JPS6326166Y2 - - Google Patents

Description

[Detailed Description of the Invention] [Industrial Application Field] This invention relates to a liquid filter for obtaining a certain sample by passing a liquid sample through the microporous membrane surface of a hollow fiber.

[Conventional technology]

Conventionally, in this type of liquid filter, a hollow eye bar penetrates the bottom surface of the casing, and the liquid inside the casing can be extracted to the outside of the casing through the membrane surface of the hollow eye bar. The pore size is extremely small, and especially when filtering highly viscous liquid samples such as blood samples (whole blood, serum, etc.), it is impossible to filter them without using a centrifuge, and the top surface is open. However, it had become compatible with centrifuges.

Therefore, not only is it of little use in places without centrifugal separator equipment, but centrifugal separators are limited in the number of filters that can be applied to their disks at one time, making it difficult to obtain the required specimens from a wide variety of liquid samples. It took a lot of time.

[Purpose of the invention] In view of the above points, the purpose of this invention is to provide a liquid filter that can quickly and accurately obtain a necessary specimen without using a centrifuge.

[Structure of the idea]

In order to achieve the above object, this invention provides a liquid filter in which a hollow eye bar penetrates the bottom surface of the casing, and the liquid inside the casing can be extracted to the outside of the casing through the membrane surface of the hollow fiber eye. A cylindrical member is provided on the outer bottom surface of the housing to communicate through the membrane surface of the hollow eye bar, and a suction device connection port is provided on the cylindrical member, so that the inside of the cylindrical member can be subjected to negative pressure to filter and extract the liquid sample within the housing. This is what I did.

〔Example〕

Next, this invention will be explained based on embodiments shown in the accompanying drawings.

Reference numeral 1 denotes a cylindrical housing made of a transparent material (for example, plastic), and the housing 1 has a tubular protrusion 3 on its bottom surface 2. Reference numeral 4 denotes a hollow eye bar that penetrates the tubular protrusion 3 of the housing 1, and the hollow eye bar 4 is formed into a hollow shape with a membrane surface having countless micropores (molecular weight of about 10,000 to 100,000). be. The tubular protrusion 3 and the hollow eye bar 4 are completely sealed together by a suitable sealing member 5 to prevent leakage of gas and liquid.

Reference numeral 6 denotes a cylindrical member that communicates with the outer bottom surface of the housing through the membrane surface of the hollow fiber, and the cylindrical member 6 usually extends the tubular protrusion 3 outward as shown in the figure, or
A connecting port 9 is fitted on the outer periphery of the tubular protrusion 3, and a connecting port 9 into which a connecting end 8 of an aspirator (for example, a syringe) 7 can be inserted is provided at the outer end thereof, and by operating the aspirator 7, The interior is designed to have negative pressure. This suction device connection port 9 may be provided on the side surface of the cylindrical member 6.

The shape of the cylindrical member 6 differs depending on the state of penetration of the hollow eye bar 4 that passes through the tubular projection 3 provided on the bottom surface of the housing 1. Typical examples of this aspect are shown in FIGS. 2A to 2E.

In Figure A, most of the length of the hollow eye bar 4 is inside the housing 1, and its inner end 4a is sealed 10.
However, the outer end 4b is open, and the cylindrical member 6 is formed to extend the tubular projection 3. In this case, when a suction device is connected to the suction port connection port 9 provided at the outer end of the cylindrical member 6 and pulled to create a negative pressure inside the cylindrical member 6, the liquid sample a is transferred to the outer periphery of the hollow eye bar 4 as shown by the arrow. It enters the inner hole through the membrane surface and is filtered and collected into the cylindrical member 6 from the outer end 4b.

In FIG. B, most of the length of the hollow eye bar 4 is exposed to the outside of the housing 1, and the inner end 4a is open.
The outer end 4b is sealed 10, and the cylindrical member 6, which is longer than the exposed portion of the hollow fiber 4, is attached using the outer periphery of the tubular projection 3. Although this attachment means is of a screw type in the illustrated case, it may also be of a tapered fitting type. In this case, the cylinder member 6
When a suction device is connected to the suction device connection port 9 provided at the outer end of the case and pulled to create a negative pressure inside the cylindrical member 6, the liquid sample a in the housing 1 flows into the hollow eye 4 as shown by the arrow.
The liquid enters the inner hole from the inner end 4a of the membrane, passes through the membrane surface from the inner hole to the outer periphery, and is filtered and collected in the gap between the cylinder member 6 and the sealed outer end 4b.

