[go: up one dir, main page]

JPS6479661A - Immunological analysis - Google Patents

Immunological analysis

Info

Publication number
JPS6479661A
JPS6479661A JP23852987A JP23852987A JPS6479661A JP S6479661 A JPS6479661 A JP S6479661A JP 23852987 A JP23852987 A JP 23852987A JP 23852987 A JP23852987 A JP 23852987A JP S6479661 A JPS6479661 A JP S6479661A
Authority
JP
Japan
Prior art keywords
inspected
substance
sample
liposome
antigen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP23852987A
Other languages
Japanese (ja)
Other versions
JP2604171B2 (en
Inventor
Noboru Iwase
Mamoru Umeda
Junko Akiyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nissui Pharmacetuical Co Ltd
Original Assignee
Nissui Pharmacetuical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nissui Pharmacetuical Co Ltd filed Critical Nissui Pharmacetuical Co Ltd
Priority to JP62238529A priority Critical patent/JP2604171B2/en
Publication of JPS6479661A publication Critical patent/JPS6479661A/en
Application granted granted Critical
Publication of JP2604171B2 publication Critical patent/JP2604171B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Dispersion Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicinal Preparation (AREA)

Abstract

PURPOSE:To enable accurate determination of a substance to be inspected in a sample to be inspected with a handy operation, by using a liposome containing phospholipid, cholesterol and a crosslinking agent as selected from phosphatidic acid or the like to remove a complement component from the sample being inspected effectively. CONSTITUTION:First, a liposome (pretreatment reagent) containing phospholipid, cholesterol and a crosslinking agent as selected from phosphatidic acid or the like is added to a sample to be inspected and allowed to stand at a room temperature for about 10min. As a result, an antigen or an antibody in the liposome reagent reacts with a substance to be inspected and a complement is activated by an antigen-antibody reaction to release labelled substance from the liposome in proportion to the level of the antigen-antibody reaction. Then, a determination is performed by an analysis method corresponding to the labelled substance and, for example, the amount of the substance being inspected in the sample can be measured according to a calibration curve prepared beforehand. This can remove a complement component in the substance being inspected effectively thereby enabling accurate determination of the substance being inspected.
JP62238529A 1987-09-22 1987-09-22 Immunoassay method Expired - Lifetime JP2604171B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62238529A JP2604171B2 (en) 1987-09-22 1987-09-22 Immunoassay method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62238529A JP2604171B2 (en) 1987-09-22 1987-09-22 Immunoassay method

Publications (2)

Publication Number Publication Date
JPS6479661A true JPS6479661A (en) 1989-03-24
JP2604171B2 JP2604171B2 (en) 1997-04-30

Family

ID=17031608

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62238529A Expired - Lifetime JP2604171B2 (en) 1987-09-22 1987-09-22 Immunoassay method

Country Status (1)

Country Link
JP (1) JP2604171B2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03152467A (en) * 1989-11-09 1991-06-28 Nitsusui Seiyaku Kk Hbs antibody measuring reagent
JPH03152466A (en) * 1989-11-09 1991-06-28 Nitsusui Seiyaku Kk Absorption of non-specific reaction for immuno assay
US5654156A (en) * 1992-01-22 1997-08-05 Wako Pure Chemical Industries, Ltd. Immunoassay using liposomes

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56132564A (en) * 1980-02-04 1981-10-16 Koraboreiteibu Research Inc Product for and method of immunity analysis
JPS6078350A (en) * 1983-09-01 1985-05-04 テクニコン、インストルメンツ、コ−ポレ−シヨン Reverse latency specific binding assay
JPS60138466A (en) * 1983-12-27 1985-07-23 Denka Seiken Co Ltd Novel method for quantitative determination of antigen
JPS6153568A (en) * 1984-08-06 1986-03-17 テクニコン、インストルメンツ、コーポレーシヨン Liposome compatible surfactant
JPS61133863A (en) * 1984-12-04 1986-06-21 Toshiba Corp Immunological analyzer
JPS61269070A (en) * 1984-12-13 1986-11-28 Nitsusui Seiyaku Kk Quantitative analysis of antigen
JPS63309864A (en) * 1987-06-11 1988-12-16 Kanegafuchi Chem Ind Co Ltd High-sensitivity immunological assay method by using liposome

