KR100381371B1 - A feedingstuff including microorganism and manufacture method of feedingstuff - Google Patents

Abstract

The present invention relates to a livestock manure treatment method, a livestock feed additive comprising a microorganism used therein, and a method for producing the livestock feed additive.

The object of the present invention is not to discharge the livestock, but to use as a recycling resource such as fermented feed, as well as to increase the efficiency of treatment of livestock manure during treatment of livestock manure with excellent water pollution source, especially TN treatment, significantly reducing odor and pathogenic bacteria It provides a livestock feed additive comprising a microorganism to make and a method for preparing the same.

Livestock feed manufacturing method of the present invention for achieving the above object, the step of separating the manure from the livestock breeding livestock with animal feed additives containing fermentation microorganisms together with livestock feed; Weighing and mixing the separated powders and forages, respectively; Fermentation and drying by adding a fermentation agent to the metered powder and forage; Sorting the fermented dried mixture by crushing and particle size; Aging the selected mixture; Fermenting the separated urine and small amounts of unseparated flour; Aeration of the fermented manure and evaporating the generated manure foam; Precipitating manure not evaporated in the evaporation step; And discharging the supernatant not precipitated in the precipitation step.

The fermentation microorganisms include naphtha bacteria, photosynthetic bacteria, lactic acid bacteria, and yeast.

In addition, animal feed additives include naphtha bacteria, photosynthetic bacteria, lactic acid bacteria, and yeasts.

In addition, the method of manufacturing livestock feed additives, a) Bacillus subtilis CP220 KCTC 8831P, photosynthetic bacteria (Rhodospirillum rubrum), Lactohacillus acidophilus, and yeast (Saccharomyces cerevisiae) four microorganisms together Culturing for about 2 to 3 days in a culture medium containing sugar, soy flour, and skim steel or ethanol: b) water of the mixture by adding ocher, skim steel, activated carbon, and zeolite to the mixed cultures after the incubation. To 40 to 45%; And c) a fermentation microorganism comprising fermenting the mixture at a temperature of about 40 ° C. for 6-7 days.

Description

A feedstuff including microorganism and manufacture method of feedingstuff

The present invention relates to a livestock manure treatment method, a livestock feed additive comprising a microorganism used therein, and a method for producing the livestock feed additive, and particularly, a solid-liquid separator for livestock manure fed with feed additives containing anaerobic microorganisms. After separation into raw powder and urine, the stock is mixed with fertilizer by crab, fermented for 3-4 hours in fermentation dryer, aged for 6-12 hours through grinding and screening, and made into fermented feed. The feedstock of the present invention relates to a livestock manure treatment method which is fermented, aerated, decomposed and evaporated into the atmosphere, a livestock feed additive containing microorganisms used therein, and a method of producing the livestock feed additive.

Today, manure produced when raising livestock such as cattle, pigs, and horses causes serious environmental pollution, but since undissolved manure is discharged, water pollution is severe, and ammonia from livestock is no less than this water pollution. There are also serious odors caused by gas and 2.3-benzophenone and pathogenic bacteria that can cause disease in humans and livestock.

In other words, the environmental pollution problem is harmed not only to humans but also to livestock.

Since the renal disease of the livestock and the deterioration of the meat caused by the stress due to flies, for example, it has been an important technical task to reduce the environmental problems by treating these manure effectively.

The treatment of livestock manure is a method of treating manure by mixing or by separating the manure to separate the manure and urine separately.

First, the treatment of manure separately is as follows.

First, urine and urine are separated, and the separated urine is processed through activated sludge method, water trickling method, oxidation paper method, and trench method.

Among these methods, the Sludge Process requires a lot of technical skills for maintenance, requires the treatment of surplus sludge, expensive facility and maintenance costs, and the Sprinkler Award method has a slightly poor purification effect and is not suitable for large-scale management. It is easy but has a disadvantage of requiring a lot of paper, and the Transit Law requires a small amount of expenses, but the renewal of the enlarged area is required every two to three years.

