 ⁷ 3X10 ⁷ 9X10 ⁶ 3X10 ⁶ 6 months 9X10 ⁶ 4X10 ⁷ 3X10 ⁷ 3X10 ⁸ 7X10 ⁷ 1 X 10 ⁷ 7X10 ⁶ 1 X 10 ⁶ 7 months 8X10 ⁶ 3X10 ⁷ 1 X 10 ⁷ 2X10 ⁸ 6X10 ⁷ 9X10 ⁶ 4X10 ⁶ 9X10 ⁵ 8 months 6X10 ⁶ 1 X 10 ⁷ 8X10 ⁶ 1 X ^{10 8} 6X10 ⁷ 7X10 ⁶ 2X10 ⁶ 7X10 ⁵ 9 months 2X10 ⁶ 8X10 ⁶ 5X10 ⁶ 9X10 ⁷ 5X10 ⁷ 6X10 ⁶ 1 X 10 ⁶ 5X10 ⁵ 10 months 9X10 ⁵ 5X10 ⁶ 2X10 ⁶ 8X10 ⁷ 4X10 ⁷ 4X10 ⁶ 9X10 ⁵ 4X10 ⁵ 11 months 7X10 ⁵ 2X10 ⁶ 7X10 ⁵ 6X10 ⁷ 3X10 ⁷ 3X10 ⁶ 7X10 ⁵ 2X10 ⁵ 12 months 6X10 ⁵ 7X10 ⁵ 6X10 ⁵ 5X10 ⁷ 2X10 ⁷ 1 X 10 ⁶ 5X10 ⁵ 1 X 10 ⁵

As a result, up to 30% by weight of the jellyfish pulverized containing the higher the viability and activity of the bacteria compared to the control was maintained ( Table 5 ).

As described above, the medium containing the jellyfish pulverized product of the present invention inhibits the formation of microorganism chains and increases the survival of the strain as well as inhibits the fermentation odor, thereby increasing the activity of the microorganisms, the jellyfish pulverized product of the present invention. When the microbial preparation is prepared by culturing the microorganisms in the medium, the stability and antimicrobial activity of the preparation is increased. Therefore, the medium containing the jellyfish pulverized product of the present invention can be usefully applied in the preparation of microbial preparations.

Claims (17)

A medium for microbial culture, wherein the jellyfish pulverized product is further added. The medium according to claim 1, wherein the jellyfish is selected from the group consisting of remission fly neck, cruciform jellyfish neck, cubic jellyfish neck, myopic jellyfish neck, and balloon jellyfish neck. The medium according to claim 2, wherein the instrument jellyfish neck is a water jellyfish. The medium according to claim 1, wherein the water content of the jellyfish pulverization is 50 to 70%. The medium according to claim 1, wherein the jellyfish pulverized product is included in an amount of 1 to 99% by weight. delete The medium according to claim 5, wherein the jellyfish pulverized product is included in an amount of 5 to 15% by weight. The medium according to claim 1, wherein the medium is for culturing microorganisms. 9. The method of claim 8 wherein the microorganism is Bacillus (Bacillus) genus Lactobacillus bacteria (Lactobacillus) genus Pseudomonas (Pseudomonas) in the alkali jeneseu (Alcaligenes) genus Enterobacter (Enterobacter) genus Streptomyces (Streptomyces), A From the group consisting of the genus Ampelomyces , the genus Pseudozyma , the genus Trichoderma , the genus Aspergillus, the genus Penicillium , and the genus Acremonium . Medium, characterized in that selected. delete The method of claim 9, wherein the microorganisms are Bacillus subtilis , Streptomyces natalensis , Ampelomyces quisqualis and Pseudozyma flocculosa Medium is selected from the group consisting of. The medium of claim 11, wherein the microorganism is Bacillus subtilis . delete (1) washing the jellyfish; (2) pulverizing and dehydrating the washed jellyfish of step 1 to obtain a jellyfish pulverized product; And (3) The method of claim 1, comprising adding the jellyfish pulverized product obtained in step 2 to the medium. A method of inhibiting chain formation of cultured microorganisms by culturing the microorganisms in the medium of claim 1. Method of inhibiting the production of fermentation odor of cultured microorganisms by culturing the microorganisms in the medium of claim 1. A method of increasing the antimicrobial activity of cultured microorganisms by culturing the microorganisms in the medium of claim 1.