JPS6336783A - Method for chemical reaction using porous material and device therefor - Google Patents
Method for chemical reaction using porous material and device thereforInfo
- Publication number
- JPS6336783A JPS6336783A JP18080086A JP18080086A JPS6336783A JP S6336783 A JPS6336783 A JP S6336783A JP 18080086 A JP18080086 A JP 18080086A JP 18080086 A JP18080086 A JP 18080086A JP S6336783 A JPS6336783 A JP S6336783A
- Authority
- JP
- Japan
- Prior art keywords
- reaction
- porous material
- chemical reaction
- porous
- bellows
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 56
- 239000011148 porous material Substances 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims description 7
- 239000000463 material Substances 0.000 claims abstract description 11
- 244000005700 microbiome Species 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 239000000427 antigen Substances 0.000 claims abstract description 4
- 102000036639 antigens Human genes 0.000 claims abstract description 4
- 108091007433 antigens Proteins 0.000 claims abstract description 4
- 230000006835 compression Effects 0.000 claims description 11
- 238000007906 compression Methods 0.000 claims description 11
- 230000003100 immobilizing effect Effects 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 9
- 241000186361 Actinobacteria <class> Species 0.000 description 5
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 239000012295 chemical reaction liquid Substances 0.000 description 4
- 239000006260 foam Substances 0.000 description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- 238000005842 biochemical reaction Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Mechanical Engineering (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は多孔性素材が反応に関わる化学反応方法及び装
置の改良に関するもので、特に酵素反応、微生物反応、
細胞組織による反応に有効に不興できる。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to improvements in chemical reaction methods and devices involving porous materials, particularly in enzymatic reactions, microbial reactions,
It can effectively disturb reactions caused by cellular tissues.
従来技術
多孔性担体に触媒、酵素・抗原類、微生物または動・植
物ｍ胞を付着または固定して化学反応をおこさせたり、
多孔性素材自身で化学反応原料であるような反応系は、
一般的には接触面積を大きくとったシ生産物の分離を容
易にすることに有効である。例えば三角フラスコに５１
角程度の発泡ウレタン立方体を担体として放線菌を撮と
り培養すると、放線菌はウレタン担体内で増殖するので
、微生物反応により生産された成分と微生物の分離が容
易であることが報告されている。また水素生産菌を多孔
性担体に固定して接触面積を大きくする試みも報告され
ている。Conventional technology Catalysts, enzymes/antigens, microorganisms, or animal/plant cells are attached or immobilized on a porous carrier to cause a chemical reaction.
A reaction system in which the porous material itself is a raw material for a chemical reaction is
In general, it is effective in facilitating the separation of products that have a large contact area. For example, 51 in an Erlenmeyer flask.
It has been reported that when actinomycetes are photographed and cultured using a square-sized foamed urethane cube as a carrier, the actinomycetes proliferate within the urethane carrier, making it easy to separate the microorganisms from components produced by microbial reactions. There have also been reports of attempts to increase the contact area by immobilizing hydrogen-producing bacteria on porous carriers.
しかしこのような従来の系では反応生産物が担体表面ま
たは内部に蓄積されるに従って反応速度が減少してくる
し、担体の大きさも大きく換を行なう必要がある。接触
面積を大きくする念めには多孔径を小さくすることが望
ましいがメタンガスや水素ガスの発生する系ではガスが
担体に保持され（貴拌などの方ではなかなか出てきにく
い。またその結果反応液の交換速度も遅くなり、微生物
の活性ががえって低下するという困難が生ずる。動植物
細砲の培養も細肩の高濃度化と反応液の迅速な交換とは
従来の方法では予備し、高い効率が期待しにくがった。However, in such conventional systems, the reaction rate decreases as reaction products accumulate on or inside the carrier, and the size of the carrier also needs to be significantly changed. In order to increase the contact area, it is desirable to reduce the pore diameter, but in systems where methane gas or hydrogen gas is generated, the gas is retained in the carrier (difficult to come out with noble stirring, etc.). This also slows down the exchange rate of the reaction solution, resulting in the difficulty of reducing the activity of microorganisms.In the cultivation of animal and plant artillery, the conventional method requires a high concentration of microorganisms and a rapid exchange of the reaction solution. Efficiency was less than expected.