Most of the above methods are commonly treated with urine as a primary treatment, microbial treatment as a secondary treatment, inorganic decomposed organic matter, nitrogen and phosphorus, etc. which have not been removed by primary and secondary treatments. Treat by removing nutrients.

However, even if the discharge of the urine of the livestock by primary, secondary and tertiary treatment, it is difficult to completely purify, so that water pollution can not be completely prevented, and there is a severe problem of the odor of the livestock due to the manure of the livestock.

In addition, the separated powder can be used as raw powder, composting, thermal drying or drying in a plastic house, and incineration. However, when using raw powder, the processing cost is low, but it is inconvenient to handle and use. Seasoning problem, composting has a wide range of use and a relatively low treatment cost, but it needs to be adjusted to moisture suitable for composting (particularly for pigs, mixed with sawdust or rice husk as a moisture control agent and fermented for 3 ~ 6 months. Drying in plastic houses is less expensive and easier to handle, but requires more paper than other treatments, and incineration is problematic in terms of recycling resources.

In addition, as a method of treating manure together, there is a method of treating fermented manure obtained by adding fermented purified product fermented by aerobic bacteria to a feed through fermentation, aeration and precipitation, but it is not digested completely. Many foreign materials such as irradiated food, hair, etc. are present, which lowers the efficiency of fermentation and aeration, clogged feed pipes, and causes a malfunction of treatment equipment such as aeration pumps.

In addition, there is a problem that the defoaming machine that decomposes the bubbles generated by the condensation treatment into the air of the conventional condensation treatment apparatus has a low performance, the temperature is 35-65 ℃ for the removal of low-temperature pathogenic bacteria and the growth of fines It should be maintained, but there is a problem that can not be maintained in the winter, and like the other conventional methods do not solve the problems caused by odor or pathogenic bacteria.

The present invention does not discharge the livestock, utilizes it as a recycling resource such as fermented feed, as well as to increase the efficiency of treatment of livestock manure during treatment of livestock manure with excellent water pollution, especially TN treatment, and significantly reduce the odor and pathogenic bacteria Provides livestock feed additives.

1 is a plan view showing the overall treatment of the livestock manure,

2a shows a circular sorter,

2b is a square separator,

3 is a cross-sectional view of the fermentation dryer in the condensate treatment device,

4 is a cross-sectional view taken along the line A-A of Figure 1, a fermentation device and aeration device of the urine treatment device,

5 is a plan view showing a small aerator of the aeration device,

6 is a sectional view taken along the line B-B of Figure 1 showing the sedimentation tank and the storage tank in the urine storage device.

7 is a process chart showing a method of treating livestock manure.

<Description of Symbols for Main Parts of Drawings>

1: slurry tank 10: solid-liquid separator

20: weighing hopper 30: fermentation dryer

40: crusher 50: sorter

60: aged hopper 70: fermenter

80: aeration tank 90: sedimentation tank

In order to achieve the above object, the present invention comprises the steps of separating the manure from raising livestock with livestock feed additives containing fermentation microorganisms together with livestock feed into manure and urine; Weighing and mixing the separated powders and forages, respectively; Fermentation and drying by adding a fermentation agent to the metered powder and forage; Sorting the fermented dried mixture by crushing and particle size; Aging the selected mixture; Fermenting the separated urine and small amounts of unseparated flour; Aeration of the fermented manure and evaporating the generated manure foam; Precipitating manure not evaporated in the evaporation step; And discharging the supernatant not precipitated in the precipitation step.

The fermentation microorganism, Bacillus subtilis, photosynthetic bacteria (Rhodospiril-lum rubrum), lactic acid bacteria (Lactohacillus acidophilus), and yeast (Saccharomyces cerevi -siae).

Meanwhile, animal feed additives include Bacillus subtilis, photosynthetic bacteria (Rhodo-spirillum rubrum), lactobacillus (Lactohacillus acidophilus), and yeast (Saccharomyces cerevisiae).

