JPS6344816A - Tissue culture apparatus - Google Patents

Abstract

(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

Detailed Description of the Invention (Industrial Field of Application) This invention relates to a cell culture device, and particularly relates to a cell culture device for plant Mi
The present invention relates to a culture solution exchange device suitable for use when raising seedlings by nutrient solution culture without using an agar medium when breeding and propagating plants by textile culture.

It goes without saying that this method can also be applied to tissue culture of animal cells.

(Prior Art) Conventional tissue culture mainly uses agar as shown in FIG.

Figure 4 schematically shows Mi tissue culture using an agar medium containing nutrients. Plant tissues cut and taken out from plants are placed on an agar medium in a container, multiplied, and then transplanted. The seeds were then germinated on a germination agar medium, further divided and canted, transplanted and rooted in a separate container using a rooting agar medium, taken out and washed with agar, then transferred to an acclimation device, protected from light and humid. This process encourages re-rooting and acclimatizes to the external environment.

(Problems to be Solved by the Invention) The above-mentioned agar that is used as a medium for tissue culture changes the nutritional conditions according to the production of the plant.
Repotting is carried out under sterile conditions, i.e. when sprouting from plant tissues such as callus and growing points, and when rooting.

Since the required nutrients differ, it is necessary to change the agar medium.

Furthermore, in order to grow a germinated and rooted plant, it is necessary to transfer it to a #H-free medium and acclimatize it to the environmental conditions, which also requires transplanting.

Cultivation is also carried out on media other than agar media, such as using filter paper, but in this case too, it is necessary to transplant according to the growth of the plant. Since nutrients are absorbed as they grow, they will grow better if transplanted every few weeks, but this requires a great deal of labor and sterile manual transplantation.

When transplanted on an agar medium, the operation damages the plants, especially their roots, which hinders their growth, and the plants are often contaminated with bacteria, making them unusable.

Therefore, it is necessary to propagate plants, which is extremely expensive using conventional methods.
It was only used for special purposes such as virus-free.

This invention aims to solve the above problems.

(Means and effects for solving the problems) The present invention has been made in view of the above problems, and has a porosity such as a nonwoven fabric in a culture box equipped with a nutrient solution supply port, a ventilation hole, and a drainage hole. A capillary body made of a material with a small porosity and a medium support material (solid medium) made of a material with a high porosity placed on the capillary body are housed, and the nutrient solution contained in the medium support material is placed in the culture box. In a culture device using a culture medium support material and a nutrient solution medium, the culture solution is quickly removed from the culture medium support material containing the culture solution using capillary action when changing the nutrient solution components. This allows the culture medium to be replaced with a new one.

(Example) Embodiments of the present invention will be described below based on the drawings.

Figure 1 shows the main parts of a culturing apparatus according to an embodiment of the present invention. 1 is a cover that is necessary for keeping the culture box A sterile, and is transparent and allows the light to pass through the plant. Helps grow. 2 is a main body in which a capillary body 3 and a solid medium 4 are housed. The capillary body 3 is made of a water absorbing material such as nonwoven fabric. A plant body 5 is planted on the solid medium 4, and although it is shown as a seedling in Figure 1, it may be a growing point, a callus, or an primordium. 6,
6' is a ventilation hole that exchanges the gas in the culture vessel in a sterile state to maintain a gas environment suitable for fresh salmon. Nutrient solution 8 is supplied in a sterile manner through a liquid supply port. The water contained in the solid medium 4 is discharged through the drain hole 7 when the valve 9 is opened.

At this time, if the height indicated by H is greater than the inherent capillary rise height of the capillary body 3, the water in the solid medium 4 will be rapidly drained through the capillary body 3. ” 2. Solid medium 4 is polyester wool or ceramic wool.

It is made of cellulose and has a relatively large porosity.

On the other hand, the capillary body 3 made of non-woven fabric or the like placed below the capillary body 3 has a small porosity, and this non-woven fabric is supported by a metal net 10 or the like. Due to the difference in porosity between the solid medium 4 and the capillary body 3, water contained in the solid medium 4 moves toward the capillary body 3, which is a nonwoven fabric, by capillary action. The capillary body may be made of ceramic, but it is better to use dense fibers.