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56132564A (en) * 1980-02-04 1981-10-16 Koraboreiteibu Research Inc Product for and method of immunity analysis
JPS6078350A (en) * 1983-09-01 1985-05-04 テクニコン、インストルメンツ、コ−ポレ−シヨン Reverse latency specific binding assay
JPS60138466A (en) * 1983-12-27 1985-07-23 Denka Seiken Co Ltd Novel method for quantitative determination of antigen
JPS6153568A (en) * 1984-08-06 1986-03-17 テクニコン、インストルメンツ、コーポレーシヨン Liposome compatible surfactant
JPS61133863A (en) * 1984-12-04 1986-06-21 Toshiba Corp Immunological analyzer
JPS61269070A (en) * 1984-12-13 1986-11-28 Nitsusui Seiyaku Kk Quantitative analysis of antigen
JPS63309864A (en) * 1987-06-11 1988-12-16 Kanegafuchi Chem Ind Co Ltd High-sensitivity immunological assay method by using liposome

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03152467A (en) * 1989-11-09 1991-06-28 Nitsusui Seiyaku Kk Hbs antibody measuring reagent
JPH03152466A (en) * 1989-11-09 1991-06-28 Nitsusui Seiyaku Kk Absorption of non-specific reaction for immuno assay
US5654156A (en) * 1992-01-22 1997-08-05 Wako Pure Chemical Industries, Ltd. Immunoassay using liposomes

Also Published As

Publication number Publication date
JP2604171B2 (en) 1997-04-30

Similar Documents

Publication Publication Date Title
EP0874240B1 (en) Enzyme-labeled immunoassay and device therefor
EP1072887A3 (en) Immunoassay
AU3419189A (en) Device and process for high-speed qualitative and quantitative determination of the presence of a reactive ligand in a fluid
US3893808A (en) Method and paper test strip for determining low levels of lead in hydrocarbon fuels
NO175657C (en) Method for performing an immunological determination of an antigen in a liquid sample as well as reagents for use therein.
EP0355849A3 (en) Method and apparatus for automatic measurement of fluorescence
ATE80735T1 (en) SOLID-SURFACE COLORIMETRIC IMMUNOESSAY, TEST ROD TO BE USED WITH IT AND ITS PREPARATION.
GB1436934A (en) Method of testing blood
JPS6488155A (en) Immunological inspection for detecting antibody against antigen
IL58054A0 (en) Process for immunologic tests
JPS6447953A (en) Method and apparatus for separating labelled reagent for testing immunological bond measuring test
SE8102558L (en) IMMUNOLOGICAL DETERMINATION METHOD
ATE58600T1 (en) TEST PROCEDURE.
ES481519A1 (en) Apparatus for luminescent determination of concentration of an analyte in a sample.
IE822123L (en) Method of determining the concentration of pregnanediol¹glucuronide in a woman's urine and test device for use¹therein
IE841371L (en) Carrier matrix analytical test device
DK314987D0 (en) PROCEDURE FOR SPECIFIC DETERMINATION OF SERUM FRUCTOSAMINE CONTENT AND ITS SUITABLE REAGENT MIXTURE
US5079141A (en) Apparatus and method for performing chemical analyses and immunoassays
DK516387D0 (en) PROCEDURE FOR IMMUNOMETRIC DETERMINATION
JPS6479661A (en) Immunological analysis
JPS5748658A (en) Immunologic measuring method and reagent for measurement
DE3669078D1 (en) METHOD FOR DETERMINING AN IMMUNOLOGICALLY BINDABLE SUBSTANCE.
EP0028133A3 (en) Method of detecting and quantitating haptens and antigens
JPS6444855A (en) Removal of non-specific turbidity
JPS579723A (en) Stabilizing agent for immunological reaction and measuring method of antigen-antibody reaction