発明の開示
本発明はこの欠点を除去する化学反応装置に係り化学反
応能を有し、弾性を有する多孔性素材を反応液中に配置
した反応系において、当該素材を間欠的に圧縮する装置
を備えていることを特徴とする。DISCLOSURE OF THE INVENTION The present invention relates to a chemical reaction device that eliminates this drawback.In a reaction system in which a porous material having chemical reaction ability and elasticity is disposed in a reaction liquid, the present invention provides a device for intermittently compressing the material. It is characterized by having
換言すると、本発明は、反応液中に配置した、化学反応
能を有し、弾性を有する多孔性素材を用いる化学反応で
あって、反応中、この多孔性素材をく９返し、好ましく
は間欠的に圧縮することを特徴とする化学反応方法を提
供する。In other words, the present invention relates to a chemical reaction using a porous material having chemical reaction ability and elasticity disposed in a reaction solution, and during the reaction, the porous material is repeatedly rotated, preferably intermittently. Provided is a chemical reaction method characterized by compression.
また、本発明は弾性を有する多孔性素材とこの素材の圧
縮装置を系中に備えたことを特徴とする化学反応装置を
提供し、好ましくは、圧縮装置がベローズを備えて、反
応系と外系とを遮断している。Further, the present invention provides a chemical reaction apparatus characterized by comprising a porous material having elasticity and a compression device for this material in the system, and preferably, the compression device is equipped with a bellows to connect the reaction system to the outside. The system is cut off.
さらに１多孔性素材の上部が多孔板で覆われこの多孔板
を介して圧縮装置に連結していてもよい。Furthermore, the upper part of the porous material may be covered with a perforated plate and connected to the compression device via this perforated plate.
上記のように、反応容器を外界から遮断するために上記
圧縮する装置がベローズを形成していると生化学系の反
応では都合がよい。As mentioned above, it is convenient for biochemical reactions that the compression device forms a bellows in order to isolate the reaction container from the outside world.
この場合多孔性担体に酵素・抗原類、微生物または動・
植物細抱を固定した多孔性素材を用いるとよい。また化
学反応または生化学反応がガス発生をともなう場合多孔
性素材を圧縮する１と素材内に蓄積されたまたは表面に
付着したガスは素材から分離されるので有効である。In this case, the porous carrier contains enzymes, antigens, microorganisms or
It is best to use a porous material with fixed plant holders. It is also useful when a chemical or biochemical reaction involves the generation of gas, since compressing the porous material 1 will separate the gases that have accumulated within the material or adhered to the surface from the material.
本発明の装置を用いると多孔により反応素材と反応液の
接触面積を大きくとることができ、反応生産物は圧縮操
作によシ素材内または表面から液中に排出され、分離さ
れる。この生成物を含む液の一部を反応容器から除き新
らしい未反応液を反応容器中に導入してのち圧縮を解除
すると素材が弾性によシ復元すると共に新しい反応液を
内部に含浸させ゛ることかできる。When the apparatus of the present invention is used, the contact area between the reaction material and the reaction liquid can be increased due to the pores, and the reaction products are discharged into the liquid from within or on the surface of the material by a compression operation and are separated. When a part of the liquid containing this product is removed from the reaction vessel and a new unreacted liquid is introduced into the reaction vessel, the compression is released, the material recovers elastically and the new reaction liquid is impregnated inside. I can do that.
本発明を放線菌を連続培養する実施例に従って詳細に説
明する。The present invention will be explained in detail according to an example in which actinomycetes are continuously cultured.
第１図は本発明に係る化学反応装置主には微生物反応装
置を示す。培養槽１の中に多孔性素材であるウレタン発
泡シート２が複数枚配電されている。パツキン５を介し
て培養ｉ？ａＫ固定さ１れている天板４にはベローズ５
の一端が固定されており、ベローズの他端には多孔板６
が固定されている。無 ベローズの筒内部の板７には多
孔は形成されておらず培養槽内は無菌に保ちうる。ベロ
ーズは駆動機構（図示せず）により上下動するようにな
っている培養液はりザーバータンク８と循環系を形成し
ている。すなわちポンプ９によって引き抜かれた培養液
はりザーパータンクへ送られる。リザーバータンクは通
常の培養装置のようにバックルグレート、スパージャ−
ターピー翼等を備えておシ、酸素供給と共に炭酸ガスの
ような生成した過剰なガス成分全放出し、またポンプに
より新鮮な培地の導入等ができるようになっている。こ
の培養槽１ヘホンプ１０により供給される。この循環系
からその一部はポンプ１１にょシ抜き取られ、液面が一
定に保たれるがこの系で間欠的に多孔板６を上下動する
ことにょジウレタン発泡シート内での生成物が循環液に
移動し新たな循環液が導入される。このようにして培養
すると放線菌は殆んどウレタン発泡シート内で増殖する
ので、菌体の分離が容易でしかも発泡シートの接触表面
積を有効に利用できる。メタンや水素ガスを発生する微
生物の培養においてはベローズの圧縮のみで発生ガスは
ほとんどすべて外部に放出され新たに新鮮な培地を供給
すれば、大きなガス発生速度が迅速に得られる。FIG. 1 shows a chemical reaction device, mainly a microbial reaction device, according to the present invention. A plurality of urethane foam sheets 2, which are porous materials, are electrically distributed inside the culture tank 1. Cultured via Patsukin 5? Bellows 5 are attached to the top plate 4 which is fixed to aK1.