In addition, the method of producing livestock feed additives, a) Bacillus subtilis CP220 KCTC 8831P, each of the cultured bacteria (Rhodospirillum rubrum), Lactohacillus acidophilus, and four kinds of yeast (Saccharomyces cerevisiae) together with the microorganism of 40 ℃ Culturing for about 2 to 3 days in a culture medium containing sugar, soy flour, and skim steel or marinated liquor: b) adding the ocher, skim steel, activated carbon, zeolite to the mixed culture, the water of the mixture 40 to 45%; And c) a fermentation microorganism comprising fermenting the mixture at a temperature of about 40 ° C. for 6-7 days.

Looking at the function of each microorganism is as follows.

Naphtha is a anaerobic bacterium that promotes the suppression of intestinal harmful bacteria, the growth of intestinal effective bacteria, the promotion of other microorganisms, the production of digestive enzymes capable of digesting wood and proteins, and the absorption of calcium. Specifically, the Bacillus bacterium produces Dipicolinic acid to inhibit pathogenic harmful bacteria such as Salmonella, Staphylococcus aureus, typhoid bacilli, E, and coli 0-157. In general, bacteria belonging to Bacillus family including Bacillus bacteria have been reported to produce several antibiotics such as bacitricin, polymixin and urethin. Naphtha bacteria also survives not only in feed products but also in heat or gastric acid, and enters the intestinal tract with a strong vitality due to sporulation. Especially, residual fibrin in the large intestine and the fructose produced by the naphtha bacterium combine to effectively intestinal effective bacteria. Helps multiply. In addition to the proteases, amylases, and lipases that break down nutrients, the bacterium produces a number of key enzymes, including cellulase and ureaso, peroxidase, catalase, and pectinase, which are essential for woody digestion. To be Bacillus Bacillus produces a group of vitamin B and various trace bioactive substances to promote the growth of other microorganisms blended. In addition, it produces vitamin K2, which is necessary as a mediator when combining calcium and r-glutamic acid, which are essential factors for bone formation, to help bone formation, and pluglutamic acid is one of the viscous substances produced by Bacillus pneumoniae. Promote Naphtha bacteria have a faster growth rate than other species. Naphtha bacteria inhibit the intestinal harmful bacteria in the body of animals to reduce the odor by inhibiting the generation of ammonia nitrogen and 2,3 benzophenol due to decay bacteria, and are discharged together with urine to reduce BOD by decomposing organic matter during manure treatment.

Next, when looking at photosynthetic bacteria, as anaerobic bacteria, they play an important role in the decomposition of organic matter and the decomposition of nitrogen oxides during the treatment of manure manure, thereby enhancing the efficiency of manure manure and removing the odor generated from the manure manure. In addition, yeast, naphtha bacteria, lactic acid bacteria to help the growth of mixed cultures with these microorganisms can maximize the culture efficiency and their function. In addition, photosynthetic bacteria contain a large amount of essential amino acids, nucleic acids, vitamins, and bioactive substances in the cells, which is very useful for feed additives. Photosynthetic bacteria are capable of denitrification, proliferate even with high salinity, grow well with or without light and oxygen, and are effective for the treatment of manure manure due to their ability to degrade cyanide.

Lactobacillus is an anaerobic bacterium, which forms an effective bacterium in the intestine to produce trace amounts of bioactive substances, and thus has various probiotics effects that inhibit harmful bacteria such as rot bacteria from being eaten in the intestine. In addition, lactic acid bacteria are useful for feed additives because they help digestion of feed ingested, increase nutrient absorption efficiency, improve livestock growth rate, prevent bacterial and viral diarrhea, and prevent acute sepsis by using various kinds of lactic acid bacteria.

Yeast is degraded by stomach acid in the stomach of livestock, and decomposition products contain various bioactive substances such as carbohydrates, fats, proteins and nucleic acids. Therefore, the livestock feed additive including the microorganism according to the present invention has a function as a general feed in addition to the use of manure treatment.

