JPS6423896A - Method for enzymic reaction using microbial cell - Google Patents
Method for enzymic reaction using microbial cellInfo
- Publication number
- JPS6423896A JPS6423896A JP62180623A JP18062387A JPS6423896A JP S6423896 A JPS6423896 A JP S6423896A JP 62180623 A JP62180623 A JP 62180623A JP 18062387 A JP18062387 A JP 18062387A JP S6423896 A JPS6423896 A JP S6423896A
- Authority
- JP
- Japan
- Prior art keywords
- microorganism
- water
- washed
- microbial cells
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE:To utilize a microorganism having the ability to produce lipase for lipase enzymic reaction, by cultivating the microorganism, washing the aforementioned microorganism with water or directly using the microorganism or replacing water with a polar solvent and then nonpolar solvent in using for ester interchange or synthetic reaction in an oil system. CONSTITUTION:A microorganism having the ability to produce lipase is cultivated in a culture medium, filtered off and washed with water several times to remove culture medium components, etc., contained in the microorganism. When the microorganism is utilized for hydrolytic reaction of fats and oils or ester synthetic reaction of glycerol or other alcohols with fatty acids, the microbial cells washed with water are directly used as a catalyst. When the microorganism is used for ester interchange or ester synthetic reaction in an oil system, the microbial cells washed with water are washed with a polar solvent, such as acetone, to replace water contained in the microbial cells with the polar solvent and dehydrate the microbial cells. Furthermore, the dehydrated microbial cells are washed with a nonpolar solvent, such as hexane, to replace the polar solvent therewith and enzymic reaction is carried out utilizing lipase contained in the above-mentioned microorganism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62180623A JPS6423896A (en) | 1987-07-20 | 1987-07-20 | Method for enzymic reaction using microbial cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62180623A JPS6423896A (en) | 1987-07-20 | 1987-07-20 | Method for enzymic reaction using microbial cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6423896A true JPS6423896A (en) | 1989-01-26 |
Family
ID=16086448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62180623A Pending JPS6423896A (en) | 1987-07-20 | 1987-07-20 | Method for enzymic reaction using microbial cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6423896A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7087418B2 (en) | 2001-12-19 | 2006-08-08 | Bristol-Myers Squibb Company | Pichia pastoris formate dehydrogenase and uses therefor |
-
1987
- 1987-07-20 JP JP62180623A patent/JPS6423896A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7087418B2 (en) | 2001-12-19 | 2006-08-08 | Bristol-Myers Squibb Company | Pichia pastoris formate dehydrogenase and uses therefor |
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