JPS63231256A - Calibration data processing method for sample electrolyte measurement using ion-selective electrodes - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

Detailed Description of the Invention [Industrial Application Field] The present invention measures the potential between a reference electrode and an ion-selective electrode for a standard solution and a sample, calculates a calibration potential from the measured potential of the standard solution, and calculates the calibration potential for the sample. This invention relates to a calibration data processing method for measuring electrolyte in a sample using an ion-selective electrode that measures the amount of electrolyte in a sample.

[Conventional technology]

FIG. 4 is a diagram showing an example of a blood electrolyte measurement system using an ion-selective electrode, in which 11 is a sampler, 12 and 2
0 is a pump, 13 is a flow cell, 14 is an ion selection bottle, 15 is a reference electrode, 16 is a controller, 17 is an amplifier, 18 is an ADC (analog-digital converter), 1
9 is a data processing section, and 21 is a drain tank.

In FIG. 4, a potential difference corresponding to the electrolyte star in the blood to be measured is obtained between the ion selection electrode 14 and the reference electrode 15. Therefore, under the control of the controller 16, the flow cell 13 is By switching between sending the standard solution and the sample and taking their potentials into the data processing unit 19 through the amplifier 17 and ADC 18, the amount of electrolyte in the sample can be measured using the measured potential of the standard solution as a calibration potential. Blood electrolytes measured include Na”
, K", Ca", C1-, Co, "-, etc., -m
High detection accuracy is required.

FIG. 5 is a diagram showing the operation timing and output waveform of the blood electrolyte measurement system, and FIG. 6 is a diagram showing an example of the configuration of the data processing section.

In the above blood electrolyte measurement system, when the controller 16 switches the sampler 11, pump 12 (PZ), 20 (P, ) according to the operation timing shown in FIG. Corresponding to this operation, a potential difference as shown in FIG. 5 (bl) appears between the ion selection electrode 14 and the reference electrode 15.

Therefore, in the data processing section, this potential difference is held in the hold circuit 22 according to the measurement timing signal from the controller 16, as shown in FIG. 6, and the measured values of the standard solution and the sample are sequentially stored in the register 23. Then, the arithmetic circuit 24 calculates the concentration of the specimen using the measured potential of the standard solution as a calibration potential and stores it in the file 25.

In measuring a sample electrolyte using an ion-selective electrode such as the one described above, calibration is performed using an internal standard solution every time one sample is measured or every several samples are measured in order to improve measurement accuracy in any model. Therefore, when measuring specimens with the same concentration, level signal waveforms of the specimen and internal standard solution as shown in FIGS. 7(al and bl) are obtained.

[Problem that the invention seeks to solve]

However, in the above-mentioned sample electrolyte measurement, depending on the type of electrode, the calibration potential of the internal standard solution does not always remain constant when measurements are repeated, and as shown in Figure 8, the calibration potential is not always the same as the initial potential. In some cases, a drift gradually upwards from 1- is observed. Possible causes of this include the response characteristics of the electrode, adsorption of special components in the sample, and temperature fluctuations. This tendency is particularly noticeable in the C1 electrode using a quaternary ammonium ion exchanger. This drift has a considerable effect on measurement accuracy, and the effect is particularly significant when the measurement speed is increased.

The present invention solves the above-mentioned problems, and provides a calibration data processing method for analyte electrolyte measurement using an ion-selective electrode that provides high measurement accuracy even if there is a drift in the calibration potential of an internal standard solution. The purpose is to provide

[Means for solving problems]

For this purpose, the calibration data processing method for sample electrolyte measurement using the ion-selective electrode of the present invention measures the potential between the reference NFfx and the ion-selective electrode for the standard solution and sample, and calculates the calibration potential from the measured potential of the standard solution. This is a calibration data processing method for sample electrolyte measurement using an ion-selective electrode that calculates the amount of electrolyte in the sample and measures the amount of electrolyte in the sample, and is characterized by calculating the calibration potential from the measured potential of a standard solution before and after sample measurement. do.

[Effect]

In the calibration data processing method for analyte electrolyte measurement using the ion-selective electrode of the present invention, the calibration potential is calculated from the measured potential of the standard solution before and after the analyte measurement, so it is possible to find the corresponding calibration potential at the time of the analyte measurement. can. Furthermore, even if the drift differs depending on various measurement conditions, more accurate measurement can be achieved by approximating the drift using a curve or the like to obtain the calibration potential.

〔Example〕

Examples will be described below with reference to the drawings.

FIG. 1 is a diagram for explaining one embodiment of the calibration data processing method for measuring analyte electrolytes using the ion-selective electrode according to the present invention, and FIGS. 2 and 3 are diagrams showing examples of calculation of the calibration potential. It is.

