JPS6317046B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to microbiology. For more details,
TECHNICAL FIELD The present invention relates to a novel biological preparation for stimulating the vital activity of living organisms and a method for producing the same.

For example, biological enzyme preparations prepared by culturing Bacterium acidi propionicum in a nutrient medium consisting of skimmed milk, animal products, milk hydrolyzate, calcium chloride and trace elements. Pharmaceutical formulations and methods for their production are known. In this case, fermentation is first carried out at 20°C and then at 35°C, and then the medium is neutralized with an aqueous salt solution such as sodium carbonate. Isolation of the preparation is carried out using conventional methods (see Soviet inventor's certificate no. 186941).

In addition, Bacillus mesentericus (Bacillus mesentericus) can be grown in a medium consisting of skim milk, milk hydrate, and rennin.
Enzyme preparations are also known which are prepared by culturing A. mesentericus) and then adding fresh skim milk so that the nutrient medium is not depleted during fermentation. In this case, at the end of the microbial growth step, the medium is aerated, and the enzyme preparation is then recovered from the culture mixture by conventional methods such as precipitation. As a precipitant, a legume powder pretreated with an organic acid such as lactic acid is used. If necessary, the obtained enzyme preparation is dried (USSR inventor's certificate No. 186942)
(see issue).

The aforementioned prior art formulations do not contain adequate amounts of amino acids and essential vitamins and enzymes essential for the vital activities of living organisms.

Biological preparations are also known which are prepared by adding bacterial enzymes consisting of acidophilic bacteria to a nutrient medium followed by fermentation. In this case, the nutrient medium consists of animal material, skim milk powder, wasp larvae and cocoons, salt and additives such as cobalt chloride and benzoic acid.

Initial fermentation and hydrolysis of milk proteins and animal materials takes place at 15-20°C for 36-48 hours, and final hydrolysis takes place at 40°C for about 36-48 hours. The medium must be stirred periodically during fermentation. After fermentation, the biological product is separated and the resulting biological product is recovered. This preparation is then filtered, cooled and packaged (see USSR Inventor's Certificate No. 282052).

This prior invention method does not allow the preparation of biological preparations containing a balanced amino acid composition and appropriate amounts of enzymes, vitamins and trace elements.

The purpose of the present invention is to produce a new highly efficient biological preparation having amino acid, enzyme, vitamin and trace element contents suitable for the life activities of living organisms.

Another object of the present invention is to enhance the method of manufacturing this biological product.

The present invention provides amino acids, enzymes, and enzymes suitable for the life activities of living organisms by selecting a novel bacterial enzyme, a nutrient medium, appropriate fermentation conditions, and a method for producing the nutrient medium.
It is an object of the present invention to provide highly efficient biological preparations with vitamin and trace element content and to provide an enhanced method for the production of said biological preparations.

For these and other purposes, Lactobacterium is added to a nutrient medium containing sources of carbon, nitrogen and inorganic salts and biologically active compounds.
Bacterial enzymes consisting of bacteria of the genus
(b) Lactobacterium bacteria as a bacterial enzyme, and Hyperthermic bacteria of the genus Streptobacterium and the genera Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum
Bacteria obtained by long-term symbiosis and adaptation of a mixture of mesophilic bacteria of the genera Proprionicum, Escherichia coli, and Pseudomonas to a nutrient medium enriched with carbon, nitrogen, and inorganic salt sources and biologically active compounds. It is a preparation manufactured using enzyme-producing enzyme, and (b) total protein content is 6-26% by weight, pH is 3.2-3.8, and Turner's acidity is 6-26% by weight.
This is achieved by providing a biological preparation characterized by an acidity of 600 to 900°.

The biological preparation of the present invention comprises amino acids (lysine,
histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine),
It contains various enzymes, vitamins, carbohydrates, various low molecular weight compounds, and trace elements, that is, all the basic components essential for the life activities of living organisms.

The use of the biological preparation of the invention as a food product ensures the dynamic constancy of the composition of the internal body medium throughout the cell, increasing its energy level, and furthermore, there is no discontinuity between proteins and enzymatic cell exchange. It is possible to prevent equilibrium from occurring.

The formulations of the invention can be used both in liquid state (homogenized broth) and in the form of pastes and dry powders (tablets). The liquid formulation of the present invention can be mixed with edible bulking agents such as soybean flour, rice flour or mixtures thereof and dried at room temperature. This biological product was tested as a food product and as a stimulant of biological activity for humans and animals (pigs, piglets, calves).