In the embodiment shown in FIG. 1, capillary drainage is performed using gravity, and a similar embodiment is shown in FIG.

In FIG. 2, capillary bodies 3 are extended downward on both sides of the incubator, and the discharge amount per hour is greater than that in FIG. 1.

The liquid supply port 8 supplies the nutrient solution aseptically. Note that the liquid may be supplied through the liquid drain hole 7, but in that case, the residual liquid is likely to mix with the new liquid, and in order to efficiently exchange the liquid, it is better to provide a separate liquid supply port.

Although neither the one in Figure 1 nor the one in Figure 2 is shown in the three figures, it is possible to control the temperature, gas, humidity, etc. of the culture box.

In the capillary gravity drainage method described above, even if there are some air bubbles, if there is even a slight capillary connection, the liquid will continue to drain from the solid medium 4, so the amount of liquid that will ultimately remain on the solid medium 4 will be extremely small. becomes. The solid medium 4 can simply be placed on the capillary body 3 and does not require airtightness.

FIG. 3 shows another embodiment, in which liquid is drained using a suction method using negative pressure. The water that has soaked into the nonwoven fabric 3 is drawn out using a vacuum pump, and when the liquid is sucked out from the valve 9 side with negative pressure, the liquid is discharged while the two capillary bodies 11 and the main body 2 are in close contact with each other. If even one passage is created, the suction effect will be lost and the fluid will not be discharged. Therefore, in the case of suction, it is sufficient to perform suction at a speed lower than the capillary movement speed so that the capillary body 11 is evenly filled with liquid.

As mentioned above, in each of the embodiments of the present invention, in order to eliminate the transplantation of plants, a solid medium (medium support material) is used.
Using a nutrient solution to supply nutrients, the plant body is supported on a solid medium, nutritional conditions are changed by replacing only the nutrient solution, and the nutrient solution before replacement is quickly removed using a capillary drainage method. Can be replaced with new culture medium.

It goes without saying that if chemically stable polyester wool or glass wool is used as the solid medium, it can also be applied to tissue culture of animal cells.

(Effect of the invention) This invention is constructed as described above in detail.

This allows you to quickly remove the culture medium from the solid medium containing the culture medium and replace it with a new culture medium, eliminating the need for transplanting at all, saving you a lot of time and effort and preventing damage to the roots. This reduces the time and cost of producing seedlings from plant tissue.

This allows for high-speed growth and efficient development of new varieties.

Furthermore, this invention can be applied not only to plants but also to the cultivation of adherent animal cells, and has a wide range of uses.

[Brief explanation of the drawing]

FIG. 1 shows an embodiment of the main part of this invention, and FIG. 2 shows another embodiment using the same drainage system as in FIG. 1. FIG. 3 is an example using another drainage method, and FIG. 4 is an explanatory diagram of an example of conventional tissue culture. A...Culture box, 1...Cover, 2...Main body. 3... Capillary body, 4... Solid medium. 6.6°...Vent hole, 7.7'...Drain hole. 8...Liquid supply port, 9...Valve. Patent applicant: Komatsu Ltd. Agent (patent attorney): Toru Matsuzawa 1.

Claims (3)

[Claims] (1) A capillary body made of a water-absorbing material with a low porosity is provided at the bottom of the culture box having a nutrient solution supply port, a ventilation hole, and a drainage hole, and a capillary body made of a water-absorbing material with a low porosity is placed on the capillary body. A tissue culture device, characterized in that the culture box is provided with a means for placing a medium support made of material and for removing a nutrient solution contained in the medium support. (2) The means for removing the nutrient solution contained in the solid medium is
The tissue culture device according to claim 1, comprising a drainage hole located below the bottom of the culture box and a valve provided in front of the drainage hole. (3) The tissue culture apparatus according to claims 1 and 2, wherein means for removing the nutrient solution contained in the solid medium is provided on both sides of the culture box.