KR100407037B1 - Remedy For Obesity Therapy Contained With Green Tea Extractive - Google Patents

Abstract

The present invention relates to an anti-obesity agent containing green tea extract.

The obesity treatment agent containing the green tea extract according to the present invention is added 5 to 20 parts by weight of distilled water to 1 part of dry green tea leaf powder and heated at 60 ° C. to 110 ° C. in hot water for 15 minutes to 1 hour, and the green tea leaf The powder was removed by filtration under reduced pressure, cooled to 4 ° C., and the precipitate was filtered under reduced pressure to obtain a green tea extract. The green tea extract obtained was dried and pulverized, and ethyl ether was added and stirred to remove the ether layer. It is characterized by containing a polyphenol produced by the process comprising a main component and free amino acids, free sugars, caffeine and inorganic ions.

The obesity treatment agent containing the green tea extract according to the present invention inhibits the process of decomposing starch into disaccharides, thereby reducing caloric absorption and treating obesity.

Description

Remedy For Obesity Therapy Contained With Green Tea Extractive}

The present invention relates to an anti-obesity agent containing green tea extract.

It has been known in the folklore that green tea as a tea has an effect on diabetes and obesity, but specific and scientific studies on the effect of tea extracts on obesity have not been reported.

In general, when making tea as a favorite food, green tea was brewed in water at a temperature of 60 to 80 ° C. for 3 to 5 minutes, and drinking. However, polyphenol, which is the main ingredient of the preparation, requires more time to be released and extracts components useful for the prevention and treatment of obesity contained in green tea by separating and purifying physiologically active substances.

Agents used in the treatment of diabetes mellitus include agents that increase the use of sugar in the body and agents that lower the absorption of sugar. Starch is mainly broken down into disaccharides or trisaccharides by amylase of the pancreas, and they are broken down into glucose, a monosaccharide by alphaglycosidase in the small intestine.

The cause of obesity is caused by the imbalance between calorie absorption and utilization of the living body. If the amount of calorie absorption is larger than the amount used, obesity is caused by the accumulation of body fat. Therefore, to treat obesity, exercise therapy to increase calorie use and diet to reduce calorie intake are implemented.

Reducing the amount of calories is a good way to reduce the absorption of calories, but because it is difficult to implement the adjuvant therapy digestive enzyme inhibitors can be used as an obesity treatment. Xenical et al. Currently used as a therapeutic agent for obesity is a lipase inhibitor which is a lipoprotein. The dietary characteristics of Korean people are the most sugar (50-70%) of calories (sugar, lipids, proteins), and the intake of starch in sugar is particularly high because rice is the staple food. Therefore, in people with high intake of carbohydrates, lipase inhibitors are not suitable for treating obesity. Digestion of starch is broken down into disaccharides or trisaccharides by the digestive enzyme amylase, and they are broken down into glucose, a monosaccharide, by alphaglycosidase in the small intestine. Currently, agents that inhibit alphaglycosidase, a disaccharide degrading enzyme, have been used for the treatment of diabetes and obesity-type diabetes in order to reduce the digestive absorption of sugar and to suppress the increase in blood glucose. When alphaglycosidase is inhibited, disaccharides, which are degradation products of starch, may be increased, which may cause diarrhea due to an increase in intestinal osmotic pressure, and may cause side effects such as increased gas production as disaccharides are degraded by intestinal bacteria. Inhibiting the breakdown of starch into disaccharides can reduce these side effects, and there are no examples of commercially available agents with a mechanism of action that inhibits the breakdown of starch into disaccharides as therapeutic agents for obesity.

The present invention is to reduce the calorie absorption by inhibiting the action of digestive enzymes to treat obesity that can cause various diseases such as diabetes, hypertension, starch digestion, absorption rate by extracting the agent to inhibit starch digestive enzymes in green tea The present invention seeks to provide an anti-obesity agent that can reduce body weight.

That is, according to the present invention, by adding 5 to 20 parts by weight of distilled water to 1 part by weight of dried green tea leaf powder and heating for 15 minutes to 1 hour in hot water of 60 ℃ to 110 ℃, the green tea leaf powder by vacuum filtration Removing and cooling to 4 ° C. and filtering the precipitate under reduced pressure to obtain a green tea extract, and drying and pulverizing the green tea extract obtained, adding ethyl ether and stirring to remove the ether layer. Provided is a therapeutic agent for obesity, characterized by containing the produced polyphenol as a main component and containing free amino acids, free sugars, caffeine and inorganic ions.