Livestock feed additives containing the microorganisms are prepared through three stages of culture. After incubating each microorganism in the primary culture, each of the primary cultured microorganisms is cultivated in large quantities, and undergoes a secondary culture step to obtain good activity in the tertiary culture. It creates a multiplying environment for microorganisms and contributes to the generation of animal favorite odors.

For example, ocher suppresses harmful and unnecessary bacteria and removes odors that livestock dislike. The effect of the material added during the third culture can further increase the degree of effect by appropriately mixing the ratio of the materials.

Hereinafter, the axial manure treatment apparatus used in the present invention will be described with reference to FIGS. 1 to 6.

The livestock manure treatment device according to the present invention is classified into a livestock waste treatment device and a urine treatment device because the manure from the livestock house is stored in the slurry tank 1 or immediately after being separated into manure and urine.

The device for separating the nutrient manure stored in the slurry tank 1 by the pump (not shown) into the nutrient powder and the urine urine is a solid-liquid separator 10.

As shown in Figure 2, the solid-liquid separator 10, a circular separator (FIG. 2A) and a square separator (FIG. 2B) are used, and inlet 11 for inputting axial manure on top of the solid-liquid separator 10 in any form. Is provided, the lower portion is provided with a discharge port 12 for discharging the condensed manure separated into ureum and urine, and the inside of the solid-liquid separator 10 is formed with a multi-stage passage 13, a mesh (between each passage) A screen 14 of different mechs is supported by the clamp, while the solid-liquid separator 10 is supported by a support 15 on the ground or on top of the manure tank.

A spring 16 is installed between the solid-liquid separator 10 and the support 15, and an electric motor 17 fixed on the support 15 is connected to the lower portion of the solid-liquid separator 10 by a bracket 18. It is designed to transmit the vibration force evenly.

The shaft portion of the shaft manure discharged to the upper part of the three discharge ports 12 of the solid-liquid separator 10 is processed by the following shaft treatment apparatus.

As shown in Figure 1, the axial fraction separated from the solid-liquid separator 10 is transferred to the conveying conveyor 19 is introduced into the metering hopper (20). The weighing hopper 20 used in the present invention may be used as long as it is a device capable of discharging a predetermined amount of constituents by a set amount with a weight sensor attached to the lower portion thereof, and adds a separate hopper as much as the amount of irradiated compound to be mixed. It can also be installed and used.

Shaft and other mixtures measured in the weighing hopper 20 is transferred to the fermentation dryer 30 by a transfer conveyor (29).

As shown in FIG. 3, the fermentation dryer 30 has a burner 33 disposed below the semi-cylindrical fermentation chamber 32 made of a stainless steel double wall 31, and a mixture at the top of the fermentation chamber 32. An inlet 34 is formed, and the oil 35 flows into the double wall 31, and when the fermentation chamber 32 is heated by the burner 33, the heated oil 35 convex inside the double wall 31. The fermentation chamber 32 is heated evenly.

In the center of the fermentation chamber 32, a plurality of cross stirrers 36 are mounted on the rotation shaft 36a, and a lower feed screw 37 is mounted on the lower portion of the fermentation chamber 32, and the rotation shaft ( 36a) and the conveying screw 37 are driven in the forward or reverse direction by separate drive motors 38.

A discharge port 39 is formed in the transport screw protection tube protruding from the fermentation chamber 32 to discharge the fermentation-dried mixture.

Since the mixture fermented and dried in the fermentation dryer 30 is transferred to the crusher 40 by a transfer conveyor 41, the crusher 40 used in the present invention is a device for grinding the fermented mixture into particles of a predetermined size. Any device can be used as long as it is a shredder capable of cutting the mixture, including using a pair of rotating cutting edges.

