JPS6341425B2 - - Google Patents

Info

Publication number: JPS6341425B2
Authority: JP; Japan
Prior art keywords: glucagon; fragment; arg; conjugate; add
Prior art date: 1980-10-23
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP55148732A

Other languages

English (en)

Japanese (ja)

Other versions

JPS5681450A (en

Inventor

Nobuaki Nakagawa

Kikuo Kotani

Kunio Ooyama

Ko Morita

Shigeo Kuzuki

Toshiharu Noda

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Toyo Jozo KK

Original Assignee

Toyo Jozo KK

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1980-10-23

Filing date

1980-10-23

Publication date

1988-08-17

1980-10-23 Application filed by Toyo Jozo KK filed Critical Toyo Jozo KK

1980-10-23 Priority to JP14873280A priority Critical patent/JPS5681450A/ja

1981-07-03 Publication of JPS5681450A publication Critical patent/JPS5681450A/ja

1988-08-17 Publication of JPS6341425B2 publication Critical patent/JPS6341425B2/ja

Status Granted legal-status Critical Current

Links

MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 claims description 113
102000051325 Glucagon Human genes 0.000 claims description 53
108060003199 Glucagon Proteins 0.000 claims description 53
238000006243 chemical reaction Methods 0.000 claims description 47
229960004666 glucagon Drugs 0.000 claims description 46
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238000000034 method Methods 0.000 claims description 21
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 16
WREDNSAXDZCLCP-UHFFFAOYSA-N methanedithioic acid Chemical compound SC=S WREDNSAXDZCLCP-UHFFFAOYSA-N 0.000 claims description 15
239000003431 cross linking reagent Substances 0.000 claims description 12
102000004169 proteins and genes Human genes 0.000 claims description 9
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XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 6
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125000006850 spacer group Chemical group 0.000 claims description 3
IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims 1
ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 68
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 61
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LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 39
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OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 30
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XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 21
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 18
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
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CWERGRDVMFNCDR-UHFFFAOYSA-N thioglycolic acid Chemical compound OC(=O)CS CWERGRDVMFNCDR-UHFFFAOYSA-N 0.000 description 14
QMMFVYPAHWMCMS-UHFFFAOYSA-N Dimethyl sulfide Chemical compound CSC QMMFVYPAHWMCMS-UHFFFAOYSA-N 0.000 description 12
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ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Chemical compound C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 description 10
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RDOXTESZEPMUJZ-UHFFFAOYSA-N anisole Chemical compound COC1=CC=CC=C1 RDOXTESZEPMUJZ-UHFFFAOYSA-N 0.000 description 6
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239000012141 concentrate Substances 0.000 description 6
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229910000040 hydrogen fluoride Inorganic materials 0.000 description 6
230000008105 immune reaction Effects 0.000 description 6
239000002609 medium Substances 0.000 description 6
XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 description 6
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WYURNTSHIVDZCO-UHFFFAOYSA-N tetrahydrofuran Substances C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 6
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 5
FHOAKXBXYSJBGX-YFKPBYRVSA-N (2s)-3-hydroxy-2-[(2-methylpropan-2-yl)oxycarbonylamino]propanoic acid Chemical compound CC(C)(C)OC(=O)N[C@@H](CO)C(O)=O FHOAKXBXYSJBGX-YFKPBYRVSA-N 0.000 description 5
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OAYLNYINCPYISS-UHFFFAOYSA-N ethyl acetate;hexane Chemical compound CCCCCC.CCOC(C)=O OAYLNYINCPYISS-UHFFFAOYSA-N 0.000 description 5
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229940074386 skatole Drugs 0.000 description 5
239000011780 sodium chloride Substances 0.000 description 5
YEDUAINPPJYDJZ-UHFFFAOYSA-N 2-hydroxybenzothiazole Chemical compound C1=CC=C2SC(O)=NC2=C1 YEDUAINPPJYDJZ-UHFFFAOYSA-N 0.000 description 4
USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 4
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CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical group OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
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239000004473 Threonine Substances 0.000 description 4
VXDSLUMUNWTSDB-UHFFFAOYSA-N acetic acid;chloroform;methanol Chemical compound OC.CC(O)=O.ClC(Cl)Cl VXDSLUMUNWTSDB-UHFFFAOYSA-N 0.000 description 4
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SAKUKAQFBMDKGX-UHFFFAOYSA-N butan-1-ol;2-pyridin-2-ylacetic acid;hydrate Chemical compound O.CCCCO.OC(=O)CC1=CC=CC=N1 SAKUKAQFBMDKGX-UHFFFAOYSA-N 0.000 description 4
KZNICNPSHKQLFF-UHFFFAOYSA-N dihydromaleimide Natural products O=C1CCC(=O)N1 KZNICNPSHKQLFF-UHFFFAOYSA-N 0.000 description 4
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NPZTUJOABDZTLV-UHFFFAOYSA-N hydroxybenzotriazole Substances O=C1C=CC=C2NNN=C12 NPZTUJOABDZTLV-UHFFFAOYSA-N 0.000 description 4
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DHBXNPKRAUYBTH-UHFFFAOYSA-N 1,1-ethanedithiol Chemical compound CC(S)S DHBXNPKRAUYBTH-UHFFFAOYSA-N 0.000 description 3
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JBFYUZGYRGXSFL-UHFFFAOYSA-N imidazolide Chemical compound C1=C[N-]C=N1 JBFYUZGYRGXSFL-UHFFFAOYSA-N 0.000 description 1
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CZFNISFYDPIDNM-UHFFFAOYSA-N n,n-dimethylformamide;oxolane Chemical compound CN(C)C=O.C1CCOC1 CZFNISFYDPIDNM-UHFFFAOYSA-N 0.000 description 1
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229910052938 sodium sulfate Inorganic materials 0.000 description 1
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229960004295 valine Drugs 0.000 description 1
230000003442 weekly effect Effects 0.000 description 1