When drinking green tea as a favorite product, it is generally used for 3-5 minutes in water of 60-80 ° C., but the polyphenol, which is an active ingredient of the extract of the present invention, requires more time and intense conditions. Therefore, in the present invention, dried green tea leaf powder or raw green tea leaf is boiled for 15 minutes or more in hot water of 60-110 ° C., cooled to 4 ° C. to filter and remove the precipitate, and then dried and pulverized, an organic solvent. Extraction again to remove highly soluble substances in organic solvents.

The prepared extract can be used as it is or in the form of powder by dry grinding for convenience of formulation and taking.

The green tea extract prepared according to the method of the present invention is a preparation having a function of inhibiting the decomposition of starch into maltose, and reduces the digestion and absorption of diet in people whose grains contain a lot of starch (eg, rice). Since there is an effect of reducing body fat, according to the present invention can be provided an anti-obesity agent containing the extract.

Green tea extract according to the method of the present invention may be made in a conventional formulation and administered orally or parenterally. The dosage form is not particularly limited, but is more preferably administered orally in the form of a liquid, powder, capsule, tablet, syrup and the like.

Since the green tea extract preparation according to the present invention is extracted from natural foods, it is not toxic to the human body, and the dosage may be appropriately adjusted according to the purpose of administration and the condition, age, and weight of the patient, but in the case of the dry extract, it is administered per adult The amount may suitably be selected from several hundred mg to several g, and in the case of extracts, from several ml to several hundred ml per adult.

Example

The present invention will be described in more detail with reference to the following examples. However, the present invention is not limited to these examples.

Example 1: Preparation of Green Tea Extract

10 liters of distilled water was added to 1 Kg of hot air dried green tea leaf powder, and heated under reflux for 30 minutes. The green tea leaf powder was removed by filtration under reduced pressure, and left at 4 ° C. for 1 hour, followed by filtration under reduced pressure to obtain 8.5 liters of the extract. The obtained extract (8.5 L) was spray-dried and pulverized to obtain 230 g of green tea extract powder. Ethyl ether (500 ml) was added thereto and stirred for 30 minutes to remove a substance soluble in ethyl ether.

The obtained green tea extract (210 g) was dried, and the dried extract was ground to obtain 200 g of green tea extract (A).

About the green tea extract (A) of the present invention obtained, the components were analyzed in the following manner.

Component Analysis

Free amino acid: measured at 570 nm by color development using ninhydrin

Free sugar: HPLC method

Caffeine: HPLC method

Inorganic ion: Molecular absorbance photometer

Polyphenols: Spectrophotometric redox assay by Prussian blue

The result of component analysis of the obtained green tea extract is as follows.

ingredient content (%) amino acid 11 Free sugar (sugar syrup, fructose, glucose) 12 Polyphenols 51 Caffeine 4 Minerals (potassium, calcium, magnesium) 6 Etc 16

Example 2: Amylase Inhibitory Effect of Green Tea Extract

A. Starch Load Experiment

Experimental purpose: Starch is decomposed into maltose by the action of amylase, and the maltose is decomposed into glucose by alphaglycosidase and absorbed. Therefore, the effect of green tea extract on the digestive absorption of starch by starch was examined by measuring blood glucose .

Experimental Methods: Sprague-Dowley male rats weighing 220-250 g were tested. After fasting the experimental animals for 12 hours, each group was divided into 5 control groups, starch administration group, starch and green tea extract administration group, and the mixture of tap water (3 ml) in the control group and 1 g of starch in the starch administration group was dissolved in tap water. (3 ml), the co-administration group was administered a mixture (3 ml) of 1 g of starch and green tea extract (A) (60 mg) prepared in Example 1 dissolved in tap water. After administration of the sample and 40 minutes after the administration of the sample, the tail was dissected and blood was collected to measure blood glucose levels.

The experimental results are shown in Table 1 below.

Experimental group Blood sugar (mg / 100ml) Immediately before administration 40 minutes after administration Control 71.5 ± 5.3 72.2 ± 6.7 Starch administration group 71.1 ± 7.3 120.5 ± 15.3 Starch + green tea extract group 70.5 ± 4.2 85.7 ± 7.7

(Value = mean value ± standard deviation)

In the starch group, the blood glucose level was 71.1 ± 7.3 mg / 100ml before starch administration and 120.5 ± 15.3 after 40 minutes of starch administration. Blood glucose levels of starch and green tea extract groups were increased to 70.5 ± 4.2 mg / 100ml before starch + green tea administration and 85.7 ± 7.7 mg / ml after 40 minutes of administration. The blood glucose level of the control group administered only with tap water showed little change in blood glucose levels before and after 40 minutes. In the starch + green tea extract group, the blood sugar level was 34 mg / 100ml lower than that of the starch group, and the green tea extract administration significantly inhibited the increase in blood glucose levels by starch. These experimental results show that green tea extract inhibits the digestion and absorption of starch and thus increases blood glucose levels.