The mixture pulverized in the crusher 40 is transferred to the separator 50 by the conveying conveyor 49. The separator 50 used in the present invention used the same structure as the vibrating solid-liquid separator 10. The difference is that the mesh of the screen is larger than that used in the solid-liquid separator 10.

The mixture selected by particle size in the separator 50 is transferred to the aging hopper 60 by a transfer conveyor 59, the aging hopper 60 used in the present invention used a aging hopper commonly used.

Accordingly, the aging hopper 60 is equipped with an air nozzle for blowing air, and an impeller driven by a motor is mounted in the hopper so that the mixture being aged is pulverized with stirring.

On the other hand, the urine and the small amount of the urine in the manure discharged to the lower of the three discharge port 12 of the solid-liquid separator 10 is processed by the urine treatment device.

That is, the urine separated from the solid-liquid separator 10 is introduced into the fermentation tank 70 through the transfer pipe 71 as shown in Figure 4, the fermentation tank 70 stores the sedimentary manure separated from the solid-liquid separator 10 It is made of a concrete reservoir 72 so that it can be fermented, and the reservoir 72 is installed with an underwater pump 73 to pump urine in the reservoir to be sprayed into the reservoir through the water spray nozzle 74.

At this time, sprinkling so that water can also be sprayed on the urine that is sprayed.

On the other hand, the aeration tank 80 for aeration while storing the manure manure fermented in the fermentation tank 70 is in communication with the communication hole 76 to the fermentation tank 70.

In addition, the foam of the remaining manure that is not evaporated is supplied to the sedimentation tank 90 which separates the supernatant and the sediment through the conduit 85, and the sedimentation tank 90 is the secondary and tertiary sedimentation tanks 92, It is preferable to add 93).

In addition, through-holes 94 are formed in the barrier separating the primary sedimentation tank 91 and the secondary sedimentation tank 92, and the barrier 95 separating the secondary sedimentation tank 92 and the tertiary sedimentation tank 93 from each other. Is formed lower than the outer wall of the settling tank (90).

The discharged water from the settling tank 90 is collected in the storage tank 96, and an underwater pump 97 is installed at the bottom of the storage tank 96 to supply the discharged water to the barn through the supply pipe 98.

Effluents transferred to the barn are introduced into slurry tanks or recycled to the barn wash water or biofertilizer.

Hereinafter, a treatment method of livestock manure according to the present invention will be described with reference to the treatment process diagram of FIG. 7.

The present invention can be pre-fermented while temporarily storing the manure from raising livestock using a feed additive containing microorganisms together with feed in a slurry tank (1).

At this time, the slurry tank maintains a constant water level with the discharge water transferred from the storage tank so that the nutrient feed is naturally transferred to the solid-liquid separator 10 through the transfer pipe.

In the solid-liquid separator 10, manure manure is separated into manure and urine, and undigested soybean meal, corn, and other forages are also separated like manure.

The separated livestock is mixed with the fertilizer by metering, fermented in the fermentation dryer, pulverized and screened, then aged. This process is as follows.

First, the axial fraction separated by the weighing process is introduced into the weighing hopper 20 and discharged by a predetermined amount, and is discharged by an appropriate amount by a weight sensor at the bottom of the hopper.

At this time, bran, rice bran, ocher, etc. are sequentially metered into the measuring hopper and discharged, and the axial fraction and bran, rice bran, ocher, etc. discharged are mixed while being transferred to the fermentation dryer (30).

The loess mixed with the livestock can be replaced with fishmeal, beer dregs, fruit trash, etc., and it is not necessarily loess, and can be variously applied according to the type of feed.

In the fermentation process, weighed shaft meal, bran, rice bran, ocher, etc. are added to the fermentation dryer (30) together with a small amount of fermentation agent. It is kept at 40%.

And the grinding process is a process of crushing the fermented and dried mixture into a crusher 40 to a particle of a certain size, the sorting process is to separate the crushed mixture particles by the electric separator 50 to separate the foreign matter and mixture by particle size It is a process.