In FIG. 1, l is the measuring device, 2 is the controller, and 3
is a hold circuit, 4 is a shift register, 5 is an arithmetic circuit,
6 indicates a file. The measuring device 1 is a blood electrolyte measuring device using an ion selectivity 1 pole as shown in FIG. 4, and the measuring device 1, controller 2, and hold circuit 3 are as follows:
Each is the same as in Figure 6. The shift register 4 stores measurement data at three points, and under the control of the controller 2, the hold circuit 3 stores the measured potential of the standard solution,
When the measured potential of the specimen and the measured potential of the standard solution are held in sequence, the data of these three points are stored in the shift register 4. The calculation circuit 5 reads these data, calculates a calibration potential from the measured potentials of the standard solution before and after the specimen, and calculates and measures the potential of the specimen, that is, the amount of electrolyte, based on the calibration potential.

Here, the standard and night measurement potential after the sample measurement is shifted by the shift register 4 to become the measurement potential of the previous standard solution for the next sample, and when the standard solution is measured following that sample. Calculation of the calibration potential and calculation and measurement of the amount of electrolyte are performed in the same manner.

Furthermore, to explain the calibration process in detail, when calibrating with the internal standard solution each time, as shown in Figure 2+a+, not only the calibration potential A before sample measurement, but also the calibration potential C after sample measurement.
(In the conventional method, the calibration potential of the next specimen) is also used, and the difference between them is regarded as a drift of the Haese line to calculate the calibration potential at the specimen measurement point. Normally, when samples with different physical properties (an aqueous solution and a serum sample, a sample with a temperature difference, etc.) are introduced into the detection section, they drift as shown in FIG. 2('b).

From this, by simulating and calculating this waveform as shown in Figure 2 (curve β in al) and calculating the calibration potential, it becomes possible to calculate the constituent potential in accordance with the measurement conditions. A numerical value may be used depending on the measurement conditions, or a simulation curve may be set in advance.

In addition, for the drift, it is not necessary to use the above-mentioned simulation music Mp1 (but it is also possible to use the direct 23 pz shown in FIG. 2 (al) or FIG. 3. You may take the middle point of C, or you may take the time point corresponding to the sample measurement point.

Note that the present invention is not limited to the above-mentioned embodiment (and various modifications are possible. For example, in the above-mentioned embodiment, the measured potential at each point is stored using a hold circuit or a shift register. However, after controlling the ADC according to all constant timing and sequentially importing the measured potential data of the standard solution and sample output from the ADC into the memory, the potentials before and after the sample measurement point are read out and the calibration potential is calculated. Alternatively, other methods may be adopted.Furthermore, although the case of drift up has been described, it goes without saying that calibration can be performed for drift down by performing similar calculations.

C. Effects of the Invention] As is clear from the above description, according to the present invention, when performing calibration with an internal standard solution, the calibration potential is obtained by performing calculations using the measured potentials of the internal standard solution before and after sample measurement. Therefore, accurate calibration can be performed according to the conditions under which drift occurs, and more accurate measurement can be performed.

[Brief explanation of the drawing]

FIG. 1 is a diagram for explaining one embodiment of the calibration data processing method for measuring analyte electrolytes using the ion-selective electrode according to the present invention, and FIGS. 2 and 3 are diagrams showing examples of calculation of the calibration potential. ,
Figure 4 shows an example of a blood electrolyte measurement system using ion-selective electrodes, Figure 5 shows the operation timing and output waveform of the blood electrolyte measurement system, and Figure 6 shows the data processing unit. FIG. 7 is a diagram showing an example of a measurement waveform, and FIG. 8 is a diagram showing an example of a measurement waveform in which drift is observed. DESCRIPTION OF SYMBOLS 1... Measuring device, 2... Controller, 3... Hold circuit, 4... Shift register, 5... Arithmetic circuit, 6... File. Applicant: JEOL Co., Ltd. Agent: Patent attorney Ryukichi Abe (2 others) Figure 2: Cow diagram

Claims (2)

[Claims] (1) Sample electrolyte using an ion-selective electrode that measures the potential between the reference electrode and the ion-selective electrode for the standard solution and the sample, calculates the calibration potential from the measured potential of the standard solution, and measures the amount of electrolyte in the sample. A calibration data processing method for measurement of a sample electrolyte using an ion-selective electrode, the method comprising calculating a calibration potential from the measured potential of a standard solution before and after measurement of the sample. (2) The ion selective electrode according to claim 1, wherein a transition line is set between the measurement potentials of the standard solution before and after the sample measurement, and a point on the transition line is set as a calibration potential. Calibration data processing method for sample electrolyte measurement used.