When administered to humans, the biological preparations of the present invention produce healthier appetite and sleep, reduce fatigue, produce a more cheerful mood, and increase work capacity.

The biological preparation of the present invention causes a 5-10% weight gain when administered to animals with feed;
It activates behavior, increases motility, and improves the taste of meat.

The invention also provides for pre-preparing a nutrient medium containing carbon, nitrogen and inorganic salt sources and biologically active compounds;
A biological preparation comprising adding bacterial leaven consisting of bacteria of the genus Lactobacterium to the nutrient medium and fermenting it, and then separating the culture solution and the biological product. In the production method, (a) as the bacterial enzyme, bacteria of the genus Lactobacterium, hyperthermic bacteria of the genus Streptobacterium, genus Streptococcus, genus Azotobacterium, Shermanni, Bacterium acidi propionicum, Escherichia coli, and a mixture of mesophilic bacteria of the Pseudomonas genus are used as carbon, nitrogen, and inorganic salt sources and biologically active compounds. Using bacterial enzymes obtained by acclimatization to a nutrient medium enriched with
1 to 1.5 days at 15°C, then 20 to 50°C
1 to 1.5 days.

Preferably, the bacterial enzyme is added to the nutrient medium in an amount of 2 to 5% by volume. As the nutrient medium, any suitable nutrient medium may be used provided that it is enriched with carbon, nitrogen and inorganic salt sources and trace elements.

Preferably, a nutrient medium having the following composition is used.

(Weight%) Milk 30-35.0 Rennin 2.0-3.0 Pork by-products 19.0-21.0 Cobalt sulfate 0.002-0.004 Poultry eggs 1.5-2.0 Fish 1.0-3.0 Salt 0.8-1.0 Ethanol 0.04-0.0 5 Potassium chloride 0.4-0.5 Marble 1.8-2.0 Honey 1.0 to 1.5 Bee food 0.1 to 0.15 Manganese sulfate 0.002 to 0.004 Lake mud 1.8 to 2.0 Addition of legumes 100 These ingredients are used in nutrient media because they are the primary source of nutrients for the microflora. This is due to the fact that the aforementioned ingredients are utilized first. Legumes, fish and bird eggs are used as protein sources, honey and bee food are used as carbohydrate sources, manganese sulfate, cobalt sulfate and cobalt chloride serve as trace element sources, and marble provides a buffer system. It is also used as a calcium source.

To achieve mechanical activation of the nutrient medium addition components, the medium is disrupted at a striking speed of 100 to 300 m/sec before adding the bacterial enzyme.

Such disruption of the nutrient medium can accelerate the fermentation process.

In order to homogenize the biological product and improve its quality, the culture medium is also disrupted at a striking speed of 100 to 300 m/s.

The method of the present invention is carried out as follows. This method may be carried out batchwise or continuously.

First of all, prepare a nutrient medium. As the nutrient medium, any suitable medium enriched with proteins, carbohydrates, inorganic salts and trace elements may be used. Protein sources include milk, rennin,
Pork by-products, eggs, poultry, fish, and other suitable animal products obtained from freshly slaughtered healthy animals (pork by-products, pig stomachs, etc.) can be used. These are rinsed with cold water to remove foreign matter before use. Protein and sugar sources include a variety of legumes, including medicinal plants such as irakusa, dandelion, inondo, caraway, eucalyptus, schizandra, cypress, radish, turnip, etc. Plants (100
species) can be used. Plant seeds and pollen can be used as well as stems, leaves and roots. Honey and bee plants are used as carbohydrate sources. Metal salts and metal oxides are used as sources of trace elements.

Mix all components of the nutrient medium. For mechanical activation, the components are preferably disintegrated before fermentation at a striking speed of 100 to 300 m/s. Fermentation is carried out with bacterial enzymes added in an amount of 2 to 5% by volume of the nutrient medium. Bacterial enzymes include thermophilic bacteria of the genera Lactobacterium and Streptobacterium, as well as Streptococcus, Azotobacterium, Schermanni, and Bacterium acidii, which have been adapted to enriched nutrient media by long-term symbiosis. It comprises a mixture of various microorganisms, such as mesophilic bacteria of the genus Propionicum, Escherichia coli, and Pseudomonas. Fermentation is carried out at -5 to 15℃.
For 1 to 1.5 days, then at 20 to 50°C for 1 to 1.5 days.