The aging process is a process in which the selected mixture is stirred in the aging hopper 60 and stirred for about 2 days while blowing air supplied through the air nozzles to cultivate the culture of aerobic microorganisms so that the fermentation is completed.

Through this aging process, the livestock is finally made into animal feed.

In this process, the process of sterilization, separate sorting to separate the foreign matter contained in the livestock, and the packaging process to package the feed prepared after the aging process may be added. It is not essential to the present invention.

The urine separated from the solid-liquid separator 10 and a small amount of undivided nutrients are transferred to the fermentation apparatus. That is, the manure is continuously pumped from the fermentation tank 70 corresponding to the fermentation apparatus to the submersible pump 73 to spray the manure at the bottom of the tank through the nozzle 74 installed at the top.

At this time, a certain amount of water is also sprayed together with the manure to make water 30 to 50 parts by weight to 60 parts by weight of manure.

Maintaining the fermentation tank at the above conditions maintains the proper temperature (37-65 ° C.) for the fermentation microorganisms to act, promotes the activity of the microorganisms, can remove the low-temperature pathogenic bacteria, and due to the fermentation of the pathogenic bacteria It can prevent ammonia odor, and when fermented liquid fertilizer is sprayed around it, flies and other bacteria are suppressed.

As a result of this fermentation process, the manure is evaporated, and the manure is fermented while the oxygen supply is increased, thereby promoting the activation of the added microorganisms.

The manure fermented in the fermentation tank is transferred to the aeration tank 80, and introduced into the aeration tank 80 through the air inlet pipe 82 by the air blower pump 83 to generate vortices in the aeration tank.

As a result, organic matter is decomposed into carbon dioxide and water by aerobic metabolism of microorganisms present in manure manure.

In this aeration process, bubbles are generated, and the generated bubbles float above the aeration tank, and the generated bubbles are evaporated by the foam decomposition pump, that is, the aerator 84, installed above the aeration tank.

The efficiency of evaporation is remarkably increased when using a non-circular polygonal aerator 84, and 80% of the manure manure introduced into the aeration tank 80 is evaporated into the atmosphere, and the remaining 10-20% by volume is not decomposed. Non-foamed manure is conveyed to the secondary settling tank (91).

As the heat is generated during the aeration process, the normal temperature is maintained at 37 to 65 ° C., thereby removing the low-temperature pathogenic bacteria that may be present in the shaft, and preventing the generation of ammonia, whereas the aerobic microorganism is water and oxygen. In addition, the three major culture conditions of the temperature is increased its activity.

However, in order to maintain a constant temperature within the temperature 37 ~ 65 ℃ even in winter, it is preferable to supply the hot air generated through the heat generator using the blower pump 83 and the air inlet pipe 82. Do.

Such a heat supply method has an advantage that the temperature can be transferred together with oxygen using a conventional aeration device, but various methods may be considered in addition to the heat supply method.

To promote fermentation and aeration of microorganisms, fermentation microorganisms such as those found in feed additives can be added at the time of stated slurry storage, fermentation, and aeration.

The aerated manure is transported to the settling tank 90 and then separated into supernatant and a small amount of sediment (sludge). In order to more effectively separate the supernatant from the sediment, the secondary and tertiary settling tanks 92 and 93 may be passed.

At this time, some supernatant is evaporated. Only 10% of manure manure which has been transferred to the settling tank is precipitated in the first to third settling tanks.

This precipitate is collected about once a year and used for fermentation compost.

The discharged water discharged through the third settling tank 93 may be transferred to the storage tank 96, and the discharged water may be transferred to the barn along the transfer supply pipe 98 by an underwater pump 97 installed in the storage tank 96.

The distributor maintains the water discharged to the barn in the slurry tank 1 at the depth of 400 to 500 mm so that a certain amount can be continuously supplied from the slurry tank to the solid-liquid separator and then to the fermentation tank. The effluent can also be used as sodol water or liquid fertilizer (biofertilizer) in the house.

