JPS6439555A - Simple device for stabilized immune detection and immune detection - Google Patents
Simple device for stabilized immune detection and immune detectionInfo
- Publication number
- JPS6439555A JPS6439555A JP19453087A JP19453087A JPS6439555A JP S6439555 A JPS6439555 A JP S6439555A JP 19453087 A JP19453087 A JP 19453087A JP 19453087 A JP19453087 A JP 19453087A JP S6439555 A JPS6439555 A JP S6439555A
- Authority
- JP
- Japan
- Prior art keywords
- layer
- antibody
- light
- light interference
- immune detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Composite Materials (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
PURPOSE:To obtain an immune detection device which obviates the exfoliation from a substrate and has high sensitivity by providing a light interference layer which is optimized in thickness and refractive index and chemically bonding an antibody layer selectively to the surface of the light interference layer except in the recognized active section of an antibody. CONSTITUTION:This simple device for immune detection is constituted of the light reflective substrate 1 which consists of gold, silver, etc., and has substantially no irregular reflection, the light interference layer 2 which is laminated on the substrate 1, a reactive intermediate layer 3 which is provided on the light interference layer 2 and consists of a compd. reacting selectively with the carboxyl group or thiol group contained in the antibody molecules or fragmented antibody molecules mainly by pH adjustment, and a layer 4 of an antigen material and/or antibody protein which is provided on the intermediate layer 3 and consists substantially of a monomolecular layer. The light interference layer 2 has no reflection characteristic to visible light and the increase in the thickness arising from the antigen-antibody reaction of the antibody protein layer appears as a change in the interference color of light. Further, the surface of said layer is required to have sufficient affinity or reactivity to or with the compd. forming the intermediate layer 3 and is formed by controlling long chain carboxylic acid and the metal salt thereof or silicon oxide, etc., to a desired film thickness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19453087A JPS6439555A (en) | 1987-08-05 | 1987-08-05 | Simple device for stabilized immune detection and immune detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19453087A JPS6439555A (en) | 1987-08-05 | 1987-08-05 | Simple device for stabilized immune detection and immune detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6439555A true JPS6439555A (en) | 1989-02-09 |
Family
ID=16326068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19453087A Pending JPS6439555A (en) | 1987-08-05 | 1987-08-05 | Simple device for stabilized immune detection and immune detection |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6439555A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6359432B1 (en) | 1998-02-26 | 2002-03-19 | Alps Electric Co., Ltd. | Connector and turning angle sensor using same |
| EP2026060A1 (en) * | 2003-11-06 | 2009-02-18 | Fortebio, Inc | Optical detection apparatus based on phase-shift interferometry |
| US8647588B2 (en) | 2005-06-13 | 2014-02-11 | Pall Corporation | Tip tray assembly for optical sensors |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58195142A (en) * | 1982-04-26 | 1983-11-14 | サガクス・インストルメント・アクチエボラ−グ | Detection and (or) density measuring method for laminate and chemical substance using said laminate |
| JPS61181966A (en) * | 1984-12-06 | 1986-08-14 | ユ−オ−ピ− インコ−ポレイテツド | Stabilizing antibody coupled by carboxyl group |
-
1987
- 1987-08-05 JP JP19453087A patent/JPS6439555A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58195142A (en) * | 1982-04-26 | 1983-11-14 | サガクス・インストルメント・アクチエボラ−グ | Detection and (or) density measuring method for laminate and chemical substance using said laminate |
| JPS61181966A (en) * | 1984-12-06 | 1986-08-14 | ユ−オ−ピ− インコ−ポレイテツド | Stabilizing antibody coupled by carboxyl group |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6359432B1 (en) | 1998-02-26 | 2002-03-19 | Alps Electric Co., Ltd. | Connector and turning angle sensor using same |
| EP2026060A1 (en) * | 2003-11-06 | 2009-02-18 | Fortebio, Inc | Optical detection apparatus based on phase-shift interferometry |
| US8305585B2 (en) | 2003-11-06 | 2012-11-06 | Pall Corporation | Fiber-optic assay apparatus based on phase-shift interferometry |
| US8647588B2 (en) | 2005-06-13 | 2014-02-11 | Pall Corporation | Tip tray assembly for optical sensors |
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