JPS6460385A - Production of e.coli protease iii and novel plasmid vector - Google Patents
Production of e.coli protease iii and novel plasmid vectorInfo
- Publication number
- JPS6460385A JPS6460385A JP21897387A JP21897387A JPS6460385A JP S6460385 A JPS6460385 A JP S6460385A JP 21897387 A JP21897387 A JP 21897387A JP 21897387 A JP21897387 A JP 21897387A JP S6460385 A JPS6460385 A JP S6460385A
- Authority
- JP
- Japan
- Prior art keywords
- plasmid
- coli
- protease iii
- fragment
- hindiii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000588724 Escherichia coli Species 0.000 title abstract 4
- 108091005804 Peptidases Proteins 0.000 title 1
- 239000004365 Protease Substances 0.000 title 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title 1
- 239000013600 plasmid vector Substances 0.000 title 1
- 239000013612 plasmid Substances 0.000 abstract 7
- 239000012634 fragment Substances 0.000 abstract 6
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 108090000316 Pitrilysin Proteins 0.000 abstract 2
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 108091008146 restriction endonucleases Proteins 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE:To produce a protease III in high efficiency, by inserting a DNA fragment containing ptr gene into E.coli-originated plasmid, substituting the ptr promoter with tac promoter and introducing the obtained plasmid into E.coli. CONSTITUTION:Plasmid pV185 is digested with restriction enzyme HindIII to obtain a DNA fragment containing ptr gene. The fragment is linked with pUC19 digested with HindIII to obtain pUC67. A SalI-HpaI fragment originated from E.coli chromosome and positioned at the 5'-side of the ptr gene is removed from the plasmid to obtain pUC42. The HindIII site of the plasmid is converted to SalI site to obtain pUC42. pDR42S is produced by substituting the ECoRI- BamHI fragment of the resultant plasmid with the ECoRI-BamHI fragment of pDR540 having tac promoter. The obtained plasmid is introduced into an E.coli and the obtained transformant is cultured in a medium to produce the objective protease III.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62218973A JP2518862B2 (en) | 1987-08-31 | 1987-08-31 | New plasmid vector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62218973A JP2518862B2 (en) | 1987-08-31 | 1987-08-31 | New plasmid vector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6460385A true JPS6460385A (en) | 1989-03-07 |
| JP2518862B2 JP2518862B2 (en) | 1996-07-31 |
Family
ID=16728262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62218973A Expired - Lifetime JP2518862B2 (en) | 1987-08-31 | 1987-08-31 | New plasmid vector |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2518862B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992008736A1 (en) * | 1990-11-08 | 1992-05-29 | Nippon Mining Company Limited | Hirudine mutant, production thereof, anticoagulant, secretory vector, microorganism transformed by said vector, and production of product from said microorganism |
-
1987
- 1987-08-31 JP JP62218973A patent/JP2518862B2/en not_active Expired - Lifetime
Non-Patent Citations (2)
| Title |
|---|
| J.BACTERIOL=1984 * |
| J.BACTERIOL=1985 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992008736A1 (en) * | 1990-11-08 | 1992-05-29 | Nippon Mining Company Limited | Hirudine mutant, production thereof, anticoagulant, secretory vector, microorganism transformed by said vector, and production of product from said microorganism |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2518862B2 (en) | 1996-07-31 |
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