B. Sugar load test

Experimental purpose: Starch is decomposed into maltose by the action of amylase, and maltose is decomposed into glucose by alphaglycosidase and absorbed. Therefore, blood glucose was measured to examine the effect of green tea extract on digestive absorption of disaccharide by administering maltose. It was.

Experimental Methods: Sprague-Dowley male rats weighing 220-250 g were tested. After fasting the experimental animals for 12 hours, each group was divided into 5 controls, maltose administration group, maltose and green tea extract simultaneous administration group, and mixed with tap water (3 ml) in the control group and 1 g of maltose in the maltose administration group in tap water. (3 ml), the co-administration group was administered with a mixture (3 ml) of 1 g of malt and green tea extract (A) prepared in Example 1 (60 mg) dissolved in tap water. Before the administration of the sample and 40 minutes after the administration of the sample, the tail was dissected and blood was collected to measure blood glucose levels.

The experimental results are shown in Table 2 below.

Experimental group Blood sugar (mg / 100ml) Immediately before administration 40 minutes after administration Control 72.3 ± 4.5 72.2 ± 5.4 Fucked 70.2 ± 4.3 117.9 ± 13.5 Malt sugar + green tea extract group 71.2 ± 5.4 121.9 ± 10.5

(Value = mean value ± standard deviation)

The blood sugar of the maltose-treated group increased to 70.2 ± 4.3 mg / 100ml before the maltose administration and to 117.9 ± 13.5 after 40 minutes of maltose administration. The blood sugar level of maltose and green tea extract group was 71.2 ± 5.4 mg / 100ml before maltose + green tea administration and 121.9 ± 10.5 mg / ml after 40 minutes of administration. The blood glucose level of the control group administered only with tap water showed little change in blood glucose levels before and after 40 minutes. There was almost no difference in blood glucose increase between maltose + green tea extract group and maltose-treated group. These results show that green tea extract has no effect on the digestion and absorption of maltose.

C. Glucose Load Test

OBJECTIVES: Starch was broken down into maltose by amylase action and maltose was broken down into glucose by alphaglycosidase. So, glucose was administered to investigate the effect of green tea extract on the absorption of monosaccharides. .

Experimental Methods: Sprague-Dowley male rats weighing 220-250 g were tested. After fasting the experimental animals for 12 hours, each group was divided into 5 control groups, glucose administration group, glucose and green tea extract simultaneous administration group, and a mixture of tap water (3 ml) in the control group and 1 g of glucose in the glucose administration group was dissolved in tap water. (3 ml), the co-administration group was administered a mixture (3 ml) in which 1g of glucose and green tea extract (A) (60 mg) prepared in Example 1 were dissolved. After administration of the sample and 40 minutes after the administration of the sample, the tail was dissected and blood was collected to measure blood glucose levels.

The experimental results are shown in Table 3 below.

Experimental group Blood sugar (mg / 100ml) Immediately before administration 40 minutes after administration Control 72.1 ± 4.7 71.7 ± 3.7 Glucose administration group 71.8 ± 6.1 121.5 ± 12.1 Glucose + Green Tea Extract Administration Group 72.5 ± 5.3 126.6 ± 17.1

(Value = mean value ± standard deviation)

The blood glucose level of the glucose group was 71.8 ± 6.1 mg / 100ml before glucose and 121.5 ± 12.1 after 40 minutes of glucose administration. The blood glucose level of glucose and green tea extract group was 72.5 ± 5.3 mg / 100ml before glucose + green tea administration and 126.6 ± 17.1 mg / ml after 40 minutes of administration. In the control group that received only tap water, there was little change in blood glucose before and 40 minutes after the administration of the sample. There was little difference in blood glucose increase between glucose + green tea extract group and glucose group. These experimental results show that green tea extract has no effect on glucose absorption.

As shown in Example 2, starch is decomposed into maltose by the action of amylase, and maltose is decomposed into glucose by alphaglycosidase and absorbed to increase blood sugar, by administering starch, maltose, and glucose. The effect of green tea extract on the digestion and absorption of starch was examined by measuring blood glucose. Green tea extract inhibited the increase of blood glucose level by starch administration, but did not inhibit the increase of blood glucose level by the administration of maltose or glucose. Suggests that it contains substances that inhibit the digestive process in which starch is broken down into maltose.