In Figure C, the lengths of the inside and outside of the casing 1 of the hollow eye bar 4 are approximately the same, and both the inside and outside ends are sealed 10. It is the same as. In this case, the cylinder member 6
When the pressure is set to negative, the liquid sample a inside the housing 1 enters the inner hole from the outer periphery of the hollow fiber 4 through its membrane surface as shown by the arrow, and passes through the outer end 4b sealed outside the housing. The membrane surface is sampled from the inside of the inner hole to the outer periphery. That is, the liquid sample a passes through the membrane surface of the hollow eye bar 4 twice and is filtered.

In Figure D, an even number of tubular protrusions 3 are provided on the bottom surface of the housing 1, and both ends of U-bent hollow fibers 4 are passed through the tubular protrusions 3 from the inside of the housing 1 to the outside. Both ends of a U-bent hollow fiber 4 are passed through the housing 1 from the outside to the inside, and the cylindrical member 6 is attached to the outer periphery of the lower end of the housing 1. This mounting method is not limited to screw type,
A taper fitting type or a lock type may be used. In this case, since the outer diameter of the cylindrical member 6 becomes large, it is preferable to provide the tubular projection 6' at the outer end and the suction device connection port 9 at the inner or outer periphery thereof. In this case D, filtration of liquid sample a is carried out using hollow eye bars 4
This is the same as in case A above, in which the inner end of the tube is sealed.
On the other hand, case E is the same as case B above, in which the outer end of the hollow eye bar 4 is sealed.

Note that the shape of the housing 1 itself and the shape of the cylindrical member 6 are not limited to those shown in the drawings.

Further, in the above embodiment, the tubular member 6 uses the tubular protrusion 3 on the outer bottom surface of the housing 1 to attach the hollow eye bar 4, and is attached by extending the protrusion 3 or using its outer periphery. Attachment means for the cylindrical member 6 may be provided on the outer bottom surface of the casing, independent of the tubular projection 3 as described above.

In the above embodiment, an appropriate amount of liquid sample is injected into the housing 1, and the suction device is a cylindrical member 6 in which the connection end 8 of the suction device 7 is connected to the outer bottom surface of the housing 1 through the membrane surface of the hollow eye bar 4. When connected to the connection port 9 and suctioned, the inside of the cylindrical member 6 becomes negative pressure. As a result, the sample inside the housing has an extremely fine pore size on the membrane surface of the hollow eye bar 4, and especially when the liquid sample is whole blood or
Even if it is a highly viscous substance such as serum, its membrane surface can be
The sample is passed through once or twice, filtered, and received into the suction device 7, where it can be used to collect a sample. in this case,
When a syringe is used as the suction device 7, it is convenient to use a suitable jig (not shown) to hold the plunger in the suction position.

[Effect of idea]

As described above, the liquid filter according to this invention is a liquid filter in which the hollow eye bars penetrate the bottom surface of the casing, and the liquid inside the casing can be extracted to the outside of the casing through the membrane surface of the hollow eye bars.
The gist is that a cylindrical member communicating with the outer bottom surface of the casing via the membrane surface of the hollow fiber is provided, and a suction device connection port is provided in the cylindrical member.
Not only is it possible to collect specimens in places without centrifuge equipment, but there is no limit to the number of filters that can be applied to a disk at one time, unlike when using a centrifuge. It has various excellent effects, such as being able to quickly obtain the required specimen from each liquid sample.

[Brief explanation of drawings]

The figures show an embodiment of this invention. Fig. 1 is a cross-sectional view, and Figs. 2 A to E are schematic explanatory views showing the installation mode and filtration state of the hollow eye bar. If it is inside the body, B means that most of the hollow eye bar is outside the casing, C means that the hollow eye bar extends approximately the same length both inside and outside the casing, and D means that both ends of the U-bent hollow eye bar are inside the casing. E is a case where both ends of the U-bent hollow fiber eye are penetrated through the bottom surface from outside the casing. 1... Housing, 2... Bottom surface, 3... Tubular projection, 4
... Hollow eye bar, 5 ... Sealing member, 6 ...
Cylindrical member, 8...Suction device, 9...Suction device connection port.

Claims (1)

[Scope of utility model registration request] In a liquid filter in which a hollow eye bar penetrates the bottom surface of the casing and the liquid inside the casing can be extracted to the outside of the casing through the membrane surface of the hollow eye bar, the membrane surface of the hollow eye bar is provided on the outer bottom surface of the casing. A liquid filter characterized in that a cylindrical member is provided which communicates through the cylindrical member, and a suction device connection port is provided in the cylindrical member.