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JPS642598A - Method for measuring calcium - Google Patents

Method for measuring calcium

Info

Publication number
JPS642598A
JPS642598A JP15758787A JP15758787A JPS642598A JP S642598 A JPS642598 A JP S642598A JP 15758787 A JP15758787 A JP 15758787A JP 15758787 A JP15758787 A JP 15758787A JP S642598 A JPS642598 A JP S642598A
Authority
JP
Japan
Prior art keywords
oxalate
calcium
chromogen
sample solution
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP15758787A
Other languages
Japanese (ja)
Other versions
JPH012598A (en
Inventor
Shigeki Shiozawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Iatron Laboratories Inc
Mitsubishi Kagaku Iatron Inc
Original Assignee
Iatron Laboratories Inc
Mitsubishi Kagaku Iatron Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=15652964&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JPS642598(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Iatron Laboratories Inc, Mitsubishi Kagaku Iatron Inc filed Critical Iatron Laboratories Inc
Priority to JP15758787A priority Critical patent/JPS642598A/en
Publication of JPH012598A publication Critical patent/JPH012598A/en
Publication of JPS642598A publication Critical patent/JPS642598A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To exclude an influence of Mg and readily determine Ca with good accuracy without deproteinization, by converting Ca into oxalate, precipitating the oxalate, oxidizing the excess oxalate with an enzyme, decomposing the generated H2O2 with an enzyme, coloring a chromogen and carrying out colorimetry.
CONSTITUTION: A reagent for measurement containing sodium oxalate reagent, oxalic oxidase, peroxidase and a chromogen (e.g. 3-methl-2- benzothiazolinonehydrazone) is added to a sample solution (e.g. blood serum) to react the calcium in the sample solution with the oxalic acid reagent and precipitate the calcium as oxalate. The excess oxalic acid is then oxidized with oxalic oxidase and the generated hydrogen peroxide is subsequently decomposed with peroxidase to oxidize and color the chromogen. Colorimetry is subsequently carried out to determine the amount of the calcium in the sample solution.
COPYRIGHT: (C)1989,JPO&Japio
JP15758787A 1987-06-26 1987-06-26 Method for measuring calcium Pending JPS642598A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15758787A JPS642598A (en) 1987-06-26 1987-06-26 Method for measuring calcium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15758787A JPS642598A (en) 1987-06-26 1987-06-26 Method for measuring calcium

Publications (2)

Publication Number Publication Date
JPH012598A JPH012598A (en) 1989-01-06
JPS642598A true JPS642598A (en) 1989-01-06

Family

ID=15652964

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15758787A Pending JPS642598A (en) 1987-06-26 1987-06-26 Method for measuring calcium

Country Status (1)

Country Link
JP (1) JPS642598A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02224227A (en) * 1989-02-25 1990-09-06 Sony Corp Cvd apparatus
US5369219A (en) * 1991-06-18 1994-11-29 Multimedia Design, Inc. Multi-layer printed circuit board apparatus and method for making same
US5618684A (en) * 1992-02-07 1997-04-08 Oriental Yeast Co., Ltd. Method of determination of calcium
EP0776979A1 (en) 1995-11-28 1997-06-04 Oriental Yeast Co., Ltd. Method and reagent for measuring an ion by using maltose derivatives

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02224227A (en) * 1989-02-25 1990-09-06 Sony Corp Cvd apparatus
US5369219A (en) * 1991-06-18 1994-11-29 Multimedia Design, Inc. Multi-layer printed circuit board apparatus and method for making same
US5618684A (en) * 1992-02-07 1997-04-08 Oriental Yeast Co., Ltd. Method of determination of calcium
EP0776979A1 (en) 1995-11-28 1997-06-04 Oriental Yeast Co., Ltd. Method and reagent for measuring an ion by using maltose derivatives
US5948632A (en) * 1995-11-28 1999-09-07 Oriental Yeast Co., Ltd. Method and reagent for measuring chlorine and calcium ions using a maltose derivative

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