The method described above can be used to treat manure of all common livestock such as pigs, cattle and horses.

Hereinafter, preferred examples and comparative examples of the present invention are described. However, the examples are only one preferred embodiment of the present invention and the present invention is not limited to the following examples.

<Example 1>

Mycological Properties of Bacillus subtilis CP220 KCTC 8831P

The bacteriological properties of Bacillus subtilis CP220 distinguished from conventional strains are summarized in Table 1 below, where (-) indicates 90% or more negative and (+) 90% Or more is positive and d represents 11-89% positive.

Mycological Properties of Bacillus subtilis CP220 reaction Cell diameter> 1.0 ?? m - Spores round - Endospore + Gram dyeing + shape Rod Sporangium swollen d (+/-) Parasporal crvstals - Catalase + Vogues-Proskauer test + V-P broth at pH <6 V-P broth at pH> 7 d (+/-)- Acis from D-glucose L-arabinose D-xylose-mannite ++++ Gas from glucose - Hydrolysis of Casein Hydrolyzed Gelatin Hydrolyzed Starch +++ Use of citrate Use of propionate +- Degradation of Tyrosine - Deamination of Phenylalanine + Egg-yolk lecithinase d (+/-) Indole formation - NaCL and KCL request - Allantoin or urate requirements - Grows on nutrient broth5.7 at pH 6.8 ++ 2% grown in NaCL 5% grown in NaCL 7% grown in NaCL ++ d (+/-) Grow at 5 ° C Grow at 10 ° C Grow at 30 ° C Grow at 40 ° C Grow at 50 ° C Grow at 55 ° C Grow at 65 ° C -d ++ d-- Growth with lysozyme present d

<Example 2>

Preparation of Feed Additives Containing Fermented Microorganisms

a. Primary culture

Yeast (Saccharomyces cerevisiae) KCTC 1199, Lactohacillus acidophilus KCTC 3177, Bacillus subtilis CP220 (KCTC 8831P, deposited on September 29, 1997) and photosynthetic bacteria (Rhodospirillum rubrum) KCTC 1372, respectively.

Culture conditions of each microorganism are as follows.

Yeast medium composition was 0.3% Yeast extract, 0.3% Malt extract, 0.5% Peptone, 1% Dextrose and incubated at 25 ℃ for 2 days.

The media composition of Bacillus and Lactobacillus was Peptone 1.0%, Beef extract 1.0%, Yeast extract 0.5%, Glucose 2.0%, Sodium acetate 0.5%. Triammonium citrate 0.2%, MgSO 4 · 7H₂O 0.02%, MnSO 4 · 4H₂O 0.02%, and incubated at 37 ℃ for 2 days.

The media composition of photosynthetic bacteria was 0.1% K₂HPO₄, 0.05% MgSO4, 1.0% Yeast extract, and cultured at 26 ° C for 5 days.

b. Secondary culture

After mixing 28 microliters of 4 kinds of microorganisms firstly cultured in 1 ton of water with 40 kg of sugar, 20 kg of soybean powder, and 4 kg of skim steel together with the primary medium (to make the total amount of microorganisms inoculated about 1%) Incubated at 40 ℃ for 2 ~ 3 days.

An berth can be used instead of degreasing steel.

c. Tertiary culture

25 parts by weight of ocher, 30 parts by weight of degreasing steel, 20 parts by weight of activated carbon, 25 parts by weight of zeolite, and various other minerals, soybean flour, sugar, mugwort, alcoholic beverage, and salt jelly were added to the mixed culture after the secondary culture. The water content of the mixture was 40 to 45% and then fermented for about 6 to 7 days at about 40 ℃.

