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JPS6434286A - Production of luciferase - Google Patents

Production of luciferase

Info

Publication number
JPS6434286A
JPS6434286A JP18772487A JP18772487A JPS6434286A JP S6434286 A JPS6434286 A JP S6434286A JP 18772487 A JP18772487 A JP 18772487A JP 18772487 A JP18772487 A JP 18772487A JP S6434286 A JPS6434286 A JP S6434286A
Authority
JP
Japan
Prior art keywords
afford
dna
luciferase
luc
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP18772487A
Other languages
Japanese (ja)
Inventor
Tsutomu Masuda
Hiroki Tatsumi
Eiichi Nakano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kikkoman Corp
Original Assignee
Kikkoman Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kikkoman Corp filed Critical Kikkoman Corp
Priority to JP18772487A priority Critical patent/JPS6434286A/en
Priority to US07/224,445 priority patent/US4968613A/en
Priority to EP88112233A priority patent/EP0301541B1/en
Priority to DE3887223T priority patent/DE3887223T2/en
Publication of JPS6434286A publication Critical patent/JPS6434286A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To efficiently obtain luciferase useful as an enzyme for determining adenosine triphosphate (ATP), etc., in a short time, by cultivating a specific microorganism in a culture medium. CONSTITUTION:A DNA capable of coding luciferase (LUC) derived from a Photinus.pyralis which is a species of firefly and c-DNA derived from Luciola.cruciata which is 'GENJI BOTARU' are hybridized to provide a plasmid DNA (A) containing the c-DNA capable of coding LUC of about 2.0 kb. The resultant component (A) is then treated with a restriction enzyme to afford a DNA, which is subsequently integrated into a plasmid pUC18 to afford a recombinant DAN (B). The obtained component (B) is then introduced into Escherichia coli JM101 of the genus Escherichia, etc., to provide a microorganism (C) having the ability to produce LUC. The resultant strain (C) is subsequently cultivated in a culture medium containing trypton, etc., at 30-40 deg.C for 4-8hr and centrifuged to afford microbial cells, which are crushed to provide a crude enzyme liquid (D). The obtained liquid (D) is then subjected to purification treatment, such as fractionation with sodium sulfate or gel filtration, to afford the aimed purified luciferase.
JP18772487A 1987-07-29 1987-07-29 Production of luciferase Pending JPS6434286A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP18772487A JPS6434286A (en) 1987-07-29 1987-07-29 Production of luciferase
US07/224,445 US4968613A (en) 1987-07-29 1988-07-26 Luciferase gene and novel recombinant DNA as well as a method of producing luciferase
EP88112233A EP0301541B1 (en) 1987-07-29 1988-07-28 Luciferase gene and novel recombinant DNA as well as a method of producing luciferase
DE3887223T DE3887223T2 (en) 1987-07-29 1988-07-28 Luciferase gene and new recombinant DNA and a process for the production of luciferase.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP18772487A JPS6434286A (en) 1987-07-29 1987-07-29 Production of luciferase

Publications (1)

Publication Number Publication Date
JPS6434286A true JPS6434286A (en) 1989-02-03

Family

ID=16211070

Family Applications (1)

Application Number Title Priority Date Filing Date
JP18772487A Pending JPS6434286A (en) 1987-07-29 1987-07-29 Production of luciferase

Country Status (1)

Country Link
JP (1) JPS6434286A (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6143998A (en) * 1984-06-05 1986-03-03 アマーシャム　インターナショナル　パブリック　リミティド　カンパニー Detection of bacteria in environment

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6143998A (en) * 1984-06-05 1986-03-03 アマーシャム　インターナショナル　パブリック　リミティド　カンパニー Detection of bacteria in environment

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