Example 3: Neutral Lipid Reduction and Weight Loss Effect of Green Tea Extract

OBJECTIVES: The effects of green tea extracts containing substances that inhibit the process of starch decomposition into maltose were investigated in obese patients by measuring their weight and blood triglyceride mass.

Experimental Method: The dried green tea extract (A) obtained in Example 1 was administered to each volunteer (BMI 30 or more) determined to be obese for 1 week in a meal (3 times a day) for 4 weeks. Body weights were measured weekly and blood was collected at two week intervals to determine the mass of blood glucose and serum neutral fat.

The experimental results are shown in Table 4 below.

start 1 week 2 weeks 3 weeks 4 Weeks Weight change (kg) 0 -0.5 -1.5 -2.0 -2.5 Blood glucose change (mg / 100 ml) 0 -2 -3 Neutral lipid change (mg / 100 ml) 0 -35 -45

(Numeric value)

Body weight index (BMI) 30 or more volunteers with dry green tea extract (A) obtained in Example 1 was administered for 4 weeks at a meal intervals (3 times a day) for 4 weeks, and the weight was measured before meals. Body weights were 500 g after one week, 1500 g after two weeks, 2000 g after three weeks, and 2500 g after four weeks, respectively. Blood glucose and blood triglycerides were measured by taking blood before meal at intervals of two weeks after the start of the experiment. Blood glucose levels did not change, and blood neutral lipids were 35 mg / 100ml after two weeks, and after four weeks. 45 mg / 100 ml were each reduced. These results suggest that green tea extract can significantly reduce blood neutral lipids and body weight in obese patients.

Example 4 Liver and Kidney Toxicity Test of Green Tea Extract

Purpose: To evaluate the acute toxicity of green tea extract by administering green tea extract to rats.

Experimental Methods: Sprague-Dowley male rats weighing 220-250 g were tested. After fasting the experimental animals for 12 hours, each group was divided into 10 control groups, green tea extract administration group, tap water (2 ml) in the control group, green tea extract (A) obtained in Example 1 in the green tea extract administration group (100 mg) ) Was dissolved in tap water (2 ml) and administered orally. After 1 day, rats were sacrificed and blood was collected from the abdominal artery, and blood glucose, BUN, sGOT, sGPT, alkaline phosphatase, and creatine were measured using blood biochemical analyzers, respectively.

The experimental results are shown in Table 5 below.

Control Green tea extract administration group Glucose (mg / 100ml) 98 ± 11 95 ± 8 BUN (mg / 100ml) 21 ± 7 19 ± 6 Creatine (mg / 100ml) 1.2 ± 0.1 1.1 ± 0.1 sGOT (U / L) 58 ± 11 59 ± 8 sGPT (U / L) 65 ± 9 67 ± 18 Alkaline phosphatase (U / L) 125 ± 32 126 ± 45

(Value = mean value ± standard deviation)

The experimental animals were categorized into the control group and the green tea extract administration group which were fasted for 12 hours. The control group was orally administered with 2 ml of tap water, and the green tea extract administration group with 2 ml of a solution of 200 mg of green tea extract (A) obtained in Example 1 After 1 day, blood was collected and blood glucose, BUN, sGOT, sGPT, alkaline phosphatase and creatine were measured. SGOT, sGPT and alkaline phosphatase used as markers of liver damage did not differ between the control and green tea extract groups, and the amount of BUN and creatine used as markers of kidney damage did not differ between the control and green tea extract groups. These results indicate that green tea extract does not show acute toxicity to rat liver or kidney.

As shown from the experimental results of the above embodiment, the anti-obesity agent containing the green tea extract according to the present invention inhibits starch from being degraded into maltose, thereby inhibiting sugar digestion and absorption. In addition, by suppressing digestive absorption of starch, calorie absorption can be lowered and weight gain can be suppressed.

Those skilled in the art will appreciate that various changes and modifications can be made without departing from the technical spirit of the present invention. Therefore, the technical scope of the present invention should not be limited to the contents described in the detailed description of the specification but should be defined by the claims.

Claims (1)

5 to 20 parts by weight of distilled water was added to 1 part of dry green tea leaf powder and heated in hot water at 60 ° C. to 110 ° C. for 15 minutes to 1 hour, and the green tea leaf powder was removed by filtration under reduced pressure and cooled to 4 ° C. The precipitate was filtered under reduced pressure to obtain a green tea extract, and the obtained green tea extract was dried and pulverized, and then ethyl ether was added and stirred to remove the soluble substance in ethyl ether. An anti-obesity agent containing phenols, free amino acids, free sugars, caffeine and inorganic ions.