One end of the bellows is fixed, and a perforated plate 6 is attached to the other end of the bellows.
is fixed. No pores are formed in the plate 7 inside the bellows cylinder, so the inside of the culture tank can be kept sterile. The bellows forms a circulation system with the culture solution reservoir tank 8, which is moved up and down by a drive mechanism (not shown). That is, the culture solution drawn out by the pump 9 is sent to the serpar tank. The reservoir tank has a buckle grate and sparger like a normal culture device.
It is equipped with tarpy blades and the like to supply oxygen and release all generated excess gas components such as carbon dioxide gas, and also allows for the introduction of fresh culture medium using a pump. This culture tank 1 is supplied by a pump 10. A part of it is extracted from this circulation system by the pump 11, and the liquid level is kept constant, but when the perforated plate 6 is moved up and down intermittently in this system, the products in the urethane foam sheet are transferred to the circulation liquid. new circulating fluid is introduced. When cultured in this manner, most of the actinomycetes grow within the urethane foam sheet, making it easy to separate the bacterial cells and making effective use of the contact surface area of the foam sheet. When culturing microorganisms that generate methane or hydrogen gas, almost all of the generated gas is released to the outside by simply compressing the bellows, and a large gas generation rate can be quickly obtained by supplying a fresh medium.
上記実施例の放線菌の代ヤに攪拌などの物理的外力に対
して脆弱な付着性動物細胞を用いると、必要なガス成分
はりザーバーで供給でき、培地の交換も容易であシ、ま
たベローズの上下運動は反応速度により刺部できかつ外
力としても極めて弱い力が加わるのみであるので、細胞
濃度を上げること及び培養の効率化につながる。If adherent animal cells, which are vulnerable to external physical forces such as agitation, are used instead of the actinomycetes in the above example, the necessary gas components can be supplied with a reservoir, the medium can be easily exchanged, and the bellows The up and down movement of the cell is caused by the reaction speed, and only an extremely weak external force is applied, leading to increased cell concentration and culture efficiency.
第２図は本発明に係る他の実施例の反応容器を示す。反
応容器は球状の多孔性素材１５を浮遊させた反応部１４
と該素材を含まない攪拌部１５に多孔板１６によって仕
切られており、攪拌部にはマグネット１７及び翼１８か
ら構成される攪拌子が備えられ、反応容器の下からモー
ターに直結したマグネット（図示せず）により駆動され
るこのような反応システムでは素材内の反応液の交換が
容易なる。FIG. 2 shows a reaction vessel according to another embodiment of the present invention. The reaction container has a reaction section 14 in which a spherical porous material 15 is suspended.
and a stirring section 15 that does not contain the material are separated by a perforated plate 16, and the stirring section is equipped with a stirring bar composed of a magnet 17 and blades 18, and a magnet directly connected to a motor from below the reaction vessel (Fig. In such a reaction system driven by a reactor (not shown), exchange of reaction liquid within the material is facilitated.
実施例では生化学反応について記したが本発明は触媒を
多孔性素材に吸着させた反応系や多孔性素材自身が反応
原料である反応系にも有効である。Although biochemical reactions have been described in the examples, the present invention is also effective in reaction systems in which a catalyst is adsorbed on a porous material, and in reaction systems in which the porous material itself is a reaction raw material.