<Example 3>

The number of microorganisms was much higher in the feed additives prepared by culturing yeast, lactic acid bacteria, naphtha bacteria (KCTC 8831P), and photosynthetic bacteria than the feed additives prepared by culturing yeast, lactic acid bacteria, and naphtha bacteria in conventional culture methods. Table 2 shows the average number of bacteria obtained after several runs.

division Yeast (CFU / g) Bacillus Bacillus (CFU / g) Lactobacillus (CFU / g) Examples containing photosynthetic bacteria Comparative Example Incubated by a Conventional Method Without Photosynthetic Bacteria

<Example 4>

Livestock wastewater treatment result

Two to three kilograms per tonne of feed with bottled or hexagonal water were mixed with feed and then fed to pigs and treated with 24.00 pigs and livestock wastewater using the method and apparatus according to the present invention.

If the daily manure of pigs is 5.5 kg, the weight is 2 kg (water content 81.1%) and urine is 3.5 kg.

division Total manure Evaporation Recycled Stock Recycled Manure Weight (kg) 132.00 62.040 38.280 23.760 Ratio (weight ratio) 100 47 29 18

Although the removal rate of COD and BOD was relatively good in the conventional livestock wastewater treatment, the T-N value of the effluent was still high even though the livestock wastewater was treated by the conventional method due to the high nutrients in the livestock wastewater.

For example, the removal rate was 84.7% even when the activated sludge method, which has a relatively high T-N removal rate, was used.

On the contrary, when the livestock manure produced by the feed containing the microorganism of the present invention was fed into the livestock feed treatment device, the amount was 1.780 ppm, but the removal rate was more than 95% as 43 ppm in the urine in the storage tank.

As described above, according to the present invention, livestock manure is fermented by microorganisms, and about 50% of the manure is evaporated in the aeration apparatus, and the remaining 50% of the manure is fermented feed or washed water, high-quality liquid fertilizer, drinking water of livestock, and livestock. It is reused as water used in the manure treatment device to prevent the discharge of waste water due to manure.

In addition, by maintaining a constant temperature in the aeration tank and fermentation tank, low-temperature pathogens are killed and less ammonia is generated, so there is no odor, and the surrounding environment of the treatment facility can be improved, and the livestock manure treated with the present invention is free of pathogens. There are no germs or flies in the manure.

The manure treated by this method can prevent soil contamination due to arsenic, sulfur oxides, nitrous acid, bilatate nitrogen and sulfamic acid, and especially high T-N treatment effect.

Feeding livestock with feed, including newly developed Bacillus aeruginosa (KCTC 8831P), photosynthetic bacteria, lactic acid bacteria, and yeast, significantly reduces the components of ammonia and 2.3benzophenol, which are pathogenic bacteria and odors, and increases the efficiency of fermentation. Is increased.

In addition, the livestock raised in the feed in addition to the nutrient treatment effect has the effect of improving the digestibility.

This can shorten the days of breeding pigs.

Addition of photosynthetic bacteria in the preparation of animal feed additives, and in the first culture, each microorganism is cultured separately and then mixed culture in 2.3 culture to produce a feed additive containing a much larger number of useful bacteria Increased the efficiency of fermentation and aeration.

In addition, by selecting the materials to be added during the tertiary culture of the microorganisms and control of the mixing ratio of them, it is possible to easily ingest the livestock by generating a favorite odor in addition to the composition of a better microbial growth environment.

Claims (5)

delete delete delete a) Bacillus subtilis CP220 KCTC 8831P, photosynthetic bacteria (Rhodospirillum rubrum), lactobacillus (Lactohacillus acidophilus), and yeast (Saccharomyces cerevisiae) four microorganisms together with sugar, soybean powder, and skim steel or Incubating for 2-3 days in a culture medium containing berth: b) adding ocher, degreasing steel, activated carbon, zeolite to the mixed culture in which the culture is completed so that the water content of the mixture is 40 to 45%; And c) a method of producing a livestock feed additive comprising the microorganism comprising the step of fermenting the mixture at a temperature of about 40 ℃ for 6 to 7 days. Animal feed additive comprising the microorganism prepared by the method of claim 4.