Landscapes

Peptides Or Proteins (AREA)

JP14873280A 1980-10-23 1980-10-23 Determination method for glucagon Granted JPS5681450A (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP14873280A JPS5681450A (en)	1980-10-23	1980-10-23	Determination method for glucagon

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP14873280A JPS5681450A (en)	1980-10-23	1980-10-23	Determination method for glucagon

Related Parent Applications (1)

Application Number	Title	Priority Date	Filing Date
JP13406179A Division JPS5657753A (en)	1979-10-16	1979-10-16	Novel glucagon fragment, and its use

Publications (2)

Publication Number	Publication Date
JPS5681450A JPS5681450A (en)	1981-07-03
JPS6341425B2 true JPS6341425B2 (da)	1988-08-17

Family

ID=15459362

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP14873280A Granted JPS5681450A (en)	1980-10-23	1980-10-23	Determination method for glucagon

Country Status (1)

Country	Link
JP (1)	JPS5681450A (da)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5539702A (en) *	1978-08-30	1980-03-19	Takeda Chem Ind Ltd	Method of measuring enzyme immunity of pancreas glucagon

1980
- 1980-10-23 JP JP14873280A patent/JPS5681450A/ja active Granted

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5539702A (en) *	1978-08-30	1980-03-19	Takeda Chem Ind Ltd	Method of measuring enzyme immunity of pancreas glucagon

Also Published As

Publication number	Publication date
JPS5681450A (en)	1981-07-03

Publication	Publication Date	Title
US4423034A (en)	1983-12-27	Process for the preparation of antibodies
FR2503145A1 (fr)	1982-10-08	Peptides apparentes aux interferons humains, anticorps obtenus a partir de ces peptides et adsorbants obtenus a partir de ces anticorps
US5173422A (en)	1992-12-22	Monoclonal antibodies specific for human glycoalbumin
US4407965A (en)	1983-10-04	Process for preparing antibody
EP0410303A1 (en)	1991-01-30	Monoclonal antibody to endothelin-3 or precursor thereof and use thereof
US5225354A (en)	1993-07-06	Monoclonal antibodies specific for human glycoalbumin
GB2062644A (en)	1981-05-28	Glucagon fragment and utility hereof
Wilner et al.	1976	Immunochemical studies of human fibrinopeptide A using synthetic peptide homologues
EP0360433A1 (en)	1990-03-28	Protein derivatives and a process for their preparation
US4409141A (en)	1983-10-11	Peptides for assaying human parathyroid hormone
EP0359428B1 (en)	1995-08-09	Protein derivatives and a process for their preparation
Huang et al.	1975	Calcium-binding protein of bovine intestine. The complete amino acid sequence.
EP0257421B1 (en)	1992-09-16	Antibodies for use in determining human glycoalbumin
JPS6311626B2 (da)	1988-03-15
JPS6341425B2 (da)	1988-08-17
JP2530714B2 (ja)	1996-09-04	フイブリンに対する抗体；抗体の製造に使用される免疫原、抗体及び抗体を基剤とした製薬学的製剤でフイブリンを測定する方法
JPH03163095A (ja)	1991-07-15	ヒト神経成長因子の部分ペプチド、抗体およびその用途
JPS6332145B2 (da)	1988-06-28
JPH0789991A (ja)	1995-04-04	新規なポリペプチドおよびそれを用いるｔｓｈ受容体抗体の測定法
JP2628336B2 (ja)	1997-07-09	ブラジキニン誘導体およびその定量
JP3419746B2 (ja)	2003-06-23	エンドセリン−３前駆体に対するモノクローナル抗体およびその用途
JP3167024B2 (ja)	2001-05-14	エンドセリン―３あるいはエンドセリン―３前駆体に対するモノクローナル抗体およびその用途
JPS63102700A (ja)	1988-05-07	ヒトのグリコアルブミンの定量において使用するための抗体
JPH0123059B2 (da)	1989-04-28
JPS61103899A (ja)	1986-05-22	インタ−フエロン−γ測定用ペプチド