第１図及び第２図は本発明方法及び装置の実施例を示す
。図中、
１ 反応槽
２ 多孔性素材
３ バッキング
４ 天板
５ ベローズ
６ 多孔板
７ 内部板
８ リザーバータンク
？ ポンプ
１０ ポンプ
１１ ポンプ
１２ フィルター1 and 2 show an embodiment of the method and apparatus of the invention. In the figure: 1 Reaction tank 2 Porous material 3 Backing 4 Top plate 5 Bellows 6 Perforated plate 7 Internal plate 8 Reservoir tank? Pump 10 Pump 11 Pump 12 Filter
Claims (1)
する多孔性素材を用いる化学反応であつて、反応中、こ
の多孔性素材をくり返し圧縮することを特徴とする化学
反応方法。 ２、圧縮を間欠的に行なうことを特徴とする特許請求の
範囲第１項に記載の方法。 ３、多孔性素材が多孔性担体に酵素・抗原 類、微生物または動植物細胞を固定したものであること
を特徴とする特許請求の範囲１項に記載の方法。 ４、弾性を有する多孔性素材とこの素材の圧縮装置を系
中に備えたことを特徴とする化学反応装置。 ５、圧縮装置がベローズを備えて、反応系と外系とを遮
断していることを特徴とする特許請求の範囲第４項に記
載の装置。 ６、多孔性素材の上部が多孔板で覆われこの多孔板を介
して圧縮装置に連結していることを特徴とする特許請求
の範囲第４項に記載の装置。[Claims] 1. A chemical reaction using a porous material having chemical reaction ability and elasticity placed in a reaction solution, characterized in that the porous material is repeatedly compressed during the reaction. A chemical reaction method that involves 2. The method according to claim 1, wherein the compression is performed intermittently. 3. The method according to claim 1, wherein the porous material is a porous carrier on which enzymes/antigens, microorganisms, or animal and plant cells are immobilized. 4. A chemical reaction device comprising a porous material having elasticity and a compression device for this material. 5. The apparatus according to claim 4, wherein the compression device includes a bellows to isolate the reaction system from the outside system. 6. The apparatus according to claim 4, wherein the upper part of the porous material is covered with a perforated plate and is connected to a compression device via the perforated plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18080086A JPH0697991B2 (en) | 1986-07-31 | 1986-07-31 | Chemical reaction method and apparatus using porous material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18080086A JPH0697991B2 (en) | 1986-07-31 | 1986-07-31 | Chemical reaction method and apparatus using porous material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6336783A true JPS6336783A (en) | 1988-02-17 |
| JPH0697991B2 JPH0697991B2 (en) | 1994-12-07 |
Family
ID=16089561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18080086A Expired - Lifetime JPH0697991B2 (en) | 1986-07-31 | 1986-07-31 | Chemical reaction method and apparatus using porous material |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0697991B2 (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5707868A (en) * | 1992-05-06 | 1998-01-13 | I.V.M.H. Recherche | Variable-volume reactor-type device and process for culturing cellular material |
| US6245555B1 (en) * | 1998-09-01 | 2001-06-12 | The Penn State Research Foundation | Method and apparatus for aseptic growth or processing of biomass |
| US6329196B1 (en) * | 1996-11-27 | 2001-12-11 | William Nevil Heaton Johnson | Methods and apparatus for enhancement of mass transfer of a fluid in a porous matrix system containing biomass |
| WO2003020871A3 (en) * | 2001-08-30 | 2003-07-10 | Arbomedics Gmbh | Method and device for the in vitro cultivation of cells |
| WO2004090091A3 (en) * | 2003-04-11 | 2004-12-23 | Arbomedics Gmbh | Body with a culture surface for in-vitro propagation of cells |
| WO2005003287A1 (en) * | 2003-06-30 | 2005-01-13 | Cytrix Technologies Limited | Methods of cell culture |
| WO2005033261A3 (en) * | 2003-10-03 | 2005-05-26 | Bionas Gmbh | Method and supply unit for monitoring changes and states in reaction chambers |
| JP2010041988A (en) * | 2008-08-12 | 2010-02-25 | Able Corp | Culture reactor |
-
1986
- 1986-07-31 JP JP18080086A patent/JPH0697991B2/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5707868A (en) * | 1992-05-06 | 1998-01-13 | I.V.M.H. Recherche | Variable-volume reactor-type device and process for culturing cellular material |
| US6329196B1 (en) * | 1996-11-27 | 2001-12-11 | William Nevil Heaton Johnson | Methods and apparatus for enhancement of mass transfer of a fluid in a porous matrix system containing biomass |
| US6245555B1 (en) * | 1998-09-01 | 2001-06-12 | The Penn State Research Foundation | Method and apparatus for aseptic growth or processing of biomass |
| US6709862B2 (en) | 1998-09-01 | 2004-03-23 | The Penn State Research Foundation | Growing cells in a reservoir formed of a flexible sterile plastic liner |
| WO2003020871A3 (en) * | 2001-08-30 | 2003-07-10 | Arbomedics Gmbh | Method and device for the in vitro cultivation of cells |
| WO2004090091A3 (en) * | 2003-04-11 | 2004-12-23 | Arbomedics Gmbh | Body with a culture surface for in-vitro propagation of cells |
| WO2005003287A1 (en) * | 2003-06-30 | 2005-01-13 | Cytrix Technologies Limited | Methods of cell culture |
| WO2005033261A3 (en) * | 2003-10-03 | 2005-05-26 | Bionas Gmbh | Method and supply unit for monitoring changes and states in reaction chambers |
| JP2010041988A (en) * | 2008-08-12 | 2010-02-25 | Able Corp | Culture reactor |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0697991B2 (en) | 1994-12-07 |
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