During the fermentation process at low temperatures of -5 to +15°C, the basic components of the substrate are fermented by mesophilic bacteria, and at high temperatures of 20 to 50°C, the main fermentation is carried out by thermophilic bacteria.

After fermentation is complete, the culture broth is separated from the biological products. This biological product is used as a bacterial enzyme in the next cycle of biological product manufacturing.

Strain the collected culture fluid and use it to improve the quality of the biological product and ensure homogenization.
It is sufficient to crush the material at a striking speed of 100 to 300 m/sec.

The biological preparations of the present invention may be used in liquid form or as a dry product with food bulking agents. The resulting biological preparation has a characteristic color and aroma, depending on the composition of the botanical material and the selection of other nutrient medium components.

In order to provide a better understanding of the present invention, the present invention will be described with reference to the following examples.

Example 1 A nutrient medium with the following composition was prepared.

(Weight%) Milk 30.0 Rennin 3.0 Pork by-products 20.0 Cobalt sulfate 0.004 Bird eggs (poultry eggs) 2.0 Fish 3.0 Salt 1.0 Ethanol 0.05 Potassium chloride 0.5 Marble 2.0 Honey 1.5 Bee food 0.15 Manganese sulfate 0.004 Lake mud 2.0 Ma Meataceous plants How about soybeans, clover,
Remaining amounts of flowers, roots, stems, and leaves of locust beans, licorice) All of the above components were cleaned and put into a crusher, where they were mixed, crushed, and activated at a striking speed of 170 m/sec. One ton of the homogeneous product obtained by the above treatment was placed in a bath to which 5% by volume (based on the nutrient medium) of enzyme was previously added. The enzymes used were Lactobacterium and Streptobacterium.
Hyperthermic bacteria of the genera Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum,
It consists of a mixture of mesophilic bacteria of the genus Escherichia coli and Pseudomonas, and is produced by allowing these mixtures to coexist for a long time and adapting to an enriched nutrient medium.

Fermentation was carried out at a temperature of 10°C for 28 hours and then at 40°C for 24 hours. At the completion of fermentation, 40% of the total volume of the middle layer consisting of the culture solution was removed and the culture solution was strained.
The obtained biological residue was used as enzyme for the next production cycle. Strained culture solution is striking speed
It was crushed at 170 m/sec and then packaged. The obtained biological product has a pH of 3.2, Turner acidity
600, a homogeneous dark and colored liquid with a total nitrogen content of 6.9%. This preparation contains amino acids (lysine, histidine, arginine, aspartic acid, threonine,
serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine), complexes of enzymes and various hydrolyzing exoenzymes, peptides, polysaccharides, vitamins, and calcium, potassium, It contained trace elements such as sodium and magnesium.

Example 2 A nutrient medium with the following composition was prepared.

Weight% Milk 20.0 Rennin 2.0 Pig stomach 8.0 Pork by-products 12.0 Poultry eggs 2.0 Salt 1.0 Fish 5.0 Honey 1.0 Bee food 1.0 Flower flour 1.0 Cereal seeds (corn, rye, rice)
6.0 Cobalt sulfate 0.004 Chromium oxide Cr ₂ O ₃ 0.001 Titanium oxide TiO ₂ 0.001 Potassium chloride 0.5 Ethanol 0.2 Plant stems, leaves, roots (nettles,
Dandelion, inondo, caraway, eucalyptus, schizandra, kanokosou,
All of the above ingredients were cleaned and put into a crusher, where they were mixed, crushed and activated at a striking speed of 250 m/sec. One ton of the homogeneous product obtained by the above treatment was placed in a bath to which 5% by volume (based on the nutrient medium) of the enzyme (as in Example 1) had been added.

Fermentation was carried out at a temperature of 5°C for 25 hours and then at 35°C for 36 hours. At the completion of fermentation, the middle layer consisting of the culture solution was removed by 50% of the total volume and the culture solution was strained.
The obtained biological residue was used in the next production cycle as a enzyme. Strained culture solution is striking speed
It was crushed at 200 m/sec and then packaged. The obtained biological product has a pH of 3.8, Turner acidity
700, a homogeneous red colored liquid with a total nitrogen content of 11%. This preparation contains amino acids (lysine, histidine, arginine, aspartic acid, threonine,
Glutamic acid, proline, glycine, alanine,
Valine, methionine, isoleucine, leucine,
tyrosine, phenylalanine), enzyme peptide,
It contained polysaccharides, vitamins, and trace elements such as calcium, potassium, sodium, cobalt, chromium, and titanium.

Example 3 A nutrient medium with the following composition was prepared.

Weight% Milk 35.0 Rennin 2.0 Pork by-products 19.0 Cobalt sulfate 0.003 Poultry eggs 1.5 Fish 2.5 Salt 0.8 Ethanol 0.04 Potassium chloride 0.4 Marble 1.8 Honey 1.0 Bee food 0.1 Manganese sulfate 0.002 Glue bottom mud 1.8 Legumes ( Peas, soybeans, clover ,
Flowers, leaves, roots, and stems of locust bean, kanzo
Remaining Amount The above components were treated in the same manner as in Example 1. Fermentation is
15°C for 30 minutes, then 45-50°C for 28 minutes.
The obtained biological preparation was similar to Example 1. This liquid biological preparation was mixed with soybean flour to form a paste and dried at room temperature. The total protein content of the resulting desiccant was 25.4%.

Claims (1)

[Scope of Claims] 1. Bacterial leaven consisting of bacteria of the genus Lactobacterium is added to a nutrient medium containing carbon, nitrogen and inorganic salt sources and biologically active compounds and fermented. A biological preparation obtained by separating a biological product obtained from a culture from a culture solution, which contains (a) bacteria of the genus Lactobacterium and hyperthermia of the genus Streptobacterium as bacterial enzymes; Mesophilic bacteria and the genera Streptococcus, Azotobacterium, Shermanni, Bacterium acidi propionicum, Escherichia coli, and Pseudomonas A preparation produced using bacterial enzymes obtained by long-term symbiosis of a mixture of bacteria and adaptation to a nutrient medium enriched with carbon, nitrogen and inorganic salt sources and biologically active compounds,
and (b) total protein amount 6-26% by weight, PH3.2-3.8,
A biological preparation characterized by a Turner's acidity of 600 to 900°. 2. Preparing a nutrient medium containing carbon, nitrogen and inorganic salt sources and biologically active compounds, adding bacterial leaven consisting of bacteria of the genus Lactobacterium to the nutrient medium and fermenting it; In a method for producing a biological preparation, which comprises then separating a culture solution and a biological product, (a) bacteria of the genus Lactobacterium and hyperthermia of the genus Streptobacterium are used as bacterial enzymes; sexual bacteria and the genera Streptococcus, Azotobacterium, Shermanni, Bacterium acidi
Bacteria obtained by long-term symbiosis and adaptation of a mixture of mesophilic bacteria of the genera Proprionicum, Escherichia coli, and Pseudomonas to a nutrient medium enriched with carbon, nitrogen, and inorganic salt sources and biologically active compounds. 1. A method for producing a biological preparation, which uses an enzyme and (b) fermentation is carried out at -5 to +15°C for 1 to 1.5 days and then at 20 to 50°C for 1 to 1.5 days. 3. The method according to claim 2, wherein the bacterial enzyme is added in an amount of 2 to 5% by volume of the nutrient medium. 4. The method according to claim 2 or 3, wherein a medium having the following composition is used as a nutrient medium. (Weight%) Milk 30-35.0 Rennin 2.0-3.0 Pork by-products 19.0-21.0 Cobalt sulfate 0.002-0.004 Poultry eggs 1.5-2.0 Fish 1.0-3.0 Salt 0.8-1.0 Ethanol 0.04-0.0 5 Potassium chloride 0.4-0.5 Marble 1.8-2.0 Honey 1.0 to 1.5 Bee food 0.1 to 0.15 Manganese sulfate 0.002 to 0.004 Lake bottom mud 1.8 to 2.0 Addition of legumes 100 5. Patent for disrupting the nutrient medium at a striking speed of 100 to 300 m/s before adding bacterial enzymes The method according to any one of claims 2 to 4. 6 Claims 2 to 5, in which the culture fluid separated from biological products is disrupted at a striking speed of 100 to 300 m/sec for homogenization and quality improvement of biological products. The method described in any of the above.